ライフサイエンスコーパス: DNA primer

1 ted with an increased affinity for telomeric DNA primer.

2 cts of mutations in different regions of the DNA primer.

3 recognized as template when annealed with a DNA primer.

4 n telomerase and a substantial region of the DNA primer.

5 t a primer) or by using the 3' OH group of a DNA primer.

6 ded DNA requires complete removal of the RNA/DNA primer.

7 with hydrogel beads (HBs) bearing barcoding DNA primers.

8 ate (RNA primers) yet binds to the 3' end of DNA primers.

9 that very small RNAs may be bound similar to DNA primers.

10 eric DNA de novo to completely non-telomeric DNA primers.

11 an add telomeric repeats only onto telomeric DNA primers.

12 te amounts of material by amplification from DNA primers.

13 strand, allowing recognition of both RNA and DNA primers.

14 sequences, generating potential plus-strand DNA primers.

15 With a duplex telomeric DNA primer, a single-stranded 3' overhang with a minimum

16 e alpha-primase complex, which makes the RNA-DNA primers accessible to processive DNA pols.

17 template translocates and realigns with the DNA primer after synthesizing each repeat.

18 HIV reverse transcriptase could elongate DNA primers after the removal of chain terminators by th

19 merase mutants were able to extend telomeric DNA primers, albeit with reduced efficiency compared to

20 ese cross-links were shown to be between the DNA primer and (i) a protein moiety of approximately 130

21 a synthetic oligonucleotide consisting of a DNA primer and an RNA template.

22 ic Okazaki fragments are initiated by an RNA/DNA primer and extended by DNA polymerase delta (pol del

23 transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template r

24 n that L14 has a function beyond binding the DNA primer and preventing dissociation during multiple r

25 Complementarity between the DNA primer and RNA template is not required for the prim

26 thesis by eliminating mismatches between the DNA primer and the 5' region of the telomerase RNA templ

27 Using several different DNA primers and acceptor oligonucleotides, we found that

28 ion of the processivity with single-stranded DNA primers and double-stranded primers with 3' tails sh

29 is useful for researchers who want to design DNA primers and probes for analyzing highly variable DNA

30 res (RNA, DNA, and LNA templates and RNA and DNA primers) and two types of 5'-activated nucleotides (

31 non-templated addition to a single-stranded DNA primer; and (iii) templated extension of a 5'-tailed

32 s catalyze the 3'-5'-pyrophosphorolysis of a DNA primer annealed to a DNA template in the presence of

33 lease activity of the enzyme is specific for DNA primers annealed to a template strand and requires a

34 Telomeric DNA primers are bound by telomerase both at the active s

35 lementary to the PBS (R18), but not an 18-nt DNA primer, are used.

36 hybridized to a surface-immobilized array of DNA primers, are determined by sensing the number of nuc

37 ish medaka, which extends the same telomeric DNA primer as human telomerase, was not activated by hum

38 d substrate, and positions the 3'-end of the DNA primer at the active site of the enzyme, providing e

39 Degenerate DNA primers based on N-terminal and CNBr cleavage fragme

40 A endonuclease domain mutations do not block DNA primer binding and thus likely inhibit reverse trans

41 ated at the 3'-end, primed as efficiently as DNA primers but would not support exponential amplificat

42 polymerase greatly preferred to elongate the DNA primer by 650-26,000-fold, thus accounting for the e

43 HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal re

44 logs from the chain-terminated 3'-end of the DNA primer by the 3'-5'-exonuclease activity of Pol gamm

45 The spontaneous release of the completed RNA-DNA primer by the Pol alpha/primase complex simplifies c

46 ass III enzymes that elongated non-telomeric DNA primers by annealing them at alternative sites on th

47 -mers) procedure is based on the assembly of DNA primers by ligation of three or more hexamers taken

48 at a region at the 5'-end is unannealed or a DNA primer can be annealed just adjacent to the 5'-end o

49 duced interaction with the -12 region of the DNA primer can facilitate a step in the catalytic region

50 insic proofreading activity during which the DNA primer chain is transferred between the polymerizati

51 group, nucleophilic attack that extends the DNA primer chain, and elimination of pyrophosphate.

52 nate substitutions through the region of the DNA primer contacted by the RNase H primer grip and into

53 DNA primers containing a single abasic site located six

54 trahymena cis-telomerase RNAs, each having a DNA primer covalently linked to its 3' end.

55 ermore, simultaneous optimization of several DNA primer design criteria may improve overall experimen

56 II intron RT in complex with an RNA template-DNA primer duplex and incoming deoxynucleotide triphosph

57 elicase implicated in the removal of the RNA-DNA primer during Okazaki fragment processing.

58 enzyme is necessary for the synthesis of RNA:DNA primers during DNA replication and, strikingly, we f

59 emoves the inhibitor from the 3' terminus of DNA primers, enabling further primer elongation (excisio

60 uding its ability to recognize and bind to a DNA primer end and load the ring-shaped PCNA onto DNA in

61 RFC binds to a DNA primer end and loads PCNA onto DNA in an ATP-depende

62 DNA primer end recognition and PCNA binding activities,

63 designated a Class I enzyme, only elongated DNA primers ending in telomeric nucleotides.

64 P hydrolysis; (ii) binding preferentially to DNA primer ends; (iii) loading mthPCNA onto singly nicke

65 ing protein; (iii) binding preferentially to DNA primer ends; and (iv) catalytically loading PCNA ont

66 ed CdATP and ara-CTP with high affinity in a DNA primer extending over an oligonucleotide template of

67 In vitro DNA primer extension assays indicated that Cl-F-ara-ATP

68 In vitro DNA primer extension demonstrated that FMdC nucleotides

69 esis that the mechanism involves a switch in DNA primer extension from the cognate template to an alt

70 MP resulted in nearly complete inhibition of DNA primer extension.

71 n competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the r

72 y of a telomerase substrate (single-stranded DNA primer) for the enzyme.

73 DNA primers formed by the archaeal complex can be elonga

74 odakaraensis DNA polymerase (Pol) B, whereas DNA primers formed by the p41 catalytic subunit alone we

75 egion, we measured transfer of an elongating DNA primer from a donor DNA to an acceptor DNA.

76 It involves transferring a growing DNA primer from one genomic RNA template in the virus to

77 that the template's 3'-hydroxyl served as a "DNA primer" from which primase elongated to create the o

78 Finally, contact with a DNA primer hybridized to an isogenic RNA or DNA template

79 nucleotide addition to a blunt-ended duplex DNA primer; (ii) non-templated addition to a single-stra

80 a dCMP residue from dCTP to the 3' end of a DNA primer in a template-dependent reaction.

81 otides at the 3' end of the AZTMP-terminated DNA primer in complex with AZT-resistant RT, but not wil

82 on-Crick base pairs between RNA template and DNA primer increases from zero to five.

83 ki fragment and displaces the downstream RNA/DNA primer into a flap removed by nuclease cleavage.

84 Each contains an initiator RNA/DNA primer (iRNA/DNA), which is converted into a 5'-flap

85 irections is achieved, however, when a 6-mer DNA primer is annealed to the primase recognition site o

86 RNA pseudoknot region and the 5' end of the DNA primer is approximately 33 A.

87 ing domain of telomerase RNA and a telomeric DNA primer is normally a characteristic of elongation of

88 C complex onto the 3'-end of the nascent RNA-DNA primer is the last step required for the establishme

89 hich adds telomere repeats to a biotinylated DNA primer is the source of telomerase.

90 for addition of a single ribonucleotide to a DNA primer, Klenow fragment does not efficiently synthes

91 DI mass spectral studies and the efficacy of DNA primers made with the new support in PCR amplificati

92 bled containing enzyme, DNA template (RT20), DNA primer molecule (P12), and the necessary dNTPs (one

93 initial proton abstraction from the terminal DNA primer O3'H group, nucleophilic attack that extends

94 observed with an RNA PPT primer than with a DNA primer of corresponding sequence and with wild-type

95 rporates ribonucleotide triphosphates into a DNA primer offers a plausible enzymatic pathway for the

96 investigated the effects of mutations in the DNA primer on overall binding and polymerization by yeas

97 the 5'-end primer contained either a 5'-OH (DNA primer) or a 5'-triphosphate (RNA primer) group.

98 nd drives the extrusion of the 5'-end of the DNA primer out of the enzyme complex.

99 gh further polymerase-dependent extension of DNA primers past 6-MI template bases is significantly in

100 es consisted of a 50 nucleotide template and DNA primers ranging from 23 to 43 nucleotides.

101 The rate constant for DNA primer realignment reveals this step is not rate lim

102 When a DNA primer recessed on an RNA template had a 3' unanneal

103 ions centered around the -12 position of the DNA primer reduced overall binding affinity but dramatic

104 Downstream DNA primers, RNA primers, and small 5'-flaps were effici

105 Ts exhibit a normal dissociation rate from a DNA primer-RNA template while paused during synthesis.

106 DNA primer sets, labeled with two fluorescent dyes to ex

107 pattern generated by telomerase extension of DNA primers shifted in response to changes in dGTP conce

108 Mass spectral analysis of extended DNA primers showed >/=95% incorporation of dCTP > dATP o

109 quantitative polymerase chain reaction using DNA primers specific for the H-2Kb gene, a sequence not

110 rphic DNA (RAPD) analysis, sequence-specific DNA primer (SSDP) analysis, and polymorphic microsatelli

111 ow concentrations of the latter two RTs, the DNA primer stalled when it encountered the 5'-end of the

112 V-1) reverse transcriptase (RT) contacts the DNA primer strand and positions the template strand near

113 rip domain, which contacts and positions the DNA primer strand near the RNase H active site.

114 1 strand transfers, including the obligatory DNA primer strand transfers as well as recombinational c

115 incorporates nucleoside triphosphates onto a DNA primer strand, filling DNA gaps in annealed breaks.

116 isting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis a

117 NTP) substrates onto the 3' end of a growing DNA primer strand.

118 es to an acceptor molecule positioned on the DNA primer strand.

119 and one catalyzes its condensation with the DNA primer strand.

120 and of identical composition, and it prefers DNA primer strands containing a short 3'-ribonucleotide

121 d in comparing the DNA-TNA primer to the all-DNA primer, suggesting that few primer-enzyme contacts a

122 f 3 mutants and their ability to use an 8 nt DNA primer, suggests that motif 3 facilitates realignmen

123 at Pif1p inhibits telomerase by dissociating DNA primer-telomerase RNA interactions.

124 of a high fidelity DNA polymerase I bound to DNA primer-template caught in the act of binding a misma

125 poration for all four tNTPs and dNTPs from a DNA primer-template complex and carried out parallel exp

126 cus gorgonarius polymerase in complex with a DNA primer-template containing uracil in the single-stra

127 ially degrades AT-rich compared with GC-rich DNA primer-template in the absence of DNA synthesis.

128 d slower than the values for DNA/RNA and DNA/DNA primer-template substrates, respectively, while the

129 A "minimal" DNA primer-template system, consisting of an 80-mer temp

130 lso have a 3'-phosphatase activity on an all-DNA primer-template that yields a 3'-OH DNA end.

131 main has a 3'-phosphatase activity on an all-DNA primer-template, signifying that the phosphomonoeste

132 Using a minicircle DNA primer-template, the wild-type catalytic subunit of

133 ribonucleotides or deoxyribonucleotides to a DNA primer-template, with rNTPs being the preferred subs

134 polymerase beta (beta pol) complexed with a DNA primer-template.

135 pable of adding up to 4 ribonucleotides to a DNA primer-template.

136 i) templated extension of a 5'-tailed duplex DNA primer-template.

137 rent DNA structures: single-stranded DNA (ss-DNA), primer-template DNA (pt-DNA), and blunt-end double

138 DNA primer/template (p/t) constructs are used as models

139 ts indicate that KF is able to dimerize on a DNA primer/template and that dimerization is favored whe

140 (pol II-) were used as model enzymes with a DNA primer/template complex (12/16-mer) to examine the k

141 olymerase beta (Pol beta), using a synthetic DNA primer/template containing 2-aminopurine (2-AP) at t

142 freading polymerase, the nascent 3' end of a DNA primer/template has two possible fates.

143 The tC donor was incorporated within a model DNA primer/template in place of a normal base, adjacent

144 h-throughput assays, a double-stranded oligo DNA primer/template was used as a substrate.

145 hilus) DNA polymerase I large fragments with DNA primer templates bound productively at the polymeras

146 aired mutD5 strain to polymerize from M13mp2 DNA primer-templates containing a terminal T(template).C

147 w fragment of DNA polymerase I and synthetic DNA primer-templates containing extrahelical bases at de

148 a coli DNA polymerase I (Klenow fragment) to DNA primer-templates modified with an AAF or AF adduct.

149 Comparing RNA primer-templates and DNA primer-templates of identical sequence showed that h

150 catalyzed elongation of exogenously supplied DNA primer-templates showed that abasic lesions strongly

151 ual amino acid side chains to the binding of DNA primer-templates to the 3'-5' exonuclease site of th

152 ntrinsic proofreading activity interact with DNA primer/templates in two distinct modes, correspondin

153 Gapped DNA primer/templates were extended but not significantly

154 an DNA polymerases, p53 protein, and defined DNA primer/templates, we demonstrated that the wild-type

155 Human DNA polymerase alpha extended DNA primers terminated by CdA monophosphate (CdAMP) at i

156 sted for their ability to unblock and extend DNA primers terminated with AZT and other NRTIs, when co

157 The partitioning of the DNA primer terminus between the polymerase and 3'-5' exo

158 rectly paired ribonucleotide is added to the DNA primer terminus more rapidly than the corresponding

159 lex would promote helix melting ahead of the DNA primer terminus to create a small gap of nondisplace

160 ry cuts, about eight nucleotides in from the DNA primer terminus.

161 method employs Cy5-dATP incorporation into a DNA primer that has been prelabeled with a reference flu

162 Okazaki fragments contain an initiator RNA/DNA primer that must be removed before the fragments are

163 verse transcriptase does not efficiently use DNA primers that are base-paired to internal positions i

164 telomeric 3' termini in vitro using chimeric DNA primers that carried one repeat of a telomeric seque

165 imase and pol alpha synthesize composite RNA-DNA primers that initiate the leading and lagging DNA st

166 bridized to its extruded 5' end and allows a DNA primer to anneal and be extended by the DNA polymera

167 inding of the 3' end of the AZTMP-terminated DNA primer to reverse transcriptase is involved in the m

168 e alpha (Polalpha), synthesizes chimeric RNA-DNA primers to be extended by replicative DNA polymerase

169 endent RNase H2-mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent am

170 onformational dynamics of the intramolecular DNA primer transfer during the processive replicative ac

171 To make relaxed circular DNA, primer translocation must occur, resulting in the t

172 ted of a 142 nt RNA (donor) to which a 50 nt DNA primer was hybridized.

173 A blunt-ended duplex DNA primer was not utilized by telomerase.

174 say using a heteropolymeric RNA template and DNA primers, we defined enzymatic profiles of recombinan

175 Mixtures of dye-labeled, M13-forward DNA primers were separated by capillary gel electrophore

176 tic Okazaki fragments are initiated by a RNA/DNA primer, which is removed before the fragments are jo

177 After the addition of a dT residue to the DNA primer, which is specified by the 49 rA residue in t

178 Okazaki fragments are initiated by short RNA/DNA primers, which are displaced into flap intermediates

179 genomic RNA template annealed with an 18-mer DNA primer with a sequence complementary to the primer b

180 idue in the template, telomerase extends the DNA primer with three additional nucleotides and then pa

181 omplemented PNA probes at an 18:1 ratio over DNA primers with a mismatch result in suppression of amp

182 usly with an RNA primer are preserved with a DNA primer--with the same set of polymerase residues tra