ライフサイエンスコーパス: DNA polymerase III

1 th more than just the chromosomal replicase, DNA polymerase III.

2 logy to the E. coli alpha subunit of E. coli DNA polymerase III.

3 the alpha and theta subunits in the E. coli DNA polymerase III.

4 lease proofreading subunit (DnaQ) of E. coli DNA polymerase III.

5 the clamp-loading subunit delta' of E. coli DNA polymerase III.

6 gene, which encodes the enzymatic subunit of DNA polymerase III.

7 olog of the Escherichia coli beta subunit of DNA polymerase III.

8 de insertion by the catalytic subunit of the DNA polymerase III.

9 ession of DnaA protein and the beta clamp of DNA polymerase III.

10 er spontaneous DSBs in a mutant with altered DNA polymerase III.

11 mediated by an unidentified modification of DNA polymerase III.

12 codes for both the tau and gamma subunits of DNA polymerase III.

13 ies a defect in proofreading activity of the DNA polymerase III.

14 naE homologue, encoding the alpha subunit of DNA polymerase III.

15 DNA polymerase III, a decameric 420-kDa assembly, simult

16 In addition, we have found that the DNA polymerase III alpha catalytic subunit interacts str

17 The crystal structure of Thermus aquaticus DNA polymerase III alpha subunit reveals that the struct

18 mined the influence of the 3' exonuclease of DNA polymerase III and exonuclease I on deletion via the

19 heir ability to inhibit purified B. subtilis DNA polymerase III and the growth of B. subtilis in cult

20 mp loader delta' subunit of Escherichia coli DNA polymerase III and the hexamerization component of N

21 The alpha subunits of bacterial DNA polymerase III and two distinct family X DNA polymer

22 DnaA boxes around the dnaN (beta subunit of DNA polymerase III) and dnaA genes usually defines the c

23 ved by the gamma-complex in Escherichia coli DNA polymerase III, and indeed our accessory subunit mod

24 a predicted function: dnaE2, a component of DNA polymerase III, and linB, which is similar to 1,3,4,

25 The tau and gamma subunits of DNA polymerase III are both encoded by a single gene in

26 e tau and gamma subunits of Escherichia coli DNA polymerase III, are suppressed by Cs,Sx mutations of

27 NA, UmuD interacts with the alpha subunit of DNA polymerase III at two distinct binding sites, one of

28 The prokaryotic DNA polymerase III beta homodimeric clamp links the repl

29 terial sliding clamp (SC), also known as the DNA polymerase III beta subunit, is an emerging antibact

30 dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta subunit, homolog of eukaryotic

31 f low-GC gram-positive organisms, be renamed DNA polymerase III C (Pol III C) to denote its origin fr

32 oli DNA polymerase II (B family) and E. coli DNA polymerase III (C family).

33 We conclude from this study that DNA polymerase III catalyzes translesion synthesis acros

34 The prokaryotic DNA polymerase III clamp loader complex loads the beta c

35 ect interaction between the alpha subunit of DNA polymerase III core and the beta sliding clamp.

36 plex or in the presence of gamma complex and DNA polymerase III core components.

37 preventing gamma complex from competing with DNA polymerase III core for binding a newly loaded beta.

38 primer, supports processive DNA synthesis by DNA polymerase III core in the presence of the beta slid

39 On model oligonucleotide templates, DNA polymerase III core is inhibited by SSB.

40 Nevertheless, identical assemblies of the DNA polymerase III core tethered to the beta sliding cla

41 cient overproduction and in vivo assembly of DNA polymerase III core, artificial operons containing t

42 In contrast, DNA polymerase III core, in the absence of deltadelta' a

43 theta is a tightly bound component of the DNA polymerase III core, which contains the alpha subuni

44 DNA polymerase III (DNA pol III) efficiently replicates

45 that inhibit the replication-specific enzyme DNA polymerase III (DNA pol III) of Staphlococcus aureus

46 Mutations that impair DNA polymerase III (DnaE, DnaQ subunits) or the replicat

47 structure, it is proposed that it be renamed DNA polymerase III E (Pol III E) to accurately reflect i

48 Only mutations in functions associated with DNA Polymerase III elevated deletion rates in our assays

49 which encode DNA polymerase I and the second DNA polymerase III enzyme, respectively.

50 of MMLV RT, including p53, Escherichia coli DNA polymerase III epsilon subunit, and the proofreading

51 dnaQ encodes DNA polymerase III epsilon subunit, the proofreading sub

52 the proofreading function carried out by the DNA polymerase III epsilon subunit.

53 ication misalignment events are sensitive to DNA polymerase III exonuclease, whereas SCE-associated e

54 Bacterial DNA polymerase III (family C DNA polymerase), the princi

55 ) and psi (psi) subunits of Escherichia coli DNA polymerase III form a heterodimer that is associated

56 e impaired in 3'-->5'exonuclease activity of DNA polymerase III, frame-shift mutagenesis increased 5-

57 a deletion approach on the alpha subunit of DNA polymerase III from Escherichia coli, we show that t

58 By purifying DNA polymerase III* from TSM-induced and control cells,

59 The Escherichia coli DNA polymerase III gamma complex clamp loader assembles

60 The Escherichia coli DNA polymerase III gamma complex loads the beta clamp on

61 bunit of the clamp-loader complex of E. coli DNA polymerase III has been determined.

62 Mg2+ in the active site of Escherichia coli DNA polymerase III have been identified as Asp401, Asp40

63 icating that tau's unique ability to bind to DNA polymerase III holding chipsi in the same complex is

64 le of accessory subunits of Escherichia coli DNA polymerase III holoenzyme (HE) in determining chromo

65 The in vitro fidelity of Escherichia coli DNA polymerase III holoenzyme (HE) is characterized by a

66 The DNA polymerase III holoenzyme (HE) is the primary replic

67 The DNA polymerase III holoenzyme (HE) is the primary replic

68 There is a controversy as to whether or not DNA polymerase III holoenzyme (Pol III HE) contains gamm

69 or subassemblies of the bacterial replicase, DNA polymerase III holoenzyme (Pol III HE).

70 uires the UmuD'2C complex, RecA protein, and DNA polymerase III holoenzyme (pol III).

71 trand DNA replication in the presence of the DNA polymerase III holoenzyme all had the same protein c

72 subunit of the Escherichia coli replicative DNA polymerase III holoenzyme and encodes an exonuclease

73 freading 3' exonuclease (epsilon subunit) of DNA polymerase III holoenzyme and is a critical determin

74 Interaction between the tau subunit of the DNA polymerase III holoenzyme and the DnaB helicase is c

75 t between the dimeric tau subunit within the DNA polymerase III holoenzyme and the DnaB helicase is r

76 tant for DNA replication, the chi subunit of DNA polymerase III holoenzyme and the PriA helicase.

77 g clamp protein beta of the Escherichia coli DNA polymerase III holoenzyme are shown to function as t

78 The single endogenous DnaX complex within DNA polymerase III holoenzyme assembles beta onto both t

79 We demonstrate here that the E. coli DNA polymerase III holoenzyme assembles into a particle

80 The gamma complex of the Escherichia coli DNA polymerase III holoenzyme assembles the beta sliding

81 Escherichia coli DNA polymerase III holoenzyme contains 10 different subu

82 The catalytic core of Escherichia coli DNA polymerase III holoenzyme contains three subunits: a

83 pose a model in which strand displacement by DNA polymerase III holoenzyme depends upon a Pol III-tau

84 The minimal Tth DNA polymerase III holoenzyme displays an elongation rat

85 The tau and gamma proteins of the DNA polymerase III holoenzyme DnaX complex are products

86 The DNA Polymerase III holoenzyme forms initiation complexes

87 ed the in vitro fidelity of Escherichia coli DNA polymerase III holoenzyme from a wild-type and a pro

88 ng the poorly conserved delta subunit of the DNA polymerase III holoenzyme from all sequenced bacteri

89 Subunit interactions known to occur in DNA polymerase III holoenzyme from mesophilic bacteria i

90 SB's association with the chi subunit of the DNA polymerase III holoenzyme has been proposed to confe

91 components and functions of Escherichia coli DNA polymerase III holoenzyme have been studied extensiv

92 We report here that the DNA polymerase III holoenzyme in a stalled E. coli repli

93 Purified gp8 inhibits DNA polymerization by DNA polymerase III holoenzyme in vitro by interfering wi

94 Probing with surface plasmon resonance, DNA polymerase III holoenzyme initiation complexes were

95 ngs are consistent with chipsi strengthening DNA polymerase III holoenzyme interactions with the SSB-

96 Escherichia coli DNA polymerase III holoenzyme is a multisubunit composit

97 processive DNA synthesis by Escherichia coli DNA polymerase III holoenzyme is achieved by the direct

98 The DNA polymerase III holoenzyme is composed of 10 subunits

99 The DnaX complex subassembly of the DNA polymerase III holoenzyme is comprised of the DnaX p

100 rse of this work, we discovered that E. coli DNA polymerase III holoenzyme is itself capable of carry

101 ion of the theta subunit of Escherichia coli DNA polymerase III holoenzyme is not well established.

102 DNA polymerase III holoenzyme is responsible for chromos

103 lity DNA synthesis that is characteristic of DNA polymerase III holoenzyme is the 3'-->5' proofreadin

104 DNA polymerase III holoenzyme is the major replicative e

105 Escherichia coli DNA polymerase III holoenzyme is the replicative enzyme

106 e in their interaction with the helicase and DNA polymerase III holoenzyme lacking the tau subunit so

107 imers are formed, two molecules of the large DNA polymerase III holoenzyme machinery assemble into th

108 e polymerase core (alphaepsilontheta) of the DNA polymerase III holoenzyme map to widely separated lo

109 Studies of the DNA polymerase III holoenzyme of Escherichia coli suppor

110 essive replication reaction catalyzed by the DNA polymerase III holoenzyme on single-stranded DNA tem

111 med in the absence of the tau subunit of the DNA polymerase III holoenzyme produce shorter leading an

112 esults imply that T. thermophilis utilizes a DNA polymerase III holoenzyme replication machinery simi

113 tadelta'chi psi) within the Escherichia coli DNA polymerase III holoenzyme serves to load the dimeric

114 beads to investigate the effects of various DNA polymerase III holoenzyme subunits on the kinetics o

115 ainst the alpha catalytic subunit of E. coli DNA polymerase III holoenzyme to distinguish a novel pol

116 mp encircles the primer-template and tethers DNA polymerase III holoenzyme to DNA for processive repl

117 for DnaB binding, which leads to assembly of DNA polymerase III holoenzyme to form the replisome.

118 interaction with the alpha subunit, allowing DNA polymerase III holoenzyme to synthesize both leading

119 beta sliding clamp encircles DNA and tethers DNA polymerase III holoenzyme to the template for high p

120 These ring-shaped clamps tether DNA polymerase III holoenzyme to the template, facilitat

121 u subunit of the replicative polymerase (the DNA polymerase III holoenzyme) and the replication fork

122 aA operon, including DnaA, the beta clamp of DNA polymerase III holoenzyme, and RecF.

123 dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF.

124 , encoding the tau and gamma subunits of the DNA polymerase III holoenzyme, as a high-copy suppressor

125 In Escherichia coli DNA polymerase III holoenzyme, chi and Psi are tightly a

126 The E. coli replicase, DNA polymerase III holoenzyme, contains two polymerases

127 m of the E. coli replicative DNA polymerase, DNA polymerase III holoenzyme, function in translesion s

128 the editing function of the Escherichia coli DNA polymerase III holoenzyme, i.e., the DnaQ/MutD prote

129 codes both the gamma and tau subunits of the DNA polymerase III holoenzyme, identified the dnaX trans

130 Studies of the E. coli replicase, DNA polymerase III holoenzyme, in chain elongation with

131 as measured by processive replication by the DNA polymerase III holoenzyme, indicates a minimal requi

132 oteins promote the engagement of primase and DNA polymerase III holoenzyme, initiating semi-discontin

133 ocessive DNA replication by Escherichia coli DNA polymerase III holoenzyme, is assembled onto DNA by

134 The Escherichia coli chromosomal replicase, DNA polymerase III holoenzyme, is highly processive duri

135 codes both the gamma and tau subunits of the DNA polymerase III holoenzyme, is subject to cell cycle

136 Here we show that delta, a single subunit of DNA polymerase III holoenzyme, opens beta and slips it o

137 ta' subunits are essential components of the DNA polymerase III holoenzyme, required for assembly and

138 , encoding the tau and gamma subunits of the DNA polymerase III holoenzyme, that causes extreme cell

139 a specialized multicomponent replicase, the DNA polymerase III holoenzyme, that consist of three ess

140 The epsilon subunit of Escherichia coli DNA polymerase III holoenzyme, the enzyme primarily resp

141 nd gamma, are found co-assembled in purified DNA polymerase III holoenzyme, the pathway of assembly i

142 as a sliding clamp to tether the replicase, DNA polymerase III holoenzyme, to DNA.

143 showed that the replicative polymerase, the DNA polymerase III holoenzyme, was indeed a dimer with t

144 sivity factor beta(2) onto primed DNA in the DNA polymerase III holoenzyme-catalyzed reaction.

145 esistance to high levels of glutamate on the DNA polymerase III holoenzyme-catalyzed reaction.

146 mp loader, RFC, and the structure of E. coli DNA polymerase III holoenzyme.

147 the catalytic alpha subunit of the bacterial DNA Polymerase III holoenzyme.

148 t is part of the Escherichia coli replicase, DNA polymerase III holoenzyme.

149 osely links primase action with extension by DNA polymerase III holoenzyme.

150 eplication of the Escherichia coli genome by DNA polymerase III holoenzyme.

151 er for the synthesis of DNA chain by E. coli DNA polymerase III holoenzyme.

152 s to form the E. coli chromosomal replicase, DNA polymerase III holoenzyme.

153 composed solely of E. coli DnaB helicase and DNA polymerase III holoenzyme.

154 dnaX gene, the gamma and tau subunits of the DNA polymerase III holoenzyme.

155 d, as was their ability to interact with the DNA polymerase III holoenzyme.

156 ctions as a sliding clamp for the replicase, DNA polymerase III holoenzyme.

157 freading epsilon subunit of Escherichia coli DNA polymerase III holoenzyme.

158 ene, encoding the alpha-catalytic subunit of DNA polymerase III holoenzyme.

159 k formation by the restart primosome and the DNA polymerase III holoenzyme.

160 on and abolished the replication activity of DNA polymerase III holoenzyme.

161 nits of the DnaX complex of Escherichia coli DNA polymerase III holoenzyme: tau, gamma, delta, delta'

162 The delta' subunit of the DNA polymerase-III holoenzyme is a key component of the

163 on and abolished the replication activity of DNA polymerase-III holoenzyme.

164 We show that the alpha polymerase subunit of DNA polymerase III interacts with the beta-clamp via its

165 Replication by Escherichia coli DNA polymerase III is disrupted on encountering DNA dama

166 To determine whether DNA polymerase III is involved in the TSM phenotype, we

167 These findings suggest that DNA polymerase III is modified in TSM-induced cells.

168 e 28 kDa epsilon subunit of Escherichia coli DNA polymerase III is the exonucleotidic proofreader res

169 s techniques to identify the region(s) where DNA polymerase III-mediated replication has historically

170 )C with the epsilon and the beta subunits of DNA polymerase III might help to regulate DNA replicatio

171 dnaX encoding the gamma and tau subunits of DNA polymerase III mimic dnaK phenotypes and are genetic

172 dGTP analogues which selectively inhibit the DNA polymerase III of Bacillus subtilis and other Gram-p

173 re required for binding of the beta-clamp to DNA polymerase III of Escherichia coli, a polymerase of

174 freading epsilon-subunit of Escherichia coli DNA polymerase III on the genetic instabilities of the C

175 he hydrophobic cleft on the clamp that binds DNA polymerase III or a clamp-binding motif in SocB.

176 notype is mediated through a modification of DNA polymerase III or the activation of a previously uni

177 lication activity and that a modification of DNA polymerase III or the expression of a previously uni

178 e-stranded binding protein (SSB), and either DNA polymerases III or II.

179 presence of an antimutator allele of E. coli DNA polymerase III (Pol III) (dnaE915), but not in the p

180 rains carrying both an antimutator allele of DNA polymerase III (Pol III) alpha-subunit (dnaE915) and

181 The dnaX gene encodes the tau subunit of DNA polymerase III (Pol III) holoenzyme, the enzyme resp

182 ction of the dnaX-encoded tau subunit of the DNA polymerase III (Pol III) holoenzyme.

183 The fidelity of Escherichia coli DNA polymerase III (pol III) is measured and the effects

184 DNA polymerase III (Pol III) is the catalytic alpha subu

185 DNA polymerase III (pol III) of Gram-positive eubacteria

186 nce loaded, beta(148-152) was proficient for DNA polymerase III (Pol III) replication in vitro.

187 ene, encoding the catalytic alpha-subunit of DNA polymerase III (pol III) was isolated by genetic com

188 nts (1-2 kb) and utilize a highly processive DNA polymerase III (pol III), which is held to DNA by a

189 ucleolytic proofreading (epsilon) subunit of DNA polymerase III (Pol III).

190 ucture of the catalytic alpha-subunit of the DNA polymerase III (Pol IIIalpha) holoenzyme bound to pr

191 the alpha (catalytic) subunit of replicative DNA polymerase III (PolIII).

192 The epsilon subunit of Escherichia coli DNA polymerase III possesses 3'-exonucleolytic proofread

193 s of Nuc and epsilon186, an Escherichia coli DNA polymerase III proofreading domain, providing a comm

194 ivated form of UmuD (UmuD'), UmuC, RecA, and DNA polymerase III proteins.

195 erial DnaA (replication initiator) and DnaE (DNA polymerase III) proteins respectively.

196 sistent with the in vivo requirement for two DNA polymerase III replicases for B. subtilis chromosoma

197 oteomic/genomic approach to characterize the DNA polymerase III replication apparatus of the extreme

198 DnaB is the helicase associated with the DNA polymerase III replication fork in Escherichia coli.

199 Escherichia coli DNA polymerase III subunits tau and gamma are produced f

200 nsfer determinants and genes encoding likely DNA polymerase III subunits, but it lacks an obvious can

201 s as its mechanistic basis the alteration of DNA polymerase III such that it becomes capable of repli

202 ssion of the epsilon proofreading subunit of DNA polymerase III suppresses umuDC-mediated cold sensit

203 The Escherichia coli DNA polymerase III tau and gamma subunits are single-str

204 mbly of tau and gamma, suggesting a role for DNA polymerase III' [tau(2)(pol III core)(2)] in the ass

205 , the CDC2 gene encodes the large subunit of DNA polymerase III, the analogue of mammalian DNA polyme

206 rocessivity of the isolated alpha subunit of DNA polymerase III, the product of the dnaE gene, from t

207 e P1 can substitute for the Escherichia coli DNA polymerase III theta subunit, as evidenced by its st

208 tau binds DNA polymerase III tightly; gamma does not.

209 ent per cell, thus allowing partially active DNA polymerase III to complete replication of enough chr

210 The beta clamp tethers DNA polymerase III to DNA and enhances the efficiency of

211 , UmuD' acts in concert with UmuC, RecA, and DNA polymerase III to facilitate the process of transles

212 portion of the catalytic subunit (alpha) of DNA polymerase III to the C-terminal half, downstream of

213 proteins in this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC (umu: UV mutage

214 The tau subunit dimerizes DNA polymerase III via interaction with the alpha subuni

215 ts, UmuD and UmuD' interact differently with DNA polymerase III: whereas uncleaved UmuD interacts mor

216 umption of DNA synthesis occurs by replacing DNA polymerase III, which is bound to DNA by the beta-sl

217 rase II and inhibits proofreading by E. coli DNA polymerase III, while permitting templated DNA synth

218 c C-tau binds the alpha catalytic subunit of DNA polymerase III with a 1:1 stoichiometry.

219 vitro studies of the interactions of E. coli DNA polymerase III with these adducts have also been und