ライフサイエンスコーパス: DNAzyme

1 g of temporarily inactivated deoxyribozymes (DNAzymes).

2 ining aniline, H2O2, and a G-qudraplex-hemin DNAzyme.

3 and consequently the activity of peroxidase-DNAzyme.

4 ream activator after cleavage by an upstream DNAzyme.

5 triggered activation of the Mg(2+)-dependent DNAzyme.

6 lly relevant uranyl-binding sites in the 39E DNAzyme.

7 IL13 than cells from mice given the control DNAzyme.

8 of nanowires consisting of the HRP-mimicking DNAzyme.

9 deoxyuridine by UNG, however, activated the DNAzyme.

10 me-driven activation of the Mg(2+)-dependent DNAzyme.

11 t role in the catalytic function of the 8-17 DNAzyme.

12 reement with experiments on the related 8-17 DNAzyme.

13 ctrostatic interactions between ions and the DNAzyme.

14 sed on an in vitro-selected UO2(2+)-specific DNAzyme.

15 d well with the RNA cleavage activity of the DNAzyme.

16 nger RNA, compared with mice given a control DNAzyme.

17 lent transition metal dependent RNA-cleaving DNAzyme.

18 labeling a fluorophore and a quencher on the DNAzyme.

19 e demonstrate for the 10-23 RNA endonuclease DNAzyme.

20 Tm7 is an Er(3+)-dependent RNA-cleaving DNAzyme.

21 when complexed with hemin become functional DNAzymes.

22 s, aptamers, peptides, protein scaffolds and DNazymes.

23 dependent deactivation and activation of the DNAzymes.

24 shift in the CD spectra of the G-quadruplex DNAzymes.

25 ine-Hg(2+) interactions and Hg(2+)-activated DNAzymes.

26 logy, rolling circle amplification (RCA) and DNAzymes.

27 incorporating the PS modification to another DNAzyme, a sensor array was prepared to detect each meta

28 DNAzymes able to cleave the target ODC RNA were identifi

29 The present study demonstrates DNAzyme activation in the presence of metal ions (Pb(2+)

30 mer complexes remaining on the surface after DNAzyme activity can be greatly enhanced (down to one th

31 gh those monovalent ions that do not support DNAzyme activity have weaker binding affinity (K(d) appr

32 tivation method is presented for controlling DNAzyme activity in living cells.

33 ontrast to the hammerhead ribozyme, the 8-17 DNAzyme activity is not detectable in the presence of 4

34 or Rb(+) and Cs(+)), while those that confer DNAzyme activity possess stronger affinity (K(d) approxi

35 Irradiation at 365 nm restored DNAzyme activity, thus allowing the temporal control ove

36 -induced array assembly can be disrupted via DNAzyme activity.

37 8-17 DNAzyme adopts a V-shape fold, and the Pb(2+) cofactor i

38 The combination of PNA, RCA, and DNAzymes allows for sequence-specific and highly sensiti

39 Finally, the hemin/G-quadruplex DNAzyme/Amplex Red system was used to follow the activit

40 In addition, the cumulative nature of the DNAzyme-amplified signal generation process produced a d

41 turally occurring ribozymes, making the 8-17 DNAzyme an excellent choice as a Pb(2+) sensor with high

42 interaction between the active-conformation DNAzyme and a small molecule dye, N-methylmesoporphyrin

43 e two types of sensor methods using the same DNAzyme and AuNPs, making it possible to reveal advantag

44 m sensors based on uranyl (UO2(2+)) specific DNAzyme and gold nanoparticles (AuNP) have been develope

45 Colonic distribution of labeled DNAzyme and inflammation were monitored by in vivo imagi

46 NA chains consisting of the Mg(2+)-dependent DNAzyme and sequences that are complementary to the loop

47 sly reported the photochemical activation of DNAzymes and antisense agents through the preparation of

48 estigating the photochemical deactivation of DNAzymes and antisense agents.

49 ucleotides prohibits the formation of active DNAzymes and eliminates the release of the respective dy

50 ng a library of predesigned Mg(2+)-dependent DNAzymes and their respective substrates.

51 tic functions like those of protein enzymes (DNAzymes) and specific binding functions like antibodies

52 This is a new DNAzyme, and a catalytic beacon sensor is designed by at

53 mplementary sequence to the Mg(2+)-dependent DNAzyme, and a sequence identical to the loop region of

54 Using RNase digestion, DNAzyme, and RNA mobility shift assays, we demonstrate t

55 Our results showed that CRET-based DNAzyme-aptamer biosensing enabled specific OTA analysis

56 We have designed a single-stranded DNAzyme-aptamer sensor for homogeneous target molecular

57 ytic activities as compared to the separated DNAzyme/aptamer units, and the most active nucleoapzyme

58 e targets that are difficult to recognize by DNAzymes, aptamers, or antibodies, and without the need

59 uplex nanostructure and the Pb(2+)-dependent DNAzyme are implemented to develop sensitive surface pla

60 DNAzymes are a promising platform for metal ion detectio

61 ence of Mg(2+) or Zn(2+) ions the respective DNAzymes are activated, leading to the specific cleavage

62 DNAzymes are DNA-based catalysts; they typically recruit

63 DNAzymes are enzymatically active deoxyoligonucleotides

64 DNAzymes are known to bind metal ions specifically to ca

65 two decades ago, the metal-binding sites in DNAzymes are not fully understood.

66 While no Tl(3+)-dependent DNAzymes are obtained, a DNA oligonucleotide containing

67 RNA-cleaving DNAzymes are the catalytic DNAs discovered the earliest,

68 DNA strands with catalytic activity (DNAzymes) are an attractive alternative, enabling ration

69 Deoxyribozymes (DNAzymes) are single-stranded DNA that catalyze nucleic

70 alyzing DNA by the Zn(2+)-dependent ligation DNAzyme as amplifying biocatalyst is presented.

71 g the horseradish peroxidase (HRP)-mimicking DNAzyme as an amplifying label.

72 tch produces an active nucleic acid-cleaving DNAzyme as an output and this allows the switches to be

73 uplex horseradish peroxidase (HRP)-mimicking DNAzyme as catalytic labels that provide colorimetric or

74 as supporting matrix and hemin/G-quadruplex DNAzyme as signal amplifier for determination of hepatit

75 As uranyl is the cofactor of the 39E DNAzyme as well as the probe, specific uranyl binding ha

76 onment, making it the first demonstration of DNAzymes as intracellular metal ion sensors.

77 the peroxidase-mimicking DNAzyme (peroxidase-DNAzyme) as general and inexpensive platform for develop

78 resulting nanostructures bear split parts of DNAzyme at each end of the four arms which, in the prese

79 tionalization of a therapeutic mRNA-cleaving DNAzyme at the particle's surface.

80 ovel therapeutic inhaled GATA3 mRNA-specific DNAzyme attenuated early- and late-phase allergic respon

81 We demonstrate that this DNAzyme-AuNP probe can readily enter cells and can serve

82 ying enzymes and expand the analyte range of DNAzyme-based biosensors.

83 While DNAzyme-based metal sensors have found many applications

84 Furthermore, we demonstrate that this DNAzyme-based sensor can readily enter cells with the ai

85 ether, these results demonstrate that such a DNAzyme-based sensor provides a promising platform for d

86 DNAzyme-based sensors are highly attractive for their ex

87 platform for metal ion detection, and a few DNAzyme-based sensors have been reported to detect metal

88 The IC 3D integrates real-time, DNAzyme-based sensors, droplet microencapsulation and a

89 r range of analytes to take advantage of the DNAzyme-based signal amplification for more sensitive de

90 A DNAzyme beacon was engineered detecting down to 1.7 nM C

91 affinity interaction between the peroxidase-DNAzyme bearing hairpin sequence and the analyte (DNA or

92 In addition, drying of fully assembled DNAzyme before reaction with Pb(II) does not significant

93 wnian dynamics simulations, we find that the DNAzyme bends its substrate away from the cleavage point

94 The 5'-end of the DNAzyme binds the substrate DNA via Watson-Crick bonding

95 rovides a new route to obtain metal-specific DNAzymes by atomic replacement and also offers important

96 lar engineering to improve the properties of DNAzymes by designing a unimolecular probe for lead ion

97 detecting metal ions, as many metal-specific DNAzymes can be obtained using in vitro selection.

98 These catalytic DNAs, or DNAzymes, can be activated by metal ions.

99 The SCS can be activated by various upstream DNAzymes, can be coupled to DNA strand-displacement devi

100 ge of DNAzyme moieties from miRNA-hybridized DNAzyme-capped capture probes (DZ-CPs) from magnetic bea

101 l understanding of multivalent ion dependent DNAzyme catalysis.

102 mechanistic insights into metal binding and DNAzyme catalysis.

103 exposing the reactive site and buckling the DNAzyme catalytic core.

104 We also demonstrate that DNAzymes, catalytic DNA molecules, can be incorporated i

105 study describes the novel hemin/G-quadruplex DNAzyme-catalyzed aerobic oxidation of thiols to disulfi

106 Consequently, the CRET occurred between a DNAzyme-catalyzed chemiluminescence reaction and the que

107 The DNAzyme-catalyzed cleavage of a fluorophore/quencher-mod

108 hrough the fluorescence enhancement from the DNAzyme-catalyzed cleavage of DNA substrates labeled by

109 metric detection was carried out through the DNAzyme-catalyzed oxidation of 3,3',5,5'-tetramethylbenz

110 steine, glutathione) using the H2O2-mediated DNAzyme-catalyzed oxidation of Amplex Red to the resoruf

111 The mechanism of the reaction involves the DNAzyme-catalyzed oxidation of thiols to disulfides and

112 The DNAzyme catalyzes the polymerization of aniline and the

113 tidine C13 in the catalytic core of the same DNAzyme caused significant decrease of the activity.

114 An RNA-cleaving DNAzyme (Ce13d) was recently reported to be active with

115 thrombin-aptamer complex was found to block DNAzyme cleavage activity both in solution and in an ssD

116 arrays are explored with DNA hybridization, DNAzyme cleavage, and nuclease digestion experiments.

117 ligated product, and the resulting assembled DNAzyme cleaves a fluorophore/quencher-modified substrat

118 ion and in the presence of Na(+) , the NaA43 DNAzyme cleaves its substrate strand and releases a prod

119 In the presence of uranyl, the DNAzyme cleaves the fluorophore-labeled substrate strand

120 resence of Pb(2+) ions, the Pb(2+)-dependent DNAzyme cleaves the substrate, leading to the separation

121 rapid detection of ascorbic acid (AA) with a DNAzyme cleaving its DNA substrate in the presence of Cu

122 nto the system, and this hybridizes with the DNAzyme components and releases the ligated product for

123 diagnostics, we have developed a peroxidase DNAzyme construct that can be used as a chromogenic func

124 DNAzyme contains a loop forming a complex with Cu(2+) io

125 Here, we report three catalytic form 8-17 DNAzyme crystal structures.

126 t of the turnover number of the G-quadruplex DNAzyme; decomposition of G-quadruplex DNAzymes is slowe

127 ed controlled activation of the sensor after DNAzyme delivery into cells.

128 (DNAzyme-DPs) brought the target SNP and the DNAzyme-DPs onto the magnetic beads.

129 robes containing multiple DNAzyme sequences (DNAzyme-DPs) brought the target SNP and the DNAzyme-DPs

130 r presented by the Zn(2+)-dependent ligation DNAzyme-driven activation of the Mg(2+)-dependent DNAzym

131 These 6MI point mutant DNAzymes fall into three distinct functional classes, wh

132 A Pb(II)-specific DNAzyme fluorescent sensor has been modified with a thio

133 that, in the presence of Zn2+ and Mg2+, the DNAzyme folds into a compact structure, stem III approac

134 In addition to RNA transcription, DNAzyme footprinting can be coupled to a wide variety of

135 r detecting protein-aptamer complexation as "DNAzyme footprinting" in analogy to the process of DNase

136 ral control over the sensing activity of the DNAzyme for metal ions.

137 e same strategy was also applied to the GR-5 DNAzyme for the detection of Pb(II), thus demonstrating

138 icient, visible light-harnessing, photolyase DNAzymes for either the prophylaxis or therapy of UV dam

139 concentration of HRP-mimicking G-quardruplex DNAzyme formed from the binding interaction between hemi

140 DP), the hybridization of which prevents the DNAzyme from being active.

141 resence of uranyl induced disassembly of the DNAzyme functionalized AuNP aggregates, resulting in red

142 A sequences and structures such as aptamers, DNAzymes, G-quadruplexes, and i-motifs can be readily pr

143 FNAs mainly include DNAzymes, G-quadruplexes, and mismatched base pairs and

144 on of a polymerization/nicking machinery and DNAzyme generation path leads to an improved analysis of

145 Since the DNAzyme has been used as a metal ion sensor, its metal-i

146 significant promise, cellular sensing using DNAzymes has however been difficult, mainly because of t

147 Although a number of DNAzymes have been discovered by in vitro selection, the

148 lencing for disease treatments, RNA-cleaving DNAzymes have been extensively studied; however, the mec

149 DNAzymes have been previously used to detect divalent me

150 DNAzymes have enjoyed success as metal ion sensors outsi

151 DNAzymes have shown great promise as a general platform

152 A sequences (deoxyribozymes, DNA enzymes, or DNAzymes) have been identified by in vitro selection for

153 Although deoxyribozymes (DNAzymes) have been widely used as biosensors for the de

154 in its function, which may contribute to the DNAzyme having the highest activity in the presence of P

155 ation is unfavored and where the noncovalent DNAzyme-hemin complex has no activity.

156 The DNAzyme hgd40 inhibited expression of GATA3 messenger RN

157 outs); some mice were given a GATA3-specific DNAzyme (hgd40) or a control DNAzyme via intrarectal adm

158 The DNAzyme hybridization arms were altered from equal lengt

159 aptamers that employs the inhibition of the DNAzyme hydrolysis of aptamer monolayers is described.

160 the oxidative catalytic activity of a split DNAzyme in a highly controllable manner.

161 -fourths and one-fourth of the HRP-mimicking DNAzyme in caged, inactive configurations are used as fu

162 , the metal ion with higher affinity for the DNAzyme in global folding (Kd(Zn) = 52.6 microM and Kd(M

163 an assemble into active peroxidase-mimicking DNAzymes in the presence of bioanalytes such as DNA, the

164 by 2'-O-nitrobenzyl adenosine, rendering the DNAzyme inactive and thus allowing its delivery into cel

165 c determination system based on G-quadruplex DNAzyme integrated with a smartphone was developed to qu

166 0.1 min(-1)], and the transformation of this DNAzyme into a fluorescent sensor for Na(+) by labeling

167 The 8-17 DNAzyme is a DNA metalloenzyme catalyzing RNA transester

168 The immobilized DNAzyme is a robust system; it may be regenerated after

169 Self-assembly of the DNAzyme is accomplished by first adsorbing the single-th

170 The DNAzyme is based on a reported 18-mer G-quadruplex-formi

171 This DNAzyme is highly selective for lanthanides as well, sho

172 The Zn(2+)-dependent ligation DNAzyme is implemented as a biocatalyst for the amplifie

173 he reactivity and global folding of the 8-17 DNAzyme is investigated, and the results are compared wi

174 hybridization with an oligonucleotide-tailed DNAzyme is performed to introduce the DNAzyme to the bio

175 his work, in vitro selection of RNA-cleaving DNAzymes is carried out using Tl(3+) as the target metal

176 The catalytic activity of the DNAzymes is restored in a universal manner in response t

177 uplex DNAzyme; decomposition of G-quadruplex DNAzymes is slower in buffers that contain ammonium ions

178 ve the catalytic functions of nucleic acids (DNAzymes) is introduced.

179 autonomous synthesis of the Mg(2+)-dependent DNAzyme, is used for the amplified, multiplexed analysis

180 point for interpreting experimental data on DNAzyme kinetics, as well as developing more detailed si

181 dition of hemin to antiparallel G-quadruplex DNAzymes lead to a blue shift in the CD spectra of the G

182 encher-modified substrates by the respective DNAzymes leads to the fluorescence of F1 and/or F2 as re

183 uence (corresponding to the substrate of the DNAzyme) linked to a G-rich domain, which is "caged" in

184 ization to surface-bound capture probes, the DNAzyme-linked LCR products induce electrocatalytic resp

185 anticancer drug, doxorubicin, by the Mg(2+)-DNAzyme-locked pores or by the aptamer-ATP complex-trigg

186 ptasensors using replication/nicking enzymes/DNAzyme machineries is described.

187 These results indicate that this DNAzyme may be a useful tool to study the function of OD

188 suggest that for Pb2+ global folding of the DNAzyme may not be a necessary step in its function, whi

189 y phosphoryl transfer from [gamma-(32)P]GTP, DNAzyme-mediated cleavage yielded two radiolabeled cleav

190 This DNAzyme might be developed for treatment of patients wit

191 It is based on the cleavage of DNAzyme moieties from miRNA-hybridized DNAzyme-capped ca

192 target miRNA is realized through the cleaved DNAzyme moieties-catalyzed oxidation of 3,3',5,5'-tetram

193 These aptamers contain a DNAzyme moiety that is initially maintained in an inacti

194 Control experiments using two 39E DNAzyme mutants revealed a different cleavage pattern of

195 in, we report a silver-specific RNA-cleaving DNAzyme named Ag10c obtained after six rounds of in vitr

196 We previously reported a DNAzyme named Ce13d, which has similar responses to all

197 Among them, a DNAzyme named PSCu10 was studied further.

198 a Ce(4+) salt as the target metal, and a new DNAzyme (named Ce13) with a bulged hairpin structure was

199 One of the representative DNAzymes (named Lu12) was further studied.

200 ctivity, these results suggest that the 8-17 DNAzyme, obtained by in vitro selections, has evolved to

201 vities, indicating the global folding of the DNAzyme occurs before the cleavage activity for those me

202 cludes the horseradish peroxidase (HRP)-like DNAzyme, optimum-length linker (10-mer-length DNA), and

203 rough either metal ion-dependent cleavage by DNAzymes or analyte-dependent structural-switching by ap

204 elected position in the DNA duplex region of DNAzymes or aptamers.

205 suitable functional-DNA partners (aptamers, DNAzymes or aptazymes).

206 e Toffoli and Fredkin gates by the action of DNAzymes or the use of a multifluorophoric platform as a

207 nput target can produce more than one active DNAzyme output.

208 nstrates the use of the peroxidase-mimicking DNAzyme (peroxidase-DNAzyme) as general and inexpensive

209 reaction, hemin-binding peroxidase-mimicking DNAzymes ("peroxidymes") mediate the NADH-driven oxidati

210 can allow for the discovery of a ribozyme or DNAzyme phenotype that would not likely be encountered b

211 sed of glucose oxidase (GOx) and pistol-like DNAzyme (PLDz) to detect glucose levels in tears and sal

212 (ii) The DNAzyme-powered logic gates are made to operate at a fin

213 The system consists of a three-stranded DNAzyme precursor (TSDP), the hybridization of which pre

214 eviously been designed that are propelled by DNAzymes, protein enzymes and strand displacement.

215 In addition, induction of DNAzyme provides a new approach in the development of gl

216 ork enables the rational design of synthetic DNAzyme regulatory networks, with potential applications

217 The metal ion-driven DNAzyme release of substrates from the pores of MP-SiO(2

218 uranyl resulted in cleavage of substrate by DNAzyme, releasing a single stranded DNA that can be ads

219 gle guanine residues within UV1C extends the DNAzyme's activity into the violet region of the spectru

220 yuridine, resulting in minimal change of the DNAzyme's activity.

221 Precise regulation of the DNAzyme's oxidative catalysis can be achieved by externa

222 he detection of other metal ions using other DNAzymes selected through in vitro selection.

223 environment, no intracellular application of DNAzyme sensors has yet been reported.

224 of the "always-on" mode of first-generation DNAzyme sensors.

225 A complex consisting of the Pb(2+)-dependent DNAzyme sequence and a ribonuclease-containing nucleic a

226 chemes by integrating a peroxidase-mimicking DNAzyme sequence into the LCR amplification probes desig

227 th a fluorophore is linked to a hairpin 8-17 DNAzyme sequence labeled with a quencher.

228 CPs and detection probes containing multiple DNAzyme sequences (DNAzyme-DPs) brought the target SNP a

229 sequences for two different Mg(2+)-dependent DNAzyme sequences and two different functional hairpin s

230 domains and the respective Mg(2+)-dependent DNAzyme sequences are implemented as nicking/replication

231 p domains of the Mg(2+)- or Zn(2+)-dependent DNAzyme sequences with foreign nucleotides prohibits the

232 synthesis of two different Mg(2+)-dependent DNAzyme sequences.

233 Nt.BbvCI and two different Mg(2+)-dependent DNAzyme sequences.

234 onsisting of the Mg(2+)- or Zn(2+)-dependent DNAzyme sequences.

235 Surprisingly, the selected Zn(2+)-dependent DNAzymes showed only a modest (approximately 3-fold) act

236 Multi-layered DNAzyme signaling and logic cascades are now reported.

237 emin/G-quadruplex structure as HRP mimicking-DNAzyme significantly improved the catalytic reduction o

238 So far, no RNA-cleaving DNAzymes specific for Cu(2+) are known.

239 n the presence of the analyte the peroxidase-DNAzyme structure is disrupted and does not catalyze the

240 which induced the quencher dye close to the DNAzyme structure.

241 mation of active Mg(2+)- or Zn(2+)-dependent DNAzyme structures through the cooperative formation of

242 ntal observations concerning the size of the DNAzyme/substrate complex, the impact of the recognition

243 cording to this method, the Mg(2+)-dependent DNAzyme subunits displace the ligated product, and the r

244 dergo a structural switch that activates the DNAzyme, such that the binding event can be reported thr

245 lts in the formation of the Mg(2+)-dependent DNAzyme tethered to a free strand consisting of the targ

246 s and the displacement of a Mg(2+)-dependent DNAzyme that catalyzes the generation of a fluorophore-l

247 on of a trigger RNA into the production of a DNAzyme that degrades an independent RNA substrate, a si

248 A DNAzyme that prevents expression of GATA3 reduces coliti

249 ionally, there are specific sequences called DNAzymes that can fold into tertiary structures that dis

250 cted and quantified by deoxyuridine-modified DNAzymes that underwent UNG-dependent deactivation or ac

251 y and efficacy of SB010, a novel DNA enzyme (DNAzyme) that is able to cleave and inactivate GATA3 mes

252 Another DNAzyme, the 8-17 DNAzyme, which has a similar secondary

253 Taking advantages of smartphone and DNAzyme, the assay provides great potential for its prac

254 his PS-modified oligonucleotide with the Tm7 DNAzyme, the cleavage yield increases to approximately 4

255 fficient catalytic activity of HRP mimicking-DNAzyme, the proposed immunosensor exhibited high sensit

256 articularly the allosteric activation of the DNAzymes through cooperative aptamer-substrate complexes

257 By monitoring the activity change of the DNAzymes through the fluorescence enhancement from the D

258 es allosterically stabilize and activate the DNAzymes, thus allowing the selective release of the flu

259 veal the modus operandi of the original UV1C DNAzyme to be a surprisingly versatile one.

260 y the signal from photocaged Na(+) -specific DNAzyme to detect endogenous Na(+) inside cells is repor

261 The binding strength of the DNAzyme to the aptamer sequence was designed to be less

262 tailed DNAzyme is performed to introduce the DNAzyme to the biosensor.

263 on to block site-specific cleavage of RNA by DNAzymes to show that MRM1, MRM2, and RNMTL1 are respons

264 The DNAzyme triggers the polymerization of 3,3'-dimethoxyben

265 The catalytic activity of each DNAzyme unit leads to colorimetric detection and provide

266 Different conjugation modes of the aptamer/DNAzyme units and the availability of different aptamer

267 porter units enhance the formation of active DNAzyme units, thus leading to the isothermal autocataly

268 CA-induced synthesis of the Mg(2+)-dependent DNAzyme units.

269 rate, we find that the catalytic core of the DNAzyme unwinds and the overall complex rapidly extends,

270 An approach to develop an effective DNAzyme, using the 10-23 model, against ODC is described

271 This method has expanded the DNAzyme versatility for detecting metal ions in biologic

272 GATA3-specific DNAzyme (hgd40) or a control DNAzyme via intrarectal administration, or systemic inje

273 In the labeled method, a uranyl-specific DNAzyme was attached to AuNP, forming purple aggregates.

274 ingle- or double-stranded DNAs, the modified DNAzyme was deactivated when the uracil at the indispens

275 mitation, a photoactivatable (or photocaged) DNAzyme was designed and synthesized, and its applicatio

276 A 28-base DNAzyme was designed to specifically bind to and cleave

277 leotide at the scissile position of the 8-17 DNAzyme was replaced by 2'-O-nitrobenzyl adenosine, rend

278 the indispensable thymidine T2.1 in the 8-17 DNAzyme was replaced with a deoxyuridine, resulting in m

279 Signaling between DNAzymes was achieved using a structured chimeric substr

280 problem, in vitro selection of RNA-cleaving DNAzymes was carried out using a library containing a re

281 subsequently the formation of HRP-mimicking DNAzymes was stimulated by adding hemin molecules.

282 Despite many studies since DNAzymes were discovered nearly two decades ago, the met

283 , and a few new Cu(2+)-specific RNA-cleaving DNAzymes were isolated.

284 itches that consist of nucleic-acid cleaving DNAzymes which are temporarily inactivated by hybridizat

285 We focus here on the well-studied 10-23 DNAzyme, which cleaves mRNA with a catalytic efficiency

286 Another DNAzyme, the 8-17 DNAzyme, which has a similar secondary structure but sho

287 dehybridizes the TSDP to release the active DNAzyme, which then carries out metal-ion-dependent clea

288 n a sample, by exposing a hemin/G-quadruplex DNAzyme, which then catalyzes the generation of chemilum

289 DNAzymes, which are sequences of DNA with catalytic acti

290 a shell consisting of a uranyl-specific 39E DNAzyme whose enzyme strand contains a thiol at the 3' e

291 ting the cleavage site of the Na(+)-specific DNAzyme with a photolabile o-nitrobenzyl group, we achie

292 obe specific uranyl-binding sites in the 39E DNAzyme with catalytically relevant concentrations of ur

293 (2+) and the cleavage of a substrate by 8-17 DNAzyme with Pb(2+) in solution, as well as sensitive DN

294 er (FRET) by labeling the three stems of the DNAzyme with the Cy3/Cy5 FRET pair two stems at a time i

295 o selection of Zn(2+)-dependent RNA-cleaving DNAzymes with activity at 90 degrees C has yielded a div

296 a few Cu(2+) biosensors were reported using DNAzymes with DNA cleavage or DNA ligation activity.

297 of hemin, form catalytic hemin/G-quadruplex DNAzymes with peroxidase activity.

298 Na(+)-specific, RNA-cleaving deoxyribozyme (DNAzyme) with a fast catalytic rate [observed rate const

299 ognition sequence (aptamer) to the catalytic DNAzyme, yielding a hybrid structure termed "nucleoapzym

300 of hemin into the G-quadruplex structure of DNAzyme yields an active HRP-like activity that catalyze