ライフサイエンスコーパス: CRLR

1 ity-modifying proteins (RAMPs) showed that a CRLR/RAMP receptor complex is required for intermedin si

2 tonin receptor-like receptor (CRLR), while a CRLR heterodimer with RAMP1 yields a calcitonin gene-rel

3 elial (HUVEC) cells, expressed both ADMR and CRLR.

4 IMD and CRLR, RAMP1, RAMP2 and RAMP3 were expressed in all cell

5 Co-expression of RAMP1 and CRLR reconstituted a CGRP receptor that was able to acti

6 -stimulated cAMP accumulation, CGRP binding, CRLR trafficking, and cell surface expression.

7 r in the regulation of peripheral tissues by CRLR and will allow development of new therapeutic agent

8 ing ligand specificity, we have co-expressed CRLR and RAMP proteins in the yeast Saccharomyces cerevi

9 e affinity of small molecule antagonists for CRLR/RAMP1.

10 C-terminal tail of RAMP 1 is unnecessary for CRLR function.

11 etermined the transcriptional start of human CRLR cDNA by 5'-RACE and cloned the proximal 5'-flanking

12 we demonstrated that co-expression of human CRLR with rat RAMP1 produced rat receptor pharmacology,

13 ragment contains the basal promoter of human CRLR, including potential TATA-boxes and several GC boxe

14 d by PCR contains the gene promoter of human CRLR.

15 igher affinity for the human receptor, human CRLR/human RAMP1, than for the rat receptor, rat CRLR/ra

16 artners of RAMP3 and determine their role in CRLR-RAMP3 trafficking.

17 n on NHERF-1 indicated that NHERF-1 inhibits CRLR/RAMP3 complex internalization by tethering the comp

18 , the ECD of RAMP 1 is sufficient for normal CRLR association and efficacy.

19 r (NSF) with the CRLR-RAMP3 complex, but not CRLR-RAMP1 or CRLR-RAMP2 complex, altered receptor traff

20 tic cancer cells expressed only ADMR but not CRLR.

21 e hypothesize that the Nt-domain of CRLR (Nt-CRLR) is an autonomously folded unit possessing a well-d

22 t rat uterine membrane in the presence of Nt-CRLR protein.

23 Far-UV CD and fluorescence spectra of Nt-CRLR showed characteristics of a folded protein.

24 The ability of Nt-CRLR to bind CGRP and AM independent of RAMPs was determ

25 We observe that Nt-CRLR inhibits (125)I-CGRP and (125)I-AM binding to rat u

26 together, our data provide evidence that Nt-CRLR is structured and further that a significant part o

27 the Nt-CRLR, we cloned and expressed the Nt-CRLR as a fusion protein in Escherichia coli.

28 uctural and functional information on the Nt-CRLR, we cloned and expressed the Nt-CRLR as a fusion pr

29 We hypothesize that the Nt-domain of CRLR (Nt-CRLR) is an autonomously folded unit possessing

30 Co-expression of CRLR with RAMP2 or RAMP3 resulted in a response with the

31 s in yeast, indicating that glycosylation of CRLR is not the prime determinant of ligand specificity.

32 ons in mammalian cells, the glycosylation of CRLR was not affected by the presence of RAMPs in yeast,

33 imultaneous transcriptional up-regulation of CRLR and its ligand adrenomedullin in endothelial cells

34 However, mechanisms of regulation of CRLR expression are still largely unknown.

35 ufficient to determine ligand specificity of CRLR.

36 hich exhibited a preferential stimulation of CRLR when co-expressed with RAMP1 and RAMP2 or RAMP3, re

37 he CRLR-RAMP3 complex, but not CRLR-RAMP1 or CRLR-RAMP2 complex, altered receptor trafficking to a re

38 By generating recombinant human/rat CRLR/RAMP1 receptors, we demonstrated that co-expression

39 /human RAMP1, than for the rat receptor, rat CRLR/rat RAMP1.

40 kawa cells express the putative ADM-receptor CRLR-RAMP2 the production and secretion of ADM with the

41 esence of NHERF-1, although the AM receptor (CRLR/RAMP3) undergoes desensitization, the internalizati

42 ession of calcitonin receptor-like receptor (CRLR) and an accessory protein called receptor activity-

43 combinant calcitonin receptor-like receptor (CRLR) and one of the three receptor activity-modifying p

44 tor named calcitonin receptor-like receptor (CRLR) has been identified, and in this study RCP co-immu

45 Calcitonin receptor-like receptor (CRLR) is a seven-transmembrane (7-TM) domain class B G p

46 on of the calcitonin receptor-like receptor (CRLR) with different members of the receptor activity mo

47 zation of calcitonin receptor-like receptor (CRLR) with receptor activity-modifying protein 1 (RAMP 1

48 or (GPCR) calcitonin receptor-like receptor (CRLR), and receptor activity modifying proteins (RAMPs)

49 ains, the calcitonin-receptor-like receptor (CRLR), can function as either a CGRP receptor or an adre

50 vely) and calcitonin receptor-like receptor (CRLR), while a CRLR heterodimer with RAMP1 yields a calc

51 receptor calcitonin receptor-like receptor (CRLR), with specificity being conferred by the receptor

52 ADMR) and calcitonin receptor-like receptor (CRLR).

53 RAMP3 and calcitonin receptor-like receptor (CRLR).

54 ts of at least three proteins: the receptor (CRLR), the chaperone protein (RAMP), and RCP that couple

55 sing the CRLR-RAMP3 complex and NHERF-1, the CRLR-RAMP complex desensitizes but is unable to internal

56 oscopy, we observed that in HEK293 cells the CRLR-RAMP complex undergoes agonist-stimulated desensiti

57 mal tubule cells endogenously expressing the CRLR-RAMP3 complex and NHERF-1, the CRLR-RAMP complex de

58 The internalization of the CRLR-RAMP complex was not affected by NHERF-1 when CRLR

59 abled agonist-induced internalization of the CRLR-RAMP complex.

60 hylmaleimide-sensitive factor (NSF) with the CRLR-RAMP3 complex, but not CRLR-RAMP1 or CRLR-RAMP2 com

61 hypertensive rats via interactions with the CRLR/RAMP receptor complexes.

62 P and adrenomedullin can both signal through CRLR, which has been previously shown to require a chape

63 peptide family capable of signaling through CRLR/RAMP receptor complexes provides an additional play

64 RAMPs are required to transport CRLR to the plasma membrane.

65 AMP complex was not affected by NHERF-1 when CRLR was co-expressed with RAMP1 or RAMP2.

66 examined for their ability to associate with CRLR to effect CGRP-stimulated cAMP accumulation, CGRP b

67 l RAMP 1 mutants were able to associate with CRLR with full efficacy for CGRP-stimulated cAMP accumul

68 Pre-treatment (4 days) of HAECs with CRLR or RAMP2, but not RAMP1 or RAMP3, siRNAs abolished

69 in this study RCP co-immunoprecipitated with CRLR indicating that these two proteins interact directl