ライフサイエンスコーパス: Ci

1 Ci protein lacking this region is still able to interact

2 Ci resides in the cytoplasm in a latent form, where Hh r

3 Ci uses multiple Ser/Thr (S/T)-rich motifs that bind HIB

4 Ci(155) functions as a transcriptional activator of a nu

5 Ci-Dll-B is located in a convergently transcribed bigene

6 Ci-MRF is the sole myogenic regulatory factor (MRF) of t

7 Ci-Slc26aalpha acts as an anion transporter, mediating t

8 Ci-Slc26aalpha is indispensable for lumen formation and

9 Ci-VSD can be reconstituted into liposomes of various co

10 ecific activity of 40-111 GBq/mumol (1.1-3.0 Ci/mumol).

11 (+/-SD) was 7 +/- 2 GBq/mumol (0.19 +/- 0.05 Ci/mumol), and radiochemical purity was greater than 99%

12 Starting with 29.6-37 GBq (0.8-1 Ci) of (18)F-fluoride, more than 7.4 GBq (>200 mCi) of (

13 activities of at least 10 microCi/microg (1 Ci = 37 GBq) were routinely achieved, and no additional

14 ific activity (2.1 GBq/mmol, 58 mCi/mmol) (1 Ci = 37 GBq) and no detectable dilution of label from en

15 g nearly a gram of antibody with 481 GBq (13 Ci) of (131)I during a single 30-min reaction run.

16 onal distribution is found: between delta(13)Ci = -11 and -53 per thousand.

17 al abundance distribution of (13)C (delta(13)Ci) within the molecule with better than 1 per thousand

18 ific activity of 8.0 x 10(7) MBq/mmol (2,166 Ci/mmol) and a radiochemical purity greater than 98%.

19 /- 3% and a specific activity of 2.2 +/- 0.2 Ci/micromol.

20 2%, and specific activity was 44.4 GBq (1.2 Ci)/mumol.

21 P) and specific activity (SA) of 2.6 +/- 1.2 Ci/mumol (n = 13) at end of synthesis (EOS).

22 l [(3)H]2-AG with a specific activity of 200 Ci/mmol for enzyme assays, metabolic studies, and tissue

23 urity at high specific activity (>111 GBq [3 Ci]/mumol).

24 at the notochord expression of Ciona Tbx2/3 (Ci-Tbx2/3) requires Ci-Bra, and identified a Ci-Tbx2/3 n

25 fic activity of 18,500-48,100 GBq (500-1,300 Ci)/mmol.

26 )H]PSB-13253, with a specific activity of 36 Ci (1.33 TBq)/mmol.

27 c activities of 666 +/- 51.8 GBq (18 +/- 1.4 Ci)/mumol at the end of synthesis and was validated for

28 on) and a specific activity of 39.0 +/- 12.4 Ci mumol(-1) within 77 +/- 4 min.

29 n beam current, producing up to 348 GBq (9.4 Ci) of (99m)Tc.

30 Sch225336 has high specific activity (>1,400 Ci/mmol) and affinity for hCB2 (65 pm).

31 ]propylbicycloorthobenzoate ([(3)H]EBOB) (46 Ci/mmol), illustrating the use of AMS for characterizing

32 ru-2,6-P(2) having a specific activity of 50 Ci/mmol was obtained in yields averaging 35%.

33 suggest that a center receiving 1.85 TBq (50 Ci) of (99)Mo once every 4 d can provide 1.48-3.33 TBq (

34 injectable [(18)F]7 from up to 285 GBq (7.7 Ci) of [(18)F]fluoride in 50 min (uncorrected radiochemi

35 pound was 853 +/- 29.6 GBq/mumol (23 +/- 0.8 Ci/mumol).

36 e every 4 d can provide 1.48-3.33 TBq (40-90 Ci) of (99m)Tc daily.

37 This complex controls the level of TRA-1A, a Ci/Gli homolog and master regulator of sex determination

38 in states of the photosynthetic apparatus: a Ci-limited state and a light-limited state.

39 Ci-Tbx2/3) requires Ci-Bra, and identified a Ci-Tbx2/3 notochord CRM that necessitates multiple Ci-Br

40 of aPKC is up-regulated by Hh signaling in a Ci-dependent manner.

41 We also provide evidence that PP2A is a Ci phosphatase.

42 We used a global dataset of A-Ci curves (564 species from 46 field sites, covering a r

43 Estimating this parameter using A-Ci curves (net photosynthesis, A, vs intercellular CO2 c

44 rate vs internal CO2 concentration curves (A/Ci ), and relationships with foliar nitrogen (N) and P c

45 released by CAH3 dehydration of accumulated Ci.

46 nvergent total synthesis of (-)-nahuoic acid Ci(Bii) (3), a novel cis-decalin polyketide, has been ac

47 family gene, and diverse mechanisms activate Ci-Mrf Here, we show that myogenesis in the atrial sipho

48 itro by the muscle-specific T-box activators Ci-Tbx6b and Ci-Tbx6c.

49 in HLA3 is proposed to be involved in active Ci uptake across the plasma membrane.

50 upon the transcription factors Ci-ets1/2 and Ci-mesp to generate cardiac progenitors.

51 d, resulted in increased Ci accumulation and Ci-dependent photosynthetic O2 evolution specifically in

52 decreases in photosynthetic Ci affinity and Ci uptake, the combination of nearly complete knockdown

53 e orthologous transcription factors Gli2 and Ci, respectively.

54 malian kinesin Kif7 can also direct Gli3 and Ci processing in fly, underscoring a fundamental conserv

55 We provide evidence that both HIB and Ci form dimers/oligomers and engage in multivalent inter

56 or voltage-sensing domain proteins (Hv1 and Ci-VSP) into eukaryotic voltage-activated potassium chan

57 lose-1,5-bisphosphate, phosphoglycerate, and Ci pools when grown under comparable conditions.

58 55 contributes to both Ci-155 processing and Ci-155 silencing in the absence of Hh.

59 influence Hh signaling by regulating Smo and Ci, respectively.

60 late Hh signaling by phosphorylating Smo and Ci; however, the phosphatase(s) involved remain obscured

61 nterfering with the formation of Ci-Sufu and Ci-Cos2-kinase complexes that normally inhibit Ci activi

62 uscle-specific T-box activators Ci-Tbx6b and Ci-Tbx6c.

63 H(+) 'shuttle' conductance (GSH) in VGCs and Ci VSP, and we now report that R1H is sufficient to reco

64 to the intracellular components to attenuate Ci cleavage.

65 e have obtained evidence that Wdb attenuates Ci processing probably by dephosphorylating Ci.

66 O(3)(-) is the predominant form of available Ci.

67 Here we show that Sufu can bind Ci/Gli through a C-terminal Sufu-interacting site (SIC)

68 he CORD domain of Ci-155 contributes to both Ci-155 processing and Ci-155 silencing in the absence of

69 onal binding sites for both Ciona Brachyury (Ci-Bra) and FoxA (Ci-FoxA-a).

70 We found that Ciona Brachyury (Ci-Bra) controls most of its targets directly, through n

71 ecific transcription factor Ciona Brachyury (Ci-Bra).

72 the known enhancer that regulates Brachyury (Ci-Bra), a key determinant of notochord specification.

73 34 potential noncoding RNAs was affected by Ci supply, although most of these molecules were not reg

74 gh the same T-box sites that are utilized by Ci-Bra in the notochord, which are also bound in vitro b

75 wth, photosynthetic Ci affinity, and [(14)C]-Ci uptake in very low CO(2) conditions following RNA int

76 anobacterium to changes in inorganic carbon (Ci) availability.

77 under different light and inorganic carbon (Ci) conditions as well as under genetic perturbations.

78 respiration in response to inorganic carbon (Ci) limitation.

79 sition and assimilation of inorganic carbon (Ci) represents the largest flux of inorganic matter in p

80 Active inorganic carbon (Ci) uptake, Rubisco sequestration and interconversion be

81 hydrases (CAs), and active inorganic carbon (Ci) uptake.

82 tii acclimates to limiting inorganic carbon (Ci), either low-CO2 (L-CO2; air level; approximately 0.0

83 cyanobacteria with similar inorganic carbon (Ci; as CO2 and HCO3-) transporter systems.

84 ncluding identification of inorganic carbon (Ci; CO(2) and HCO(3)(-)) transporters; however, specific

85 ctly to the unliganded asymmetric "carrier" (Ci) distribution.

86 transport and a role for LCIB in chloroplast Ci accumulation.

87 requires phosphorylation of full-length Ci (Ci-155) by protein kinase A (PKA), casein kinase 1 (CK1)

88 cited scientists, using the total citations Ci of each scientist as his/her reputation measure.

89 observed that Li is related with the clicks (Ci) to news stories and the age (Ti) of stories.

90 tomatal conductance (gs), intercellular CO2 (Ci), chlorophyll (Chl) content in WT plants as compared

91 second, through increased leaf internal CO2 (Ci ) and decreased stomatal limitations (Slim ).

92 s-derived reduction in intercellular [CO2 ] (Ci ) remains controversial and genetic analyses are need

93 esis, A, vs intercellular CO2 concentration, Ci ) is laborious, which limits availability of Vcmax da

94 urbation of phosphoinositide concentrations, Ci-VSPTEN will be useful for probing the role and specif

95 oxysome types showed a response to cytosolic Ci, which is of higher affinity than predicted by curren

96 ), higher relative Slim (>30%) and decreased Ci under the ambient CO2 concentration (aCO2 ), with lea

97 formation energies of the C related defects Ci(SiI), CiOi, CiCs, and CiOi(SiI) with respect to the F

98 Ci processing probably by dephosphorylating Ci.

99 ration and interconversion between different Ci species catalyzed by carbonic anhydrases (CAs) are ke

100 ar concentration of Ci as measured by direct Ci uptake.

101 amily protein Costal 2 (Cos2), which directs Ci processing in Drosophila.

102 a putative vertebrate ortholog of Drosophila Ci, cause nephronophthisis type 7 in humans and mice.

103 ibed bigene cluster with a tandem duplicate, Ci-Dll-A.

104 Thus, cis-regulation of early Ci-Dll-B expression is activated by a required submodule

105 at Cos2 must bind to Fu to support efficient Ci-155 processing.

106 st protocol for the expression of eukaryotic Ci-VSD in Escherichia coli at milligram levels.

107 onse of photosynthesis to light and external Ci and their modulation of internal ribulose-1,5-bisphos

108 ned (Smo) and Zn-finger transcription factor Ci/Gli are crucial components in Hedgehog (Hh) signal tr

109 hat of vertebrates but only one GATA factor, Ci-GATAa, is expressed in the heart progenitor cells and

110 is dependent upon the transcription factors Ci-ets1/2 and Ci-mesp to generate cardiac progenitors.

111 binds to three regions of Gli1, just as for Ci, and that Cos2 functions to silence mammalian Gli1 in

112 hat this transport activity is essential for Ci-Slc26aalpha's in vivo function.

113 ve in the main transcriptional repressor for Ci acquisition genes, the NAD(P)H dehydrogenase transcri

114 for both Ciona Brachyury (Ci-Bra) and FoxA (Ci-FoxA-a).

115 The calculated mass fraction (Ci,aer/COA) of the individual SOA compounds showed eithe

116 ion causes PKA to switch its substrates from Ci to Smo within the Hh signalling complex (HSC).

117 The voltage dependence of the VSD from Ci-VSP (Ci-VSD) is dramatically right shifted, so that a

118 Like mammalian Lhx3 genes, Ci-Lhx3 encodes two isoforms with distinct N-terminal pe

119 d and essential for proper regulation of Gli/Ci proteins in the Hh pathway.

120 Here, we report that Dzip1 regulates Gli/Ci turnover by preventing degradation of speckle-type PO

121 While conserved in all members of the GLI/Ci family, the first two fingers do not appear to make s

122 l transduction pathways terminating with Gli/Ci transcription factors.

123 ownstream transcription factor NvGli (a Gli3/Ci ortholog) indicate that these genes may have conserve

124 )) resulted in dramatic decreases in growth, Ci uptake, and photosynthetic Ci affinity, especially at

125 tes are specifically bound in vitro by a GST-Ci-Bra fusion protein, and mutations that abolish bindin

126 In the presence of Hh, Ci-155 processing is blocked and Cos2 now promotes activ

127 In the absence of Hh, Ci/Gli processing is initiated by direct Pka phosphoryla

128 This newly identified Ci-Bra shadow enhancer contains binding sites with very

129 se to Hh, which collaborates with changes in Ci-specific activity to elicit a morphogenetic response.

130 Loss of emc leads to an increase in Ci(155) levels, nuclear migration, apical cell constrict

131 e citation rate for each tenfold increase in Ci.

132 ich confirms that HLA3 is indeed involved in Ci uptake, and suggests it is mainly associated with HCO

133 esting a unique voltage sensing mechanism in Ci-VSP.

134 We also explore the phosphorylated motifs in Ci that are recognized by Slimb and provide some evidenc

135 cs and voltage dependence of VSD movement in Ci-VSP can be tuned over 2 orders of magnitude and shift

136 Although the VSD operation in Ci-VSP exhibits original voltage dependence and kinetics

137 indicating the importance of this residue in Ci-VSP activation.

138 lpha-to-310 helical conversions of the S4 in Ci-VSP associated with voltage activation.

139 HLA3 is not expressed, resulted in increased Ci accumulation and Ci-dependent photosynthetic O2 evolu

140 f Slim by eCO2 was facilitated by increasing Ci , thus yielding a larger photosynthetic enhancement d

141 mily transcription factors and by increasing Ci/Gli-specific activity.

142 e involvement of additional NdhR-independent Ci-regulatory mechanisms.

143 -Cos2-kinase complexes that normally inhibit Ci activity and promote its processing.

144 Conversely, inhibiting Ci-MRF activity with antisense morpholinos down-regulate

145 tes Hh signaling activity through inhibiting Ci ubiquitination and degradation mediated by both Slimb

146 lasm through binding to SIN while inhibiting Ci/Gli activity in the nucleus depending on SIC.

147 s mechanistic insight into how Sufu inhibits Ci/Gli activity in the nucleus.

148 e molecular mechanism by which Sufu inhibits Ci/Gli activity remains poorly understood.

149 nic anhydrase CAH6 of the very high internal Ci caused by the defect in CAH3 provides Rubisco suffici

150 er transcription factor Cubitus interruptus (Ci(155)), the main effector of Hh signaling.

151 he transcription factor Cubitus interruptus (Ci) and G protein coupled receptor (GPCR) family protein

152 ion factors, Drosophila Cubitus interruptus (Ci) and mammalian Gli proteins.

153 activated Fu regulates Cubitus interruptus (Ci) by both promoting its transcriptional activator acti

154 The Gli/Cubitus interruptus (Ci) family of transcription factors acts at the downstre

155 ulating the activity of Cubitus interruptus (Ci) through phosphorylation of the Zn finger DNA-binding

156 g the repressor form of Cubitus interruptus (Ci), a Hh pathway antagonist, also results in expansion

157 matin binding sites for Cubitus interruptus (Ci), the transcription factor that mediates outputs of H

158 Drosophila Gli homolog, Cubitus interruptus (Ci), undergoes partial proteolysis to Ci-75, which repre

159 In Drosophila, Cubitus interruptus (Ci), which mediates hedgehog signaling, regulates gene e

160 tability of full-length Cubitus interruptus (Ci).

161 nt transcription factor cubitus interruptus (Ci).

162 he transcription factor Cubitus interruptus (Ci).

163 he transcription factor Cubitus interruptus (Ci).

164 e transcription factor, Cubitus interruptus (Ci).

165 tional mediator, called Cubitus interruptus (Ci).

166 ay transcription factor cubitus interruptus (Ci)/Gli by Cul3-Hedghog-induced MATH and BTB domain-cont

167 ls of homology with the Cubitus interruptus (Ci)/Gli family of proteins.

168 g pathway by inhibiting Cubitus interruptus (Ci)/Glioma-associated oncogene homolog (Gli) transcripti

169 the prototypic VSP from Ciona intestinalis, Ci-VSP, we generated chimeric proteins that are voltage-

170 te of a significantly elevated intracellular Ci concentration.

171 We studied intracellular Ci limitation in the slow-growing CO2/HCO3 (-)-uptake mu

172 Synechocystis sp. PCC 6803 to intracellular Ci limitation and may become a valuable reference for im

173 ance of photorespiration under intracellular Ci limitation.

174 /3 targets, some of which overlap with known Ci-Bra-downstream notochord genes.

175 Levels of latent Ci are controlled by degradation, with different pathway

176 CO2 levels in leaves (Ci) are determined by respiration, photosynthesis, stoma

177 sing requires phosphorylation of full-length Ci (Ci-155) by protein kinase A (PKA), casein kinase 1 (

178 2 phosphorylation and activating full-length Ci by antagonizing Su(fu) and by other mechanisms.

179 omplex regulates the cleavage of full-length Ci to a truncated repressor protein, Ci75, in a process

180 a full Hh response, stabilizing full-length Ci via Cos2 phosphorylation and activating full-length C

181 necessary for full activation of full-length Ci-155 and Gli transcription factors in response to Hh p

182 l and thus signaling activity of full-length Ci.

183 edgehog (Hh) signaling activates full-length Ci/Gli family transcription factors and prevents Ci/Gli

184 ockdown of LCIA mRNA (which encodes limiting-Ci-inducible plastid envelope protein reported to transp

185 th mutations in LCIB (which encodes limiting-Ci-inducible plastid-localized protein required for norm

186 ca1ca4 plants suggest that more than a low [Ci ]-dependent pathway may function in red light-induced

187 reacclimates cyanobacterial cells to lowered Ci supply under HC conditions.

188 ced relaxation EPR spectroscopy measurement, Ci-VSD reconstitutes essentially randomly in proteolipos

189 , S572 and S931, which is thought to mediate Ci-155 activation, is not required for normal activation

190 erminal regions of Ci, both of which mediate Ci degradation.

191 Each gel was radiolabeled with 500 micro Ci of (99m)Technetium-diethylene triaminepentaacetic aci

192 simple in vivo assay based on misexpressing Ci-MRF in the notochord of Ciona embryos.

193 urrent models, being saturated by 5 to 15 mm Ci.

194 d that a specific Ephrin signaling molecule, Ci-ephrin-Ad, is required to establish asymmetric MAPK s

195 Mice treated with anti-CD20 PRIT and 600 mu Ci [(2)(1)(3)Bi]DOTA-biotin exhibited marked tumor growt

196 2/3 notochord CRM that necessitates multiple Ci-Bra binding sites for its activity.

197 ain-containing proteins, which we have named Ci-Gla1 through Ci-Gla4.

198 affold yielded similar results as the native Ci-VSP S4.

199 lastid-localized protein required for normal Ci uptake or accumulation in low-CO(2) conditions) and/o

200 he Ala-Thr dipeptide is necessary for normal Ci-MRF function, and that while eliminating the C/H doma

201 Here, we show that normal Ci-155 activation by Hh requires Cos2 binding to Fu, sup

202 gh microarray screens, we uncovered numerous Ci-Tbx2/3 targets, some of which overlap with known Ci-B

203 on, is not required for normal activation of Ci-155 by Hh or by activated Fu.

204 blocked and Cos2 now promotes activation of Ci-155, which requires Fu kinase activity.

205 n by regulating the levels and activities of Ci/Gli family transcription factors.

206 tion, regulating the amounts and activity of Ci that ultimately interpret the level of Hh to which ce

207 tivity and revealed the myogenic activity of Ci-MRF by inducing the expression of four muscle marker

208 quisite for generating sufficient amounts of Ci-VSD protein for high-resolution structural studies.

209 crease in the intracellular concentration of Ci as measured by direct Ci uptake.

210 We demonstrate that experimental control of Ci-VSPTEN can be obtained either by electrophysiological

211 ocopy is associated with the deregulation of Ci control, an overreduced cellular state, and limited p

212 ence that Cos2 binding to the CORD domain of Ci-155 contributes to both Ci-155 processing and Ci-155

213 ttern mimicking the endogenous expression of Ci-Dll-B at gastrula stages.

214 tor induction through targeted expression of Ci-Integrin beta2.

215 sphorylation of Ci leads to the formation of Ci repressor form.

216 lations by interfering with the formation of Ci-Sufu and Ci-Cos2-kinase complexes that normally inhib

217 Expression of mutant forms of Ci-Tbx2/3 in the developing notochord revealed a role fo

218 es both the activator and repressor forms of Ci.

219 mids aimed to interfere with the function of Ci-leprecan and categorized the resulting phenotypes, wh

220 cription factor Gli2, a mammalian homolog of Ci, results in severe skeletal abnormalities in mice.

221 d SIN are required for optimal inhibition of Ci/Gli by Sufu.

222 Injection of Ci-MRF mRNA into eggs resulted in increased embryonic mu

223 Our data suggest multiple layers of Ci regulation, including inversely regulated modules of

224 homolog of Spop, and increased the level of Ci.

225 the HSC, and in which the relative levels of Ci and Smo within the HSC determine differential activat

226 alpha and CK2beta increases the half-life of Ci.

227 Conversely, misexpression of Ci-ephrin-Ad in the endoderm induces ectopic activation

228 Here, we show that phosphorylation of Ci at the specific PKA, GSK-3, and CK1 sites required in

229 on, whereas PKA-dependent phosphorylation of Ci leads to the formation of Ci repressor form.

230 When Hh is absent, phosphorylation of Ci/Gli triggers binding to SCF ubiquitin ligase complexe

231 ntly affect activator and repressor pools of Ci.

232 by inhibiting the proteolytic processing of Ci/Gli family transcription factors and by increasing Ci

233 ubiquitin/proteasome-mediated proteolysis of Ci/Gli transcription factors is central to Hh signaling,

234 ding of Sufu to SIC and the middle region of Ci can impede recruitment of the transcriptional coactiv

235 binding primarily through a nearby region of Ci, which might contact an SCF component other than Slim

236 its binding site in the C-terminal region of Ci.

237 fu-binding motif in the C-terminal region of Ci/Gli and provides mechanistic insight into how Sufu in

238 HIB binds the N- and C-terminal regions of Ci, both of which mediate Ci degradation.

239 roles played by Su(fu) in the regulation of Ci.

240 and provide some evidence that silencing of Ci-155 by phosphorylation may involve more than binding

241 Studies of Ci variants with altered CK1 and GSK3 sites suggest that

242 B cluster reveals a 378bp region upstream of Ci-Dll-B, termed B1, which is highly conserved with the

243 tal limitation of CO2 uptake, by control of [Ci ], was eliminated.

244 I individually has no demonstrable effect on Ci-MRF, simultaneous loss of both motifs significantly r

245 e evidence for an analogous action of PKA on Ci.

246 ple successively phosphorylated CK1 sites on Ci create an atypical extended binding site for the SCF

247 ntee optimal growth under excessive light or Ci limitation.

248 ses the level of Smo, which then outcompetes Ci for association with PKA and causes a switch in PKA s

249 The voltage-sensitive phosphatase Ci-VSP consists of an intracellular phosphatase domain (

250 The voltage-dependent phosphatase (Ci-VSP), which does not have a conducting pore, shows th

251 intestinalis voltage-sensitive phosphatase (Ci-VSP) does not exhibit extended and long-lived 310 con

252 intestinalis voltage-sensitive phosphatase (Ci-VSP) represents the first discovered member of enzyme

253 ses in growth, Ci uptake, and photosynthetic Ci affinity, especially at pH 9, at which HCO(3)(-) is t

254 g the effect of pH on growth, photosynthetic Ci affinity, and [(14)C]-Ci uptake in very low CO(2) con

255 igh-pH-dependent decreases in photosynthetic Ci affinity and Ci uptake, the combination of nearly com

256 find that Wdb counteracts kinases to prevent Ci phosphorylation.

257 li family transcription factors and prevents Ci/Gli proteolytic processing to repressor forms.

258 We showed that CK2 prevents Ci ubiquitination and degradation by the proteasome.

259 CK2beta RNAi promotes Ci degradation whereas coexpressing CK2alpha and CK2beta

260 The ascidian VSD protein Ci-VSP gates a phosphatase activity rather than a channe

261 Reducing Ci-ephrin-Ad activity via morpholino injection results i

262 r divergence in the mechanisms that regulate Ci/Gli protein activities, including the role of the kin

263 are attenuated in their ability to regulate Ci activity and exhibit phenotypes consistent with atten

264 H) protein Extramacrochaetae (Emc) regulates Ci(155) levels.

265 mine the mechanism by which Su(fu) regulates Ci import by investigating the importance of the Ci nucl

266 osphatase Puc, the transcriptional regulator Ci, and the chromatin component MacroH2A.

267 ing site to an "exposed" location can render Ci resistant to Sufu-mediated inhibition in the nucleus.

268 ression of Ciona Tbx2/3 (Ci-Tbx2/3) requires Ci-Bra, and identified a Ci-Tbx2/3 notochord CRM that ne

269 Residual Ci-155 processing in the absence of Cos2-Fu interaction

270 smembrane Smoothened (Smo) proteins reverses Ci/Gli inhibition by Suppressor of Fused (SuFu) and kine

271 We show that Sufu can sequester Ci/Gli in the cytoplasm through binding to SIN while inh

272 indicating a deficiency in a L-CO2-specific Ci uptake and accumulation system.

273 engineered voltage-sensitive enzymes, termed Ci-VSPTEN, is the novel ability to switch enzymatic acti

274 The Ci(SiI)(2+) state dominates in almost the whole Fermi en

275 The Ci-Dll-B gene is an early regulator of ectodermal develo

276 The Ci/Gli family of transcription factors mediates Hedgehog

277 Among the Ci-Tbx2/3 notochord targets are evolutionarily conserved

278 We examined the Ci-dependent transcriptional and metabolic regulation in

279 icrocystis displays genetic variation in the Ci uptake systems BicA and SbtA, where BicA has a low af

280 prolonged depolarization was studied in the Ci-VSP by using electrophysiological and site-directed f

281 x-ray structures, its transplantation in the Ci-VSP VSD scaffold yielded similar results as the nativ

282 nt that generates the repressor forms of the Ci and Gli transcription factors that keep target genes

283 mport by investigating the importance of the Ci nuclear localization signal (NLS) and the effect of a

284 By screening approximately 14 kb of the Ci-leprecan locus for cis-regulatory activity, we have i

285 TRA-1, a member of the Ci/Gli family of transcriptional repressors, plays an es

286 trained model of the hHv1 dimer based on the Ci-VSD structure at resting state.

287 roteins, which we have named Ci-Gla1 through Ci-Gla4.

288 Thus, Ci-VSPTEN provides a novel approach for studying the com

289 serpentine protein Smoothened (Smo) leads to Ci activation, whereas PKA-dependent phosphorylation of

290 uptus (Ci), undergoes partial proteolysis to Ci-75, which represses key Hh target genes.

291 ing of a ubiquitin-specific protease Usp7 to Ci, which positively regulates Hh signaling activity thr

292 om the preacclimation of the transcriptional Ci regulator mutant ndhR (for ndhF3 operon transcription

293 The 155-bp sequence contains two Ci-Bra binding sites with identical core sequences but o

294 report, we characterize the function of two Ci domains that are conserved in the vertebrate homologu

295 e voltage dependence of the VSD from Ci-VSP (Ci-VSD) is dramatically right shifted, so that at 0 mV i

296 lake, bicA + sbtA strains were dominant when Ci concentrations were depleted during a dense cyanobact

297 rains with only the high-flux bicA gene when Ci concentrations increased later in the season.

298 We also show that when Ci proteolysis is compromised, its specific activity is

299 ndings reveal a conserved mechanism by which Ci/Gli is stabilized by a deubiquitination enzyme and id

300 iofuel production in cyanobacteria, in which Ci is channeled off from central metabolism and may thus

301 terns' of transcription factors partner with Ci to make Hh-dependent gene expression position specifi