ライフサイエンスコーパス: DNA ligase

1 ed DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase).

2 s six proteins: UvrA-D, DNA polymerase I and DNA ligase.

3 gth can be modulated by the concentration of DNA ligase.

4 nd the resulting termini are ligated with T4 DNA ligase.

5 n of linear DNA molecules in the presence of DNA ligase.

6 ulation of end-joining in the presence of T4 DNA ligase.

7 uclease 1 to DNA polymerase beta and then to DNA ligase.

8 of a 5' exonuclease, a DNA polymerase and a DNA ligase.

9 incubation times than required when using T4 DNA ligase.

10 ith ligation of these strands mediated by T4 DNA ligase.

11 PO4) and 5'-OH ends that cannot be sealed by DNA ligases.

12 g the mechanism and specificity of mammalian DNA ligases.

13 nd illustrates examples using the Taq and T4 DNA ligases.

14 Therefore, MHEJ and NHEJ require different DNA ligases.

15 l mRNA-capping enzymes and all ATP-dependent DNA ligases.

16 unds was also active against the other human DNA ligases.

17 s, there are three families of ATP-dependent DNA ligases.

18 sis by DNA polymerases or sealing by classic DNA ligases.

19 by DNA exonucleases or ligated by T3 and T4 DNA ligases.

20 DNA ligation reactions used by ATP-dependent DNA ligases.

21 A splint are notoriously poor substrates for DNA ligases.

22 AP) endonuclease 1, DNA polymerase beta, and DNA ligases.

23 Yeast dna2 mutants, like mutants in DNA ligase 1 (cdc9), accumulate low molecular weight, na

24 omosomal DSBs and raise the possibility that DNA ligase 1 (Lig1) may contribute more to A-EJ than pre

25 nt of UHRF1 by the replication machinery via DNA ligase 1 (LIG1).

26 evidence for an unanticipated sufficiency of DNA ligase 1 for these intra-chromosomal events.

27 ow that the tomato (Solanum lycopersicum L.) DNA ligase 1 specifically and efficiently catalyzes circ

28 hamiana Domin plants in which the endogenous DNA ligase 1 was silenced.

29 is study we propose that PSTVd subverts host DNA ligase 1, converting it to an RNA ligase, for the fi

30 A's interaction with Flap endonuclease 1 and DNA Ligase 1, DNA metabolism enzymes.

31 Parp-1, DNA-ligases 1 (Lig1) and 3 (Lig3), and Xrcc1 are implica

32 DNA ligase 3 (Lig3) and its cofactor XRCC1 are widely co

33 ext of multiple genetic knockouts, including DNA ligase 3 and 4 double-knockouts.

34 l extract that contains APE1, pol gamma, and DNA ligase 3.

35 RNA interference-mediated knockdown of DNA ligase 3alpha abolished resistance to apoptotic cell

36 s is associated with increased expression of DNA ligase 3alpha, poly(ADP-ribose) polymerase 1 (PARP1)

37 mplex (Ku) for DSB recognition and the XRCC4/DNA ligase 4 (Lig4) complex for ligation.

38 XRCC4 and DNA Ligase 4 (LIG4) form a tight complex that provides D

39 ne or more requisite C-NHEJ factors, such as DNA ligase 4 (Lig4) or XRCC4, end-joining during CSR occ

40 To compare the specific contribution of DNA ligase 4 (LIG4), Artemis, and DNA-protein kinase cat

41 ing Ku heterodimer, XLF/Cernunnos, and XRCC4/DNA Ligase 4 (Lig4).

42 u70/80 complex for DSB recognition and XRCC4/DNA ligase 4 for ligation.

43 urthermore, mutation of required NHEJ factor DNA Ligase 4 results in enhanced haploid recovery.

44 Surprisingly, we found that DNA ligase 4, essential for NHEJ, did not make a signifi

45 r-chromosomal fusion events in cells lacking DNA ligase 4, in contrast to a remarkably consistent pro

46 Ku or DNA ligase 4-independent alternative end joining (alt-EJ

47 ologous end-joining (NHEJ) factors 53BP1 and DNA ligase 4.

48 Specifically, TDG and DNA ligase activities are reduced by a 3'-flanking 8-oxo

49 4 (LIG4) form a tight complex that provides DNA ligase activity for classical non-homologous end joi

50 duction in the rate of pol beta synthesis or DNA ligase activity on any of the fragments bound to GR-

51 e I whereas LigIII is the only mitochondrial DNA ligase and is essential for the survival of cells de

52 es expressing BCR-ABL1 to the combination of DNA ligase and PARP inhibitors correlates with the stead

53 on of these cell lines with a combination of DNA ligase and PARP inhibitors inhibited ALT NHEJ and se

54 However, T4 DNA ligase and RtcA can use 3'-phosphorylated nicks in d

55 nuclear gene encodes the only mitochondrial DNA ligase and so is essential for this organelle.

56 n strategy which expliots the specificity of DNA ligase and the speed of isothermal amplification to

57 plications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

58 Lig, is a conserved feature of ATP-dependent DNA ligases and GTP-dependent mRNA capping enzymes.

59 profiling of the substrate specificities of DNA ligases and illustrates examples using the Taq and T

60 fferent nucleotide content parallels that of DNA ligase, and optimal ligation efficiency is attained

61 omotes DNA end-joining in the presence of T4 DNA ligase, and this property is mediated by the C-termi

62 tidyltransferase superfamily of RNA ligases, DNA ligases, and RNA capping enzymes.

63 ajor DNA polymerases (Pol I and Pol III) and DNA ligase are directly involved with oligo recombinatio

64 rase B (PolB), flap endonuclease (Fen1), and DNA ligase are required to complete ribonucleotide proce

65 DNA ligases are essential guardians of genome integrity

66 DNA ligases are essential guardians of genomic integrity

67 jacent to the catalytic region of eukaryotic DNA ligases are involved in specific protein-protein int

68 DNA ligases are required for DNA replication, repair, an

69 f base excision repair can be mediated by T4 DNA ligase as well as human DNA ligase I or ligase IIIal

70 nd an OB domain (these two are common to all DNA ligases) as well as a distinctive beta-hairpin latch

71 he ligation fidelity of Thermus thermophilus DNA ligase at a range of temperatures, buffer pH and mon

72 ction instead of the existing chemical or T4 DNA ligase-based methods allows quantitative conversion

73 Whereas DNA ligase-based RASL assays suffer from extremely low a

74 Globular domains from both the human DNA ligase binding protein XRCC4 and bacteriophage varph

75 Crystal structures of DNA ligases bound to nucleotide and nucleic acid substra

76 ized plasmids, indicating that an additional DNA ligase can support NHEJ.

77 ed DNA structures with abnormal DNA termini, DNA ligase catalytic activity can generate and/or exacer

78 RNA and DNA ligases catalyze the formation of a phosphodiester b

79 We apply DNA ligase-catalyzed cyclization kinetics experiments to

80 The development and in-depth analysis of T4 DNA ligase-catalyzed DNA templated oligonucleotide polym

81 We describe the application of T4 DNA ligase-catalyzed DNA templated oligonucleotide polym

82 We have developed a method for the T4 DNA ligase-catalyzed DNA-templated polymerization of 5'-

83 T4 DNA ligase catalyzes phosphodiester bond formation betwe

84 Chlorella virus DNA ligase (ChVLig) has pluripotent biological activity

85 Chlorella virus DNA ligase (ChVLig) is a minimized eukaryal ATP-dependen

86 Chlorella virus DNA ligase (ChVLig) is an instructive model for mechanis

87 nformational dynamics of the Chlorella virus DNA ligase (ChVLig), a minimized eukaryal ATP-dependent

88 The DNA ligase D (LigD) 3'-phosphoesterase (PE) module is a

89 e homodimeric DNA end-binding protein Ku and DNA ligase D (LigD), a modular enzyme composed of a C-te

90 ogous end-joining (NHEJ) catalysed by Ku and DNA ligase D (LigD).

91 e strand break (DSB) repair driven by Ku and DNA ligase D (LigD).

92 eplication in the absence of the replicative DNA ligase, DNA ligase I.

93 The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to syn

94 of the enzyme helps coordinate the entry of DNA ligase during Okazaki fragment maturation.

95 Escherichia coli DNA ligase (EcoLigA) repairs 3'-OH/5'-PO4 nicks in duple

96 Paradoxically, when DNA ligases encounter nicked DNA structures with abnorma

97 Eukaryotes possess multiple DNA ligase enzymes, each having distinct roles in cellul

98 ts to generate cells devoid of mitochondrial DNA ligase failed.

99 RNA ligase 1; Rnl1) and the NAD(+)-dependent DNA ligase family (Escherichia coli LigA), captured as t

100 for mechanistic studies of the ATP-dependent DNA ligase family.

101 Next, DNA repair activities of DNA ligase, flap endonuclease and RNase H2 were monitore

102 trand phosphates at the outer margins of the DNA ligase footprint; (ii) essential contacts of Ser-41,

103 stereochemical preferences of AP endo and T4 DNA ligase for phosphorothioate substrates, we show that

104 notion that DNA ligase III (LIG3), the only DNA ligase found in mitochondria, is essential for viabi

105 DNA ligases have broad application in molecular biology,

106 Human DNA ligase I (hLigI) joins Okazaki fragments during DNA

107 Human DNA ligase I (hLigI) participates in DNA replication and

108 were applied to identify inhibitors of human DNA ligase I (hLigI).

109 DNA ligase I (LIG1) catalyzes the ligation of single-str

110 (Pol delta), flap endonuclease 1 (FEN1) and DNA ligase I (Lig1).

111 (pol beta), flap endonuclease 1 (FEN1), and DNA ligase I (LigI).

112 Members of the DNA ligase I and IV families are found in all eukaryotes

113 or that stabilized complex formation between DNA ligase I and nicked DNA.

114 idues reduced the in vitro ubiquitylation of DNA ligase I by Cul4-DDB1 and DCAF7.

115 Based on the crystal structure of human DNA ligase I complexed with nicked DNA, computer-aided d

116 om human fibroblasts harboring a mutation in DNA ligase I displayed reduced MHEJ activity.

117 Because DNA ligase I has been reported to be ubiquitylated, we u

118 l redundancy between DNA ligase IIIalpha and DNA ligase I in excision repair.

119 nockdown of DCAF7 reduced the degradation of DNA ligase I in response to inhibition of proliferation

120 lication factor C, DNA polymerase delta, and DNA ligase I in the absence of DNA via its non-conserved

121 biquitylated lysine residues and showed that DNA ligase I interacts with and is targeted for ubiquity

122 azaki fragments by the flap endonuclease and DNA ligase I joins nascent fragments.

123 14 nuclear extracts with an antibody against DNA ligase I or III also significantly reduced MHEJ.

124 found that siRNA mediated down-regulation of DNA ligase I or ligase III in human HTD114 cells led to

125 e mediated by T4 DNA ligase as well as human DNA ligase I or ligase IIIalpha-XRCC1 complex.

126 the enzymes flap endonuclease 1 (FEN-1) and DNA ligase I that complete the processing and joining of

127 k left after flap removal could be sealed by DNA ligase I to complete fragment joining.

128 Furthermore, APE1 stimulated DNA ligase I to resolve a long double-flap intermediate,

129 ta was used to measure repair synthesis, and DNA ligase I was used to seal the nick.

130 cleus, LigIII has functional redundancy with DNA ligase I whereas LigIII is the only mitochondrial DN

131 L82 inhibited DNA ligase I, L67 inhibited DNA ligases I and III, and L

132 ding pocket within the DNA-binding domain of DNA ligase I, thereby inhibiting DNA joining.

133 ropriate relative stoichiometry of FEN-1 and DNA ligase I, which compete for binding to proliferating

134 h to map ubiquitylation sites and screen for DNA ligase I-associated E3 ubiquitin ligases.

135 n the absence of the replicative DNA ligase, DNA ligase I.

136 PCNA clamp, its loader RFC, and completed by DNA ligase I.

137 roduction of nicks that could be sealed with DNA ligase I.

138 idates specifically inhibited purified human DNA ligase I.

139 These data indicate that DNA ligases I and III are required in MHEJ.

140 L82 inhibited DNA ligase I, L67 inhibited DNA ligases I and III, and L189 inhibited DNA ligases I,

141 a cell-free assay to determine the roles of DNA ligases I, III and IV in MHEJ and NHEJ.

142 ed DNA ligases I and III, and L189 inhibited DNA ligases I, III, and IV in DNA joining assays with pu

143 nce of polynucleotide kinase (PNK) and human DNA ligase III (Lig III).

144 le lines of evidence support the notion that DNA ligase III (LIG3), the only DNA ligase found in mito

145 Mammalian DNA ligase III (LigIII) functions in both nuclear and mi

146 ymerase-1, X-ray cross-complementing factor1-DNA ligase III and enzymes involved in processing 3'-blo

147 pears to occupy the same binding site as the DNA ligase III catalytic core.

148 Human DNA ligase III contains an N-terminal zinc finger domain

149 amilies are found in all eukaryotes, whereas DNA ligase III family members are restricted to vertebra

150 Human DNA ligase III has essential functions in nuclear and mi

151 separate, independent DNA-binding modules in DNA ligase III that each bind specifically to nicked DNA

152 ed for Iduna ubiquitination of PARP1, XRCC1, DNA ligase III, and KU70.

153 on with other experiments, demonstrated that DNA ligase III, but not ligase IV or ligase I, is primar

154 g PAR polymerase-1, 2 (PARP1, 2), nucleolin, DNA ligase III, KU70, KU86, XRCC1, and histones.

155 demonstrated high levels of PARylated Chd1L, DNA ligase III, SSrp1, Xrcc-6/Ku70, and Parp2 in pluripo

156 ere discovered, including PARP-1, hMutSbeta, DNA ligase III, XRCC1, and PNK.

157 g affinity and specificity of each domain of DNA ligase III.

158 Elevated levels of DNA ligase IIIalpha (LigIIIalpha) have been identified a

159 with a FLT3 inhibitor demonstrate decreased DNA ligase IIIalpha and a reduction in DNA deletions, su

160 is significant functional redundancy between DNA ligase IIIalpha and DNA ligase I in excision repair.

161 Expression of DNA ligase IIIalpha and the association between MRN and

162 ame is true for a protein complex comprising DNA ligase IIIalpha and the scaffolding protein X-ray re

163 DNA ligase IIIalpha and WRN form a complex that is recru

164 n alternative NHEJ repair pathway, involving DNA ligase IIIalpha and WRN.

165 rmed that the expression levels of PARP1 and DNA ligase IIIalpha correlated with the sensitivity to t

166 ike its other nuclear functions, the role of DNA ligase IIIalpha in alternative NHEJ is independent o

167 DNA ligase IIIalpha is a component of an alternative non

168 In addition, DNA ligase IIIalpha is essential for DNA replication in

169 DNA ligase IIIalpha is frequently overexpressed in cance

170 therapy to inhibit FLT3/ITD signaling and/or DNA ligase IIIalpha may lead to repair that reduces repa

171 Furthermore, "knockdown" of either DNA ligase IIIalpha or WRN leads to increased accumulati

172 fold protein XRCC1, DNA polymerase beta, and DNA ligase IIIalpha play pivotal roles in BER.

173 Thus, the expression levels of PARP1 and DNA ligase IIIalpha serve as biomarkers to identify a su

174 Concomitantly, levels of DNA ligase IIIalpha, a component of ALT NHEJ, are increa

175 es with the steady state levels of PARP1 and DNA ligase IIIalpha, and ALT NHEJ activity.

176 d DNA ligase IV, are down-regulated, whereas DNA ligase IIIalpha, and the protein deleted in Werner s

177 , poly-(ADP-ribose) polymerase 1 (PARP1) and DNA ligase IIIalpha, were increased in the BCR-ABL1-posi

178 ermore, the interaction between PNKP and the DNA ligase IIIalpha-XRCC1 complex significantly increase

179 ular apurinic/apyrimidinic endonuclease, and DNA ligase IIIalpha-XRCC1, performs uracil-initiated bas

180 In contrast, DNA ligase IIIalpha-XRCC1, which completes BER, was appr

181 IIIalpha and the association between MRN and DNA ligase IIIalpha/XRCC1 are altered in cell lines defe

182 MRN interacts with DNA ligase IIIalpha/XRCC1, stimulating intermolecular li

183 en two factors, hMre11/hRad50/Nbs1 (MRN) and DNA ligase IIIalpha/XRCC1, that have been linked with al

184 ge T4 RNA ligase 2, as well as against human DNA ligase IIIbeta, indicated a considerable degree of s

185 We find that LIG4, a DNA ligase in DNA double-strand break repair, is a direc

186 system to show increased sensitivity over T4 DNA ligase in the specific detection of a target mRNA.

187 DNA was dependent on expression of the viral DNA ligase, in accord with previous proteomic studies.

188 Here, we show that, although the DNA ligase inhibitor selectively targets mitochondria, c

189 Thus, these novel human DNA ligase inhibitors will not only provide insights int

190 Linking together of DNA strands by DNA ligases is essential for DNA replication and repair.

191 Although a shared feature of DNA ligases is their envelopment of the nicked duplex as

192 rmine the specific cellular functions of the DNA ligase isozymes.

193 r (Yku70-Yku80), MRX (Mre11-Rad50-Xrs2), and DNA ligase IV (Dnl4-Lif1), as well as the ligase-associa

194 cs), XRCC4-like factor (XLF), and XRCC4 (X4)-DNA ligase IV (L4).

195 The XRCC4 (X4)-DNA Ligase IV (LIG4) complex (X4LIG4) executes the final

196 DNA ligase IV (LIG4) is an essential component of the no

197 logous end-joining (NHEJ) DNA repair protein DNA ligase IV (LIG4) lead to immunodeficiency with varyi

198 nduced apoptosis after ionizing radiation or DNA ligase IV (Lig4) loss in the Mre11(ATLD1/ATLD1) nerv

199 Deletion of DNA ligase IV (Lig4), a core component of the NHEJ pathw

200 ical NHEJ (c-NHEJ) components, which include DNA ligase IV (LIG4), and instead arise from alternative

201 reviously, we showed that mice deficient for DNA ligase IV (Lig4), another key NHEJ factor, succumbed

202 ns, including KU70, KU80, ARTEMIS, DNA-PKcs, DNA ligase IV (LIG4), Ataxia telangiectasia mutated (ATM

203 double-strand break repair (DSBR) proteins, DNA Ligase IV (Lig4), Xrcc2, and Brca2, or combined Lig4

204 ) which relies on Ku binding to DNA ends and DNA Ligase IV (Lig4)-mediated ligation.

205 By somatic gene targeting of DNA ligase IV (Lig4; a key component of NHEJ) in a B cel

206 seven genes Ku70, Ku86, DNA-PK(cs), Artemis, DNA Ligase IV (LIGIV), X-ray cross-complementing group 4

207 of nucleases, DNA polymerases, and the XRCC4-DNA ligase IV (X4-LIV) complex in an order influenced by

208 a model in which XLF, by in situ recharging DNA ligase IV after the first ligation event, promotes d

209 nce of some end joining on only Ku and XRCC4.DNA ligase IV allows us to formulate a physical model th

210 sociates with the core NHEJ components XRCC4-DNA ligase IV and Ku.

211 have evolved mechanisms based on the loss of DNA ligase IV and perhaps other unknown molecules to dis

212 subunit (DNA-PKcs), together with the XRCC4-DNA ligase IV complex (X4L4), are major factors.

213 XLF stimulates the XRCC4/DNA ligase IV complex by an unknown mechanism.

214 te that APLF promotes the retention of XRCC4/DNA ligase IV complex in chromatin, suggesting that PARP

215 se mu and lambda to add nucleotides; and the DNA ligase IV complex to ligate the ends with the additi

216 Surprisingly, Mre11 and DNA ligase IV degradation do not appear to be significan

217 protein kinase catalytic subunit, and XRCC4-DNA ligase IV do not modulate PALF nuclease activity on

218 the precise visualization of XRCC4, XLF, and DNA ligase IV filaments adjacent to DSBs, which bridge t

219 structurally related proteins important for DNA Ligase IV function.

220 as well as the association of XLF with XRCC4.DNA ligase IV in vivo suggested a role in the final liga

221 1, B1, D, and B2, respectively) only affects DNA ligase IV levels.

222 e-molecule FRET analysis of the Ku/XRCC4/XLF/DNA ligase IV NHEJ ligation complex, that end-to-end syn

223 s2-Lif1 interactions abrogated both NHEJ and DNA ligase IV recruitment to a DSB.

224 3' overhanging nucleotides and permit XRCC4-DNA ligase IV to complete the joining process in a manne

225 XRCC4 forms a tight complex with DNA Ligase IV while XLF interacts directly with XRCC4.

226 t disruption of DSB repair factors (Rad51 or DNA ligase IV) or DSB sensing factors (ATRIP or MDC1) re

227 In flies deficient in lig4 (DNA ligase IV), a component of the major NHEJ pathway, t

228 complexes: Yku70-Yku80 (Ku), Dnl4-Lif1-Nej1 (DNA ligase IV), and Mre11-Rad50-Xrs2 (MRX).

229 mote HDR at the expense of NHEJ, we targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using t

230 espectively) cause the degradation of Mre11, DNA ligase IV, and p53, infection with Ad3, Ad7, Ad9, an

231 se by facilitating the degradation of Mre11, DNA ligase IV, and p53.

232 ed end joining assay that includes Ku, XRCC4-DNA ligase IV, and PALF, PALF is able to resect 3' overh

233 athway that operates in the absence of XRCC4/DNA ligase IV, and that the requirement for PARP-3 and A

234 teins in the major NHEJ pathway, Artemis and DNA ligase IV, are down-regulated, whereas DNA ligase II

235 by RNA interference diminished expression of DNA ligase IV, Artemis, and Ku80 components in DNA-depen

236 largely resemble those from patients lacking DNA ligase IV, Artemis, or ATM, suggesting that these fa

237 We found that insertions required Ku80 and DNA ligase IV, as well as polymerase IV.

238 s been known that Ku-dependent NHEJ requires DNA ligase IV, it is unclear which DNA ligase(s) is requ

239 DNA ligase IV, on the contrary, is not required in MHEJ

240 recipients deficient in the NHEJ component, DNA ligase IV, the majority of products arise by HR with

241 induced the association of these factors in DNA ligase IV-deficient cells.

242 B) repair that is more active when the major DNA ligase IV-dependent pathway is defective.

243 w that cohered sister telomeres are fused by DNA ligase IV-mediated nonhomologous end joining.

244 XLF-Cernunnos (XLF) is a component of the DNA ligase IV-XRCC4 (LX) complex, which functions during

245 ze as heterotypic filaments independently of DNA Ligase IV.

246 J is circumvented by overexpression of XRCC4/DNA ligase IV.

247 n, as well as targeting the effector protein DNA ligase IV.

248 epair complex, the tumor suppressor p53, and DNA ligase IV.

249 Thr181 to trigger its dissociation from the DNA ligase IV/XRCC4 complex, and promotes its interactio

250 cs autophosphorylation, and it also inhibits DNA ligase IV/XRCC4-mediated end rejoining.

251 entral to the molecular mechanism of NHEJ is DNA ligase IV/XRCC4/XLF complex, which rejoins the DNA.

252 plex of NHEJ factors that includes a ligase (DNA Ligase IV; L4) that relies on juxtaposition of 3 hyd

253 autonomous enzymatic modules: ATP-dependent DNA ligase (LIG), DNA/RNA polymerase (POL), and 3' phosp

254 cribe a complete NHEJ complex, consisting of DNA ligase (Lig), polymerase (Pol), phosphoesterase (PE)

255 Among the mammalian genes encoding DNA ligases (LIG), the LIG3 gene is unique in that it en

256 d by deletion of either of the two remaining DNA ligases (Lig1 and nuclear Lig3) in Lig4(-/-) cells.

257 NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria, where the

258 NAD(+)-dependent DNA ligases (LigAs) are ubiquitous in bacteria and essen

259 r in mammalian cells involves three distinct DNA ligases: ligase I (Lig1), ligase III (Lig3) and liga

260 Nevertheless, PBCV-1 DNA ligase ligated all sequences tested with 10-fold les

261 PBCV-1 DNA ligase ligated ssDNA splinted by RNA with kcat appro

262 um smegmatis that requires the ATP-dependent DNA ligase LigC1 and the POL domain of LigD.

263 nd binding protein Ku and the polyfunctional DNA ligase LigD.

264 ning (NHEJ) system that includes a dedicated DNA ligase (LigD) and the DNA end-binding protein Ku.

265 ing system of DNA repair driven by dedicated DNA ligases (LigD and LigC).

266 i that are critical for ligation by the NHEJ DNA ligase, LigIV.

267 ssential step in most repair pathways is the DNA ligase-mediated rejoining of single- and double-stra

268 out a synthetic lethal screen with cdc9-p, a DNA ligase mutation with two substitutions (F43A/F44A) i

269 es the Vsr endonuclease, DNA polymerase I, a DNA ligase, MutS, and MutL to function at peak efficienc

270 Bacterial DNA ligases, NAD(+)-dependent enzymes, are distinct from

271 ndicating that the rate-limiting step for T4 DNA ligase nick sealing is not a chemical step but rathe

272 SplintR Ligase is 100X faster than either T4 DNA Ligase or T4 RNA Ligase 2 for RNA splinted DNA ligat

273 3'-PO4 ends that cannot be sealed by classic DNA ligases or extended by DNA polymerases.

274 ation of RNA-splinted DNA by Chlorella virus DNA ligase (PBCV-1 DNA ligase).

275 3 gene is unique in that it encodes multiple DNA ligase polypeptides with different cellular function

276 y classic ATP-dependent and NAD(+)-dependent DNA ligases, prevents template-independent nucleotide ad

277 nM at 25 degrees C under conditions where T4 DNA ligase produced only 5'-adenylylated DNA with a 20-f

278 n of Ku80 and a molecular mechanism by which DNA ligase proficient complexes are assembled during NHE

279 protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged

280 requires DNA ligase IV, it is unclear which DNA ligase(s) is required for Ku-independent MHEJ.

281 DNA ligases seal 5'-PO4 and 3'-OH polynucleotide ends vi

282 ment synthesis that was cleaved by Fen1, and DNA ligase sealed the nick for complete repair.

283 tection method that utilizes Chlorella virus DNA ligase (SplintR((R)) Ligase).

284 findings led to a series of experiments with DNA ligase that reveal, contrary to expectation based up

285 e mitochondria or expressing Chlorella virus DNA ligase, the minimal eukaryal nick-sealing enzyme, or

286 Unlike reactions that use T4 DNA ligase, this protocol does not require synthesis of

287 city of a DNA-dependent RNA polymerase and a DNA ligase to act as RNA-dependent RNA polymerase and RN

288 (Fen1) and the resultant nick was ligated by DNA ligase to form a mature lagging strand.

289 ely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerization o

290 bearing deletions or mutations in Ku or the DNA ligases to interrogate the contributions of LigD's t

291 ng a DNA polymerase, an RNA polymerase and a DNA ligase, to use Fe2+ in place of Mg2+ as a cofactor d

292 utants of KU70, KU80, and the plant-specific DNA Ligase VI (LIG6) showed increased stable transformat

293 Here, we report the characterization of DNA LIGASE VI, which is only found in plant species.

294 red in their specificity for the three human DNA ligases were analyzed further.

295 with the DNA-binding domain of Ligase IV, a DNA Ligase which plays essential roles in DNA repair and

296 igation strategy based on USER Enzyme and T4 DNA ligase, which allows the simultaneous and seamless a

297 Mammalian cells have three ATP-dependent DNA ligases, which are required for DNA replication and

298 Humans have three genes encoding DNA ligases with conserved structural features and activ

299 he cyclase pathway resemble those of RNA and DNA ligases, with the key distinction being that ligases

300 t the modification site that is sealed by T4-DNA ligase, yielding a product strand missing the modifi