ライフサイエンスコーパス: Ciona

1 o form the atrium and atrial siphon of adult Ciona.

2 nomic organisation, albeit more divergent in Ciona.

3 participate in notochord differentiation in Ciona.

4 cis-regulatory sequences between Molgula and Ciona.

5 llow for rapid screening of gene function in Ciona.

6 xplain and reproduce the neuronal pattern in Ciona.

7 iple classes of MRF-regulated genes exist in Ciona.

8 egies in the elucidation of gene function in Ciona.

9 encoded at four loci (i.e., VCBPA-VCBPD) in Ciona, a urochordate, and are expressed by distinct epit

10 However, predators reduced Ciona abundance much more than that of Ascidia at severa

11 d recruit stages had modest to no effects on Ciona abundance.

12 ppears to be the only embryonic structure in Ciona activating the PCP pathway.

13 The closest homologs to RPE65 in Ciona and Branchiostoma lacked predicted functionally di

14 ear to be the major target of methylation in Ciona and honey bee.

15 any genes expressed in B4.1 lineages in both Ciona and the distantly related ascidian Halocynthia.

16 Interestingly, the alignment of Ciona and vertebrate TERT sequences reveals a previously

17 All ABC protein subfamilies found in Ciona and zebrafish correspond to the human subfamilies,

18 gest that a midbrain homologue is missing in Ciona, and argue that midbrain development is a novelty

19 enomic comparisons restricted to Drosophila, Ciona, and humans (protostomes, urochordates, and verteb

20 Furthermore, Ciona appeared to be a weaker competitor at the adult st

21 Under some circumstances, Ciona became abundant in mid-successional stages and sho

22 l number of binding motifs are necessary for Ciona betagamma-crystallin expression, and narrow down t

23 The Ciona betagamma-crystallin is only expressed in the palp

24 The crystal structure of Ciona betagamma-crystallin is very similar to that of a

25 The Ciona betagamma-crystallin promoter region targeted expr

26 M contains functional binding sites for both Ciona Brachyury (Ci-Bra) and FoxA (Ci-FoxA-a).

27 We found that Ciona Brachyury (Ci-Bra) controls most of its targets di

28 ens were recently used to identify potential Ciona Brachyury (Ci-Bra) target genes.

29 the notochord-specific transcription factor Ciona Brachyury (Ci-Bra).

30 The Ciona Brachyury gene (Ci-Bra) is regulated, in part, by

31 bp enhancer from the promoter region of the Ciona Brachyury gene (Ci-Bra), which is sufficient to di

32 er on the regulation of a notochord-specific Ciona Brachyury gene (Ci-Bra).

33 The notochord of the ascidian Ciona consists of only 40 cells, and is a longstanding m

34 ndicate that notochord cell intercalation in Ciona does not require the progressive signals which coo

35 Our results identify two domains of Ciona ectoderm that are marked by the genetic cascade th

36 oration of lacZ fusion genes into fertilized Ciona eggs.

37 Finally, this study establishes the Ciona embryo as an effective in vivo system for the stud

38 ell lineages and molecular strategies in the Ciona embryo.

39 homeobox gene bix in notochord or muscle of Ciona embryos as a means of interfering with development

40 Ciona embryos expressing bix in the notochord from the 6

41 h mediates expression in the neural plate of Ciona embryos in response to fibroblast growth factor (F

42 for specification of the cardiac mesoderm in Ciona embryos.

43 ion of ZicL and ETS transcription factors in Ciona embryos.

44 ized patterns of expression in tadpole-stage Ciona embryos.

45 ficient incorporation of transgenic DNA into Ciona embryos.

46 er/Olf/EBF) gene in hundreds of synchronized Ciona embryos.

47 on misexpressing Ci-MRF in the notochord of Ciona embryos.

48 yogenesis in the tail of the simple chordate Ciona exhibits a similar reliance on its single MRF-fami

49 A dominant-negative form of the Ciona FGF receptor suppresses the formation of polarized

50 Inhibition of Ciona FGF3 activity results in similar defects, even tho

51 , techniques, and resources available to the Ciona geneticist, citing examples of studies that employ

52 The recent sequencing of a second Ciona genome (C. savignyi) permits the use of simple ali

53 The Ciona genome contains approximately 16,000 protein-codin

54 sequencing of staged EST libraries make the Ciona genome one of the best annotated among those that

55 as clusters of identical sites found in the Ciona genome with different arrangements are unable to a

56 spects of creating a regulatory atlas of the Ciona genome, whereby every enhancer is identified for e

57 ttlement and colonization of bacteria in the Ciona gut.

58 124 silences Notch, Neuralized and all three Ciona Hairy/Enhancer-of-Split genes.

59 ed the isolation of a heart enhancer for the Ciona Hand-like gene.

60 These observations provide evidence that Ciona has a neurogenic proto-placode, which forms neuron

61 Ciona has a small genome, and simple, well-defined embyr

62 nificantly advances our understanding of the Ciona heart gene network.

63 The Ciona heart progenitor lineage is first specified by Fib

64 on analyses was used to demonstrate that the Ciona HNF-3beta homologue is expressed in the ventralmos

65 Here we present evidence that a Ciona homolog of snail (Ci-sna) encodes a repressor of t

66 We used the ascidian Ciona, in which the single-copy Brachyury is notochord-s

67 Caenorhabditis elegans (Ce), the sea squirt Ciona intestinalis (Ci) and amphioxus Branchiostoma flor

68 single MRF gene of the invertebrate chordate Ciona intestinalis (Ci-MRF).

69 s was recently identified in the urochordate Ciona intestinalis (CiCBR).

70 euterostomian invertebrate - the urochordate Ciona intestinalis - that is orthologous to vertebrate c

71 eart progenitor cells of the simple chordate Ciona intestinalis also generate precursors of the atria

72 rization of TERT genes from two sea squirts, Ciona intestinalis and Ciona savignyi.

73 D family gene was identified in the ascidian Ciona intestinalis and designated CiMDF (Ciona intestina

74 lated organisms that make cellulose, such as Ciona intestinalis and Dictyostelium discoideum, reveale

75 sms, Drosophila melanogaster, Daphnia pulex, Ciona intestinalis and Strongylocentrotus purpuratus.

76 macaque and Opossum, the chordate genome of Ciona intestinalis and the import and integration of the

77 t absent from the genomes of the urochordate Ciona intestinalis and the lower eukaryotes D. melanogas

78 using a simple method to introduce DNA into Ciona intestinalis and the several available tissue-spec

79 We identified putative MBL homologs in Ciona intestinalis and Trichoplax adhaerens, and investi

80 g the notochord of the invertebrate chordate Ciona intestinalis as a model.

81 Using Ciona intestinalis as a simple chordate model, we show t

82 ique phylogenetic position of the sea squirt Ciona intestinalis as part of the sister group to the ve

83 nderlying basis of enhancer activity for the Ciona intestinalis betagamma-crystallin gene, which driv

84 A chordate ortholog of UNC-3, Ciona intestinalis COE, was also both required and suffi

85 Here we show that the gut mucosa of Ciona intestinalis contains an extensive matrix of chiti

86 sed to improve and enrich the description of Ciona intestinalis embryonic development, based on an im

87 Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for char

88 revised cell lineage of the pigment cells in Ciona intestinalis embryos.

89 Here we show that the tunicate Ciona intestinalis exhibits a proto-placodal ectoderm (P

90 POUIV gene families to examine the tunicate Ciona intestinalis for evidence of structures homologous

91 s, making for a direct orthology between the Ciona intestinalis gene CiPax6 and Pax6 in mammals.

92 d the distribution of DNA methylation in the Ciona intestinalis genome.

93 we estimated that the invertebrate chordate Ciona intestinalis has 15,500 protein-coding genes (+/-3

94 The ascidian Ciona intestinalis has a monolobal transferrin (nicatran

95 A forward genetic screen in the ascidian Ciona intestinalis identified a mutant line (frimousse)

96 The invertebrate chordate Ciona intestinalis is a widely used model organism in bi

97 t specification in the invertebrate chordate Ciona intestinalis is similar to that of vertebrates but

98 ian Ciona intestinalis and designated CiMDF (Ciona intestinalis Muscle Determination Factor).

99 y through mediation of Ca(2+) transients, in Ciona intestinalis neural induction.

100 Here we show that the tunicate Ciona intestinalis possesses a cephalic melanocyte linea

101 estigate this process in the simple chordate Ciona intestinalis Previous studies have implicated Noda

102 s trunk ventral cells, TVCs) of the ascidian Ciona intestinalis provide a simple chordate model with

103 the CNS of the tadpole larva of the ascidian Ciona intestinalis provides us with a chordate nervous s

104 ans and even a highly divergent invertebrate Ciona intestinalis qualitatively and quantitatively supp

105 motivated by our experiments in the ascidian Ciona intestinalis showing that the peripheral sensory n

106 stinct moto- and interneuron subtypes in the Ciona intestinalis tadpole larva.

107 ntrols the rhythmic swimming behavior of the Ciona intestinalis tadpole.

108 ed several cDNAs derived from the sea squirt Ciona intestinalis that encode vitamin K-dependent prote

109 -induced short-tailed mutant in the ascidian Ciona intestinalis that is the product of a premature st

110 identified in the primitive ascidian species Ciona intestinalis that possesses the characteristic fea

111 Here, we employ the invertebrate chordate Ciona intestinalis to delineate an essential in vivo rol

112 rphological simplicity of the basal chordate Ciona intestinalis to elucidate Mesp regulation and func

113 loit wild populations of the marine chordate Ciona intestinalis to show that levels of buffering are

114 invasive tunicates, Ciona robusta (formerly Ciona intestinalis type A) and C. intestinalis (formerly

115 inalis type A) and C. intestinalis (formerly Ciona intestinalis type B), globally distributed and sym

116 results suggest that the native S4 from the Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP

117 The Ciona intestinalis voltage-sensitive phosphatase (Ci-VSP

118 ht consists of the voltage-sensing domain of Ciona intestinalis voltage-sensitive phosphatase and sup

119 2 K(+) channel and the voltage sensor of the Ciona intestinalis voltage-sensitive phosphatase, agains

120 rents are significantly faster than those of Ciona intestinalis voltage-sensitive phosphatase.

121 The genome of the invertebrate chordate Ciona intestinalis was found to be a stable mosaic of me

122 ge-gated proton channels in humans, mice and Ciona intestinalis were discovered.

123 ogs transcribed in eggs of Xenopus laevis or Ciona intestinalis were found, pinpointing evolutionary

124 Eggs of the ascidian Ciona intestinalis were injected with the Src-homology 2

125 ses fewer substitutions than the sea squirt (Ciona intestinalis) CB1 sequence.

126 uccess of an introduced marine invertebrate (Ciona intestinalis) in Northern California.

127 identified ABC proteins from the sea squirt (Ciona intestinalis), zebrafish (Danio rerio), and chicke

128 -3beta and snail homologues in the ascidian, Ciona intestinalis, a member of the subphylum Urochordat

129 We have examined ASICs from the ascidian Ciona intestinalis, a simple chordate organism whose ner

130 ting the ARNTL gene family in the genomes of Ciona intestinalis, amphioxus, zebrafish, and human.

131 the zebrafish (Danio rerio) and the ascidian Ciona intestinalis, an invertebrate chordate belonging t

132 enic regulatory factor (MRF) of the ascidian Ciona intestinalis, an invertebrate chordate.

133 In ascidians, such as Ciona intestinalis, Brachyury is expressed exclusively i

134 subsequently identified in human, mouse, and Ciona intestinalis, but their existence in dinoflagellat

135 mo sapiens, Mus musculus, Takifugu rubripes, Ciona intestinalis, Caenorhabditis elegans, Drosophila m

136 he voltage sensor of the prototypic VSP from Ciona intestinalis, Ci-VSP, we generated chimeric protei

137 The ascidian Ciona intestinalis, commonly known as a 'sea squirt', ha

138 the genome of a urochordate, the sea squirt, Ciona intestinalis, did not turn up any genuine ortholog

139 nt evidence that the embryo of the ascidian, Ciona intestinalis, is an easily manipulated system for

140 In Ciona intestinalis, leprecan was identified as a target

141 e demonstrate that, in the chordate ascidian Ciona intestinalis, miR-124 plays an extensive role in p

142 of the gene for the FlgCK from the tunicate Ciona intestinalis, providing support for the linkage of

143 rithm on simulated next-generation data from Ciona intestinalis, real next-generation data from Droso

144 In the ascidian, Ciona intestinalis, snail (Ci-sna) is expressed at the 3

145 ll type in the tadpole larva of the tunicate Ciona intestinalis, the bipolar tail neuron, shares a se

146 In the simple chordate Ciona intestinalis, the notochord plate consists of just

147 In Ciona intestinalis, the palps consist of three conical p

148 tic manipulability, we chose the sea squirt, Ciona intestinalis, to explore intraspecies sequence com

149 tion and streamlined genome of the ascidian, Ciona intestinalis, to investigate heart development in

150 on pattern of CiMDF, the MyoD-family gene of Ciona intestinalis, was analyzed in unmanipulated and mi

151 The draft genome of the primitive chordate, Ciona intestinalis, was published three years ago.

152 anogaster and in the non-vertebrate chordate Ciona intestinalis, which each have only one talin gene,

153 We show that the urochordate Ciona intestinalis, which split from the vertebrate line

154 e larva of a sibling chordate, the ascidian, Ciona intestinalis.

155 ervous system of the ascidian tadpole larva, Ciona intestinalis.

156 -bp notochord-specific CRM from the ascidian Ciona intestinalis.

157 expression patterns of NCLC in the ascidian Ciona intestinalis.

158 alyze cardiac cell migration in the ascidian Ciona intestinalis.

159 C. savignyi to its divergent sister species, Ciona intestinalis.

160 es was performed by using the basal chordate Ciona intestinalis.

161 fied in the genome sequences of the ascidian Ciona intestinalis.

162 regions in the ascidians Ciona savignyi and Ciona intestinalis.

163 or tissue-specific enhancers in the ascidian Ciona intestinalis.

164 al tube closure in the invertebrate chordate Ciona intestinalis.

165 of the genome of the most studied ascidian, Ciona intestinalis.

166 mbe, Drosophila melanogaster, zebrafish, and Ciona intestinalis.

167 gene, CiVegTR, was isolated in the ascidian Ciona intestinalis.

168 ng dynamics using the invertebrate chordate, Ciona intestinalis.

169 mechanism for zippering in a basal chordate, Ciona intestinalis.

170 n the CNS of a simple chordate, the ascidian Ciona intestinalis.

171 anded breaks in the genome of the sea squirt Ciona intestinalis.

172 PNS development of the invertebrate chordate Ciona intestinalis.

173 ence to investigate cilia in the urochordate Ciona intestinalis.

174 latory network in the invertebrate chordate, Ciona intestinalis.

175 or of ectodermal development in the ascidian Ciona intestinalis.

176 e lens of transgenic vertebrates despite the Ciona lineage predating the evolution of the lens.

177 In Ciona, matrix adhesion polarizes fibroblast growth facto

178 In some chordates, including the ascidian Ciona, members of the Tbx2 subfamily of T-box genes are

179 zation of a minimal cardiac enhancer for the Ciona Mesp gene demonstrated direct activation by the T-

180 gulatory elements that drive coexpression of Ciona muscle genes and obtained quantitative estimates o

181 We discovered that in Ciona, MyT1, Pou4, Atonal, and NeuroD-like are expressed

182 Here we show that the Ciona neurohypophyseal canal is present from the end of

183 oteins or particular factors present only in Ciona neurons.

184 stablishment of asymmetric cell fates in the Ciona notochord and neural tube.

185 Moreover, the Ciona notochord as a single-file array of forty polarize

186 The Ciona notochord displays planar cell polarity (PCP), wit

187 Orthologs of 112 of the Ciona notochord genes have known notochord expression in

188 The full set of Ciona notochord genes provides a foundation for systems-

189 om multiple stages to define a comprehensive Ciona notochord transcriptome.

190 changed and then restored, underscoring the Ciona notochord's amenability for in vivo studies of PCP

191 Dishevelled serves a similar function in the Ciona notochord.

192 Much research has focused on Ciona or Halocynthia spp. but development in other ascid

193 RPE65 nor LRAT orthologs occur in tunicates (Ciona) or cephalochordates (Branchiostoma), but occur in

194 diac genes were analyzed, including the sole Ciona ortholog of the Drosophila tinman gene, and tissue

195 The Ciona orthologues of seven of sixteen genes that functio

196 The Ciona PPE is shown to produce ciliated neurons that expr

197 In Ciona pre-cardiac founder cells, invasion of the underly

198 The notochord of the ascidian Ciona provides a unique model for investigating the PCP

199 ession of Dmbx genes from amphioxus and from Ciona, representing the two most closely related lineage

200 ibility that the PPE-derived GnRH neurons of Ciona resemble an ancestral cell type, a progenitor to t

201 tions of these proteins in Branchiostoma and Ciona, respectively, providing an insight into the ances

202 h MEK or Fgfr inhibitor at tailbud stages in Ciona results in a larva which fails to form atrial plac

203 Comparisons to the homologous lineage in Ciona revealed identical cell division and fate specific

204 is is exemplified by two invasive tunicates, Ciona robusta (formerly Ciona intestinalis type A) and C

205 sion during oral siphon (OS) regeneration in Ciona robusta, and the derived network of their interact

206 ural selection in a model invasive ascidian, Ciona robusta.

207 nificant progress has been made in utilizing Ciona's genomic and morphological simplicity to better u

208 lyses of regulatory regions in the ascidians Ciona savignyi and Ciona intestinalis.

209 The urochordate Ciona savignyi is an emerging model organism for the stu

210 Here we show that the recessive short-tailed Ciona savignyi mutation chongmague (chm) has a novel def

211 affecting early development in the ascidian Ciona savignyi resulted in the isolation of a number of

212 l mutation in the gene dmrt1 in the ascidian Ciona savignyi results in profound abnormalities in the

213 a cellulose synthase gene from the ascidian Ciona savignyi that is expressed in the epidermis.

214 nd example of the genomes of the sea squirt (Ciona savignyi) and the mosquito (Aedes aegypti).

215 We show that the urochordate Ciona savignyi, one of the species of ocean-dwelling bro

216 ped to assemble the genome of the sea squirt Ciona savignyi, which was sequenced to a depth of 12.7 x

217 from two sea squirts, Ciona intestinalis and Ciona savignyi.

218 elements of 20 muscle genes in the chordate, Ciona savignyi.

219 simple organ: the notochord of the ascidian Ciona savignyi.

220 h the corresponding region from the congener Ciona savignyi.

221 pendymal cells of the neural tube, while the Ciona snail homologue is expressed at the junction betwe

222 omparative sequence analysis between the two Ciona species for guiding gene regulatory experiments.

223 ensive Notch pathway targeting appears to be Ciona specific.

224 We show that Ciona ss-carotene monooxygenase a (BCMOa) (previously an

225 es are typically found in simplified form in Ciona, suggesting that ascidians contain the basic ances

226 turbation of the FGF pathway in the ascidian Ciona support a similar role for this pathway: inhibitio

227 Here we present evidence that a Ciona Suppressor of Hairless inverted question markCi-Su

228 The formation of the Ciona tadpole depends on simple, well-defined cellular l

229 The Ciona tadpole is constructed from simple, well-defined c

230 Ciona tadpole larvae exhibit a basic chordate body plan

231 as the sensory vesicle (simple brain) of the Ciona tadpole.

232 determined that the notochord expression of Ciona Tbx2/3 (Ci-Tbx2/3) requires Ci-Bra, and identified

233 The Ciona TERT gene is expressed in all tissues analyzed exc

234 Both Ciona TERTs contain all of the reverse transcriptase (RT

235 In Ciona, the CiDmbx gene is detected in neural cells cauda

236 In ascidians such as Ciona there is a particularly intimate relationship betw

237 tochord intercalation are cell-autonomous in Ciona, though such defects have nonautonomous effects in

238 ident community in limiting the abundance of Ciona through experiments conducted on fertilization, la

239 a new member of the tropomyosin superfamily, Ciona tropomyosin (Ci-trop).

240 tion between CiMRF and an essential E-box of Ciona Troponin I is required for the expression of this

241 cardiopharyngeal mesoderm in the urochordate Ciona, where a related gene regulatory network determine

242 e from embryonic development in the ascidian Ciona, which is dependent upon the transcription factors