ライフサイエンスコーパス: DEF

10 s and gene activity interact, we exploited a DEF allele which carries a transposon insertion in the M

11 lly assembled on the galactose residues of a DEF chimera.

12 is dependent on DEF-1 GAP activity, since a DEF-1 mutant lacking the GAP domain failed to stimulate

13 80 microM observed for the natural activator DEF.

14 not differ significantly between the ABC and DEF groups (mean [SD], -1.19 [0.49] vs -1.17 [0.48] mug/

15 purified mutant ELK1 in which the D-box and DEF motifs were disrupted did not bind AR.

16 ts showed impaired performance while CON and DEF rats were unaffected.

17 tes can be classified into DEF-dependent and DEF-independent substrates.

18 ovel LFY/FLO paralogs, here we clone FLO and DEF duplicates from additional Lamiales taxa and apply c

19 for preservation of co-orthologs of FLO and DEF in Lamiales, including interactions among the genes

20 Exposure to PBO and DEF resulted in a broad transcriptional response and abo

21 Similarly, PLE and DEF/GLO induce DNA bending although the direction of the

22 NbDEF is a functional homolog of Antirrhinum DEF.

23 fied duplicates of the genes LFY/FLO and AP3/DEF that directly interact in a floral regulatory pathwa

24 h duplicates of MADS box genes including AP3/DEF are common throughout the angiosperm lineage, LFY/FL

25 from a general increase in cell motility, as DEF-1-expressing cells also exhibit enhanced levels of b

26 dy the function of the Nicotiana benthamiana DEF ortholog (NbDEF).

27 rs, which partially inhibit p38alpha binding DEF-dependent substrates, whereas maintaining its other

28 Moreover, both DEF and PBO induced genes that are strongly implicated i

29 leotide substitutions for one (FLO) or both (DEF) paralogs that appears to be due to relaxed purifyin

30 Most importantly, free and RI-bound DEF-RNase A have pKa values of 6.68 and 7.29, respective

31 g that spreading and motility are altered by DEF-1 through different pathways.

32 In contrast, protection by DEF binding to ERK2 revealed a distinct hydrophobic pock

33 val of the formyl group in these proteins by DEF would explain the rescue of the slow-growth phenotyp

34 ontrast, the inhibition of cell spreading by DEF-1 was not dependent on GAP activity, indicating that

35 t switch, where GAL4 DBD was fused to CfEcR (DEF) and VP16 AD was fused to MmRXR (EF) was found to be

36 luble, dimeric peptide MHC class II chimera (DEF) induces Ag-specific T cell anergy.

37 mpatibility complex (pMHC) class II chimera (DEF) to prediabetic double-transgenic mice prevents the

38 trol diet (CON) or one deficient in choline (DEF) during embryonic days 12-17 were given multiple inj

39 hat were given a deficient level of choline (DEF=0.0 g/kg), sufficient choline (CON=1.1 g/kg) or supp

40 ignificantly, several protooncogenes contain DEF domains and are regulated by ERK1/2.

41 1/2)-MAPKs localize to effectors containing DEF (docking site for ERK, (F)/(Y) -X-(F)/(Y) -P)- or D-

42 MAPK interaction with substrates containing DEF sites.

43 selection--these include the major defensin (DEF) gene cluster and MCPH1, a gene that may have contri

44 of floral homeotic genes such as DEFICIENS (DEF) in non-model systems.

45 We isolated PTD, a member of the DEFICIENS (DEF) family of MADS box transcription factors, from the

46 The DEFICIENS (DEF) gene is required for establishing petal and stamen

47 ere exposed to a choline chloride deficient (DEF), sufficient (CON), or supplemented (SUP) diet durin

48 sion of the E. coli polypeptide deformylase (DEF), which removes the formyl group from the N-terminal

49 2',7'-Diethylfluorescein (DEF) has spectral properties similar to those of fluores

50 pyrazolyl) (H2BDP) in N,N'-diethylformamide (DEF) at 130 degrees C generates the metal-organic framew

51 H(2)BDC), and occluded N,N-diethylformamide (DEF) (if any) gave values of 78.64 +/- 2.95 and 99.47 +/

52 Similar reaction in N,N-diethylformamide (DEF) at higher temperatures resulted in a porous, 3-D fr

53 Mn(NO(3))(2).4H(2)O in N,N-diethylformamide (DEF) produces the two-dimensional framework solids Mn(3)

54 ylformamide (DMF), and N,N-diethylformamide (DEF), we can observe, intercept, and isolate, the import

55 have established that a IVa2 protein dimer (DEF-B) binds specifically to an intragenic ML promoter s

56 Here we report that ectopic DEF-1 enhances cell migration toward PDGF as well as IGF

57 to an altered binding of the nonreducing-end DEF trisaccharide to the native serpin conformation.

58 Therefore, disrupting ERK-DEF domain interactions could be an alternative to inhib

59 ERK2 DEF mutants undergo regulated nuclear translocation but

60 Thus, an apparent critical role for ERK2 DEF motif signaling during tumorigenesis is the regulati

61 In a second experiment, DEF, CON, and SUP rats were either maintained on a contr

62 ated whether NIH 3T3 cells stably expressing DEF-1 have altered cell motility.

63 ar factor, digestive organ expansion factor (DEF), has a key role in ribosome biogenesis, functioning

64 tinin (HA)110-120/I-E(d)alphabeta/Fcgamma2a (DEF).

65 We present an automated dead-end filling (DEF) approach, which is derived from the wisdom of endos

66 Consistent with these findings, DEF is highly expressed in human neuroblastoma, and its

67 Further evidence for a role for DEF-1 in adipogenesis was provided by heightened express

68 DEJL motifs), RSK-1 (DEJL motif), and c-Fos (DEF motif) with K(D) values of 0.25, 0.15, and 0.97 muM,

69 Recovery from DEF anergy occurred late and spontaneously at the expens

70 receptor constructs were prepared by fusing DEF domains (hinge region plus ligand-binding domain) of

71 , several constructs were prepared by fusing DEF domains of Choristoneura fumiferana EcR (CfEcR), C.

72 (D-domain) and a docking site for ERK, FXF (DEF) motif (F-site), respectively.

73 ntain functional docking site for ERK, FXFP (DEF) domains that serve to locally concentrate the activ

74 equired the hBVR docking site for ERK, FXFP (DEF, C-box) and D(delta)-box (ILXXLXL) motifs.

75 ng proteins contain amino acid motifs, e.g., DEF or D-motifs, which regulate docking with ERK1/2.

76 However, DEF-1 mRNA was detected in multiple tissues, suggesting

77 ral (HSET), and other genes (TULP1, HSPRARD, DEF-6, EO6811, cyclophilin), as well as a number of RP g

78 tahedral SBUs found in Zn(2)(NDC)(3).[(HTEA)(DEF)(ClBz)](2) (MOF-37) form the most common structure f

79 ectly accessing the chloropeptin I versus II DEF ring system as well as key unnatural isomers, its ut

80 a decrease in dentate cell proliferation in DEF rats only.

81 Variations in DEF site sequence requirements provide one possible mech

82 g p38alpha substrates can be classified into DEF-dependent and DEF-independent substrates.

83 ogous substrate used to provide the isolated DEF ring system in our first-generation approach.

84 d to a choline-supplemented diet (5.0 g/kg), DEF rats' performance was significantly impaired while C

85 Physiological ligands like DEF chimeras may provide new tools for silencing the aut

86 In addition, mutating DEF pocket residues decreased the autophosphorylation ca

87 basepairs) which eukaryotes require obeys N(DEF)=1/2 (N(C)/N(P)) (N(C)-N(P)), where N(C) is the amou

88 This value N(DEF) corresponds to a few percent of the genome in Homo

89 h D domains, prevents RSK activation but not DEF-domain signaling.

90 -domain binding pocket prevent activation of DEF-domain-containing effectors but not RSK (90 kDa ribo

91 ing an assay for the DNA-binding activity of DEF-A, we identified the unknown protein by using conven

92 Administration of DEF in vivo induced differentiation of resting and activ

93 Administration of DEF-gal-Dox to mice expressing the TCR-HA transgene redu

94 moter with the specificity characteristic of DEF-A and stimulates transcription from the ML promoter

95 l L4 33-kDa protein is the only component of DEF-A: the IVa2 and L4 33-kDa proteins are necessary and

96 The antidiabetogenic effects of DEF rely on the induction of anergy in splenic autoreact

97 The recalls of reactions and dead ends of DEF reach around 73% and 86%, respectively.

98 Expression of DEF in a subset of meristem layers gives rise to organs

99 of these chimeras we show that expression of DEF in L1 makes a major contribution to morphology in wh

100 Ectopic expression of DEF-1 in fibroblasts resulted in the differentiation of

101 sis was provided by heightened expression of DEF-1 mRNA in adipose tissue isolated from obese and dia

102 bly because of a non-autonomous inhibitor of DEF activity in this whorl.

103 s, reflecting altered patterns and levels of DEF gene activity.

104 ereas, coexpression of an inactive mutant of DEF does not.

105 Since transient overexpression of DEF-1 has been shown to interfere with focal adhesion fo

106 Recombinant proteins containing the RRMs of DEF-3(g16/NY-LU-12) and LUCA15 specifically bound poly(G

107 a from Hu syndrome patients with the RRMs of DEF-3(g16/NY-LU-12) and LUCA15.

108 ression patterns are more similar to that of DEF and AP3 than to other members of the TM6 subfamily.

109 he increase in cell motility is dependent on DEF-1 GAP activity, since a DEF-1 mutant lacking the GAP

110 eu (DEJL) and a second containing Phe-X-Phe (DEF).

111 such as S,S,S-tributyl phosphorotrithioate (DEF), diethyl maleate (DEM), piperonyl butoxide (PBO) an

112 ch proteins in Antirrhinum majus, SQUA, PLE, DEF and GLO.

113 defined as the DEF site interaction pocket (DEF pocket), is formed subsequent to ERK2 and p38alpha a

114 L) (amino acids 41-97) harbors two potential DEF-type ERK1/2 kinase-docking domains, DEF1 and DEF2.

115 We have purified and cloned a novel protein, DEF-1 (differentiation-enhancing factor), from bovine br

116 We show that MAPK isoforms recognize DEF sites with unique sequences and identify two key res

117 An improved synthesis of rings DEF of solanoeclepin A has been achieved from ent-Hajos

118 Mutating selected DEF pocket residues significantly decreased the phosphor

119 a platform for designing p38alpha-selective DEF site blockers, which partially inhibit p38alpha bind

120 ocess allowed access to the first simplified DEF ring analogues of the kibdelones.

121 -activated receptor gamma (PPARgamma), since DEF-1 NIH 3T3 cells demonstrated augmented levels of PPA

122 esponse occurred upon interaction of soluble DEF with TCR and CD4 molecules followed by early activat

123 Independent of antigen processing, soluble DEF was almost 2 logs more potent in stimulating cognate

124 ave previously demonstrated that the soluble DEF molecule binds stably and specifically to HA110-120-

125 nsically active p38alpha mutants, suggesting DEF-mediated trans-autophosphorylation in p38alpha.

126 Perhaps somewhat surprisingly, DEF rats were not more susceptible to seizure induction

127 ional infected-cell-specific protein, termed DEF-A, to the promoter.

128 cell-based ARF GAP assay to demonstrate that DEF-1 functions as a GAP for ARF1 and not ARF6 in vivo.

129 At 70 days of age, we found that DEF and SUP rats required fewer choices to locate 8 bait

130 Our results indicate that DEF and possibly other components of the SSU processome

131 These results suggest that DEF-1 is an important component of a signal transduction

132 This suggests that DEF-1 alters cell motility through the deactivation of A

133 The DEF-gal-Dox construct preserved both the binding capacit

134 ERK-docking sites in ELK1, the D-box and the DEF (docking site for ERK, FXFP) motif, as the essential

135 ining the DNA-binding domain of GAL4 and the DEF segment of TR2-11.

136 ion between the ABC-flavanone moiety and the DEF-flavone moiety, as well as the electronic transition

137 A second docking site, defined as the DEF site interaction pocket (DEF pocket), is formed subs

138 ntrast to HA110-120 peptide presented by the DEF molecule to T cells, the nominal synthetic peptide i

139 thrombin conformation that is induced by the DEF trisaccharide.

140 n alternative mode of action mediated by the DEF-binding pocket (DBP) of ERK.

141 Mutations in the DEF-domain binding pocket prevent activation of DEF-doma

142 een to identify sequence requirements of the DEF site (docking site for ERK FXF), a docking motif sep

143 largely uncharacterized TM6 subfamily of the DEF/APETELA3(AP3)/TM6 group, its spatio-temporal express

144 d of intrinsically active mutants showed the DEF pocket, giving motivation for studying its role in s

145 ive silencing activity is encoded within the DEF segment of the receptor molecule, as evidenced by th

146 ety and the electronic transition within the DEF-flavone moiety, while another diagnostic positive CE

147 well as the electronic transition within the DEF-flavone moiety.

148 Thus, DEF-3(g16/NY-LU-12) and LUCA15 represent members of a no

149 Brief exposure of HA-specific T cells to DEF-gal-Dox construct in vitro was followed by drug inte

150 In contrast to DEF, a combination of TCR and CD4 mAbs did not induce su

151 ure, but converts back to 1 upon exposure to DEF, consistent with the presence of a flexible framewor

152 Remarkably, ERK2 signaling to DEF motif-containing targets is required to induce EMT a

153 degree of substrate preference was unique to DEF-1, as other ARF GAP proteins, ACAP1, ACAP2, and ARFG

154 nteraction profile for ECS and trisaccharide DEF.

155 as previously shown to involve trisaccharide DEF first binding and inducing activation of the serpin,

156 M) comparable to the reference trisaccharide DEF ( approximately 4.5 microM), it accelerated the inhi

157 nding domain for the reference trisaccharide DEF.

158 mimic the nonreducing end trisaccharide unit DEF of the sequence specific heparin pentasaccharide DEF

159 Using DEF-RNase A rather than fluorescein-RNase A in a micropl

160 After 12 weeks, DEF rats were significantly impaired by choline suppleme

161 the signal transduction pathway(s) in which DEF-1 is involved is not limited to adipogenesis.

162 to the approximately 300-fold observed with DEF.