ライフサイエンスコーパス: DMEM

1 ecco's minimum essential medium-Hanks' F-12 (DMEM-F-12) medium with and without exogenous hormone sup

2 Fifteen LP-BM5 and 15 DMEM mice were trained in avoidance 7 wk after inoculati

3 to -196 degrees C), or Vitro (Placed in 40% DMEM and rapidly cooled to -196 degrees C).

4 In a DMEM medium with 6% horse serum, the TSR was increased b

5 was enhanced by pyruvate, high glucose, and DMEM.

6 s examined by switching KSFM to DMEM/ITS and DMEM/10% FBS.

7 ured in a serum-free keratinocyte medium and DMEM/F12 medium containing 10% FBS in a 37 degrees C inc

8 ced within 30 min of refeeding with complete DMEM.

9 lls were exposed to hyperosmolar conditions (DMEM-F12 culture medium plus 50 mM NaCl or 100 mM mannit

10 uman aqueous humor (DMEM-AH), heat-denatured DMEM-AH, 10% fetal bovine serum (DMEM-FBS, the standard

11 ndard culture supplement), or heat-denatured DMEM-FBS.

12 ent, and then cultured until day 4 in either DMEM/F-12 alone, to retain the keratocyte phenotype, or

13 ulbecco's modified Eagle's medium/Ham's F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (R

14 human LECs were cultured in standard 10% FCS-DMEM containing various concentrations of sugar.

15 designated phenotypes: baseline fibroblasts (DMEM with 10% serum), activated fibroblasts (10% serum+b

16 s were randomly injected with the following: DMEM (G-1), BMSCs (G-2), and preconditioned BMSCs (G-3).

17 DMEM, with or without 10% FBS, in serum-free DMEM containing insulin-transferrin-sodium selenite (ITS

18 sue pieces were also incubated in serum-free DMEM for 48 h at 37 degrees C in 95% air/5% CO(2) to det

19 lture plates and then cultured in serum-free DMEM, with and without LEDGF.

20 human donor eyes and cultured in serum-free DMEM.

21 rol cultures were exposed only to serum-free DMEM.

22 All cytokines were diluted in serum-free DMEM.

23 RCKs cultured in serum-free DMEM/F12 without frequent changes of medium maintained t

24 in our "cultures" was transferable to fresh DMEM, molecular examination of decalcified biofilms fail

25 The results favor the use of high-glucose DMEM with pyruvate for future RPE differentiation studie

26 M supplemented with 50% human aqueous humor (DMEM-AH), heat-denatured DMEM-AH, 10% fetal bovine serum

27 In DMEM, cells from TZ showed higher colony-forming efficie

28 In DMEM, the binding efficiency of 633 decreased significan

29 In DMEM-AH-conditioned medium, myocilin expression was incr

30 by digestion in 1 mg/mL of collagenase A in DMEM and seeded on plastic or the stromal matrix of huma

31 al matrix of human amniotic membrane (AM) in DMEM with different concentrations of FBS.

32 plastic or amniotic membrane matrix (AM) in DMEM, with or without 10% FBS, in serum-free DMEM contai

33 epithelial cells frozen at -80 degrees C in DMEM-F-12 medium with 10% dimethyl sulfoxide for several

34 A549 cells in DMEM/5% FCS BMP-2 activated Smad-1/5 and caused a transi

35 broblast overgrowth (D-valine), contained in DMEM.

36 neralization studies, cells were cultured in DMEM containing 10% FBS and a) vehicle only; b) ascorbic

37 uggest that OMECs from LL and TZ cultured in DMEM give rise to undifferentiated cells with high growt

38 ated from mouse bone marrow were cultured in DMEM supplemented with 10% fetal bovine serum, cell popu

39 ne was measured in ARPE-19 cells cultured in DMEM-F12 medium without or with the addition of 50 mM Na

40 an corneal epithelial cells were cultured in DMEM/F12 medium containing 10% FBS and 5 microg/mL insul

41 neal epithelial (HCE) cells were cultured in DMEM/F12 medium containing 10% FBS in a 37 degrees C inc

42 n pairs on Millipore filters and cultured in DMEM/F12 medium.

43 /mm2), and high (500 cells/mm2) densities in DMEM/F12 containing insulin, transferrin, selenium, and

44 for 24 hours with control (0.015% ethanol in DMEM) or the free acid forms of bimatoprost (0.01 or 0.1

45 a laboratory chamber system and extracted in DMEM.

46 VEGF secretion was greater in DMEM cultures and was affected by glucose but not by pyr

47 l markers were detected in cultures grown in DMEM and RPMI.

48 CBCEPCs were grown in DMEM containing 2 ng/ml fibroblast growth factor and 6%

49 explants and the acinar cells were grown in DMEM supplemented with 10% fetal bovine serum.

50 becular meshwork cell cultures were grown in DMEM supplemented with 50% human aqueous humor (DMEM-AH)

51 n did the more spindle-shaped cells grown in DMEM-FBS.

52 at 200 cells/mm(2), and cells were grown in DMEM/F12 containing (1) 10% FBS or (2) 10% FBS with FGF

53 It is grown in DMEM/F12 medium as standard, but its phenotype is depend

54 4,000-35,000) from hyperapoB cells, grown in DMEM/F12 medium without BP, had significantly decreased

55 were serum starved by growth for 48 hours in DMEM/F12 with 0.2% FBS and subsequently were either scra

56 ased outflow resistance in eyes incubated in DMEM, but only if myocilin was preincubated with porcine

57 ell lysates isolated from cells incubated in DMEM-AH differed from those incubated in DMEM-FBS.

58 also altered: Trabecular cells incubated in DMEM-AH showed larger-, broader-, and flatter-appearing

59 in DMEM-AH differed from those incubated in DMEM-FBS.

60 l compartment pH) after 4-hour incubation in DMEM were measured.

61 lated from FBS after prolonged incubation in DMEM.

62 was observed beyond the normal induction in DMEM-AH.

63 re cultured on filters for up to 3 months in DMEM/F12 or DMEM media with or without pyruvate and 1% f

64 Pigmentation never occurred in DMEM/F12.

65 e, to retain the keratocyte phenotype, or in DMEM containing 10% fetal bovine serum, to cause the ker

66 with 10, 100, and 1000 pg/mL TGF-beta1 or in DMEM with 1% FBS and 10 ng/mL TGF-beta1.

67 d throughout the exponential growth phase in DMEM medium.

68 ratocytes from bovine corneas were plated in DMEM/F-12 containing 1% horse plasma, to allow cell atta

69 eous humor (78% +/- 77% when preincubated in DMEM containing porcine aqueous humor versus 13% +/- 15%

70 a reduced form of the folic acid present in DMEM.

71 l; (b) autoclaving 10 and 100 ng/ml ricin in DMEM at 121 degrees C for 30 min completely abolished ac

72 of 0.45-microm membrane-filtered samples in DMEM.

73 Our results from 4-week studies in DMEM without cells (group 1), SMs (group-2), SiPS (group

74 mental biology medium 402 (MCDB 402) than in DMEM.

75 yrosine derivative, 3-Nitro-L-tyrosine, into DMEM can mitigate the degradation of PSM at 8 degrees C

76 were incubated in three cell culture media (DMEM, RPMI-1640, and DCCM-1) to examine the impact of Ag

77 ained for 2 to 3 months in the basal medium (DMEM containing 2% fetal bovine serum) with one medium c

78 jection of Dulbecco's modified Eagle medium (DMEM) (G5).

79 ced in 10% Dulbecco's modified Eagle medium (DMEM) and slowly frozen to -196 degrees C), or Vitro (Pl

80 ia such as Dulbecco's modified eagle medium (DMEM) or simulated body fluid.

81 th (LB) or Dulbecco's modified Eagle medium (DMEM), and transcription was examined during different p

82 evident in Dulbecco's modified Eagle medium (DMEM), but not in RPMI.

83 onine-free Dulbecco's Modified Eagle Medium (DMEM).

84 pplemented serum-free and lipid-free medium (DMEM/F12).

85 lture in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum.

86 standard Dulbecco's modified Eagle's medium (DMEM) containing 15% fetal bovine serum.

87 astic in Dulbecco's modified Eagle's medium (DMEM) containing insulin-transferrin-sodium selenite (IT

88 0 microL Dulbecco's modified Eagle's medium (DMEM) or 1.5 x 10(7) bone marrow-derived mononuclear cel

89 h either Dulbecco's modified Eagle's medium (DMEM) or DMEM supplemented with 50% porcine aqueous humo

90 tured in Dulbecco's modified Eagle's medium (DMEM) plus 2% fetal bovine serum (FBS) or 2% FBS plus EM

91 tured in Dulbecco's modified Eagle's medium (DMEM), with and without fetal bovine serum (FBS).

92 medium (Dulbecco's modified Eagle's medium [DMEM]) containing 1% antibiotic/antimycotic solution and

93 (BMC) from isogenic donor rats in 70 microL DMEM.

94 ncubated for 1 h at 37 degrees C in 20 ml of DMEM containing 0.2% (wt/vol) collagenase.

95 For in vivo studies, 70 muL of DMEM without cells (group 1) or containing 1.5x10(6) (Nu

96 ic strength of RPMI was increased to that of DMEM.

97 ast, the incubation of AgNWs in RPMI-1640 or DMEM did not lead to sulfidation.

98 Dulbecco's modified Eagle's medium (DMEM) or DMEM supplemented with 50% porcine aqueous humor.

99 on filters for up to 3 months in DMEM/F12 or DMEM media with or without pyruvate and 1% fetal calf se

100 nced green fluorescent protein (EGFP)-GRP or DMEM grafts.

101 At a paper: DMEM ratio of 50 mug/ml, cell viability in both cell lin

102 e Hep II domain, and the fellow eye received DMEM or a heat-denatured Hep II domain.

103 In contrast to RPMI, DMEM both expressed the putative stem cell marker Bmi-1

104 pieces of sclera were cultured in low-serum DMEM/F-12 media.

105 abilization reagent or cultured in low-serum DMEM/F-12 medium.

106 t-denatured DMEM-AH, 10% fetal bovine serum (DMEM-FBS, the standard culture supplement), or heat-dena

107 lement (DMEM/ITS) or 10% fetal bovine serum (DMEM/10% FBS), or in a defined keratinocyte serum-free m

108 Cells grown in standard DMEM containing 25 mM glucose were treated with diamide,

109 ransferrin-sodium selenite (ITS) supplement (DMEM/ITS) or 10% fetal bovine serum (DMEM/10% FBS), or i

110 1-, or fetal bovine serum (FBS)-supplemented DMEM/F12 medium.

111 Daily administration of ascorbic acid to DMEM-AH failed to increase myocilin expression beyond th

112 the astrocyte culture medium was changed to DMEM containing various concentrations of 8-CPT-cAMP, a

113 phenotype was examined by switching KSFM to DMEM/ITS and DMEM/10% FBS.

114 that folds and self-assembles in response to DMEM resulting in mechanically rigid hydrogels.

115 fluence in serum-free medium and switched to DMEM/F12 with 10% serum to promote differentiation.

116 inoculated with LP-BM5; 30 received vehicle (DMEM) injections.

117 nischemic legs) and between subjects (BMC vs DMEM treatment) were significant for total blood vessel

118 Pyruvate, in combination with DMEM, induced dark pigmentation and promoted differentia

119 reased cell proliferation when compared with DMEM-FBS (11% vs. 141%).

120 ficantly downregulated in KSFM compared with DMEM/10% FBS.

121 e in standard organ culture dishes, fed with DMEM/F12 plus 2% B-27 supplement and 1% fetal bovine ser

122 yocilin expression beyond that obtained with DMEM-AH.

123 or versus 13% +/- 15% when preincubated with DMEM alone, n = 6, P = 0.03).

124 Trabecular cells supplemented with DMEM-AH for 21 days showed decreased cell proliferation

125 oned medium isolated from cells treated with DMEM-FBS (442%), but only a 10% increase in myocilin was

126 with that in anterior segments treated with DMEM.