ライフサイエンスコーパス: DNA melting

1 ransient clamp closure as a prerequisite for DNA melting.

2 ter recognition and contributes to localized DNA melting.

3 tching force is interpreted as force-induced DNA melting.

4 quences closely associated with the start of DNA melting.

5 on of entropy and heat-capacity changes upon DNA melting.

6 ssible role for such interaction in promoter DNA melting.

7 ucture representing an early conformation in DNA melting.

8 main is involved in promoter recognition and DNA melting.

9 key role for this base in the nucleation of DNA melting.

10 ering solution conditions to favor transient DNA melting.

11 articipates in both promoter recognition and DNA melting.

12 the most reliable quantitative indicators of DNA melting.

13 uctural changes in the promoter DNA, such as DNA melting.

14 at G-quadruplex formation is correlated with DNA melting.

15 r sequencing but not with fluorescence based DNA melting.

16 romoter contacts and plays a crucial role in DNA melting.

17 for helicase activity and also origin (ori) DNA melting.

18 re exerted at a step preceding nucleation of DNA melting.

19 ons for two aromatic amino acids involved in DNA melting.

20 on the thermodynamics and kinetics of duplex DNA melting.

21 of a functional, multimeric E1 helicase and DNA melting.

22 rase (RNAP), direct promoter DNA binding and DNA melting.

23 eron attributable to DNA bending rather than DNA melting.

24 GMP alone can greatly increase the level of DNA-melting.

25 icant heat capacity increase associated with DNA melting, all of the above assumptions are self-consi

26 DNA melting analysis holds great promise for simple and

27 man single base variants examined by spatial DNA melting analysis included rs354439, HTR2A 102T > C,

28 Fluorescent high-resolution DNA melting analysis is a robust method of genotyping an

29 nd demonstrate a highly specific intracavity DNA melting analysis scheme utilizing an optofluidic las

30 is paper, we first theoretically investigate DNA melting analysis using an optofluidic laser and then

31 the three warfarin-related sites by spatial DNA melting analysis with 100% accuracy.

32 uidic device was used to demonstrate spatial DNA melting analysis with the resolution and reproducibi

33 enotyping of PCR products by high-resolution DNA melting analysis.

34 recognition of the oxdC promoter, stimulate DNA melting and activate transcription by core RNA polym

35 This remodeling facilitates DNA melting and allows the transition to the open comple

36 re scanned in buffer containing 1 mM Mg(2+), DNA melting and capsid denaturation both contribute to t

37 d stability of Tus-Ter interaction caused by DNA melting and capture of a flipped base by Tus generat

38 to perform biochemical reactions, including DNA melting and detection of single base mismatches.

39 The results are discussed in the context of DNA melting and flexibility around the TATA box region a

40 E1 has origin recognition and ATP-dependent DNA melting and helicase activities, and it consists of

41 strate that separate functional elements for DNA melting and helicase activity can be distinguished.

42 l properties of nanoscale molecular systems (DNA melting and nanoscale water meniscus formation), the

43 f RNAP parts normally associated with stable DNA melting and open complex formation.

44 We examined DNA melting and promoter clearance by using potassium pe

45 red by ATP binding and hydrolysis for origin DNA melting and replication fork unwinding.

46 flexible RNAP clamp domain, concomitant with DNA melting and template loading.

47 The mutation exhibits no defects in DNA melting and translocation in vitro but confers a mod

48 e describe has implication for understanding DNA melting and unwinding reactions, which are generally

49 rrect NTPs suggested that it may represent a DNA melting and/or base pairing step.

50 abilises the preinitiation complex, enhances DNA melting, and stimulates abortive and productive tran

51 a coli RNA polymerase, deficient in promoter DNA melting, and variants of the P(R) promoter of bacter

52 Details of the reaction coordinate for DNA melting are fundamental to much of biology and biote

53 to downstream fork junctions is coupled with DNA melting around the transcription start point.

54 We consider studies of DNA melting as a function of ionic strength and show tha

55 r distortion or base flipping could initiate DNA melting as the second step in DNA replication.

56 he newly developed assay incorporated FO-SPR DNA melting assay, previously developed by our group.

57 fic promoter recognition but cannot initiate DNA melting at the -10 promoter element.

58 lex; and (iii) promote or maintain localized DNA melting at the upstream edge of the bubble.

59 e experimental evidence for the formation of DNA melting bubbles driven by high tension and prove the

60 does not prevent nucleation of the promoter DNA melting but instead blocks its propagation towards t

61 helicase and polymerase both participate in DNA melting, but each enzyme melts the junction base pai

62 nd the recognition of promoter sequences and DNA melting by holoenzyme, transcription initiation and

63 xidation was used to probe for the extent of DNA melting by human immunodeficiency virus, type 1 (HIV

64 n and reveal a novel interaction involved in DNA melting by RNA polymerase.

65 ed on which we suggest that Mtf1 facilitates DNA melting by trapping the non-template strand in the u

66 NAP, likely providing the mechanism by which DNA melting can occur in a minimal factor configuration,

67 ications of the pathogens are carried out by DNA melting curve analysis or gel electrophoresis.

68 is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR prod

69 DNA melting curves at different concentrations of WP631

70 DNA melting curves obtained from each sample were also a

71 e the use of LASR to measure single-molecule DNA melting curves with approximately 1 degrees C accura

72 binding affinity has been investigated using DNA melting (DeltaT(M)), circular dichroism (CD), and su

73 complex stability and region 1.2 involved in DNA melting during initiation.

74 ces between surface immobilized and solution DNA melting dynamics, which allows us to better understa

75 T. aquaticus RNA polymerase impair promoter DNA melting equally at temperatures from 25 to 75 degree

76 greater net dehydration of these bases upon DNA melting; ethylene glycol local accumulation is pract

77 gp2.5 lowers the phage lambda DNA melting force as measured by single molecule force s

78 By studying the rate-dependent DNA melting force in the presence of gp2.5 and its delet

79 switch to (i) allow propagation of nucleated DNA melting from an upstream DNA fork junction and (ii)

80 the -10 region; and (iii) the propagation of DNA melting from the nucleation region is not rate-limit

81 unterpart, provides promoter recognition and DNA melting functions to the holoenzyme.

82 micking promoter DNA where the nucleation of DNA melting had taken place.

83 transcription initiation complex where full DNA melting has not yet occurred.

84 ircular dichroism spectroscopy revealed that DNA melting in the -4/-3 cross-link was greatly inhibite

85 otions control the promoter search and drive DNA melting in the absence of external energy sources.

86 ns of WP631 were fitted to McGhee's model of DNA melting in the presence of ligands, yielding an inde

87 Differential thermal analysis of DNA melting in these assays allowed analytical discrimin

88 capacity increase DeltaC(p) associated with DNA melting, in the range of 40-100 cal/mol K per base p

89 g a mechanism for the nucleation of promoter DNA melting initiation in which RNA polymerase destabili

90 eotide-dependent temporal pathway leading to DNA melting involving a small set of sigma54-DNA conform

91 We propose that DNA melting is an active process initiated in RPc and th

92 The amount or extent of DNA melting is not significantly affected by the length

93 a(54)-RNAP forms promoter complexes in which DNA melting is only triggered by an activator and ATP hy

94 (2) DNA melting is temperature-dependent, with a tm between

95 ation complex, which occurs concomitant with DNA melting, is coordinated with an opening of the RNAP

96 Our results indicate that target DNA melting may be a crucial step during RAG-mediated tr

97 fluorescence probes in conserved region 2.3 (DNA "melting motif") was prepared by replacing tryptopha

98 hich core enzyme mediates the final stage of DNA melting near the transcription start site, and that

99 al dehybridization, this optically triggered DNA melting occurs at a solution temperature that is 22

100 atively agree with a model that asserts that DNA melting occurs during the overstretching transition.

101 tween RNA polymerase and a promoter involves DNA melting of approximately 14 base pairs.

102 ver, some biochemical evidence suggests that DNA melting of multiple base pairs may occur separately

103 molecules bind to the duplex, which induces DNA melting of the duplex remote from the lesion.

104 ce of the next correct dNTP, indicating that DNA melting only occurs after the formation of the terna

105 We demonstrate that prior to DNA melting, only the sigma(54)-factor directly interact

106 In vitro, His-pi.F107S-dependent local DNA melting (open complex formation) occurs in the absen

107 DNA at temperatures well below the onset of DNA melting or capsid denaturation.

108 ld be located in a DNA contact important for DNA melting or is associated with activator interaction

109 ent RNA polymerase-promoter complex involves DNA melting over a region of about 12 base-pairs, which

110 range interactions, the length dependence of DNA melting parameters per base pair, the applicability

111 s can be considered the initial steps of the DNA melting pathway.

112 ence in support of models which describe the DNA melting process accompanying open complex formation

113 The promoter DNA melting process is subject to activation by an enhan

114 Comparing the DNA melting profiles in reference plant materials and in

115 However, the precise role of DNA melting remains unknown.

116 We propose that DNA melting requires the cooperation of the E1 helicase

117 We show that DNA melting requires the simultaneous presence of Rpo41

118 e stretching is used to lower the barrier to DNA melting, resulting in direct mechanical manipulation

119 mplexes serves as a nucleation point for the DNA melting seen in open promoter complexes and restrict

120 absence of Mg(2+) and lower ionic strength), DNA melting shifts to lower temperatures and the two eve

121 ce of large scale lipid phase transitions or DNA melting, small temperature-dependent changes in the

122 he nucleotide-binding domain and induces the DNA melting so that the substrate DNA can access Mre11.

123 al promoters, where regulation occurs at the DNA melting step.

124 roofreading of DNA by epsilon subunit is the DNA-melting step.

125 DNA melting studies using a homogeneous 214 bp DNA fragm

126 s supported by the Na(+)-dependent nature of DNA melting studies, in which significantly higher Na(+)

127 tent with nearest-neighbor data derived from DNA melting studies.

128 arding several fundamental assumptions about DNA melting, such as the absence of longer range interac

129 es, in particular those that depend on local DNA melting, such as the initiation of replication and t

130 ismatches should be implemented in models of DNA melting, such as the widely used thermodynamic neare

131 versed by addition of ethidium bromide or by DNA melting, suggesting that flavopiridol binds to (and

132 Permanganate assays for DNA melting support this view.

133 th the approximately 6 degrees C decrease in DNA melting temperature of the modified oligonucleotide,

134 ese data and from a well-known dependence of DNA melting temperature on G.C content, the contribution

135 pH, f(ov)(pH), from the known dependence of DNA melting temperature on pH.

136 significantly below the regular (zero force) DNA melting temperature, the overstretching force, f(ov)

137 At temperatures slightly above the regular DNA melting temperature, we predict stabilization of dsD

138 linkers are uniformly distributed above the DNA melting temperature, while visibly accumulating at t

139 vable from nanorod-based complexes below the DNA melting temperature.

140 ambient solution temperature well below the DNA melting temperature.

141 perature that is 22 degrees C lower than the DNA melting temperature.

142 e titration and effects of ligand binding on DNA melting temperatures, concluding that isothermal tit

143 .36 M urea and 19.2 % formamide to lower the DNA melting temperatures.

144 Finally, we define a standard state for DNA melting, the temperature at which thermal contributi

145 ow the controls over protein recruitment and DNA melting to be separated, enhancing the diversity of

146 ad defects that appear to be associated with DNA melting to create the fork junction.

147 most distal upstream contacts accompanied by DNA melting to form open complexes.

148 ently linking both DNA strands, ICLs prevent DNA melting, transcription, and replication.

149 The DNA melting transition temperature was found to vary lin

150 hed data on DeltaH and DeltaS values for the DNA melting transition under various conditions.

151 quantitatively translated into multiplexable DNA melting transitions within 30 min.

152 ermination of protein/DNA binding, localized DNA melting transitions, and mRNA production at physiolo

153 es sigma(54) regulatory sequences needed for DNA melting upon activation.

154 just downstream from -10 hexamer to prevent DNA melting upon RNAP binding were used to mimic RNAP-pr

155 The signal of the DNA melting was enhanced by means of gold nanoparticle l

156 rature data for effects of urea and of GB on DNA melting, we propose that urea is an effective nonspe

157 f the mutations on the mechanism of promoter DNA melting were investigated by studying the interactio

158 We further infer that (i) the nucleation of DNA melting, which occurs during the isomerization from

159 a theoretical analysis of the probability of DNA melting within the plasmid as a function of superhel