ライフサイエンスコーパス: DNA cleavage

1 ining, RNA degradation, and oligonucleosomal DNA cleavage).

2 increase levels of topoisomerase II-mediated DNA cleavage).

3 ation of recombinants (binding, synapsis and DNA cleavage).

4 o PAM-mediated stimulation of Cas9-catalysed DNA cleavage.

5 A5 was used to assess sites of double-strand DNA cleavage.

6 ormation of normally transient double strand DNA cleavage.

7 may employ a unique catalytic mechanism for DNA cleavage.

8 erse transcription and conduct second-strand DNA cleavage.

9 he iron-sulfur cluster, but had no effect on DNA cleavage.

10 e responsive to targeted HPV genome-specific DNA cleavage.

11 sess a sequence-specific ATPase activity for DNA cleavage.

12 condary to salicylate-mediated inhibition of DNA cleavage.

13 oligonucleotides attached to Rec12 following DNA cleavage.

14 ate metal ions of different atomic radii for DNA cleavage.

15 level of homolog pairing precedes programmed DNA cleavage.

16 shown to facilitate RNA-guided site-specific DNA cleavage.

17 as9 is the limiting factor for Cas9-mediated DNA cleavage.

18 slocation to the nucleus, where it initiates DNA cleavage.

19 ly 360 ns in H(2)O) which is responsible for DNA cleavage.

20 recombination involving transposase-mediated DNA cleavage.

21 ashion primes MjAgo for subsequent rounds of DNA cleavage.

22 turmeric enhanced topoisomerase II-mediated DNA cleavage.

23 et sequence recognition and protein-mediated DNA cleavage.

24 tosine base at the -4 position to facilitate DNA cleavage.

25 acid, and feruloylmethane, had no effect on DNA cleavage.

26 uring long-range communication and following DNA cleavage.

27 hat could otherwise result from RAG-mediated DNA cleavage.

28 this function is separable from its role in DNA cleavage.

29 s can catalyse Z-site-specific double-strand DNA cleavage.

30 ssed SOS induction as a readout of increased DNA cleavage.

31 stood but is associated with enzyme-mediated DNA cleavage.

32 erases has a more stringent requirement than DNA cleavage.

33 laining some ensemble biochemical results on DNA cleavage.

34 increased, likely because of indiscriminate DNA cleavage.

35 e than etoposide at inducing enzyme-mediated DNA cleavage.

36 III, which contains the tyrosine involved in DNA cleavage.

37 e2, and internal protein blocks also trigger DNA cleavage.

38 pable of effecting single- and double-strand DNA cleavage.

39 f FPV resolvase-DNA complexes and subsequent DNA cleavage.

40 metal ion cofactor (Cd(2)(+)) that supports DNA cleavage.

41 valent enzyme-DNA intermediate after initial DNA cleavage.

42 laborate to inhibit the hairpinning stage of DNA cleavage.

43 employ a two-metal ion mechanism to support DNA cleavage.

44 alpha utilizes a two-metal-ion mechanism for DNA cleavage.

45 nosyl homocysteine promotes this promiscuous DNA cleavage.

46 rase II poisons that generate novel sites of DNA cleavage.

47 ble tethering of the nuclease domains during DNA cleavage.

48 exerts a conformational control domain over DNA cleavage.

49 e, the most therapeutically valuable type of DNA cleavage.

50 form a cruciform structure that facilitates DNA cleavage.

51 dation of mutant mtDNA through site-specific DNA cleavage.

52 ide RNAs (gRNAs) to direct sequence-specific DNA cleavage.

53 and is necessary for efficient Ref-mediated DNA cleavage.

54 SPDL motifs position the strand for accurate DNA cleavage.

55 transition mutations without double-stranded DNA cleavage.

56 tional mechanisms must restrict RAG-mediated DNA cleavage.

57 C2c1 depends on both crRNA and tracrRNA for DNA cleavage.

58 functional protein capable of site-specific DNA cleavage.

59 full-length Cas9 and catalyzes site-specific DNA cleavage.

60 died in vitro, where light activation caused DNA cleavage.

61 Here we examine the DNA cleavage activities and substrate requirements of Nm

62 d 6'-deoxy-BLM Z and the evaluation of their DNA cleavage activities as a measurement for their poten

63 n, ferulic acid, and feruloylmethane) on the DNA cleavage activities of human topoisomerase IIalpha a

64 nsistent with loss of transposition specific DNA cleavage activity and acquisition of DNA repair spec

65 mes displayed a marked loss of catalytic and DNA cleavage activity as well as a reduced affinity for

66 NA binding protein Cas9 that have lost their DNA cleavage activity could be used to recruit transcrip

67 DNA cleavage activity does not depend on cleavage of the

68 Purified Sco5333 and Tbis1 displayed weak DNA cleavage activity in the presence of Mg(2+), Mn(2+)

69 is study provides a structural basis for the DNA cleavage activity of 1, will guide the design of syn

70 haride moiety plays an important role in the DNA cleavage activity of BLMs and ZBMs, (ii) the ZBM dis

71 Metnase DNA cleavage activity was not required for Exo1 5'-exonu

72 repeat (TIR) specific DNA-binding activity, DNA cleavage activity, albeit uncoupled from TIR-specifi

73 he latter zinc-binding site is essential for DNA cleavage activity, given that the H937A and H942A mu

74 Moreover, it holds a strong ATP-inhibited DNA cleavage activity.

75 Zn(2+) ions to produce apoprotein abrogated DNA cleavage activity.

76 hese overhangs but did not require Metnase's DNA cleavage activity.

77 s translated into corresponding asymmetry in DNA cleavage activity.

78 of the HNH nuclease domain directly controls DNA cleavage activity.

79 y the monomeric diazofluorene 11 as a potent DNA cleavage agent in tissue culture.

80 guide the design of synthetic DNA-activated DNA cleavage agents, and underscores the utility of natu

81 , are demonstrated to be much less effective DNA cleavage agents, thereby providing an explanation fo

82 DNA cleavage analysis using mutants defective in DNA bin

83 Kinetic DNA cleavage and ATPase measurements implicate R79 in mo

84 e show the bulge and nexus are necessary for DNA cleavage and demonstrate that the nexus and hairpins

85 bination reaction, which can be divided into DNA cleavage and DNA joining steps.

86 resistance to cleavage enzymes may occur if DNA cleavage and error prone repair does not render the

87 ion requires the enzyme to precisely control DNA cleavage and gate opening coupled with ATP hydrolysi

88 that Type III RM enzymes can dissociate upon DNA cleavage and go on to cleave further DNA molecules (

89 cleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the

90 leavage at high drug concentrations, whereas DNA cleavage and inhibition of religation occurs at low

91 ed rotation' of the synaptic complex between DNA cleavage and joining.

92 erminal domain to core-type DNA sites, where DNA cleavage and ligation are executed.

93 plied in host organisms, they enable precise DNA cleavage and ligation without the gain or loss of nu

94 Previous studies have focused on examining DNA cleavage and ligation; however, the dynamic opening

95 leave and package viral DNA, suggesting that DNA cleavage and packaging are inextricably linked.

96 acts with pUL28, but its precise role in the DNA cleavage and packaging reaction is unclear.

97 minase subunit), A374V in UL32 (required for DNA cleavage and packaging), V296I in UL42 (encoding the

98 f seven viral proteins that are required for DNA cleavage and packaging.

99 L)15, pU(L)28, and pU(L)33 with capsids, and DNA cleavage and packaging.

100 yrB subunits, that catalyzes double-stranded DNA cleavage and passage of a second double-stranded DNA

101 uclease targets the submicron locus, causing DNA cleavage and recruiting repair factors such as GFP-5

102 s that must coordinate fuel consumption with DNA cleavage and religation and with numerous conformati

103 e located in the DNA gate domain, where both DNA cleavage and religation take place.

104 exploration of the kinetics of Cas9-mediated DNA cleavage and repair.

105 equired to produce maximal quinolone-induced DNA cleavage and restricted the divalent metal ions that

106 TnpA is in an activated state competent for DNA cleavage and strand transfer.

107 he amino acid was important for DNA bending, DNA cleavage and supercoil relaxation.

108 mice brain by combining CRISPR-Cas9-mediated DNA cleavage and the efficient delivery of donor templat

109 The Cu(GTSCHCl) complex caused distinct DNA cleavage and Topo IIalpha inhibition unlike that for

110 apoptosis confirmed by nuclear condensation, DNA cleavage, and accumulation of S phase cell arrest.

111 isolated and characterized for DNA binding, DNA cleavage, and DNA ligation activities.

112 in each step of transposition: DNA binding, DNA cleavage, and DNA strand transfer.

113 n appears to be topoisomerase II inhibition, DNA cleavage, and free radical generation.

114 and poly(ADP-ribose) polymerase processing, DNA cleavage, and JNK phosphorylation.

115 of RAG1, which contains the active site for DNA cleavage, and RAG2, an accessory factor whose intera

116 arly stage of transpososome assembly, before DNA cleavage, and that mutations affecting immunity have

117 tolerance, which is important for practical DNA cleavage applications.

118 complexes that are blocked at steps prior to DNA cleavage are also described.

119 sts in topoisomerase IIalpha, its effects on DNA cleavage are equivocal.

120 Over 95% of tested sgRNA induced specific DNA cleavage as measured by CEL-1 assays.

121 alpha (top2alpha) as measured by an in vitro DNA cleavage assay and cellular topoisomerase-DNA comple

122 olecules were evaluated in the Top1-mediated DNA cleavage assay and in the National Cancer Institute'

123 eing used as a standard in the Top1-mediated DNA cleavage assay.

124 942A mutants were defective in both in vitro DNA cleavage assays and cellular recombination assays.

125 nt expression in tobacco leaves and in vitro DNA cleavage assays, respectively.

126 een the K86R/G100T and wild-type proteins in DNA cleavage assays.

127 othermophilus (GeoCas9) catalyzes RNA-guided DNA cleavage at elevated temperatures.

128 Free DNA binding suppresses Top1-catalyzed DNA cleavage at high drug concentrations, whereas DNA cl

129 ether and how PAM binding activates Cas9 for DNA cleavage at spatially distant sites.

130 V(D)J recombination entails double-stranded DNA cleavage at the antigen receptor loci by the RAG1/2

131 ce for DNA, enzyme and drug contributions to DNA cleavage at the gate, suggest a mechanism for DNA di

132 to exchange DNA strands after double-strand DNA cleavage at the two recombining att sites, and that

133 te-specific RAG endonuclease, which mediates DNA cleavage at two recombining gene segments and their

134 Using multiphoton absorption and DNA cleavage at unique sites by I-SceI endonuclease, we

135 However, off-target DNA cleavages at unknown sites can lead to mutations tha

136 portant not only for regulating RAG-mediated DNA cleavage but also for the efficiency of RAG recruitm

137 donuclease requiring divalent metal ions for DNA cleavage but not for binding.

138 evidence for looping being a requirement for DNA cleavage, but instead support a diffusive sliding of

139 if resulted in variants that did not promote DNA cleavage, but retained high-affinity DNA binding-thu

140 re recognized as promising intermediates for DNA cleavage, but their formation has thus far been limi

141 ed improvements in the precision of targeted DNA cleavage, but they often restrict the range of targe

142 om 1, provide insights into the mechanism of DNA cleavage by 1.

143 DNA cleavage by 10MD5 proceeds with kobs=2.7 h(-1) and r

144 provide mechanistic insights into RNA-guided DNA cleavage by Cpf1 and establish a framework for ratio

145 Unexpectedly, Mg(2+)-catalyzed DNA cleavage by EcoRI is profoundly inhibited by Cu(2+)

146 DNA cleavage by fCas9 requires association of two fCas9

147 ge reactions; DNA 13 underwent double-strand DNA cleavage by independent single-strand cleavages at a

148 es the inhibitory effect of RAD51 on 3'-flap DNA cleavage by MUS81-EME1 through its RAD51 filament di

149 We show that ATP-dependent DNA cleavage by R-proteins occurs at fixed positions (6-

150 TP/s/monomer) are required for site-specific DNA cleavage by R-proteins.

151 DNA cleavage by RAG occurs only at the G1 phase of the c

152 esidue (K233) results in a large increase of DNA cleavage by RAG1/2.

153 oint to an unusual mechanism of PT-dependent DNA cleavage by restriction enzymes in the face of parti

154 Stimulation of DNA cleavage by SgrAI, at primary as well as secondary s

155 create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells.

156 we found that the target RNA per se induces DNA cleavage by the Cmr complex in vitro.

157 rstanding of the PAM-dependent, crRNA-guided DNA cleavage by the Cpf1 family nucleases.

158 domain of antigen receptors is initiated by DNA cleavage by the RAG1-RAG2 protein complex at sites f

159 DNA cleavage by the Type III restriction enzymes require

160 DNA cleavage by the Type III Restriction-Modification (R

161 te positioned at the active site for optimal DNA cleavage by the tyrosine hydroxyl nucleophile to fac

162 This accelerated site-specific DNA cleavage by the zinc-finger nuclease, without enhanc

163 To examine distinct steps of BER, DNA cleavage by uracil-DNA glycosylase and Ape1 endonucl

164 e corresponding Arg-321 mutation showed that DNA cleavage can still take place in the absence of this

165 es were compared with BLM and deglycoBLM for DNA cleavage, cancer cell uptake, and cytotoxic activity

166 On the basis of time courses of DNA cleavage, cation titrations, and metal ion mixing ex

167 ) affect the binding of the drug to the Top1-DNA cleavage complex and thus modulate the drug's Top1 i

168 ch as camptothecin (CPT), stabilize the Top1-DNA cleavage complex in a DNA sequence-dependent manner.

169 le intercalation into both free DNA and Top1-DNA cleavage complex, whereas larger substituents only a

170 system on the ability to stabilize the Top1-DNA cleavage complex.

171 icancer activity by reversibly trapping Top1-DNA cleavage complexes (Top1cc's) and inducing replicati

172 ulation of pathogenic topoisomerase-1 (Top1)-DNA cleavage complexes (Top1ccs) in murine models of ata

173 erations cause potentially irreversible Top2.DNA cleavage complexes (Top2cc), leading to Top2-linked

174 Topoisomerase IIbeta (Top2beta)-DNA cleavage complexes are known to arrest elongating RN

175 Reversible topoisomerase I (Top1)-DNA cleavage complexes are the key DNA lesion induced by

176 from camptothecin-stabilized topoisomerase I-DNA cleavage complexes in human breast cancer cells.

177 d on camptothecin-stabilized topoisomerase I-DNA cleavage complexes.

178 eptide was detected in human topoisomerase I-DNA cleavage complexes.

179 cterial thiophenes stabilize gyrase-mediated DNA-cleavage complexes in either one DNA strand or both

180 erials that target DNA gyrase by stabilizing DNA-cleavage complexes, but their clinical utility has b

181 I requires K(+) ions to prevent non-specific DNA cleavage, conditions which affect the translocation

182 nt antiproliferative effect of Cas9-mediated DNA cleavage confounds such measurement of genetic depen

183 rmined the 2.9-A-resolution structure of the DNA cleavage core of human topoisomerase IIalpha (TOP2A)

184 ha and -beta occupancy and etoposide-induced DNA cleavage data suggest factors other than local topoi

185 We also observed DNA cleavage determined by TUNEL staining in TBBEC treat

186 We introduce on-chip lysis and non-enzymatic DNA cleavage directly followed by a purifying step for r

187 er pylori (R.HpyAXII) and demonstrated their DNA cleavage, DNA glycosylase and AP lyase activities in

188 meric form of the enzyme is required for its DNA cleavage, DNA-binding, and nonhomologous end joining

189 Sites of topoisomerase I-mediated DNA cleavage do not appear to be affected by supercoil g

190 dem array of zinc fingers, fused to the FokI DNA cleavage domain, to direct double-strand breaks (DSB

191 inal DNA-recognition domain and a C-terminal DNA cleavage domain.

192 DNA-binding modules linked to a nonspecific DNA cleavage domain.

193 embly of zinc finger DNA-binding domain to a DNA-cleavage domain enables the enzyme machinery to targ

194 e highlight the function of RNA in mediating DNA cleavage during genome rearrangements and pathogen d

195 pecific endonuclease that catalyzes specific DNA cleavage during V(D)J recombination, which is requir

196 ed with on- and off-target DNA, we find that DNA cleavage efficiencies scale with the extent to which

197 the presence of mutated phage targets, when DNA cleavage efficiency is reduced.

198 While CRISPR/Cas9 executes double-stranded DNA cleavage efficiently, closure of the broken chromoso

199 the benefits and drawbacks of three types of DNA cleavage enzymes (zinc finger endonucleases, transcr

200 deal scaffolds for engineering site-specific DNA cleavage enzymes for genome editing applications.

201 We are currently engineering DNA cleavage enzymes that specifically target hepatitis

202 leases (meganucleases) are sequence-specific DNA cleavage enzymes used for genome engineering.

203 DNA cleavage enzymes will be delivered as genes within v

204 DNA cleavage enzymes, including homing endonucleases or

205 e the delivery and intracellular activity of DNA cleavage enzymes.

206 ed to amplified loci, the resulting multiple DNA cleavage events can be a cause of false positive hit

207 We propose that DNA cleavage events mediated by RAG endow developing ada

208 pUL33 is necessary for one of the two viral DNA cleavage events required to release individual genom

209 per shows a role for pUL33 in one of the two DNA cleavage events required to release monomeric genome

210 tial rapid endonuclease activity, additional DNA cleavage events then occur more slowly, leading to f

211 inetics of these endonucleases by monitoring DNA cleavage events with deep sequencing.

212 se pair into another without double-stranded DNA cleavage, excess stochastic insertions and deletions

213 lies on guide RNAs that direct site-specific DNA cleavage facilitated by the Cas endonuclease.

214 chromatin condensation, nuclear lamin A and DNA cleavage, fragmentation of the nuclear envelope, and

215 The RAG-2(T490A) mutation uncoupled DNA cleavage from cell cycle and promoted aberrant recom

216 de quinone on topoisomerase IIalpha-mediated DNA cleavage have been examined previously.

217 ne = 1,10-phenanthroline-5,6-dione, leads to DNA cleavage in an oxygen independent manner.

218 off-target analysis to assess Cas9-mediated DNA cleavage in human cells, we demonstrate that "enhanc

219 hile the aglycon (deglycobleomycin) mediates DNA cleavage in much the same fashion as bleomycin, it e

220 e results reveal the role of endonucleolytic DNA cleavage in restriction and yet point to diversity a

221 on that restriction enzymes caused extensive DNA cleavage in the absence of PT modifications in vivo.

222 companied by reduced transient double-strand DNA cleavage in the rDNA-promoter region and reduced pre

223 tyrosine hydroxyl nucleophile to facilitate DNA cleavage in the reaction pathway.

224 sigmoidal in nature, and rates and levels of DNA cleavage increased when metal ion mixtures were used

225 supercoiling, decatenation, DNA binding, and DNA cleavage inhibition assays.

226 Camptothecin-stabilized topoisomerase I-DNA cleavage intermediates in mammalian cells are unique

227 olate modification that renders Sin-mediated DNA cleavage irreversible.

228 The last detectable intermediate prior to DNA cleavage is a low spin Fe(III) peroxy level species,

229 displacement mechanism instead of oxidative DNA cleavage is confirmed by denaturing gel electrophore

230 pH-gated light-activated double-strand (ds) DNA cleavage is controlled by variations in electronic a

231 A design to improve the efficiency of target/DNA cleavage is critical to ensure the success of CRISPR

232 Whereas DNA cleavage is essential for immunity, the function of

233 DNA cleavage is executed by Cas9, which uses two distinc

234 Evidence suggests DNA cleavage is initiated by hydrogen atom abstraction f

235 exhibit reduced chromatin decompaction after DNA cleavage, lesser focal recruitment of homologous rec

236 DNA cleavage mechanism of AgeI is novel among Type IIP r

237 Here, we report studies to evaluate the DNA cleavage mechanism of CglI.

238 Taking advantage of the sequential DNA cleavage mechanism of I-DmoI LAGLIDADG homing endonu

239 a difference in metal ion utilization during DNA cleavage mediated by human topoisomerase IIalpha and

240 om of the scissile phosphate that stimulates DNA cleavage mediated by topoisomerase IIbeta.

241 The type of DNA cleavage might alter the balance between these two a

242 generate a tetramer with two double-stranded DNA cleavage modules.

243 e phosphate greatly enhances enzyme-mediated DNA cleavage, most likely by stabilizing the leaving 3'-

244 e., for strongly bound DNAs, the facility of DNA cleavage must involve other parameters in addition t

245 Double-stranded DNA cleavage of light-activated lysine conjugates is str

246 nt protein that lacks endonuclease activity, DNA cleavage of the 3'-5' strand relative to the wild-ty

247 bp/s at 25 degrees C) and a requirement for DNA cleavage of two recognition sites in an inverted hea

248 biosensors were reported using DNAzymes with DNA cleavage or DNA ligation activity.

249 arse the roles of individual proteins in the DNA cleavage/packaging reaction.

250 g biophysical properties that correlate with DNA cleavage patterns.

251 While having unexceptional DNA cleavage potencies, both glycosylated analogues were

252 We describe an unprecedented DNA-catalyzed DNA cleavage process in which a radical-based reaction p

253 ted with our simulations that Cas9-catalyzed DNA cleavage produces 1-bp staggered ends rather than ge

254 rp pH optimum near 7.5, with greatly reduced DNA cleavage rate and yield when the pH is changed by on

255 duplex are tightly coupled, and the overall DNA cleavage rate is strongly dependent on Sin concentra

256 e find that activating Sin mutations promote DNA cleavage rather than simply stabilize the cleavage p

257 This reversible DNA cleavage reaction is the target of a number of antic

258 critical issue, this study characterized the DNA cleavage reaction of Escherichia coli topoisomerase

259 AG1 and RAG2 proteins catalyze site-specific DNA cleavage reactions in V(D)J recombination, a process

260 bservations, unrelated DNA-catalyzed radical DNA cleavage reactions require redox-active metals and l

261 Key to this capability are targetable DNA cleavage reagents and cellular DNA repair pathways.

262 Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-tar

263 of MtTOP1 was expressed and found to retain DNA cleavage-religation activity and catalyze single-str

264 of the putative active site residues in the DNA cleavage/religation process.

265 DNA cleavage requires a series of protein-DNA complexes

266 of TerL variants for defects in binding and DNA cleavage, revealing that the ATPase domain is the pr

267 casionally leads to autoimmunity due to self-DNA cleavage (self-restriction) [8].

268 7-azaindenoisoquinolines intercalate at the DNA cleavage site in DNA-Top1 covalent complexes with th

269 sulted in several compounds that have unique DNA cleavage site selectivities and potent antitumor act

270 calate between two base pairs outside of the DNA cleavage site, has been suggested to promote deforma

271 y for a cytosine base 4 nt upstream from the DNA cleavage site.

272 equence that is directly centered across the DNA cleavage site.

273 age distinguished 'intrinsic recognition' of DNA cleavage sites by topo IV from drug-induced preferen

274 t redistribute CTCF/cohesin occupancy rewire DNA cleavage sites to novel loop anchors.

275 The DNA cleavage sites were analyzed using ICM Molsoft softw

276 e tetramer and engaged with the enzyme's two DNA-cleavage sites.

277 oth an online archive of LHEs with validated DNA cleavage specificities and DNA-binding interactions,

278 f an extensive set of HE variants with novel DNA cleavage specificities using an integrated experimen

279 However, its pattern of DNA cleavage specificity is different and it is resistan

280 ors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a vers

281 To improve DNA cleavage specificity, we generated fusions of cataly

282 This reaction is initiated through a DNA cleavage step by the RAG1 and RAG2 proteins, which t

283 This DNA cleavage step is followed by a joining step, during

284 up to 11 base pairs in length, which prevent DNA cleavage, still allow formation of a stable complex

285 varying thiophilicities in conjunction with DNA cleavage substrates that substituted a sulfur atom f

286 Kinetic analysis of DNA cleavage suggests flexible tethering of the nuclease

287 reas [Ru(phen)(3)](2+)* does not show direct DNA cleavage, the deprotonated form of 1H(2)O(2+)* does

288 Following DNA cleavage, the HsdR subunits appear unable to dissoci

289 bility because it regulates FEN1's potential DNA cleavage threat near the site of replication.

290 or positioning of the scissile phosphate for DNA cleavage to take place.

291 Programmable DNA cleavage using CRISPR-Cas9 enables efficient, site-s

292 e have also analyzed the metal dependence of DNA cleavage using Mg(2+) ions at different concentratio

293 No DNA cleavage was detected in cells infected with a U(L)1

294 Gel electrophoresis results confirmed that DNA cleavage was not a major inactivating mechanism.

295 In addition, the metal-ion dependence of DNA cleavage was sigmoidal in nature, and rates and leve

296 ablish the role of this sequence in accurate DNA cleavage, we have determined the crystal structure o

297 RVDelta140 genome replication and extent of DNA cleavage were comparable to those for revertant viru

298 We mapped DNA cleavage when a translocating enzyme collides with a

299 AP mediated DNA cleavage within NCPs is initiated by DNA-protein cro

300 hat DNA binding is far more promiscuous than DNA cleavage, yet the molecular cues that govern strand