ライフサイエンスコーパス: DNA polymerase I

1 were extended with dATP by Escherichia coli DNA polymerase I.

2 not the Klenow fragment of Escherichia coli DNA polymerase I.

3 deficient large fragment of Escherichia coli DNA polymerase I.

4 ase-free Klenow fragment of Escherichia coli DNA polymerase I.

5 polymerase beta or Klenow fragment of E.coli DNA polymerase I.

6 eficient Klenow fragment of Escherichia coli DNA polymerase I.

7 ly in a crystal of the thermostable Bacillus DNA polymerase I.

8 th the equivalent region of Escherichia coli DNA polymerase I.

9 s, beta interacts with MutS, DNA ligase, and DNA polymerase I.

10 s by the Klenow fragment of Escherichia coli DNA polymerase I.

11 the proofreading domain of Escherichia coli DNA polymerase I.

12 (exo(-)) Klenow fragment of Escherichia coli DNA polymerase I.

13 exo-nuclease III and the Klenow fragment of DNA polymerase I.

14 atalytic site (motif A) of Thermus aquaticus DNA polymerase I.

15 oofreading by the Klenow fragment of E. coli DNA polymerase I.

16 reverse transcriptase, T7 DNA polymerase, or DNA polymerase I.

17 -3' exonuclease function of Escherichia coli DNA polymerase I.

18 e to restriction endonucleases, and notably, DNA polymerase I.

19 ge proteolytic fragment (Klenow fragment) of DNA polymerase I.

20 now fragment (KF exo-) from Escherichia coli DNA polymerase I.

21 prior work on the mechanism and dynamics of DNA polymerase I.

22 s and also by the Klenow fragment of E. coli DNA polymerase I.

23 sive sequence homology with Escherichia coli DNA polymerase I.

24 agment (KF, exo- mutant) of Escherichia coli DNA polymerase I.

25 gue, the Klenow fragment of Escherichia coli DNA polymerase I.

26 ly from the closely related Escherichia coli DNA polymerase I.

27 taricus Dpo4 and Bacillus stearothermophilus DNA polymerase I.

28 of palm domain containing proteins, such as DNA polymerase I.

29 ase-free Klenow fragment of Escherichia coli DNA polymerase I.

30 palm domains of phosphodiesterases, such as DNA polymerase I.

31 Arg(841) present in the fingers subdomain of DNA polymerase I.

32 Herpes simplex virus DNA polymerase is a heterodimer composed of a catalytic

33 Herpes simplex virus DNA polymerase is a heterodimer composed of UL30, a cata

34 Translesion synthesis (TLS) by Y-family DNA polymerases is a chief mechanism of DNA damage toler

35 FEN1 or the 5'-nuclease domains of bacterial DNA polymerases is a double-flap structure containing a

36 epair pathway requires the Vsr endonuclease, DNA polymerase I, a DNA ligase, MutS, and MutL to functi

37 ue of the small fragment of Escherichia coli DNA polymerase I, a FEN with which it shares 66% similar

38 elity of DNA replication by bacteriophage T4 DNA polymerase is achieved in a multiplicative process:

39 The high processivity of T7 DNA polymerase is achieved through tight binding to its

40 e critical viral replication gene (the viral DNA polymerase) is activated by E2F.

41 Therminator DNA polymerase is an efficient DNA-dependent TNA polymer

42 s less topo I was recruited, suggesting that DNA polymerase is an important modulator of the binding

43 DNA polymerase, a variant of the 9 degrees N DNA polymerase, is an efficient DNA-directed threosyl nu

44 iota (hPoliota), a member of the Y family of DNA polymerases, is an exception to these rules.

45 The Klenow fragment of DNA polymerase I (an A-family polymerase) can efficientl

46 e interaction between the Klenow fragment of DNA polymerase I and a series of defined oligonucleotide

47 ase active site motifs with Escherichia coli DNA polymerase I and bacteriophage T7 DNA polymerase.

48 the single strand break by endonuclease IV, DNA polymerase I and DNA ligase occurred and was not gre

49 nzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase.

50 nd break can be repaired by Escherichia coli DNA polymerase I and E. coli DNA ligase alone, though le

51 osite a single strand break, endonuclease IV DNA polymerase I and Escherichia coli DNA ligase are req

52 ve site and in different segments of E. coli DNA polymerase I and have determined the effects of thes

53 iously determined for the Klenow fragment of DNA polymerase I and is consistent with recent structura

54 and developed purification methods for whole DNA polymerase I and its 5'-3' exonuclease domain that a

55 A-binding proteins, the Klenow fragment from DNA polymerase I and Klenow exonuclease minus (which has

56 E. coli DNA polymerase I and Mycobacterium DinB1 extend the DNAp

57 amers did not inhibit the Klenow fragment of DNA polymerase I and only had a minor effect on RB69 DNA

58 e interaction between the Klenow fragment of DNA polymerase I and synthetic DNA primer-templates cont

59 sive sequence homology with Escherichia coli DNA polymerase I and T7 DNA polymerase, it contains uniq

60 of nucleotide repeats during replication by DNA polymerase I and that this action provides insight i

61 the Klenow fragment of the Escherichia coli DNA polymerase I and the Bacillus stearothermophilus pol

62 instead required polA and polC, which encode DNA polymerase I and the second DNA polymerase III enzym

63 combine single-molecule FRET with the use of DNA polymerase I and various fidelity mutants to highlig

64 ere also studied with the Klenow fragment of DNA polymerase I and with T4 DNA polymerase.

65 onucleases, especially those associated with DNA Polymerases I and III, affect inheritance of the sil

66 en verified by primer extension studies with DNA polymerases I and IV from E. coli.

67 riophage T4 DNA polymerase, Escherichia coli DNA polymerase I, and E.coli exonuclease III.

68 ists of UvrA, UvrB, UvrC, the UvrD helicase, DNA polymerase I, and ligase.

69 olymerization rate of the Klenow fragment of DNA polymerase I, and we demonstrate its combination wit

70 labeled using digoxigenin-11-dUTP and Klenow DNA polymerase-I, and detected using fluorescein isothio

71 I since very-long patch BER was inhibited by DNA polymerase I antibody and addition of excess DNA pol

72 m of the Klenow fragment of Escherichia coli DNA polymerase I as well as DNA polymerase of Thermus aq

73 small amounts of template by RCA using phi29 DNA polymerase is "background" DNA synthesis that usuall

74 tolerance to mutation in the active sites of DNA polymerases is being exploited to engineer polymeras

75 e or the Klenow fragment of Escherichia coli DNA polymerase I, both nucleotides failed to substitute

76 ort the crystal structure of a high fidelity DNA polymerase I bound to DNA primer-template caught in

77 bases can migrate through the run while the DNA polymerase is bound to the template-primer, or the D

78 ain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the po

79 modification does not affect the activity of DNA polymerase I, but four vinylphosphonate linkages in

80 In prokaryotes, this process is completed by DNA polymerase I by means of strand displacement DNA syn

81 The Klenow fragment of Escherichia coli DNA polymerase I catalyzes template-directed synthesis o

82 The addition of exogenous DNA polymerase I caused modest inhibition of BER, which

83 Accurate copying of the genome by DNA polymerases is challenging due in part to the contin

84 of DNA polymerse I in Escherichia coli, the DNA polymerase I class of enzymes has served as the prot

85 are presented for a Thermus aquaticus (Taq) DNA polymerase I complex (consisting of the protein, the

86 The human cytomegalovirus DNA polymerase is composed of a catalytic subunit, UL54,

87 DinB, a Y-family DNA polymerase, is conserved among all domains of life;

88 Bypass of DNA lesions by translesion DNA polymerases is conserved in bacteria, yeast, and mam

89 s relatives, our biochemical analysis of the DNA polymerase is consistent with the postulated nonther

90 We show that PolC is highly dynamic: this DNA polymerase is constantly recruited to and released f

91 ce present in the Mycobacterium tuberculosis DNA polymerase I corresponds to a hinge region in the fi

92 greatly stimulated when DNA synthesis by T7 DNA polymerase is coupled to DNA unwinding.

93 upled with the conformational transitions in DNA polymerases is critical for maintaining the fidelity

94 Fidelity of DNA synthesis, catalyzed by DNA polymerases, is critical for the maintenance of the

95 Klenow fragment (exo(-)) of Escherichia coli DNA polymerase I demonstrate that bypass of LdG modifica

96 ons with the 3'-5' exo(-) Klenow fragment of DNA polymerase I demonstrate the usefulness of our proto

97 Telomerase is the cellular RNA-dependent DNA polymerase (i.e. reverse transcriptase) that uses an

98 We found that a mutated form of KlenTaq DNA polymerase, i.e., KTqM747K, catalyzed O(6)-BnG adduc

99 uctures provide the first direct evidence in DNA polymerase I enzymes of a large conformational chang

100 ha (pol alpha) and Klenow fragment (exo-) of DNA polymerase I (Escherichia coli).

101 DNA polymerase alpha and Klenow fragment of DNA polymerase I (Escherichia coli).

102 e chemical step in the catalytic reaction of DNA polymerases is essential for elucidating the molecul

103 catalytic subunit of the eukaryotic B-family DNA polymerases is essential for the formation of active

104 n of UL42 and BMRF1 with their corresponding DNA polymerases is essential for viral DNA replication a

105 ass experiments performed in vitro utilizing DNA polymerase I exo-.

106 ther the 5' --> 3' exonuclease activities of DNA polymerase I, exonuclease VII, or RecJ.

107 cluding exonucleases I-IX (including the two DNA polymerase I exonucleases), RecJ exonuclease, SbcCD

108 ear antigen (PCNA), the auxiliary factor for DNA polymerase , is expressed zygotically in the develop

109 not present in other members of the E. coli DNA polymerase I family.

110 loop is not present in other members of the DNA polymerase I family.

111 of dATP and dGTP analogues to determine how DNA polymerase I from Bacillus stearothermophilus (BF),

112 tensive studies using the Klenow fragment of DNA polymerase I from E. coli (Kf) and found to be recog

113 tic experiments using the Klenow fragment of DNA polymerase I from E. coli suggest that a low dA misi

114 substrate by the Klenow fragment (exo(-)) of DNA polymerase I from E. coli.

115 The Klenow fragment of DNA polymerase I from Escherichia coli accepted Fapy.dGT

116 eolytic active site of the large fragment of DNA polymerase I from Escherichia coli have been elucida

117 reaction catalyzed by the Klenow fragment of DNA polymerase I from Escherichia coli.

118 c active site of the Klenow fragment (KF) of DNA polymerase I from Escherichia coli.

119 eal overdamped, coupled domain motion within DNA polymerase I from Thermus aquaticus (Taq polymerase)

120 The DNA polymerase I from Thermus aquaticus (Taq polymerase)

121 substrate recognition in the active site of DNA polymerase I from Thermus aquaticus (Taq) and select

122 designed based on two unique features of the DNA polymerase I gene (polA).

123 and has low homology with similar regions of DNA polymerase I gene from known microorganisms.

124 eaction (PCR) amplification of a T. pallidum DNA polymerase I gene.

125 a 260 bp region of the small fragment of the DNA polymerase I gene.

126 e binding established previously for several DNA polymerases is generally extended to all DNA polymer

127 onuclease activity of the Klenow fragment of DNA polymerase I has been investigated with a combinatio

128 olymerase is highly processive while E. coli DNA polymerase I has low processivity.

129 nd cannot grow at moderate temperatures, its DNA polymerase I has significant activity at 20-37 degre

130 dPTP) by exonuclease-free Klenow fragment of DNA polymerase I have been determined.

131 at positions 705 and 882 of Escherichia coli DNA polymerase I have been well established.

132 In accord with these roles, T7 DNA polymerase is highly processive while E. coli DNA po

133 The Rev1 DNA polymerase is highly specialized for the incorporati

134 ulgidus and Methanococcus jannaschii and the DNA polymerase I homologues from Thermus aquaticus and T

135 The Klenow fragment of Escherichia coli DNA polymerase I houses catalytic centers for both polym

136 sly, it has been shown that Escherichia coli DNA polymerases I, II, and III are incapable of bypassin

137 Escherichia coli DNA polymerases I, II, IV and V (UmuC) interact with bet

138 sing the exonuclease-free Klenow fragment of DNA polymerase I in a primer extension reaction.

139 propose a new in vivo function for wild-type DNA polymerase I in chromosome termination at site(s) no

140 t the crystal structure of Thermus aquaticus DNA polymerase I in complex with an inhibitory Fab, TP7,

141 the closed, open, and ajar conformations of DNA polymerase I in the binary (enzyme:DNA) state to bet

142 RNase D and the 3'-5' exonuclease domain of DNA polymerase I in the Werner syndrome gene product.

143 The Klenow exo- fragment of Escherichia coli DNA polymerase I incorporated 2'-deoxyadenosine (dA) six

144 Taq, the large fragment of Thermus aquaticus DNA polymerase I, incorporates a nucleotide opposite an

145 The vaccinia virus DNA polymerase is inherently distributive but acquires p

146 Although the vaccinia virus DNA polymerase is inherently distributive, a highly proc

147 Archaeal family-D DNA polymerase is inhibited by the presence of uracil in

148 forks or suppress futile origin firing when DNA polymerase is inhibited, leading to incomplete genom

149 ts demonstrate that the COOH terminus of the DNA polymerase is inserted into the subunit interface of

150 g single molecules of the Klenow fragment of DNA polymerase I into electronic nanocircuits allowed el

151 a also suggest that an aphidicolin-sensitive DNA polymerase is involved in the excision step of the n

152 tein, a eukaryotic member of the Y family of DNA polymerases, is involved in the tolerance of DNA dam

153 e Stoffel fragment (SF) of Thermus aquaticus DNA polymerase I is displayed on a filamentous phage by

154 Additionally, we identify that DNA polymerase I is important for both primed and naive

155 the thermodynamics of substrate selection by DNA polymerase I is important for characterizing the bal

156 However, in vivo, E. coli DNA polymerase I is involved primarily in the repair of

157 DNA polymerase I is proposed to fill DNA gaps during spa

158 to modulate DNA strand slippage synthesis by DNA polymerase I, is a wedge-shaped spirocyclic molecule

159 and previous observations that mitochondrial DNA polymerase is itself a reverse transcriptase, we pro

160 The Klenow fragment of Escherichia coli DNA polymerase I (KF) achieves this through a series of

161 uses the Klenow fragment of Escherichia coli DNA polymerase I (KF) and fluorescently labeled primer/t

162 with the Klenow fragment of Escherichia coli DNA polymerase I (KF) as a function of the concentration

163 use the Klenow fragment of Escherichia coli DNA polymerase I (KF) as a model proofreading polymerase

164 and the Klenow fragment of Escherichia coli DNA polymerase I (KF) incorporate all four nucleotide an

165 nt burst synthesis rate for Escherichia coli DNA Polymerase I (KF) was found to be an order of magnit

166 e and replicated with the Klenow fragment of DNA polymerase I (Kf).

167 erase (T7DNAP) and by the Klenow fragment of DNA polymerase I (KF).

168 sence of Klenow fragment of Escherichia coli DNA polymerase I (Kfexo(-)) and DNA polymerase beta (pol

169 mplates for a model enzyme, Escherichia coli DNA polymerase I Klenow fragment (exo-).

170 Here, individual DNA polymerase I Klenow fragment (KF) molecules were tet

171 A substrate between the pol and exo sites of DNA polymerase I Klenow fragment (KF).

172 However, DNA polymerase I Klenow fragment and avian myeloblastosi

173 E. coli DNA polymerase I Klenow fragment could employ 1-me-dATP

174 In the same constructs, Escherichia coli DNA polymerase I Klenow Fragment exo(-) is much less eff

175 a much better substrate for the model enzyme DNA polymerase I Klenow fragment lacking proofreading ac

176 tilizing the Escherichia coli UvrD helicase, DNA polymerase I Klenow fragment, two accessory proteins

177 an indirect assay employing Escherichia coli DNA polymerase I (Klenow enzyme) and poly(dT) template.

178 he dissociation constants for the binding of DNA polymerase I (Klenow fragment) (KF) to the primer-te

179 r extension by exonuclease-deficient E. coli DNA polymerase I (Klenow fragment) (KF) when (+)-trans-

180 e insertion by exonuclease-deficient E. coli DNA polymerase I (Klenow fragment) across from either th

181 ite-specific mutagenesis of Escherichia coli DNA polymerase I (Klenow fragment) and atomic substituti

182 of an exonuclease-deficient Escherichia coli DNA polymerase I (Klenow fragment) bound to primer-templ

183 rboxylate ligands, Asp(705) and Asp(882), of DNA polymerase I (Klenow fragment) in the early prechemi

184 mechanism of bypass by the Escherichia coli DNA polymerase I (Klenow fragment) in the presence of th

185 In particular, Arg668 of Escherichia coli DNA polymerase I (Klenow fragment) makes a critical cont

186 fer, we observed individual Escherichia coli DNA polymerase I (Klenow fragment) molecules performing

187 approach to monitor the movement of E. coli DNA polymerase I (Klenow fragment) on a DNA template dur

188 ts show that the binding of Escherichia coli DNA polymerase I (Klenow fragment) to a primer-template

189 of an exonuclease-deficient Escherichia coli DNA polymerase I (Klenow fragment) to DNA primer-templat

190 tudy the affinity and kinetics of binding of DNA Polymerase I (Klenow fragment) to DNA.

191 he mutant sites of duplex DNA was induced by DNA polymerase I (Klenow fragment) to preserve Watson-Cr

192 hree active-site mutants of Escherichia coli DNA polymerase I (Klenow fragment) were used to study DN

193 nsition that occurs when a binary complex of DNA polymerase I (Klenow fragment) with a primer-templat

194 lymerase ternary complex of Escherichia coli DNA polymerase I (Klenow fragment), containing the templ

195 dG adducts, positioned in the active site of DNA polymerase I (Klenow fragment), serve as donor fluor

196 The principle is based on DNA polymerase I (Klenow fragment)-induced coupling of t

197 n the structure of the exonuclease-deficient DNA polymerase I (Klenow fragment).

198 process were studied using Escherichia coli DNA polymerase I (Klenow fragment, 3'-exonuclease defici

199 mer extension reactions were performed using DNA polymerase I, Klenow fragment exo-.

200 s of the large fragment of Thermus aquaticus DNA polymerase I (Klentaq1) with a primer/template DNA a

201 Rev1's noncatalytic role in recruiting other DNA polymerases is known to be important for TLS.

202 The catalytic reaction mediated by DNA polymerases is known to require two Mg(II) ions, one

203 polymerase beta, a member of the X family of DNA polymerases, is known to be involved in base excisio

204 DNA polymerase I large (Klenow) fragment showed no detec

205 NA polymerases were investigated: mesophilic DNA polymerase I large (Klenow) fragment, 3'-->5' exo(-)

206 se (TteUvrD) and Bacillus stearothermophilus DNA polymerase I Large Fragment (Bstpol) using a coiled-

207 Nt.CviPII was used in combination with Bst DNA polymerase I large fragment to rapidly amplify anony

208 ures and enzyme kinetic analyses of Bacillus DNA polymerase I large fragment variants complexed with

209 able bacterial (Bacillus stearothermophilus) DNA polymerase I large fragments with DNA primer templat

210 novel polymerase from the well characterized DNA polymerase I-like Thermus thermophilus DNA polymeras

211 DNA polymerase is maintained at stalled replication fork

212 The interaction between UL2 and the DNA polymerase is mediated through the UL30 subunit.

213 DNA damage was also examined using DNA polymerase I-mediated biotin-dATP nick translation (

214 DNA polymerase I-mediated biotin-dATP nick translation (

215 Furthermore, we have found that the viral DNA polymerase is mislocalized to the cytoplasm in both

216 A mechanism by which the Klenow fragment of DNA polymerase I monitors the geometry of the base pairs

217 The polymerase and 5'-nuclease components of DNA polymerase I must collaborate in vivo so as to gener

218 We established a DNA polymerase I mutant library, with random substitutio

219 f mutation rates, we generated a panel of 66 DNA polymerase I mutants in Escherichia coli with compar

220 al proof that the D(752) allele in the viral DNA polymerase is necessary and sufficient for expressio

221 ther the Klenow fragment of Escherichia coli DNA polymerase I nor the T7 DNA polymerase, both of whic

222 Neither polA (DNA polymerase I) nor polB (DNA polymerase II) genes are

223 Although the REV3 translesion DNA polymerase is not required for recombination, it int

224 hich rat DNA polymerase beta substitutes for DNA polymerase I of Escherichia coli, we previously isol

225 We previously showed that the DNA polymerase I of Thermus aquaticus (TaqNP) endonucleo

226 Adenovirus DNA polymerase is one of three viral proteins and two ce

227 d by the Klenow fragment of Escherichia coli DNA polymerase I or by calf thymus DNA polymerase alpha.

228 Cells deleted for the polA (DNA polymerase I) or priA (primosome) genes are as sensi

229 Escherichia coli DNA polymerase I participates in DNA replication, DNA re

230 We demonstrated that the Klenow fragment (DNA polymerase I) performs translesion synthesis on this

231 The LEXE motif, conserved in eukaryotic type DNA polymerases, is placed close to the polymerization a

232 politana (Tne) DNA polymerase belongs to the DNA polymerase I (Pol I) family.

233 ts in the O-helix of Thermus aquaticus (Taq) DNA polymerase I (pol I) for altered fidelity of DNA syn

234 s ability to substitute for Escherichia coli DNA polymerase I (pol I) in the SC18-12 strain, which la

235 The 5' nuclease of DNA polymerase I (Pol I) of Escherichia coli is a member

236 DNA polymerase I (pol I) processes RNA primers during la

237 To increase error rates of DNA polymerase I (Pol I) replication, we introduced poin

238 elity of DNA replication by Escherichia coli DNA polymerase I (pol I) was assessed in vivo using a re

239 irectly visualize single fluorescent labeled DNA polymerase I (Pol) and ligase (Lig) molecules search

240 he 'non-replicative' DNA polymerases, namely DNA polymerase I (polA), II (polB), IV (dinB) and V (umu

241 expressed from an operon encoding a putative DNA polymerase I (PolA1), among other GAS products.

242 ditionally, deletion of the Klenow domain of DNA polymerase I (PolI) resulted in a approximately 35-f

243 In Bacillus subtilis, DNA polymerase is predominantly located at or near midce

244 Fidelity of DNA polymerases is predominantly governed by an induced

245 DNA polymerase mu (Polmu), an X-family DNA polymerase, is preferentially expressed in secondary

246 m that we previously deduced for Dpo4 and T7 DNA polymerases is preferred for Pol kappa as well, sugg

247 chanism for the observed expansions with Taq DNA polymerase is proposed that does not invoke strand s

248 The 5'-exonuclease domains of the DNA polymerase I proteins of Eubacteria and the FEN1 pro

249 ed to the 5' exonuclease domain of bacterial DNA polymerase I proteins.

250 Sequenase (exo-minus T7 DNA polymerase) is qualitatively similar to exo-minus Kl

251 support a model in which the leading strand DNA polymerase is recruited prior to origin DNA unwindin

252 the difference between low and high fidelity DNA polymerases is related to the efficiency of correct,

253 However, the RB69 DNA polymerase is relatively resistant to the broad-spec

254 The Polzeta translesion synthesis (TLS) DNA polymerase is responsible for over 50% of spontaneou

255 ed primarily in the repair of DNA whereas T7 DNA polymerase is responsible for the replication of the

256 polymerase I antibody and addition of excess DNA polymerase I reversed this inhibition.

257 The Klenow fragment of E. coli DNA polymerase I selects its natural substrates, deoxynu

258 Compared to the Klenow fragment of DNA polymerase I, Sequenase could read through homopolym

259 This BER process was dependent upon DNA polymerase I since very-long patch BER was inhibited

260 Primer utilization by T7 DNA polymerase is slower than primer formation.

261 that the Klenow fragment of Escherichia coli DNA polymerase I stopped synthesis mostly after incorpor

262 termining the biochemical properties of this DNA polymerase is structure-function studies of site-spe

263 sive (homologous or heterologous) "trailing" DNA polymerase is sufficient to improve gp41 processivit

264 oding the catalytic subunit of mitochondrial DNA polymerase is sufficient to support physiological va

265 With the Klenow fragment of Escherichia coli DNA polymerase I, synthesis stops at the base immediatel

266 200,000 members) of mutant Thermus aquaticus DNA polymerase I (Taq pol I) was created containing rand

267 200,000 members) of mutant Thermus aquaticus DNA polymerase I (Taq pol I) was created containing rand

268 DNA polymerases including Thermus aquaticus DNA polymerase I (Taq Pol), a thermostable enzyme common

269 s in the Klenow fragment of Escherichia coli DNA polymerase I that interact with the ssDNA overhang o

270 The Taq DNA polymerase is the most commonly used enzyme in DNA s

271 sequence analyses suggest that the class II DNA polymerase is the principal DNA replicative enzyme o

272 e nucleotidyl transfer reaction catalyzed by DNA polymerases is the critical step governing the accur

273 beta (polbeta), a member of the X family of DNA polymerases, is the major polymerase in the base exc

274 not a member of the Y family of error-prone DNA polymerases, is the primary mediator of survival thr

275 ith four polymerases, the Klenow fragment of DNA polymerase I, the Klenow fragment with the proof-rea

276 ses (the Klenow fragment of Escherichia coli DNA polymerase I, the modified T7 DNA polymerase (Sequen

277 In whole DNA polymerase I, the polymerase and 5'-nuclease activit

278 The Klenow fragment of Escherichia coli DNA polymerase I then elongates the template-primer by t

279 erase X (pol X), a member of the X family of DNA polymerases, is thought to be involved in base excis

280 distribution was influenced by the ratio of DNA polymerase I to DNA ligase activity in the reaction.

281 DB-dATP showed specific photocrosslinking of DNA polymerase I to DNA.

282 We also investigated the effect on DNA polymerase I to establish whether we could in the fu

283 -slide application of the Klenow fragment of DNA polymerase I to extend unmodified miRNAs hybridized

284 ditionally, Cu(II) chelated PyED outcompetes DNA polymerase I to successfully inhibit template strand

285 he ability of the Klenow fragment of E. coli DNA polymerase I to synthesize and extend the different

286 ne POLG, coding for the catalytic subunit of DNA polymerase, is typically proximal with early ophthal

287 tor of translesion DNA synthesis because the DNA polymerase is unable to extend beyond the incorporat

288 We demonstrate that the DNA polymerase is unable to extend beyond the incorporat

289 appa (Polkappa), a member of the Y-family of DNA polymerases, is unable to insert nucleotides opposit

290 It is also demonstrated that when Taq DNA polymerase is used in the presence of betaine or a p

291 ' exonuclease free (exo-) Klenow fragment of DNA polymerase I was used, dG-N2-3MeE promoted mostly on

292 with the Klenow fragment of Escherichia coli DNA polymerase I, we find that DDI markedly enhances the

293 lease reaction catalyzed by Escherichia coli DNA polymerase I, we have constructed expression plasmid

294 ce of the J-helix region of Escherichia coli DNA polymerase I, we performed site-directed mutagenesis

295 Various polymerization conditions using DNA polymerase I were examined to determine optimal labe

296 A by the Klenow fragment of Escherichia coli DNA polymerase I when individually substituted for its n

297 d by the Klenow fragment of Escherichia coli DNA polymerase I with better efficiency and fidelity tha

298 Although X-ray crystal structures of DNA polymerase I with substrate dNTPs have revealed key

299 fidelity Klenow fragment of Escherichia coli DNA polymerase I with the proofreading exonuclease activ

300 ween the Klenow fragment of Escherichia coli DNA polymerase I with the proofreading exonuclease inact