ライフサイエンスコーパス: DMH

1 DMH neurons (n=86 total) had uniform membrane properties

2 DMH neurons expressing the peptide neurotransmitter orex

3 DMH NPY knockdown increased the feeding inhibitory and N

4 DMH was given to male Wistar rats by s.c. injection in a

5 DMH was used to determine the methylation status of >276

6 DMH-11C suppresses acute inflammation in the subcutaneou

7 DMH-lesioned rats also could not develop fever autonomic

8 DMH-NPY neurons expressed Glutamic Acid Decarboxylase (G

9 STAT3 in response to leptin administration, DMH-NPY neurons showed neither.

10 a leptin receptor antagonist failed to alter DMH NPY expression, indicating that leptin may not be th

11 mucosa from control and treated animals and DMH-induced intestinal tumors were assayed for JNK and E

12 In the Arc and DMH, leptin-induced Fos-IR was observed in neurons that

13 increased by 20-65% in the VMH, ARC, ME, and DMH, with no changes elsewhere.

14 o identify the thermoeffector mechanisms and DMH representation of the two phenomena and thus to unde

15 mations of the phenolic hydroxyl (Ph-OH) and DMH side chain for 4 are similar to those of 2.

16 ArcN NPY/AgRP terminal fields in the PVN and DMH decreased SSNA.

17 hat the ArcN NPY neuropathway to the PVN and DMH is pivotal in obesity-induced elevations in SNA.

18 ArcN NPY/AgRP fibers closely appose PVN and DMH presympathetic neurons.

19 ctions from the DLPO and MnPO to the RMR and DMH/DHA emerge from largely separate neuronal population

20 mmon preoptic neurons project to the RMR and DMH/DHA, we injected CTb into the RMR and Fluorogold int

21 sm for crepuscular behavior: if DMH/VLPO and DMH/LHA projections act cooperatively, daily activity is

22 NPY is highly expressed in the lean animal, DMH NPY mRNA expression is observed only after diet-indu

23 hypothalamic nuclei, including the RCA, Arc, DMH, and PMV.

24 We found that CART neurons in the Arc, DMH, and PMV express long form leptin-receptor mRNA, and

25 nl), into the dorsomedial hypothalamic area (DMH) increased BAT SNA by +853 +/- 176 and +898 +/- 249%

26 alamic nucleus and dorsal hypothalamic area (DMH/DHA) with projections to the RMR that may mediate co

27 ast to the consistent expression in the ARH, DMH NPY mRNA expression was undetectable until after 10

28 oci differentially methylated as analysed by DMH between cisplatin sensitive and resistant ovarian ca

29 i between two classes of samples analysed by DMH using CpG island microarrays.

30 e-labeled cells were found within the caudal DMH.

31 To compile DMH maps for the autonomic cold defense and for the cold

32 ng an anxiety-like response in a compromised DMH, and provide the first neuroanatomical basis for lac

33 he conformation of the 1',1'-dimethylheptyl (DMH) side chain, the conformation of the cyclohexyl ring

34 and in vivo activities; the dimethylheptyl (DMH) analog 1e was the most potent in the series.

35 o rat brain membranes of the dimethylheptyl (DMH) analogs increased by an order of magnitude in most

36 ice were treated with 1,2-dimethylhydrazine (DMH), a colon carcinogen.

37 The 1,2-dimethylhydrazine (DMH)-induced colon carcinoma model was used to study the

38 istribution of ACF in the dimethylhydrazine (DMH) model of colonic carcinogenesis in the rat.

39 ee brain regions--lateral (LH), dorsomedial (DMH) and arcuate hypothalamus (ARC)--naltrexone increase

40 the ventral premamillary (PMv), dorsomedial (DMH), and arcuate (ARC) nuclei contained the greatest nu

41 ese neurons is found within the dorsomedial (DMH) and lateral hypothalamus (LH), areas of the brain t

42 e SCN and the LC, including the dorsomedial (DMH) and paraventricular hypothalamic nuclei (PVN), as w

43 the permissive effect of large electrolytic DMH lesions on cold-induced hypothermia was due to suppr

44 y the lack of molecular markers specific for DMH.

45 K activity in hyperproliferative mucosa from DMH-treated animals compared with normal mucosa from con

46 versing BAT SNA activations from POA or from DMH.

47 CCK is released from DMH neurons in response to repeated postsynaptic depolar

48 Furthermore, DMH-NPY projections were not observed within the nucleus

49 Furthermore, DMH/DHA LepRb neurons (and a subpopulation of LepRb mPOA

50 Differential Methylation Hybridisation (DMH) is one technique used for genome-wide DNA methylati

51 d in differential methylation hybridization (DMH) microarray experiments as well as other effects inh

52 lled differential methylation hybridization (DMH), which allows a genome-wide screening of hypermethy

53 r densities in the dorsomedial hypothalamus (DMH) and forebrain regions.

54 tes, including the dorsomedial hypothalamus (DMH) and median preoptic area (mPOA), both critical site

55 lso applied to the dorsomedial hypothalamus (DMH) and retrorubral field (RRF) because such injections

56 the region of the dorsomedial hypothalamus (DMH) appears to generate the sympathetically mediated ta

57 of neurons in the dorsomedial hypothalamus (DMH) appears to play an important role in signaling the

58 ith lesions in the dorsomedial hypothalamus (DMH) challenged with a shock-inducing dose of bacterial

59 excitation in the dorsomedial hypothalamus (DMH) develop panic-like responses, defined as tachycardi

60 es evoked from the dorsomedial hypothalamus (DMH) from which similar cardiovascular changes and incre

61 The dorsomedial hypothalamus (DMH) has been implicated in the coordination of stress r

62 of NPY mRNA in the dorsomedial hypothalamus (DMH) has been observed, suggesting that melanocortin sig

63 The dorsomedial hypothalamus (DMH) has long been implicated in feeding behavior and th

64 amus (ARH) and the dorsomedial hypothalamus (DMH) have been implicated in food intake and obesity.

65 expression in the dorsomedial hypothalamus (DMH) of adult male rats and that this increase in RFRP i

66 DNF in the VMH and dorsomedial hypothalamus (DMH) of adult mice, we were able to elucidate the physio

67 laterally into the dorsomedial hypothalamus (DMH) of mice that express Cre in neurons expressing the

68 expression in the dorsomedial hypothalamus (DMH) of rats with exercise-induced anorexia, implying th

69 of neurons in the dorsomedial hypothalamus (DMH) of the rat by blockade of local GABAA receptors wit

70 ave shown that the dorsomedial hypothalamus (DMH) plays a key role in generating stress-induced physi

71 tic lesions of the dorsomedial hypothalamus (DMH) promoted hypothermia in cold-exposed restrained rat

72 ide Y (NPY) in the dorsomedial hypothalamus (DMH) serves as an important signaling peptide in the reg

73 relays through the dorsomedial hypothalamus (DMH) to LC; this circuit input increases LC activity dur

74 ly stimulating the dorsomedial hypothalamus (DMH), a brain region known to be involved in thermoregul

75 centrations in the dorsomedial hypothalamus (DMH), a midline hypothalamic structure involved in the i

76 inhibition in the dorsomedial hypothalamus (DMH), achieved by chronic microinfusion of the glutamic

77 zone (SPZ) to the dorsomedial hypothalamus (DMH), and thence to ventrolateral preoptic nuclei (VLPO)

78 al circuits in the dorsomedial hypothalamus (DMH), as blocking leptin with a specific antibody, antag

79 ol areas, like the dorsomedial hypothalamus (DMH), require labeling in live tissue slices.

80 In the rat dorsomedial hypothalamus (DMH), serotonin (5-HT) concentrations are altered rapidl

81 nucleus (PVN) and dorsomedial hypothalamus (DMH).

82 cell number in the dorsomedial hypothalamus (DMH).

83 ransmission in the dorsomedial hypothalamus (DMH).

84 ortex (ACC), and dorsal medial hypothalamus (DMH).

85 e dorsomedial and perifornical hypothalamus (DMH/PeF).

86 the dorsomedial nucleus of the hypothalamus (DMH) developed obesity and reduced energy expenditure wi

87 the dorsomedial nucleus of the hypothalamus (DMH) during lactation in the rat is in part due to neura

88 the dorsomedial nucleus of the hypothalamus (DMH) has been implicated in a host of physiological proc

89 The Dorsomedial Nucleus of the Hypothalamus (DMH) is known to play important roles in ingestive behav

90 the dorsomedial nucleus of the hypothalamus (DMH), the arcuate nucleus (Arc), the periventricular nuc

91 the dorsomedial nucleus of the hypothalamus (DMH).

92 novel mechanism for crepuscular behavior: if DMH/VLPO and DMH/LHA projections act cooperatively, dail

93 In DMH-treated animals, long-term treatment with this chemo

94 os (as a surrogate for neuronal activity) in DMH/DHA LepRb neurons, and a large number of mPOA LepRb

95 injections showed robust hrGFP expression in DMH neurons, as visualized by its endogenous fluorescenc

96 carcinomas, lung adenomas, and hepatomas in DMH-treated mice.

97 ate) had increased c-Fos immunoreactivity in DMH neurons expressing the NMDA receptor 1 (NR1) subunit

98 spontaneous post-synaptic currents (PSCs) in DMH neurons, including those projecting to PVN (identifi

99 s of biotinylated dextran amine in the mouse DMH.

100 ular nucleus (PVH), the dorsomedial nucleus (DMH), and the lateral hypothalamic area (LHA), each of w

101 dial nucleus (VMH), the dorsomedial nucleus (DMH), the arcuate nucleus (Arc), and the ventral premamm

102 Both the dorsomedial hypothalamic nucleus (DMH) and the paraventricular hypothalamic nucleus (PVN)

103 the nearby dorsomedial hypothalamic nucleus (DMH) by microinjection of the GABA(A) receptor agonist m

104 icated the dorsomedial hypothalamic nucleus (DMH) in circadian control of sleep, but this hypothesis

105 The dorsomedial hypothalamic nucleus (DMH) is critical for the expression of circadian rhythms

106 ors in the dorsomedial hypothalamic nucleus (DMH) of rats, which is known to elicit cardiovascular an

107 ons in the dorsomedial hypothalamic nucleus (DMH), a region implicated in satiety and stress.

108 cleus, the dorsomedial hypothalamic nucleus (DMH), and the PVH.

109 area, and dorsomedial hypothalamic nucleus (DMH), autonomic regions including the infralimbic cortex

110 Oral administration of DMH-11C reduced the accumulation of pouch fluid leukocyt

111 Third, blockade of DMH NPY1R reversed the sympathoinhibition elicited by se

112 the models are considered in the context of DMH analyses.

113 Next, the effects of DMH-administered Agrp were assessed on intake of two foo

114 populations, fasting decreased expression of DMH NPY expression, while it increased ARH NPY expressio

115 owed us to identify the projection fields of DMH leptin-responsive neurons.

116 To study the function of DMH MC4Rs in energy balance, an MC3/4R-selective agonist

117 ied at 24 weeks after the first injection of DMH.

118 Moreover, we found that knockdown of DMH NPY expression in intact rats reduced NPY content in

119 is to characterize the chemical phenotype of DMH-NPY neurons by comparing the gene expression profile

120 we characterized the efferent projections of DMH-NPY neurons using the anterograde tracer biotinylate

121 le is known about the cellular properties of DMH neurons.

122 depolarized and increased the firing rate of DMH NPY neurons in DIO mice.

123 To further differentiate the regulation of DMH and ARH NPY populations, fasting decreased expressio

124 ted feeding synchronized the daily rhythm of DMH activity in rats such that c-Fos expression in the D

125 yperphagia and obesity, however, the role of DMH-NPY neurons has yet to be characterized.

126 To understand better the role of DMH-NPY neurons, we characterized the efferent projectio

127 hether site-directed chemical stimulation of DMH/PeF neurons evoked changes in IOP, ICP, and the tran

128 Chemical stimulation of DMH/PeF neurons evoked significant increases in HR (+69.

129 Chemical stimulation of DMH/PeF neurons evokes substantial increases in IOP, ICP

130 d by leptin, the effect of this adipokine on DMH NPY neurons is unknown.

131 n presynaptic terminals that then synapse on DMH NPY cells.

132 reover, that its synthesis in the VMH and/or DMH is required for the suppression of appetite.

133 Y receptor Y1 (NPY1R) antagonist into PVN or DMH decreased or increased SSNA, respectively.

134 The PdPN-DMH projection is minimal in gerbils, involving few, if

135 bility of leptin to activate Fos in the PVH, DMH, and LHA appears to be age-dependent and correlates

136 reversed hyperphagia and obesity and reduced DMH NPY levels.

137 ment correlated with the number of remaining DMH neurons, and lesions in cell groups surrounding the

138 ity, confirming the functionality of the SCN-DMH-LC circuit.

139 ithin the DMH, and second, leptin stimulates DMH NPY neurons.

140 future gene-targeted approaches for studying DMH function.

141 that results from Gsalpha mutations and that DMH MC4R/Gsalpha signaling is important for regulation o

142 Importantly, we also demonstrated that DMH NPY neurons coexpress cocaine amphetamine-regulated

143 We also found that DMH orexin neurons project preferentially to LC and expr

144 Together, these results indicate that DMH NPY plays an important role in modulating food intak

145 Therefore, we propose that DMH orexin neurons play a role in modulating the day-nig

146 ic studies of tibiotarsal joints showed that DMH-11C treatment attenuated pannus formation and joint

147 These findings strongly suggest that DMH-NPY neurons could play a distinct role in the contro

148 substituent in the side chain of Delta8-THC-DMH can enhance potency and can also lead to compounds w

149 The DMH contains neurons expressing Neuropeptide Y (NPY) dur

150 The DMH contains orexigenic neuropeptide Y (NPY) neurons, bu

151 es in plasma lactate levels and activate the DMH.

152 Although the DMH neurons project to the rostral raphe pallidus (rRPa)

153 rats were injected via cannula aimed at the DMH with 100 pmol Agrp at 10:00 h and allowed ad libitum

154 Because the DMH projects to the PVN and also contains CRH neurons, w

155 cimol into the intermediate area between the DMH and the PVN attenuated the increases in heart rate b

156 se, but the heavy input to the VLPO from the DMH, which receives direct and indirect SCN inputs, coul

157 ng of BAT thermoregulatory circuits from the DMH/DHA and mPOA.

158 , the present study first showed that in the DMH abundant alpha-MSH and agouti-related protein fibers

159 ne expression profiles of NPY neurons in the DMH and ARH isolated from postnatal NPY-hrGFP mice by mi

160 ) system to alter Npy gene expression in the DMH and examined the effects of these alterations on foo

161 ART neurons in the parvicellular PVH, in the DMH and in the posterior Pe coexpress thyrotropin-releas

162 s stress-induced accumulation of 5-HT in the DMH and suggest that corticosterone may acutely modulate

163 iological and neuroendocrine response in the DMH and that this neuroendocrine response is not due to

164 decreased neuropeptide Y (NPY) levels in the DMH and the paraventricular nucleus (P < 0.05).

165 e activity of LepR-expressing neurons in the DMH caused a rapid reduction of BP in DIO mice, independ

166 sistent with the hypothesis that OCTs in the DMH contribute to the clearance of 5-HT from the extrace

167 sent data suggest that NPY expression in the DMH during chronic hyperphagic conditions plays importan

168 activation of the NPY neuronal system in the DMH during lactation.

169 Thus, disinhibition of neurons in the DMH in conscious rats results in increases in Fos expres

170 stigate the role of neuronal activity in the DMH in the neuroendocrine response to stress.

171 NPY expression in the DMH is low under normal conditions in adult rodents but

172 sults indicate that neuronal activity in the DMH is necessary for the sympathetic and behavioral resp

173 rapid changes in 5-HT concentrations in the DMH is not clear, earlier results suggest that stress-in

174 rapid changes in 5-HT concentrations in the DMH is not clear.

175 ocortin signaling and the induced NPY in the DMH may constitute unique neurocircuitry in mediating en

176 nstrated that the melanocortin system in the DMH not only plays an important role in inducing NPY exp

177 Re-expression of LepRs in the DMH of DIO LepR-deficient mice caused an increase in BP.

178 rtant role in inducing NPY expression in the DMH of lactating rats but also in regulating energy home

179 at AAV-mediated overexpression of NPY in the DMH of lean rats increased food intake and body weight,

180 (CART); however, CART mRNA expression in the DMH peaked earlier in the progression of DIO.

181 the hypothesis that neuronal activity in the DMH plays a role in MDMA-evoked sympathetic and behavior

182 NPY knockdown in the DMH produced a nocturnal and meal size-specific feeding

183 ers had a population of labeled cells in the DMH that was absent, or nearly absent, in subordinates.

184 results show that Gsalpha imprinting in the DMH underlies the parent-of-origin metabolic phenotype t

185 Knockdown of NPY expression in the DMH via AAV-mediated RNA interference ameliorated the hy

186 ty in rats such that c-Fos expression in the DMH was highest at scheduled mealtime.

187 (1) functional GABAB receptors exist in the DMH, and (2) stimulation of these receptors inhibits the

188 Thus, neurons in the DMH, but not in the PVN, play a role in both the cardiov

189 od intake and neuropeptide expression in the DMH, but there is no information on how endogenous CCK a

190 the l/dlPAG require neuronal activity in the DMH, challenging traditional views of the place of the P

191 ed by studies in intact rats, neurons in the DMH, including those projecting to the PVN, are regulate

192 y genes were differentially expressed in the DMH-NPY neurons compared to the ARH.

193 e l/dlPAG depend on neuronal activity in the DMH.

194 ses ipsilateral to the injection site in the DMH.

195 were blocked by cell-specific lesions in the DMH.

196 ved in ependymal and glial-like cells in the DMH.

197 sponses in rats with GABA dysfunction in the DMH.

198 uated suckling-induced NPY expression in the DMH.

199 in the Arc (infundibular nucleus) and in the DMH.

200 by leptin-activated afferents arising in the DMH.

201 serotonin (5-hydroxytryptamine; 5-HT) in the DMH.

202 identified PRV-labeled LepRb neurons in the DMH/DHA and mPOA (and other sites), thus indicating thei

203 nt strong evidence that LepRb neurons in the DMH/DHA and mPOA mediate thermoregulatory leptin action.

204 Specifically, a subpopulation in the DMH/dorsal hypothalamic area (DHA) is stimulated by feve

205 medial hypothalamus (MH), which includes the DMH, with the OCT blocker decynium 22 (D-22) would poten

206 hibitory pathways that tonically inhibit the DMH/DHA and the RMR at baseline, and that hyperthermia r

207 mmature at birth and appear to innervate the DMH, PVH, and LHA in succession, within distinct tempora

208 n of glutamate receptor antagonists into the DMH also suppressed increases in HR and abolished increa

209 respectively), while only injection into the DMH attenuated the accompanying tachycardia (-62%) and p

210 Microinjections of saralasin into the DMH did not block the panic-like responses elicited by i

211 Microinjection of BMI 10 pmol into the DMH in urethane-anesthetized rats resulted in marked tac

212 A-II receptor antagonist saralasin into the DMH of "panic-prone" rats blocked the anxiety-like and p

213 addition, direct injections of A-II into the DMH of these panic-prone rats also elicited panic-like r

214 acer, fluorogold (FG), was injected into the DMH on day 4 postpartum.

215 of the neuronal inhibitor muscimol into the DMH on increases in HR, MAP and T(co) produced by microi

216 lanted with microinjection cannulae into the DMH or the sites anterior to the DMH (i.e., closer to th

217 or muscimol, a neuronal inhibitor, into the DMH prior to intravenous infusion of saline or MDMA in c

218 Microinjecting muscimol into the DMH prior to MDMA prevented increases in core temperatur

219 NMDA, but not non-NMDA, antagonists into the DMH resulted in dose-dependent blockade of the tachycard

220 ts injected with BMI, but not aCSF, into the DMH showed significant increases in heart rate (HR, 110

221 Microinjection of muscimol into the DMH suppressed the heart rate and blood pressure respons

222 thiodide (BMI) 10 pmol (100 nl)(-1) into the DMH was assessed before, and after, injection of the GAB

223 dia evoked by microinjection of BMI into the DMH was mimicked by microinjection of BMI 30 pmol (75 nl

224 uscimol or 100 nl of saline vehicle into the DMH, the PVN, or an intermediate area (including the ros

225 shed by prior injection of muscimol into the DMH.

226 ine or by direct injections of NMDA into the DMH.

227 rochloride (GYKI52466)] antagonists into the DMH.

228 hour) or its inactive isomer D-AG] into the DMH.

229 II (MTII), was injected bilaterally into the DMH.

230 -allylglycine (L-AG) were implanted into the DMH.

231 ted CTb into the RMR and Fluorogold into the DMH/DHA.

232 ) was stereotaxically microinjected into the DMH/PeF region of isoflurane-anesthetized male Sprague-D

233 had high receptor affinities (1-14 nM), the DMH analog 2a being the most potent.

234 We built functional maps of the DMH and found the strongest representation of cold-seeki

235 iate area (including the rostral edge of the DMH and the region between the two nuclei) and were plac

236 on of neuronal activity in the region of the DMH blocks air stress-induced increases in heart rate an

237 examined the effect of disinhibition of the DMH by unilateral microinjection of bicuculline methiodi

238 Lesions of the DMH eliminated these circadian changes in LC activity, c

239 se results establish that the neurons of the DMH have a critical role in the expression of food-entra

240 In the brainstem, disinhibition of the DMH increased Fos expression in the nucleus tractus soli

241 We now show that excitotoxic lesions of the DMH reduce circadian rhythms of wakefulness, feeding, lo

242 Disinhibition of the DMH resulted in dramatic increases in local Fos expressi

243 e A-II type 1 receptors in the region of the DMH was demonstrated using immunohistochemistry.

244 and expands the functional topography of the DMH, a structure that modulates autonomic, endocrine, an

245 ceptors exist in the neural circuitry of the DMH, and that stimulation of these receptors might suppr

246 ll thermal lesions in different parts of the DMH.

247 the tachycardia caused by stimulation of the DMH.

248 pmol/100 nl/side into either the PVN or the DMH prior to air stress reduced the associated increases

249 of saline vehicle into either the PVN or the DMH, air stress resulted in equivalent increases in plas

250 Through these pathways, the DMH may influence a wide range of behavioral circadian r

251 in hypothalamic CART neurons in the PVH, the DMH, the Arc, and the PMV.

252 , and lesions in cell groups surrounding the DMH did not block entrainment by food.

253 hypothermia, we originally thought that the DMH contained a single thermoregulatory site that worked

254 Lesion studies confirmed that the DMH is a substantial relay in this circuit.

255 We also show that the DMH receives both direct and indirect SCN inputs and sen

256 Given that the DMH/PeF neurons may be a key effector pathway for circad

257 ae into the DMH or the sites anterior to the DMH (i.e., closer to the paraventricular nucleus (PVN) o

258 The projections from the ARH to the DMH develop rapidly and are established by the sixth pos

259 nts as well as other effects inherent to the DMH protocol.

260 However, the afferent neuronal input to the DMH that is activated during lactation and is responsibl

261 To determine if POA neurons project to the DMH, we have used nanometer-sized, gold nanoprobes, whic

262 In contrast to the DMH-NPY neurons, NPY expressing neurons have been best c

263 ling activated areas project directly to the DMH.

264 number of mPOA LepRb neurons project to the DMH/DHA.

265 l is pointing away from the C ring while the DMH chain randomly adopts one of four dynamically averag

266 y genes that are highly expressed within the DMH relative to adjacent hypothalamic regions.

267 e coexpressed in the same neurons within the DMH, and second, leptin stimulates DMH NPY neurons.

268 sion of the orexigenic NPY signal within the DMH, and that the chronic hyperleptinemia increases the

269 This DMH-induced tachycardia was markedly suppressed after in

270 Thus, DMH is made up of electrophysiologically similar neurons

271 Thus, DMH-induced tachycardia is mediated through activation o

272 no enhancement in potency for the pentyl to DMH side chain exchange was seen in the mouse vas defere

273 tioned pattern of projections was similar to DMH projections determined by injections of biotinylated

274 he definition of methylation status, it uses DMH data without between-group normalisation and is less

275 he median eminence and in the RCA, Arc, VMH, DMH, and PMV.

276 s involving the VMH, were not altered in VMH/DMH-specific BDNF mutants.

277 Animals with DMH injections showed robust hrGFP expression in DMH neu

278 Male Sprague-Dawley rats were injected with DMH for 24 weeks.

279 Similar findings were observed in mice with DMH-specific deficiency of melanocortin MC4R receptors,

280 ilitate the development of mouse models with DMH-specific genetic manipulations.

281 treated with safflower oil, or treated with DMH-11C in safflower oil.