ライフサイエンスコーパス: Cys

1 Cys accessibility and quantitative intact mass spectrome

2 Cys-SSH and GSSH are produced in the brain of wild-type

3 Cys-SSH and its glutathione (GSH) counterpart (GSSH) hav

4 rn but instead required two conserved Trp(1)-Cys(2) residues at the N terminus.

5 A FSH motif, conserved in 1-Cys Prxs, precedes the active site PxxxTxxCp signature a

6 Yeast Prx1 is a mitochondrial 1-Cys peroxiredoxin that catalyzes the reduction of endoge

7 ttle is known about the catalytic cycle of 1-Cys Prxs.

8 xxCp signature and might contribute to the 1-Cys Prx reaction cycle.

9 vel sites of adduction were found; alpha(104)Cys and beta(112)Cys.

10 Quantification of the redox state of 1098 Cys residues using OxICAT revealed that 381 Cys residues

11 ction were found; alpha(104)Cys and beta(112)Cys.

12 Furthermore, steric obstruction of Cys-119/Cys-162 by NO2-OA pretreatment in Langendorff-perfused m

13 a activation and covalently modified Cys-119/Cys-162, probably obstructing MKK3 access.

14 s loop and the lack of the conserved Cys-139-Cys-206 disulfide bond.

15 tomes of the spider Cupiennius salei have 15 Cys-loop receptor subunits and an acetylcholine-binding

16 In addition, an HIF-1alpha Cys(520) serine mutant is resistant to 2-AAPA-induced HI

17 in APC-mutation-positive colorectal cancer.2-Cys peroxiredoxin (Prx) enzymes are highly expressed in

18 inetics for the oxidation of the cytosolic 2-Cys Prx1 and Prx2 revealed that urate hydroperoxide oxid

19 ype Trxs, was also suppressed by decreased 2-Cys Prxs contents, as the ntrc-trxf1f2-Delta2cp mutant p

20 Mammalian 2-Cys peroxiredoxin (Prx) enzymes are overexpressed in mos

21 functional gain that allows mitochondrial 2-Cys peroxiredoxins to act as molecular chaperones when f

22 Thus, the NTRC, 2-Cys Prxs, and Fd-FTR-Trxs redox systems may act concerte

23 chanism to stabilize the decameric form of 2-Cys peroxiredoxins in Leishmania mitochondria.

24 Here we show that decreased levels of 2-Cys Prxs suppress the phenotype of the Arabidopsis thali

25 systems are linked by the redox balance of 2-Cys Prxs, which is crucial for chloroplast function.

26 The excess of oxidized 2-Cys Prxs in NTRC-deficient plants drains reducing power

27 NTRC efficiently reduces 2-Cys peroxiredoxins (Prxs), thus having antioxidant funct

28 talytic cycle has been derived for typical 2-Cys Prxs, however, little is known about the catalytic c

29 f an intramolecular disulfide bond (Cys(318)-Cys(326)), known to act as a redox switch that precludes

30 ted in a higher basal oxidation level of 338 Cys residues (41.1%).

31 in celiac disease and establish the Cys(370)-Cys(371) disulfide bond of TG2 as one of clearest exampl

32 Cys residues using OxICAT revealed that 381 Cys residues (33.6%) showed >10% increased oxidations un

33 isulfides (i.e. Cys(53)-Cys(56) and Cys(397)-Cys(400)).

34 In contrast, the Cys(4)-Cys(21) bridge is predicted to form together with either

35 ve sites to produce disulfides (i.e. Cys(53)-Cys(56) and Cys(397)-Cys(400)).

36 Moreover, we found that (55)Cys helps to determine the influence of beta2 on Nav1.2

37 PA-1 was greatly enhanced while adding 2.5nM Cys.

38 We identified a flexible loop formed by (72)Cys and (75)Cys, a unique feature among the four beta-su

39 ed a flexible loop formed by (72)Cys and (75)Cys, a unique feature among the four beta-subunit isofor

40 to previously reported adduction of beta(93)Cys of human Hb, two novel sites of adduction were found

41 We identified the conserved cysteine 97 (Cys-97) to be the only reactive thiol in human MCU that

42 In addition to Trp(233*+), a Cys(222)-derived radical was identified by electron para

43 a long loop between helices I and II, and a Cys residue as a quencher for the acceptor.

44 he intramolecular cyclodehydration between a Cys, Ser, or Thr side chain and the backbone carbonyl ca

45 arbon-nitrogen hydrolase domain containing a Cys-Glu-Lys catalytic triad.

46 were found to enhance GLP-1R signaling in a Cys-347-dependent manner.

47 TbtI C-methylates a Cys-derived thiazole during posttranslational maturation

48 irectly examine this concept, we utilized a "Cys-lite" neuronal NOS flavoprotein domain and substitut

49 (OMIM: 613319, 611307); the same amino acid (Cys) at the position 356 is mutated in GDD.

50 stant of the oxidation of PDI's redox-active Cys residues (Cys(53) and Cys(397)) by hydrogen peroxide

51 ite preferentially oxidizes the redox-active Cys residues of PDI to the corresponding sulfenic acids,

52 ytochrome c and the identity of their active Cys residues are unknown.

53 N termini of 6K and TF, the four additional Cys residues in TF's unique C terminus, or all nine Cys

54 tudies revealed that ET between Trp(233) and Cys(222) is possible and likely to participate in the ca

55 juxtamembrane cysteine residues, Cys-264 and Cys-265.

56 ntiation factor 15), GAL-3 (galectin-3), and Cys-C (cystatin-C) were assessed before TAVR and in 100

57 oplasmic domains, with residues Ala(463) and Cys(466) buried within the trimer interface of the sulfu

58 PDI's redox-active Cys residues (Cys(53) and Cys(397)) by hydrogen peroxide (k = 17.3 +/- 1.3 m(-1) s

59 produce disulfides (i.e. Cys(53)-Cys(56) and Cys(397)-Cys(400)).

60 ratios for (111)In-labeled Cys(2)-ADAPT6 and Cys(59)-ADAPT6 did not differ significantly (250-280), b

61 Both Cys(2)-ADAPT6 and Cys(59)-ADAPT6 were internalized slowly by HER2-expressi

62 Two constructs, Cys(2)-ADAPT6 and Cys(59)-ADAPT6, having the (HE)3DANS sequence at the N t

63 We further show that Cys-45 of CcmH and Cys-34 of apocytochrome c are most likely to form this m

64 n had higher N-glycosylation efficiency, and Cys, in particular, compensated for the negative effect

65 in a 6-coordinate complex with axial His and Cys ligands, the latter provided by a heme-regulatory mo

66 s(II)-Cys(III)), or bead (Cys(I)-Cys(II) and Cys(III)-Cys(IV)).

67 onnectivities: globular (Cys(I)-Cys(III) and Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys(IV) and Cys(II)-Cys

68 Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys(IV) and Cys(II)-Cys(III)), or bead (Cys(I)-Cys(II) and Cys(III)-

69 ive inhibition of a large number of Ser- and Cys-containing enzymes participating in important physio

70 ifferent polarity and length (i.e. Ala, Arg, Cys, His, Glu, and Leu) on transporter stability and fun

71 Single amino acid changes around Cys-739 in FL-NCX1 and deletions on the N-terminal side

72 s phylogenetically conserved zinc-associated Cys-X-X-Cys motif near the catalytic domain of the prote

73 n-covalently and to form covalent adducts at Cys-34, suggesting potential modes for systemic distribu

74 ngs, we observed that disulfide formation at Cys(43) does not directly activate PKGIalpha in vitro or

75 In conclusion, disulfide formation at Cys(43) does not directly activate PKGIalpha, and the C4

76 ally dependent on palmitoylation of Glut4 at Cys-223.

77 In addition, beta2AR S-palmitoylated at Cys-265 are selectively preserved under a sustained adre

78 n phosphatase-1 (PP1c) via persulfidation at Cys-127.

79 The Au/Cys/FcPAMAM/anti-PSA immunosensor showed excellent perfo

80 s(I)-Cys(IV) and Cys(II)-Cys(III)), or bead (Cys(I)-Cys(II) and Cys(III)-Cys(IV)).

81 work has identified a disulfide bond between Cys-45 residues within the homodimer interface of Rgg2 f

82 rmation of an intramolecular disulfide bond (Cys(318)-Cys(326)), known to act as a redox switch that

83 Both Cys(2)-ADAPT6 and Cys(59)-ADAPT6 were internalized slowl

84 (6.9 +/- 0.2) x 10(4) m(-1) s(-1)), and both Cys residues were kinetically indistinguishable.

85 For this we introduced a buried Cys at the identical location in each FNIII domain and m

86 ast majority of pentameric receptors (called Cys-loop receptors in eukaryotes) present physiologicall

87 ione, while those with longer sulfur chains, Cys-SSnH and GSSnH, were produced in the presence of low

88 ields of H2S were obtained from the combined Cys/GSH and copper treatments in white wine.

89 ues and contain ten evolutionarily conserved Cys residues across a wide variety of species.

90 Interestingly, replacement of the conserved Cys at the metal binding pocket leads to a large reducti

91 autolysis loop and the lack of the conserved Cys-139-Cys-206 disulfide bond.

92 Two constructs, Cys(2)-ADAPT6 and Cys(59)-ADAPT6, having the (HE)3DANS s

93 e by modifying Au electrode with cysteamine (Cys) and immobilization of ferrocene cored polyamidiamin

94 Cysteine (Cys) and glutathione (GSH) were associated with small in

95 on of the organic complexing agent cysteine (Cys) alongside Ag only marginally moderated toxicity, im

96 Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play cru

97 Biothiols, such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play a

98 s changing in abundance, including cysteine (Cys)-rich receptor-like kinases (CRKs) that are up-regul

99 es through covalent binding at its cysteine (Cys) thiol group, followed by stepwise catalyzed degrada

100 ectively over other biothiols like cysteine (Cys) and homo-cysteine (H-cys) with a DL of 43nM, even i

101 ohrR gene modified for N-terminal Cysteine (Cys) residue, suggesting that OhrR senses intracellular

102 In this study, we used cysteine (Cys) mutants to test differential palmitoylation of the

103 mong the synthesized analogue, Ac-Arg-Ala-[d-Cys-Arg-Phe-His-Pen]-COOH (19), displayed subnanomolar a

104 ve, and plasma stable peptide, Ac-Arg-Ala-[d-Cys-Arg-Phe-Phe-Cys]-COOH (3).

105 The observed binding modes of Hcy and D-Cys clarify why they are not substrates, and the binding

106 f sstr antagonists, the analog JR11 (Cpa-c[d-Cys-Aph(Hor)-d-Aph(Cbm)-Lys-Thr-Cys]-d-Tyr-NH2), an anta

107 ioned characteristics and the ability of DCM-Cys to provide selective, nanomolar-level in vitro cyste

108 Herein is described a new probe, DCM-Cys, that preferentially reacts with cysteine to form a

109 40 nm) are red-shifted from those of the DCM-Cys probe (lambdaabs(probe) = 440 nm, lambdaemis(probe)

110 tributed to the molecular designs of the DCM-Cys probe and DCM reporter.

111 hat encode PORB mutant proteins with defined Cys-->Ala exchanges.

112 es Cys and l-Cys desulfhydrase that degrades Cys to H2S, NH3, and pyruvate.

113 e PA-1 and its ability to selectively detect Cys in living cells.

114 multiple binding site probes to distinguish Cys, Hcy, and GSH is highlighted as a creative new direc

115 er significantly (250-280), but (111)In-DOTA-Cys(59)-ADAPT6 provided significantly higher tumor-to-lu

116 the active sites to produce disulfides (i.e. Cys(53)-Cys(56) and Cys(397)-Cys(400)).

117 es: a high-affinity Site 1 required for E6AP Cys(820) approximately ubiquitin thioester formation and

118 s: C8 evasins share a conserved set of eight Cys residues (four disulfide bonds), whereas C6 evasins

119 ural and functional model for the eukaryotic Cys-loop receptor superfamily.

120 l death, which required intact extracellular Cys residues and a conserved kinase active site.

121 CRKs possess extracellular Cys-rich domains and constitute a gene family consisting

122 thic alpha-helix whose properties facilitate Cys-739 palmitoylation.

123 We modularly mutated the five Cys residues in the identical N termini of 6K and TF, th

124 The limits of detection (LOD) for Cys, Hcy and GSH were 0.156, 0.185, and 1.838 muM, respe

125 uble and a biologically acceptable probe for Cys-SeH.

126 lable uricosuric agents, the requirement for Cys-32 is unique to verinurad.

127 ndings reveal a distinct functional role for Cys-97 in ROS sensing and regulation of MCU activity.

128 the sulfate reductive pathway that generates Cys and l-Cys desulfhydrase that degrades Cys to H2S, NH

129 possible disulfide connectivities: globular (Cys(I)-Cys(III) and Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys

130 re characterized by divergent levels of GSH, Cys, and Hcy.

131 the first time that histidine-cysteine (His-Cys) and histidine-lysine (His-Lys) in addition to histi

132 ellular Golgi apparatus-specific Asp-His-His-Cys (DHHC) zinc finger protein.

133 HC3), a Golgi apparatus-specific Asp-His-His-Cys (DHHC) zinc finger protein; (ii) a GODZ dominant-neg

134 milar tumor-to-organ ratios, but (125)I-HPEM-Cys(59)-ADAPT6 had significantly higher tumor uptake and

135 dionuclide therapy using (131)I-labeled HPEM-Cys(59)-ADAPT6.

136 s(IV) and Cys(II)-Cys(III)), or bead (Cys(I)-Cys(II) and Cys(III)-Cys(IV)).

137 e disulfide connectivities: globular (Cys(I)-Cys(III) and Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys(IV) an

138 ys(III) and Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys(IV) and Cys(II)-Cys(III)), or bead (Cys(I)-Cys(II) a

139 Cys(IV)), ribbon (Cys(I)-Cys(IV) and Cys(II)-Cys(III)), or bead (Cys(I)-Cys(II) and Cys(III)-Cys(IV))

140 ities: globular (Cys(I)-Cys(III) and Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys(IV) and Cys(II)-Cys(III)),

141 (III)), or bead (Cys(I)-Cys(II) and Cys(III)-Cys(IV)).

142 epresents a novel allosteric binding site in Cys-loop receptors.

143 ant C53A DJ-1 shows potentiated H2O2-induced Cys-106 hyperoxidation.

144 Glycine receptors (GlyR) are inhibitory Cys-loop ion channels that contribute to the control of

145 ial adhesins have revealed an intramolecular Cys-Gln thioester bond that can react with surface-assoc

146 ffect of covalent modification of introduced Cys at the domain-domain interfaces.

147 luster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 fr

148 ontrast, an Abc3 mutant in which an inverted Cys-Pro motif had been replaced with Ala residues fails

149 We investigated Cys-loop gene expression in muscle tissue by qPCR and lo

150 p probe of TPETH-2(CFTERD3) (where CFTERD is Cys-Phe-Thr-Glu-Arg-Asp) was developed for chymase detec

151 e reductive pathway that generates Cys and l-Cys desulfhydrase that degrades Cys to H2S, NH3, and pyr

152 ns of elevated ROS, endogenous L-cysteine (L-Cys) production is insufficient for GSH synthesis.

153 h halophytes are enzymes that also degrade l-Cys to H2S.

154 s sustained depletion of the extracellular L-Cys and CSSC pool in mice and non-human primates.

155 level in Sarcocornia is the result of high l-Cys degradation rate by OAS-TLs, whereas the greater org

156 the result of higher APR activity and low l-Cys degradation rate, resulting in higher net Cys biosyn

157 This necessitates uptake of L-Cys that is predominantly in its disulfide form, L-cysti

158 (OAS-TL; EC 2.5.1.47) is the formation of l-Cys, but our study shows that the OAS-TL A and OAS-TL B

159 The ds-DNA/p(L-Cys)/Fe3O4 NPs-GO/CPE exhibited an increase in peak curr

160 3O4 nanoparticles-graphene oxide (ds-DNA/p(L-Cys)/Fe3O4 NPs-GO/CPE) for sensitive detection of adenin

161 hat enzyme-mediated depletion of the serum L-Cys and CSSC pool suppresses the growth of multiple tumo

162 Tumor-to-blood ratios for (111)In-labeled Cys(2)-ADAPT6 and Cys(59)-ADAPT6 did not differ signific

163 induces a conformational change that limits Cys-106 forming heterodisulfide protein complexes or fro

164 FPheK reacted with adjacent Lys, Cys, and Tyr residues in thioredoxin in high yields.

165 i opens a new avenue for producing mammalian Cys-loop receptors to facilitate structure-based rationa

166 Both oxidation and mutation of MCU Cys-97 exhibited persistent MCU channel activity with hi

167 ommendations, having e.g. 4.8% Lys, 2.7% Met+Cys, and 7.7% Phe+Tyr.

168 ociated with skeletal muscle glutathione/Met/Cys metabolism (2-hydroxybutanoic acid, oxoproline, Gly,

169 p38alpha activation and covalently modified Cys-119/Cys-162, probably obstructing MKK3 access.

170 ss, which overlapped with 40 S-mycothiolated Cys-peptides.

171 modifies specific cysteine residues (namely, Cys-257, -273, -288, -434, -489, and -613) within Keap1,

172 e) capped lanthanum hydroxide nanoparticles [Cys-La(OH)3 NPs] towards the fabrication of efficient im

173 ys degradation rate, resulting in higher net Cys biosynthesis.

174 idues in TF's unique C terminus, or all nine Cys residues in TF.

175 rast, mutants with Cys in the PAS only or no Cys residues at all exhibit red-shifted emission with sh

176 levels and stabilization of an artificial Nt-Cys substrate and ERFVII function in response to environ

177 ociated metabolism, such as lower amounts of Cys and glutathione, as well as a differential compositi

178 be attributed to the nucleophilic attack of Cys to the alpha,beta-unsaturated ketone resulting in sw

179 g experiments that exploited the capacity of Cys-306 to form intermonomeric disulfide bridges in the

180 The characterization of Cys-La(OH)3 NPs was carried out by different techniques

181 uccessfully applied for the determination of Cys in blood serum samples.

182 Likewise, the covalent modification of Cys residues at selected positions in the beta-ball-thum

183 ways via post-translational modifications of Cys residues in key regulatory proteins.

184 Mutagenesis of Cys-298 confirmed its role in dimerization.

185 Mutation of Cys that blocked ENaC palmitoylation also reduced channe

186 Mutation of Cys-102 that strongly affected slow channel closing slow

187 Specific nitrosylation of Cys-226 decreases NAB1 activity and could be demonstrate

188 Furthermore, steric obstruction of Cys-119/Cys-162 by NO2-OA pretreatment in Langendorff-pe

189 a cGMP-independent fashion via oxidation of Cys(43), resulting in disulfide formation within the PKG

190 exchange reactions between selected pairs of Cys residues from these proteins.

191 Basal S-palmitoylation of Cys-265 is negligible, but agonist-induced beta2AR activ

192 eactions of the two-step indirect pathway of Cys-tRNA(Cys) synthesis (tRNA-dependent cysteine biosynt

193 However, the production of Cys-SSH and GSSH is not well understood.

194 meric cyclic hexapeptide with replacement of Cys by Ala.

195 Furthermore, replacement of Cys-575 in the IgM tail piece of multimers resulted in m

196 omatic amino acids on the C-terminal side of Cys-739 abolished YFP-NCX1 palmitoylation.

197 NCX1 and deletions on the N-terminal side of Cys-739 in YFP-NCX1 did not affect NCX1 palmitoylation,

198 which catalyze the addition of the thiol of Cys to dehydrated Ser residues during the biosynthesis o

199 izes HIF-1alpha by increasing GSH adducts on Cys(520) promoting in vivo HIF-1alpha stabilization, VEG

200 ins a Cu atom coordinated by two His and one Cys in a trigonal plane, with an axial H2O at 2.25 A.

201 We predicted that Cys-119 or Cys-162 of p38alpha, close to the known MKK3 docking dom

202 ple residues of hERG1 were mutated to Ala or Cys and the resulting mutant channels were heterologousl

203 annel activity, and here we show that Ala or Cys substitutions of the functionally equivalent residue

204 cipitation assay, substitution of the His or Cys heme ligands in Rev-erbbeta was accompanied by a sig

205 tion, blockade, and regulation of pentameric Cys-loop ion channels at the atomic level.

206 ly active cysteine residues, the peroxidatic Cys (CP) and, if present, the resolving Cys (CR).

207 Cysteine-persulfide (Cys-SSH) is a cysteine whose sulfhydryl group is covalen

208 iscovered a four-residue pi-clamp motif (Phe-Cys-Pro-Phe) for regio- and chemoselective arylation of

209 table peptide, Ac-Arg-Ala-[d-Cys-Arg-Phe-Phe-Cys]-COOH (3).

210 factors (ERF-VII) that, together with plant Cys oxidases, act as an oxygen-sensing mechanism.

211 SDS-PAGE confirms assembly of the predicted Cys(820)-linked (125)I-polyubiquitin thioester intermedi

212 n of N-acetylcysteine, a commonly prescribed Cys supplement drug to Cys by aminoacylase-1 (ACY-1), an

213 In a cyclization process, Cys residues then attack the dehydrated residues to gene

214 topyruvate sulfurtransferase (3MST) produces Cys-SSH and GSSH together with the potential signaling m

215 trate that PCO dioxygenase activity produces Cys-sulfinic acid at the N terminus of an ERF-VII peptid

216 tylcholine receptor, which is a prototypical Cys-loop receptor.

217 bound to a protein containing four proximal Cys thiols-a tetracysteine (Cys4) motif.

218 Different side chains replacing Cys(93) profoundly reduced RD3 affinity for the cyclase,

219 alent interaction with a unique JAK3 residue Cys-909.

220 tural modeling predicted a cysteine residue (Cys-298) in position to form a disulfide bridge between

221 uric acid transport requires URAT1 residues Cys-32, Ser-35, Phe-365 and Ile-481.

222 xidation of PDI's redox-active Cys residues (Cys(53) and Cys(397)) by hydrogen peroxide (k = 17.3 +/-

223 tion on two juxtamembrane cysteine residues, Cys-264 and Cys-265.

224 atic Cys (CP) and, if present, the resolving Cys (CR).

225 apocytochrome c, as well as their respective Cys mutant variants, we determined the rates of thiol-di

226 ys(I)-Cys(III) and Cys(II)-Cys(IV)), ribbon (Cys(I)-Cys(IV) and Cys(II)-Cys(III)), or bead (Cys(I)-Cy

227 These can form [Cu4 (S-Cys)4 ] intermediates leading to [Cu4 (S-Cys)5 ](-) , [C

228 s)5 ](-) , [Cu4 (S-Cys)6 ](2-) , and [Cu4 (S-Cys)5 (O-Asn)](-) clusters.

229 (S-Cys)4 ] intermediates leading to [Cu4 (S-Cys)5 ](-) , [Cu4 (S-Cys)6 ](2-) , and [Cu4 (S-Cys)5 (O-

230 ates leading to [Cu4 (S-Cys)5 ](-) , [Cu4 (S-Cys)6 ](2-) , and [Cu4 (S-Cys)5 (O-Asn)](-) clusters.

231 peptides containing nucleophilic sidechains (Cys, His, and Lys) and selected proteins (bovine and hum

232 (called RING2) that contains an active site Cys required for the formation of an obligatory E3 Ub in

233 a disulfide bond formed with an active-site Cys residue required for activity.

234 onal NOS flavoprotein domain and substituted Cys for two residues (Glu-816 and Arg-1229) forming a sa

235 Since the ten Cys residues are highly conserved in Meteorin and Cometi

236 Our analysis revealed that the ten Cys residues in murine Meteorin form five disulfide bond

237 In the C-terminal Cys mutant, TF protein levels increase both in the cell

238 arbonyl-protecting group from the N-terminal Cys in a similar efficiency.

239 In viruses with the N-terminal Cys residues mutated, TF is much less efficiently locali

240 e specific for substrates bearing N-terminal Cys residues.

241 is provides molecular evidence of N-terminal Cys-sulfinic acid formation and arginylation by N-end ru

242 nd that, after addition of a single-terminal Cys residue, a CdtB homologue from cytolethal distending

243 sitive allosteric modulator, determined that Cys-347 in the GLP-1R is required for positive allosteri

244 We predicted that Cys-119 or Cys-162 of p38alpha, close to the known MKK3

245 We further show that Cys-45 of CcmH and Cys-34 of apocytochrome c are most li

246 tes of COS cells expressing 3MST showed that Cys-SSH and GSSH were produced in the presence of physio

247 Mutational analysis suggested that Cys(609) in GC1 is involved in the Trx1-GC1 association

248 The Cys ligands in the apo-form are preorganized for binding

249 of conserved structural domains such as the Cys-loop (L170R) and M2-M3 loop (A305V) that form the GA

250 he formation of a disulfide bond between the Cys residues at the apocytochrome c heme-binding site (C

251 s during flooding) is directly sensed by the Cys-Arg/N-end rule pathway of ubiquitin-mediated proteol

252 In contrast, the Cys(4)-Cys(21) bridge is predicted to form together with

253 e of TRX in celiac disease and establish the Cys(370)-Cys(371) disulfide bond of TG2 as one of cleare

254 50) serves as a general base to generate the Cys(820) thiolate within the low dielectric binding inte

255 Peptide studies identified the Cys thiolate as the most reactive nucleophile for these

256 he(170) of the conserved FPF sequence of the Cys loop, and that these interactions affect potentiatin

257 ed that LanCL1 catalyzes the addition of the Cys of glutathione to protein- or peptide-bound dehydroa

258 However, the status of the Cys residues has remained unknown.

259 y ubiquitin thioester bond within 6 A of the Cys(820) nucleophile.

260 on between the open and closed states of the Cys-loop receptors.

261 ne the overall structure and topology of the Cys-loop receptors.

262 Cu(II) coordination enables formation of the Cys-Tyr cross-link.

263 O2 Using purified enzymes, we found that the Cys(43) oxidation had no effect on basal kinase activity

264 sense multiple abiotic stresses through the Cys-Arg/N-end rule pathway either directly (via oxygen s

265 Thus, the Cys loop acts as a key control element in the allosteric

266 09)) and Phe(167), a residue adjacent to the Cys loop FPF motif, also affect dFBr potentiating effica

267 he binding sites for these modulators to the Cys loop, a region that is critical for channel gating i

268 Oxygen sensing via the Cys-Arg/N-end rule in higher eukaryotes is linked throug

269 es for betaEST and dFBr communicate with the Cys loop, through interactions between the last residue

270 channel gating through interactions with the Cys loop.

271 These Cys-La(OH)3 NPs were electrophoretically deposited onto

272 JR11 = Cpa-c(dCys-Aph(Hor)-dAph(Cbm)-Lys-Thr-Cys)-dTyr-NH2)) for PET imaging.

273 JR11 = Cpa-c(dCys-Aph(Hor)-dAph(Cbm)-Lys-Thr-Cys)-dTyr-NH2)), a novel radiolabeled sst receptor antag

274 DOTA-[Cpa-c(DCys-Aph(Hor)-DAph(Cbm)-Lys-Thr-Cys)-DTyr-NH2]) labeled with (177)Lu, (90)Y, and (111)In

275 R11 (Cpa-c[d-Cys-Aph(Hor)-d-Aph(Cbm)-Lys-Thr-Cys]-d-Tyr-NH2), an antagonist with selectivity for sstr

276 The three Cys mutants have minor defects in cell culture growth bu

277 intracellular organic hydroperoxides through Cys residue.

278 tin chains from the proximal end attached to Cys(820) before stochastic en bloc transfer to the targe

279 estingly, thiosulfate is covalently bound to Cys(330) on heme 3.

280 hment of linear tetrapyrrole chromophores to Cys-155 of phycobiliprotein beta-subunits, suggesting th

281 a commonly prescribed Cys supplement drug to Cys by aminoacylase-1 (ACY-1), an important and endogeno

282 Covalent bond formation to Cys-154 was confirmed by incubation of the inhibitors wi

283 rminal propeptide being covalently linked to Cys-319 and thereby hindering homodimerization.

284 e (SepCysS), which catalyzes the Sep-tRNA to Cys-tRNA conversion in methanogens, also possess a [3Fe-

285 e, providing an alternative way to transform Cys residues, which were artificially inserted into a pe

286 f SepCysE each bind SepRS, SepCysS, and tRNA(Cys), respectively, which mediates the dynamic architect

287 of the two-step indirect pathway of Cys-tRNA(Cys) synthesis (tRNA-dependent cysteine biosynthesis) to

288 ables a global long-range channeling of tRNA(Cys) between SepRS and SepCysS distant active sites.

289 a mismatching O-phosphoserine (Sep) to tRNA(Cys) followed by the conversion of tRNA-bounded Sep into

290 logs as well as phosphoseryl-tRNA (Sep-tRNA):Cys-tRNA synthase (SepCysS), which catalyzes the Sep-tRN

291 f tRNA-bounded Sep into cysteine by Sep-tRNA:Cys-tRNA synthase (SepCysS).

292 Using the technique of Trp-Cys contact quenching, we investigate the effects of var

293 P79/150 required its depalmitoylation on two Cys residues within the N-terminal targeting domain.

294 e dimer formation, suggesting that these two Cys residues act as vicinal thiols, consistent with C119

295 peroxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases.

296 knowledge, the peptides Gly-Pro-Ala-Val, Val-Cys, and Phe-Phe have not been previously identified to

297 ites and six intrachain cystine bridges with Cys-158 of the very flexible N-terminal propeptide being

298 In contrast, mutants with Cys in the PAS only or no Cys residues at all exhibit re

299 n1, whereas substitution of talin2 S339 with Cys diminished that of talin2.

300 enetically conserved zinc-associated Cys-X-X-Cys motif near the catalytic domain of the protein, decr