ライフサイエンスコーパス: DNA barcode

1 fied 265 specimens to species or genus using DNA barcodes.

2 l library containing a highly diverse set of DNA barcodes.

3 fication through the use of species-specific DNA barcodes.

4 roach: high-resolution lineage tracking with DNA barcodes.

5 roducts, whilst still preserving the cognate DNA barcodes.

6 and contig overlap tailored to the needs of DNA barcoding.

7 ent and can introduce serious ambiguity into DNA barcoding.

8 esigned a three-step pipeline for multilocus DNA barcoding.

9 DNA barcoding aids in identification and advances specie

10 We confirmed that the use of DNA barcoding allowed unequivocal identification of this

11 ergent from orthologous mtDNA sequences, and DNA barcoding analysis incorrectly overestimates the num

12 a high-throughput quantitative method using DNA barcodes and a next-generation sequencing technology

13 alytical challenges to lineage tracking with DNA barcodes and discuss its applications to studies of

14 esign-M can also design primers that include DNA barcodes and minimize primer dimerization.

15 t couples multiplex PCR with sample-specific DNA barcodes and next-generation sequencing to enable hi

16 This study used DNA barcoding and 16 S rRNA sequencing as a method to id

17 mediated homology-directed repair (HDR) with DNA barcoding and high-throughput sequencing to simultan

18 ose a potential impediment to application of DNA barcoding and metagenomics for biomonitoring using b

19 n a form that can be analyzed directly using DNA barcoding and pyrosequencing.

20 Therefore, we integrated DNA barcoding and rearing of larvae to identify wood-bor

21 Here we combine microfluidics, DNA barcoding and sequencing to collect chromatin data a

22 velopment and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantit

23 Here, we used a DNA barcoding approach to generate the genotype-to-fitne

24 DNA barcodes are attached to proteins collectively via r

25 t identifications at the species-level using DNA barcodes are feasible, cost-effective, and reliable,

26 boratory and our own used retrovirus-encoded DNA barcodes as unambiguous lineage-tracing tools to add

27 This study demonstrates the utility of DNA barcoding as a tool for regulatory agencies.

28 sed on host identification by morphology and DNA barcoding, as well as the morphology and phylogeneti

29 is study was to compare the performance of a DNA-barcode assay with fatty acid profile analysis to au

30 0 genera and 12 families were analyzed using DNA barcodes based on the ITS2 and psbA-trnH sequences.

31 Here, we study the effect of numts on DNA barcoding based on phylogenetic and barcoding analys

32 that these data will motivate discussions on DNA barcoding based species identification as applied to

33 matK + trnH-psbA is currently applicable for DNA barcoding-based phylogenetic studies on forest commu

34 chain blocking group to enable synthesis of DNA barcoded beads having capture sequences for the cons

35 DNA barcodes can be used to identify cryptic species of

36 We quantified RSD by two DNA barcode combinations (rbcL + matK and rbcL + matK +

37 ve approach involving chemical profiling and DNA barcoding could be applied for authentication of Ice

38 compared with morphological identifications, DNA barcodes (cytochrome oxidase I) revealed significant

39 combination of morphological and multi-locus DNA barcoding diagnoses of diverse arthropod lineages.

40 we add complete nuclear genome sequencing to DNA barcoding, ecological distribution, natural history,

41 eninsula with unprecedented resolution (3502 DNA barcodes for all 228 species), creating a reliable s

42 The database should provide DNA barcodes for data retrieval and similarity search.

43 Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eu

44 The effectiveness of the DNA barcodes for identification (level of discrimination

45 Our integrated approach confirmed novel DNA barcodes for seven species (10 specimens) of woodbor

46 Our database of DNA barcodes for Welsh native flowering plants and conif

47 n one sample, illustrating the usefulness of DNA barcoding for evaluating the safety of honey.

48 egion were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Admi

49 To determine how well DNA barcodes from the chloroplast region perform in fore

50 from each treatment and processed to produce DNA barcodes from the mitochondrial gene cytochrome c ox

51 identification using DNA sequences, known as DNA barcoding has been widely used in many applied field

52 divergence among sister species, traditional DNA barcoding has not been successful for differentiatio

53 Molecular methods, such as DNA barcoding, have the potential to enhance biomonitori

54 composition are based on morphology-based or DNA barcode identification of individuals.

55 DNA barcodes identified 42 species and haplotypes across

56 658 base pairs of the cytochrome c oxidase I DNA barcode in 1,010 specimens from eleven orders of art

57 Here we examined mitochondrial DNA barcodes in a global urban pest, the American cockro

58 present an approach for engineering evolving DNA barcodes in living cells.

59 Deletion strains containing unique DNA barcodes in place of regulator genes were mixed with

60 me b oxidase (COB), have been used to assess DNA barcoding in dinoflagellates, and both failed to amp

61 By incorporating sample-specific DNA barcodes into the amplicons, we analyzed 48 samples

62 In Epi-ID, chromatin status on DNA barcodes is interrogated by chromatin immunoprecipit

63 Recoverability of DNA barcodes is lower using herbarium specimens, compare

64 DNA barcoding is an attractive technology, as it allows

65 chnologies, sample identification (e.g., via DNA barcoding) is still most often done with Sanger sequ

66 The assembly of DNA barcode libraries is particularly relevant within sp

67 uences were then compared to a comprehensive DNA barcode library of the Zingiberales.

68 tions and 101 individuals, built a Tribolium DNA barcode library, and designed species-specific prime

69 DNA barcodes linked to genetic features greatly facilita

70 Using the plant DNA barcode markers rbcL and matK, we have assembled 97.

71 ple parallel amplification primers, multiple DNA barcode markers, 454-pyrosequencing, and Illumina Mi

72 using an Illumina Miseq platform to sequence DNA barcode markers.

73 ide polymorphisms (SNPs) available with core DNA barcoding markers.

74 High-throughput sequencing of environmental DNA barcodes (metabarcoding) offers an alternative to de

75 approach will eclipse existing single-locus DNA barcoding methods as a means to better understand th

76 s to better understand species overlap using DNA barcoding methods suggest coral species are widely d

77 Here we pair rapid DNA barcoding methods with swift assessment of morpholog

78 h-throughput approach, based on the use of a DNA-barcoded mononucleosome library, to profile the bioc

79 is based on the streamlined semisynthesis of DNA-barcoded nucleosome libraries with distinct combinat

80 ly parallel de novo assembly of high quality DNA barcodes of >1400 bp.

81 However, DNA barcoding of cellular proteins remains challenging,

82 By using DNA barcoding of nuclear ribosomal internal transcribed

83 DNA barcoding of tropical insects reared by a massive in

84 DNA barcoding offers an efficient way to determine speci

85 The unique 20 bp DNA 'barcodes' or 'tags' in each deletion strain enable

86 roduce and accumulate diverse mutations in a DNA barcode over multiple rounds of cell division.

87 .5% success rate achieved by the full-length DNA barcode primers.

88 restriction digestion and amplification with DNA barcoding primers.

89 ces proximity records of any nearby pairs of DNA-barcoded probes, at physiological temperature, witho

90 ntified as Saccharomyces bayanus by standard DNA barcoding procedures.

91 DNA barcoding promises to be a useful tool to identify p

92 of diet identifications using a three-locus DNA barcode (rbcL, trnH-psbA and ITS2).

93 the global BOLD Systems database holds core DNA barcodes (rbcL + matK) for about 15% of land plant s

94 Therefore, integrated DNA barcodes, Real-Time PCR assays using TaqMan probes a

95 etto dietary supplements was designed from a DNA barcode reference library created for this purpose.

96 The DNA barcode reference library was developed in BOLD data

97 ragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,00

98 then used to determine the sequence of the "DNA barcoding" region of the cytochrome c oxidase subuni

99 Two DNA barcode regions, namely cytochrome c oxidase subunit

100 Using plant DNA barcoding regions (trnL and rpoC) coupled with High

101 We present the first national DNA barcode resource that covers the native flowering pl

102 To date, no studies have effectively applied DNA barcodes sensu strictu in this manner.

103 We replaced each effector with a specific DNA barcode sequence by allelic exchange and co-infected

104 ate how highly resolved phylogenies based on DNA barcode sequence data will enhance research focused

105 These DNA barcode sequences are also used to reconstruct a rob

106 ur approach recovers a greater proportion of DNA barcode sequences from individuals than does convent

107 ved molecular phylogenies derived from these DNA barcode sequences have the potential to improve inve

108 m global CTFS-ForestGEO network, we analyzed DNA barcoding sequences of 1277 plant species from a wid

109 nuclear proteome, is followed by multiplexed DNA-barcode sequencing.

110 Here, we establish DNA barcoding, species-specific PCR, and real-time PCR t

111 Whereas DNA barcoding strives for rapid and inexpensive generati

112 roup of about 2000 described extant species, DNA barcoding studies have revealed large amounts of unr

113 eed for comprehensive reference databases in DNA barcoding studies, especially when dealing with inva

114 tern Cape and Gauteng) were identified using DNA barcoding, supplemented in certain cases with mitoch

115 In this approach, we generate DNA barcode-tagged AAV libraries and determine a spectru

116 cation (MDA), low-input library preparation, DNA barcoding, targeted capture and next-generation sequ

117 The DNA barcode technique was applied to identify plant food

118 hem to second-generation sequencing, using a DNA barcoding technique that allowed us to quantify inde

119 also rely on genetic analyses including the DNA barcoding technology.

120 We constructed error-correcting DNA barcodes that allow one run of a massively parallel

121 This study was undertaken to generate DNA barcodes that could be utilized in monitoring and cu

122 Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling e

123 ule bisulfite sequencing and sample-specific DNA barcodes to determine the spectrum of MLH1 promoter

124 with the Genera of Fungi project, which adds DNA barcodes to known biodiversity and corrects the appl

125 plex (MHC) multimers labeled with individual DNA barcodes to screen >1,000 peptide specificities in a

126 PLC-QToF-MS chemical profiling together with DNA barcoding to distinguish species and chemotypes of t

127 ose of this study was to test the ability of DNA barcoding to identify the plant origins of processed

128 ged frozen fishery products, this study used DNA barcoding to investigate the breaded hake and plaice

129 limitations with a technology that leverages DNA barcoding to profile chromatin quantitatively and in

130 s issue, we have demonstrated the utility of DNA barcoding to verify the taxonomic identity of fungi

131 ll types in a single culture with the aid of DNA barcodes: unique sequences of DNA introduced to the

132 ersisting cells and identified by sequencing DNA barcodes uniquely labeling each of the tested cytoki

133 e collected species was performed throughout DNA barcoding using ITS, rbcL, rpoC1 and matK sequences.

134 DNA barcoding utilizes species-specific DNA sequence inf

135 Multiamplicon next-generation DNA barcoding was able to recover sequences correspondin

136 d to AAV capsid mutant libraries tagged with DNA barcodes, we can draw a high-resolution map of AAV c

137 Using CRISPR/Cas9 technology and specific DNA barcodes, we devised a strategy to recapitulate and

138 is report, we demonstrate that a three-locus DNA barcode when applied to 296 species of woody trees,

139 tive and practical alternative to exhaustive DNA barcoding when preparing sequencing libraries for hu

140 however, this is especially problematic for DNA barcoding, which attempts to characterize all living