ライフサイエンスコーパス: DNA ladder

1 ic labeling of DNA breaks and formation of a DNA ladder.

2 ated T hybridoma cells, yielding the classic DNA ladder.

3 r DNA of different length, e.g., supercoiled DNA ladder.

4 ng fragments, and separation of the purified DNA ladder.

5 ned by TUNEL (29.8+/-3.2 vs. 3.8+/-0.7%) and DNA ladder.

6 yacrylamide, unlike many currently available DNA ladders.

7 0 bp at a fraction of the cost of commercial DNA ladders.

8 id and can result in the formation of 180 bp DNA ladders.

9 nnexin V positivity, caspase 3 activity, and DNA laddering.

10 ty to <30% after 48 h exposure, and produced DNA laddering.

11 caspases 3 and 8, nuclear fragmentation, and DNA laddering.

12 ted dUTP nick-end labeling index and reduced DNA laddering.

13 dUTP nick end labeling (TUNEL) staining, and DNA laddering.

14 -mediated dUTP nick-end labeling (TUNEL) and DNA laddering.

15 s indicated by a higher degree of fragmented DNA laddering.

16 ed [(32)P]deoxycytidine triphosphate-labeled DNA laddering.

17 ransferase nick end translation (TUNEL), and DNA laddering.

18 and marked decrease of apoptotic bodies and DNA laddering.

19 iotin nick end-labeling (TUNEL) staining and DNA laddering.

20 hours and confirmed by light microscopy and DNA laddering.

21 of FFA oxidation in ZDF islets, both blocked DNA laddering.

22 at can increase the sensitivity of detecting DNA laddering.

23 , including unique morphological changes and DNA laddering.

24 -based resin that improves the resolution of DNA laddering.

25 ine their activities against beta A4-induced DNA laddering.

26 ayed by Hoechst 33258 and TUNEL staining and DNA laddering.

27 poptosis was detected by the TUNEL assay and DNA laddering.

28 Apoptosis of RGC-5 cells was established by DNA laddering.

29 e to apoptotic cell death, as established by DNA laddering.

30 annexin V binding, caspase-3 activation, and DNA laddering.

31 ymerase, caspase-3 activation, and increased DNA laddering.

32 d), and less renal apoptosis, as assessed by DNA laddering.

33 nduced cell death, caspase 3 activation, and DNA laddering.

34 , trypan blue exclusion, and the presence of DNA laddering.

35 mediated dUTP nick-end labeling staining and DNA laddering.

36 (poly(ADP-ribose) polymerase) cleavage, and DNA laddering.

37 irmed by the absence of AIF translocation or DNA laddering.

38 ignificant increase in caspase-3 activity or DNA laddering.

39 , membrane blebbing, caspase activation, and DNA laddering.

40 as assessed by propidium iodide staining and DNA laddering.

41 ages was confirmed by annexin V staining and DNA laddering.

42 ochrome c release, caspase-3 activation, and DNA laddering.

43 h the formation of the apoptotic nucleosomal DNA ladder; 3) drug-induced cleavage within the MLL bcr

44 To assess apoptotic activity, we quantified DNA laddering (32P incorporation), DNA fragmentation (an

45 neurons with internucleosomal DNA cleavage (DNA laddering), a programmed process.

46 ith dilated cardiomyopathy also demonstrated DNA laddering, a characteristic of apoptosis, whereas th

47 ecular events with tissue damage we examined DNA laddering, a common marker of apoptosis.

48 ed in SK-N-AS cells determine the absence of DNA laddering after staurosporine treatment.

49 Apoptosis was identified by DNA ladders after electrophoresis on agarose gels and by

50 DNA laddering (agarose gels) and terminal deoxynucleotid

51 se in myocyte apoptosis and biochemically by DNA laddering (an indicator of apoptosis).

52 Myocardial DNA laddering, an index of apoptosis, reached 20 times t

53 DNA ladder analysis, TUNEL assay, and differential stain

54 n-dUTP nick-end labeling TUNEL assay, and by DNA laddering analysis.

55 Detection of a DNA ladder and in situ labeling (TUNEL) were also consis

56 of apoptosis, which was further confirmed by DNA ladder and terminal deoxyribonucleotide transferase-

57 DNA laddering and activated caspase 3 were also evident.

58 ly inactive C2 dihydroceramide, also induced DNA laddering and BCEC death in a concentration- and tim

59 receptor-mediated toxicity was evaluated by DNA laddering and by quantitative histochemistry [termin

60 hocytes with a pan-caspase inhibitor blocked DNA laddering and caspase-3 activation, but did not bloc

61 minated in apoptotic death, as determined by DNA laddering and caspase-3 cleavage.

62 der galactose control results in nucleosomal DNA laddering and cell death in S. cerevisiae.

63 Kainic acid induced DNA laddering and death of neurons exhibiting a variety

64 sis with TNF-alpha plus DEM was confirmed by DNA laddering and inhibition by caspase inhibitors.

65 ial TG and ceramide and completely prevented DNA laddering and loss of cardiac function.

66 relevant model of neuronal death, apoptotic DNA laddering and morphologic evidence of necrosis can o

67 DNA laddering and nucleosome levels were increased in al

68 l line when treated with I3C, as measured by DNA laddering and poly (ADP-ribose) polymersae (PARP) cl

69 pendently induced apoptosis as documented by DNA laddering and quantified by analysis of cellular chr

70 le cells exhibited apoptosis as evidenced by DNA laddering and quantitated by analysis of nuclear chr

71 of RNA synthesis in MOLT-4 cells within 1 h; DNA laddering and redistribution of phosphatidylserine t

72 imal tubule (LLC-PK1) cells, as evidenced by DNA laddering and TdT-mediated dUTP nick end-labeling as

73 Apoptosis was assessed by DNA laddering and the in situ apoptosis detection assay

74 greater loss of viable myocardium, enhanced DNA laddering and TUNEL, and a greater loss in functiona

75 ase activities, release of cytochrome c, and DNA laddering and was inhibited by caspase inhibitors.

76 marks of apoptosis, such as the formation of DNA ladders and caspase activation, that were observed w

77 inhibition of the L,D-MDP-induced apoptotic DNA ladders and caspase-3 activity in RK(13) cells was o

78 ry, P<0.001) and confirmed by characteristic DNA ladders and TEM findings.

79 f nucleosomal DNA fragments on agarose gels (DNA ladder) and in situ nick end labeling.

80 ine treatment, respectively, and produced no DNA ladder, and cytochrome c remained in the mitochondri

81 vitro, as indicated by cell viability assay, DNA laddering, and Annexin V staining.

82 mination, ultrastructural studies, apoptotic DNA laddering, and antisense techniques, it was shown th

83 ytoplasmic shrinkage, nuclear fragmentation, DNA laddering, and caspase activation.

84 Annexin V staining, DNA laddering, and caspase activity determinations corro

85 apoptosis, including morphological changes, DNA laddering, and caspase-3 activation, whereas piroxic

86 ures of apoptosis, including TUNEL staining, DNA laddering, and caspase-3 activity, all of which were

87 ling (TUNEL) labeling, caspase-3 activation, DNA laddering, and cellular morphology.

88 rase-mediated dUTP-biotin nick end labeling, DNA laddering, and chromatin condensation.

89 his way exhibited diminished cell viability, DNA laddering, and condensed staining of DNA.

90 Higher percentages of apoptotic nuclei, DNA laddering, and cytochrome C release were detected in

91 l and SMC, as determined by TUNEL technique, DNA laddering, and FACS analysis.

92 ion and apoptosis by morphologic assessment, DNA laddering, and flow cytometric analysis of eosinophi

93 ol cells, as assessed by annexin V staining, DNA laddering, and Hoechst 33258 staining.

94 the cleavage of poly(A)DP-ribose polymerase, DNA laddering, and incorporation of fluorescein into the

95 ptosis was confirmed by electron microscopy, DNA laddering, and inhibition by the caspase inhibitor z

96 ic for histone-associated DNA fragmentation, DNA laddering, and TdT-mediated dUTP nick end labeling (

97 Rubisco cleavage, DNA laddering, and victorin binding to the P protein occ

98 , respectively, and displayed characteristic DNA ladders, and cytochrome c was translocated.

99 Autoradiographs of DNA ladders are densitometrically scanned to yield a set

100 l double-strand DNA fragments (also known as DNA ladder) are considered the hallmarks of apoptotic ce

101 H2O2-induced death was associated with DNA laddering as shown by agarose gel electrophoresis.

102 re was prominent endonucleosomic cleavage of DNA (laddering) as well as TUNEL staining.

103 s identified by agarose gel electrophoresis (DNA ladder) as well as in situ nick end labeling.

104 DNA ladder assay, TUNEL assay, and differential staining

105 ptosis as determined by nuclear staining and DNA ladder assay.

106 Neither the DNA laddering assay nor TUNEL staining revealed an incre

107 ollowing serum deprivation, as determined by DNA laddering assay.

108 By using neutral comet and DNA ladder assays, we show that this rapid induction of

109 ferase-mediated UTP-end-labeling (TUNEL) and DNA ladder assays.

110 rminal transferase-mediated end-labeling and DNA laddering assays demonstrated that regression of the

111 200 pg/ml) and IL-1beta (150 pg/ml) produced DNA ladders at 24-72 h.

112 apoptosis was assessed by annexin assay and DNA laddering before and during ACEI therapy.

113 C2-Ceramide without FFA induced DNA laddering, but fumonisin B1, a ceramide synthetase i

114 1beta decreases the adherent cells, produces DNA ladders, but fails to cleave PARP or increase caspas

115 re sensitive than the detection of apoptotic DNA ladder by agarose electrophoresis and is especially

116 The results demonstrate increased DNA laddering by micrococcal nuclease and an increased a

117 stance sensitivity using (a) a finely spaced DNA ladder carrying a restriction site for BamHI, (b) RN

118 dino-2-phenylindole staining, lack of either DNA laddering, caspase-3 activation, or poly(ADP)ribose

119 apoptosis, including chromatin condensation, DNA laddering, cell shrinkage, altered mitochondrial fun

120 anied by the presence of an oligonucleosomal DNA ladder characteristic of apoptosis.

121 timulated myocyte apoptosis, as evidenced by DNA laddering, characteristic nuclear morphology, in sit

122 , chromosome condensation and fragmentation, DNA laddering, cleavage of the death substrate poly(ADP-

123 Man-induced DNA laddering coincided with the activation of a DNase i

124 rks of apoptosis, including the formation of DNA ladders, compaction of nuclear DNA, and the subseque

125 eased apoptosis (caspase 3 fragmentation and DNA laddering) compared with the HSP27 WT mice.

126 (b) DNA fragmentation; (c) the formation of DNA laddering; (d) the cleavage of poly(ADP-ribose) poly

127 hat the apoptotic DNA damage observed in the DNA ladder-deficient SK-N-AS cells is characterized by t

128 Cell cycle analysis and DNA laddering demonstrated a significantly lower number

129 in neuronal death associated with apoptotic DNA laddering despite a necrotic appearance of neurons u

130 ization time-of-flight mass spectrometry for DNA ladder detection was demonstrated.

131 copy for morphology, and electrophoresis for DNA laddering detection, we observed significant apoptot

132 rypan blue exclusion staining, and apoptotic DNA ladder electrophoresis revealed that a subclone deri

133 pan blue staining and deoxyribonucleic acid (DNA) ladder electrophoresis.

134 ptosis and confirmed these observations with DNA laddering experiments.

135 nsferase-mediated dUTP nick end labeling and DNA laddering experiments.

136 lar myocardial tissue showed no demonstrable DNA laddering for any of the groups.

137 Furthermore, DNA ladder formation and PARP cleavage were observed aft

138 Assays for DNA ladder formation and poly-ADP ribose polymerase (PAR

139 ed apoptosis as shown by nuclear morphology, DNA ladder formation, and caspase 3 activation.

140 ncluding cell morphology, annexin V binding, DNA ladder formation, and caspase activation.

141 als and assayed for apoptosis by cell count, DNA ladder formation, and cytochrome c translocation.

142 es in chain length decreased cell viability; DNA ladder formation, Annexin V-FITC two-dimensional flo

143 ed that H2AX phosphorylation is required for DNA ladder formation, but not for the activation of casp

144 ged by poly(ADP-ribose) polymerase cleavage, DNA ladder formation, caspase 3 and 8 activation, and ag

145 Furthermore, DNA ladder formation, CPP32 activation, and PARP cleavag

146 cleavage of poly(ADP-ribose) polymerase, ii) DNA ladder formation, iii) terminal deoxynucleotidyl tra

147 n of CO2/HCO3- as described are inhibited in DNA ladder formation, similar to LR73/hu MDR 1 transfect

148 ted dUTP nick end-labeling technique and for DNA ladder formation.

149 end-labeling staining, a lower incidence of DNA ladder fragmentation, and smaller infarct size.

150 approximately 180 bp and multimers thereof (DNA laddering gel), which are characteristic for DNA fra

151 on (SD)-induced apoptosis as demonstrated by DNA laddering, histone-DNA fragment enzyme-linked immuno

152 free arachidonic acid (AA), as determined by DNA laddering, Hoechst staining, and fluorescein isothio

153 of exogenous human ICAD-L restored apoptotic DNA laddering in ASF/SF2-depleted cells.

154 by TUNEL staining, caspase 3 activation, and DNA laddering in cultured neonatal rat cardiomyocytes.

155 se inhibitor, completely blocked FFA-induced DNA laddering in cultured ZDF islets.

156 ne-pretreated LN-18 cytoplasms do not induce DNA laddering in isolated nuclei from either LN-18 or SH

157 e cytomegalovirus compared with less intense DNA laddering in the posterior segments of eyes of immun

158 in multiples of approximately 180 bp formed DNA ladders in MGCM-treated but not in basal medium-trea

159 hermore, IHNV infection was shown to produce DNA "laddering" in cultured cells.

160 Salt-induced PCD (TUNEL staining and DNA laddering) in primary roots of both Arabidopsis thal

161 mined the occurrence of myocyte apoptosis by DNA laddering, in situ DNA TUNEL labeling, and light and

162 r exhibited significant apoptotic bodies and DNA laddering; in contrast, NRKproHB-EGF were resistant

163 omatin structure, as evidenced by changes in DNA laddering, incorporation of fluorescein into the nic

164 e of poly(ADP-ribose) polymerase cleavage or DNA laddering indicates that the death pathway involved

165 In addition, no evidence of DNA laddering, indicative of cellular apoptosis, was det

166 More intense DNA laddering, indicative of increased apoptosis, was ob

167 hout affecting NF-kappaB activity attenuates DNA laddering induced by DA.

168 tative and qualitative analysis of apoptotic DNA ladders induced by a variety of agents, such as seru

169 d diabetic ZDF islets, apoptosis measured by DNA laddering is increased 3- and >7-fold, respectively,

170 Neither DNA laddering nor the number of apoptotic nuclei were in

171 ibitor ICAD, resulting in the characteristic DNA laddering of apoptosis.

172 Bafilomycin A1 alone caused increased DNA laddering of genomic DNA and increased nuclear stain

173 NE increased DNA laddering on agarose gel electrophoresis and increas

174 The presence of apoptosis was suggested by DNA laddering on electrophoresis in the hemolysate-injec

175 optotic SEC and hepatocytes was supported by DNA laddering on gel electrophoresis and cell morphology

176 this assay were validated by observation of DNA ladders on agarose gels and by morphologic examinati

177 hern blot analysis of nuclear DNA revealed a DNA laddering only in apoptotic cell death.

178 ally and by formation of an oligonucleosomal DNA ladder) only in hepatocytes that had been subjected

179 Hypoxia did not cause DNA laddering or cell loss in cardiac fibroblasts.

180 pases or Bcl-2 degradation and did not cause DNA laddering or cytochrome c release.

181 immunofluorescence coupled with the lack of DNA laddering or other features of apoptosis indicated t

182 ick end labeling of DNA fragments) assay and DNA laddering, or necrosis, assessed by trypan blue excl

183 Neither mycotoxin induced cell death, DNA ladders, or apoptotic bodies in CV-1 cells expressin

184 tic acinar cells, as indicated by histology, DNA laddering, PARP cleavage, and caspase-3 activation.

185 uced apoptosis, as evidenced by induction of DNA laddering, PARP cleavage, and caspase-3/9 activities

186 Cells showed the typical DNA laddering pattern and other characteristics of apopt

187 t does not show the caspase-3 activation and DNA laddering pattern typical of apoptotic cells.

188 Nucleosomal release and a DNA laddering pattern was also observed in the HUVECs in

189 calibration of the separation system with a DNA ladder permits direct estimation of the size of the

190 n of DNA repair systems are critical for the DNA laddering phenotype in apoptosis.

191 , Ac-DEVD-CHO, although the latter abrogated DNA laddering, phosphatidylserine externalization and co

192 While ATA blocked DNA laddering resulting from either beta A4 or okadaic a

193 DNA laddering revealed bands at 200-bp intervals.

194 DNA laddering rose to 19.6% vs. 4.6% in lean control isl

195 (SVPDE), a 3'-exonuclease commonly used for DNA ladder sequencing and as a model enzyme to test nucl

196 leosomal linker, generating oligonucleosomal DNA ladders sharper than those created by micrococcal nu

197 DNA ladder studies also indicated that A-105972 induced

198 nsferase-mediated dUTP nick end-labeling and DNA-laddering studies demonstrated that cardiomyocyte ap

199 poptotic Bcl-x(L), caspase-3 activation, and DNA laddering, suggesting anti-apoptotic activity in oli

200 y, as assessed by infarct area measurements, DNA laddering, terminal deoxynucleotidyltransferase-medi

201 tion and the earlier appearance of apoptotic DNA ladders than at 1700.

202 We show here by TUNEL assay and DNA laddering that DFO induces apoptosis in cultured hum

203 for 24 hours, and apoptosis was assessed by DNA laddering, TUNEL staining, and ELISA for histone-ass

204 layed cell death in which there is prominent DNA laddering, TUNEL-labeling, and nuclei with condensed

205 ation of the characteristic nucleosome-sized DNA ladders, TUNEL-positive staining of cells, activatio

206 Intense light exposure resulted in DNA ladders typical of apoptotic cell death and the simu

207 ype (strain 776) SV40 infection, and a clear DNA ladder was detectable 1 week after infection.

208 Although a DNA ladder was not detected by 12 hours after TNF-alpha

209 DNA laddering was also evident 24 hr after cytokine trea

210 Typical DNA laddering was apparent from 6 to 24 hours at 5 or 10

211 DNA laddering was decreased by mutation of the Tyr(402)

212 A significant amount of DNA laddering was detected 24 hr after ischemia and incr

213 A significant amount of DNA laddering was detected 24 hr after ischemia and was

214 DNA laddering was detected in T3D- but not in mock-infec

215 easured by a fluorescence image scanner, and DNA laddering was evaluated by electrophoresis on 3% aga

216 A significant increase in DNA laddering was evident in both heart and kidney follo

217 DNA laddering was first detected within lesioned tissue

218 DNA laddering was more pronounced after ovariectomy.

219 gnificant activation of executor caspases or DNA laddering was observed, although a dramatic decrease

220 Moreover, DNA laddering was shown in myocytes by agarose gel elect

221 lymerase and generation of nucleosome-length DNA ladders was observed in all cell lines following cis

222 Internucleosomal fragmentation of DNA (DNA ladders) was detected in cells grown with 5 Micron a

223 gments of its own HindIII digest, a standard DNA ladder, was sized by length as well as by fluorescen

224 ic electrophoretic separations of a 400-base DNA ladder were performed in devices fabricated using th

225 ow prevalence of TUNEL-positive myocytes and DNA laddering were detected in 7-week AS mice.

226 Cleavage of poly(ADP-ribose) polymerase and DNA laddering were prevented by preincubation with the c

227 BrdU labelling was decreased, whereas DNA ladders were increased in transgenic animals on day

228 DNA ladders were observed at all the stages, when T+ and

229 DNA ladders were observed in the hearts of all six vehic

230 pical apoptotic features (TUNEL staining and DNA laddering) were seen in rat retinas after 24 hours o

231 viability assay, DNA fragmentation analysis (DNA laddering), Western blot analysis (Anti-poly-(ADP-ri

232 thin 2 h), and they exhibited characteristic DNA laddering when cocultured with HDK-1 T cells in the

233 plasmids which produce both 100 bp and 1 kb DNA ladders when digested with two common restriction en

234 labeled one of the amplification primers, a DNA ladder which is analogous to a "T-sequencing ladder"

235 s (shrunken cells with pyknotic nuclei); (3) DNA laddering which can be blocked by aurintricarboxylic

236 sferase-mediated dUTP nick-end labeling, and DNA laddering, which were associated with inhibition of