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1 nctional, N-hydroxysuccinimidyl fluorophore (Cy3).
2 tions of the NTA derivative, (Ni(2+)-NTA)(2)-Cy3.
3 the signal of the apoptosis marker Annexin V-Cy3.
4 cDNA of the healthy co-twin was labeled with cy3.
5 pping a 15-basepair DNA segment labeled with Cy3.
6 asuring diffusional transfer of biocytin and Cy3.
7 erative visualization of tissue stained with Cy3.
8 proximately 110 positive genes detected with Cy3.
9 were detected by RLS compared to labeling by Cy3.
10 orochrome FITC and the acceptor fluorochrome Cy3.
11 odies conjugated to the orange emitting dye, Cy3.
12 d bis-styryl (MeOPh)2BODIPY dyes relative to Cy3.
13 to allow (111)In labeling or the fluorophore Cy3.
14 an extrinsic fluorophore [fluorescein (F) or Cy3].
20 anthocyanins in commercialised samples were Cy3,5dG (19.30 +/- 3.47 mg/L), followed by Dp3,5dG (17.8
23 ort that small molecules, such as cyanine 3 (Cy3), a synthetic fluorescent molecule, and 4-hydroxy-3-
24 ransfer (FRET) from the fluorescein donor to Cy3 acceptor by splitting these two dyes into separate f
30 -actin assembly after activation of Rac, but Cy3-actin is not incorporated into stress fibers formed
31 mbly, fluorescently labeled monomeric actin, Cy3-actin, was introduced into serum-starved Swiss 3T3 f
39 ("targets") were hybridized with colabeled (Cy3 and biotin) human lung cDNA probes at concentrations
40 etween DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to monitor the re
41 transfer efficiencies between the juxtaposed Cy3 and Cy5 5'-end labeled viral DNA ends in the synapti
42 n and emission wavelengths of the two labels Cy3 and Cy5 allow for fluorescence resonance energy tran
44 ructure of the indocarbocyanine fluorophores Cy3 and Cy5 attached to DNA via three-carbon atom tether
46 labeled, but are spectrally compatible with Cy3 and Cy5 dye-labeled targets when using confocal lase
49 NA derived from the same source was used for Cy3 and Cy5 labeling and hybridized to the same array, s
51 y crystallography to analyze the location of Cy3 and Cy5 on dsRNA, using complexes of an RNA stem-loo
54 ged with fluorescein and are compatible with Cy3 and Cy5 target labeling dyes when using confocal las
55 conjugated with the cyanine dye fluorophores Cy3 and Cy5 to quantify the melting/rebinding reaction b
57 However, the manner of interaction of both Cy3 and Cy5 with the terminus of the dsRNA is significan
59 of nanofibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes in
60 at the efficiency of energy transfer between Cy3 and Cy5, that are attached to nucleic acids in this
61 from differences in the detection limits of Cy3 and Cy5-labeled cDNA, a potential concern for array
66 istinct (approximately 250 nm) fluorophores, Cy3 and IRD800, were excited simultaneously using two di
68 hiocyanate (FITC) or to the carbocyanine dye Cy3 and used to label cytokine-responsive human hematopo
71 nor for energy transfer with both Cyanine 3 (Cy3) and Alexa Fluor 647 (A647) fluorescent dyes associa
72 ary donor array (Py), secondary donor array (Cy3) and an acceptor (AF) with defined interchromophore
73 eled on its 3'-end with a fluorescent donor (Cy3) and on its 5'-end with a fluorescent acceptor (Cy5)
74 g ovarian cancer showed that the model drug (Cy3) and polymer bound to Cy5 were colocalized at an ear
75 metric analysis of the emission from the QD, Cy3, and A647 permits discrimination between thrombin an
76 labeled with a fluorescence donor molecule, Cy3, and fluorescence acceptor molecule, Cy5, and by var
77 obleaching (FRAP) experiments confirmed that Cy3- and BODIPY-FL-labeled ferritin and PZn(2) exhibited
78 ction was demonstrated in this work by using Cy3- and Cy5-doped nanoparticles in sandwich hybridizati
82 ined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distanc
84 tudies indicate that this occurs because the Cy3 anti-GFAP selectively binds the intermediate filamen
85 vivo studies, retinal flatmounts exposed to Cy3 anti-GFAP showed minimal surface fluorescence, but e
88 intravenous injection of Lac-GLN containing Cy3-anti-miR-155 led to preferential accumulation of the
89 ein (emission maximum approximately 540 nm), Cy3 (approximately 570 nm), Cy5 (approximately 670 nm),
93 t labeled with fluorescein at the 5' end and Cy3 at the 3' end show that modifications required for l
96 nformational dynamics of the fluorescent dye Cy3 attached to the 5' end of double-stranded DNA using
97 etramethylindocarbocyanine-5,5'-disulfonate (Cy3) attached via either a short or long linker at the C
99 rent donor-acceptor dye pairs (TMR-ATTO647N, Cy3-ATTO647N, TMR-Cy5, and Cy3-Cy5), we find that this p
100 factors of 10-fold for Cy5 and 2.5-fold for Cy3, because of the smaller amount of immobilized fluoro
104 wo fluorophores used in aCGH-usually Cy5 and Cy3-can be observed as a bias within the intensity distr
105 majority of GFP-N-WASP undergoing FRET with Cy3-Cdc42 is localized within a transferrin receptor- an
106 vidually transfected TSHR(GFP) and TSHR(Myc):Cy3 cells cultured together and also by acceptor photobl
107 localized, contaminating fluorescence in the Cy3 channel on several commercial and in-house printed m
108 fluorescence microscopy to directly observe Cy3-Cof1 and Cy3-Cof2 interacting with actin filaments i
109 microscopy to directly observe Cy3-Cof1 and Cy3-Cof2 interacting with actin filaments in real time d
111 ular trafficking of AAV-2 using fluorescent (Cy3)-conjugated viral particles and molecular techniques
113 inals of hippocampal neurons prelabeled with CY3-conjugated antibodies directed against lumenal epito
114 (FRET) using fluorescein isothiocyanate- and Cy3-conjugated monoclonal antibodies (mAbs) that were di
115 from Xenopus embryos 1 d after injection of Cy3-conjugated tubulin into one of the blastomeres of tw
116 g carcinoma cell line A549 was infected with Cy3-conjugated viruses for 10 min followed by a 1-h incu
118 to an E. coli 30S initiation complex (30SIC(Cy3)) containing Cy3-labeled initiation factor 2 complex
120 We have now applied single-molecule FRET to Cy3, Cy5 double-labeled LacI-DNA loops diffusing freely
121 the visible fluorophores (e.g., fluorescein, Cy3, Cy5), it should find broad applicability in FRET as
122 lative fluorescence of DNAs labeled with the Cy3, Cy5, Alexa Fluor 555, and Alexa Fluor 647 dyes and
124 ye labels (ROX, rhodamine 6G, HEX, FAM, TET, Cy3, Cy5, TAMRA) attached to oligonucleotide strands is
127 rs (TMR-ATTO647N, Cy3-ATTO647N, TMR-Cy5, and Cy3-Cy5), we find that this phenomenon depends on the na
129 Here we show that these rigidly inserted Cy3/Cy5 chromophores also exhibit two additional useful
130 primer-template DNA constructs labeled with Cy3/Cy5 donor-acceptor Forster resonance energy transfer
132 hese measurements probe the distance between Cy3/Cy5 fluorophores that label the ends of a short (15-
134 ling the three stems of the DNAzyme with the Cy3/Cy5 FRET pair two stems at a time in order to gain d
137 or-acceptor pairs, fluorescein/rhodamine and Cy3/Cy5, as a function of peptide concentration and dono
138 ingle-molecule FRET (smFRET) measurements on Cy3/Cy5-labeled primer-template (p/t) DNA constructs in
139 Proteins are covalently labeled with Cy2 and Cy3 DIGE and detected simultaneously with a rotary confo
140 s indicate that fluorescence is modulated by Cy3-DNA interactions in a sequence-dependent manner.
142 rescence resonance energy transfer between a Cy3 donor and Cy5 acceptor fluorescent pair placed on op
143 (FRET) between a pair of fluorescent probes (Cy3 donor and Cy5 acceptor) placed on opposite sides of
144 y acceptor photobleaching, works for the GFP-Cy3 donor-acceptor pair and allows the full quantitation
145 By labeling three arms of the junction with Cy3 (donor), Cy5 (acceptor 1), and Cy5.5 (acceptor 2), d
146 fficient intramolecular energy transfer from Cy3 donors to stoichiometrically amplify the fluorescenc
147 ong-lived Cy5 dark state in conjunction with Cy3 donors, we demonstrate image extraction from a large
148 med dyedrons) comprised of multiple cyanine (Cy3) donors coupled to a single malachite green (MG) acc
149 and genomic DNAs with fluorescently labeled Cy3-dUTP and potentially be useful for diagnostic applic
150 e colors of the crystals by attaching either Cy3 dye (pink) or Cy5 dye (blue-green) to the components
151 ion fragments originating from probe DNA and Cy3 dye are enriched in microspot centers, correlating w
155 printed DNA distributions by tracking bound Cy3 dye, DNA base, and phosphate specific ion fragments
156 perties of dye-conjugated FDNs (Cy5-FDNs and Cy3-FDNs) were characterized using single-molecule fluor
159 The time-courses obtained by monitoring Cy3 fluorescence display a distinct lag phase that incre
163 ilaments were labeled with an amino-specific Cy3 fluorescent dye and were visualized on a quartz slid
164 ber) by conjugating Ad capsids directly with Cy3 fluorescent dye, permitting the trafficking of the c
165 ed a single head of the kinesin dimer with a Cy3 fluorophore and localized the position of the dye to
166 nd whose substrate strand is modified with a Cy3 fluorophore at the 5' end and a molecular quencher a
168 t fluorescence lifetime analysis of a single Cy3 fluorophore attached to the promoter region of the D
170 therefore been used between fluorescein and Cy3 fluorophores attached to the ends of helical arms to
175 pproximately 10(8) M(-1)s(-1)) observed with Cy3-Hfq was followed by a slow transition (0.5 s(-1)) to
176 Its high specificity makes (Ni(2+)-NTA)(2)-Cy3 ideal for detecting His(6) proteins in complex mixtu
178 An increase in fluorescence intensity of Cy3 is observed at the single-molecule level, reflecting
179 e absence of uranyl, the fluorescence of the Cy3 is quenched by both AuNP and the molecular quencher.
180 Electrokinetic injection of fluorescent dye (Cy3) labeled oligonucleotide target into a microfluidic
184 luorescently labeled by exchange of a single Cy3-labeled calmodulin into the neck region of one head.
188 FL-labeled ferritin at the membrane, whereas Cy3-labeled ferritin was found both at the membrane and
189 showed effective tumor-targeting by a novel Cy3-labeled folic acid (FA)-derivatized DNA dendrimer na
190 ere we show that SP-D enhances the uptake of Cy3-labeled fragments of DNA and DNA-coated beads by U93
191 n cultured endothelial cells (ECs), TA145, a cy3-labeled homologue of RP748, localized to alpha(v)bet
193 S initiation complex (30SIC(Cy3)) containing Cy3-labeled initiation factor 2 complexed with GTP leads
194 were characterized using smFRET between the Cy3-labeled L1 stalk of the large ribosomal subunit and
195 of all four native nucleotides and a set of Cy3-labeled nucleotides by the Klenow fragment of Escher
197 rnalization of anionic liposome-encapsulated Cy3-labeled oligonucleotides (AL-Cy3ONs) by hippocampal
198 Anionic liposome-mediated internalization of Cy3-labeled oligonucleotides by neurons and several othe
199 ergy transfer (FRET) between (Ni(2+)-NTA)(2)-Cy3-labeled proteins and suitable donors/acceptors provi
200 h1 antibody, intracellular trafficking using Cy3-labeled rAAV2, and blocking assays for proteasome an
201 DNA tails was examined by competition with a Cy3-labeled reference DNA which undergoes a Cy3 fluoresc
203 synthesis, using ribosomes containing either Cy3-labeled ribosomal protein L11 and A- or P-site Cy5-l
204 almonella was performed and visualized using Cy3-labeled secondary probes in a sandwich-type assay fo
205 (Ec) SSB is bound to surface-immobilized 3'-Cy3-labeled ssDNA, a fluctuating FRET signal is observed
209 tissue pretreatment and hybridization with a Cy3-labeled telomeric repeat complementing (CCCTAA)3 pep
213 ffects on spot drying, with high-density DNA-Cy3 located in spot centers and nonhomogeneous DNA distr
215 the loading strand, the enzyme displaces the Cy3-modified reporter, which will bind to a capture or c
216 luorescence photobleaching recovery, using a Cy3-modified, noncompetitive mAb specific for IL-1RI (M5
217 ion of tissue sections in mice receiving the Cy3-MORF/tat/trastuzumab nanoparticle showed evidence of
218 -terminus-KS-AT-MT1-KR-ACP) one NRPS module (Cy3-MT2-PCP3-TE-C-terminus)], was used as a model system
219 different conformation, from that of either Cy3 or Cy5 attached by 3-atom tethers, with the long axe
221 al properties of DNA oligomers, labeled with Cy3 or Cy5, when bound to quartz surfaces coated with me
223 r fluorophore Cy5, while donor fluorophores (Cy3 or EPI) were attached to HPMA copolymer side chains
224 the fluorescence intensity of fluorophores (Cy3 or fluorescein) attached to the 5'-end of a series o
226 by a relative intensity analysis of the red (Cy3 photoluminescence (PL)) and green (gQD PL) color cha
228 ding to DNA molecules sparsely labelled with Cy3 results in an increase in fluorescence intensity due
229 led with 3,3'-Diethyloxacarbocyanine iodide (CY3), Rhodamine 6G (R6G), and 3,3'-Diethylthiadicarbocya
230 elf-complementary DNA duplex 5'-labeled with Cy3 shows that the fluorophore is stacked onto the end o
231 teraction with the target as the fluorescent Cy3 signal is quenched by the gold nanoparticle and only
232 ithms, allowed the spectral contributions of Cy3 signal, glass, and contaminating fluorescence to be
233 ckground array signal, normalize the Cy5 and Cy3 signals, score levels of differential hybridization,
234 g glomerular filtration, endocytic uptake of Cy3-siRNA by PTCs was rapid and extensive, and significa
236 The simulations also showed that stacking of Cy3 straightens the DNA while stabilizing the first base
237 ed material for electron microscopy and with Cy3-streptavidin to label the neurons with fluorescence
238 exes terminally labeled with fluorescein and Cy3 suggests that the Cy3 is close to the helical axis o
239 sfer (FRET) between GFP-tagged KIR2DL1 and a Cy3-tagged generic anti-phosphotyrosine monoclonal antib
240 This is very similar to the behavior of Cy3 terminally attached to DNA and suggests that the eff
241 n increase in the fluorescence anisotropy of Cy3 terminally attached to one of the helical arms of a
242 nert) to quench the triplet excited state of Cy3, the most ubiquitous green emissive dye utilized in
243 IP-1 links two fluorophores (fluorescein and Cy3) through an Fe(II)-cleavable endoperoxide bridge, wh
245 nfocal microscopy revealed colocalization of Cy3-tilmanocept with the macrophage membrane MR and bind
246 of other proteins, allowing (Ni(2+)-NTA)(2)-Cy3 to be used as a probe in crude cell extracts and as
247 scence resonance energy transfer (FRET) from Cy3 to Cy5 since the two ends of (dT)(66) are in close p
248 rgy transfer (smFRET) between (Cy5)EF-G and (Cy3)tRNALys, we studied the translational elongation dyn
251 ency increased when the solution of AMCA-DNA-Cy3 was placed between two quartz plates coated with sil
252 were adsorbed onto a cellulose support, and Cy3 was used as a green channel acceptor for Survival Mo
255 idine tail and labeled with an acceptor dye (Cy3) was immobilized on the nanocrystals via a noncovale
256 denovirus, labeled with the fluorescent dye (Cy3), was rapidly and widely distributed on epithelial s
258 One reporter is modified with cyanine 3 (Cy3), whereas the other is modified with a spectrally pa
259 the common single molecule fluorescence dye Cy3, which is highly susceptible to this destructive pat
261 ised tissues via conjugation of streptavidin-Cy3, which targeted the probe biotin moiety, and immunoh
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