ライフサイエンスコーパス: CTP synthetase

1 n kinase C also phosphorylates and activates CTP synthetase.

2 the nucleotide-dependent oligomerization of CTP synthetase.

3 amination of intrarickettsial UTP to CTP by CTP synthetase.

4 The CTP synthetase 1 activity of the T455A mutant enzyme was

5 phosphorylation at Ser(462) stimulates human CTP synthetase 1 activity, whereas phosphorylation at Th

6 utant enzyme exhibited a 2-fold reduction in CTP synthetase 1 activity, whereas the purified T455A mu

7 esidue, was accompanied by a 50% decrease in CTP synthetase 1 activity.

8 yme was accompanied by a 95-fold increase in CTP synthetase 1 activity.

9 utant enzyme exhibited a 2-fold elevation in CTP synthetase 1 activity.

10 dent, and dependent on the concentrations of CTP synthetase 1 and ATP.

11 ses (2-fold) in the phosphorylation of human CTP synthetase 1 and the cellular level of CTP.

12 Using purified CTP synthetase 1 as a substrate, protein kinase A activi

13 Using purified Escherichia coli-expressed CTP synthetase 1 as a substrate, protein kinase C activi

14 Phosphorylation of human CTP synthetase 1 by mammalian protein kinase C was exami

15 e phosphorylation of the human CTPS1-encoded CTP synthetase 1 by protein kinase A.

16 and phosphopeptide mapping analyses of human CTP synthetase 1 isolated from (32)P(i)-labeled cells re

17 mapping analysis of S. cerevisiae-expressed CTP synthetase 1 mutant enzymes phosphorylated with mamm

18 inase C resulted in a 50% increase for human CTP synthetase 1 phosphorylation in the Saccharomyces ce

19 tions resulted in decreases in the extent of CTP synthetase 1 phosphorylation that occurred in vivo.

20 Using human CTP synthetase 1 purified from Escherichia coli as a sub

21 caused a 44% decrease in the amount of human CTP synthetase 1 that was phosphorylated in S. cerevisia

22 Human CTP synthetase 1 was also phosphorylated by mammalian pr

23 A Thr(455) to Ala (T455A) mutation in CTP synthetase 1 was constructed by site-directed mutage

24 CTP synthetase 1 was expressed and purified from a Sacch

25 g and phosphoamino acid analyses showed that CTP synthetase 1 was phosphorylated on multiple serine a

26 d dependent on the concentrations of ATP and CTP synthetase 1.

27 that enzyme tetramerization was required for CTP synthetase activity and that enzyme phosphorylation

28 These data indicated that the regulation of CTP synthetase activity by CTP plays an important role i

29 an 80% decrease in the stimulatory effect on CTP synthetase activity by protein kinase C.

30 In this work we examined the regulation of CTP synthetase activity by S. cerevisiae protein kinase

31 bolism of the host cell: 1) the elevation of CTP synthetase activity in an ndk mutant, in which the s

32 In contrast, CTP synthetase activity in cells bearing the S330A mutan

33 The CTP synthetase activity in extracts from cells bearing t

34 that the dUTP-dependent synthesis of dCTP by CTP synthetase activity may be physiologically relevant.

35 A and C together resulted in an increase in CTP synthetase activity that was slightly greater than t

36 CTP synthetase proteins, the measurement of CTP synthetase activity, and the synthesis of CTP in viv

37 CTP, a potent inhibitor of CTP synthetase activity, did not inhibit the ATP/UTP-dep

38 t affect the mechanism by which CTP inhibits CTP synthetase activity.

39 iae ura7Delta ura8Delta mutant lacking yeast CTP synthetase activity.

40 ura7Delta ura8Delta double mutant that lacks CTP synthetase activity.

41 pe of the ura7Delta ura8Delta mutant lacking CTP synthetase activity.

42 ally in a ura7 ura8 double mutant that lacks CTP synthetase activity.

43 this site played a role in the regulation of CTP synthetase activity.

44 This correlated with the inactivation of CTP synthetase activity.

45 n correlated with the partial restoration of CTP synthetase activity.

46 In vitro phosphorylated CTP synthetase also exhibited a decrease in its positive

47 aused increases in the apparent Km values of CTP synthetase and ATP of 20-and 2-fold, respectively, i

48 r maximum phosphorylation and stimulation of CTP synthetase and that the phosphorylation of this site

49 dUTP promoted the tetramerization of CTP synthetase, and the extent of enzyme tetramerization

50 e pairs generated during kinetic analysis of CTP synthetase are greatly simplified, further facilitat

51 activity was dose- and time-dependent using CTP synthetase as a substrate.

52 In vitro, protein kinase A phosphorylated CTP synthetase at a serine residue with a stoichiometry

53 vitro data indicated that phosphorylation of CTP synthetase at Ser330 affected the phosphorylation of

54 The phosphorylation of CTP synthetase at Ser36, Ser330, Ser354, and Ser454 resi

55 litated the UTP-dependent tetramerization of CTP synthetase by a mechanism that involved the ATP-depe

56 M) the phosphorylation of purified wild-type CTP synthetase by protein kinase A.

57 nted for 10% of the total phosphorylation of CTP synthetase by protein kinase C.

58 ragine synthetase, cytidine 5'-triphosphate (CTP) synthetase, carbamoyl phosphate synthetase, and pho

59 The S424A mutation in CTP synthetase caused a 50% decrease in the phosphorylat

60 CTP Synthetase (CtpS) is a universally conserved and ess

61 Phosphorylation of CTP synthetase did not have a significant effect on the

62 ramerization of purified native URA7-encoded CTP synthetase (EC 6.3.4.2, UTP: ammonia ligase (ADP-for

63 orylation and regulation of the URA7-encoded CTP synthetase (EC 6.3.4.2, UTP:ammonia ligase (ADP-form

64 CTP synthetase (EC 6.3.4.2, UTP:ammonia ligase (ADP-form

65 CTP synthetase (EC 6.3.4.2, UTP:ammonia ligase (ADP-form

66 CTP synthetase (EC 6.3.4.2, UTP:ammonia ligase (ADP-form

67 The URA7-encoded CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-form

68 CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-form

69 ylcholine in cells carrying the S424A mutant CTP synthetase enzyme was reduced by 48, 32, and 46%, re

70 Using a purified S424A mutant CTP synthetase enzyme, we examined the effect of Ser424

71 ession of the CTPS1- and CTPS2-encoded human CTP synthetase enzymes in the ura7Delta ura8Delta mutant

72 ptide maps of the wild type and S424A mutant CTP synthetase enzymes phosphorylated by protein kinases

73 ssing the E161K mutation in the URA7-encoded CTP synthetase exhibited an increase (1.5-fold) in the u

74 CTP synthetase existed as a dimer in the absence of ATP

75 In this work we showed that the human CTP synthetase genes, CTPS1 and CTPS2, were functional i

76 The S424A mutant CTP synthetase had reduced Vmax and elevated Km values f

77 375575), a specific inhibitor of the enzyme CTP synthetase, induces irreversible growth arrest and s

78 iments with cyclopentenyl cytosine (CPEC), a CTP synthetase inhibitor and cytosine, suggest that the

79 CTP synthetase is an essential enzyme that generates the

80 In vivo, the phosphorylation of CTP synthetase is mediated by the RAS/cAMP pathway.

81 The Saccharomyces cerevisiae URA7-encoded CTP synthetase is phosphorylated and stimulated by prote

82 The Saccharomyces cerevisiae URA7-encoded CTP synthetase is phosphorylated and stimulated by prote

83 kinase A sequence motif in the URA7-encoded CTP synthetase is the target site for protein kinase A.

84 The B. subtilis pyrG gene, which encodes CTP synthetase, is located far from the pyrimidine biosy

85 e Bacillus subtilis pyrG gene, which encodes CTP synthetase, is repressed by cytidine nucleotides.

86 d regulation of the pyrG gene, which encodes CTP synthetase, occurs through an attenuation mechanism

87 In vitro, Pkc1p phosphorylated purified CTP synthetase on serine and threonine residues, which r

88 evisiae was useful for the analysis of human CTP synthetase phosphorylation.

89 aturating concentrations of ATP and UTP, the CTP synthetase protein existed as a tetramer.

90 a mutant was shown by immunoblot analysis of CTP synthetase proteins, the measurement of CTP syntheta

91 Phosphorylation of CTP synthetase resulted in a decrease in the enzyme's se

92 consistent with one phosphorylation site per CTP synthetase subunit.

93 A CTP synthetase synthetic peptide (SLGRKDSHSA) containing

94 A CTP synthetase synthetic peptide (SLGRKDSHSA) containing

95 lite prodrug 3-deazauridine (3DUrd) inhibits CTP synthetase upon intracellular conversion to its trip

96 scription factor Gln3 or inactivation of the CTP synthetase Ura7 both resulted in the activation of t

97 In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesiz

98 s cooperative (n = 1.8) and the Km value for CTP synthetase was 73 nM.

99 e dependence of protein kinase A activity on CTP synthetase was cooperative (n = 1.8) and the Km valu

100 The tetramerization of CTP synthetase was dependent on UTP and Mg2+ ions.

101 Phosphorylated CTP synthetase was less sensitive to product inhibition

102 PKC1 gene indicated that phosphorylation of CTP synthetase was mediated by Pkc1p in vivo.

103 iments indicated that the phosphorylation of CTP synthetase was mediated by the RAS/cAMP pathway in v

104 Purified S424A mutant CTP synthetase was not phosphorylated and stimulated by

105 ted that the protein kinase A target site in CTP synthetase was Ser424 and that the phosphorylation o

106 Glu161 in the URA7-encoded and URA8-encoded CTP synthetases was identified as being involved in the

107 nstants for ATP and UTP for the S424A mutant CTP synthetase were 4.2- and 2.9-fold lower, respectivel

108 (S36A, S330A, S354A, and S454A) mutations in CTP synthetase were constructed by site-directed mutagen

109 e E161K mutant URA7-encoded and URA8-encoded CTP synthetases were less sensitive to CTP product inhib

110 ined in a protein kinase C sequence motif in CTP synthetase, were target sites for the kinase.

111 CTP synthetase with a Ser424 --> Ala (S424A) mutation wa

112 Dephosphorylation of native CTP synthetase with alkaline phosphatase prevented the n

113 Phosphorylation of the purified native CTP synthetase with protein kinase A and protein kinase

114 Phosphorylation of CTP synthetase with protein kinase A resulted in the sti

115 Phosphorylation of CTP synthetase with protein kinases A and C together res