ライフサイエンスコーパス: CRISPR-Cas9 system

1 ficient loss-of-function screening using the CRISPR-Cas9 system.

2 and targeted gene mutation in plants via the CRISPR-Cas9 system.

3 are known to inhibit the widely used class 2 CRISPR-Cas9 system.

4 anscriptional activation screening using the CRISPR-Cas9 system.

5 ivator-like effector (TALE) modules, and the CRISPR-Cas9 system.

6 that underlie the mechanism of action of the CRISPR-Cas9 system.

7 e model of Klhl31 loss of function using the CRISPR-Cas9 system.

8 ble knockout (DKO) rats were generated using CRISPR-Cas9 system.

9 a nucleosome, constrains the activity of the CRISPR-Cas9 system.

10 different strategies to edit genes using the CRISPR-Cas9 system.

11 the naturally occurring Type II prokaryotic CRISPR-Cas9 system.

12 rom NOD/SCID/IL2rg(-/-) (NSI) mice using the CRISPR/Cas9 system.

13 larger than 9 kb at the pH11 locus using the CRISPR/Cas9 system.

14 ies to optimize knockout efficiency with the CRISPR/Cas9 system.

15 ting and multiplex editing capability of the CRISPR/Cas9 system.

16 with mosaic anxa4 knockout studies using the CRISPR/Cas9 system.

17 ITPR2-knockout HepG2 cells generated by the CRISPR/Cas9 system.

18 ations in the iPSCs were corrected using the CRISPR/Cas9 system.

19 y of a single-stranded oligonucleotide and a CRISPR/Cas9 system.

20 larly interspaced short palindromic repeats (CRISPR)-Cas9 system.

21 the remarkable mechanistic diversity of the CRISPR-Cas9 systems.

22 d divergent features among distantly related CRISPR-Cas9 systems.

23 c applications through AAV-based delivery of CRISPR/Cas9 systems.

24 ng can be achieved by combining the TAEL and CRISPR/Cas9 systems.

25 ort Palindromic Repeats/Crispr associated 9 (CRISPR/Cas9) system.

26 order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activate

27 We show that this homing CRISPR-Cas9 system acts as an expressed genetic barcode

28 hylococcus aureus cells harbouring a type II CRISPR-Cas9 system after infection with the staphylococc

29 The CRISPR/Cas9 system allows for such targeted mutagenesis,

30 The bacterial CRISPR/Cas9 system allows sequence-specific gene editing

31 ularly interspaced short palindromic repeat (CRISPR)-Cas9 system and measured the quantities of bindi

32 ably, NRP1 knockdown with interfering RNA or CRISPR-cas9 system and blocking using anti-NRP1 antibody

33 have been drawn between the newly discovered CRISPR-Cas9 system and the RNA interference (RNAi) pathw

34 improve our mechanistic understanding of the CRISPR-Cas9 systems and may facilitate Cas9 engineering.

35 nts in the genome editing specificity of the CRISPR/Cas9 system and enabled its activation with tempo

36 RNA of Mycoplasma mycoides, by combining the CRISPR/Cas9 system and the yeast recombination machinery

37 epeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system and the turquoise killifish genome,

38 ularly interspaced short palindromic repeat (CRISPR)/Cas9 system, and in vivo selection for aminoglyc

39 CRISPR/Cas9 systems are a versatile tool for genome edit

40 larly interspaced short palindromic repeats (CRISPR)/Cas9 system, are systematized for fast, modular

41 cent proof of principle experiments with the CRISPR-Cas9 system as a drive mechanism.

42 The adaptation of the CRISPR-Cas9 system as a genome editing technique has gen

43 These results unveil the potential of the CRISPR/Cas9 system as a new therapeutic strategy against

44 ic modification and further expansion of the CRISPR/Cas9 system as a spatiotemporally controlled gene

45 zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for genome editing, tran

46 Delivery of components of the CRISPR-Cas9 system by hydrodynamic injection resulted in

47 Here, we demonstrate that the CRISPR-Cas9 system can be used to edit the VACV genome r

48 for future antibiotic therapies and that the CRISPR-Cas9 system can introduce rapid and efficient mod

49 infections, and we demonstrate here that the CRISPR/Cas9 system can be adapted for antiviral treatmen

50 Here we show that the CRISPR/Cas9 system can restrict the JCPyV life cycle in

51 Here, we show that the CRISPR/Cas9 system can specifically target and cleave co

52 systems, such as the Streptococcus pyogenes CRISPR-Cas9 system, can be adapted such that Cas9 can be

53 ls by combining rAAV6 donor delivery and the CRISPR/Cas9 system delivered as ribonucleoproteins (RNPs

54 As, including miRNAs, can be targeted by the CRISPR/Cas9 system despite their lacking an open reading

55 The CRISPR/Cas9 system develops an adaptive immune resistanc

56 04 cells with PIK3R1 depletion introduced by CRISPR/Cas9 system displayed enhanced proliferation, mig

57 as a model, our results demonstrate that the CRISPR/Cas9 system disrupts latently integrated viral ge

58 Furthermore, the inducible CRISPR/Cas9 system dramatically decreased off-target eff

59 iciency and multiplexing capabilities of the CRISPR/Cas9 system enable a variety of otherwise challen

60 The CRISPR-Cas9 system enables global screens of gene functi

61 e (NAMPT) inhibitor, we demonstrate that the CRISPR/Cas9 system enables the generation of transient h

62 e multiplex gene-editing capabilities of the CRISPR/Cas9 system facilitate the generation of a single

63 The CRISPR/Cas9 system facilitates precise DNA modifications

64 larly interspaced short palindromic repeats (CRISPR)-Cas9 system for genome editing has greatly expan

65 s technology leverages the simplicity of the CRISPR-Cas9 system for multiplexed targeting of specific

66 discuss additional applications of modified CRISPR-Cas9 systems for use in T. gondii, such as regula

67 Recent applications of the CRISPR/Cas9 system for generating site-specific gene kno

68 osion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to hi

69 The CRISPR-Cas9 system has been adapted to generate autochth

70 Furthermore, a modified version of the CRISPR-Cas9 system has been developed to recruit heterol

71 The bacterial CRISPR-Cas9 system has been repurposed for genome engine

72 The bacterial CRISPR-Cas9 system has emerged as an effective tool for

73 RNA-guided genome editing with the CRISPR-Cas9 system has great potential for basic and cli

74 The repurposed CRISPR-Cas9 system has recently emerged as a revolutiona

75 The CRISPR-Cas9 system has recently emerged as a versatile t

76 The CRISPR-Cas9 system has revolutionized gene editing both

77 The CRISPR-Cas9 system has revolutionized the process of mak

78 Recently, the CRISPR-Cas9 system has triggered enormous interest in th

79 The CRISPR/Cas9 system has become an efficient gene editing

80 The CRISPR/Cas9 system has been employed to efficiently edit

81 The CRISPR/Cas9 system has been rapidly adopted for genome e

82 Recently, the CRISPR/Cas9 system has been used for efficient multiplex

83 The simplicity and precision of CRISPR/Cas9 system has brought in a new era of gene edit

84 The CRISPR/Cas9 system has emerged as an important tool in b

85 The CRISPR/Cas9 system has greatly improved our ability to e

86 The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for

87 In particular, the RNA-guided CRISPR/Cas9 system has recently been engineered to creat

88 CRISPR/Cas9 system has recently been repurposed to enabl

89 The CRISPR/Cas9 system has revolutionized mammalian somatic

90 Recently, the CRISPR/Cas9 system has suggested another way to selectiv

91 The CRISPR/Cas9 system has ushered in a new era of targeted

92 y interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) system has emerged in recent years as a hig

93 The unique properties of the CRISPR-Cas9 system have created new opportunities for hi

94 Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-gui

95 nce-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, cor

96 CRISPR/Cas9 systems have been used in a wide variety of

97 arly interspaced, short palindromic repeats (CRISPR)-Cas9 system, have enabled the recruitment of tra

98 Emerging technologies such as the CRISPR/Cas9 system, high-throughput mutant identificatio

99 e engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming

100 or HIF-2alpha knockout using the lentiviral CRISPR-Cas9 system in human established leukemic cells w

101 of target mutations after microinjecting the CRISPR/Cas9 system in metaphase II (MII) oocytes and zyg

102 and demonstrate the potential of transgenic CRISPR/Cas9 systems in antiviral research in other speci

103 The method was also applicable to the CRISPR/Cas9 system, including CRISPR/Cas9 mutant nickase

104 o overcome these shortcomings by integrating CRISPR/Cas9 system into a temperate phage genome, removi

105 uble knockout pigs using direct injection of CRISPR/Cas9 system into developing embryos.

106 ; transgenes or genome editing tools such as CRISPR/Cas9 systems) into embryos, for creating genetica

107 The CRISPR-Cas9 system is a powerful tool to edit eukaryotic

108 nclude that retroviral implementation of the CRISPR-Cas9 system is an efficient system for cellular p

109 The CRISPR-Cas9 system is commonly used in biomedical resear

110 The CRISPR/Cas9 system is a bacterial defense mechanism that

111 The CRISPR/Cas9 system is a powerful genome editing tool and

112 Overall, our results demonstrate that the CRISPR/Cas9 system is a powerful tool for genome manipul

113 The CRISPR/Cas9 system is a powerful tool for studying gene

114 The CRISPR/Cas9 system is a robust genome editing technology

115 CRISPR/Cas9 system is an emerging gene-editing technique

116 CRISPR/cas9 system is emerging as a novel genome editing

117 A unique capability of the CRISPR/Cas9 system is multiplex genome engineering by de

118 ndromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is emerging as a robust biotechnolog

119 CRISPRi, an adapted CRISPR-Cas9 system, is proposed to act as a strand-speci

120 The presence of the appropriate CRISPR/Cas9 system leads to an enhancement in the freque

121 ated via chromosomal translocation using the CRISPR/Cas9 system leads to focus formation in both OVCA

122 The genetic disruption of miR-277 using the CRISPR-Cas9 system led to failures in both lipid storage

123 s induced by miR-214 overexpression, and the CRISPR-Cas9 system-mediated LHX6 knockdown reversed the

124 However, transgenic CRISPR/Cas9 system-mediated viral genome targeting has n

125 Therefore, CRISPR/Cas9 systems need to be carefully designed to avo

126 Here we use the CRISPR-Cas9 system of genome editing to overcome this li

127 f anti-CRISPRs that specifically inhibit the CRISPR-Cas9 system of Neisseria meningitidis.

128 , we discuss the practical advantages of the CRISPR-Cas9 system over conventional and other nuclease-

129 The CRISPR-Cas9 system provides a versatile toolkit for geno

130 The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disru

131 ic repeats and the Cas9 RNA-guided nuclease (CRISPR/Cas9) system provides a new opportunity to create

132 Although the genome-wide specificities of CRISPR-Cas9 systems remain to be fully defined, the powe

133 This CRISPR/Cas9 system represents a highly effective and sca

134 ut was achieved by short hairpin RNAs or the CRISPR/Cas9 system, respectively, whereas enzymatic inhi

135 Deletions of TSA and TSB using the CRISPR/Cas9 system result in developmental defects in fl

136 ularly interspaced short palindromic repeat (CRISPR)-Cas9 system showed that PYCR2 loss of function l

137 Here we demonstrate that CRISPR/Cas9 systems targeting the human hemoglobin beta

138 These favorable properties have made the CRISPR-Cas9 system the technology of choice for sequence

139 By CRISPR/Cas9 system, the virulent genes of the newly isol

140 The advantages of the CRISPR-Cas9 system-the ease of RNA design for new target

141 acks chromosomal loci in live cells with the CRISPR-Cas9 system, then barcodes those loci by DNA sequ

142 larly interspaced short palindromic repeats (CRISPR)-Cas9 system to the mdx mouse model of DMD to rem

143 Applications of the CRISPR-Cas9 system to edit the genome have widely expand

144 In this study, we exploited the power of the CRISPR-Cas9 system to identify genes affecting the tumor

145 The adaptation of the CRISPR-Cas9 system to pooled library gene knockout scree

146 sfection was further demonstrated to deliver CRISPR-Cas9 systems to successfully modify and reprogram

147 We used the double-nicking CRISPR/Cas9 system to conduct site-specific mutagenesis

148 In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryo

149 Here we demonstrate the use of the CRISPR/Cas9 system to eliminate targeted chromosomes.

150 emonstrate for the first time the use of the CRISPR/Cas9 system to engineer the IL-8 gene, and tested

151 We herein applied the CRISPR/Cas9 system to generate mice with point mutations

152 Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null

153 We applied CRISPR/Cas9 system to generate Mstn knock-out (KO) rabbi

154 Here we adapt the CRISPR/Cas9 system to human cells for intracellular defe

155 ression, folding, and secretion, we used the CRISPR/Cas9 system to inactivate the collagen galactosyl

156 Here, we use the CRISPR/Cas9 system to restore the expression of the dyst

157 in vivo using viral-mediated delivery of the CRISPR/Cas9 system to somatic cells of adult animals.

158 tion zebrafish mutant lines by utilizing the CRISPR/Cas9 system to specifically disrupt the two alpha

159 , in order to understand the efficacy of the CRISPR/Cas9 system to target a gene within the genome of

160 e we report the utilization of the TALEN and CRISPR/Cas9 systems to induce targeted mutations and hom

161 The CRISPR-Cas9 system uses guide RNAs to direct the Cas9 en

162 esistance to exogenous genomic material, the CRISPR-Cas9 system utilizes a short single guide RNA (sg

163 Thus, the applicabilitiy of the CRISPR/Cas9 system was demonstrated for the first time f

164 The CRISPR/Cas9 system was recently developed as a powerful

165 oduced the known Pten KO phenotype using the CRISPR-Cas9 system we design viruses to target a gene th

166 ems, and most recently the highly accessible CRISPR/Cas9 systems, we now possess an unprecedented abi

167 The guide strands of the CRISPR/Cas9 systems were designed to have a range of mis

168 show that genomic sites could be cleaved by CRISPR/Cas9 systems when DNA sequences contain insertion