ライフサイエンスコーパス: DAPI

1 DAPI (4',6'-diamidino-2-phenylindole) staining of the ar

2 DAPI (4,6-diamidino-2-phenylindole) inhibited the assemb

3 DAPI also induced the directional movement of the nucleo

4 DAPI and immunofluorescence staining showed a single nuc

5 DAPI displacement is dependent on both RecA protein and

6 DAPI lifetime variations across interphase nuclei showed

7 DAPI lifetime variations in metaphase chromosome spreads

8 DAPI protected only the AT bp in the binding sites, whil

9 DAPI staining of the nucleoid demonstrated that both ori

10 DAPI uptake by HeLa cells expressing mCx30.2 was >10-fol

11 DAPI-labeled neurons at different levels of the retina w

12 he impact of three DNA-binding dyes (YOYO-1, DAPI, and DRAQ5) in a concentration-dependent manner.

13 The multicyclic dyes Hoechst 33258, DAPI, and berenil bind to TAR RNA in a similar manner an

14 he flow rate of the sample introduced with a DAPI can be much higher than that allowed with a convent

15 A laboratory-scale mass spectrometer with a DAPI-RIT (rectilinear ion trap)-DAPI configuration has b

16 a preliminary model for the enhanced action DAPI.

17 In addition, DAPI was more effective than distamycin and Hoechst 3325

18 ein synthesis), segmentation of nuclei after DAPI staining (apoptosis), and colony formation in 0.2%

19 Standard groove binding agents (DAPI, distamycin, and netropsin) showed a strong prefere

20 Also, DAPI (4',6'-diamidino-2-phenylindole) staining revealed

21 Whereas for YOYO-1 and DAPI the binding mechanisms could be assigned to bis-int

22 and M2 macrophage subtypes, alpha-actin, and DAPI was performed.

23 c cell death was detected by TUNEL assay and DAPI nuclear staining.

24 d apoptosis was evaluated by TUNEL assay and DAPI staining using digital imaging.

25 guished: DAPI-positive chromatin bridges and DAPI-negative ultrafine DNA bridges (UFBs).

26 low, Alexa Fluor(350), ethidium bromide, and DAPI, which have valences of -2, -1, +1, and +2, respect

27 n (AE1/AE3), PD-L1, CD4, CD8, CD3, CD68, and DAPI, and Panel 2 included pancytokeratin, PD-1, CD45RO,

28 PI)-sensitive foci that are bound to DNA and DAPI-insensitive foci that are DNA-less aggregates/stora

29 cleosomal fragmentation of cellular DNA, and DAPI (4',6-diamidino-2-phenylindole) staining revealed c

30 studies show that MepR binds to ethidium and DAPI with comparable affinities (K(d) = 2.6 and 4.5 micr

31 , PD-1, CD45RO, granzyme B, CD57, FOXP3, and DAPI.

32 tro by alkaline comet, DNA fragmentation and DAPI staining assays.

33 families occur in large heterochromatic and DAPI positive blocks.

34 ange, ethidium bromide, propidium iodide and DAPI staining demonstrated that cell death occurred part

35 mo-2'-deoxyuridine (BrdU) pulse-labeling and DAPI (4',6-diamidino-2-phenylindole) staining, which pre

36 ntinuous variables, replicative labeling and DAPI staining.

37 , OX40, CD27, TIM3, CD3, a tumor marker, and DAPI.

38 e two duplexes, in contrast to netropsin and DAPI, which bind with similar affinities to the two dupl

39 of PI3K signaling was examined by TUNEL and DAPI staining.

40 ng with propidium iodide, anti-Annexin V and DAPI.

41 sion of PcsB with fluorescent vancomycin and DAPI to determine patterns of cell wall synthesis and nu

42 bromide, ethidium homodimer, bis-benzimide (DAPI), Hoechst 33258 and thiazole orange] to function as

43 KD values quantitate binding effects between DAPI and the native and analogue sequences.

44 re labeled with the DNA minor groove binder, DAPI, followed by measurement and imaging of the fluores

45 API, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were measured by immunofluorescence staining; Shh,

46 d dsDNA results in displacement of the bound DAPI, producing a decrease in the observed fluorescence.

47 apoptosis by antibody to Fas was analyzed by DAPI staining and electron microscopy.

48 that contributes to minor groove binding by DAPI.

49 uclear morphologic changes of fixed cells by DAPI fluorescence microscopy and reactive oxygen species

50 AdpHyde transduced cells as demonstrated by DAPI (4', 6-diamino-2-phenylindole), TUNEL (terminal deo

51 mplete chromosome separation, as detected by DAPI-bridges and UFBs, while severe HDR disruption addit

52 went apoptosis within 48 h, as determined by DAPI staining.

53 untered by an opposite deflection induced by DAPI binding, thus effectively neutralizing intrinsic cu

54 r the RecA protein-dsDNA complex measured by DAPI displacement is 3 bp per RecA protein monomer in th

55 in livers from fat-1 animals, as measured by DAPI-staining.

56 emoved GalNAz-labeled surface cells, causing DAPI labeling (permeabilization) of underlying cells.

57 A proof-of-concept DAPI interface was designed and characterized using a mi

58 is excluded from both the XIST RNA and dense DAPI staining.

59 trates were designed with sequence-dependent DAPI-binding sites to which base excision repair glycosy

60 ,6-diamidine-2-phenylindole dihydrochloride (DAPI) and imaged with a fluorescence microscope.

61 ,6-diamidino-2-phenylindole dihydrochloride (DAPI), to detect proteinaceous and microbial contaminati

62 6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining, and phagosome-lysosome fusion was scored

63 6-diamidino-2-phenylindole, dihydrochloride (DAPI).

64 es of anaphase bridges can be distinguished: DAPI-positive chromatin bridges and DAPI-negative ultraf

65 nges of the other two ligands, using the DNA-DAPI complex as the fluorescence reporter.

66 y counterstaining the sections with DNA dye (DAPI), and cell borders can be visualized with a dye-cou

67 Cellular removal was evaluated by H&E, DAPI and DNA quantification.

68 sites since the oligo A/T binding sites for DAPI and Hoechst were centered on the same nucleotide po

69 classical metrics (qPCR, plaque assay, FVIC, DAPI) and outperformed most of them to reveal new biolog

70 BrdU/beta-tubulin/HNA/DAPI, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were measured by immunofl

71 BrdU/beta-tubulin/HNA/DAPI, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were

72 e were incubated at 4 degrees C overnight in DAPI solution.

73 let state of 4',6-diamidino-2-phenyl indole (DAPI) and its complexes with the oligonucleotides [d(CGA

74 iscontinuous atmospheric pressure interface (DAPI) has allowed the transfer of ions from atmospheric

75 iscontinuous atmospheric pressure interface (DAPI).

76 scontinuous atmospheric pressure interfaces (DAPI).

77 BPMS is mainly expressed in medium and large DAPI-, DRAQ5-, NeuroTrace- and NeuN-stained cells in the

78 DNA binding reactions involving the ligands DAPI, netropsin, lexitropsin, and the lambda repressor b

79 with minor groove and intercalating ligands: DAPI, Hoechst, and ethidium bromide.

80 irmed by serial section electron microscopy, DAPI, gammaH2AX and phospho-H3 staining.

81 via CellSearch (EpCAM(pos)/CK(pos)/CD45(neg)/DAPI(pos)) and subsequent FACS sorting.

82 al agents including distamycin A, netropsin, DAPI, Hoechst 33258, and berenil is described.

83 a-tubulin/HNA/DAPI, BrdU/GFAP/HNA/DAPI, Ngn1/DAPI, and BMP4/DAPI were measured by immunofluorescence

84 uorescently labeled with markers for nuclei (DAPI; 4',6'-diamino-2-phenylindole), endothelial cells (

85 or the lower triplet state energy, E(00), of DAPI.

86 along the circular element in the absence of DAPI but assembled onto the nonbent flanking sequence in

87 t was responsible for the enhanced action of DAPI.

88 The affinity of DAPI for the binding site decreases with the increasing

89 Trapped complexes impeded the association of DAPI in a manner dependent on the enzyme used and the lo

90 Weaker binding of DAPI to phosphate is also detected, and the triplet stat

91 The binding of RecA protein to a complex of DAPI and dsDNA results in displacement of the bound DAPI

92 dichroic components, including detection of DAPI-stained Leishmania parasite without using excitatio

93 According to the model, the short length of DAPI and its absolute specificity for A/T bps with narro

94 In addition, morphology of DAPI stained cells and DNA fragmentation assay using gel

95 hibit an increased TM due to the presence of DAPI bound in the minor groove.

96 In the presence of DAPI, most of the duplexes exhibit an increased TM due t

97 expressing mCx30.2 exhibited higher rates of DAPI uptake than did cells expressing any of the other c

98 Automated image segmentation of DAPI-stained nuclei was generated to isolate thousands o

99 heterochromatin in mouse cells and sites of DAPI-dense intranuclear heterochromatin in human and ham

100 The dendritic trees of DAPI-3 cells, which range from about 150 microm up to ab

101 In Ca(2+)-free medium, uptake of DAPI by HeLaCx30.2-EGFP cells was increased approximatel

102 The use of DAPI provides a simple solution to the problem of coupli

103 and classifying egg chamber stages based on DAPI images.

104 staining and counterstaining with Hoechst or DAPI.

105 amples were mounted with propidium iodide or DAPI.

106 the binding to either duplex of netropsin or DAPI induces little or no change in the electrophoretic

107 CN(-) signals overlapped DAPI fluorescence signals corresponding to nuclei.

108 ',6-diaminidin-2-phenylindoldihydrochloride (DAPI).

109 arly show that 4,6-diamidino-2 phenylindole (DAPI) is superior to both of these drugs at negating the

110 s stained with 4,6-diamidino-2-phenylindole (DAPI) [5].

111 estriction of 4',6-diamidino-2-phenylindole (DAPI) binding to packaged DNA.

112 ingrowth with 4',6'-diamino-2-phenylindole (DAPI) filters.

113 Staining with 4',6-diamidino-2-phenylindole (DAPI) indicated that poly- phosphate (polyP) was prefere

114 -groove-bound 4',6-diamidino-2-phenylindole (DAPI) originates from an intricate interplay between the

115 junction with 4'6'-diamidino-2-phenylindole (DAPI) staining and by fluorescence staining of the nucle

116 gmentation by 4, 6-diamidino-2-phenylindole (DAPI) staining.

117 ide (PI) and 4', 6-diamidino-2-phenylindole (DAPI) staining.

118 6-Diamidino-2-phenylindole (DAPI) was used for nuclear counterstaining.

119 netropsin and 4,6-diamidino-2-phenylindole (DAPI), and a DNA hairpin having the sequence 5'-d(CGAATT

120 of cells with 4,6-diamidino-2-phenylindole (DAPI), and the polyP-binding domain of Escherichia coli

121 toxin (Cdt), 4',6-diamidino-2-phenylindole (DAPI), human gingival epithelial cells (HGEC), human gin

122 tissue (CT), 4',6-diamidino-2-phenylindole (DAPI), human gingival epithelial cells (HGEC), human gin

123 in vitro with 4',6-diamidino-2-phenylindole (DAPI), intracellular injection with Lucifer Yellow, and

124 4',6-diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binde

125 equally with 4',6-diamidino-2-phenylindole (DAPI), suggesting that byr4 is required for proper karyo

126 netropsin and 4',6-diamidino-2-phenylindole (DAPI), two AT-specific minor groove binding ligands that

127 rescence of a 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complex upon RecA protein binding.

128 uorescence of 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complexes.

129 been defined: 4,6-diamidino-2-phenylindole (DAPI)-sensitive foci that are bound to DNA and DAPI-inse

130 iodide (PI) or 4',6'-diamino-2-phenylindole (DAPI)-stained cells.

131 ur results from 4, 6-diamino-2-phenylindole (DAPI)-stained spreads showed that the "synizetic knot",

132 ton imaging of 4',6'-diamino-2-phenylindole (DAPI)-stained tissue to quantify neuron loss in postmort

133 e stained with 4',6'-diamino-2-phenylindole (DAPI).

134 h the DNA dye 4',6-diamidino-2-phenylindole (DAPI).

135 ide (TP3) and 4',6-diamidino-2-phenylindole (DAPI).

136 meable dye and 4',6'-diamino-2-phenylindole (DAPI).

137 ide (PrI) and 4'-6-diamidino-2-phenylindole (DAPI).

138 roove binder, 4',6-diamidino-2-phenylindole (DAPI).

139 inding ligand 4',6-diamidino-2-phenylindole (DAPI).

140 roplasts with 4',6-diamidino-2-phenylindole (DAPI); staining at the single-molecule level with ethidi

141 py genes, and 4'-6-diamidino-2-phenylindole (DAPI-) and G-banded chromosomes and report nonrandom cyt

142 cidification by application of CO(2) reduced DAPI uptake by HeLaCx30.2-EGFP cells but had little effe

143 ement of signals relative to high-resolution DAPI or G-bands.

144 ified on the basis of morphology and reverse DAPI (rDAPI) banding.

145 arkers for incomplete chromosome separation: DAPI-bridges and Ultra-fine bridges (UFBs).

146 in, showed statistically significant shorter DAPI lifetime values than the rest of the chromosomes.

147 ferentially localized to regions with strong DAPI signals.

148 c effects of nuclear stains, including SYTO, DAPI dilactate, Hoechst 33342, and FITC dyes upon the ph

149 The DAPI potentially would allow a significant enhancement t

150 Differences in the DAPI lifetimes for the heteromorphic regions suggest dif

151 This target is the DAPI-3 cell.

152 The simplicity of application of the DAPI for performing ion/molecule and ion/ion reactions h

153 ase in the zero-field splitting (zfs) of the DAPI triplet state.

154 The perikarya of the DAPI-3 cells are found in the proximal inner nuclear lay

155 Because the cell bodies of the DAPI-3 cells are the only ones in the inner nuclear laye

156 t on the enzyme used and the location of the DAPI-binding site in relation to the lesion.

157 RNA territory, which resides outside of the DAPI-dense Barr body.

158 of a standard Frenkel exciton theory of the DAPI-DNA interactions.

159 lesser extent, UTP and dCTP also support the DAPI displacement reaction, but dGTP, GTP, dITP and TTP

160 Thus, we have shown that the DAPI-3 cells contain the highest concentrations of the a

161 In contrast to the DAPI-3 cell, we have also shown that the starburst amacr

162 ABA(A) receptor subunits is localized to the DAPI-3 type amacrine cell.

163 pray ionization source synchronized with the DAPI has been implemented to improve the sample usage ef

164 The pressure variation associated with the DAPI operation was used to turn on and off the synchroni

165 for mutant RecA proteins, suggest that this DAPI displacement assay monitors formation of the high a

166 meter with a DAPI-RIT (rectilinear ion trap)-DAPI configuration has been developed to explore this po

167 d rats transfected with hCx31.9-EGFP took up DAPI and ethidium bromide 5-10 times faster than wild-ty

168 crophages were scored for apoptosis by using DAPI (4',6-diamindino-2-phenylindole dihydrochloride) an

169 ometry, as well as chromosome counting using DAPI staining and fluorescence in situ hybridization, re

170 155 fluorescent dye molecules per CPMV using DAPI (4',6-diamidino-2-phenylindole dihydrochloride), pr

171 ned for nucleus and actin distribution using DAPI and phalloidin respectively.

172 referentially localized to regions with weak DAPI signals.

173 TP3 binds DNA by intercalation, whereas DAPI exhibits minor groove binding.

174 methyl groups exhibit very poor binding with DAPI, while those containing a single fluorine behave es

175 relocalization of C/EBP beta coincides with DAPI staining of heterochromatin.

176 to 16 fluorescent foci that colocalize with DAPI (4',6'-diamidino-2-phenylindole)-positive material

177 neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluoresenc

178 cent digital images of sections stained with DAPI and antibodies directed against GS-NEM, glial fibri