ライフサイエンスコーパス: DNA polymerase alpha

1 duced expression of the catalytic subunit of DNA polymerase alpha.

2 cation including DHFR, thymidine kinase, and DNA Polymerase alpha.

3 zed several temperature-sensitive mutants of DNA polymerase alpha.

4 h chromatin is dependent on RNA synthesis by DNA polymerase alpha.

5 proteins, the origin recognition complex and DNA polymerase alpha.

6 Pol1p promotes C(1-3)A strand resynthesis by DNA polymerase alpha.

7 continuous DNA synthesis was attributable to DNA polymerase alpha.

8 chia coli DNA polymerase I or by calf thymus DNA polymerase alpha.

9 er is sufficient for extension with dNTPs by DNA polymerase alpha.

10 xDNA uncovered a gene encoding a subunit of DNA polymerase alpha.

11 was inhibited by neutralizing antibodies to DNA polymerase alpha.

12 pin loop in the template and dissociation of DNA polymerase alpha.

13 e replication without causing degradation of DNA polymerase alpha.

14 Mcm2-7 DNA helicase at replication forks to DNA polymerase alpha.

15 ts of the RPC is crucial to couple MCM2-7 to DNA polymerase alpha.

16 on of the error-prone synthesis catalyzed by DNA polymerase alpha.

17 xpression of Pol1p, the catalytic subunit of DNA polymerase alpha.

18 a180, which encodes the catalytic subunit of DNA polymerase alpha.

19 p58, and is normally tightly associated with DNA polymerase alpha.

20 actin I, alpha-telomere binding protein, and DNA polymerase alpha.

21 OLA1, which encodes the catalytic subunit of DNA polymerase-alpha.

22 th an antibody that specifically neutralized DNA polymerase alpha activity.

23 yotes, but instead functions like eukaryotic DNA polymerase alpha, adding a stretch of deoxynucleotid

24 We have used DNA polymerase alpha affinity chromatography to identify

25 Low levels of DNA polymerase-alpha also induced very high rates of ane

26 Hypotrich phylogenies based on DNA polymerase alpha amino acid sequences are incongruen

27 Phylogenetic relationships inferred from DNA polymerase alpha amino acid sequences have been used

28 pe 1 reverse transcriptase but not for human DNA polymerase alpha and beta.

29 ant effect on the synthetic ability of human DNA polymerase alpha and delta by Tim-Tipin was observed

30 xposure to aphidicolin, an inhibitor of both DNA polymerase alpha and delta, results in a reproducibl

31 Furthermore, the addition of DNA polymerase alpha and deoxynucleoside triphosphates t

32 olin at concentrations specific for blocking DNA polymerase alpha and dideoxynucleotide triphosphates

33 rmine if two B family DNA polymerases, human DNA polymerase alpha and herpes simplex virus I DNA poly

34 eukaryotic primases that form a complex with DNA polymerase alpha and its accessory B subunit.

35 nd 1,3-diaza-2-oxophenoxazine (tCo) by human DNA polymerase alpha and Klenow fragment of DNA polymera

36 treatment with aphidicolin, an inhibitor of DNA polymerase alpha and other polymerases.

37 lular proteins to the origin, including host DNA polymerase alpha and replication protein A.

38 for the initiation of SV40 DNA replication, DNA polymerase alpha and SV40 large T antigen.

39 T antigen, DNA polymerase alpha and the activation domain of VP16 a

40 wn-regulated by miR-206, the p180 subunit of DNA polymerase alpha and three other genes are shown to

41 g Asf1p are highly sensitive to mutations in DNA polymerase alpha and to DNA replicational stresses.

42 ntrol the onset of DNA synthesis mediated by DNA polymerase-alpha and its intrinsic RNA primase activ

43 fragment of DNA polymerase I, DNA Sequence, DNA polymerase-alpha and polymerase-beta, as well as HIV

44 ore helicase, the lagging strand polymerase, DNA polymerase-alpha and the replication clamp, prolifer

45 We demonstrated a requirement for DNA polymerases alpha and beta in repair of both photopr

46 Mammalian DNA polymerases alpha and beta lack 3' exonuclease activ

47 transcriptases and compared them to those of DNA polymerases alpha and beta.

48 titive inhibition studies indicate that like DNA polymerases alpha and delta (pol alpha and pol delta

49 permitted us to determine that the essential DNA polymerases alpha and delta and DNA primase were req

50 DNA polymerases alpha and delta carry out the initiation

51 DNA at an origin of replication, stimulates DNA polymerases alpha and delta, and supports the format

52 The MRC were shown to contain DNA polymerases alpha and delta, DNA primase, DNA helica

53 of archaeal DNA polymerase II and eukaryotic DNA polymerases alpha and delta, is shown to belong to t

54 xpected, the 3dMeA lesion blocked both human DNA polymerases alpha and delta.

55 at contains Mrc1, Tof1, MCM-Cdc45, GINS, and DNA polymerases alpha and epsilon and that recruits the

56 However, due to the presence of DNA polymerases alpha and epsilon in the depleted extrac

57 bstantially blocked further DNA synthesis by DNA polymerases alpha and epsilon in vitro, indicating a

58 edly stimulates the polymerase activities of DNA polymerases alpha and epsilon in vitro.

59 polymerase delta but was free of detectable DNA polymerases alpha and epsilon.

60 extending DNA strand catalyzed by both human DNA polymerases alpha and epsilon.

61 ase are dependent on RNA primer synthesis by DNA polymerase alpha, and it has been suggested that the

62 ut5 was required for the recruitment of Atr, DNA polymerase alpha, and Rad1 but not RPA to chromatin

63 the progression of S-phase, such as DHFR and DNA polymerase alpha, and they play a critical role in c

64 origin unwinding and the loading of RPA and DNA polymerase alpha are also inhibited.

65 luding CDK2, Cdc7, and Cdc45, but not RPA or DNA polymerase alpha, are necessary for activating the d

66 DNA polymerase alpha association is similarly delayed at

67 primarily blocked replication by calf thymus DNA polymerase alpha at the modified base, while human p

68 r extension reactions catalyzed by mammalian DNA polymerases alpha, beta, and delta.

69 r extension reactions catalyzed by mammalian DNA polymerases alpha, beta, and delta.

70 y primer extension in reactions catalyzed by DNA polymerases alpha, beta, and delta.

71 (as measured by DNA polymerase activity) of DNA polymerases alpha, beta, delta (+/- PCNA) and straig

72 These SLE sera did not inhibit the DNA polymerases alpha, beta, gamma and had no antibody t

73 ha does not, indicating that Cdc45, RPA, and DNA polymerase alpha bind chromatin sequentially at the

74 ituents of the replication fork, such as the DNA polymerase alpha-binding protein Ctf4, contribute to

75 ylation of RPA disrupts the interaction with DNA polymerase alpha but has no significant effect on th

76 DNA polymerase alpha, but not T antigen also interacted

77 d through S phase; and (b) the inhibition of DNA polymerase alpha by aphidicolin-blocked DNA damage.

78 Here, we show that both Klenow fragment and DNA polymerase alpha can utilize 8-oxodGTP and incorpora

79 NA replication gene: the structural gene for DNA polymerase alpha (cdc17/pol1).

80 of the four yeast family B DNA polymerases, DNA polymerase alpha, delta, epsilon or zeta, results in

81 res, interacts directly with the replicative DNA polymerases (alpha, delta, and epsilon), and markedl

82 nriched with replication proteins, including DNA polymerases (alpha, delta, etc.), was isolated, whic

83 MCM-GINS (CMG) replicative DNA helicase with DNA polymerases alpha, delta, and epsilon and other prot

84 the efficiencies with which the replicative DNA polymerases alpha, delta, and epsilon incorporated t

85 Accurate DNA synthesis by the replicative DNA polymerases alpha, delta, and epsilon is critical fo

86 h alanine in the catalytic subunits of yeast DNA polymerases alpha, delta, epsilon, and zeta and exam

87 Aphidicolin, an inhibitor of DNA polymerase alpha, did not prevent camptothecin-induc

88 e (TAK)1, TAB1, c-FOS, DNA topoisomerase II, DNA polymerase alpha, dihydrofolate reductase, thymidine

89 gene encoding the large catalytic subunit of DNA polymerase alpha (DNA pol alpha) in the ciliate Oxyt

90 roximately 40 proteins of the MRC, including DNA polymerase alpha (DNA pol alpha), DNA topoisomerase

91 No evidence for the binding of DNA polymerase alpha, DNA ligase I or topoisomerase II w

92 , some of which were further confirmed to be DNA polymerase alpha, DNA topoisomerase I, and PCNA by i

93 prevented, Cdc45 binds to chromatin whereas DNA polymerase alpha does not, indicating that Cdc45, RP

94 Macronuclear DNA clones of DNA polymerase alpha encoding approximately 1000 amino a

95 Human DNA polymerase alpha extended DNA primers terminated by

96 This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation

97 DNA polymerase-alpha fits this purpose exactly, but litt

98 roliferating cell nuclear antigen (PCNA) and DNA polymerase alpha from replication forks.

99 eptide was able to displace the Ctf4 partner DNA polymerase alpha from the replisome in yeast extract

100 ation of a 5'-3' exonuclease associated with DNA polymerase alpha from the yeast Saccharomyces cerevi

101 lagging-strand DNA synthesis by facilitating DNA polymerase alpha function at replication forks.

102 synthesizes short RNA oligonucleotides that DNA polymerase alpha further elongates in order to initi

103 A primase synthesizes short RNA primers that DNA polymerase alpha further elongates.

104 ort the complex pattern of scrambling in the DNA polymerase alpha gene of Stylonychia lemnae.

105 An example is the extensively scrambled DNA polymerase alpha gene that is broken into 48 pieces

106 The complete macronuclear DNA polymerase alpha gene, previously sequenced in Oxytr

107 lex phenomenon during evolution, we examined DNA polymerase alpha genes in several earlier diverging

108 response only after the DNA was unwound and DNA polymerase alpha had been loaded.

109 e of ara-C and CdA on DNA extension by human DNA polymerase alpha in an in vitro model system was con

110 M helicase enhance the physical stability of DNA polymerase alpha in the absence of their presumed li

111 Our results demonstrate a role of DNA polymerase alpha in the establishment of silencing.

112 on protein (PRP) complex that interacts with DNA polymerase alpha in the lagging strand of DNA during

113 also show that aRPA stimulates synthesis by DNA polymerase alpha in the presence of PCNA and RFC.

114 d other heterochromatin loci, interacts with DNA polymerase alpha in vivo and in vitro in wild type c

115 induced by reduced levels of the replicative DNA polymerase-alpha in the yeast Saccharomyces cerevisi

116 inhibitor of and only slowly polymerized by DNA polymerase alpha, indicating that it is a specific p

117 s been carried out on the clamp-loader-clamp-DNA polymerase alpha interactions in Escherichia coli, t

118 In addition, the interaction site for DNA polymerase alpha is composed of two functionally dis

119 hat are involved in chromosomal replication, DNA polymerase alpha is essential for initiation of repl

120 ally limited, it increases dramatically when DNA polymerase alpha is inhibited, indicating that the h

121 Because the fidelity of DNA polymerase alpha is lower than that of the DNA polym

122 Saccharomyces cerevisiae and functions as a DNA polymerase alpha loading factor in Xenopus, but its

123 These data suggest that DNA polymerase alpha may be able to rejoin double-strand

124 We also report that HJs accumulate in a DNA polymerase alpha mutant that lacks Mus81, providing

125 cause of telomere elongation in cdc17/pol1 (DNA polymerase alpha) mutants, we examined telomeric chr

126 Both the cdc13-5 mutation and DNA polymerase alpha mutations (which also exhibit elong

127 Cdc45p is required for recruiting DNA polymerase alpha onto chromatin, and it associates w

128 al independent antibodies specific to either DNA polymerases alpha or beta and then restoring repair

129 oes not stimulate the activity of eukaryotic DNA polymerases alpha or epsilon, or a variety of other

130 CDK2 substrates such as RB, histone H1, and DNA polymerase alpha (p70 subunit) is reduced in the pre

131 Curiously, human DNA polymerase alpha (p70-p180 or p49-p58-p70-p180), whi

132 embly and cell cycle entry into S phase, and DNA polymerase-alpha, PCNA, and ribonucleotide reductase

133 In contrast, DNA polymerase alpha (Pol alpha) accumulates to higher t

134 In fission yeast both DNA polymerase alpha (pol alpha) and delta (pol delta) a

135 analogues and tested them as substrates for DNA polymerase alpha (pol alpha) and Klenow fragment (ex

136 We show aRPA has weakened interaction with DNA polymerase alpha (pol alpha) and that aRPA is not ab

137 We have found that human DNA polymerase alpha (pol alpha) and the Klenow fragment

138 After primase synthesizes a primer that DNA polymerase alpha (pol alpha) can readily elongate, f

139 tal structure of the catalytic core of human DNA polymerase alpha (Pol alpha) in the ternary complex

140 lear DNA replication, proofreading-deficient DNA polymerase alpha (Pol alpha) initiates Okazaki fragm

141 Klenow fragment exo- (Kf exo-), calf thymus DNA polymerase alpha (pol alpha) or human DNA polymerase

142 nks the Cdc45-MCM-GINS (CMG) DNA helicase to DNA polymerase alpha (Pol alpha) within the replisome.

143 with mutagenic bases, we examined how human DNA polymerase alpha (pol alpha), a B family enzyme, and

144 ow the initiator primase-polymerase complex, DNA polymerase alpha (pol alpha), is brought to the orig

145 rying temperature-sensitive alleles of PCNA, DNA polymerase alpha (Pol alpha), or primase showed that

146 is secured by its physical association with DNA polymerase alpha (Pol alpha), which extends the RNA

147 based on the logic that errors made by yeast DNA polymerase alpha (Pol alpha), which initiates Okazak

148 n of several replication proteins, including DNA polymerase alpha (pol alpha), with chromatin.

149 ity is mediated through the Pol12 subunit of DNA polymerase alpha (Pol alpha).

150 The DNA Polymerase alpha (Pol alpha)/primase complex initiat

151 Here, we show that a mutation in DNA polymerase alpha (pol(alpha)) affects Swi6 localizat

152 hich forms a projection, specifically blocks DNA polymerase alpha (Pol-alpha) and Ctf4 recruitment wi

153 s been shown to interact physically with the DNA polymerase-alpha (pol-alpha).primase complex.

154 s-1 reverse transcriptase (HIV RT) and human DNA polymerases alpha (pol alpha), beta (pol beta), gamm

155 abasic lesions on both primase activity and DNA polymerase alpha- (pol alpha) catalyzed elongation o

156 teins that bound to the catalytic subunit of DNA polymerase alpha (Pol1 protein) are encoded by the e

157 ngly, mutants of lagging strand replication, DNA polymerase alpha (pol1-17), DNA primase (pri2-1), an

158 n vivo and in vitro, to the large subunit of DNA polymerase alpha, POL1, requires the carboxyl-proxim

159 nd genetically with the catalytic subunit of DNA polymerase alpha, Pol1.

160 nteracted with both the catalytic subunit of DNA polymerase alpha, Pol1p, and the telomerase RNA-asso

161 nstrate that C-strand fill-in is mediated by DNA polymerase alpha (polalpha) and controlled by cyclin

162 es the RNA primer, which is then extended by DNA polymerase alpha (Polalpha) to synthesize an initiat

163 ome, a 340-kilodalton complex of primase and DNA polymerase alpha (Polalpha), synthesizes chimeric RN

164 ith the principal initiation DNA polymerase, DNA polymerase alpha (Polalpha).

165 required to maintain steady-state levels of DNA polymerase-alpha (polalpha).

166 at telomeres earlier than the lagging strand DNA polymerases alpha (Polalpha) and delta (Poldelta).

167 NA-DNA hybrid primers synthesized by primase-DNA polymerase alpha (Prim-Pol alpha) are needed to star

168 eplication in vitro at low concentrations of DNA polymerase-alpha primase (Pol-primase), and the p58

169 r (AAF) complex, stimulating the activity of DNA polymerase-alpha primase, the only enzyme known to i

170 can only occur during G1; the recruitment of DNA polymerase alpha-primase (pol alpha) to chromatin.

171 The recruitment of DNA polymerase alpha-primase (pol-prim) is a crucial ste

172 DNA polymerase alpha-primase (pol-prim) is a heterotetra

173 DNA polymerase alpha-primase (pol-prim) plays a central

174 DNA polymerase alpha-primase (Pol-prim) plays an essenti

175 cellular proteins, replication protein A and DNA polymerase alpha-primase (pol-prim), constituting th

176 The B-subunit (p70/Pol12p) of the DNA polymerase alpha-primase (Polalpha-primase) complex

177 All other replication proteins, including DNA polymerase alpha-primase (polalpha-primase), are der

178 show that phosphorylation of purified human DNA polymerase alpha-primase by purified cyclin A/cdk2 i

179 The DNA polymerase alpha-primase complex forms an essential

180 different cellular replication proteins, the DNA polymerase alpha-primase complex, the replication pr

181 tro primer extension assay and the mammalian DNA polymerase alpha-primase complex, we have observed a

182 uman genome is accomplished primarily by the DNA polymerase alpha-primase complex, which makes the RN

183 iv gene, div-1, encodes the B subunit of the DNA polymerase alpha-primase complex.

184 1 protein has also been shown to bind to the DNA polymerase alpha-primase complex.

185 activities of DNA polymerase epsilon and the DNA polymerase alpha-primase complex.

186 Phosphopeptide maps of the p68 subunit of DNA polymerase alpha-primase from human cells, synchroni

187 nuclease activity that copurified with yeast DNA polymerase alpha-primase in a multiprotein complex.

188 lts suggest that the replication activity of DNA polymerase alpha-primase in human cells is regulated

189 DNA polymerase alpha-primase is known to be phosphorylat

190 Consistent with this, the ability of DNA polymerase alpha-primase isolated from synchronized

191 First, DNA polymerase alpha-primase synthesizes the primed site

192 ding to the viral E2 protein and to cellular DNA polymerase alpha-primase were all unaffected in the

193 Host enzymes, notably DNA polymerase alpha-primase, DNA ligase I, and topoisom

194 for dimerization and for binding to DNA and DNA polymerase alpha-primase, provides an effective mean

195 on initiation following the requirements for DNA polymerase alpha-primase, replication factor C, and

196 to the BPV1 origin of replication, including DNA polymerase alpha-primase, replication protein A (RPA

197 ty but retained the ability to interact with DNA polymerase alpha-primase, suggesting that the mutant

198 interacted physically and functionally with DNA polymerase alpha-primase.

199 the six Mcm proteins and the p180 subunit of DNA polymerase alpha-primase.

200 d DNA synthesis, we found no requirement for DNA polymerase alpha-primase.

201 terminal 323 residues of the p180 subunit of DNA polymerase alpha-primase.

202 DNA polymerase-alpha-primase may be isolated from pea sh

203 en, a marker for replication foci containing DNA polymerase-alpha.primase and RPA.

204 sory factor (AAF) stimulates the activity of DNA polymerase-alpha.primase, the only enzyme known to i

205 lex DNA, inhibits DNA synthesis catalyzed by DNA polymerase alpha/primase (pol alpha).

206 re we report that p12(DOC-1) associates with DNA polymerase alpha/primase (pol-alpha:primase) in vitr

207 lular proteins, replication protein A (RPA), DNA polymerase alpha/primase (pol/prim) and topoisomeras

208 ke murine DOC-1, human DOC-1 associates with DNA polymerase alpha/primase and mediates the phosphoryl

209 n part due an ability of p12 to bind to both DNA polymerase alpha/primase and to cyclin-dependent kin

210 complex was found to copurify with the yeast DNA polymerase alpha/primase complex, further supporting

211 proteins, including the Mcm2-7 helicase and DNA polymerase alpha/primase complexes.

212 Purified DNA polymerase alpha/primase was capable of catalyzing s

213 acent short region of DNA are synthesized by DNA polymerase alpha/primase.

214 trast, the distribution of XMCM3, XORC2, and DNA polymerase alpha, proteins required for the initiati

215 d rolling circles, including SV40 T antigen, DNA polymerase alpha, replication protein A (RPA) and RF

216 ells along with E2F1 and the target promoter DNA polymerase alpha, repression of transcription was ob

217 xtends the available data base of eukaryotic DNA polymerase alpha sequences, and suggests new amino a

218 PS AS ODNs against DNA polymerase alpha showed less activity than that for

219 DNA polymerase alpha showed the most discontinuous DNA s

220 netically with POL1 and CTF4, which encode a DNA Polymerase alpha subunit and an associated protein,

221 e ability of RPA to increase processivity of DNA polymerase alpha, suggesting that this activity of R

222 However, only PrimPol, DNA polymerase alpha, telomerase, and the mitochondrial

223 Mutations in Rif2 and DNA polymerase alpha that cause increased telomere elong

224 Here, we show that the RPC associates with DNA polymerase alpha that primes each Okazaki fragment d

225 subunit (60 kD) of DNA primase, the part of DNA polymerase alpha that synthesizes RNA primers during

226 A primase synthesizes short RNA primers that DNA polymerase alpha then elongates during the initiatio

227 proliferating cell nuclear antigen, but not DNA polymerase alpha, to the nascent replication fork.

228 gistic activation of the p68 subunit gene of DNA polymerase alpha together with E2F3, again dependent

229 Decreased DNA polymerase alpha was followed by checkpoint arrest d

230 st, bypass replication efficiency with yeast DNA polymerase alpha was no more than 1 percent.

231 However, no repression of DNA polymerase alpha was seen if the cells expressed a n

232 Discontinuous DNA synthesis by DNA polymerase alpha was seen with substrates containing

233 e kinase activity, no significant changes in DNA polymerase alpha were observed in factor-deprived NF

234 Two other TATA-less promoters, cyclin D3 and DNA polymerase alpha, were also found to be repressed by

235 vity, as well as an association of PARP with DNA polymerase alpha, within 12-24 h of exposure to indu