ライフサイエンスコーパス: Cy5

1 colour channel opposite of their design (Cy3/Cy5).

2 t tissues using a fluorescent peptide (RK-10-Cy5).

3 idoamine) dendrimer nanoparticles (Dendrimer-Cy5).

4 with donor (Cy3) and acceptor fluorophores (Cy5).

5 BLM, and BLM disaccharide to the cyanine dye Cy5**.

6 accharide was doubly labeled with biotin and Cy5.

7 e quantification of cell-extracted dendrimer-Cy5.

8 re labeled with fluorophores such as Cy3 and Cy5.

9 es commonly used in DNA microarrays, Cy3 and Cy5.

10 containing RNAs with the fluorophores Cy3 or Cy5.

11 deceased MM patients were stained with LLP2A-Cy5.

12 es was more than tripled compared to FAM and Cy5.

13 onjugated with one of the FRET pair, Cy3 and Cy5.

14 both nuclear and optical in vivo imaging of Cy5-(111)In -DTPA-Tyr(3)-octreotate were performed in NE

15 dies revealed similar tumor uptake values of Cy5-(111)In-DTPA-Tyr(3)-octreotate and (111)In -DTPA-Tyr

16 en apoCph1Delta and the red fluorescent dyes Cy5.18 and Atto 655 but not Alexa Fluor 660.

17 efficiencies between the juxtaposed Cy3 and Cy5 5'-end labeled viral DNA ends in the synaptic comple

18 MRI is only slightly lower than that of ZD2-Cy5.5 (0.5 micromol kg(-1)) in fluorescence imaging.

19 tion with Cy3 (donor), Cy5 (acceptor 1), and Cy5.5 (acceptor 2), distance changes between the donor a

20 beacon (rMB), thus bringing Cy5 (donor) and Cy5.5 (acceptor) into close proximity to allow fluoresce

21 nt normal tissue, we developed a chlorotoxin:Cy5.5 (CTX:Cy5.5) bioconjugate that emits near-IR fluore

22 that had been site specifically labeled with Cy5.5 (scVEGF/Cy) or inactivated scVEGF/Cy (inVEGF/Cy) a

23 Epo-Cy5.5 allows the longitudinal analysis of EpoR expressio

24 optical probes based on Igs conjugated with Cy5.5 and Cy7 and demonstrate in animal models that thes

25 oclonal antibody C225 or EGF followed by EGF-Cy5.5 and examined under a fluorescence microscope.

26 njugate arginine-glycine-aspartic acid (RGD)-Cy5.5 as a contrast agent in vitro, in vivo, and ex vivo

27 ologic blocker of MMP-2 and induction of CTX:Cy5.5 binding in MCF-7 cells following transfection with

28 e-2 (MMP-2) as evidenced by reduction of CTX:Cy5.5 binding in vitro and in vivo by a pharmacologic bl

29 Vessel density and Epo-Cy5.5 binding on endothelial cells were comparable.

30 of the magnetofluorescent nanoparticle CLIO-Cy5.5 by macrophages in infarcted myocardium was studied

31 The uptake of CLIO-Cy5.5 by macrophages infiltrating the infarcted myocardi

32 n vivo optical imaging using the cyanine dye Cy5.5 conjugate.

33 Tumor accumulation of Epo-Cy5.5 could be significantly reduced by adding unlabeled

34 nance energy transfer (FRET) between Cy5 and Cy5.5 double-dye terminated DNAs.

35 or magnetic resonance imaging, modified with Cy5.5 dye (for near-IR fluorescence optical imaging), an

36 e to a larger overlap between the ODN-linked Cy5.5 emission and Cy7 excitation spectra.

37 ed in p50 binding and measurable increase of Cy5.5 emission.

38 The RGD-Cy5.5 exhibited intermediate affinity for alpha(v)beta(3

39 ICG and Cy5.5 fluorescence was completely absent from the tumor

40 als (86Y or 111In) and the near-infrared dye Cy5.5 for dual modality PET (or SPECT) and fluorescence

41 in B domain (CTB) for RGC-targeting and with Cy5.5 for unimNP-tracing.

42 nce intensity in mice confirmed that ICG and Cy5.5 had no favorable binding to tumor regardless of EG

43 Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profile

44 These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraop

45 y available NIR fluorophores IRDye800-CW and Cy5.5 in vitro for immunocytometry, histopathology and i

46 The reporter duplexes included donor NIR Cy5.5 indodicarbocyanine fluorochrome linked to the 3' e

47 limeter and detection limit for fluorochrome Cy5.5 of 1-10 pmol.

48 by trypsin, was synthesized by labeling with Cy5.5 of a pegylated poly-L-lysine copolymer.

49 ging agents, we site-specifically conjugated Cy5.5 or (64)Cu-1,4,7,10-tetra-azacyclododecane-N,N',N''

50 EGF-Cy5.5, but not Cy5.5 or indocyanine green dye (ICG), bound to MDA-MB-46

51 pancreata of mice injected with the MN-Ex10-Cy5.5 probe compared with control animals injected with

52 In contrast, binding of EGF-Cy5.5 to MDA-MB-435 cells was not observed.

53 Epo-Cy5.5 was generated by coupling Cy5.5 to rhuEpo.

54 validation confirmed specific binding of Epo-Cy5.5 to the tumor cells, and this binding correlated wi

55 Moreover, tumor uptake of EGF-Cy5.5 was blocked by C225.

56 Tumor uptake of RGD-Cy5.5 was blocked by unlabeled c(RGDyK).

57 In vivo specificity of Epo-Cy5.5 was confirmed by competition analyses using micro-

58 Almost constant accumulation of Epo-Cy5.5 was found in bone marrow and tumors, indicating sp

59 Epo-Cy5.5 was generated by coupling Cy5.5 to rhuEpo.

60 betic and diabetic NOD mice injected with MN-Cy5.5 was not significantly changed, reflecting the nons

61 for Survival Motor Neuron (SMN1) target, and Cy5.5 was the red channel acceptor for the glucuronidase

62 Binding of Epo-Cy5.5 was validated on tumor cryosections.

63 issue, we developed a chlorotoxin:Cy5.5 (CTX:Cy5.5) bioconjugate that emits near-IR fluorescent signa

64 lation of the targeted probe (termed MN-Ex10-Cy5.5) compared with nontargeted (termed MN-Cy5.5).

65 end-labeled with a donor (Cy5) or acceptor (Cy5.5) dye.

66 -Cy5.5) compared with nontargeted (termed MN-Cy5.5).

67 odality nanoparticles using glycol chitosan, Cy5.5, and superparamagnetic iron oxide nanoparticles (S

68 EGF-Cy5.5, but not Cy5.5 or indocyanine green dye (ICG), bou

69 435 (EGFr-) cancer cells were incubated with Cy5.5, EGF-Cy5.5, or the anti-EGFr monoclonal antibody C

70 etic NOD mice after the injection of MN-Ex10-Cy5.5, indicating the decrease of probe accumulation in

71 cancer cells were incubated with Cy5.5, EGF-Cy5.5, or the anti-EGFr monoclonal antibody C225 or EGF

72 fer (FRET) both in the case of Cy5.5-Cy7 and Cy5.5-800CW pairs of fluorochromes, which was sensitive

73 e energy transfer (FRET) both in the case of Cy5.5-Cy7 and Cy5.5-800CW pairs of fluorochromes, which

74 gher FRET efficiency observed in the case of Cy5.5-Cy7 pair was due to a larger overlap between the O

75 were able to visualize in vivo binding of a Cy5.5-labeled peptide specific for EGFR to the cell surf

76 n myocardial remodeling using a radiolabeled Cy5.5-RGD imaging peptide (CRIP) that targets myofibrobl

77 5-superparamagnetic iron oxide nanoparticle (Cy5.5-SPION) labeling and fluorescent microscopy, we dem

78 may/J] that had been administered AS-gfap or Cy5.5-SPION-gfap.

79 ratios of 5.1 with IRDye800-CW and 2.7 with Cy5.5.

80 s that can be labeled with near-infrared dye Cy5.5.

81 with 3 to 20 mg of iron per kilogram of CLIO-Cy5.5.

82 i.v. with C225 24 h before injection of EGF-Cy5.5.

83 nce donor, while the endo V was labeled with Cy5, a fluorescence acceptor.

84 ance energy transfer between a Cy3 donor and Cy5 acceptor fluorescent pair placed on opposite sides o

85 a pair of fluorescent probes (Cy3 donor and Cy5 acceptor) placed on opposite sides of a nick in dupl

86 dot (QD) streptavidin conjugate donor and a Cy5 acceptor.

87 three arms of the junction with Cy3 (donor), Cy5 (acceptor 1), and Cy5.5 (acceptor 2), distance chang

88 e fluorescence of DNAs labeled with the Cy3, Cy5, Alexa Fluor 555, and Alexa Fluor 647 dyes and by (i

89 ission wavelengths of the two labels Cy3 and Cy5 allow for fluorescence resonance energy transfer in

90 frequency and duration of blinking events of Cy5, an effect that scales with reducing potential.

91 wed lower maximal MEF factors of 10-fold for Cy5 and 2.5-fold for Cy3, because of the smaller amount

92 emained at a constant maximum of 28-fold for Cy5 and 4-fold for Cy3, compared to avidin-coated glass

93 tobleaching compared to organic dyes such as Cy5 and Alexa 647 in vitro, and 5-fold more photons comp

94 t the background array signal, normalize the Cy5 and Cy3 signals, score levels of differential hybrid

95 en the two fluorophores used in aCGH-usually Cy5 and Cy3-can be observed as a bias within the intensi

96 nce resonance energy transfer (FRET) between Cy5 and Cy5.5 double-dye terminated DNAs.

97 ition, the binding curves obtained with 8A11-Cy5 and DCP-UO22+ species changed from sigmoidal to hype

98 ingle injection of ACPPD dually labeled with Cy5 and gadolinium chelates enabled preoperative whole-b

99 nce energy transfer (FRET) between 605QD and Cy5 and Iowa Black RQ, we develop a single-QD-based apta

100 re coincubated with the optical analog LLP2A-Cy5 and mouse B220, CD4, Gr1, and Mac1 antibodies and an

101 We report that the cyanine dye Cy5 and several of its structural relatives are reversib

102 rophore (Cy5), and the FRET-coupling between Cy5 and the redox center (copper) was used to study ET t

103 conjugation with a fluorescent cyanine dye (Cy5) and biotin, resulting in binding K(i) values of 17

104 resonance energy transfer to a reporter dye (Cy5) and that can benefit from the light harvesting prop

105 In one approach, multiple organic dyes (Cy5) and Trolox molecules are randomly distributed on de

106 es were labeled with an organic fluorophore (Cy5), and the FRET-coupling between Cy5 and the redox ce

107 d increased initial lifetimes of single Cy3, Cy5, and Alexa488 fluorophores.

108 le, Cy3, and fluorescence acceptor molecule, Cy5, and by varying the concentration of NgoMIV endonucl

109 r dye pairs (TMR-ATTO647N, Cy3-ATTO647N, TMR-Cy5, and Cy3-Cy5), we find that this phenomenon depends

110 rization and back-isomerization processes of Cy5, and Cy5-nucleobase interactions are shown to slow d

111 imately 540 nm), Cy3 (approximately 570 nm), Cy5 (approximately 670 nm), IRDye 680 (approximately 700

112 The indodicarbocyanine fluorophores Cy3 and Cy5 are extensively used as donor-acceptor pairs in fluo

113 The observed FBKs of Cy5 are found to predominantly originate from the isomer

114 he data show that Cy-ncAAs (based on Cy3 and Cy5) are tolerated by the eukaryotic ribosome in cell-fr

115 Using Cy5 as a model, we show that the quenching reaction occu

116 ble cell-penetrating peptides, which contain Cy5 as far red fluorescent donor and Cy7 as near-infrare

117 cceptor pairs, fluorescein/rhodamine and Cy3/Cy5, as a function of peptide concentration and donor-to

118 ent conformation, from that of either Cy3 or Cy5 attached by 3-atom tethers, with the long axes of th

119 of the indocarbocyanine fluorophores Cy3 and Cy5 attached to DNA via three-carbon atom tethers, showi

120 Cy5-avidin conjugate-bound silver nanoparticles were pre

121 by in-gel fluorescence after conjugation to Cy5-azide.

122 nzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and im

123 or accumulation of dibenzocyclooctyne (DBCO)-Cy5 by subsequent click chemistry.

124 e fluorescent signal of the carbocyanine dye Cy5 by using an engineered virus as a scaffold to attach

125 We found that the FBKs of Cy5 can be tuned by having more or less unpaired guanine

126 d be discriminated with equal confidence for Cy5 channel and reduced levels of confidence (>68%) for

127 Here we show that these rigidly inserted Cy3/Cy5 chromophores also exhibit two additional useful prop

128 ; labeled with Cy3) and acidic (labeled with Cy5) conditions.

129 e 8A11-Cy5 conjugate; incubation of the 8A11-Cy5 conjugate with saturating concentrations of DCP-UO22

130 l of protein G bind to each mole of the 8A11-Cy5 conjugate, (ii) binding of the first equivalent of D

131 fect on the binding of protein G to the 8A11-Cy5 conjugate; incubation of the 8A11-Cy5 conjugate with

132 asures intensity of Bcl-2 expression using a Cy5 conjugated antibody within the mask.

133 sured HSP90 expression within the mask using Cy5-conjugated antibodies.

134 Cy5-conjugated miR-146a and miR-181b were packaged in po

135 The synthesized Cy5-conjugated MUB(70) marker specifically stained the c

136 kness of about 9mum while it was 40ng/mL for Cy5-conjugated Rabbit Anti-Mouse IgG which is 2.5x10(5)

137 ed for d-(+)-glucose and 96cm(-1)/mug/mL for Cy5-conjungated Rabbit Anti-Mouse IgG.

138 tion of an Escherichia coli 50S subunit (50S(Cy5)) containing a Cy5-labeled L11 N-terminal domain (L1

139 40-fold and triggers tissue retention of the Cy5-containing fragment.

140 Conversely, incubation of the 8A11-Cy5 covalent conjugate with saturating concentrations of

141 ommonly used fluorophore indocarbocyanine-5 (Cy5) covalently attached to the 5'-terminus of double-he

142 Such cleavage increases the Cy5:Cy7 emission ratio 40-fold and triggers tissue reten

143 [(89)Zr]Zr-DFO- and Cy5-daratumumab demonstrated superb binding to CD38(+) h

144 Using the long-lived Cy5 dark state in conjunction with Cy3 donors, we demons

145 The method employs Cy5-dATP incorporation into a DNA primer that has been p

146 (64)Cu-LLP2A and LLP2A-Cy5 demonstrated binding specificity for VLA-4 in an imm

147 (64)Cu-LLP2A and LLP2A-Cy5 demonstrated high specificity for VLA-4-positive mou

148 Confocal microscopy with daratumumab-Cy5 demonstrated specific cell binding.

149 rease in emission lifetimes as compared with Cy5-DNA free molecules in the absence of metal.

150 the Cy5-DNA-Ag particle as compared to free Cy5-DNA resulted in an increased contribution of Cy5-DNA

151 The decrease of lifetime for the Cy5-DNA-Ag particle allowed us to resolve the correlatio

152 The increased brightness of the Cy5-DNA-Ag particle as compared to free Cy5-DNA resulted

153 The single Cy5-DNA-Ag particles showed more than 10-fold increase i

154 DNA resulted in an increased contribution of Cy5-DNA-Ag to the correlation function of the mixture.

155 ermediates of motors containing Cy3-pRNA and Cy5-DNA.

156 On this account, the adsorption behaviour of Cy5-dNTPs on a variety of surface coatings was studied b

157 mer-template DNA constructs labeled with Cy3/Cy5 donor-acceptor Forster resonance energy transfer (FR

158 everse molecular beacon (rMB), thus bringing Cy5 (donor) and Cy5.5 (acceptor) into close proximity to

159 , and 3,3'-Diethylthiadicarbocyanine iodide (CY5)-doped polymer as the reconfigurable gain media.

160 demonstrated in this work by using Cy3- and Cy5-doped nanoparticles in sandwich hybridization.

161 ave now applied single-molecule FRET to Cy3, Cy5 double-labeled LacI-DNA loops diffusing freely in so

162 l (NBA) or Trolox to the cyanine fluorophore Cy5 dramatically enhanced fluorophore photostability wit

163 tudy, the hybrid labeled somatostatin analog Cy5-DTPA-Tyr(3)-octreotate (DTPA is diethylene triamine

164 ing affinity and internalization capacity of Cy5-DTPA-Tyr(3)-octreotate were assessed in vitro.

165 ion constant value was 387.7 +/- 97.9 nM for Cy5-DTPA-Tyr(3)-octreotate, whereas it was 120.5 +/- 18.

166 treotate to 1.32% +/- 0.02% applied dose for Cy5-DTPA-Tyr(3)-octreotate.

167 ceptor affinity and internalization rate for Cy5-DTPA-Tyr(3)-octreotate.

168 ystals by attaching either Cy3 dye (pink) or Cy5 dye (blue-green) to the components of the crystal, y

169 were explored in Caco-2 cells by fluorescent Cy5 dye as a hydrophobic drug model.

170 ng the fluorescence of a covalently attached Cy5 dye label whose fluorescence is (partly) quenched by

171 ye pair was associated with high variance in Cy5 dye signals.

172 Cy3 or Cy5 dye-labeled DNA exhibited reduced fluorescence and a

173 , but are spectrally compatible with Cy3 and Cy5 dye-labeled targets when using confocal laser scanne

174 guanines (upG) and thymines (upT) around the Cy5 dye.

175 Daratumumab was conjugated to Cyanine5 (Cy5) dye for cell microscopy.

176 hiometric amounts, and in a second approach, Cy5 dyes are covalently linked to Trolox in a precise 1:

177 ling and hybridization efficiency of Cy3 and Cy5 dyes.

178 resonance energy transfer (smFRET) between (Cy5)EF-G and (Cy3)tRNALys, we studied the translational

179 nal light-sheet fluorescence microscopy with Cy5-Fab-PAS200 confirmed better tracer extravasation in

180 herefore resulting in a measurable change in Cy5 FBKs.

181 ophysical properties of dye-conjugated FDNs (Cy5-FDNs and Cy3-FDNs) were characterized using single-m

182 In addition, Cy5-FDNs were used as reporter probes in a single-molecu

183 As proof-of-principle demonstration, Cy5-FDNs were used to assay single-molecule nucleic acid

184 Fluorophore Cy3/Cy5 fluorescence intensities were analyzed both through

185 The signal from the Cy5 fluorescence probe shows additional effects that app

186 nally, we observed specific quenching of the Cy5 fluorescent dye when the FeS cluster of a bound heli

187 The RA twin cDNA was labeled with cy5 fluorescent dye, and the cDNA of the healthy co-twin

188 licase is positioned in close proximity to a Cy5 fluorophore incorporated into the DNA molecule.

189 i.e., TH peptide), and imaging probes (i.e., Cy5 fluorophore).

190 ons from rat liver as a source of ASGP-R and Cy5 fluorophore-labeled triGalNAc synthetic ligand as a

191 nce of a background of hundreds of nanomolar Cy5 fluorophore.

192 measurements probe the distance between Cy3/Cy5 fluorophores that label the ends of a short (15-nt)

193 We use the normal Cy5 FRET acceptor to observe arrival of a fluorescently

194 ophilic 3'-phosphorothioate probe carrying a Cy5 FRET acceptor, and an electrophilic probe containing

195 Using Cy3/Cy5 FRET efficiency to monitor binding of Mg(2+), we sho

196 the three stems of the DNAzyme with the Cy3/Cy5 FRET pair two stems at a time in order to gain deepe

197 Global analysis of the Cy3 and Cy5 FRET time-courses, using an n-step sequential DNA un

198 The 30SIC(Cy3):50S(Cy5) FRET signal also provides a sensitive probe of the

199 l penetrating peptides (ACPPs), labeled with Cy5, gadolinium, or both.

200 in vivo and validated neuronal uptake using Cy5-goat IgPxcIgY ex vivo.

201 Photoinduced blinking of Cy5 has hampered many previous investigations using this

202 y transfer efficiency, with unlinked QDs and Cy5 hydrazide producing nearly zero background signal in

203 nd to bleach significantly less than FAM and Cy5; i.e., the final fluorescence of Atto dyes was more

204 med by measuring the ratio of bound and free Cy5-insulin and FITC-glucagon in the presence of their r

205 laser was pulsed at 73 Hz and used to excite Cy5-insulin, while a vertically polarized 488 nm laser p

206 DDS dramatically enhanced cellular uptake of Cy5 into Caco-2 cells.

207 We find that Cy5 is predominantly stacked onto the end of the duplex,

208 Cy5 is quenched in favor of Cy7 re-emission until the in

209 isible fluorophores (e.g., fluorescein, Cy3, Cy5), it should find broad applicability in FRET assays

210 Furthermore, the sulfo-Cy5 labeled (R,R)-14 retained high agonist potency as a

211 We demonstrate ITP-AC with 25 nt, Cy5 labeled DNA target and a DNA probe and study the spa

212 f concept, we demonstrate the interaction of Cy5 labeled IgG and Alexa633 labeled anti-IgG using a si

213 fluorescently stained plasmid DNA and single Cy5 labeled oligonucleotides.

214 r the dynamics of fluorescence emission from Cy5 labeled on DNA probes.

215 rescent protein show colocalization with the Cy5-labeled ACPPs.

216 the sliding via one-dimensional diffusion of Cy5-labeled adenovirus protease on DNA in the presence o

217 ing an increase in fluorescent intensity for Cy5-labeled analytes measured with a confocal microarray

218 fferences in the detection limits of Cy3 and Cy5-labeled cDNA, a potential concern for array data on

219 Diffusion-distance estimates for Cy3- and Cy5-labeled cDNAs were 3.8 mm and 2.6 mm, respectively,

220 strogen receptor, at the same time causing a Cy5-labeled coactivator to be recruited to the estrogen

221 50-nm-diameter single silver particle, and a Cy5-labeled complementary single-stranded oligonucleotid

222 Following systemic administration of the Cy5-labeled dendrimer (D-Cy5), we demonstrate dendrimer

223 Cy5-labeled DNA aptamer was embedded between the Au and

224 When Cy5-labeled Escherichia coli (Ec) SSB is bound to surfac

225 Pairs of cy3- and cy5-labeled extracts from 5-week old Alport and wild-typ

226 ocalization experiments were performed using Cy5-labeled forked DNA and Alexa 555-labeled gp59 in the

227 In the same mouse models, Cy5-labeled free ACPPs and ACPPs conjugated to dendrimer

228 luorescent quenching graphene oxide (GO) and Cy5-labeled G8 aptamer were used in this study to develo

229 Detection limits in the analysis of Cy5-labeled IgG are 0.02 ng/mL because of the high surfa

230 by electroosmotic flow and mixed online with Cy5-labeled insulin, fluorescein isothiocyanate (FITC)-l

231 erve the sequence-independent interaction of Cy5-labeled interferon-inducible protein 16 with DNA and

232 hia coli 50S subunit (50S(Cy5)) containing a Cy5-labeled L11 N-terminal domain (L11-NTD) within the G

233 fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distances from t

234 l freedom, and dwell time distributions of a Cy5-labeled OTD (L1Cy5-7OTD) as it interacted with sever

235 t all the click clusters efficiently deliver Cy5-labeled pDNA into HeLa and H9c2 (2-1) cells, and com

236 e-molecule FRET (smFRET) measurements on Cy3/Cy5-labeled primer-template (p/t) DNA constructs in the

237 By loading a high density of Cy5-labeled probe DNA on microspheres (15 mum), we achie

238 abeled by pre-hybridization to complementary Cy5-labeled probes.

239 to the patterned antigens was revealed using Cy5-labeled rabbit anti-mouse IgG.

240 t whereby immobilized L7Ae protein binds Cy3-Cy5-labeled RNA from free solution.

241 dified biotin was immobilized for binding of Cy5-labeled streptavidin.

242 beled ribosomal protein L11 and A- or P-site Cy5-labeled tRNA or Cy3- and Cy5-labeled tRNAs.

243 1 stalk of the large ribosomal subunit and a Cy5-labeled tRNA(Lys) in the ribosomal peptidyl-tRNA-bin

244 nd A- or P-site Cy5-labeled tRNA or Cy3- and Cy5-labeled tRNAs.

245 Cy5-labeled, heat-killed cells were used to demonstrate

246 ed from the same source was used for Cy3 and Cy5 labeling and hybridized to the same array, signal in

247 FAM-labeled ATP aptamer and Cy5-modified GTP aptamer are used to construct the multi

248 ion and reduction processes of individual Az-Cy5 molecules were monitored.

249 Hence, Cy5-MUB(70) is a novel and specific fluorescent marker f

250 and back-isomerization processes of Cy5, and Cy5-nucleobase interactions are shown to slow down these

251 llography to analyze the location of Cy3 and Cy5 on dsRNA, using complexes of an RNA stem-loop bound

252 her show that the alterations of the FBKs of Cy5 on probe hybridization can be used to differentiate

253 n of 8A11 with amine-reactive derivatives of Cy5 or Alexa 488 altered the binding curves obtained wit

254 rRNA gene and were end-labeled with a donor (Cy5) or acceptor (Cy5.5) dye.

255 egion (Cy2) and 8000-fold in the red region (Cy5) over conventional state-of-the-art image acquisitio

256 nti-TRPC protein antibodies labeled with cy3-cy5 pairs.

257 RK-10-Cy5 peptide also demonstrates PD-L1 detection in NSCLC,

258 ortion of cells intensely labeled with LLP2A-Cy5 probe.

259 Taking advantage of this Cy5 quenching, we developed an equilibrium assay for ana

260 s detections of two target DNAs with FAM and Cy5 reporter conjugated probes.

261 engineered virus as a scaffold to attach >40 Cy5 reporter molecules at fixed locations on the viral c

262 trate that organized spatial distribution of Cy5 reporter molecules on the capsid obviates this commo

263 functionalized with QDs and a dye acceptor (Cy5), respectively (detection limit 12 nM).

264 Cy5-RO5323441 was injected to study the intratumor distr

265 Cy5-RO5323441 was present in the tumors mainly in the mi

266 the dye pair used, with the cyanine pair Cy3-Cy5 showing the least amount of fluctuations.

267 However, no change was observed in the Cy3/Cy5 signal intensity ratio between continuously mixed an

268 ltiplier tubes for detection of the FITC and Cy5 signal.

269 A total of 401 genes with Cy3 and Cy5 spot intensities of >/=500 were selected for analysi

270 ropy measurements indicate that both Cy3 and Cy5 stack on the ends of the RNA.

271 een phycoerythrin-biotin (PhycoE-Biotin) and Cy5-streptavidin trapped in the two proteoliposome popul

272 Synthesis of a fluorescently (Cy5) tagged C-11 fatty acid is reported.

273 binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs.

274 Hybridization is performed with Cy5-tagged single-stranded targets derived by PCR from g

275 bels (ROX, rhodamine 6G, HEX, FAM, TET, Cy3, Cy5, TAMRA) attached to oligonucleotide strands is repor

276 fluorescein and are compatible with Cy3 and Cy5 target labeling dyes when using confocal laser scann

277 fficiency of energy transfer between Cy3 and Cy5, that are attached to nucleic acids in this way, wil

278 e at the excitation wavelength of cyanine-5 (Cy5), thus providing an increase in fluorescent intensit

279 f the phosphine to the polymethine bridge of Cy5 to form a covalent adduct.

280 ed with the cyanine dye fluorophores Cy3 and Cy5 to quantify the melting/rebinding reaction by fluore

281 Attachment of Cy3, Cy3B and Cy5 to the 5-position of thymidine by an ethynyl linker

282 grams of the logarithms of the ratios of the Cy5 to the reference fluorophore fluorescence can be use

283 oxin B subunit-Alexa 647 and streptavidin-PE/Cy5, to membranes containing different amounts of ligand

284 chiometry followed by covalent attachment of Cy5-Trolox conjugates onto dendrimers.

285 d to single self-healing dye systems such as Cy5-Trolox, and as a proof-of-principle demonstration, w

286 uration of the three-way-junction alters the Cy5-upG or Cy5-upT interactions, therefore resulting in

287 the three-way-junction alters the Cy5-upG or Cy5-upT interactions, therefore resulting in a measurabl

288 rescence emission of the covalently attached Cy5 was largely quenched by FRET ('off'-state), whereas

289 ength and emission wavelength of Cyanines 5 (Cy5), was used to amplify the fluorescence signal intens

290 tation wavelength and emission wavelength of Cy5, was used to amplify the fluorescence signal intensi

291 ministration of the Cy5-labeled dendrimer (D-Cy5), we demonstrate dendrimer uptake in cells involved

292 TMR-ATTO647N, Cy3-ATTO647N, TMR-Cy5, and Cy3-Cy5), we find that this phenomenon depends on the nature

293 at the model drug (Cy3) and polymer bound to Cy5 were colocalized at an early time point before the m

294 Probes labeled with Cy3 and Cy5 were individually introduced to opposite sides of a

295 mitting at the same wavelength as cyanine 5, Cy5) were found to bleach significantly less than FAM an

296 fibres labelled with different dyes (Cy3 and Cy5) were mixed, and the distribution of dyes inserting

297 erties of DNA oligomers, labeled with Cy3 or Cy5, when bound to quartz surfaces coated with metallic

298 ckbone was labeled with acceptor fluorophore Cy5, while donor fluorophores (Cy3 or EPI) were attached

299 r, the manner of interaction of both Cy3 and Cy5 with the terminus of the dsRNA is significantly diff

300 ially for far red emitting fluorophores like Cy5, without significantly altering current microarray p