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1 D. pseudoobscura and D. affinis were used for the betwee
2 for the five loci show that gene flow among D. pseudoobscura populations is sufficient to homogenize
5 h two other loci, in both D. p. bogotana and D. pseudoobscura, strongly suggest this reduction is due
7 d and not shared between D. melanogaster and D. pseudoobscura show that there are separate classes un
8 am protein is present in D. melanogaster and D. pseudoobscura testes, cognate proteins for the gene1/
10 osomal rearrangements between D. miranda and D. pseudoobscura are far higher than those found before
11 atural populations of Drosophila miranda and D. pseudoobscura, together with their sequence divergenc
12 bscura and close relatives D. persimilis and D. pseudoobscura bogotana has been studied using compara
18 All species of the Drosophila subgenus and D. pseudoobscura of the Sophophora subgenus completely l
20 the genetic basis of the difference between D. pseudoobscura and D. persimilis in two courtship song
21 e fraction of amino acid differences between D. pseudoobscura and D. affinis appear to have been fixe
22 lasmic incompatibility system exists between D. pseudoobscura strains BogER and AH162; and, that BogE
23 gned coding and non-coding sequences between D. pseudoobscura and D. miranda, and investigated their
25 lent sites is, on average, very weak in both D. pseudoobscura and D. simulans (magnitude of NS approx
27 sophila melanogaster genes, as well as fewer D. pseudoobscura and D. virilis genes, was examined from
28 mated times since speciation are one mya for D. pseudoobscura and D. persimilis and 2 mya since the f
30 ed the knot wing cis-regulatory element from D. pseudoobscura, which contains a cluster of UBX-bindin
32 ionally, we utilized recombination maps from D. pseudoobscura and D. miranda to explore whether chang
40 e level of polymorphism at the Gpdh locus in D. pseudoobscura is comparable to that found at other lo
41 conferring high or low assortative mating in D. pseudoobscura produce the same effects when inserted
44 act that this ratio is much less than one in D. pseudoobscura is also consistent with the model's pre
46 iation in crossover rate at a local scale in D. pseudoobscura, little is known about the fine-scale s
47 he sense message is transcribed similarly in D. pseudoobscura males and females and in hybrids of D.
48 anda is approximately one-quarter of that in D. pseudoobscura; mean X-linked silent diversity is abou
49 nd the antisense messages are transcribed in D. pseudoobscura, but only the sense message (TRAP100) i
50 of the higher level of sequence variation in D. pseudoobscura can be explained by differences in regi
51 the codon usage patterns in D. melanogaster, D. pseudoobscura, and D. virilis compared with the rest
53 th the completed genomes of D. melanogaster, D. pseudoobscura, and Drosophila yakuba to show that the
54 t fixed variation across the X chromosome of D. pseudoobscura because, while significant linkage dise
55 y is also present in the sequenced genome of D. pseudoobscura, and homologs have been found in Aedes
57 upport the hypothesis that the inversions of D. pseudoobscura have emerged as suppressors of recombin
59 ter, by using the public genome sequences of D. pseudoobscura and D. melanogaster and approximately 5
61 Sanger sequencing on gaps from the original D. pseudoobscura draft assembly and shown to be dependen
62 interspecific comparisons with D. simulans, D. pseudoobscura and D.virilis to explore the molecular
64 Compared to its well-studied sibling species D. pseudoobscura, D. miranda has much less nucleotide se
65 r by subjecting another polyandrous species, D. pseudoobscura, to 150 generations of experimental mon
66 D. miranda relative to its sibling species, D. pseudoobscura, suggest that it has a much smaller eff
68 proof-of-principle experiments showing that D. pseudoobscura fosmids can successfully rescue RNAi-in
69 loci surveyed are negative, suggesting that D. pseudoobscura may have experienced a rapid population
71 pose possible ancestral arrangements for the D. pseudoobscura C chromosome, which are different from
72 Additionally, many DNA duplications in the D. pseudoobscura genome are flanked by a repetitive sequ
75 onsible for recently duplicated genes in the D. pseudoobscura genome, and I observed both retroposed
76 transcriptional regulation of TRAP100 in the D. pseudoobscura lineage and that its underexpression in
77 ter for genes on this chromosomal arm in the D. pseudoobscura lineage, relative to the D. melanogaste
79 nucleotide substitutions for Acp26Aa in the D. pseudoobscura subgroup but not in the D. melanogaster
80 of indel substitution within Acp26Aa in the D. pseudoobscura subgroup were up to several times those
83 ecombination across approximately 43% of the D. pseudoobscura physical genome in two separate recombi
84 ribution of both protein-coding genes on the D. pseudoobscura neo-X chromosome and microRNA genes of
86 across each of these genomes relative to the D. pseudoobscura reference genome, and use RT-PCR to con
88 undetected losses in the lineage leading to D. pseudoobscura We find that while the original (synten
89 s strongly associated with mating success to D. pseudoobscura females, while intrapulse frequency is
90 predicted direction among 99 alleles of two D. pseudoobscura genes and five alleles of eight D. simu
91 nes within conserved regions of synteny with D. pseudoobscura had highly correlated expression; these
92 is highly variable and shares variation with D. pseudoobscura at other loci, the low level of variati
93 A large amount of variation was found within D. pseudoobscura and D. persimilis, consistent with hist
94 A survey of nucleotide polymorphism within D. pseudoobscura revealed no amino acid variation in thi
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