ライフサイエンスコーパス: DNA polymerase epsilon

1 1, Mms19 and Cdc20 (the catalytic subunit of DNA polymerase-epsilon).

2 markedly enhances the synthetic activity of DNA polymerase epsilon.

3 interacts with the DNA replication protein, DNA polymerase epsilon.

4 of DNA replication via its interaction with DNA polymerase epsilon.

5 editing 3' exonuclease domain of eukaryotic DNA polymerase epsilon.

6 ication proteins including Mcm10, RFC140 and DNA polymerase epsilon 255 kDa subunit in S-phase.

7 Yeast strains with this DNA polymerase epsilon allele have elevated rates of T t

8 cerevisiae POL2 and POL3 genes, which encode DNA polymerase epsilon and delta, respectively, and show

9 h and stimulate the polymerase activities of DNA polymerase epsilon and the DNA polymerase alpha-prim

10 DNA substrate, the combined action of human DNA polymerase epsilon and the human CMG complex leads t

11 lly with mutations affecting the function of DNA polymerase epsilon and the S-phase checkpoint protei

12 lpha) and Ctf4 recruitment without affecting DNA polymerases epsilon and delta or the CMG helicase.

13 This produces a replisome that lacks DNA polymerase epsilon, and although cells are viable, t

14 Since DNA polymerase epsilon appears to be involved in DNA rep

15 repair of mismatches made by leading-strand DNA polymerase epsilon as compared to lagging-strand DNA

16 lex (or Tim alone) is able to associate with DNA polymerase epsilon bound to a 40-/80-mer DNA ligand.

17 to budding yeast Dpb11, which interacts with DNA polymerase epsilon) but not on Rhp9 (similar to budd

18 2 or the removal of the C-terminal domain of DNA polymerase epsilon by trypsin digestion or competiti

19 lude that the 3' --> 5' exonuclease of human DNA polymerase epsilon can remove 3'-terminal F-ara-AMP

20 The human DNA polymerase epsilon catalytic subunit consists of a 1

21 Additionally, maximal rates only occur when DNA polymerase epsilon catalyzes leading-strand synthesi

22 tation experiments revealed that recombinant DNA polymerase epsilon directly interacts with either Ti

23 o the C-terminus of the catalytic subunit of DNA polymerase epsilon (DNA pol epsilon), to a region th

24 stablishing leading-strand synthesis, before DNA polymerase epsilon engagement.

25 In eukaryotes DNA polymerase epsilon (epsilon) synthesises the leading

26 nteracts with Pol2, the catalytic subunit of DNA polymerase epsilon, essential for leading-strand DNA

27 DNA by the 3' --> 5'-exonuclease activity of DNA polymerase epsilon from human leukemia CEM cells.

28 In contrast, DNA polymerase epsilon, functioned with the chromatin re

29 After DNA polymerase epsilon had been incubated with 3'-F-ara-

30 inetic evaluation demonstrated that although DNA polymerase epsilon has a higher affinity for F-ara-A

31 Ps in DNA could be problematic because yeast DNA polymerase epsilon has difficulty bypassing a single

32 After extension of the upstream primer with DNA polymerase epsilon, human DNA ligase I was able to c

33 eracted with Pol2p, the catalytic subunit of DNA polymerase epsilon, in vivo.

34 Consistent with the hypothesis that DNA polymerase epsilon is the leading-strand enzyme, we

35 uggested that either DNA polymerase delta or DNA polymerase epsilon may be involved.

36 r synthesis, we suggest that MDM2 binding to DNA polymerase epsilon might be part of a reconfiguratio

37 ocus, which encodes the catalytic subunit of DNA polymerase epsilon of Arabidopsis thaliana, causes a

38 atin, and it associates with Mcm2p, RPA, and DNA polymerase epsilon only during S phase.

39 rmore, the 3' --> 5' exonuclease activity of DNA polymerase epsilon or wild-type p53 protein was inef

40 ication and reveal a pattern indicating that DNA polymerase epsilon participates in leading-strand DN

41 ombe cdc20+ encodes the catalytic subunit of DNA polymerase epsilon (pol epsilon) and that this enzym

42 DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon) are both required f

43 erance of evidence supports a model in which DNA polymerase epsilon (Pol epsilon) carries out the bul

44 type and exonuclease-deficient four-subunit DNA polymerase epsilon (Pol epsilon) complex from Saccha

45 Genetic evidence suggests that DNA polymerase epsilon (Pol epsilon) has a noncatalytic

46 DNA polymerase epsilon (pol epsilon) has been implicated

47 DNA polymerase epsilon (Pol epsilon) is a multi-subunit

48 DNA polymerase epsilon (pol epsilon) is a multiple subun

49 DNA polymerase epsilon (Pol epsilon) is a replicative DN

50 DNA polymerase epsilon (Pol epsilon) is believed to play

51 accharomyces pombe, the catalytic subunit of DNA polymerase epsilon (Pol epsilon) is encoded by cdc20

52 Saccharomyces cerevisiae DNA polymerase epsilon (pol epsilon) is essential for ch

53 es during DNA replication has suggested that DNA polymerase epsilon (Pol epsilon) may also play a rol

54 Human DNA polymerase epsilon (pol epsilon) normally contains a

55 DNA polymerase epsilon (pol epsilon) was purified to app

56 The replicative DNA polymerase epsilon (Pol epsilon) was shown to activa

57 oliferating cell nuclear antigen (PCNA), and DNA polymerase epsilon (Pol epsilon), are reduced at sta

58 HeLa DNA polymerase epsilon (pol epsilon), possibly involved

59 HeLa DNA polymerase epsilon (pol epsilon), possibly involved

60 ter understand the functions and fidelity of DNA polymerase epsilon (Pol epsilon), we report here on

61 Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) muta

62 he large subunit of Saccharomyces cerevisiae DNA polymerase epsilon, Pol2, comprises two essential fu

63 ted) cancers caused by mutations that impair DNA polymerase epsilon (POLE) proofreading.

64 rom this individual identified a mutation in DNA polymerase epsilon (POLE) that associated with an ul

65 ns in the proofreading exonuclease domain of DNA polymerase epsilon (POLE-exo*) exhibit a novel mutat

66 Unexpectedly, the leading strand DNA polymerase epsilon (Polepsilon) arrived at telomeres

67 Dpb4p is also a subunit of the DNA polymerase epsilon (polepsilon) complex, and Dls1p i

68 DNA polymerase epsilon (Polepsilon) is a large, four-sub

69 DNA polymerase epsilon (Polepsilon) is a multi-subunit p

70 DNA polymerase epsilon (Polepsilon), one of the three ma

71 d substitutions in the exonuclease domain of DNA polymerase epsilon (Polepsilon).

72 e mutations of the POL2 and MSH2 genes, both DNA polymerase epsilon proofreading and mismatch repair

73 atures for defective DNA mismatch repair and DNA polymerase epsilon proofreading deficiency, along wi

74 5) but much greater than the contribution of DNA polymerase epsilon proofreading in longer runs.

75 avoidance was found to be similar to that of DNA polymerase epsilon proofreading in short homonucleot

76 esidues 265-605) that associates with Cdc45, DNA polymerase epsilon, replication protein A, and two r

77 (also known as Raf2/Clr7/Cmc2), or Cdc20, a DNA polymerase epsilon subunit, results in dissociation

78 We constructed a derivative of yeast DNA polymerase epsilon that retains high replication act

79 jacent normal deoxynucleotide was cleaved by DNA polymerase epsilon, the reaction products appeared t

80 art of a reconfiguration process that allows DNA polymerase epsilon to associate with repair/recombin

81 dress this possibility, the ability of human DNA polymerase epsilon to incorporate ribonucleotides wa

82 tch base excision repair in vitro using calf DNA polymerase epsilon to provide strand displacement sy

83 ication by tethering DNA polymerase delta or DNA polymerase epsilon to the DNA template.

84 ers was observed after the addition of fresh DNA polymerase epsilon to the reaction.

85 Escherichia coli stimulated the activity of DNA polymerase epsilon up to 10- and 40-fold, respective

86 that budding yeast Ctf18-RFC associates with DNA polymerase epsilon, via an evolutionarily conserved

87 he excision substrates, we demonstrated that DNA polymerase epsilon was unable to effectively remove

88 lity of CDT1 proteins to form complexes with DNA polymerase epsilon, which functions in DNA replicati

89 ucleoside triphosphate concentrations, yeast DNA polymerase epsilon, which is implicated in leading s

90 with nonorigin DNA with similar kinetics as DNA Polymerase epsilon, which is present at DNA replicat

91 recently that the Mrc1 subunit of RPCs binds DNA polymerase epsilon, which synthesises the leading st

92 how the Cdc45-MCM-GINS helicase is linked to DNA polymerase epsilon, which synthesizes the leading st