ライフサイエンスコーパス: DNA polymerase delta

1 enes encoding the Pol3 and Cdc27 subunits of DNA polymerase delta.

2 to mutations affecting ORC, Mcm proteins, or DNA polymerase delta.

3 lap endonuclease 1 (FEN1), DNA ligase I, and DNA polymerase delta.

4 topoisomerase III, replication protein A and DNA polymerase delta.

5 reconstitute fully active recombinant human DNA polymerase delta.

6 6, and p12 have been assigned as subunits of DNA polymerase delta.

7 isiae, POL3 encodes the catalytic subunit of DNA polymerase delta.

8 rier for the strand displacement activity of DNA polymerase delta.

9 utation in the 3'-->5' exonuclease domain of DNA polymerase delta.

10 , a temperature-sensitive mutation affecting DNA polymerase delta.

11 replication protein A (RPA), RFC, PCNA, and DNA polymerase delta.

12 (PCNA), a requisite processivity factor for DNA polymerase delta.

13 at supported PCNA-dependent DNA synthesis by DNA polymerase delta.

14 p50 interacts with the catalytic subunit of DNA polymerase delta.

15 art on the 3'-to-5' proofreading activity of DNA polymerase delta.

16 in other genes, including the gene encoding DNA polymerase delta.

17 (13D5) inhibited the activity of calf thymus DNA polymerase delta.

18 similarity to the small subunit of mammalian DNA polymerase delta.

19 NA polymerase III, the analogue of mammalian DNA polymerase delta.

20 ar antigen (PCNA), a processivity factor for DNA polymerase delta.

21 l peptides of the catalytic subunit of human DNA polymerase delta.

22 merase epsilon as compared to lagging-strand DNA polymerase delta.

23 0 (RPA70), replication factor C1 (RFC1), and DNA polymerase delta.

24 ssDNA template and inhibits DNA synthesis by DNA polymerase delta.

25 e first evidence for the targeting of PP1 to DNA polymerase delta.

26 coimmunoprecipitate with Hys2, a subunit of DNA polymerase delta.

27 ncer-cell DNA replication and interacts with DNA polymerase delta.

28 flap by the strand displacement activity of DNA polymerase delta.

29 for cells with defects in the lagging-strand DNA polymerase delta.

30 , Rad27p, or the proofreading exonuclease of DNA polymerase delta.

31 d27p, or the DNA proofreading exonuclease of DNA polymerase delta.

32 late translesion synthesis by Pol zeta or by DNA polymerase delta.

33 tigen (PCNA), replication factor C (RFC) and DNA polymerase delta.

34 conserved region of the coding sequence for DNA polymerase delta, a replicative DNA polymerase.

35 trate that GST-pRb fusion protein stimulates DNA polymerase delta activity.

36 at it supports cell viability and stimulates DNA polymerase delta activity.

37 of CRL4(Cdt2), i.e. the direct regulation of DNA polymerase delta, adding to its known functions in t

38 such as DNA polymerase beta and subunits of DNA polymerase delta along with the EBV-encoded DNase BG

39 cts were competent for DNA replication using DNA polymerases delta, alpha, and possibly epsilon, with

40 Calf thymus DNA polymerase delta also fully restored mismatch repair

41 tion depends on the proofreading activity of DNA polymerase-delta, although the repair proteins Msh2,

42 The repair reaction displays specificity for DNA polymerase delta, an effect that presumably reflects

43 POL3 encodes the catalytic subunit of DNA polymerase delta, an essential DNA polymerase involv

44 ein required for processive DNA synthesis by DNA polymerase delta and epsilon.

45 A is its role as the processivity factor for DNA polymerase delta and epsilon.

46 and the mutant pcna interacts normally with DNA polymerase delta and epsilon.

47 ding motif, includes a sequence conserved in DNA polymerase delta and implicated in its PCNA binding.

48 teins which bound to PCNA-Sepharose included DNA polymerase delta and straightepsilon, PCNA, the 37 a

49 udding yeast) that impede the interaction of DNA polymerase delta and the 5'-flap endonuclease Rad27/

50 etween cdc19+ and genes encoding subunits of DNA polymerase delta and the replication initiator cdc18

51 NA-DNA primers to be extended by replicative DNA polymerases delta and .

52 ences within FRA16D by the replicative human DNA polymerases delta and alpha, and with human cell-fre

53 of defects in the exonuclease activities of DNA polymerases delta and epsilon (conferred by the pol3

54 yotes, processive DNA synthesis catalyzed by DNA polymerases delta and epsilon (pol delta and epsilon

55 east, we confirm the strand specificities of DNA polymerases delta and epsilon and show that the PCNA

56 Both PCNA-associated DNA polymerases delta and epsilon are important for gene

57 The DNA polymerases delta and epsilon are the major replicat

58 The resulting excision gap is filled in by DNA polymerases delta and epsilon as revealed by the 'ph

59 3'-->5' exonuclease activity of replicative DNA polymerases delta and epsilon but did not enhance th

60 PCNA is an accessory factor of DNA polymerases delta and epsilon that is required for D

61 proofreading) by the exonuclease activity of DNA polymerases delta and epsilon, and post-replication

62 ar antigen (PCNA), a processivity factor for DNA polymerases delta and epsilon, is essential for both

63 ar antigen (PCNA), a processivity factor for DNA polymerases delta and epsilon, is involved in DNA re

64 esis catalyzed by the eukaryotic replicative DNA polymerases delta and epsilon.

65 filling of model 30 nt gaps by both purified DNA polymerases delta and epsilon.

66 ar antigen (PCNA)-dependent DNA synthesis by DNA polymerases delta and epsilon.

67 CNA) are processivity factors for eukaryotic DNA polymerases delta and epsilon.

68 cation and repair as an auxiliary factor for DNA polymerases delta and epsilon.

69 f an AP site by the combined action of yeast DNA polymerases delta and zeta.

70 s with somatic mutations in two of the major DNA polymerases, delta and epsilon, that replicate the g

71 trinsic exonuclease domains of the two major DNA polymerases, delta and epsilon.

72 This sequence was found to be present on DNA polymerase delta, and a peptide conforming to this s

73 r antigen clamp loader replication factor C, DNA polymerase delta, and DNA ligase I in the absence of

74 factor C, PCNA, flap endonuclease 1 (FEN1), DNA polymerase delta, and DNA ligase I.

75 The activity and processivity of native DNA polymerase delta are markedly stimulated by PCNA whe

76 involved in DNA synthesis, such as PCNA and DNA polymerase delta, are concentrated in perinucleolar

77 argue that DNA polymerase zeta, rather than DNA polymerase delta as previously suggested, is respons

78 Following this, DNA polymerase delta assembles with PCNA for processive

79 red for PCNA-dependent BER (AP endonuclease, DNA polymerases delta, beta and DNA ligase, and FEN1 end

80 s arrest is not due to 5-FU lesions blocking DNA polymerase delta but instead depends, in part, on th

81 ed capacity in enhancing the processivity of DNA polymerase delta but showed no deficiency in stimula

82 merase, and the most pure fraction contained DNA polymerase delta but was free of detectable DNA poly

83 PP1 was shown to associate with human DNA polymerase delta by affinity chromatography and coim

84 re isolated with highly purified calf thymus DNA polymerase delta by conventional chromatography.

85 DNA polymerase delta can support leading-strand synthesi

86 The DNA polymerase delta catalytic subunit gene (POLD1) was

87 or the replicative RFC in the PCNA-dependent DNA polymerase delta-catalyzed DNA replication reaction.

88 unwound large (CTG)(n) hairpins and promoted DNA polymerase delta-catalyzed DNA synthesis using a (CT

89 to its native counterpart in (i) supporting DNA polymerase delta-catalyzed PCNA-dependent DNA chain

90 m DNA, making the DNA template available for DNA polymerase delta-catalyzed resynthesis.

91 Likewise, expression of the intracellular DNA polymerase delta cofactor/proliferating-cell nuclear

92 h p50 and abet dynamic relocalization of the DNA polymerase delta complexes within the nucleus.

93 DNA polymerase delta consists of four subunits, one of w

94 In this report, we show that human DNA polymerase delta (delta) is capable of robust DNA sy

95 The four-subunit DNA polymerase delta displayed a specific activity compa

96 cteristic for authentic RF-C: stimulation of DNA polymerase delta DNA synthesis on singly primed sing

97 Recombinant DNA polymerase delta efficiently replicated singly prime

98 BER factors flap endonuclease 1 (FEN-1) and DNA polymerase delta/epsilon was also observed, suggesti

99 ovement through undamaged DNA slows down and DNA polymerase delta fails to associate with replicating

100 inding and catalysis by DNA polymerase beta, DNA polymerase delta, FEN1, and DNA ligase I.

101 o of the several sites involved in tethering DNA polymerase delta for processive DNA synthesis during

102 inity purification of recombinant p50 and of DNA polymerase delta from calf thymus or HeLa extracts.

103 er, rigorous purification of human or bovine DNA polymerase delta from natural sources has usually yi

104 ing of Okazaki fragments) and POL3 (encoding DNA polymerase delta) genes on the stability of a minisa

105 The catalytic subunit of human DNA polymerase delta has been overexpressed in insect ce

106 tated with human p50, as well as calf thymus DNA polymerase delta heterodimer, but not with p125 alon

107 ome maintenance complex component (MCM7) and DNA polymerase delta hindering replication fork progress

108 d to enhance the processivity of calf thymus DNA polymerase delta holoenzyme similar to calf thymus P

109 ion of primed DNA templates catalyzed by the DNA polymerase delta holoenzyme.

110 rocessive elongation of DNA catalyzed by the DNA polymerase delta holoenzyme.

111 T2AA abolished interaction of PCNA and DNA polymerase delta in cellular chromatin.

112 serve as the lagging strand replicase, like DNA polymerase delta in eukaryotes, but instead function

113 th pol3+, which encodes the large subunit of DNA polymerase delta in fission yeast, and the Cdc1 prot

114 have co-expressed the two subunits of human DNA polymerase delta in insect cells.

115 hese observations, we infer a requirement of DNA polymerase delta in post-replicative bypass of UV-da

116 antly alter the ability of PCNA to stimulate DNA polymerase delta in the absence of RFC but substanti

117 we have evaluated possible participation of DNA polymerase delta in the excision step of repair.

118 he coding region of the catalytic subunit of DNA polymerase delta in XP variant cell lines revealed t

119 antigen (PCNA), the processivity factor for DNA polymerase delta, in mature cells of Nicotiana benth

120 ed levels of Pol3p, the catalytic subunit of DNA polymerase delta, induce instability at these same s

121 DNA polymerase delta is a heterodimer consisting of a ca

122 se results suggest that the small subunit of DNA polymerase delta is essential for functional interac

123 roliferating cell nuclear antigen (PCNA) and DNA polymerase delta is essential for processive DNA syn

124 POLD1 gene encoding the catalytic subunit of DNA polymerase delta is G/C-rich and does not contain a

125 de that both the quantity and the quality of DNA polymerase delta is important in ensuring genome sta

126 However, DNA polymerase delta is involved indirectly in mutagenes

127 establish that the interaction of PCNA with DNA polymerase delta is mediated through the small subun

128 DNA polymerase delta is required for lagging-strand synt

129 h repair to the depleted extract, indicating DNA polymerase delta is required for mismatch repair in

130 Under this condition, DNA polymerase delta is semidisabled and causes a delay

131 The strand displacement activity of DNA polymerase delta is strongly stimulated by its inter

132 Eukaryotic DNA polymerase delta is thought to consist of three (bud

133 DNA polymerase delta is usually isolated as a heterodime

134 at degradation of p12, the fourth subunit of DNA polymerase delta, is critical for inhibiting fork pr

135 rast to the native heterodimeric calf thymus DNA polymerase delta, is not responsive to stimulation b

136 pared with those of the native heterodimeric DNA polymerase delta isolated from fetal calf thymus, an

137 root of the clade containing all eukaryotic DNA polymerase delta members but that this clade does no

138 in the "proofreading" exonuclease domain of DNA polymerase delta) mutations were synthetically letha

139 action cannot be promoted by the replicative DNA polymerase delta or by other TLS polymerases such as

140 n in this process have suggested that either DNA polymerase delta or DNA polymerase epsilon may be in

141 can facilitate DNA replication by tethering DNA polymerase delta or DNA polymerase epsilon to the DN

142 nuclear antigen onto DNA, where it recruits DNA polymerase delta or epsilon to the primer terminus a

143 achieved by including highly purified human DNA polymerase delta or epsilon, PCNA, RFC, and DNA liga

144 endent processive DNA synthesis catalyzed by DNA polymerase delta or epsilon.

145 pass of the abasic site was also detected by DNA polymerase delta or eta (Rad30).

146 (PCNA) is a processivity factor required for DNA polymerase delta (or epsilon)-catalyzed DNA synthesi

147 The catalytic subunit of DNA polymerase delta (p125) contains a LXCXE motif.

148 nstrated that the catalytic subunit of human DNA polymerase delta (p125) expressed in baculovirus-inf

149 gh small but detectable levels were seen for DNA polymerases delta (+PCNA) and straightepsilon (- PCN

150 In addition to DNA polymerase delta, PCNA, RFC, and RPA, 5'-directed re

151 model whereby the high-fidelity replicative DNA polymerase delta performs recombination-associated D

152 strain carrying a thermosensitive allele of DNA polymerase delta (pol delta ts03).

153 DNA polymerase delta (Pol delta) and DNA polymerase epsi

154 The formation of a complex between DNA polymerase delta (pol delta) and its sliding clamp,

155 ed for processive DNA synthesis catalyzed by DNA polymerase delta (pol delta) and polymerase epsilon.

156 ectrophoresis, is formed between calf thymus DNA polymerase delta (pol delta) and synthetic oligonucl

157 aturation requires the coordinated action of DNA polymerase delta (Pol delta) and the FLAP endonuclea

158 itiated by an RNA/DNA primer and extended by DNA polymerase delta (pol delta) and the replication cla

159 DNA Polymerase delta (Pol delta) and the Werner syndrome

160 ability to promote DNA synthesis by purified DNA polymerase delta (pol delta) both on unmodified temp

161 -ct mutants, defective for components of the DNA polymerase delta (Pol delta) complex.

162 Primer extension by DNA polymerase delta (pol delta) displaces the downstrea

163 DNA helicase that partially co-purifies with DNA polymerase delta (pol delta) from fetal bovine thymu

164 DNA polymerase delta (Pol delta) from Saccharomyces cere

165 the p12 subunit in the function of the human DNA polymerase delta (Pol delta) holoenzyme by comparing

166 we constructed a series of recombinant human DNA polymerase delta (Pol delta) in which one or two of

167 DNA polymerase delta (pol delta) is a high fidelity euka

168 DNA polymerase delta (Pol delta) is a key enzyme in mamm

169 Eukaryotic DNA polymerase delta (pol delta) is a member of the B fa

170 replication and in several types of repair, DNA polymerase delta (pol delta) is assisted by replicat

171 Mammalian DNA polymerase delta (Pol delta) is believed to replicat

172 Importantly, we found that DNA polymerase delta (Pol delta) is critical for MMEJ, i

173 DNA polymerase delta (Pol delta) is one of the major rep

174 DNA polymerase delta (pol delta) is one of the two main

175 In eukaryotes, DNA polymerase delta (pol delta) is responsible for repl

176 DNA polymerase delta (Pol delta) is thought to catalyze

177 DNA polymerase delta (Pol delta) isolated from Schizosac

178 (PCNA) promotes DNA synthesis by calf thymus DNA polymerase delta (pol delta) past several chemically

179 DNA polymerase delta (Pol delta) plays a central role in

180 DNA polymerase delta (pol delta) plays an essential role

181 Eukaryotic DNA polymerase delta (Pol delta) plays an essential role

182 ouble mutant allele, which causes defects in DNA polymerase delta (Pol delta) proofreading (pol3-01)

183 of proliferating cell nuclear antigen, yeast DNA polymerase delta (Pol delta) replicated DNA at a rat

184 telomere damage to establish predominance of DNA polymerase delta (Pol delta) through its POLD3 subun

185 dihydroguanine (8-oxoG) by fetal calf thymus DNA polymerase delta (pol delta) was examined by steady-

186 at interacts with the small subunit (p50) of DNA polymerase delta (pol delta) was identified in a two

187 analysis of the 3'-->5' exonuclease of yeast DNA polymerase delta (Pol delta) we have discerned addit

188 dination of strand displacement synthesis by DNA polymerase delta (Pol delta) with 5.-flap cutting by

189 ow here that WRN functionally interacts with DNA polymerase delta (pol delta), a eukaryotic polymeras

190 Mammalian DNA polymerase delta (pol delta), a key enzyme of chromo

191 ough tight coordination of the activities of DNA polymerase delta (Pol delta), flap endonuclease 1 (F

192 ed to study complexes formed among mammalian DNA polymerase delta (pol delta), proliferating cell nuc

193 lizing antibodies suggested a role for yeast DNA polymerase delta (Pol delta), RFC and PCNA in LLR re

194 DNA polymerase delta (Pol delta), which plays keys roles

195 an will be more internal errors generated by DNA polymerase delta (Pol delta), which takes over for P

196 iscontinuous strand that takes place in both DNA polymerase delta (Pol delta)- and DNA polymerase (Po

197 he third subunit of Saccharomyces cerevisiae DNA polymerase delta (Pol delta).

198 ify novel proteins that associate with human DNA polymerase delta (pol delta).

199 ous template-primer and purified calf thymus DNA polymerase delta (pol delta).

200 enerated by an asymmetric mutator variant of DNA polymerase delta (Pol delta).

201 '-upstream to a DNA SSB and identified it as DNA polymerase delta (Pol delta).

202 positions of components of the DNA-dependent DNA polymerase delta (pol delta).proliferating cell nucl

203 DNA polymerase delta (Pol delta4) is a heterotetrameric

204 Human DNA polymerase delta (Pol delta4), a key enzyme in chrom

205 The mutator effects of mutations in the DNA polymerase delta (POL3) gene and the recombinational

206 on with three essential replication enzymes: DNA polymerase delta (POLD1), DNA primase (PRIM1), and m

207 Allele-specific mutants of DNA polymerase delta (poldelta) and mutants of Polalpha,

208 Yeast DNA polymerase delta (Poldelta) consists of three subuni

209 Yeast DNA polymerase delta (Poldelta) has three subunits of 12

210 DNA polymerase delta (Poldelta) is a multisubunit polyme

211 DNA polymerase delta (Poldelta) plays an essential role

212 The intolerance of DNA polymerase delta (Poldelta) to incorrect base pairin

213 that encodes Poleta and the Pol32 subunit of DNA polymerase delta (Poldelta).

214 tation (D400A) in the proofreading domain of DNA polymerase delta (poldelta, encoded by the Pold1 gen

215 tigen (PCNA) for functional interaction with DNA polymerases delta (Poldelta) and epsilon (Pol epsilo

216 Defects in DNA polymerases delta (Poldelta) and epsilon (Polepsilon

217 tations in the POLD1 and POLE genes encoding DNA polymerases delta (Poldelta) and varepsilon (Polvare

218 The crystal structure of the human DNA polymerase delta processivity factor PCNA (prolifera

219 The DNA polymerase delta processivity factor Proliferating C

220 They were able to function as weak DNA polymerase delta processivity factors in vitro, and

221 the Bloom syndrome helicase (BLM) stimulates DNA polymerase delta progression across telomeric G-rich

222 synthesis and progression by the replicative DNA polymerase delta/proliferating cell nuclear antigen/

223 ombination-associated DNA synthesis and that DNA polymerase delta promotes recombination-associated D

224 Finally, a diploid strain with a defect in DNA polymerase delta proofreading exhibits a higher muta

225 The Saccharomyces cerevisiae DNA polymerase delta proofreading exonuclease-defective

226 The contribution of DNA polymerase delta proofreading on error avoidance was

227 ns of 4 to 14 bases to examine the impact of DNA polymerase delta proofreading on mutation avoidance.

228 ion at L604 in the polymerase active site of DNA polymerase delta reduces life span, increases genomi

229 a reaction requiring S. pombe Uve1p, Rad2p, DNA polymerase delta, replication factor C, proliferatin

230 of the catalytic and small subunits of human DNA polymerase delta results in formation of a stable, f

231 the processivity or proofreading activity of DNA polymerase delta shortened hetDNA length or reduced

232 ffects of recombinant p50 on the activity of DNA polymerase delta showed that p50 is able to slightly

233 of DNA ligase III, while those catalyzed by DNA polymerase delta/straightepsilon appeared to be more

234 However, DNA polymerase delta/straightepsilon was also capable of

235 , DNA polymerase zeta, Rev1 protein, and the DNA polymerase delta subunit, Pol32, in the bypass of an

236 air; however, the identity of the subunit of DNA polymerase delta that directly interacts with PCNA h

237 m of yeast Rad51, Rad54, RPA, PCNA, RFC, and DNA polymerase delta that loading of PCNA by RFC targets

238 by heterozygosity for a missense mutation in DNA polymerase delta that reduces its proofreading activ

239 fect in the catalytic subunit of replicative DNA polymerase delta that results in increased rates of

240 nuclear antigen, replication protein A, and DNA polymerase delta that supports Exo1-independent repa

241 determine which of the two subunits of core DNA polymerase delta, the 125-kDa catalytic subunit or t

242 aturation by the PCNA-coordinated actions of DNA polymerase delta, the flap endonuclease FEN1, and DN

243 DNA polymerase delta, the key enzyme for eukaryotic chro

244 epsilon incorporated the analogs showed that DNA polymerase delta, the most sensitive of the DNA poly

245 strand displacement synthesis carried out by DNA polymerase delta, the PCNA clamp, its loader RFC, an

246 Instead, Srs2 mainly functioned with DNA polymerase delta to block expansions.

247 In results presented here, Pif1 promoted DNA polymerase delta to displace strands that achieve a

248 the ability of the p125 catalytic subunit of DNA polymerase delta to engage in protein-protein intera

249 se delta that loading of PCNA by RFC targets DNA polymerase delta to the D loop formed by Rad51 prote

250 ta1N, proliferating cell nuclear antigen and DNA polymerase delta was found to repair substrates cont

251 The fidelity of Schizosaccharomyces pombe DNA polymerase delta was measured in the presence or abs

252 Mammalian DNA polymerase delta was originally characterized as a t

253 DNA polymerase delta was shown to extend an upstream fra

254 To reconstitute an intact DNA polymerase delta, we have constructed recombinant ba

255 ability of ATM to stimulate DNA synthesis by DNA polymerase delta, which is implicated in both DNA re

256 DNA polymerase delta, whose catalytic subunit is encoded

257 e SDSA pathway using Rad51, Rad54, RPA, RFC, DNA Polymerase delta with different forms of PCNA.

258 t is essential for functional interaction of DNA polymerase delta with PCNA and for highly processive