ライフサイエンスコーパス: DNA nick

1 a putative mimetic of the 5'-phosphate at a DNA nick.

2 flap DNA substrate and processing it into a DNA nick.

3 nicked DNA-adenylate, but not to a standard DNA nick.

4 inantly ligase-adenylate, binds tightly to a DNA nick.

5 denylate but has low affinity for a standard DNA nick.

6 ave a feeble capacity to seal 3'-OH/5'-PO(4) DNA nicks.

7 lex RNA or an RNA:DNA hybrid but cannot seal DNA nicks.

8 DNA hybrid, but it cannot seal 3'-OH/5'-PO4 DNA nicks.

9 ctive nucleotide incorporation indicative of DNA nicking.

10 ion-induced acidosis, loss of viability, and DNA nicking.

11 cally in apoptotic cells, around the time of DNA nicking.

12 inhibiting religation and to a lesser extent DNA nicking.

13 ndensation and collapse, and single-stranded DNA nicking.

14 ath pathway characterized by single-stranded DNA nicking.

15 viability assessed by morphology and reduced DNA nicking.

16 The DNA nicking activity of the wild-type enzyme is still ac

17 Furthermore, we show that the DNA nicking activity of TOP1 is a prerequisite for robus

18 tail or head-to-tail repeat only supported a DNA nicking activity.

19 ge agent, displaying significant supercoiled DNA-nicking activity at concentrations as low as 1 micro

20 Both LigD proteins seal DNA nicks, albeit inefficiently.

21 The cloning and expression of the CviPII DNA nicking and modification system encoded by chlorella

22 ed magnetic tweezers to directly measure the DNA nicking and religation activities of RepC, the repli

23 esidues (Y18,19,26,27) that are required for DNA nicking and religation were displaced up to 14 A out

24 onserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate

25 y arise from DNA regions expected to undergo DNA nicking and/or double-strand breaks.

26 Metnase possesses a unique DNA nicking and/or endonuclease activity that mediates c

27 l change within the nucleosome to facilitate DNA nicking and/or fragmentation.

28 Exo1 processing at DNA nicks and double-strand breaks creates long stretche

29 ath pathway characterized by single-stranded DNA nicks and other features of apoptosis.

30 SR, and we propose that MMR proteins convert DNA nicks and point mutations into dsDNA breaks for both

31 e findings, purified CdtB(H154A) lacked both DNA-nicking and cell cycle arrest activities.

32 we developed two biochemical methods; i.e., DNA-nicking and DNA-gyrase methods to examine whether ce

33 positioning is unaffected by the presence of DNA nicks, and can occur on closed-circular DNAs in the

34 med during the cleavage reaction: open ends, DNA nicks, and hairpin ends.

35 to discrete nucleosomal fragments, although DNA nicks are readily discernible by terminal deoxynucle

36 DNA nicks are the most common form of DNA damage, and if

37 The appearance of DNA nicked at both signals is stimulated by more than an

38 d us to study the consequences of inflicting DNA nicks at EcoRI sites in vivo.

39 on the opposite strand from the Top1-induced DNA nicks at ribonucleotide sites.

40 se activity of topoisomerase I (Top1) causes DNA nicks bearing 2',3'-cyclic phosphates at ribonucleot

41 Hyper-rec mutants, in which DNA nicks become detectable, are synthetic-lethal with r

42 e catalytic core of ligase III is the second DNA nick-binding module.

43 s in single-strand rather than double-strand DNA nicks/breaks.

44 g of 5'-phosphate and 3'-hydroxyl termini at DNA nicks by means of a series of three nucleotidyl tran

45 rvations suggest that an increased number of DNA nicks can overwhelm the repair capacity of DNA ligas

46 e, a PleI isoschizomer, does not exhibit any DNA nicking/cleavage activity, being completely blocked

47 In addition to its classic DNA nicking-closing functions, Top1 plays critical noncl

48 mediates, including single-nucleotide-gapped DNA, nicked DNA, and nicked DNA with various lengths of

49 riety of substrates including partial duplex DNA, nicked DNA, forked DNA structures, blunt duplex DNA

50 atalyses four phosphoryl transfer reactions (DNA nicking, DNA hairpin formation, hairpin resolution a

51 nt, high-affinity binding to single-stranded DNA, nicked double-stranded DNA, and RNA.

52 s suggests that, although DNA ligase I seals DNA nicks during replication, repair, and recombination,

53 Apoptotic cell death was characterized by DNA nick end and single-stranded DNA labeling.

54 d apoptosis by terminal transferase-mediated DNA nick end labeling assay and measured expression of a

55 rminal deoxynucleotidyl transferase-mediated DNA nick end-labeling-positive neurons contained p18 or

56 TNF-alpha-induced apoptosis was detected by DNA nick-end labeling and by measuring histone associate

57 own by a decrease in cell viability, in situ DNA nick-end labeling, and internucleosomal DNA fragment

58 had a marked apoptotic effect documented by DNA nick-end labeling, or DNA agarose gels in xenografts

59 By DNA nick-end labeling, the number of dying putaminal cel

60 aluated 20-22 h after sepsis by annexin V or DNA nick-end labeling.

61 t had been appropriately preserved to permit DNA nick-end labeling.

62 ronchoalveolar lavage (BAL), histopathology, DNA nick-end-labeling assays, and electron microscopy.

63 is includes: (1) labeling of cell nuclei for DNA nicked ends; (2) morphological changes in ultrastruc

64 Gamma is a strand-specific and site-specific DNA nicking enzyme (YCG downward arrowGT or AC upward ar

65 dependent cleavage of molecular beacons by a DNA nicking enzyme, through which one target DNA can ope

66 e large subunits behave as sequence-specific DNA nicking enzymes and only nick the bottom strand of t

67 These DNA-nicking enzymes could prove useful for investigation

68 4 bp downstream from the Top1 site enhances DNA nicking especially when the 3' end of the TFO is pro

69 out in which peroxynitrite-mediated plasmid DNA nick formation in the presence or absence of organos

70 nt complex formation, but not resealing of a DNA nick in a preformed covalent complex.

71 ains of Cas9, to create a guide RNA-directed DNA nick in the context of an in vitro-assembled CRISPR-

72 s observation, coupled with the detection of DNA nicking in cells subjected to metabolic inhibition,

73 ed only GzmA to reconstitute single-stranded DNA nicking in isolated nuclei.

74 ivation and SET cleavage at N175, triggering DNA nicking in wild-type, but not AEP null, mice.

75 t the ligase IIIalpha-XRCC1 complex binds to DNA nicks in nucleosomes only when they are exposed by p

76 sulting in the conversion of a proportion of DNA nicks into DNA lesions that require recombination fo

77 DNA nick sensor, but it is not required for DNA nick joining activity in vitro.

78 nc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet th

79 ained in part by a shift toward a processive DNA nicking mechanism, which leads to a higher frequency

80 DNA nicking occurred independently of caspase activation

81 io are demonstrated via the marked effect of DNA nicking on histone eviction that underscores the pow

82 repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-d

83 The target of APTX is 5'-adenylates at DNA nicks or breaks that result from abortive DNA ligati

84 ideal either for ligation (in case of a DNA-DNA nick) or for subsequent engagement by FEN1 (in case

85 nucleosomes containing discretely positioned DNA nicks, our evidence indicates that the ligase IIIalp

86 d by a pathway involving templated repair at DNA nicks rather than double-strand breaks.

87 ejections, which proceeded stepwise between DNA nicks, reaching a translocation speed of 75 kbp/s or

88 are critical for both nucleotide binding and DNA nick recognition.

89 c roles, as it contains both single-stranded DNA-nicking relaxase and ATP-dependent helicase domains

90 Mpa Lig has limited DNA nick-sealing activity but is efficient in ligating n

91 This small domain has been described as a DNA nick sensor, but it is not required for DNA nick joi

92 ific interaction between condensin I and the DNA nick-sensor poly(ADP-ribose) polymerase 1 (PARP-1).

93 polymerase the novel function of a molecular DNA nick-sensor, and that the DNA ligase can inhibit act

94 to the recruitment of PcrA-like helicases to DNA-nick sites and the processive translocation of the P

95 s DNA elements and strongly suggest that the DNA nicking step of V(D)J recombination can be rate limi

96 Recent findings suggest that DNA nicks stimulate homologous recombination by being co

97 tide nucleic acid (PNA)-directed design of a DNA-nicking system that enables selective and quantitati

98 gand-dependent enhancer activation, based on DNA nicking to relieve torsional stress from eRNA synthe

99 The relative number of DNA nicks to phosphodiester bonds in a circular plasmid

100 the MMR machinery is important in processing DNA nicks to produce double-stranded breaks, particularl

101 lated, topo I, and increased topo I-mediated DNA nicking under conditions of oxidative stress.

102 lation sites separating the mismatch and the DNA nick used to direct repair, from 10 to 808 base pair

103 a direct comparison of the thermodynamics of DNA nicks versus DNA gaps has not been performed.

104 that Ca(2+) and Mg(2+) promote single-strand DNA nicks, whereas Mn(2+) promotes double-strand DNA bre