ライフサイエンスコーパス: DNA template

1 -stranded DNA breaks or provision of a donor DNA template.

2 allowing for a semi-quantitative analysis of DNA template.

3 ge of the transcript without movement of the DNA template.

4 e, specific, and sensitive down to 0.32ng of DNA template.

5 10 nm in width, and take on the shape of the DNA template.

6 e produced is limited only by the underlying DNA template.

7 then closes to establish a tight grip on the DNA template.

8 hat increase the rigidity of the neutralized DNA template.

9 de nanostructure inherits its shape from the DNA template.

10 ontinuity of DNA replication on an undamaged DNA template.

11 g complex and in sequence recognition in the DNA template.

12 ers a remarkable rearrangement of enzyme and DNA template.

13 diting genomes when codelivered with a donor DNA template.

14 with a double- and/or single-stranded donor DNA template.

15 araensis p41-p46 complex in the absence of a DNA template.

16 onsible for messenger RNA synthesis from the DNA template.

17 of specific sequences in the single-stranded DNA template.

18 thesized by varying the base sequence of the DNA template.

19 s priming the ParB for polymerization on the DNA template.

20 ctivity of UDG tethers the holoenzyme to the DNA template.

21 4 copies per cell, approximately one RNA per DNA template.

22 the nascent RNA product is released from the DNA template.

23 X9 activated transcription of a naked Col2a1 DNA template.

24 ld-type maternal gene instead of a synthetic DNA template.

25 which has minor groove interactions with the DNA template.

26 AM was present in an amount equimolar to the DNA template.

27 lty bypassing a single rNMP present within a DNA template.

28 s are tethered to RNAP II on the immobilized DNA template.

29 determined by the sequence of the underlying DNA template.

30 had higher activity from a more supercoiled DNA template.

31 tin packing during processing of the damaged DNA template.

32 e first one to face the opposite side of the DNA template.

33 result in two or more RNA transcripts from a DNA template.

34 ers of information can be stored in a single DNA template.

35 milar to the one observed with an unmodified DNA template.

36 echanochemical coupling in a single-molecule DNA template.

37 a inhibition in the presence of a platinated DNA template.

38 nfluence TFs that are already present on the DNA template.

39 s the synthesis of the messenger RNA using a DNA template.

40 t simplifies the preparation of roadblocking DNA templates.

41 e sequences in ensembles of nearly identical DNA templates.

42 I-dependent transcription of single-stranded DNA templates.

43 stalling during bypass of ribonucleotides in DNA templates.

44 se in mutation frequency when copying gapped DNA templates.

45 ll four nucleobases on homopolymeric RNA and DNA templates.

46 TP or dGTP into complementary, homopolymeric DNA templates.

47 This switch occurs only on RNA and not on DNA templates.

48 he effect of activators, compared with naked DNA templates.

49 ter reconstituted on naked and chromatinized DNA templates.

50 alt concentrations or negatively supercoiled DNA templates.

51 ctions of holo-TFIID molecules at individual DNA templates.

52 racts directly with PAF1c and recruits it to DNA templates.

53 (TLS) and by their low fidelity on undamaged DNA templates.

54 nd LacI can induce a Delta Lk to the plasmid DNA templates.

55 RNA and DNA polymerases, using both RNA and DNA templates.

56 in the catalytically competent state on pure DNA templates.

57 h leading and lagging strand single-stranded DNA templates.

58 and other NRTIs, when complexed with RNA or DNA templates.

59 of transcriptional factors to the accessible DNA templates.

60 ing transcription only from extrachromosomal DNA templates.

61 operly suppress DNA synthesis on UVB-damaged DNA templates.

62 ulating the types and ratios of the circular DNA templates.

63 ass synthesis on oxidative damage-containing DNA templates.

64 directly observe TALE search dynamics along DNA templates.

65 omponents in spite of an excess of the other DNA templates.

66 , with respect to the deoxyribonucleic acid (DNA) template.

67 lex binding inhibits the formation of duplex DNA templating.

68 ent ability to faithfully and rapidly copy a DNA template according to precise Watson-Crick base pair

69 T7DNAP advanced on a DNA template against an 80-mV load applied across the na

70 es allowed the quantitative determination of DNA template amounts.

71 a) in the ternary complex with an RNA-primed DNA template and aphidicolin.

72 ation proceeds sequence specifically along a DNA template and can generate polymers of at least 50 bu

73 RT to the same extent on either an RNA or a DNA template and did not alter the RNase H cleavage patt

74 a in unliganded form, bound to an RNA primer/DNA template and extending an RNA primer with deoxynucle

75 second strand synthesis of a single-stranded DNA template and generate millions of pair-wise combinat

76 iated by signals directly encoded within the DNA template and nascent RNA, whereas Rho-dependent term

77 ion happens in the context of defects in the DNA template and other forms of replication stress that

78 Interactions of both domains with the DNA template and ribonucleotides are required for primer

79 nown transcriptional properties of any given DNA template and set of experimental conditions, includi

80 e rolling circle amplification of a circular DNA template and simultaneous overlap extension by therm

81 single stranded DNA or RNA using a circular DNA template and special DNA or RNA polymerases.

82 elping to stabilize the position of both the DNA template and the incoming nucleotide.

83 binding and also defines the elements of the DNA template and the RNA primer that interact with p58C.

84 ocess of transcription alters the underlying DNA template and thereby modifies the genetic landscape.

85 I integrates inputs from both strands of the DNA template and three dedicated protein subunits to tri

86 factors interacts directly with the promoter DNA template and with RNA polymerase (RNAP) holoenzyme.

87 hinery gains access to damaged chromatinized DNA templates and how the chromatin structure is modifie

88 PCR assembly of DNA templates and in vitro transcription allow synthesis

89 We used various DNA templates and inhibitors to compare the performance

90 ed towards the recovery of CpG-rich and long DNA templates and is limited by the fast post-mortem cyt

91 on, and amplification on a library of 10(14) DNA templates and observed approximately 380-fold enrich

92 ts between the initial concentrations of HBV DNA templates and the system response (DeltaRU) at varyi

93 two serines or two phosphoserines, from one DNA template, and demonstrate programmable kinase activi

94 f Arg(444) and Arg(447) in stacking with the DNA template, and of Arg(448) and Gln(440) in helping to

95 hat accumulate during replication of damaged DNA templates, and also clarify the roles played by Top3

96 RNA templates are generally superior to DNA templates, and oligo-ribo-T templates are superior t

97 ombinant Daxx assembles H3.3/H4 tetramers on DNA templates, and the ATRX-Daxx complex catalyzes the d

98 ere composed of a minicircle single-stranded DNA template annealed to primers that contained 5' DNA f

99 by bacteriophage T7 RNAP on small, circular DNA templates approximately 100 bp in size.

100 Single rNMPs embedded in a DNA template are known to induce cellular DNA polymerase

101 In this strategy, DNA templates are circularized, copied multiple times in

102 nts from a surface-bound RNA primer, and the DNA templates are enzymatically destroyed, leaving behin

103 How pol II recognizes DNA template backbone (phosphodiester linkage and sugar)

104 ply of nucleotides, and the condition of the DNA template (both in terms of sequence context and the

105 ome duplication in the absence of a pristine DNA template, but identification of the enzymes involved

106 se epimer 2, was readily incorporated into a DNA template by HIV reverse transcriptase to act as a DN

107 DNA replication requires priming of DNA templates by enzymes known as primases.

108 Initiation of RNA synthesis from DNA templates by RNA polymerase (RNAP) is a multi-step p

109 ing, and removal of collided RNAPII from the DNA template can be achieved via ubiquitylation-directed

110 e show that serial replication of individual DNA templates can be achieved by DNA polymerases held at

111 both homopolymeric and mixed-sequence 3'-NP-DNA templates can be copied into complementary 3'-NP-DNA

112 of individual RNAP molecules transcribing a DNA template carrying tandem repeats encoding the his pa

113 Defects in the DNA template challenge genetic and epigenetic inheritanc

114 tion and testing of recombinant proteins and DNA templates, clustering DNA templates on a flowcell, H

115 discriminate against the modification of the DNA template compared to the incoming nucleotide.

116 DNA templates contained a single adduct opposite either

117 RNA polymerase II (Pol II) in complex with a DNA template containing oxidized 5mCs, revealing specifi

118 We propose a model in which DNA templates containing homopolymeric nucleotide runs,

119 e visualized the binding of BCDX2 and CX3 to DNA templates containing replication forks and Holliday

120 ase that interact with the dNTP substrate or DNA template could alter virus replication.

121 le-base nucleotide incorporation into primed DNA templates covalently attached to the surface of a gl

122 In addition, we report on DNA-templated cross-linking of PNA probes as a way to pr

123 isplacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microb

124 ions that arise from polymerase errors or by DNA template damage, are unknown.

125 Polymerization of a long DNA template demonstrated the ability to use the system

126 A polymerase II phosphorylated at Ser-5 in a DNA template-dependent manner and can alter the global g

127 ices that achieve complex functionalities by DNA-templated design steered by structural feedback.

128 matin and, consequently, are central to many DNA template-directed processes including replication, r

129 biquitous protein that is essential for this DNA template-directed repair is RecA.

130 In conjunction with RNA- and DNA-templated DNA synthesis, a hydrolytic activity of th

131 diated through chemical modifications of the DNA template, DNA-associated proteins, and RNA-mediated

132 en polymerase II (Pol II) and a heteroduplex DNA template do not depend on general transcription fact

133 d synthesizes RNA without movement along the DNA template, drawing downstream DNA into itself in a pr

134 e found the BHLF1 RNA stably annealed to its DNA template during the early steps of lytic reactivatio

135 RNA polymerase (RNAP) is dislodged from the DNA template either at specific DNA sequences, called th

136 gp5/trx complex binds tightly to a primer-DNA template enabling the polymerization of hundreds of

137 hat this conformational switch might control DNA template engagement and release, modulating replisom

138 ional chromatin unit that affects nearly all DNA-templated events in eukaryotic genomes.

139 otein-DNA interactions and in turn influence DNA-templated events.

140 d by the binding of the nascent RNA with its DNA template exposes the nontemplate DNA strand to mutag

141 polymerase processing multiple homopolymeric DNA templates extended over 600 s and through >10,000 bo

142 nuclear factors ensure efficient binding to DNA templates, facilitating RNA polymerase II recruitmen

143 in the presence of a guide RNA and repairing DNA template flanked by homology DNA fragments to the ta

144 detection sensitivity of 3.25 pg or 14 nM of DNA template for ctxA gene detection.

145 protein responsible for preparing the viral DNA template for initiation of DNA replication.

146 precisely localize multiple components on a DNA template for potential applications in nanophotonic,

147 d and completely in vitro method to generate DNA templates for cell-free systems, bypassing the need

148 Discriminative base motifs within DNA templates for fluorescent silver clusters are identi

149 Preparation of DNA templates for replication requires opening of the du

150 uccessfully use click chemistry to construct DNA templates for sgRNA expression and show, rather than

151 mplexes remain attached to the same pairs of DNA templates found in vivo.

152 We used DNA templates from the pathogen Mycobacterium tuberculos

153 or cell-free systems, bypassing the need for DNA template generation and amplification from living ce

154 the synthesis and characterization of a new DNA-templated gold nanocluster (AuNC) of approximately 1

155 pel backward translocation of RNAP along the DNA template in an elongation complex.

156 rimase-helicase hexamer that assemble on the DNA template in an RNA-dependent manner.

157 The DNA template in each aliquot is amplified by multiple di

158 ription elongation complex downstream on the DNA template in the absence of transcription elongation.

159 strand of the open reading frame 50 (ORF50) DNA template in the genome of Kaposi's sarcoma-associate

160 phosphorolysis of a DNA primer annealed to a DNA template in the presence of pyrophosphate (PP(i)).

161 ed on an SSB-coated single-stranded circular DNA template in the presence of the beta/gamma complex a

162 DR)-mediated gene targeting using long donor DNA templates in hPSCs with these systems.

163 assay to follow transcription on individual DNA templates in real time.

164 tides and that RNA templates are superior to DNA templates in template-directed nonenzymatic primer-e

165 ealed for the first time that hRap1 binds to DNA templates in the absence of hTRF2 with a preference

166 We immobilized primed DNA templates in the microreactors, then sequentially in

167 with this phenotype, PPL2 replicates damaged DNA templates in vitro, including templates containing t

168 ucleotides, a pipeline of primer assembly of DNA templates, in vitro transcription by T7 RNA polymera

169 ect detection of modified nucleotides in the DNA template, including N6-methyladenine, 5-methylcytosi

170 complexity more evident than in challenging DNA templates, including highly repetitive or transcribe

171 mechanism that acts specifically on episomal DNA templates independently of the nature of the cis-reg

172 ent biological complex or process using only DNA templates instead of purified proteins.

173 rate and fidelity in the copying of a 3'-NP-DNA template into a complementary strand of 3'-NP-DNA.

174 embly technique that folds a single-stranded DNA template into a target structure by annealing it wit

175 The transcription of a gene from its DNA template into an mRNA molecule is the first, and mos

176 that enables the enzyme-free translation of DNA templates into sequence-defined synthetic polymers t

177 clude that the role of ICP0 is to render the DNA templates introduced by transfection or infection ac

178 two ligands through their influence on their DNA template is determined by a subtle interplay of DNA

179 entral step in gene expression, in which the DNA template is processively read by RNA polymerase II (

180 nucleotide specified by a single base in the DNA template is repetitively added to the nascent transc

181 In DNA origami, single-stranded DNA template is shaped into desired nanostructure by DNA

182 ions that are present in a small fraction of DNA templates is essential for progress in several areas

183 y, inferring the long-range structure of the DNA templates is limited by short read lengths.

184 n from one molecule to another in analogy to DNA templating its sequence.

185 Swarm priming is presented for three DNA templates: Lambda phage, Synechocystis sp. PCC 6803

186 p contrast, the presence of 2'-5' linkage in DNA template leads to dramatic decreases in both transcr

187 raction of CCMV capsid protein with this RNA-DNA template leads to selective packaging of the RNA por

188 ow that PolB1 repeatedly disengages from the DNA template, leaving PCNA123 behind.

189 hat comprise nascent RNA hybridized with the DNA template, leaving the nontemplate DNA single-strande

190 cally active IDE inhibitor identified from a DNA-templated macrocycle library.

191 structures or topological stress within the DNA template may lead to stalling of the replication for

192 ding to GA-rich regions of a single-stranded DNA template may promote non-specific amplification in E

193 er these data suggest that rNMPs embedded in DNA templates may influence reverse transcription kineti

194 block polymerase movement, since genes with DNA template melting showed no evidence of slowed elonga

195 t of rRNA genes, as manifested by regions of DNA template melting.

196 Dpo4 binding conformations and activity with DNA templates modified with the carcinogenic DNA adducts

197 Barcoding of DNA template molecules early in next-generation sequenci

198 same principles but is applied to individual DNA template molecules.

199 s at multiple positions of a double stranded DNA template, monomer, dimer, and trimer STV-DNA assembl

200 This DNA-templated multichromophore system serves as a modula

201 Previously, we described the DNA-templated multistep synthesis of a 13,824-membered s

202 f human Pol II transcription from individual DNA templates, observed attenuation of transcription by

203 Compared with a DNA template of the same sequence, the rate of chemistry

204 DNA templates of clinically relevant single-nucleotide m

205 in the AFM studies: the relative success of DNA templating of polymers compared to metals; the slow

206 and transcription translocate along the same DNA template, often in opposing directions and at differ

207 e the application of T4 DNA ligase-catalyzed DNA templated oligonucleotide polymerization toward the

208 in-depth analysis of T4 DNA ligase-catalyzed DNA templated oligonucleotide polymerization toward the

209 ng platforms are based on the tailoring of a DNA template on which the recognition of the target DNA

210 inant proteins and DNA templates, clustering DNA templates on a flowcell, HiTS and protein binding wi

211 e mechanistic impacts of an rNMP embedded in DNA templates on HIV-1 RT-mediated DNA synthesis.

212 ds, which employs an electrical actuation of DNA templates on microelectrodes.

213 DNA-templated organic synthesis enables the translation

214 to be responsible for translocation in many DNA-templated polymerases.

215 ped a method for the T4 DNA ligase-catalyzed DNA-templated polymerization of 5'-phosphorylated pentan

216 acids by using T4 DNA ligase to mediate the DNA-templated polymerization of 5'-phosphorylated trinuc

217 rogression on ultraviolet (UV) light-damaged DNA templates, possibly mediated by its ability to catal

218 l cases, fortuitous errors introduced during DNA template preparation and RNA transcription are suffi

219 detailed, step-by-step procedures, including DNA template preparation, in vitro and in vivo transcrip

220 sing xeno nucleic acid (XNA) polymerases, on DNA templates primed with DNA, RNA or XNA oligonucleotid

221 hCtf4 binds preferentially to DNA template-primer structures, interacts directly with

222 f RT polymerase activity with respect to the DNA template/primer (T/P), and consequently also inhibit

223 NA.dNTP complexes between MeFapy-dG-adducted DNA template:primer duplexes and the Y-family polymerase

224 The epigenetic regulation of DNA-templated processes has been intensely studied over

225 a central role in the epigenetic control of DNA-templated processes in eukaryotic cells.

226 c and epigenetic mechanisms, with nearly all DNA-templated processes influenced by chromatin architec

227 P) is considered to exert constraints to all DNA-templated processes, including base excision repair

228 Effector proteins influence DNA-templated processes, including transcription, DNA re

229 osome positioning can impact essentially all DNA-templated processes, making an appreciation of the f

230 post-translational modifications that alter DNA-templated processes, such as transcription, to facil

231 123ub1) plays a multifaceted role in diverse DNA-templated processes, yet the mechanistic details by

232 tion for signal transduction, affecting many DNA-templated processes.

233 estructures nucleosomes, is essential to all DNA-templated processes.

234 l and regulatory functions in all eukaryotic DNA-templated processes.

235 This naturally occurring DNA-templated reaction has the potential to generate cro

236 The user can customise DNA template/read length, the modelling of coverage base

237 mote replication fork progression on damaged DNA templates relies on its recently identified prolifer

238 , but how TLS polymerases gain access to the DNA template remains poorly understood.

239 nscription elongation factor that assists in DNA-templated RNA synthesis by cellular RNA polymerases

240 Specifically, DNA templates, RNA molecules, proteins and viral particl

241 cherichia coli selects its "codon-preferred" DNA template(s) for synthesis of proteins with required

242 s of 50 nm in size were produced from single DNA template sequence using a simple two step procedure

243 ng tunability of emission wavelength through DNA template sequence variations.

244 ivity is regulated by nascent RNA sequences, DNA template sequences, and conserved transcription fact

245 olymerization and pyrophosphate release with DNA templates showed that pyrophosphate (PPi) dissociati

246 DNA-templated silver nanoclusters are promising biologic

247 Ag K-edge EXAFS analysis of DNA-templated silver nanoclusters has been used to obtai

248 o the tuning of the fluorescence emission of DNA-templated silver nanoclusters.

249 sensing mechanism relies on building target DNA-templated silver nanowires (conductive paths) across

250 zymes through the in vitro selection of this DNA-templated small-molecule macrocycle library against

251 than those previously achieved by multistep DNA-templated small-molecule synthesis.

252 ymerase advances one nucleotide space on the DNA template strand after a correct nucleotide is incorp

253 We report that circularizing a DNA template strand encoding a pre-microRNA hairpin mimi

254 ctive center-proximal contacts stabilize the DNA template strand in the active center cleft and/or po

255 ial protein/DNA interactions that direct the DNA template strand into the RNAP active site.

256 We have previously developed a novel DNA template strand sequencing technique, called Strand-

257 quently, a dGh/dIa site was synthesized in a DNA template strand, and standing start primer extension

258 subunit caused by steric repulsion with the DNA template strand.

259 re identified during translocation of single DNA template strands through a modified Mycobacterium sm

260 se is inhibited by the presence of uracil in DNA template strands.

261 ich are brought together by hybridization to DNA template strands.

262 omoting translesion DNA synthesis as well as DNA template switching.

263 l strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an

264 together, these results establish the use of DNA-templated synthesis and in vitro selection to discov

265 A system for multistep DNA-templated synthesis is controlled by the sequential

266 We report the DNA-templated synthesis of Pd-Au bimetallic nanoparticle

267 rmined linear arrangement to a complementary DNA template that was chemoselectively modified with a h

268 generally contain a tract of adenines in the DNA template that yields a tract of uracils at the 3' en

269 Sequence elements in DNA templates that affect the yield of RNA and incorpora

270 DNA templates that contain 7dG in place of natural dG re

271 ccessful construction of a series of plasmid DNA templates that contain many tandem copies of one or

272 tive algorithm, the probability of selecting DNA templates that stabilize fluorescent silver clusters

273 e describe forward and reverse ratcheting of DNA templates through the alpha-hemolysin nanopore contr

274 9 DNA polymerase-controlled translocation of DNA templates through the M2MspA pore.

275 counterbalances the natural tendency of the DNA template to condense into toroids or buckle multiple

276 pproximately 2-fold) changes in the ratio of DNA template to nuclear extract were sufficient to chang

277 ed that allowed for the attachment of single DNA templates to gold nanoparticles with a single polyme

278 r proteins that enable transcription of both DNA templates.To identify the effector proteins, we tran

279 We integrated the DNA-templated translation system developed here into a c

280 Here, we report the development of a DNA-templated translation system that enables the enzyme

281 comprising a challenging methylated GC-rich DNA template under a novel 96-well microplate format.

282 We demonstrate that DNA templates up to 5000 nucleotides can be linearly amp

283 l resolution is limited by distortion of the DNA template upon Au metallization and subsequent etchin

284 eta-amino acid residues were translated from DNA templates using this strategy.

285 hich levels of virus oncogene expression per DNA template varied ~6.6-fold.

286 nteraction of nascent transcripts with their DNA template via the formation of co-transcriptional R-l

287 substrate charge, polymerization on a single DNA template was detected.

288 n and/or more than 50 ng of starting genomic DNA template was, however, detrimental to both the fract

289 n, with thermocycling and the use of a novel DNA template, we demonstrate a polymerase chain transcri

290 ry into CD34+, CD19+, and CD3+ cell subsets; DNA templates were prepared using quantitative polymeras

291 ly downstream motion of the enzyme along the DNA template, which has the effect of forward-biasing RN

292 can localize hyperflexible kinks within the DNA template, which in turn reduces the energetic cost t

293 /GCE that followed the shape produced by the DNA template, while the electrodeposition of NiONPs on t

294 When combined with a circular DNA template with a 5' unpaired flap, these proteins rec

295 tion of a linear or circular double-stranded DNA template with preassembled mushroom-shaped nanostruc

296 greater extent on an RNA template than on a DNA template with the same sequence.

297 e II (Pol II) molecules transcribing along a DNA template with two nucleosomes.

298 of a polymerase-bound 20,000-base-pair-long DNA template within seconds from a sub-nanogram input qu

299 oteins can be expressed directly from linear DNA templates within 90 min, eliminating the need for ad

300 ted molecular machinery would move along the DNA template without transient decondensation of observe