ライフサイエンスコーパス: CaMV

1 CaMV infection altered the expression of the herbicide t

2 CaMV replicates by reverse transcription of a 358 RNA th

3 S enhancer or upstream of a truncated (-209) CaMV 35S promoter, respectively.

4 nsuccessful when a constitutive promoter (2x CaMV 35S) was used in the plasmid construction, but seve

5 ronidase gene terminated with either the 35S CaMV 3' untranslated sequence (UTR) or a cis-acting ribo

6 of the tomato HypSys precursor under the 35S CaMV promoter show that the transgenic plants regulate p

7 dopsis thaliana under the control of the 35S CaMV promoter.

8 The lethal gene used here is a CaMV 35S-barnase gene with an intron in the coding seque

9 linked uidA gene when placed downstream of a CaMV 35S enhancer or upstream of a truncated (-209) CaMV

10 e SSE or middle region upstream of p109 or a CaMV 35S basal promoter (-64 to +6) were fused to gus.

11 irus (CaMV) 35S promoter-TTS2 transgene or a CaMV 35S-promoter-NAG1 (NAG1 = Nicotiana tabacum Agamous

12 genetic complementation experiments using a CaMV 35S::ZmF3'H1 promoter-gene construct established th

13 tion in the 2-hybrid system between MPI7 and CaMV MP mutants correlated with the infectivity of the m

14 chlorosis and stunting in P6-transgenic and CaMV-infected plants are dependent on interactions betwe

15 us particle acquisition by aphid vectors and CaMV transmission.

16 Because CaMV virions accumulate preferentially in P6 IBs, we hyp

17 o inhibits the formation of local lesions by CaMV in Nicotiana edwardsonii leaves.

18 used here act differently than the complete CaMV 35S promoter.

19 ion of a BRL1 cDNA, driven by a constitutive CaMV 35S promoter, recapitulates the bri1-5 suppression

20 MAP65-2 were expressed behind a constitutive CaMV 35S promoter, suggesting a level of post-transcript

21 scripts when transcribed from a constitutive CaMV promoter.

22 lgaris under the control of the constitutive CaMV 35S promoter and Nos terminator via Agrobacterium r

23 ted region (UTR), driven by the constitutive CaMV 35S promoter in Medicago sativa (alfalfa) and Nicot

24 RLK genes under control of the constitutive CaMV 35S promoter or a steroid-inducible Ga14 promoter.

25 ase (OxO) gene regulated by the constitutive CaMV 35S promoter was expressed in a hybrid poplar clone

26 esized that P6 IBs have a role in delivering CaMV virions to the plasmodesmata.

27 f cytoplasmic inclusion bodies formed during CaMV infection.

28 tutive promoters, either the doubly enhanced CaMV 35S promoter or the chimaeric 'Super-Promoter'.

29 rly endosomes, for tubule formation, and for CaMV infection.

30 endent of Box I when fused to a heterologous CaMV 35S minimal promoter and introduced to transgenic r

31 We conclude that infectious CaMV replicons can be used to carry a variety of element

32 owever, in plants inoculated with infectious CaMV DNA rather than virus particles, the onset of syste

33 s found immediately adjacent to the inserted CaMV 35S enhancers, at distances ranging from 380 bp to

34 ly recombinogenic' region of the full-length CaMV RNA that has been shown to promote viral recombinat

35 A longer CaMV 35S minimal promoter than was used in the original

36 n of phas 3' MAR or coding sequences lowered CaMV 35S enhancer driven GUS expression from the phas ba

37 ere placed upstream from a -46 to +1 minimal CaMV 35S promoter-luciferase reporter gene and reporter

38 ance pollen-specific expression of a minimal CaMV 35S promoter.

39 d C, each sufficient to activate the minimal CaMV 35S promoter in a pollen-specific manner.

40 (CaMV) 35S promoter required the deletion of CaMV 5' leader and polylinker sequences from the constru

41 on, thereby restricting the establishment of CaMV infection.

42 a polypurine tract specifying initiation of CaMV plus strand DNA synthesis was inserted into a 35S e

43 may be involved in cell-to-cell movement of CaMV as an intermediate that is transported through plas

44 n intracellular and cell-to-cell movement of CaMV.

45 The presence of CaMV and polylinker sequences at the 5' end of the PetE

46 razil nut was placed under the regulation of CaMV 35S promoter and nopaline synthase terminator and i

47 inase complexes as important host targets of CaMV for transcriptional activation of viral genes and c

48 In plants, the same CaMV sequence has been shown to have an essential role i

49 Altogether, these results demonstrate that CaMV MP traffics in the endocytic pathway and that virus

50 We report here that CaMV 35S promoter driven overexpression of the Arabidops

51 Our results suggest that CaMV can perceive aphid vectors, either directly or indi

52 The CaMV 35S promoter is the most commonly used promoter for

53 The CaMV movement protein (MP) was used in a yeast 2-hybrid

54 The CaMV replicon carrying the pds gene fragment produced un

55 modesmata-Localized Protein1 (PDLP1) and the CaMV movement protein (MP).

56 icating an interaction in planta between the CaMV MP and MPI7.

57 FP) from Aequoria victoria was driven by the CaMV 35S promoter (line mGFP3).

58 Expression of a reporter gene driven by the CaMV 35S promoter is markedly reduced in the cdkc;2 and

59 ds for the bar gene expression driven by the CaMV 35S promoter or by the rice actin 1 promoter.

60 lemented by KAT2 or KAT5 cDNAs driven by the CaMV 35S promoter, showing that these enzymes are functi

61 with an artificial intron and driven by the CaMV 35S promoter, transient GUS expression was dramatic

62 A in the antisense orientation driven by the CaMV 35S promoter.

63 In at least one case, the CaMV 35S enhancers led primarily to an enhancement of th

64 A in transgenic tomato plants containing the CaMV 35S promoter driving the expression of the GAST1 tr

65 detected in nodules of plants containing the CaMV 35S promoter-beta-glucuronidase gene construct, sug

66 (-46 bp) promoter fragment derived from the CaMV 35S gene, no induction by MeJA treatment was detect

67 thought to be sites of translation from the CaMV 35S polycistronic RNA intermediate, the precise rol

68 Expression of MAX2 from the CaMV 35S promoter complements the max2 mutant, does not

69 In contrast, when expressed from the CaMV 35S promoter in transgenic plants carrying a pOp-GU

70 full-length or truncated petH cDNA from the CaMV 35S promoter in wild-type Arabidopsis.

71 y flowering, because overexpression from the CaMV 35S promoter of the co-3 allele, that has a mutatio

72 Expression of a MYC-146 cDNA from the CaMV 35S promoter was unable to complement the anthocyan

73 HEC genes are ectopically expressed from the CaMV 35S promoter, some of the resulting transgenic plan

74 When LhG4 was expressed from the CaMV 35S promoter, the spatial and quantitative expressi

75 l silencing of transgenes expressed from the CaMV 35S promoter.

76 ecipitation and colocalization assays in the CaMV host Nicotiana benthamiana.

77 ate, the precise role of these bodies in the CaMV infection cycle remains unclear.

78 ese areas with other sequences including the CaMV 35S promoter failed to replace activity.

79 have become incorporated end to end into the CaMV genome.

80 Intriguingly, the CaMV-induced virus factory inclusions seem to protect ag

81 -expressing UGT84B1 under the control of the CaMV 35S promoter have been constructed and their phenot

82 expressed CmGA20ox1 under the control of the CaMV 35S promoter in Solanum dulcamara to assess the use

83 d the expression of mfs under control of the CaMV 35S promoter in transformed peppermint plants.

84 haliana under transcriptional control of the CaMV 35S promoter of the APS reductase from Pseudomonas

85 s in transgenic tobacco under control of the CaMV 35S promoter supports the view that they can regula

86 omic sequence, including the TATA box of the CaMV 35S promoter, acted as a recombination hotspot.

87 ssed constitutively under the control of the CaMV 35S promoter, both TaLsi1 and OsLsi1 were expressed

88 an AAE14 transgene under the control of the CaMV 35S promoter, led to full complementation of the mu

89 rditis IRES element under the control of the CaMV 35S promoter.

90 imerization domains under the control of the CaMV 35S promoter.

91 at interact with the N-terminal third of the CaMV MP.

92 ng part of the gene under the control of the CaMV promoter, both the transgenic alfalfa and Arabidops

93 exceeded those obtained with pOp/LhG4 or the CaMV 35S promoter but without increased uninduced activi

94 sed to the virion sense promoter (Pv) or the CaMV 35S promoter, to suspension culture cells and immat

95 omoter showed much greater activity than the CaMV 35S promoter.

96 tive ectopic expression, suggesting that the CaMV 35S enhancers used here act differently than the co

97 the yeast genome sequence revealed that the CaMV element had sequence similarity with the R region o

98 threonine dehydratase/deaminase (TD), to the CaMV 35S promoter and transformed these constructs into

99 n-helix motif that specifically binds to the CaMV 35S promoter.

100 nt MAX2, lacking the F-box domain, under the CaMV 35S promoter does not complement max2, and dominant

101 stance genes in transgenic tobacco using the CaMV 35S promoter.

102 Leaves of alfalfa plants with the CaMV 35S promoter-GS1 gene showed high levels of accumul

103 ;4/CYCT1;5 results in complete resistance to CaMV as well as altered leaf and flower growth, trichome

104 ;5 double mutants are extremely resistant to CaMV.

105 We analyzed the response to CaMV infection of a transgenic oilseed rape line contain

106 ies and ethylene in signaling in response to CaMV infection, but suggest that salicylic acid does not

107 tants, also showed reduced susceptibility to CaMV, whereas in NahG transgenics, virus levels were sim

108 Oilseed rape is susceptible to CaMV, but plants recover from infection.

109 a model in which P6 IBs function to transfer CaMV virions directly to MP at the plasmodesmata.

110 Transgenic CaMV 35S::RAN1 plants showed constitutive expression of

111 ted in the least recovery of the transgenic (CaMV 35S promoter) and taxon-specific (zein) target DNA

112 nd vegetative development was examined using CaMV 35STag1-GUS constructs.

113 enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants.

114 consisting of the cauliflower mosaic virus (CaMV) 35S promoter driving a cytosolic isoform of GS (GS

115 the control of the cauliflower mosaic virus (CaMV) 35S promoter exhibited delayed root emergence, red

116 Frequently, the cauliflower mosaic virus (CaMV) 35S promoter is used to drive expression of the he

117 cts containing the cauliflower mosaic virus (CaMV) 35S promoter required the deletion of CaMV 5' lead

118 , two utilized the cauliflower mosaic virus (CaMV) 35S promoter with duplicated enhancer, and four ut

119 tron (AGI) and the Cauliflower Mosaic Virus (CaMV) 35S promoter, respectively, in the presence and ab

120 t express either a cauliflower mosaic virus (CaMV) 35S promoter-TTS2 transgene or a CaMV 35S-promoter

121 xpressed under the cauliflower mosaic virus (CaMV) 35S promoter.

122 rary driven by the cauliflower mosaic virus (CaMV) 35S promoter.

123 ls compared to the cauliflower mosaic virus (CaMV) 35S promoter.

124 y the constitutive cauliflower mosaic virus (CaMV) double 35S promoter.

125 Gene I of cauliflower mosaic virus (CaMV) encodes a protein that is required for virus movem

126 enic region of the Cauliflower mosaic virus (CaMV) genome for promoter activity in baker's yeast (Sac

127 Cauliflower mosaic virus (CaMV) is a double-stranded DNA (dsDNA) pararetrovirus ca

128 VI product (P6) of Cauliflower mosaic virus (CaMV) is a multifunctional protein known to be a major c

129 Cauliflower mosaic virus (CaMV) is aphid-transmitted, with the virus being taken u

130 The P6 protein of Cauliflower mosaic virus (CaMV) is responsible for the formation of inclusion bodi

131 Here, we show that cauliflower mosaic virus (CaMV) MP contains three tyrosine-based sorting signals t

132 rds development of cauliflower mosaic virus (CaMV) replicons for propagation of functional elements d

133 uring infection by Cauliflower mosaic virus (CaMV), a compatible pathogen of Arabidopsis (Arabidopsis

134 ble interaction of cauliflower mosaic virus (CaMV), a double-stranded DNA pararetrovirus, with the mo

135 tom determinant of cauliflower mosaic virus (CaMV), and transgene-mediated expression in Arabidopsis

136 g virus (TVCV) and cauliflower mosaic virus (CaMV).

137 in infection with Cauliflower mosaic virus (CaMV).

138 5S transgenes from cauliflower mosaic virus (CaMV).

139 becoming infected following inoculation with CaMV was 40% that of wild-type, although in plants that

140 n had previously been shown to interact with CaMV MP, we investigated whether P6 I-LBs might also be

141 olumbia and No-0) that were transformed with CaMV 35S-Tag1-GUS DNA.