ライフサイエンスコーパス: DNA library

1 or forming what has been called a 'satellite DNA library'.

2 or random clones from a small insert genomic DNA library.

3 and used to a probe a P. funiculosum genomic DNA library.

4 nding sequence from a human single-copy (sc) DNA library.

5 nd-read drop-IVT2H method to screen a random DNA library.

6 but one of these classes from a chromosomal DNA library.

7 eta, were isolated from a Paramecium genomic DNA library.

8 eening a recombinant M. tuberculosis genomic DNA library.

9 ruct, screen and finally clone a gene from a DNA library.

10 a1 gene was isolated from a human P1 genomic DNA library.

11 f strain AG84-3, was isolated from a genomic DNA library.

12 oding gene was isolated from a human genomic DNA library.

13 ces in GenBank and from inserts in a genomic DNA library.

14 organs, and used to generate a complementary DNA library.

15 with next-generation sequencing of a genomic DNA library.

16 ed fluorescence compared with the unselected DNA library.

17 using fosmids selected from a total cellular DNA library.

18 ubtracted thymus cDNA library and in genomic DNA libraries.

19 , using it to deplete unwanted sequence from DNA libraries.

20 total DNA instead of cloning fragments from DNA libraries.

21 ement to use global mutagenesis or introduce DNA libraries.

22 able elements to be present in complementary DNA libraries.

23 ly 3 d from flow cytometry to sequence-ready DNA libraries.

24 ired-end sequences derived from large-insert DNA libraries.

25 oxyl radical cleavage data produced from two DNA libraries.

26 tilize nucleotide content changes to produce DNA libraries.

27 From an H. ducreyi 35000HP genomic DNA library, a clone complementing the defect in A77 was

28 s of other groups using the same recombinant DNA library, a comprehensive map was constructed that in

29 We selected from a DNA library an aptamer specifically recognizing human ep

30 -generation sequencing analysis of mate-pair DNA libraries and applied these tools to 16 PTCL patient

31 phage and P1 phage clones from human genomic DNA libraries and characterized by Southern blot and nuc

32 anism to clone biosynthesis genes from large DNA libraries and greatly facilitate the exploration of

33 acterial artificial chromosome mouse genomic DNA library and characterized by polymerase chain reacti

34 se (ADH) was cloned from a Caucasian genomic DNA library and characterized.

35 Also, by using a cosmid DNA library and genomic fractions as hybridization probe

36 Serologic screening of a complementary DNA library and Northern blotting were used to clone the

37 e was transformed with a C. albicans genomic DNA library and screened for the production of active al

38 lysozyme c group were cloned from a genomic DNA library and sequenced.

39 ow-copy sequences were isolated from a human DNA library and tested for their ability to detect restr

40 li strain CS89 was transformed with the same DNA library and then screened for Asp-tRNA(Asp) formatio

41 by sequencing inserts from a CB4856 genomic DNA library and using an informatics pipeline to compare

42 tor of neutrophil elastase into a randomized DNA library and, using the SELEX process, iteratively se

43 isms was identified by screening chromosomal DNA libraries, and cloned.

44 in situ hybridization of chromosome-specific DNA libraries, and mapped >2,000 sites of recombination

45 microfluidics workflow for screening random DNA libraries, and represents a novel alternative method

46 cloning complex individual or combinatorial DNA libraries, and routine or high-throughput cloning of

47 t was used to screen a lambda ZAP II genomic DNA library, and an open reading frame expressing a 28-k

48 DNA libraries are constructed using native genomic DNA f

49 d genes in this region by directly screening DNA libraries as well as by database searching for ESTs.

50 ers, SVCT1 and SVCT2, from rat complementary DNA libraries, as the first step in investigating the im

51 ector ideal for cloning PCR products, making DNA libraries, as well as routine cloning and bacterial

52 d DNA sequencing, hybridization screening of DNA libraries, assembly of gene cassettes, run-off trans

53 .5.1.9) from an Arabidopsis thaliana genomic DNA library based on the homology between the yeast bios

54 rmation in real time and within minutes of a DNA library being loaded.

55 has been cloned from adaptor-ligated genomic DNA libraries by polymerase chain reaction.

56 cloned and used to probe a wild-type Xa23R1 DNA library by colony hybridization.

57 The genes were isolated from a genomic DNA library by complementation of a lipase-deficient tra

58 tein) by screening a HeLa cell complementary DNA library by using the yeast two-hybrid system.

59 e-tag-guided screening of soil environmental DNA libraries can be used to guide the discovery of new

60 Complementary DNA libraries can define the genetic constituents of spe

61 oylphenylalanine, Bpa) amino acids, a single DNA library can be used to incorporate different amino a

62 designed for the propagation of large insert DNA library clones.

63 ed technology that entails construction of a DNA library comprising up to at least 4(7) ( approximate

64 Sequencing of a DNA library constructed from circular plasmid fragments

65 ntaining clones were isolated from a genomic DNA library constructed in our lVET promoter trap vector

66 ms of high molecular weight DNA suitable for DNA library construction and Sanger sequencing.

67 ng ALK translocations, we combined mate-pair DNA library construction, massively parallel ("Next Gene

68 cations in nanotechnology, gene editing, and DNA library construction.

69 Environmental DNA libraries contain large reservoirs of bacterial gene

70 Two cosmids from an E. coli O157:H7 DNA library contain an adherence-conferring chromosomal

71 of IL-6 SOMAmers were isolated from modified DNA libraries containing 40 random positions and either

72 ctivity, we deep-sequenced the products of a DNA library containing 10 random base-pairs on each side

73 A hairpin DNA library containing an 8-base pair sequence-randomize

74 quencing small fragments of a random genomic DNA library containing one or more modular PKS gene clus

75 bidopsis thaliana, and man, (3) large-insert DNA libraries: cosmid, YAC and BAC, and genome physical

76 d in a macroarray format to analyze chimeric DNA libraries created by DNA shuffling.

77 ened over one million clones from metagenome DNA libraries derived from sixteen different environment

78 Complex DNA libraries derived from the host-symbiont population

79 roviral clone, pJSRV21, from a tumor genomic DNA library derived from a natural case of SPA.

80 ce within the equilibrium mixture of masking DNA, library DNA, and target cells.

81 Five cosmids from N. meningitidis DNA library enabled a heme-requiring (hemA), HmbR-expres

82 tion gene homologs found within a large soil DNA library enabled the identification and recovery of a

83 eriments were carried out on circular 103-nt DNA libraries encoding 40-nt randomized sequences as wel

84 a systematic approach in which combinatorial DNA libraries encoding large numbers of random mutations

85 A DNA library encoding potential peptide binders was encap

86 lecules result in the establishment of oligo DNA libraries enriched in DNA molecules containing speci

87 gate amplification mechanisms: sequencing of DNA libraries enriched with tumor-derived palindromic DN

88 ent within GCs, we constructed a recombinant DNA library enriched for cDNAs derived from human genes

89 n 8,000-3,000 years ago by enriching ancient DNA libraries for a target set of almost 400,000 polymor

90 fluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina sequencing.

91 inhibit splicing, we searched human genomic DNA libraries for sequences that would inhibit the inclu

92 harboring members of an F. novicida genomic DNA library, for 4'-phosphatase activity.

93 Subtracted complementary DNA libraries from highly purified murine fetal liver st

94 We compared human complementary DNA libraries from OA-affected and normal cartilage and

95 re, we constructed directional complementary DNA libraries from plaque-periplaque messenger RNA and a

96 A cosmid DNA library from a PAK strain deleted for xcpP-Z was tes

97 pathway in tea, we generated a complementary DNA library from leaf tissue of the blister blight-resis

98 Analysis of single-cell DNA libraries generated by different technologies reveal

99 screening an ordered S. typhimurium genomic DNA library, harbored in Escherichia coli K-12, for expr

100 ectors, with which several very large-insert DNA libraries have been developed.

101 the p79 gene as the bait and a yeast genomic DNA library, identified two independent groups of positi

102 vo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using

103 ies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Ti

104 was isolated by activity screening a genomic DNA library in Streptomyces lividans TK24.

105 colonies of a R. leguminosarum 3841 genomic DNA library in the host strain R. etli CE3.

106 colonies of an R. leguminosarum 3841 genomic DNA library in the host strain S. meliloti 1021.

107 lection and evolution of proteins encoded by DNA libraries, in which individual nascent proteins (phe

108 egy that combines a synthetically engineered DNA library inserted in each run and a new computational

109 We developed quantitative gene assembly and DNA library insertion into the Saccharomyces cerevisiae

110 sed method of highly efficient site-specific DNA library integration.

111 By transferring an F. novicida genomic DNA library into E. coli and selecting for low level pol

112 ology-based screening of large environmental DNA libraries is likely to permit the directed discovery

113 The resulting DNA library is incubated with an affinity-tagged in vitr

114 ast method for introduction of barcodes into DNA libraries made from 5 ng of DNA.

115 hroughput screening of complex environmental DNA libraries more than 40 novel microbial pectate lyase

116 ternative for construction of small-fragment DNA libraries of defined sequences.

117 pA protein, its gene was cloned from genomic DNA libraries of T. denticola.

118 designated chsC and chsG were isolated from DNA libraries of the opportunistic fungal pathogen, Aspe

119 ere selected in vitro from a single-stranded DNA library of 1.8 x 10(15) oligonucleotides showing dis

120 We have designed an effective search of a DNA library of approximately 10(60) in size, and have id

121 st for relapsing fever, we created a genomic DNA library of B. hermsii, screened transformed Escheric

122 A partial genomic DNA library of Chlamydomonas reinhardtii was screened wi

123 A DNA library of F. nucleatum FDC 364 was constructed by p

124 clone these immunogenic antigens, a genomic DNA library of Nine Mile phase I was screened with conva

125 this sequence were used to screen a genomic DNA library of PG31.

126 A DNA library of pRJ28, a large linear plasmid encoding me

127 to the N terminus of the enzyme to screen a DNA library of Pseudomonas sp. MA-1.

128 , arsA and arsB were isolated from a genomic DNA library of S. ovata.

129 erovar Enteritidis, we transformed a genomic DNA library of SE2472 into SE8743.

130 and cobalt ions was isolated from a genomic DNA library of Staphylococcus aureus RN450.

131 Using tpgR1 to probe a genomic DNA library of Streptomyces lividans ZX7, whose linear c

132 For each, a DNA library of V3 region of bacterial 16S ribosomal RNA

133 dCHS2 was isolated by screening a subgenomic DNA library of Wangiella dermatitidis by using a 0.6-kb

134 preserve RNA structure and to limit all RNA-DNA library oligonucleotide interactions to 1:1 stoichio

135 nsformants harboring a Campylobacter genomic DNA library, one recombinant plasmid that encoded a heat

136 t and economical strategies to clone complex DNA libraries or variants of biological modules.

137 Single and multiorganism DNA libraries originating from various environments were

138 omain amplicons derived from saturating soil DNA libraries, our analysis pipeline led to the recovery

139 amplification, and the use of the Nextera XT DNA library preparation kit produced significantly bette

140 atic cleavage-based approach, the Nextera XT DNA library preparation kit, for library preparation.

141 This method can be easily added to existing DNA library preparation techniques and can improve the a

142 In combination with single-stranded DNA library preparation, this method enabled us to recon

143 on data, which are routinely acquired during DNA library preparation, to annotate output sequence dat

144 use WEHI-3 monocytic cell line and a genomic DNA library prepared from 129/SvJ mouse tissue.

145 A genomic DNA library prepared from a benomyl resistant strain of

146 have cloned its gene, gngC, from the genomic DNA library prepared from C. perfringens ATCC10543.

147 , we isolated 14 clones from a complementary DNA library prepared with total RNA extracted from the s

148 Two members of a 10-hairpin DNA library previously found to bind most tightly to the

149 From genomic DNA library production to base-called sequences, this pr

150 While many methods are available for DNA library quantification, there is no unambiguous gold

151 Soil DNA libraries represent large reservoirs of biosynthetic

152 D46 and GTKO porcine aortic EC complementary DNA libraries, respectively.

153 Remarkably, selections employing modified DNA libraries resulted in the first successful isolation

154 genetic information has been 'fished out' of DNA libraries resulting in the discovery of new natural

155 eration sequencing analysis of the assembled DNA library revealed that ligation was accurate, directi

156 Using a complementary DNA library screen, we identified the transcriptional re

157 nesis, mutation detection, DNA modification, DNA library screening and circular DNA production.

158 nesis, mutation detection, DNA modification, DNA library screening and circular DNA production.

159 re we show, using an iterative complementary DNA library screening approach, that human occludin (OCL

160 t the depth-of-coverage yield of single-cell DNA libraries sequenced at arbitrary depths.

161 nterlacing of multiple messages on synthetic DNA libraries showcases the potential of chemical reacti

162 t to screen a yeast two-hybrid complementary DNA library specific for regenerating livers with massiv

163 rotected mRNA fragments are converted into a DNA library suitable for deep sequencing using a strateg

164 titative conversion of the RNA sample into a DNA library suitable for sequencing.

165 the form of adapter-ligated, short-fragment, DNA libraries that may be amplified by polymerase chain

166 tion, we conducted a screen of an M. marinum DNA library that provides 2.6-fold coverage of the entir

167 ing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electro

168 n-for a large randomly generated recombinant DNA library, there exists a staircase of DNA fragments a

169 screen several heterologous and metagenomic DNA libraries, thus enlarging the genomic space that can

170 224,180 Illumina reads from 12 complementary DNA libraries to build what is to our knowledge the firs

171 inaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate

172 Most techniques use PCR to amplify DNA libraries to obtain sufficient quantities for optica

173 gainst standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins an

174 f aptamers have been evolved from an initial DNA library to characterize target cells at the molecula

175 of human PAX6 from single-copy human genomic DNA libraries using cyclic amplification of protein bind

176 with subnanomolar affinity from a randomized DNA library using in vitro selection.

177 The screening of an archived soil DNA library using primers designed to target oxytryptoph

178 High throughput screening of microbial DNA libraries was used to identify alpha-amylases with p

179 An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and r

180 n of the crab MIH and MO-IH genes, a genomic DNA library was constructed from DNA of an individual fe

181 An H. pylori DNA library was cotransformed into SE5000 (pHP8080) and

182 A large-insert domestic cat genomic DNA library was developed using a P1 artificial chromoso

183 ed from a P. radiata (Monterey pine) genomic DNA library was found to possess all of the sequence fea

184 A fosmid DNA library was prepared from a marine picoplankton asse

185 A lambda ZAP II genomic DNA library was screened with the pool of antisera to or

186 A new hairpin DNA library was selected based on affinity for immobiliz

187 OD gene isolated from a normal human genomic DNA library was used to determine the DNA sequence of Mn

188 tinylated RNA baits transcribed from genomic DNA libraries, we are able to capture DNA fragments from

189 functional screen of mammalian complementary DNA libraries, we identified moesin as a novel gene whos

190 ing of a murine choroid plexus complementary DNA library, we identified a new chemokine, designated n

191 From an N. coriiceps testicular DNA library, we isolated a 13.8-kilobase pair genomic cl

192 ter 15 rounds of selection with a structured DNA library, we were able to isolate SRAs that possess l

193 igonucleotide sequences of a full complexity DNA library well below the value for the dissociation co

194 hese painting probes and their corresponding DNA libraries were developed by chromosome micromanipula

195 Genomic DNA libraries were generated from one miniature swine th

196 Woodchuck cDNA and genomic DNA libraries were screened with woodchuck-specific DNA

197 Complementary DNA libraries were sequenced by Solexa.

198 h to interrogate Sox2/Pax6 dimerization on a DNA library where five positions of the Pax6 half-site w

199 ermore, by constructing and screening random DNA libraries with the tet expression shuttle system, 78

200 Probing of a human complementary DNA library with anti-Ma serum resulted in the cloning o

201 uffling method to create a customized random DNA library with flexible sequence design and length.

202 The screening of a genomic DNA library with polyclonal antisera raised against nati

203 nnewicki was isolated by screening a genomic DNA library with serum from a mare that had recently abo

204 liformis was isolated by screening a genomic DNA library with serum from a patient with the chronic v