ライフサイエンスコーパス: DNA packaging

1 identified bulge residues are important for DNA packaging.

2 FI protein (gpFI), is required for efficient DNA packaging.

3 e three-helix junction in motor assembly and DNA packaging.

4 UL25 minor capsid proteins are essential for DNA packaging.

5 the assembly of the ring ATPase that drives DNA packaging.

6 lude that the major role of IVa2 is in viral DNA packaging.

7 e loops have been proposed to play a role in DNA packaging.

8 alyzed, thus indicating a profound defect in DNA packaging.

9 membrane and optimal viral DNA synthesis and DNA packaging.

10 is required late in infection, during phage DNA packaging.

11 flexibility required for capsid assembly and DNA packaging.

12 to discriminate between lambda and 21 during DNA packaging.

13 mational changes can occur in the absence of DNA packaging.

14 moved to the surface of the compartment for DNA packaging.

15 d the affects they have on transcription and DNA packaging.

16 capsid, and precluded viral replication and DNA packaging.

17 ence of the DR1 element was also crucial for DNA packaging.

18 al genomes before and after, but not during, DNA packaging.

19 not be a static channel at the late stage of DNA packaging.

20 L1 52/55-kDa protein, which is required for DNA packaging.

21 energy to supplement the nanomotor-driven T4 DNA packaging.

22 termediate that was fully active in in vitro DNA packaging.

23 induces DNA compression and rotation during DNA packaging.

24 ase prior to subsequent shell maturation and DNA packaging.

25 pressure builds up inside the capsid during DNA packaging.

26 rough the portal vertex in the capsid during DNA packaging.

27 nd pUL28 interact with the portal to mediate DNA packaging.

28 ructures rather than having a direct role in DNA packaging.

29 supportive of a topological role in dynamic DNA packaging.

30 odecamers are central to capsid assembly and DNA packaging.

31 s: the initiation of procapsid formation and DNA packaging.

32 this center lead to a profound loss of phage DNA packaging.

33 ication between the domains is essential for DNA packaging.

34 enerated due to procapsid instability during DNA packaging.

35 eight-base recognition sequence involved in DNA packaging.

36 evance of these DNA binding activities to Ad DNA packaging.

37 equence specificity to initiate or terminate DNA packaging.

38 ctors play a role in the initiation of viral DNA packaging.

39 Maturation of the capsid occurs during DNA packaging.

40 he packaging motor, and for participation in DNA packaging.

41 contribute to stabilizing the P22 capsid for DNA packaging.

42 sembles onto the virion just before or after DNA packaging.

43 structural changes during self-assembly and DNA packaging.

44 esviruses to play an essential role in viral DNA packaging.

45 sembles sequentially on the viral head after DNA packaging.

46 e mechanisms involved in virion assembly and DNA packaging.

47 empty capsids, indicating a defect in viral DNA packaging.

48 tial for pRNA binding and thereby subsequent DNA packaging.

49 gene regulation, chromatin condensation and DNA packaging.

50 ne the function of the IVa2 protein in viral DNA packaging, a mutant virus that does not express the

51 ; it exhibits ATPase, nuclease, and in vitro DNA-packaging activities.

52 gp16-stimulated ATPase activity and in vitro DNA packaging activity, whereas their ATP binding and DN

53 lge, rather than its sequence, is primary in DNA packaging activity.

54 Asp255 mutants showed no detectable in vitro DNA packaging activity.

55 wild-type (WT) but exhibit little ATPase or DNA packaging activity.

56 e-specific cos cleavage activity, as well as DNA packaging activity; however, the heterotrimer is dep

57 individually or together, exhibited in vitro DNA-packaging activity, suggesting that communication be

58 ite a conserved role for histones as general DNA packaging agents, it is now clear that another key f

59 h has the potential to differentially affect DNA packaging among individuals.

60 latory mechanism for coupling DNA cutting to DNA packaging among the headful packaging nucleases from

61 ases into this feature resulted in a loss of DNA packaging and an impairment of initiation complex as

62 phage appears to encode proteins involved in DNA packaging and capsid assembly and presumably in host

63 y of the initial prohead I form and prior to DNA packaging and capsid expansion.

64 inor capsid proteins of HSV-1, implicated in DNA packaging and capsid maturation.

65 s solfataricus that has been associated with DNA packaging and chromatin regulation.

66 close mechanistic connection in vivo between DNA packaging and developmentally concurrent replication

67 nal surface of capsids and are essential for DNA packaging and DNA retention in the capsid, respectiv

68 ding protein as well as DNA, suggesting that DNA packaging and expulsion of the scaffolding protein a

69 for the pac2 T element in the initiation of DNA packaging and for the GC-rich motifs flanking the pa

70 ated protein that is involved in chromosomal DNA packaging and gene regulatory functions.

71 dle essential for genome stabilization after DNA packaging and implicated in Gram-negative cell envel

72 of the viral single-stranded DNA genome and DNA packaging and may play versatile roles in virus-host

73 motor by ensuring the correct orientation of DNA packaging and restricting initiation to a single eve

74 olution to coordinate the transition between DNA packaging and tail assembly.

75 sly that the peptide component mediates both DNA packaging and targeting of the nanoparticle while in

76 between the motor protomers is required for DNA packaging and that incorporation of even a single mu

77 like KUL25NS, had a block at a late stage of DNA packaging and that the packaged genome was shorter t

78 that the IVa2 protein plays a role in viral DNA packaging and that this function of the IVa2 protein

79 ids and that the C terminus is essential for DNA packaging and the production of infectious virus thr

80 ifying structural basis for coordinating the DNA packaging and transcription repression functions of

81 The dynamics of DNA packaging and unpackaging from the NCP affect all DN

82 he regulation of late gene expression, viral DNA packaging, and infectious virus production.

83 ration, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.

84 otein is incorporated into the capsid during DNA packaging, and previous studies of a null mutant hav

85 e number of theoretical studies have modeled DNA packaging, and the nature of the molecular dynamics

86 Central to DNA packaging are dynamic interactions among the packagi

87 particles in which expulsion of scaffold and DNA packaging are incomplete.

88 the terminase holoenzyme, DNA maturation and DNA packaging, are discussed.

89 e function of the J protein is to facilitate DNA packaging, as well as to mediate surface properties

90 A new phage T4 in vitro DNA packaging assay employed purified proheads, terminas

91 Rather, the density of DNA packaging at all endopolyploid levels in the linc1 l

92 'portal vertex' and consists of a portal, a DNA packaging ATPase and other components.

93 and hydrolyzes ATP despite the fact that the DNA packaging ATPase site in the N terminus of gpA has b

94 dV protein IVa2 is presumed to function as a DNA packaging ATPase.

95 changes in the region of gp6 exposed to the DNA-packaging ATPase and to gp15.

96 tein fold, an internal lipid membrane, and a DNA-packaging ATPase.

97 Unraveling the structure and assembly of the DNA packaging ATPases of the tailed double-stranded DNA

98 The DNA-packaging ATPases of various bacteriophages and euka

99 ort the complete atomic model of the headful DNA-packaging bacteriophage Sf6 at 2.9 A resolution dete

100 ee proteins are among six required for HSV-1 DNA packaging but dispensable for assembly of immature c

101 These cosB suppressors cause enhanced DNA packaging by 21-specific terminase and reduce packag

102 ata, in which electrostatic forces drive the DNA packaging by alternating between tensed and relaxed

103 ectly and specifically interferes with phage DNA packaging by blocking the phage terminase small subu

104 DNA packaging by double-stranded DNA bacteriophages and

105 DNA packaging by large DNA viruses such as the tailed ba

106 We go on to show how DNA packaging can induce spontaneous reorganization of t

107 similar volumes, consistent with comparable DNA packaging capacities.

108 variety of animal and human cells, has high DNA packaging capacity, and can conveniently be maintain

109 on dsDNA bacteriophages have revealed that a DNA packaging complex assembles at a special vertex call

110 nase cutting at cos, to generate the active, DNA packaging complex.

111 nanoscale viral channel at the late stage of DNA packaging could be a consequence of Brownian movemen

112 We have analyzed our DNA packaging data according to an N-step sequential min

113 These proheads also showed no DNA packaging defect in vitro as compared with WT.

114 he mild DNA packaging defects, a severe post-DNA packaging defect.

115 ransferred to the restrictive temperature, a DNA-packaging defect was evident.

116 mutant enzymes have, in addition to the mild DNA packaging defects, a severe post-DNA packaging defec

117 K84A enzyme had similar virion assembly and DNA packaging defects.

118 the nucleosome, in addition to its role as a DNA packaging device, is a signaling module through whic

119 ith its central domain and extends its 5'/3' DNA-packaging domain for gp16 binding.

120 nition of the viral genome for initiation of DNA packaging during virus assembly.

121 he role of electrostatic repulsion on single DNA packaging dynamics in bacteriophage phi 29 via optic

122 DNase I protection assay indicated that the DNA packaging efficiency of the VLF-1(Y355F) virus const

123 The DNA-packaging efficiency with dimeric pRNA was more seri

124 ested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses c

125 ng complexes and the dynamics of TerL during DNA packaging, endonuclease regulation, and motor mechan

126 The DNA packaging energy budget, i.e. DNA packaged/ATP hydro

127 Transcription factor A (TFAM) functions as a DNA packaging factor in mammalian mitochondria.

128 nd the high mobility group box transcription/DNA-packaging factor, h-mtTFA/TFAM.

129 However, recent single-particle DNA packaging force measurements have suggested that inc

130 dy we show that the L procapsid assembly and DNA packaging genes, which encode terminase, portal, sca

131 Even though DNA packaging has been studied extensively, currently no

132 ase the involvement of this motor protein in DNA packaging has recently been demonstrated.

133 of a 30-nm fiber as a basic folding unit for DNA packaging has remained a topic of active discussion.

134 ure composed of modules for DNA replication, DNA packaging, head and tail morphogenesis, and lysis.

135 tant post-translational modifications to the DNA packaging histones on the normal genome and the PSR

136 In the final stages of DNA packaging, however, the internal genome pressure dra

137 bserved in recent single-molecule studies of DNA packaging in bacteriophage lambda.

138 Here, we present measurements of viral DNA packaging in bacteriophage phi29 using an improved o

139 Double-stranded DNA packaging in bacteriophage T4 and other viruses occu

140 chanics models provide a detailed picture of DNA packaging in bacteriophage, revealing how conformati

141 Double-stranded DNA packaging in bacteriophages is driven by one of the

142 s inherent in the biological significance of DNA packaging in cell nuclei, as well as for gene therap

143 rence in the posttranslational regulation of DNA packaging in Eukarya vs. the Archaea.

144 lled nucleosomes, are the building blocks of DNA packaging in eukaryotic cells.

145 ht DNA bending is biologically important for DNA packaging in eukaryotic chromatin and tight DNA loop

146 lled protamines mediate the near crystalline DNA packaging in most vertebrate sperm cells.

147 nzyme complexes, which facilitate ATP-driven DNA packaging in phages and in many eukaryotic viruses,

148 tors were restarted to observe the motion of DNA packaging in real time.

149 Nase protection assay commonly used to study DNA packaging in several bacteriophage systems, such tha

150 functions of disulfide linkages for in vivo DNA packaging in sperm chromatin.

151 s have biological implications for protamine-DNA packaging in sperm heads.

152 common mechanism for the initiation step of DNA packaging in tailed double-stranded DNA bacterial vi

153 DNA packaging in the bacteriophage phi29 involves a mole

154 nown, it has been proposed to facilitate the DNA packaging in the prokaryotic nucleoid and/or to prom

155 the CCA bulge in this helix is essential for DNA packaging in vitro.

156 t structural compaction which may facilitate DNA packaging in vivo.

157 T3 phage capsids (ipDNA-capsids) produced by DNA packaging in vivo; some ipDNA has quantized lengths,

158 Evidence that in vivo bacteriophage T3 DNA packaging includes capsid hyper-expansion that is tr

159 chemical information, we propose a model for DNA packaging initiation.

160 three-dimensional pseudo-atomic model for a DNA-packaging initiation complex assembled from the term

161 erlying the forces and pressures involved in DNA packaging into bacteriophage capsids became the focu

162 nomic DNA and no appreciable change in viral DNA packaging into capsids.

163 ed by SaPIbov1 gene 12, which prevents phage DNA packaging into small capsids, and which is also prim

164 e not required for procapsid assembly or for DNA packaging into the structure, but that proteolysis i

165 enzyme thought to provide energy that drives DNA packaging into the virion during infection.

166 statements regarding my recent letter about DNA packaging into viral capsids.

167 e this assembly step is unique to the virus, DNA packaging is a novel target for the development of a

168 Adenovirus type 5 (Ad5) DNA packaging is initiated in a polar fashion from the l

169 Adenovirus (Ad) type 5 DNA packaging is initiated in a polar fashion from the l

170 The resulting DNA packaging is known to interfere with assembly of the

171 se, gp16, is also essential, but its role in DNA packaging is poorly understood.

172 A key event in DNA packaging is recognition of the viral DNA among othe

173 Finding that DNA packaging is unimpeded in proheads containing the fu

174 the terminal stage of viral DNA development, DNA packaging, is poorly understood.

175 To elucidate the mechanism of phi29 DNA packaging, it is critical to confirm whether pRNA bi

176 The bacteriophage T4 DNA packaging machine consists of a molecular motor asse

177 initiation and assembly of a supramolecular DNA packaging machine on the viral concatemer.

178 y filled with the viral genome by means of a DNA packaging machine situated at a special fivefold ver

179 ly steps reflecting formation of the active, DNA packaging machine.

180 ation of a precursor capsid that serves as a DNA packaging machine.

181 the energy transduction mechanism of a viral DNA packaging machine.

182 nnector, which may potentiate the release of DNA-packaging machine components, creating a binding sit

183 A model for the assembly of the T4 DNA-packaging machine is presented.

184 structure of the ATPase component in a phage DNA-packaging machine.

185 nizations and sequence conservation in their DNA packaging machinery and maturational proteases.

186 Thus the phi29 DNA packaging machinery tolerated nicks, but stopped at

187 ing in the retention of at least part of the DNA packaging machinery.

188 in interacting with tegument proteins or the DNA-packaging machinery at the portal vertex.

189 sophisticated strategies to hijack the phage DNA-packaging machinery for their own transfer.

190 ahedral procapsid, which then expands during DNA packaging (maturation).

191 Several models for the DNA packaging mechanism have been proposed, which attrib

192 s binding properties implicate a role in the DNA packaging mechanism.

193 al rotation requirement of some hypothetical DNA packaging mechanisms.

194 rocess but also can be useful in deciphering DNA-packaging mechanisms for other viruses belonging to

195 Indeed, studies on viral DNA packaging might lead to the development of new antiv

196 s, gp17, and DNA are actively engaged in the DNA packaging mode.

197 symmetry of the portal protein has prompted DNA packaging models in which ATP-driven portal protein

198 Recent DNA-packaging models proposed that the 5-fold procapsid

199 hat the packaging RNA of bacteriophage phi29 DNA packaging motor can be assembled from 3-6 pieces of

200 kaging RNA (pRNA) of the bacteriophage phi29 DNA packaging motor has been developed into a nanodelive

201 ing about possible applications of the viral DNA packaging motor in nanotechnology and medical applic

202 e of such a ring ATPase, the double-stranded-DNA packaging motor in the bacteriophage phi29.

203 plication of the pRNA-3WJ motif of the phi29 DNA packaging motor into fusion RNA with controllable an

204 is an essential component of the ATP-driven DNA packaging motor of bacteriophage o29.

205 (pRNA), which is a central component of the DNA packaging motor of bacteriophage phi29.

206 sequential action of the ATPase ring in the DNA packaging motor of bacteriophage varphi29 is regulat

207 The DNA packaging motor of the Bacillus subtilis bacteriopha

208 The DNA packaging motor of the bacteriophage varphi29, compr

209 We report the crystal structure of the DNA packaging motor protein, gene product 17 (gp17), in

210 The bacteriophage o29 DNA packaging motor that assembles on the precursor caps

211 acteriophage inside a bacterial host cell, a DNA packaging motor transports the viral DNA into the pr

212 n (3WJ) of the pRNA from bacteriophage phi29 DNA packaging motor were examined previously for ocular

213 This vertex is the site of assembly of the DNA packaging motor, and the connector has a central cha

214 ucleotide prohead RNA that is part of the 29 DNA packaging motor, at constant value of rf occurs with

215 sential component in the phi29 bacteriophage DNA packaging motor, the strongest biomolecular motor kn

216 system is the Bacillus subtilis phage phi 29 DNA packaging motor, which generates large forces to com

217 NA into its preformed procapsid requires the DNA packaging motor, which is the strongest known biolog

218 d operation of the powerful bacteriophage 29 DNA packaging motor.

219 f the self-assembling varphi29 bacteriophage DNA packaging motor.

220 t hexameric, pRNA ring in the context of the DNA packaging motor.

221 that forms the central channel of the phage DNA packaging motor.

222 to pRNA binding during assembly of an active DNA packaging motor.

223 ation is driven by an ATP hydrolysis-powered DNA packaging motor.

224 stalk domain are involved in assembling the DNA packaging motor.

225 The pRNA of bacteriophage phi29 DNA-packaging motor also forms a ring, but whether the p

226 to the assembly and function of a terminase DNA-packaging motor are discussed.

227 The central hub of the bacteriophage phi29 DNA-packaging motor contains a 3.6-nm channel for dsDNA

228 Here we report the structures of the DNA-packaging motor gp2 of bacteriophage Sf6, which reve

229 ults with respect to the assembly of a viral DNA-packaging motor is discussed.

230 Bacteriophage phi29 DNA-packaging motor is geared by a packaging RNA (pRNA)

231 We have recently found that the DNA-packaging motor of bacteriophage phi29 uses a third

232 third type of biomotor, including the viral DNA-packaging motor, beside the bacterial DNA translocas

233 The bacteriophage phi29 DNA-packaging motor, which allows double-stranded DNA to

234 of DNA translocation and cleavage in a viral DNA-packaging motor, which may be achieved via linker-do

235 Viral DNA packaging motors are among the most powerful molecul

236 has long been popularly believed that viral DNA packaging motors are rotation motors.

237 e findings support the hypothesis that viral DNA packaging motors contain an adenine-binding motif th

238 Bacteriophage DNA packaging motors translocate their genomic DNA into

239 rce generation is a common property of viral DNA packaging motors.

240 ility also occur in the study of other viral DNA-packaging motors.

241 velopment of antiviral drugs targeting viral DNA-packaging motors.

242 In the Phi29 bacteriophage, the DNA packaging nanomotor packs its double-stranded DNA ge

243 on of the channel of the bacteriophage phi29 DNA-packaging nanomotor and its subsequent incorporation

244 As a result of dense DNA packaging, NFs are resistant to nuclease degradation

245 ant role in a variety of contexts related to DNA packaging, nucleoprotein complex formation, and gene

246 ese data support a model in which adenovirus DNA packaging occurs via the formation of a IVa2 multipr

247 ATP-driven DNA packaging of the T4 capsid results in the loading of

248 studies were undertaken to determine whether DNA packaging or capsid assembly affected the pU(L)17/pU

249 branching, or damage repair concomitant with DNA packaging or cleavage.

250 on of a function of this protein relevant to DNA packaging other than its interaction with other term

251 We also mapped phage 9 g DNA packaging (pac) site containing two 21-bp direct rep

252 al DNA substrate, the very first step in the DNA packaging pathway, and stimulates the ATPase and pac

253 proteins suggest that they may have roles in DNA packaging, penton formation, and protein-protein int

254 d that the portal is required throughout the DNA packaging process, with different domains participat

255 tial function during the final stages of the DNA packaging process.

256 tely 50 atmospheres as a result of the phage DNA-packaging process.

257 ously reported to interact with the putative DNA packaging protein A32 in a yeast two-hybrid screen.

258 uting the coding region for the L1 52/55 kDa DNA packaging protein from HAdV-16.

259 genetic analysis indicated that L1 52/55-kDa DNA packaging protein may be associated with the higher

260 or capsid proteins VP5 and VP23, but not the DNA packaging protein pU(L)15.

261 bacteriophages encode a heterodimeric viral DNA packaging protein, called terminase.

262 53, each of these prophages encodes head and DNA packaging proteins related to the siphophage lambda

263 Since the presence and modification of these DNA-packaging proteins provide a scaffold for docking of

264 Of the three capsid-associated DNA-packaging proteins, UL17, UL25, and UL6, only UL17 a

265 mechanism of gpFI chaperone activity in the DNA packaging reaction of phage lambda.

266 termed the terminase, associates during the DNA packaging reaction.

267 t it reports directly on the kinetics of the DNA packaging reaction.

268 DNA bending is critical for DNA packaging, recognition, and repair, and occurs towar

269 However, this DNA packaging reduces accessibility of enzymes that act

270 DNA packaging requires an ATPase catalytic site situated

271 result of initiated but aborted attempts at DNA packaging, resulting in the retention of at least pa

272 -base pRNA supported the correct polarity of DNA packaging, selectively packaging the DNA left end.

273 ion could be further enhanced by using viral DNA packaging sequences (inverted terminal repeats [ITRs

274 terminally redundant and that initiation of DNA packaging series occurs across an approximately 1-kb

275 Analysis of in vivo initiation of the DNA packaging series showed that the Sf6 apparatus that

276 The DNA-packaging specificities of phages lambda and 21 depe

277 TerS, however, determines DNA packaging specificity but not the choice of large ve

278 es and many tailed bacteriophages includes a DNA packaging step where the viral genome is inserted in

279 f residues is incompatible with the observed DNA packaging step-size of approximately 2.5 bp, such th

280 by similarities in their capsid assembly and DNA packaging strategies.

281 ludes modules for lysogeny, DNA replication, DNA packaging, structural proteins, and host cell lysis.

282 l virus phi29 is the most efficient in vitro DNA packaging system, with which up to 90% of the added

283 The phi1026b genes for DNA packaging, tail morphogenesis, host lysis, integrati

284 ase subunit), UL23 (thymidine kinase), UL25 (DNA packaging tegument protein), and UL52 (helicase-prim

285 Many dsDNA viruses encode DNA-packaging terminases, each containing a nuclease dom

286 - and C-terminal domains, inhibited in vitro DNA packaging that is catalyzed by full-length gp17.

287 ion of cellular DNA, asymmetric division and DNA packaging, that are common to both endospore formati

288 Upon DNA packaging, the capsid lattice expands and becomes a

289 results suggest that, in the early stage of DNA packaging, the dsDNA genome is randomly distributed

290 At the late stage of DNA packaging, the negatively charged genome is increasi

291 DNA packaging using restriction digests of varphi29 DNA

292 te of ATP hydrolysis, 2.5- to 4.5-fold lower DNA packaging velocity, and required an activator protei

293 a requirement of the viral IVa2 protein for DNA packaging via a direct interaction with packaging se

294 The C18C19A20 bulge that is essential for DNA packaging was found to be dispensable for gp16 bindi

295 in and test whether it is indeed involved in DNA packaging, we constructed a recombinant vaccinia vir

296 a useful model for the study of herpesvirus DNA packaging, we investigated the effects of BDCRB on G

297 ction/hyper-expansion cycles that accelerate DNA packaging when packaging is slowed by increase in th

298 nces, a capsid expansion process accompanies DNA packaging, which significantly increases the volume

299 ulate gp17-ATPase, an activity essential for DNA packaging, while conversion back into oligomeric for

300 e observed, may allow phage T4 to coordinate DNA packaging with other ongoing processes, including vi