ライフサイエンスコーパス: CTD kinase

1 he protein stability of Ctk1 (the major Ser2 CTD kinase).

2 recruits poised RNAPII PIC lacking the Kin28 CTD kinase.

3 AK components and thus may represent a novel CTD kinase.

4 s distinct from its ability to function as a CTD kinase.

5 ciency similar to Tax, also failed to bind a CTD kinase.

6 DK13 has been demonstrated to be a bona fide CTD kinase.

7 implicating it as the cyclin subunit of this CTD kinase.

8 a complex regulatory network governs Pol II CTD kinases.

9 ation by a relay of transcriptionally active CTD kinases.

10 se genes encode an Srb/mediator component, a CTD kinase, a CTD phosphatase, and a protein involved in

11 The Tat-, E1A- and VP16-associated CTD kinase activities detected in our assay also appear

12 rential requirement of Rad3 DNA helicase and CTD kinase activities in damage-specific incision versus

13 The CTD kinase activities that interact with E1A and VP16 ar

14 the contributions of TFIIH DNA helicase and CTD kinase activities to efficient promoter escape by RN

15 An inhibitor of CDK9 and CDK7 CTD kinase activities, TAF7 also binds to BRD4 and inhib

16 resulting in the inhibition of their Pol II CTD kinase activities.

17 iption through its well-described RNA Pol II CTD kinase activity and also through the Cdc2-activating

18 We show that CDK8 is associated with CTD kinase activity and that CDK8 co-fractionates with E

19 are sufficient for Mediator to enhance Kin28 CTD kinase activity and that Mediator enhances phosphory

20 rotein, Rsp5, is capable of stimulating this CTD kinase activity as well.

21 Regulation of CDK7-CTD kinase activity by p16(INK4A) thus may represent an

22 d suggest a mechanism for the enhancement of CTD kinase activity by the Mediator complex.

23 cell lysate demonstrates that Tat-associated CTD kinase activity elutes in two peaks.

24 indicates that the E1A- and VP16-associated CTD kinase activity has a molecular size of approximatel

25 DK13 purified from nuclear extracts displays CTD kinase activity in vitro, as anticipated.

26 d VP16 specifically interact with a cellular CTD kinase activity in vitro.

27 K12 purified from nuclear extracts manifests CTD kinase activity in vitro.

28 The CTD kinase activity is induced upon mitogenic stimulatio

29 This association greatly stimulates the CTD kinase activity of general transcription factor TFII

30 and that this phosphorylation stimulates the CTD kinase activity of Kin28p.

31 g kinase in TFIIH was unable to activate the CTD kinase activity of P-TEFb.

32 stimulated PBLs results in induction of the CTD kinase activity of the cyclin T1/CDK9 complex, which

33 ated to MO15, the catalytic component of the CTD kinase activity of the general transcription factor

34 ion in vivo, and recombinant Cak1 stimulates CTD kinase activity of the purified Bur1-Bur2 complex in

35 o-fractionates with E1A- and VP16-associated CTD kinase activity over several chromatography columns.

36 V irradiation or actinomycin D, which induce CTD kinase activity, and that UV inhibition can be rescu

37 abundance, which represses or activates CDK9 CTD kinase activity, respectively.

38 e levels of cyclin T1 protein and associated CTD kinase activity, suggests that the cyclin T1/CDK9 pa

39 cterized that Tat associated with a cellular CTD kinase activity, whereas Tax did not.

40 th of these cyclin C complexes retain potent CTD kinase activity.

41 responsible for the E1A- and VP16-associated CTD kinase activity.

42 sely, CDK9 phosphorylates BRD4 enhancing its CTD kinase activity.

43 of a high molecular weight complex that has CTD kinase activity.

44 y of TFIIH to activate transcription and its CTD kinase activity.

45 kinase activity but also by modulating CDK7-CTD kinase activity.

46 k1p), is not a component of TFIIH, and lacks CTD kinase activity.

47 XPD subunit, and a carboxyl-terminal domain (CTD) kinase activity catalyzed by its CDK7 subunit.

48 its RNA polymerase II COOH-terminal domain (CTD)-kinase activity, resulted in preferential inhibitio

49 yeast Cak1 kinase, in order to uncouple the CTD kinase and CAK activities of Mcs6, revealed an unant

50 Cyclin K is associated with potent CTD kinase and Cdk kinase (CAK) activity in vitro and co

51 ults identify Bur1 as a fourth S. cerevisiae CTD kinase and provide striking functional similarities

52 laries between activators with(out) a linked CTD kinase and regulated transcription by RNA polymerase

53 between viral transactivators and a cellular CTD kinase and suggests that at least two CTD kinases ma

54 purified calf thymus RNAP IIA with specific CTD kinases and used as substrates for FCP1.

55 with both CDK7 and CDK9 (putative RNA pol II CTD kinases) and that CDKN1C blocks their ability to pho

56 role for metazoan CDK7 as a broadly required CTD kinase, and as a CAK essential for cell cycle progre

57 ymerase II transcription machinery by MAPKs, CTD kinases, and phosphatases constitutes an essential m

58 emonstrate that metazoan CDK12 and CDK13 are CTD kinases, and that CDK12 is orthologous to yeast Ctk1

59 of the CTD; affect the Kin28, Bur1, or Ctk1 CTD kinases; and affect the CTD phosphatase Fcp1.

60 tro and in vivo under conditions where other CTD kinases are inactive.

61 olymerase (Pol) II carboxyl-terminal domain (CTD) kinase associated with transcription factor (TF) II

62 activity results in Kin28 being the primary CTD kinase at initiation.

63 al orthologs of the Saccharomyces cerevisiae CTD kinase Bur1/Bur2, a putative yeast P-TEFb.

64 d Set2, but in a manner stimulated by Pol II CTD kinase Cdk7/Kin28.

65 We report that three CTD kinases, CDK7, CDK9, and BRD4, engage in cross-talk,

66 Ser(2), Thr(4) phosphorylation requires the CTD kinase CDK9 and is evolutionarily conserved from yea

67 by a dominant-negative mutant of the pol II CTD kinase, CDK9, and by low concentrations of the CDK9

68 n T1, a regulatory subunit of the TAK/P-TEFb CTD kinase complex.

69 at the ability of p16(INK4A) to inhibit CDK7-CTD kinase contributes to the capacity to induce cell cy

70 About 60% of Tat-associated CTD kinase copurifies with CDK2 kinase activity and cont

71 among Nrd1p, the pol II CTD, Nab3p, and the CTD kinase CTDK-I.

72 eletion of the RNA pol II C-terminal domain (CTD) kinase Ctk1, or partial deletion of the CTD, result

73 sidual Ser2P in cells lacking the major Ser2 CTD kinase, CTK1.

74 s but does not require the RNA polymerase II CTD kinase Ctk1p.

75 NA polymerase II (Pol II) C-terminal domain (CTD) kinases cyclin-dependent kinase 7 (CDK7) and CDK9 a

76 e studied the properties of a Tat-associated CTD kinase derived from mitogenically stimulated human p

77 w that Ctk1, the serine 2 C-terminal domain (CTD) kinase for RNA polymerase II (RNAP II), regulates H

78 In contrast, TFIIH CTD kinase has a pronounced preference for RNAPII incorp

79 ition, we show that O-GlcNAc transferase and CTD kinase have different CTD repeat requirements for en

80 Two primary S2 position CTD kinases have been identified in higher eukaryotes: P

81 ion, we have analyzed the ability of a known CTD kinase, human Cdk8, to modulate HIV-1 LTR-driven gen

82 etic screen for suppressors of loss of yeast CTD kinase I (CTDK-I) function (by deletion of the catal

83 CTD kinase I (CTDK-I) of Saccharomyces cerevisiae is req

84 red the relationship between Srb10-Srb11 and CTD kinase I (CTDK-I), another member of the cdk-cyclin

85 ain (CTD) affinity column created with yeast CTD kinase I and the CTD of RNA polymerase II was used t

86 These results demonstrate that CTD kinase I modulates the elongation efficiency of RNA

87 templates, we examined the effects of yeast CTD kinase I or CTD kinase inhibitors on transcription a

88 itors, whereas both are greatly increased by CTD kinase I; in contrast, transcription initiation is m

89 onal specificity of phosphorylation by yeast CTD kinase-I (CTDK-I), an enzyme implicated in various n

90 ons demonstrate that c-Abl can function as a CTD kinase in vitro as well as in vivo.

91 thermore, direct competition between OGT and CTD kinase in vivo could generate multiple functionally

92 ily phosphorylated in front of the lesion by CTD kinases in the presence of ATP.

93 sactivators for the ability to interact with CTD kinases in vitro.

94 r elongation-phase C-terminal repeat domain (CTD) kinase in Saccharomyces cerevisiae, CTDK-I.

95 sphorylate the Rpb1 carboxy-terminal domain (CTD) kinase in vitro, it has no strong specificity withi

96 1-beta-d-ribofuranosylbenzimidazole (DRB), a CTD kinase inhibitor.

97 ion initiation complex and is blocked by the CTD-kinase inhibitor H8.

98 xamined the effects of yeast CTD kinase I or CTD kinase inhibitors on transcription and CTD phosphory

99 CTD kinase inhibitors, however, have little effect on in

100 NA polymerase II and treatment of cells with CTD kinase inhibitors, including DRB (5,6-dichloro-1-bet

101 However, CTD kinase inhibitors, such as 5,6-dichloro-1-beta-D-rib

102 In cultured cells treated with CTD kinase inhibitors, the dephosphorylation of RNAPII o

103 d CTD phosphorylation are greatly reduced by CTD kinase inhibitors, whereas both are greatly increase

104 In yeast, the major CTD kinase is a subunit of the general transcription fac

105 VP16, suggesting that the interaction with a CTD kinase is relevant for the transactivation function

106 s of the pol II C-terminal domain (CTD), the CTD kinase Kin28 and the holoenzyme subunit Srb2 all inh

107 Therefore, only the TFIIH-associated CTD kinase Kin28 appears necessary for proper capping en

108 (6-AU) and inactivation of pol II, TFIIE or CTD kinases Kin28 and Ctk1, this mark shifted to the 3'

109 ns between ESS1 and genes encoding the known CTD kinases (KIN28, CTK1, BUR1, and SRB10).

110 The CTD kinase, Kin28, is required for binding, and the CTD

111 ar CTD kinase and suggests that at least two CTD kinases may mediate responses to viral transactivato

112 sCTD extracted from cells mutated in several CTD kinases or phosphatases showed the expected changes

113 ex (SEC) with the RNA polymerase II (Pol II) CTD kinase P-TEFb.

114 s with cdk9 and cyclin T1, components of the CTD kinase P-TEFb.

115 shed by differential recruitment of the Ser2 CTD kinase, P-TEFb.

116 We sought to identify the CTD kinase responsible for capping enzyme targeting.

117 snRNA promoters recruit factors including a CTD kinase(s) whose snRNA-specific phosphorylation patte

118 not stimulate the activity of several other CTD kinases, suggesting that the specific enhancement of

119 Previously, we reported that a cellular CTD kinase, TAK, is targeted by the human immunodeficien

120 ished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation

121 CDK12 (CG7597) is a transcription-associated CTD kinase, the ortholog of yCtk1.

122 on factor-b) (CycT1:CDK9) C-terminal domain (CTD) kinase to the HIV-1 promoter.

123 by recruitment of carboxyl-terminal domain (CTD) kinases to the HIV-1 promoter.

124 CTD kinases with different phosphoryl acceptor specifici

125 Furthermore, recruitment of FACT requires CTD kinases, yet FACT is dispensable for p21(CIP1) expre