ライフサイエンスコーパス: DNA strand exchange

1 ing the ATP hydrolysis to work (in this case DNA strand exchange).

2 e complementation is not sufficient to allow DNA strand exchange.

3 in eukaryotes performing homology search and DNA strand exchange.

4 r homologous double-stranded DNA (dsDNA) and DNA strand exchange.

5 stigated the hRad54-dependent stimulation of DNA strand exchange.

6 llow the movement of protein subunits during DNA strand exchange.

7 tein C terminus that activates RecA-mediated DNA strand exchange.

8 forming presynaptic filaments that initiate DNA strand exchange.

9 including ssDNA-dependent ATP hydrolysis and DNA strand exchange.

10 binding (SSB) protein, thereby accelerating DNA strand exchange.

11 the hydrolysis of dATP is poorly coupled to DNA strand exchange.

12 52 protein stimulates Rad51 protein-promoted DNA strand exchange.

13 stuck on the heteroduplex DNA product after DNA strand exchange.

14 tions, Rad52 protein is needed for extensive DNA strand exchange.

15 lished joint molecules in Rad51/Rpa-mediated DNA strand exchange.

16 nds on ATP to promote homologous pairing and DNA strand exchange.

17 ding specificity in a manner that stimulates DNA strand exchange.

18 hanistic coupling between NTP hydrolysis and DNA strand exchange.

19 HOP2-MND1 as a 'molecular trigger' of RAD51 DNA strand exchange.

20 ssDNA, and stimulates Rad51 protein-mediated DNA strand exchange.

21 romotes ATP-dependent homologous pairing and DNA strand exchange.

22 lf to explain how ATP hydrolysis facilitates DNA strand exchange.

23 and the gene 4 helicase, mediate homologous DNA strand exchange.

24 The protein nevertheless promotes DNA strand exchange.

25 he secondary binding site has a dual role in DNA strand exchange.

26 A S119A repressor blocks a site required for DNA strand exchange.

27 sDNA to the secondary site strongly inhibits DNA strand exchange.

28 DNA, and contribute to the directionality of DNA strand exchange.

29 y, the ssDNA strand that is displaced during DNA strand exchange.

30 synapsis occurs during RecA protein-mediated DNA strand exchange.

31 Dmc1-Tid1 tilt the bias toward interhomolog DNA strand exchange.

32 the search for homologous DNA sequences and DNA strand exchange.

33 ion results in stimulation of RAD51-promoted DNA strand exchange.

34 centration dependence parallels that seen in DNA strand exchange.

35 ricus Rad51 homologue, SsoRadA, to stimulate DNA strand exchange.

36 ded DNA (ssDNA), but not dsDNA, to stimulate DNA strand exchange.

37 the current models linking ATP hydrolysis to DNA strand exchange.

38 olysis is completely uncoupled from extended DNA strand exchange.

39 ional separation of DNA pairing and extended DNA strand exchange.

40 ys a major role in, and may be required for, DNA strand exchange.

41 s DNAs are paired and available for extended DNA strand exchange.

42 a tight coupling between ATP hydrolysis and DNA strand exchange.

43 that binds RAD51, the enzyme responsible for DNA strand exchange.

44 on of UvsX-ssDNA filaments that is active in DNA strand exchange.

45 sDNA overhang that serves as a substrate for DNA strand exchange.

46 ed RecA from DNA and inhibited RecA-mediated DNA strand exchange.

47 element in promoting homologous pairing and DNA strand exchange.

48 ate Rad51 from dsDNA, the product complex of DNA strand exchange.

49 context of a kinetic model for RecA-mediated DNA strand exchange.

50 oviding new insights into how RecA catalyses DNA strand-exchange.

51 with each recombinase mediating one pair of DNA strand exchanges.

52 naptic complex and orchestrates the order of DNA strand exchanges.

53 helical nucleoprotein filament that promotes DNA strand exchange, a basic step of homologous recombin

54 RecA protein catalyzes DNA strand exchange, a basic step of homologous recombin

55 Rad51 mediates DNA strand exchange, a key reaction in DNA recombination

56 Rad51 mediates DNA strand exchange, a key reaction in DNA recombination

57 The Rad51 nucleoprotein filament mediates DNA strand exchange, a key step of homologous recombinat

58 RecA protein promotes DNA strand exchange, a reaction that contributes to fork

59 In addition to increased DNA-strand exchange, a cytogenetic feature of cells lack

60 In vitro, HOP2-MND1 stimulates the DNA strand exchange activities of RAD51 and DMC1.

61 A (RPA) on presynaptic complex formation and DNA strand exchange activities of Rad51 protein were exa

62 ded DNA (ssDNA)-dependent ATP hydrolysis and DNA strand exchange activities of RecA protein.

63 ons of Srs2, providing a means for tailoring DNA strand exchange activities to enhance the fidelity o

64 To accomplish its DNA strand exchange activities, the Escherichia coli pro

65 rotein is a novel inhibitor of RecA-mediated DNA strand exchange activities.

66 In vitro, RAD52 has ssDNA annealing and DNA strand exchange activities.

67 e activities, as well as in vitro ATPase and DNA strand exchange activities.

68 acidic conditions restore both the in vitro DNA strand exchange activity and the in vivo function of

69 ve for genetic recombination in vivo and for DNA strand exchange activity in vitro under conventional

70 In contrast, inhibition of DNA strand exchange activity is SSB protein-independent,

71 The DNA strand exchange activity of hDmc1 is probably indisp

72 Here, we show that the DNA strand exchange activity of hDmc1 protein is also st

73 results show that Ca(2+) greatly stimulates DNA strand exchange activity of hRad51 protein.

74 for hRad54 protein to effectively stimulate DNA strand exchange activity of hRad51 protein.

75 role of the ATPase activity in regulation of DNA strand exchange activity of hRad51 protein.

76 emonstrated that Ca2+ greatly stimulates the DNA strand exchange activity of human (h) Rad51 protein.

77 und 1 (B02), which specifically inhibits the DNA strand exchange activity of human RAD51.

78 Surprisingly, we found that BLM stimulates DNA strand exchange activity of RAD51.

79 The DNA strand exchange activity of RecA P67W protein is als

80 The UvsX-H195Q mutant retains weak DNA strand exchange activity that is inhibited by wild-t

81 Rad51C displays an ATP-independent apparent DNA strand exchange activity, whereas Rad51B shows no su

82 ant protein has a corresponding reduction in DNA strand exchange activity, which probably results in

83 her Lys6 or Arg28 show partial inhibition of DNA strand exchange activity, yet the mechanistic reason

84 ngly with reduced ssDNA-binding affinity and DNA strand-exchange activity in both H195Q and H195A mut

85 filament has long been known to occur during DNA strand exchange, although its importance to this pro

86 NA/hRAD51 and including salts that stimulate DNA strand exchange (ammonium sulfate and spermidine) we

87 nucleoprotein filament is more competent in DNA strand exchange and acts over a broader range of sol

88 Rad51 protein promotes DNA strand exchange and acts similarly to RecA protein.

89 e Rad51-G103E mutant protein is deficient in DNA strand exchange and ATPase activity due to a primary

90 nteractions between hRad54 and hRad51 during DNA strand exchange and branch migration, which are two

91 RAD54 promotes RAD51-mediated DNA strand exchange and has been described to both stabi

92 In vitro, RAD54 stimulates RAD51-mediated DNA strand exchange and promotes branch migration of Hol

93 Rad51 nucleoprotein filaments catalyze DNA strand exchange and Rad54 augments this activity of

94 f RecX strongly inhibited both RecA-mediated DNA strand exchange and RecA ATPase activity.

95 verexpression of Rad51, a protein central to DNA strand exchange and recombination, did not further i

96 Rad51 and annealing DNA, are coordinated in DNA strand exchange and second ssDNA capture.

97 d in vivo, stimulating RecA protein-mediated DNA strand-exchange and rescuing the ssb-1 lethal mutati

98 om the work are 13.3 +/- 1.1 kcal mole-1 for DNA strand exchange, and 14.4 +/- 1.4 kcal mole-1 for AT

99 eoprotein filament makes dsDNA receptive for DNA strand exchange, and it defines an early step of the

100 of the RecA protein, including DNA binding, DNA strand exchange, and LexA protein cleavage.

101 1 protein (hDmc1) for the ability to promote DNA strand exchange, and show that hDmc1 mediates strand

102 TP, in concert with SsbA and DprA, catalyzes DNA strand exchange, and SsbB is an accessory factor in

103 We refer to this novel DNA strand exchange as being in trans.

104 All RecA-like recombinase enzymes catalyze DNA strand exchange as elongated filaments on DNA.

105 s affect the pK(a) of key groups involved in DNA strand exchange as well as the direct binding of Rec

106 51 protein requires ATP for the catalysis of DNA strand exchange, as do all Rad51 and RecA-like recom

107 ies preference in the Rad51 dissociation and DNA strand exchange assays underlines the importance of

108 RdgC inhibits RecA protein-promoted DNA strand exchange, ATPase activity, and RecA-dependent

109 acid residues result in strong inhibition of DNA strand exchange below pH 7, where the wild-type prot

110 We have examined DNA strand exchange between a 70 nucleotide ssDNA fragme

111 protein of HR, possesses a unique activity: DNA strand exchange between homologous DNA sequences.

112 Although providing an efficient rate of DNA strand exchange between polymorphic alleles, Dmc1 mu

113 ength BRCA2 protein stimulates DMC1-mediated DNA strand exchange between RPA-ssDNA complexes and dupl

114 In vitro, the protein mediates DNA strand exchange between single-stranded (ssDNA) and

115 All three variants are proficient in DNA strand exchange, but G151D is slightly more sensitiv

116 red for DNA repair, including RAD51 mediated DNA strand exchange, but is dispensable for DNA replicat

117 ATP and ssDNA active in homology search and DNA strand exchange, but the precise role of its ATPase

118 rming homologues identified to date, promote DNA strand exchange by a common, ordered pathway.

119 The resolvase Sin regulates DNA strand exchange by assembling an elaborate interwoun

120 studies established that Brh2 can stimulate DNA strand exchange by enabling Rad51 nucleoprotein fila

121 ing of the ssDNA strand that is displaced by DNA strand exchange by Rad51 and RPA, to a second ssDNA

122 ion of Rad54 protein overcomes inhibition of DNA strand exchange by Rad51 protein bound to substrate

123 Only the first group enhances DNA strand exchange by RAD51.

124 DNA networks, leading to rapid and efficient DNA strand exchange by Rad51.

125 excess of ssDNA and prevents the reversal of DNA strand exchange by removing the displaced strand fro

126 additional role in the postsynaptic phase of DNA strand exchange by stimulating heteroduplex DNA exte

127 We find that Rad52 protein stimulates DNA strand exchange by targeting Rad51 protein to a comp

128 breaks by homologous recombination, promotes DNA strand-exchange by an unprecedented inverse pathway,

129 DNA strand exchange catalyzed by Rad51 protein is also g

130 RdgC protein can inhibit DNA strand exchange catalyzed by RecA nucleoprotein fila

131 ede activities of RecA that are important to DNA strand exchange, consistent with its role in targeti

132 sbA, but not SsbB, DprA was able to activate DNA strand exchange dependent on RecA . ATP.

133 The products of extended DNA strand exchange do not form.

134 yotic recombinase responsible for initiating DNA strand exchange during homologous recombination.

135 herichia coli RecA protein, an ATP-dependent DNA strand exchange factor.

136 has a more stringent requirement to initiate DNA strand exchange from the P state.

137 requirement for RecA filament disassembly in DNA strand exchange has a variety of ramifications for t

138 teins align homologous sequences and promote DNA strand exchange has long been known, as are the crys

139 ic recombinase Dmc1 plays a critical role in DNA strand exchange in budding yeast.

140 The Escherichia coli RecA protein promotes DNA strand exchange in homologous recombination and reco

141 These proteins normally promote DNA-DNA strand exchange in homologous recombination.

142 2+ effects, the limitations of RecA-mediated DNA strand exchange in the absence of ATP hydrolysis, an

143 It enables RAD51 DNA strand exchange in the absence of divalent metal ion

144 DprA facilitates RecA-mediated DNA strand exchange in the presence of both SSB proteins

145 substrates for the RecA protein, permitting DNA strand exchange in vitro at a rate and efficiency co

146 us recombination in vivo, is able to perform DNA strand exchange in vitro with ATP, but is unable to

147 pairs homologous DNA molecules and promotes DNA strand exchange in vitro.

148 f the dimer to cleave DNA and most abolished DNA strand exchange in vitro.

149 sults suggest that UTP may be a cofactor for DNA strand exchange in vivo.

150 Optimal conditions for RecA protein-mediated DNA strand exchange include 6-8 mm Mg(2+) in excess of t

151 When ATP is hydrolyzed, DNA strand exchange is accompanied by a RecA exchange re

152 laments improves at pH 8.5, whereas complete DNA strand exchange is also restored.

153 DNA strand exchange is also slowed commensurate with the

154 d significant ATP is hydrolyzed, even though DNA strand exchange is entirely blocked by the mutant pr

155 Rad51-catalyzed DNA strand exchange is greatly enhanced by the single-st

156 DNA strand exchange is greatly facilitated by the E. col

157 nd, as a consequence, Rad51 protein-mediated DNA strand exchange is inhibited when the ssDNA is in a

158 the four-stranded intermediate arising from DNA strand exchange is migrated and resolved and how anc

159 How DNA mismatches affect Dmc1-mediated DNA strand exchange is not understood.

160 tween filament disassembly and completion of DNA strand exchange is observed.

161 he secondary site for ssDNA is essential for DNA strand exchange, it renders DNA strand exchange sens

162 aptically to inhibit RecA during an on-going DNA strand exchange, likely through the disassembly of R

163 ate common and idiosyncratic features in the DNA strand exchange mechanisms of three RecA-family reco

164 filaments possess an intrinsic capacity for DNA strand exchange, mediated by binding energy rather t

165 s DNA molecules and promote highly efficient DNA strand exchange of the paired molecules over at leas

166 pHs 7.5 and 8.1, dATP supports an efficient DNA strand exchange only at pH 8.1.

167 DNA strand exchange plays a central role in genetic reco

168 accharomyces cerevisiae (ScRad51) propagated DNA strand exchange preferentially in the 5' to 3' direc

169 y nevertheless play an important role in the DNA strand exchange process.

170 context of a model in which a fast phase of DNA strand exchange produces a discontinuous three-stran

171 g identifies Ca2+ as a universal cofactor of DNA strand exchange promoted by mammalian homologous rec

172 he main reason for the low efficiency of the DNA strand exchange promoted by Rad51 protein in vitro i

173 xplain some of the unique characteristics of DNA strand exchange promoted by Rad51 protein, when comp

174 we investigated the role of Mer3 helicase in DNA strand exchange promoted by Rad51 protein.

175 est a common molecular process underlies the DNA strand exchange promoted by RecA and Rad51.

176 cD and its cognate RecA led to inhibition of DNA strand exchange promoted by RecA.

177 The observed course of DNA strand exchange promoted by the RecA protein from th

178 ation, and stimulates homologous pairing and DNA strand exchange promoted in vitro by human recombina

179 RAD51 DNA strand exchange protein catalyzes the central step i

180 hese features depend on the meiosis-specific DNA strand exchange protein Dmc1 (disrupted meiotic cDNA

181 In eukaryotes, the Rad51 DNA strand exchange protein is assisted in D loop format

182 The DNA strand exchange protein RAD51 facilitates the centra

183 The meiosis-specific DNA strand exchange protein, DMC1, promotes the formatio

184 Here we show that the Homo sapiens DNA strand exchange protein, HsRad51, shows a preference

185 Here we report that the S. cerevisiae DNA strand exchange protein, Rad51, prevents Rad52-media

186 overhang, which becomes a substrate for the DNA strand exchange protein, Rad51.

187 he RecBCD enzyme and leads to loading of the DNA strand exchange protein, RecA, onto the chi-containi

188 cBCD enzyme to coordinate the loading of the DNA strand exchange protein, RecA, onto the single-stran

189 in, RadA, possesses the characteristics of a DNA strand exchange protein: The RadA protein is a DNA-d

190 homolog of the RecA protein, the prototypic DNA strand-exchange protein of Escherichia coli.

191 itiation of recombination and for loading of DNA strand-exchange protein RAD-51, despite the fact tha

192 sal role of Ca2+ in stimulation of mammalian DNA strand exchange proteins and reveal diversity in the

193 The RecA protein and other DNA strand exchange proteins are characterized by their

194 Both of these properties are common to all DNA strand exchange proteins examined thus far.

195 rocessed ends are substrates for assembly of DNA strand exchange proteins that mediate DNA strand inv

196 tural domains that interact with and recruit DNA strand exchange proteins to DNA.

197 nuc also interacts with and loads noncognate DNA strand exchange proteins.

198 ther extend the universal characteristics of DNA strand exchange proteins.

199 is the paradigm for eukaryotic ATP-dependent DNA strand exchange proteins.

200 the defining member of a ubiquitous class of DNA strand-exchange proteins that are essential for homo

201 RPA(rfa1-t11) stimulates DNA strand exchange, provided that the Rad51 protein.ssD

202 After DNA strand exchange Rad51 protein is stuck on the double

203 mutant protein is capable of catalyzing the DNA strand exchange reaction and is insensitive to inhib

204 h the presynaptic and synaptic phases of the DNA strand exchange reaction as follows: during presynap

205 as nucleoside triphosphate cofactor for the DNA strand exchange reaction in vitro.

206 rate that PcrA can inhibit the RecA-mediated DNA strand exchange reaction in vitro.

207 Deinococcus radiodurans (Dr) both promote a DNA strand exchange reaction involving two duplex DNAs.

208 erse" reaction is a unique, highly efficient DNA strand exchange reaction that is not due to redistri

209 RecA protein promotes a limited DNA strand exchange reaction, without ATP hydrolysis, th

210 serves as a mediator in the Rad51-catalyzed DNA strand exchange reaction.

211 on of mixed filaments and a poisoning of the DNA strand exchange reaction.

212 The RAD51 encoded product mediates the DNA strand exchange reaction.

213 The DNA strand-exchange reaction catalyzed by the Escherichi

214 RecA protein catalyses an ATP-dependent DNA strand-exchange reaction that is the central step in

215 e Escherichia coli RecA protein performs the DNA strand-exchange reaction utilized in both genetic re

216 Rad51 protein catalyzes the DNA strand-exchange reaction with a dependence on ATP an

217 protein filament can participate in multiple DNA strand exchange reactions concurrently (involving du

218 The yield of DNA strand exchange reactions driven by both Escherichia

219 at improve DNA pairing can inhibit extensive DNA strand exchange reactions in the absence of ATP hydr

220 ights into the role of ATP hydrolysis in the DNA strand exchange reactions promoted by the bacterial

221 , optimal conditions for the DNA pairing and DNA strand exchange reactions promoted by the RecA and R

222 e of ATP hydrolysis in RecA protein-mediated DNA strand exchange reactions remains controversial.

223 In DNA strand exchange reactions using oligonucleotides, we

224 combined biochemical reconstitutions of the DNA strand exchange reactions with total internal reflec

225 ort shape changing films that are powered by DNA strand exchange reactions with two different domains

226 l upward shift in the pH-reaction profile of DNA strand exchange reactions.

227 f RecA in DNA metabolism is the promotion of DNA strand exchange reactions.

228 protein hydrolyzes ATP and dATP and promotes DNA strand exchange reactions.

229 competent to catalyze homologous pairing and DNA strand exchange reactions.

230 A and the function of Rad51 in ATP-dependent DNA strand exchange reactions.

231 ters the properties of RecA protein-mediated DNA strand exchange reactions.

232 and two molecules each of XerC and XerD, the DNA strand-exchange reactions are separated in time and

233 Its presence in DNA strand-exchange reactions in vitro results in a sign

234 osterically activated to catalyze ATPase and DNA strand-exchange reactions.

235 DNA strand exchange requires ATP and is strongly depende

236 Protein-promoted DNA strand exchange requires formation of an active pres

237 erminal residues eliminates the reduction in DNA strand exchange seen with the wild-type protein at p

238 ssential for DNA strand exchange, it renders DNA strand exchange sensitive to an excess of ssDNA whic

239 to explain how ATP hydrolysis is coupled to DNA strand exchange so as to bring about these effects.

240 that RPA has a critical postsynaptic role in DNA strand exchange, stabilizing the DNA pairing initiat

241 displaced from homologous duplex DNA during DNA strand exchange, stabilizing the initial heteroduple

242 chia coli RecA protein catalyzes the central DNA strand-exchange step of homologous recombination, wh

243 RecA protein promotes homologous pairing and DNA strand exchange, steps important to homologous recom

244 acement to generate PiDSD, an intermolecular DNA strand-exchange strategy to measure a set of key kin

245 bility of RecA to utilize UTP as cofactor in DNA strand exchange suggest a separation of the function

246 DNA strand exchange, the central step of homologous reco

247 During DNA strand exchange, the primary site binds to single-st

248 omote a search for homology and that perform DNA strand exchange, the two essential steps of genetic

249 though Cre and Int use the same mechanism of DNA strand exchange, their respective reaction pathways

250 the nucleoprotein filament that is active in DNA strand exchange, these findings raise the possibilit

251 At both pHs, ATP supports efficient DNA strand exchange through heterologous insertions but

252 Models for the coupling of DNA strand exchange to ATP hydrolysis are examined.

253 ivity that can couple the branch movement in DNA strand exchange to ATP hydrolysis.

254 ant for the search of homologous DNA and for DNA strand exchange, two critical steps of homologous re

255 ATP hydrolysis renders DNA strand exchange unidirectional, greatly increases th

256 The optimal conditions for Rad51-mediated DNA strand exchange used here minimize the secondary str

257 fects both presynaptic complex formation and DNA strand exchange via changes in DNA structure, employ

258 Initiation of DNA strand exchange was affected in a DNA structure-depe

259 gly, the ability of RAD54 to stimulate RAD51 DNA strand exchange was not significantly affected by SN

260 stranded oligonucleotides were activated for DNA strand exchange when attached as tails protruding fr

261 Unlike Rad51, Brh2 was able to promote DNA strand exchange when preincubated with double-strand

262 d54 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias tow

263 o a gapped duplex DNA molecule and promote a DNA strand exchange with a second homologous linear dupl

264 As a consequence, RecA-RFP is proficient for DNA strand exchange with dATP or at lower pH.

265 double-stranded (ds) DNA, an intermediate in DNA strand exchange with unclear functional significance

266 Optimization of an extensive DNA strand exchange without ATP hydrolysis requires cond