ライフサイエンスコーパス: DGGE

1 DGGE amplicons with homology to Staphylococcus sp. (8/13

2 DGGE and 454-pyrosequencing of PCR-amplified 16S rRNA ge

3 DGGE band sequencing revealed the presence of taxa with

4 DGGE profiles from samples collected at different time p

5 DGGE profiles indicate that dogs have a highly diverse m

6 Prokaryotic 16 S rRNA gene was amplified and DGGE was performed.

7 ated support the utility of both culture and DGGE for the microbial characterization of chronic wound

8 erent species of Nitrospira spp. detected by DGGE and sequencing analysis.

9 ven (85%) species could be differentiated by DGGE.

10 exception of sequences indistinguishable by DGGE from seven infants.

11 While a majority of skin-derived DGGE consortial fingerprints could be differentiated fro

12 s), denaturing gradient gel electrophoresis (DGGE) (34 cases) or a yeast-based truncation assay (110

13 Denaturing Gradient Gel Electrophoresis (DGGE) analysis confirmed the above macroscopic observati

14 ing denaturing gradient gel electrophoresis (DGGE) and the cloning/sequencing approach.

15 the denaturing gradient gel electrophoresis (DGGE) component of this system strongly depends on the d

16 and denaturing gradient gel electrophoresis (DGGE) for K-ras mutations.

17 PCR-denaturing gradient gel electrophoresis (DGGE) in conjunction with DNA sequencing.

18 Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to dif

19 Denaturing gradient gel electrophoresis (DGGE) of the class IIB loci was used to test the efficac

20 rom denaturing gradient gel electrophoresis (DGGE) shifted more strongly with time than in response t

21 the denaturing gradient gel electrophoresis (DGGE) system combined with sequencing to characterize th

22 ive denaturing gradient-gel electrophoresis (DGGE) system.

23 the denaturing gradient gel electrophoresis (DGGE) technique has been appropriately modified for simu

24 Denaturing gradient gel electrophoresis (DGGE) with 16S rDNA primers generally indicated that mic

25 ts, Denaturing Gradient Gel Electrophoresis (DGGE), short-chain fatty acid (SCFA) and ammonium analys

26 Denaturing gradient gel electrophoresis (DGGE), which compared the overall microbial profiles, sh

27 ing denaturing gradient gel electrophoresis (DGGE).

28 by denaturing gradient gel electrophoresis (DGGE).

29 led denaturing gradient gel electrophoresis (DGGE).

30 by denaturing gradient gel electrophoresis (DGGE).

31 This study examined DGGE-generated diversity profiles of cultivable bacteria

32 Sequence analysis of excised DGGE bands consisted of 2.7 phylotypes, on average.

33 Sequencing of bands from DGGE profiles and intact polar lipid analyses were in ac

34 ongly suggest that the anomalous behavior in DGGE of tRNA gene-containing mtDNA fragments reflects th

35 Differences in DGGE profiles were distinguished on the basis of a clust

36 here was a significantly higher variation in DGGE profiles between different dogs than between duplic

37 those identified by the culture independent DGGE analysis.

38 Fluorescence microscopy, DGGE and RFLP analysis of PCR amplified16S rRNA genes, a

39 ll intestine were evaluated by comparison of DGGE profiles from different time points within the same

40 cient; 100% represents complete identity) of DGGE profiles from group 1 dogs.

41 The reproducibility of DGGE profiles and variations in bacterial diversity betw

42 mean (+/- standard deviation) similarity of DGGE profiles of duodenal juice between the dogs in grou

43 analysed by direct nucleotide sequencing or DGGE, including a non-conservative amino acid substituti

44 grading SDIMO genes were widespread, and PCR-DGGE analysis showed that group-5 SDIMOs were present in

45 on conventional isolation techniques and PCR-DGGE-based methods in different chestnut-based sourdough

46 the Illumina MiSeq platform, as well as PCR-DGGE followed by band sequencing.

47 Additional types detected by PCR-DGGE were found in 14 (63.6%) of the 22 infants.

48 nt study uncultured bacteria detected by PCR-DGGE were no more frequent in fecal samples from infants

49 on of fermentative bacteria observed (by PCR-DGGE) in R2.

50 denaturing gradient gel electrophoresis (PCR-DGGE) surveys microbial diversity by displaying PCR-gene

51 denaturing gradient gel electrophoresis (PCR-DGGE), allowing the DNA from uncultured bacteria to be i

52 Initially, we developed a set of PCR-DGGE running conditions appropriate to oral bacteria.

53 Overall diversity using PCR-DGGE did not yield any pathogenic sequence matches even

54 rodiversity analysis was conducted using PCR-DGGE, targeting Escherichia coli.

55 h PCR-DGGE was 10.1 per infant, of which PCR-DGGE contributed 10.4% of the types identified.

56 types detected by culture combined with PCR-DGGE was 10.1 per infant, of which PCR-DGGE contributed

57 Very different 16S rDNA DGGE banding profiles were obtained when replicate cv. V

58 se culture-independent molecular techniques (DGGE and clone libraries) to characterize ciliate and ba

59 The DGGE obtained showed changes in the lactobacilli communi

60 ng individuals was directly reflected in the DGGE patterns.

61 According to DGGE analyses, all wounds contained significantly greate

62 from Citrus sinesis (cv. Valencia) by using DGGE analysis followed by cloning and sequencing of the