ライフサイエンスコーパス: DNA shuffling

1 by generating chimeras with hASNase1 through DNA shuffling.

2 y from a cellulase library created by family DNA shuffling.

3 d on in vitro recombination methods, such as DNA shuffling.

4 dely used in vitro DNA recombination method, DNA shuffling.

5 dition to library generation methods such as DNA shuffling.

6 to analyze chimeric DNA libraries created by DNA shuffling.

7 ent protein was subjected to mutagenesis and DNA shuffling.

8 ation for the creation of hybrid enzymes and DNA shuffling.

9 crossover statistics and draws contrast with DNA shuffling.

10 eling a specific DNA recombination protocol, DNA shuffling.

11 roteins and enzymes have been improved using DNA shuffling.

12 ropic murine leukemia virus (MLV) strains by DNA shuffling.

13 qual to the activity of the hybrids found by DNA shuffling.

14 This mutagenesis approach is called DNA shuffling.

15 r multiple rounds of cycling mutagenesis and DNA shuffling, a more efficient nuclease variant (Sharke

16 After 3 cycles of DNA shuffling, a mutant was obtained with a whole cell f

17 iscipline was galvanized by the invention of DNA shuffling, a procedure that randomly recombines poin

18 Seven rounds of DNA shuffling and colony screening on chromogenic fucose

19 other into extinction (clonal interference); DNA shuffling and combinatorial cassette mutagenesis led

20 We envision that the combination of DNA shuffling and high throughput screening will be a po

21 Eight rounds of DNA shuffling and in vivo selection followed by a backcr

22 erent PRRSV strains were molecularly bred by DNA shuffling and iteration of the process, and the shuf

23 Our results suggest that combined DNA shuffling and mutagenesis of libraries of Flp varian

24 Repeated rounds of DNA shuffling and oligonucleotide-directed mutagenesis f

25 DNA shuffling and other in vitro recombination strategie

26 More specifically, DNA shuffling and other methods of genetic recombination

27 een altered by stochastic mutations based on DNA shuffling and rationally tailored by structure-based

28 utant, TG-15, was subjected to iterations of DNA shuffling and screened for enzyme variants with up-r

29 Here we use two cycles of DNA shuffling and screening in Escherichia coli to obtai

30 oped a more potent version of IL-12 by using DNA shuffling and screening to improve its expression in

31 among 32 clones isolated in three rounds of DNA shuffling and screening were mapped to the active si

32 s in specificity and activity by reiterative DNA shuffling and screening, even for an enzyme of 109 k

33 e substrate) were evolved in seven rounds of DNA shuffling and screening.

34 esus monkey, baboon, cat, cow, and rabbit by DNA shuffling and screening.

35 re recombined in two cycles of high-fidelity DNA shuffling and screening.

36 Further rounds of evolution using DNA shuffling and staggered extension process (StEP) res

37 ies, requires fewer PCR cycles than in vitro DNA shuffling and, unlike site-specific recombination me

38 tion to existing DNA recombination methods ('DNA shuffling') and should be particularly useful for re

39 f enzymes resulting from random mutagenesis, DNA shuffling, and combinatorial saturation mutagenesis

40 on of error-prone polymerase chain reaction, DNA shuffling, and multiple-site-directed mutagenesis to

41 was isolated after three rounds of mutation, DNA shuffling, and screening.

42 Our CODNS (cross-over optimization for DNA shuffling) approach employs polynomial-time dynamic

43 We describe a computational model of DNA shuffling based on the thermodynamics and kinetics o

44 eric Pgp with an altered resistance profile, DNA shuffling between the homologous but not identical d

45 DNA shuffling can be combined with screening of a modera

46 Molecular breeding via DNA shuffling can direct the evolution of viruses with d

47 These results show that DNA shuffling can improve the function of pathways by co

48 of improved variants from the two rounds of DNA shuffling confirmed important features of the recomb

49 High fidelity in vitro gene recombination ("DNA shuffling") coupled with sequence analysis of a smal

50 or assessing the generation of crossovers in DNA shuffling experiments.

51 DNA shuffling facilitated the evolution of a human immun

52 odon usage and performed recursive cycles of DNA shuffling followed by screening for the brightest E.

53 s resource-intensive and more effective than DNA shuffling for this particular evolutionary pathway.

54 An efficient beta-fucosidase was evolved by DNA shuffling from the Escherichia coli lacZ beta-galact

55 DNA shuffling generates combinatorial libraries of chime

56 While DNA shuffling has been applied quite successfully, it is

57 m mutagenesis, site-directed mutagenesis and DNA shuffling, have been widely used to generate variant

58 DNA shuffling identified the beneficial NagAc mutation G

59 Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four

60 Furthermore, DNA shuffling improved the level of expression and homog

61 These studies demonstrate the utility of DNA shuffling in breeding viral strains with improved ch

62 c resistance operon has been accomplished by DNA shuffling, involving multiple rounds of in vitro rec

63 DNA shuffling is a powerful process for directed evoluti

64 DNA shuffling is a practical process for directed molecu

65 presence of synergistic reassembly in family DNA shuffling is obtained.

66 At a molecular level, DNA shuffling mimics, yet accelerates, evolutionary proc

67 The in vitro process of DNA shuffling (molecular breeding) mimics this mechanism

68 DNA sequences for recombination, we combined DNA shuffling mutagenesis and a forward selection strate

69 DNA shuffling of a family of over 20 human interferon-al

70 Four rounds of random mutagenesis and DNA shuffling of Drosophila melanogaster 2'-deoxynucleos

71 th in vitro and in vivo, indicating that the DNA shuffling of GP4 and M genes did not significantly i

72 DNA shuffling of mutations from the various screens and

73 DNA shuffling of NDO nagAcAd also generated the NagAc va

74 this study we molecularly bred PRRSV through DNA shuffling of the GP4 and M genes, separately, from s

75 The results suggest that DNA shuffling of the GP4 or M genes from different paren

76 Here, random mutagenesis and DNA shuffling of the single-chain variable fragment of t

77 n of the virus was achieved in this study by DNA shuffling of the viral envelope genes from multiple

78 this study reveals a unique approach through DNA shuffling of viral envelope genes to attenuate a pos

79 ified as beneficial by random mutagenesis or DNA shuffling or seen in any of the naturally occurring

80 of hGSTT1-1 constructed by error-prone PCR, DNA shuffling, or saturation mutagenesis were screened f

81 etween phenotypes and variation generated by DNA shuffling paralleled natural variation observed betw

82 Three aspects of the DNA shuffling procedure are modeled: the fragment size d

83 ed chimeric sections are then subjected to a DNA shuffling process generating an enhanced crossover S

84 ults led to several unique insights into the DNA shuffling process.

85 Two subsequent rounds of DNA shuffling produced additional clones that showed fur

86 t diversity targets are investigated for the DNA shuffling protocol to showcase the utility of the eC

87 A convenient 'DNA shuffling' protocol for random recombination of homo

88 We previously used DNA shuffling (sexual recombination) and a histochemical

89 Our less biased approach to DNA shuffling should be useful for the engineering of a

90 ned sequence; (ii) minimizing bias in family DNA shuffling so that each of the parental sequence pair

91 his artificial family is then subjected to a DNA-shuffling step to augment the number of crossovers.

92 DNA shuffling technology has been significantly enhanced

93 enesis of cloned genes by error-prone PCR or DNA shuffling that eliminates the need for post-amplific

94 the protein engineer with a new approach to DNA shuffling that supports substantially more diverse p

95 We compared the ability of ITCHY and DNA shuffling to create interspecies fusion libraries be

96 We previously used DNA shuffling to direct the evolution of Escherichia col

97 We used DNA shuffling to explore and confirm the hypothesis that

98 nts efficient methods to extend the scope of DNA shuffling to handle significantly more diverse paren

99 Using DNA shuffling to introduce functional sequence variabili

100 te spectrum, we used rounds of selection and DNA shuffling to obtain GroEL/S variants that dramatical

101 oduce a method of in vitro recombination or "DNA shuffling" to generate libraries of evolved enzymes.

102 DNA shuffling was used as a combinatorial in vitro genet

103 tion mutagenesis and in vitro recombination (DNA shuffling) was used to generate mutant libraries, wh

104 combinatorial random mutagenesis technique (DNA shuffling), we have isolated an IE(ME)-specific hype

105 xplore whether there might be limits to such DNA shuffling, we have mapped the termini of mitochondri

106 antage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes n

107 nt cln2-KAEA, but additional mutagenesis and DNA shuffling yielded multiply mutant CDC28-BYC alleles