ライフサイエンスコーパス: Cys residue

1 argeted mutant encoding an altered catalytic Cys residue.

2 intracellular organic hydroperoxides through Cys residue.

3 methylation of the terminal Gtgamma subunit Cys residue.

4 t a subset of some 46 kinases contained this Cys residue.

5 in chains to a protein substrate through its Cys residue.

6 hrough a C-terminal, N-terminal, or a middle Cys residue.

7 namic disulfide formation involving multiple Cys residues.

8 prediction and classification of functional Cys residues.

9 ere observed only in the vicinity of the two Cys residues.

10 extended backbone conformation at one of the Cys residues.

11 iation does not involve conserved N-terminal Cys residues.

12 ed the Nt-acetylated Ala, Val, Ser, Thr, and Cys residues.

13 into peptides and proteins by alkylation of Cys residues.

14 -terminal tail of Oxa1L does not contain any Cys residues.

15 during evolution and by clustering of other Cys residues.

16 m, determine the solvent exposure of the two Cys residues.

17 anes is due to palmitoylation of one or more Cys residues.

18 u-217 and Lys-224, in the second mutant with Cys residues.

19 yanin (KLH) carrier protein through backbone Cys residues.

20 third Cys loop between the fourth and fifth Cys residues.

21 s, in a mutant of DGK lacking the two native Cys residues.

22 by substituting both Arg-67 and Asp-82 with Cys residues.

23 e specific for substrates bearing N-terminal Cys residues.

24 s, in one of these domains, or without these Cys residues.

25 ophore binding depend on the location of the Cys residues.

26 subunit by individually replacing them with Cys residues.

27 are formed between adjacent Nav1.2 (912/918)Cys residues.

28 and subsequent exposure of previously buried Cys residues.

29 Fe-2S] cluster coordinated by four conserved Cys residues.

30 of Trx proteins at a non-catalytic cysteine (Cys) residue.

31 serine (Ser), threonine (Thr), and cysteine (Cys) residues.

32 loop (ECL) containing 16 conserved cysteine (Cys) residues.

33 Cys residues using OxICAT revealed that 381 Cys residues (33.6%) showed >10% increased oxidations un

34 ted in a higher basal oxidation level of 338 Cys residues (41.1%).

35 nd that, after addition of a single-terminal Cys residue, a CdtB homologue from cytolethal distending

36 nd iRFP713 proteins and their mutants having Cys residues able to bind BV either in both PAS (Cys15)

37 ent with iron ligation of the "noncanonical" Cys residue across subunit interfaces of the Td SOR homo

38 ues and contain ten evolutionarily conserved Cys residues across a wide variety of species.

39 e dimer formation, suggesting that these two Cys residues act as vicinal thiols, consistent with C119

40 that a mutation, C39A, of a highly conserved Cys residue among NCS proteins, increases the apparent c

41 nase (normally 27.2 A distant) with a single Cys residue and introduce new termini at a surface loop,

42 l death, which required intact extracellular Cys residues and a conserved kinase active site.

43 PLP1s is active, and the predicted catalytic Cys residues and cleavage sites for each PLP1 were confi

44 The functionality of the predicted catalytic Cys residues and cleavage sites was tested by analysis o

45 Titration of reduced Cys residues and comparative mass spectrometry on AtSBP1

46 onsistent with cluster coordination by three Cys residues and one oxygen-containing residue, and anal

47 Other GAF domain proteins substitute the Cys residues and others to specifically bind cyclic nucl

48 stored through the introduction of a pair of Cys residues and the subsequent formation of a disulfide

49 econdary structure, accessibility of Trp and Cys residues, and aggregation), in plasma membranes as w

50 disulfide bond arrangement of the conserved Cys residues, and it also extends the structural knowled

51 ylation or disulfide bridges involving these Cys residues appear to be required for ER exit of APP.

52 odified by BEL treatment, but most iPLA2beta Cys residues are alkylated at various BEL concentrations

53 Since the ten Cys residues are highly conserved in Meteorin and Cometi

54 The conserved RecB motif and four Cys residues are important for DNA binding and cleavage

55 or the functional defects when the conserved Cys residues are mutated.

56 indicating that the microenvironments of the Cys residues are mutually interdependent.

57 Further, these Cys residues are not palmitoylated in Rpe65 by mass spec

58 However, in the control cells, these Cys residues are occupied and produced less labeling wit

59 Eight additional Cys residues are oxidized at high versus low pO2 only wh

60 eptide, HD5[Ser(hexa)], where all six native Cys residues are replaced by Ser residues, was also eval

61 l as other lecithin:acyltransferases wherein Cys residues are required for catalysis.

62 ytochrome c and the identity of their active Cys residues are unknown.

63 fold and a catalytic triad (or dyad) with a Cys residue as a nucleophile.

64 a long loop between helices I and II, and a Cys residue as a quencher for the acceptor.

65 d in detail the role of the highly conserved Cys residue as well as the geometry and stereochemistry

66 n analyzing a K14 variant harboring a single Cys residue at position 367.

67 s of this protein with a specifically placed Cys residue at position 4, 39, 67, or 94 of Oxa1L-CTT ha

68 at the cytoplasmic end of TM VI and a second Cys residue at the cytoplasmic end of TM III (I169C(3.54

69 ontaining peptides, but the requirement of a Cys residue at the ligation junction can limit the utili

70 -specific drug conjugation at the engineered Cys residue at the position 239 of HC does not impact th

71 rast, mutants with Cys in the PAS only or no Cys residues at all exhibit red-shifted emission with sh

72 The introduction of Cys residues at homologous sites within either the beta

73 s S-palmitoylated on two adjacent C-terminal Cys residues at its cytoplasmic surface.

74 residue polypeptide chain of sfAFP contains Cys residues at positions 1, 13, 28, and 43 and was prep

75 ation site HX5CX17-18CX3-5H (HCCH), with His/Cys residues at positions 108, 114, 133, and 139 coordin

76 Likewise, the covalent modification of Cys residues at selected positions in the beta-ball-thum

77 Disulfide bonds formed between Cys residues at six positions in Ste2.

78 he formation of a disulfide bond between the Cys residues at the apocytochrome c heme-binding site (C

79 ) ions, which do not coordinate or influence Cys residues at the designed metal sites but are essenti

80 Pairs of Cys residues at the ends of two helices on the cytoplasm

81 d GPR109b identified a common structure (six Cys residues at the extracellular domains that potential

82 al for GPR81 function, and the conserved six Cys residues at the extracellular regions are necessary

83 hat BACE1 undergoes S-palmitoylation at four Cys residues at the junction of transmembrane and cytoso

84 The cysteine (Cys) residue at position 312 in the third transmembrane

85 an be derived from maurocalcine by replacing Cys residues by isosteric 2-aminobutyric acid residues a

86 ed by further lipid modification of adjacent Cys residues by one (N-ras) or two (H-ras) palmitoyls.

87 Substitution of both D3 Cys residues by Sec in the UAUA variant does not elimina

88 As predicted by this model, when Cys residues C141, C144, and C227 are mutated to alanine

89 of the heavy chain pairing with the terminal Cys residue (C214) in the light chain.

90 Ala substitutions at an additional conserved Cys residue (C291 in AtsB and C276 in anSMEcpe) afford p

91 ts overoxidation and inactivation, whereas a Cys residue can be permanently overoxidized to the sulfi

92 lation sites, Asp208 located between the two Cys residues, cannot undergo proper glycosylation, which

93 The reactivity of the Cys residues changed, sometimes dramatically, during the

94 Substitution of Tyr81 with a Cys residue, characteristic of established NIP boric aci

95 These three conserved Cys residues cluster with a previously unrecognized cons

96 s of each, and that the peptides contain six Cys residues, consistent with identities as alpha-defens

97 a Cu(4)S(6) species, and suggesting that D2 Cys residues contribute to the cluster.

98 d Hydrogenovibrio marinus, two supernumerary Cys residues coordinate the proximal [FeS] cluster in Ec

99 ed atomic force microscope tip to engineered Cys residues could be described by the worm-like chain m

100 the very N-terminus of Rrp44p contains three Cys residues (CR3 motif) that are conserved in many euka

101 dditional loop KSGKPLH, invariable cysteine (Cys) residues Cys-40 and Cys-168, and the conserved glut

102 ke proteins, Cr-TRP16 contains an additional Cys residue (Cys-15, at the N terminus), through which i

103 investigate its interaction with a conserved Cys residue (Cys-57) in SSP.

104 (Arg 86, Arg 91, Lys 100) and the anchoring Cys residues (Cys 85, Cys 101) within this motif were re

105 stant of the oxidation of PDI's redox-active Cys residues (Cys(53) and Cys(397)) by hydrogen peroxide

106 agment (residues 98-118), which contains two Cys residues (Cys-106 and Cys-112), was singly palmityla

107 mutations of either the last three alpha ECL Cys residues (Cys-14, -15, and -16) or Cys-7 within both

108 tion of ERp44C29S with SERT, which has three Cys residues (Cys-200, Cys-209, and Cys-109) on the seco

109 ever, VKOR contains evolutionarily conserved Cys residues (Cys-43 and Cys-51) that reside in a loop o

110 It uses a key Cys residue, Cys-13, to sense oxidative stress and to co

111 A conserved Cys residue, Cys-24, plays the major role of oxidative s

112 (i) Among its four Cys residues, Cys(52), Cys(83), and Cys(173) can be glut

113 Three cysteine (Cys) residues, Cys231, Cys329, and Cys330, in RPE65 have

114 al pocket residues (His64 and Val68) and two Cys residues (Cys55 and Cys120).

115 Mutation of two membrane-proximal Cys residues (Cys88/91) suppresses palmitoylation, and t

116 All four Cys residues (Cys9, 37, 40, and 46) in the N-terminal re

117 donor and acceptor probes at two engineered Cys residues designed to detect relay helix bending.

118 Replacing Ser239 with a Cys residue does not alter the exchange kinetics of the

119 Both proteins contain five conserved Cys residues, each required for turnover.

120 coli RNA polymerase based on the ability of Cys residues engineered into the TL and surrounding regi

121 of sources has identified a single conserved Cys residue essential for catalytic activity.

122 r in SepCysS by 3-fold, suggesting that this Cys residue facilitates the generation of the persulfide

123 oss-linking between selectively incorporated Cys residues finds two pairs of positions that provide f

124 ally, we demonstrated that a palmitoylatable Cys residue flanking the MYR site is crucial to localize

125 alpha-helical domain containing 14 conserved Cys residues followed by a glycosaminoglycan attachment

126 substitution of an evolutionarily conserved Cys residue for an Arg in the gene product.

127 Here, we show that the conserved Cys residues form a disulfide bond that inactivates the

128 MS was used to identify the specific Tyr and Cys residues forming radicals in the myoglobin system.

129 s: C8 evasins share a conserved set of eight Cys residues (four disulfide bonds), whereas C6 evasins

130 an intersubunit disulfide bond with a second Cys residue from the other subunit of the protein dimer.

131 luster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 fr

132 cause of the large increases in exposure for Cys residues from T13 to T51, we conclude that colicin B

133 MTSEA-biotin only interacts with the free Cys residues from the external phase of the plasma membr

134 We hypothesized that the deviation of Cys residues from the properties of a free amino acid is

135 exchange reactions between selected pairs of Cys residues from these proteins.

136 vide an overview of approaches used to study Cys residues, from methods for investigation of their ba

137 riants in which each of the two coordinating Cys residues has been individually mutated to Ala, using

138 f factors, palmitoylation of its cytoplasmic Cys residues has not been previously described.

139 However, the status of the Cys residues has remained unknown.

140 These Cys residues have been derivatized with a fluorescent pr

141 that are capable of identifying nitrosylated Cys residues have been developed.

142 The NIR FPs with both Cys residues have the narrowest blue-shifted spectra and

143 proteins that, although coordinated by four Cys residues, have different cluster environments.

144 conspicuously similar to that of a critical Cys residue in BtrN, another radical SAM dehydrogenase.

145 f S105 alleles encoding holins with a single Cys residue in different positions was developed and cha

146 ant located six residues after the conserved Cys residue in extracellular loop 2b (ECL2b) associated

147 It is unclear which Cys residue in Ibalpha, C484 or C485, forms the disulfid

148 unique chemistry of the invariant catalytic Cys residue in labeling the active site with biotinylate

149 ase of Escherichia coli (LacY) with a single-Cys residue in place of A122 (helix IV) transports galac

150 oinformatics analysis identified a conserved Cys residue in the ATP-binding site of the kinases repor

151 al gB proteins, the HCMV fusion factor has a Cys residue in the C-terminal region that is palmitoylat

152 compound covalently bound to a nucleophilic Cys residue in the catalytic site of the corresponding p

153 erredoxin-like fold that binds Cu(I) via two Cys residues in a M(10)X(11)C(12)X(13)X(14)C(15) motif l

154 Disulfide bonds between Cys residues in adjacent strands of parallel beta-sheets

155 d that only the first of the two active-site Cys residues in ATTRX5 is required for the response to v

156 BphP1-FP, iRFP670 and iRFP682 have Cys residues in both PAS and GAF domains, rather than in

157 These data suggest that Cys residues in D2 of hCCS are involved in the formation

158 found that mutation of the conserved His and Cys residues in HCCHp had little effect on secondary str

159 Mutation of one or both conserved Cys residues in HCCHp led to a decrease in Cys ligation,

160 eneration of filopodia is independent of the Cys residues in its redox active site, but dependent upo

161 ways via post-translational modifications of Cys residues in key regulatory proteins.

162 e role of a disulfide bond formed by vicinal Cys residues in maintaining calcium-bound TG2 in an inac

163 We present here that two Cys residues in MexR are redox-active.

164 Our analysis revealed that the ten Cys residues in murine Meteorin form five disulfide bond

165 We investigated the functional role of these Cys residues in Na(+) self-inhibition, an allosteric inh

166 f this study was to define the role of these Cys residues in palmitoylation, membrane association, an

167 igh-throughput and unbiased discovery of SNO-Cys residues in proteins from complex biological samples

168 d Tyr should undergo a similar reaction with Cys residues in proteins to give intramolecular or inter

169 This protocol describes the modification of Cys residues in proteins using glycomethanethiosulfonate

170 The three Cys residues in RPE65 were replaced by Alanine (Ala) wit

171 are other yet to be identified palmitylated Cys residues in RPE65.

172 y, we showed that all three highly conserved Cys residues in SepCysS (Cys(64), Cys(67), and Cys(272)

173 The CT-25 peptide contains the only two Cys residues in TE juxtaposed to a cluster of positively

174 N termini of 6K and TF, the four additional Cys residues in TF's unique C terminus, or all nine Cys

175 idues in TF's unique C terminus, or all nine Cys residues in TF.

176 rgely unaffected by modification of the same Cys residues in the absence of cross-link formation.

177 conclude that approximately half of the ECL Cys residues in the alpha and gamma ENaC subunits are re

178 previous work revealed that two cytoplasmic Cys residues in the beta subunit, betaCys-43 and betaCys

179 e two electrophiles, but BMCC alkylated four Cys residues in the C subunit that were not labeled by P

180 showed that ONOO(-) treatment modified both Cys residues in the CT-25 peptide to sulfonic acid deriv

181 n to tightly bind CO (K(d) = 50 nm) with the Cys residues in the CXXCH motif regulating affinity of t

182 by disulfide bonds formed between conserved Cys residues in the extracellular domain.

183 ylated, we now show that the two cytoplasmic Cys residues in the gamma subunit are palmitoylated.

184 la-123, and Leu-124) located between the two Cys residues in the HCCH motif disrupt binding of the zi

185 We modularly mutated the five Cys residues in the identical N termini of 6K and TF, th

186 structures and intersubunit cross-linking of Cys residues in the membrane.

187 short domain structure with an odd number of Cys residues in the metalloprotease domain.

188 This showed that all four conserved Cys residues in the N-terminal region are required for i

189 matic site-directed mutagenesis study of the Cys residues in the stem region shows that Cys(532) is i

190 dynamic post-translational modifications of Cys residues, in particular S-nitrosylation and S-oxidat

191 varies with the presence or absence of other Cys residues, indicating that the microenvironments of t

192 Introduction of Cys residues into the region between residues 28 and 34

193 s are extended to TM1 and TM2 of MotA, using Cys residues introduced in several positions in the segm

194 We observe that the catalytic Cys residue is covalently linked to the small-molecule i

195 Acylation of the KS active site Cys residue is followed by transfer to malonyl-ACP to yi

196 The solvent accessibility of the Cys residues is also determined by blockage of [14C]NEM

197 x control, acting through reactive cysteine (Cys) residues, is among the major mechanisms of redox re

198 sidue oxidizes more rapidly than a cysteine (Cys) residue, it has been previously thought that Sec-co

199 lity electron density map showing a modified Cys residue, likely connected to a long chain acyl group

200 Two conserved Cys residues located in the active site of PI4KIIIalpha

201 logs found in angiosperms contain two unique Cys residues located in the lumen.

202 eted by multiple Ox-PTM suggesting that this Cys residue may act as a redox sensor modulating ATP syn

203 In viruses with the N-terminal Cys residues mutated, TF is much less efficiently locali

204 between the catalytic Cys and one of the two Cys residues nearby, which is not observed in previously

205 -linkers covalently cross-link the catalytic Cys residue of the yeast HECT E3 ubiquitin ligase Rsp5 w

206 We show that six Cys residues of a precursor peptide are first cyclodehyd

207 csA via formation of disulfide bonds between Cys residues of adjacent subunits.

208 he active site of IscS and deliver it to the Cys residues of IscU, formed a disulfide bridge with Fdx

209 y S-nitros(yl)ation whereas the CXXC-derived Cys residues of MIF remained unaffected.

210 ite preferentially oxidizes the redox-active Cys residues of PDI to the corresponding sulfenic acids,

211 Here we show that the active site Cys residues of Prx2 form stable mixed disulfides with g

212 se basic residues are found between the loop Cys residues of several lentiviral fusion proteins, the

213 ivo resulted when two of the Zn-coordinating Cys residues of the ClpX ZBD were changed to Ser.

214 ified zinc ion and its coordination by three Cys residues of the IDC region of eukaryotic MutY organi

215 The two Cys residues of the motif are essential for the function

216 nd that reproducible saturation of available Cys residues of the proteins used as labeling standards

217 both E1 and E2 and places the two catalytic Cys residues of the two enzymes in close proximity.

218 Here we report that the conserved Cys residues of Tim22 form an intramolecular disulfide b

219 and in vitro while substitution of all three Cys residues of YodB affects induction of azoR1 transcri

220 so derivatives can also react with cysteine (Cys) residues of glutathione or proteins to form, respec

221 In this study we mutated the seven cysteine (Cys) residues of human PCFT to serine, creating Cys-less

222 Cysteine (Cys) residues often play critical roles in proteins; how

223 (-1)s(-1) consistent with a key role for the Cys residues on MPI as targets for haem protein-mediated

224 e natural collagen triple helices, cysteine (Cys) residues on neighboring strands are linked by disul

225 acid, disodium salt (IASD), to modify these Cys residues, one face of transmembrane domain 1 (TMD1)

226 Removing either the Cys residue or the two His residues lowers the Cu-peptid

227 in activity induced by cross-linkage of the Cys residue pairs was due to a decrease in the influxVma

228 ce of a terminal-free thiol group, such as a Cys residue, primarily due to better cellular uptake.

229 However, substitution of Ser596 with a Cys residue produced an active recombinant enzyme with c

230 helix 3, His264 (TM6), and Met292 (TM7)--to Cys residues produced definitive indications of proximit

231 Mutation of one of these Cys residues reduced the phenylarsine oxide sensitivity

232 but the exact role of the conserved His and Cys residues remains elusive.

233 the complex formed with the mutant with both Cys residues replaced was nearly identical with that of

234 king this disulfide, with one or both of the Cys residues replaced with Ser, were examined, and X-ray

235 a disulfide bond formed with an active-site Cys residue required for activity.

236 activity, whereas mutations of the conserved Cys residues resulted in a loss of the iron-sulfur clust

237 in Prx3, residues adjacent to the resolving Cys residue, resulted in a Prx2-like protein with increa

238 involving initial methylation of a conserved Cys residue (RlmN Cys(355)) by SAM.

239 O2 (1% O2), but their identity among the 100 Cys residues/RyR1 monomer is unknown.

240 n is typically restricted to one or very few Cys residue(s) in target proteins.

241 The chemical reactivities of engineered Cys residues scanned throughout the aspartate receptor H

242 n, pyrene maleimide was attached to pairs of Cys residues separated by ~5 A increments on helix 2 of

243 Disulfide cross-linking based on ectopic Cys residues showed that the contacts between Gag protei

244 Cys residues subject to pO2-coupled oxidation are distri

245 overage of RyR1 Cys residues) to identify 13 Cys residues subject to pO2-coupled S-oxidation in SR ve

246 Disulfide bonds also formed with Cys residues substituted for five different residues clu

247 of Ste2, a set of 73 different mutants with Cys residues substituted near the extracellular ends of

248 ohrR gene modified for N-terminal Cysteine (Cys) residue, suggesting that OhrR senses intracellular

249 The alpha6-helix ends in 2 Cys residues that are linked by an unusual vicinal disul

250 o significant increases in the reactivity of Cys residues that are located primarily at the same leve

251 formation of at most a few final pairings of Cys residues that may be separated by significant interv

252 ons in a previously unseen manner via mainly Cys residues that point into the core of the bundle.

253 etrameric proteins possess a large number of Cys residues that point into the cores of their four-hel

254 Knowledge of the specific protein Cys residues that undergo NO addition in different biolo

255 agnetic nitroxide spin label was attached to Cys residues that were placed into the protein at positi

256 investigated the arrangement of the His and Cys residues, the role of the spacing between them, and

257 In a cyclization process, Cys residues then attack the dehydrated residues to gene

258 rgI engineered with an accessible N-terminal Cys residue to 20kDa PEG-maleimide (Co-hArgI-C(PEG-20K))

259 Mutation of the palmitoylated Cys residue to Ala or inhibition of protein palmitoylati

260 partners and by inserting an extra, unpaired Cys residue to create an opportunity for intermolecular

261 through the hyperoxidation of an active site Cys residue to Cys sulphinic acid.

262 They use a conserved Cys residue to reduce peroxide substrates.

263 mical ligation followed by conversion of the Cys residues to Ala by Raney nickel desulfurization.

264 substrate influx as was accessibility of the Cys residues to biotinylation.

265 ively, followed by the conjugate addition of Cys residues to the Dha and Dhb residues to generate the

266 by examining the accessibility of introduced Cys residues to the sulfhydryl reagent MTSET.

267 Phase-I conversion of six Cys residues to thiazoles (TclIJN) is followed by C-term

268 spectrometry (yielding 93% coverage of RyR1 Cys residues) to identify 13 Cys residues subject to pO2

269 nce the widespread occurrence of the Trp/Cys-Cys residues triads in proteins make our procedure very

270 olecular disulfide bonds involving all three Cys residues, two in Rz and one in Rz1.

271 Quantification of the redox state of 1098 Cys residues using OxICAT revealed that 381 Cys residues

272 ation inhibitory factor contains 3 cysteine (Cys) residues; using recombinant wtMIF and site-specific

273 respectively, that are attached to specific Cys residues via thioether linkages.

274 In addition, clustering of Cys with another Cys residue was associated with high conservation, where

275 to process EbpB and its predicted catalytic Cys residue was required for efficient cell wall anchori

276 A conserved Cys residue was shown to be necessary for redox regulati

277 proteins encoded by A. oris possess multiple Cys residues, we propose that MdbA and VKOR constitute a

278 Pairs of Cys residues were assessed for spontaneous formation of

279 sonance assignments for the isotope-enriched Cys residues were determined with 2D versions of standar

280 ellular region, two CXC motifs and two other Cys residues were found to be involved in disulfide brid

281 These surface Cys residues were further modified by the sulfhydryl-spe

282 n alpha and gamma ECLs and selected beta ECL Cys residues were individually mutated to alanine or ser

283 Cys residues were introduced at 12 positions in hSERT to

284 Cys residues were introduced at various positions in Fli

285 (6.9 +/- 0.2) x 10(4) m(-1) s(-1)), and both Cys residues were kinetically indistinguishable.

286 pO2-sensitive Cys residues were largely non-overlapping with those ide

287 S-glutathionylated nor sulfenamide-modified Cys residues were observed.

288 Linear analogs of TP-I where the four Cys residues were replaced by aromatic and aliphatic res

289 belling, indicating either that the relevant Cys residues were sequestered by an unknown protein or t

290 complexes in which all endogenous cysteine (Cys) residues were eliminated by site-directed mutagenes

291 ethyltransferases (DNMTs) through the active Cys residue, which provides a new tool to covalently tra

292 y S-oxidation or S-nitrosylation of separate Cys residues, which are allosterically linked.

293 e, providing an alternative way to transform Cys residues, which were artificially inserted into a pe

294 Covalent modification of the Cys residues with a structurally diverse set of hydropho

295 ouble Cys mutant M3 receptors containing one Cys residue within the sequence K484(6.29) to S493(6.38)

296 Moreover, mutations of a single Cys residue within the TMD of Cosmc prevent formation of

297 TuSp1, demonstrating that TuSp1 contains two Cys residues within a nonrepetitive N-terminal region.

298 All 16 Cys residues within alpha and gamma ECLs and selected be

299 P79/150 required its depalmitoylation on two Cys residues within the N-terminal targeting domain.

300 sidues 81 to 155, and to our surprise, the 5 Cys residues within UL16(1-155) are not required, even t