ライフサイエンスコーパス: DNA primase

1 biochemical characterisation of an archaeal DNA primase.

2 s within the M. smegmatis dnaG gene encoding DNA primase.

3 involves interactions of these proteins with DNA primase.

4 polymerase requires RNA primers produced by DNA primase.

5 vivo, primers are synthesized on-template by DNA primase.

6 anism and a fold similar to that of archaeal DNA primase.

7 leads to elevated RNA primer synthesis by T7 DNA primase.

8 nt DNA ligase and/or archeal-eukaryotic-type DNA primase.

9 ed by mutations of Schizosaccharomyces pombe DNA primase.

10 nsferases and archaeal homologs of DnaG-type DNA primases.

11 eria and eukaryotes requires the activity of DNA primase, a DNA-dependent RNA polymerase that lays sh

12 ost all organisms depends on the activity of DNA primase, a DNA-dependent RNA polymerase that synthes

13 ory factors, and primosome (DNA helicase and DNA primase activities).

14 e for Mn(II) over Mg(II), suggesting that T7 DNA primase activity modulation when bound to Mn(II) is

15 bound to single-stranded DNA and stimulated DNA primase activity only in the presence of AAF-132.

16 s the N-terminal domain in T7 gp4 contains a DNA primase activity, this function is lost in metazoan

17 A replication elongation by interfering with DNA primase activity.

18 Slow primer release from DNA primase allows the polymerase to engage the complex

19 ll molecule inhibitors of the activity of T7 DNA primase, an ideal model for bacterial primases due t

20 replication system of bacteriophage T7 both DNA primase and DNA helicase activities are contained wi

21 des with that of the archeal-eukaryotic-type DNA primase and genes for prokaryotic Ku homologs form p

22 A replication, BSLF1 and BALF5, encoding EBV DNA primase and polymerase, respectively, were SM depend

23 primase template affinity and stimulate both DNA primase and polymerase-alpha activities in vitro.

24 on of the amino acid sequences of eucaryotic DNA primase and the family X polymerases indicates that

25 nd extension effected by cooperation between DNA primase and the lagging-strand polymerase.

26 nstants and binding mode for interactions of DNA primase and thymidylate synthetase (TS) with high an

27 imPol is a recently discovered DNA-dependent DNA primase and translesion synthesis DNA polymerase fou

28 In contrast, expression of DNA pol alpha, DNA primase, and RPA was down-regulated in PARP-antisens

29 NA polymerase and the zinc-finger domains of DNA primase are involved in the stabilization of the pri

30 A replication, structural data on eukaryotic DNA primase are lacking.

31 DNA primases are enzymes whose continual activity is req

32 DNA primases are essential for the initiation of DNA rep

33 DNA primases are pivotal enzymes in chromosomal DNA repl

34 Replisome DNA primases are responsible for the synthesis of short

35 DNA primases are template-dependent RNA polymerases that

36 examined the effects of Mn(2+) on eukaryotic DNA primase both in the presence and absence of 5 mM Mg(

37 emonstrate that the [4Fe4S] cluster in human DNA primase can make use of this chemistry to coordinate

38 Most DNA primases can be divided into two classes.

39 DNA primases catalyze the synthesis of oligoribonucleoti

40 DNA primases catalyze the synthesis of oligoribonucleoti

41 DNA primases catalyze the synthesis of the oligoribonucl

42 tion and biochemical characterization of the DNA primase complex and its subunits from the archaeon T

43 The T. kodakaraensis DNA primase complex is a heterodimer containing stoichio

44 Supplementation of such reactions with the DNA primase complex supported lagging strand formation a

45 Photo-cross-linking of single-stranded DNA-primase complexes revealed that whereas the isolated

46 The p58 subunit of human DNA primase contains a region, M288-K344, that is homolo

47 We have identified 56-kDa DNA primases defective in primer delivery by screening f

48 to contain DNA polymerases alpha and delta, DNA primase, DNA helicase, DNA ligase, and topoisomerase

49 Bacterial DNA primase DnaG synthesizes RNA primers required for ch

50 The mechanism of action of B. anthracis DNA primase (DnaG(BA)) may be described in several disti

51 of unknown function the structural genes for DNA primase (dnaG) and the major sigma factor of RNA pol

52 n be reversed by over-expression of the host DNA primase, DnaG.

53 A novel peptide from the DNA primase, DNAP(211-223), was also found.

54 T7 DNA primase does not catalyze extension synthesis by a Z

55 The gene for the DNA primase encoded by Salmonella typhimurium bacterioph

56 DNA primase facilitates binding of DNA helicase to ssDNA

57 We have purified to near homogeneity a DNA primase from a mitochondrial fraction of the trypano

58 e mechanistic characteristics of recombinant DNA primase from Bacillus anthracis.

59 ars to develop a better understanding of how DNA primase from herpes simplex virus I catalyzes primer

60 ity and mechanism of NTP misincorporation by DNA primase from herpes simplex virus-1.

61 the identification and characterisation of a DNA primase from the thermophilic methanogenic archaeon

62 m of primase function and are applicable for DNA primases from other species.

63 nuclear protein (germinal center-associated DNA primase) (GANP), CD74, CD22, NF-kappaB, elongation f

64 We describe here a Trypanosoma brucei DNA primase gene, PRI1, that encodes a 70-kDa protein th

65 DNA primase harboring lysine at position 69 fails to sta

66 As has not been possible since a kinetoplast DNA primase has not been available.

67 ance and biochemical assays, we show that T7 DNA primase has only a slightly higher affinity for DNA

68 DNA primase has two subunits, Spp1 and Spp2 (S. pombe pr

69 The zinc-binding domain (ZBD) of prokaryotic DNA primases has been postulated to be crucial for recog

70 superior in de novo synthesis compared to T7 DNA primase having a shorter linker.

71 e deaminase, and in DNA replication, such as DNA primase, helicase, type A DNA polymerase, and predic

72 nique long 52 gene (UL52; a component of the DNA primase/helicase complex), bICP4, IEtu2, and the uni

73 63-kDa gene 4 protein of phage T7 is also a DNA primase in that it catalyzes the synthesis of oligon

74 Wild-type T7 DNA primase is 10-fold superior in de novo synthesis com

75 T7 DNA primase is composed of a catalytic RNA polymerase do

76 In the bacteriophage T7 replication system, DNA primase is encoded by gene 4 of the phage.

77 The stabilization of the primer by DNA primase is necessary for DNA polymerase to initiate

78 , a single archaeo-eukaryotic primase (AEP), DNA primase, is required for the initiation and progress

79 ence of dATP, glycerol, and Tris buffer, the DNA primase isolated from Thermococcus kodakaraensis cat

80 Mechanisms by which DNA primase levels might influence the faf-dependent cel

81 T7 DNA primase, like other primases, shares limited homolog

82 essor mutations of the temperature-sensitive DNA primase mutant dnaG2903 have been characterized.

83 results explain functional defects in human DNA primase mutants and provide insights into primosome

84 The DNA primase of bacteriophage T7 has a zinc-binding motif

85 ng mutagenesis of the zinc-binding domain of DNA primase of bacteriophage T7 using a bacterial homolo

86 The 63-kDa gene 4 DNA primase of phage T7 catalyzes the synthesis of oligo

87 nly two of 12 potential priming sites of the DNA primase of the pRN1 replicon, but nearly all these m

88 can now be cited demonstrating how the term 'DNA primase' only describes a very narrow subset of thes

89 required for replication initiation, and the DNA primase-polymerase in eukaryotes is pol alpha.

90 In many prokaryotes, a specific DNA primase/polymerase (PolDom) is required for nonhomol

91 Antibodies against DNA primase precipitated telomerase activity from all th

92 replication, DNA polymerase alpha (pol1-17), DNA primase (pri2-1), and Rad27p (rad27 delta) also grea

93 ation enzymes: DNA polymerase delta (POLD1), DNA primase (PRIM1), and minichromosome 6 (MCM6).

94 DNA primases provide oligoribonucleotides for DNA polyme

95 ymerases, DNA helicase, type B cyclin genes, DNA primases, radiation sensitive genes, repaire related

96 hat besides the single iteron, a neighboring DNA primase recognition element called G site is essenti

97 Bacteriophage T7 DNA primase recognizes 5'-GTC-3' in single-stranded DNA.

98 T7 DNA primase recognizes the sequence 5'-NNGTC-3' via a zi

99 Application of the model to DNA primase revealed a preference in the enzyme's second

100 To identify the catalytic core of the T7 DNA primase, single-point mutations were introduced into

101 Although DNA primase structures are available from archaea and ba

102 initiation, we analyzed mutations of the two DNA primase subunit genes of Schizosaccharomyces pombe,

103 nts, helicases, an RNA-binding protein and a DNA primase subunit.

104 ncentration in determining where calf thymus DNA primase synthesizes a primer on a DNA template was e

105 At a replication fork DNA primase synthesizes oligoribonucleotides that serve

106 DNA primase synthesizes short RNA oligonucleotides that

107 DNA primase synthesizes short RNA primers that are requi

108 Human DNA primase synthesizes short RNA primers that DNA polym

109 Human DNA primase synthesizes short RNA primers that DNA polym

110 The T7 DNA primase synthesizes tetraribonucleotides that prime

111 The discovery of an iron-sulfur cluster in DNA primase that contributes to enzymatic activity provi

112 arity to the small subunit of the eukaryotic DNA primase (the p50 subunit of the polymerase alpha-pri

113 quires the replicative DnaB helicase and the DNA primase, the DnaG gene product.

114 im gene encodes the large subunit (60 kD) of DNA primase, the part of DNA polymerase alpha that synth

115 The ability of T7 DNA primase to catalyze template-directed oligoribonucle

116 uires an oligoribonucleotide, synthesized by DNA primase, to initiate the synthesis of an Okazaki fra

117 alysis revealed that upon binding Mn(II), T7 DNA primase undergoes conformational changes near the me

118 cating NADH-quinone reductase subunit A, and DNA primase were expressed in HLA-B27(+) cells, and thei

119 ssential DNA polymerases alpha and delta and DNA primase were required.

120 Until relatively recently, DNA primases were viewed simply as a class of proteins t

121 t organisms, DNA replication is initiated by DNA primases, which synthesize primers that are elongate

122 transfer, the IncP1 plasmid R751, encodes a DNA primase with low specificity for initiation.

123 trand holoenzyme, and by the presence of the DNA primase with ribonucleoside triphosphates.

124 The inclusion of multiple DNA primases within a whole domain of organisms complica