ライフサイエンスコーパス: DNA endonuclease

1 i-functional maturase protein that is also a DNA endonuclease.

2 ndromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases.

3 break at defined locations by site-specific DNA endonucleases.

4 neering of universal programmable RNA-guided DNA endonucleases.

5 phosphate for bottom-strand cleavage at the DNA endonuclease active site and then reposition the 3'

6 ode reverse transcriptase, RNA maturase, and DNA endonuclease activities for site-specific DNA insert

7 -encoded reverse transcriptase, maturase and DNA endonuclease activities for site-specific insertion

8 that has both RNA maturase and site-specific DNA endonuclease activities in vitro.

9 oded reverse transcriptase and site-specific DNA endonuclease activities required for this process.

10 In eukaryotic cells, a 5' flap DNA endonuclease activity and a ds DNA 5'-exonuclease ac

11 This single-stranded DNA endonuclease activity can act at all single-stranded

12 The DNA endonuclease activity of the enzyme is specific for

13 As for the yeast mtDNA introns, the DNA endonuclease activity of the Lactococcal intron is a

14 We also demonstrate that the single-stranded DNA endonuclease activity of this T. thermophilus domain

15 eA inhibits this mechanism of HGT in cis via DNA endonuclease activity that is localized to the perip

16 vatives displays DNA-binding specificity and DNA endonuclease activity that is similar to that of the

17 oded reverse transcriptase uses a C-terminal DNA endonuclease activity to cleave the opposite strand

18 However, PALF also possesses single-stranded DNA endonuclease activity.

19 ndent endonuclease activity and a non-guided DNA endonuclease activity.

20 he addition of exogenous Zn(2+) inhibits the DNA endonuclease activity.

21 The Rad1-Rad10 complex has a single-stranded DNA endonuclease activity.

22 Interestingly the protein is also a DNA endonuclease, an activity required for intron mobili

23 ossesses manganese-dependent single-stranded DNA endonuclease and 3' to 5' exonuclease activities.

24 purified from yeast, it is a single-stranded DNA endonuclease and a DNA-dependent ATPase, suggesting

25 of the Affymetrix p53 chip and the Surveyor DNA endonuclease and denaturing high-performance liquid

26 I-Ssp6803I represents a novel intron-encoded DNA endonuclease and is the first example of a chromosom

27 eading-frame encoding reverse transcriptase, DNA endonuclease and nucleic acid-binding domains.

28 ngle polypeptide with reverse transcriptase, DNA endonuclease and nucleic acid-binding domains.

29 we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced

30 in and excised intron RNA have site-specific DNA endonuclease and target DNA-primed reverse transcrip

31 , and is a member of the LAGLIDADG family of DNA endonucleases, but appears to have lost DNA cleavage

32 n from Aspergillus nidulans encodes a homing DNA endonuclease called I-AniI which also functions as a

33 , requires sequence-specific single-stranded DNA endonucleases called relaxases or nickases.

34 Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic se

35 eases (LHEs) are a family of highly specific DNA endonucleases capable of recognizing target sequence

36 The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA target

37 nd breaks (DSBs) generated by the RNA-guided DNA endonuclease Cas9 determine how gene function is alt

38 The RNA-guided DNA endonuclease Cas9 has emerged as a powerful tool for

39 ted (Cas) systems employ the dual RNA-guided DNA endonuclease Cas9 to defend against invading phages

40 As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to targe

41 ne systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specifi

42 ng divalent cations in other systems such as DNA-endonuclease complexes and ribozymes.

43 1, redox effector-1), a key redox sensor and DNA endonuclease critical for oxidative DNA damage repai

44 that encode proteins lacking the C-terminal DNA endonuclease domain and for group II intron retrotra

45 ype and mutant LtrA proteins showed that the DNA endonuclease domain contains a single tightly bound

46 A UV-cross-linking assay showed that these DNA endonuclease domain mutations do not block DNA prime

47 Notably, deletion of the DNA endonuclease domain or mutations in its conserved se

48 ified features of the DNA-binding region and DNA endonuclease domain that are conserved in LtrA and r

49 at contributes to DNA binding, followed by a DNA endonuclease domain that contains conserved sequence

50 the intron-encoded protein uses a C-terminal DNA endonuclease domain to cleave the opposite strand an

51 the intron-encoded protein uses a C-terminal DNA endonuclease domain to cleave the opposite strand an

52 licing, while the C-terminal DNA-binding and DNA endonuclease domains are not required.

53 ins have reverse transcriptase, maturase and DNA endonuclease domains characteristic of canonical gro

54 We describe SegF, a novel site-specific DNA endonuclease encoded by gene 69, which is similar to

55 LAGLIDADG and HNH families of site-specific DNA endonucleases encoded by viruses, bacteriophages as

56 Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inte

57 Cas9, an RNA-guided DNA endonuclease found in clustered regularly interspace

58 gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system.

59 PI-SceI, a double-stranded DNA endonuclease from Saccharomyces cerevisiae, is gener

60 Cas9, the RNA-guided DNA endonuclease from the CRISPR-Cas (clustered regularl

61 e GAL1 promoter joined to PvuII, a bacterial DNA endonuclease gene, are toxic to yeast cells even und

62 The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of adva

63 The introns encode the DNA endonucleases I-HmuI and I-HmuII, respectively, whic

64 at this intronic ORF encodes a double-strand DNA endonuclease, I-Ssp6803I.

65 Intron 2 encodes a DNA endonuclease, I-TwoI, with similarity to homing endo

66 reveals the general utility of site-specific DNA endonucleases in producing targeted homologous recom

67 rved sequence motifs characteristic of H-N-H DNA endonucleases, interspersed with two pairs of conser

68 MUS81-EME1 is a DNA endonuclease involved in replication-coupled repair

69 ERCC1-XPF heterodimer, a structure-specific DNA endonuclease, is best known for its function in the

70 trons, the Bastille intron encodes a nicking DNA endonuclease of the H-N-H family, I-BasI, with a cle

71 group J protein (FACJ, BRIP1)-deficient, or DNA endonuclease RBBP8 (CtIP)-compromised cells, whereas

72 we characterized the C-terminal DNA-binding/DNA endonuclease region of the LtrA protein encoded by t

73 Mus81, a DNA endonuclease required for recovery from collapsed fo

74 The DNase was able to digest circular DNA (endonuclease), required both Ca(2+) and Mg(2+) ions

75 independent ICL repair mechanism, in which a DNA endonuclease(s) unhooks an ICL from the leading stra

76 re converted to double-strand breaks using a DNA endonuclease (Surveyor) and oligonucleotide tails ar

77 Human XPF-ERCC1 is a DNA endonuclease that incises a damaged DNA strand on th

78 The td intron of bacteriophage T4 encodes a DNA endonuclease that initiates intron homing to cognate

79 nd Slx4 are subunits of a structure-specific DNA endonuclease that is found in Saccharomyces cerevisi

80 ity) and then with the excised intron form a DNA endonuclease that mediates intron mobility by target

81 associated with the excised intron to form a DNA endonuclease that mediates intron mobility by target

82 ith the excised intron, form a site-specific DNA endonuclease that promotes intron mobility by revers

83 se activity, telomerase is associated with a DNA endonuclease that removes nucleotides from a primer

84 ) the putative HEG encodes a double-stranded DNA endonuclease that specifically cleaves intron-free h

85 Cas9 is an RNA-guided DNA endonuclease that targets foreign DNA for destructio

86 SPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA-DNA complementarity to id

87 Homing endonucleases are sequence-tolerant DNA endonucleases that act as mobile genetic elements.

88 on splicing (maturases) or are site-specific DNA endonucleases that function in intron mobility (a pr

89 member of a new family of structure-specific DNA endonucleases that interact with the replication cla

90 They encode site-specific DNA endonucleases that perpetuate the element within a s

91 t are required for splicing of the intron or DNA endonucleases that promote intron mobility.

92 NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by

93 which employs Surveyor, a mismatch-specific DNA endonuclease, to remove errors from synthetic genes.

94 Schizosaccharomyces pombe ultraviolet DNA endonuclease (UVDE or Uve1p) has been shown to cleav

95 uI and I-BasI are two highly similar nicking DNA endonucleases, which are each encoded by a group I i

96 from Streptococcus pyogenes is an RNA-guided DNA endonuclease with widespread utility for genome modi

97 Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across

98 hila homolog of the human structure-specific DNA endonuclease XPF.