ライフサイエンスコーパス: DNMT1

1 m infancy onward by DNA methyltransferase 1 (DNMT1).

2 in cooperation with DNA methyltransferase 1 (DNMT1).

3 DNA methylation by DNA methyltransferase 1 (DNMT1).

4 enance DNA (cytosine-5) methyltransferase 1 (DNMT1).

5 ast two of its known targets, namely ID4 and DNMT1.

6 ent degradation of the DNA methyltransferase DNMT1.

7 tate (TS) of the reaction catalyzed by human DNMT1.

8 ransfer step is chemically rate-limiting for DNMT1.

9 HDAC1 and DNA methyl transferases DNMT3b and DNMT1.

10 ol the recruitment and enzymatic activity of DNMT1.

11 lling is maintained by DNA methyltransferase DNMT1.

12 against a possible excessive methylation by DNMT1.

13 iption, including the DNA methyltransferase, DNMT1.

14 (RG108) was first identified as inhibitor of DNMT1.

15 ccumulation of H4K20me3 and the clearance of DNMT1.

16 tics and RNA inhibition of recombinant human DNMT1.

17 s accelerated the proteasomal degradation of DNMT1.

18 ceptor P2RY11, and the DNA methyltransferase DNMT1.

19 ing pathways mediating the flow-induction of DNMT1.

20 of microRNA 148a (miR-148a), which represses DNMT1.

21 DNMT3A and DNMT3B and the maintenance enzyme DNMT1.

22 S-phase by the maintenance methyltransferase Dnmt1.

23 e genome that was rescued by transfection of DNMT1.

24 Np95 is deleted alone or in combination with Dnmt1.

25 cing the expression of DNA methyltransferase DNMT1, -2, -3A, and -3B alone by siRNA did not affect NP

26 miR-185 overexpression inhibited DNMT1 3' untranslated region luciferase reporter activit

27 ing is mediated by DNA methyltransferase1/3 (DNMT1/3)-, histone deacetylase 1/2/4 (HDAC1/2/4)-, Setdb

28 Human DNA methyltransferase 1 (Dnmt1), a 1616-amino acid multidomain enzyme, is essenti

29 However, while IAPs are derepressed in Dnmt1-ablated embryos and embryonic stem cells (ESCs), t

30 ons as well as implicating RNA in regulating DNMT1 activity and correct levels of genomic methylation

31 Dnmt1 activity is independent of the presence of Dnmt3a

32 Based on differences in DNMT1 activity measured with this assay, we distinguish

33 led gDMD-like sequences requiring continuous DNMT1 activity to sustain a highly methylated state.

34 istry to measure selectively and sensitively DNMT1 activity within these complex and congested cellul

35 ed in aberrant DNA methylation by inhibiting DNMT1 activity, resulting in altered gene expression.

36 rocessing of RNA is necessary to ensure full DNMT1 activity.

37 Tgbr2 had elevated DNA methyltransferase I (DNMT1) activity and histone H3 lysine 9 trimethylation (

38 BM gamma-H2AX (DNA repair/damage marker) and DNMT1 analyses.

39 However, the simultaneous attenuation of DNMT1 and -3B expression caused a significant increase i

40 ted for the DNA methyltransferases 1 and 3B (DNMT1 and 3B) or by DNMT inhibitors (DNMTi).

41 ish hepatocytes destabilizes and delocalizes Dnmt1 and causes DNA hypomethylation and Tp53-mediated s

42 tformin post FAE affect memory via elevating Dnmt1 and consequently normalizing hippocampal Dio3 and

43 n mice, reminiscent of a genetic knockout of Dnmt1 and could substantially suppress intestinal polyp

44 e maintenance cytosine DNA methyltransferase DNMT1 and de novo methyltransferase DNMT3b cooperate to

45 astrulation stage; however, the functions of Dnmt1 and DNA methylation in organogenesis remain unclea

46 of TGF-beta1 to increase expression of both DNMT1 and DNMT3a demonstrates a novel means by which TGF

47 trate that TGF-beta1 increases expression of DNMT1 and DNMT3a through different post-transcriptional

48 The increases in DNMT1 and DNMT3a were dependent on TGF-beta1 activation

49 tein expression, but not RNA levels, of both DNMT1 and DNMT3a.

50 We show that DNMT1 and DNMT3a/3b activities work complementarily and

51 erve an unexpected division of labor between DNMT1 and DNMT3a/3b in suppressing retrotransposon long

52 catalytically active DNA methyltransferases (DNMT1 and DNMT3A/B) require accessory proteins such as U

53 is of functional importance to induction of DNMT1 and DNMT3b and, in turn, changes in DNA methylatio

54 These results demonstrate that Dnmt1 and Dnmt3b cooperate to maintain DNA methylation a

55 Ablation of both Dnmt1 and Dnmt3b in the intestinal epithelium is lethal,

56 We show that MUC1-C occupies the DNMT1 and DNMT3b promoters in complexes with NF-kappaB p

57 Elevated recruitment of Dnmt1 and Dnmt3b to the Ogg1 promoter in acrolein treate

58 s in complexes with NF-kappaB p65 and drives DNMT1 and DNMT3b transcription.

59 strate that MUC1-C induces the expression of DNMT1 and DNMT3b, but not DNMT3a, in breast and other ca

60 n of Elongin A with and transcription of the DNMT1 and EFR3A genes without a decrease in Pol II recru

61 Knockdown of DNMT1 and EP300 in L6-S2 DRG neurons of rats reduced DNA

62 We demonstrated that DNMT1 and KIT form a positive regulatory loop, in which

63 Overexpression of DNMT1 and KIT is prevalent in lung cancer, yet the under

64 in lung cancer pathogenesis, whether and how DNMT1 and KIT orchestrate lung tumorigenesis are unclear

65 cer cells, with reciprocal downregulation of DNMT1 and MEK/ERK phosphorylation.

66 Conditional deletions reveal that both Dnmt1 and Np95 are essential for maintenance DNA methyla

67 e extensively survey diverse species lacking Dnmt1 and show that, surprisingly, symmetrical CG methyl

68 s required for Tip60-mediated acetylation of Dnmt1 and subsequent Dnmt1 ubiquitylation and degradatio

69 ined 2 small molecules-decitabine to deplete DNMT1 and tetrahydrouridine (THU) to inhibit cytidine de

70 functions as a scaffolding protein for both Dnmt1 and Tip60 and is required for Tip60-mediated acety

71 d the up-regulation of DNA methyltransferase DNMT1 and tyrosine-protein kinase KIT, the enhanced phos

72 Disruption of the interaction between DNMT1 and UHRF1 by replacement of key residues in the re

73 an up-regulation of DNA methyltransferase 1 (DNMT1) and a global hypermethylation in vascular endothe

74 MDR]) that recruits DNA methyltransferase 1 (Dnmt1) and provokes methylation of the Gata1 gene enhanc

75 , are replicated by DNA methyltransferase 1 (DNMT1) and ubiquitin-like containing PHD and RING finger

76 uhrf1 mutants are phenocopied by mutation of dnmt1, and Dnmt1 knockdown in uhrf1 mutants enhances the

77 plication focus targeting sequence domain of DNMT1, and it does not require DNA binding by the SRA do

78 s revealed the association of HDAC1, DNMT3b, DNMT1, and mSin3A with SNAIL.

79 Moreover, tumour EZH2 and DNMT1 are negatively associated with tumour-infiltrating

80 These findings establish DNMT1 as a bona fide target of miR-185 in HCC cells.

81 and in vivo tumorigenic potential, defining DNMT1 as a candidate CSC therapeutic target.

82 f proteins involved in this process, such as DNMT1, as a new crucial role of the SCMC for mammalian r

83 cRNA, TCONS_00023265, which we named DACOR1 (DNMT1-associated Colon Cancer Repressed lncRNA 1), shows

84 our results demonstrate that deregulation of DNMT1-associated lncRNAs contributes to aberrant DNA met

85 ss also was associated with up-regulation in DNMT1-associated methylation of the Cnr1 promoter and do

86 DNMT1-associated protein 1 (DMAP1) is a member of the TI

87 de from its role in the CXXC domain-mediated DNMT1 autoinhibition, serves as an important regulatory

88 nd HepG2 hepatoblastoma cells expressing low DNMT1 basal levels also possessed a high self-renewal, i

89 Lowering DNMT1 below a threshold level is required for maximal lo

90 a dominant role for DNA methyltransferase 1 (DNMT1) but also distinct roles of 3A and 3B in genome-wi

91 te that UHRF1 also increases the activity of DNMT1 by almost 5-fold.

92 Transcriptional repression of Dnmt1 by REMOTE-control was potent enough to cause embry

93 In contrast, the increase in DNMT1 by TGF-beta1 was not dependent on new protein synt

94 trolled in part by ACL and that silencing of DNMT1 can accelerate adipocyte differentiation.

95 Deficiency of Dnmt1 causes severe intestinal abnormalities in neonates

96 Functionally, KIT and DNMT1 co-expression promotes, whereas dual inactivation

97 how that endogenous DNA methyltransferase 1 (DNMT1) co-purifies with inhibitory ncRNAs.

98 We generated Dnmt1 conditional knockout mice (Dnmt1(Deltaalb) ) by cr

99 DNMT1 contains, in addition to a C-terminal methyltransf

100 These were properties shared by MLL and DNMT1 CXXC domains but not by CGBP CXXC or the other swa

101 lls, thus representing a novel antagonist of DNMT1 degradation.

102 Acute Dnmt1 deletion elicits dramatic hypomethylation and geno

103 mammary tumours, and mammary gland-specific DNMT1 deletion protects mice from mammary tumorigenesis

104 sion of four genes, DNA methyltransferase 1 (DNMT1), delta-opioid receptor (OPRD1), cannabinoid recep

105 e generated Dnmt1 conditional knockout mice (Dnmt1(Deltaalb) ) by crossing Dnmt1(fl/fl) with albumin-

106 in postnatal liver growth and regeneration; Dnmt1(Deltaalb) mice provide a unique experimental model

107 The Dnmt1(Deltaalb) phenotype was assessed by histology, con

108 Understanding the TS of DNMT1 demonstrates the possibility of using similar anal

109 The finding of distinct roles of DNMT1-dependent and -independent methylation patterns in

110 ction and induces atherosclerosis in an mTOR/DNMT1-dependent manner.

111 DNMT1 expression increases, whereas targeted DNMT1 depletion abrogates KIT signaling cascade through

112 enerations, confirming the essential role of DNMT1 depletion in the enhancement of cancer stem cell p

113 Noncytotoxic DNMT1 depletion was confirmed by serial BM gamma-H2AX (D

114 cytotoxicity and increase exposure time for DNMT1 depletion, to safely and effectively circumvent mu

115 ure, which is required for S-phase-dependent DNMT1 depletion.

116 As expected with non-cytotoxic DNMT1-depletion, platelets increased and neutrophils con

117 Dali interacts with the DNMT1 DNA methyltransferase in mouse and human and regul

118 DNMT1 (DNA methyltransferase 1) is responsible for propa

119 for 11 SNPs within DNA methyltransferase 1 (DNMT1), DNA methyltransferase 3 Beta (DNMT3B), Tet methy

120 HF20L1 binds to monomethylated lysine 142 on DNMT1 (DNMT1K142me1) and colocalizes at the perinucleola

121 et single (Mecp2) as well as multiple genes (Dnmt1, Dnmt3a and Dnmt3b) in the adult mouse brain in vi

122 of SAHH, SAH, DNA methyltransferases genes (Dnmt1, Dnmt3a, Dnmt3b), and global DNA methylation in th

123 genetic regulators (e.g., Foxo3, Plk1, Mycn, Dnmt1, Dnmt3b, and Tet3).

124 that miR-342 regulates DNMT1 expression but DNMT1 does not affect the EVL expression in these cells.

125 -miR-148a-dependent mechanism for regulating DNMT1 during adipogenesis.

126 We further confirm the crucial role of Dnmt1 during crypt development using the in vitro organo

127 tion of MBD3 and MBD2 would co-localize with DNMT1 during DNA maintenance methylation, providing a pr

128 These patterns are faithfully maintained by DNMT1 during DNA replication to ensure epigenetic inheri

129 osphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication.

130 4B) through promoter demethylation; in turn, DNMT1 dysfunction impairs KIT kinase signaling.

131 , such as miR-155-5p, leads to inhibition of DNMT1 enzyme activity.

132 tion of selective chemical inhibitors of the DNMT1 enzyme.

133 Dnmt1 epigenetically propagates symmetrical CG methylati

134 Kras(G12D)-induced senescence by regulating Dnmt1 expression and consequently genome-wide levels of

135 xpression, suggesting that miR-342 regulates DNMT1 expression but DNMT1 does not affect the EVL expre

136 ding that RAS/MEK/ERK pathway activation and DNMT1 expression correlated with cytotoxic activity.

137 a positive regulatory loop, in which ectopic DNMT1 expression increases, whereas targeted DNMT1 deple

138 Furthermore, we find that DNMT1 expression is elevated in mammary tumours, and mam

139 that induction of intracellular HSP72 alters DNMT1 expression, and may function as an epigenetic regu

140 oxycytidine as well as by down-modulation of Dnmt1 expression, further supporting the role of DNA met

141 These effects persisted after restoration of DNMT1 expression.

142 d oncogenic transformation and regulation of Dnmt1 expression.

143 increased vascular DNA methyltransferase 1 (DNMT1) expression and that HS prevented this increase.

144 knockout mice (Dnmt1(Deltaalb) ) by crossing Dnmt1(fl/fl) with albumin-cyclization recombination tran

145 tion with UHRF1 increased the specificity of DNMT1 for methylation of hemimethylated CpG sites.

146 gene-body hypermethylation, dissociation of DNMT1 from the promoter, and VAV1 expression via SMAD4 a

147 h the epigenetic machinery especially UHRF1, DNMT1, G9a and the transcription factor Snail1.

148 fferent point mutations affecting exon 21 of DNMT1 gene in patients with ADCA-DN, a novel heterozygou

149 n mouse embryonic stem cells that endogenous Dnmt1 gene transcription could be up- or downregulated i

150 the hereditary motor and sensory neuropathy DNMT1 gene, highlighting the genetic heterogeneity of sp

151 Patients underwent genetic analysis of DNMT1 gene, neurophysiological tests investigating sleep

152 omains 1 (uhrf1) or DNA methyltransferase 1 (dnmt1) genes exhibit a robust interferon induction chara

153 direction of these associations depended on DNMT1 genotypes.

154 that interact with the DNA methyltransferase DNMT1 in a colon cancer cell line, HCT116.

155 cyclization recombination-driven deletion of Dnmt1 in adult quiescent hepatocytes did not affect live

156 d ligation surgery in a murine model induced DNMT1 in arterial endothelium.

157 ads and controls enzyme levels of methylated DNMT1 in cells, thus representing a novel antagonist of

158 Pharmacologic or genetic targeting of DNMT1 in CSCs reduced their self-renewal and in vivo tum

159 Consistently, efficient knockdown of Dnmt1 in cultured hepatic progenitor cells caused severe

160 Furthermore, genetic deletion of Dnmt1 in HSPCs activated Gata1 expression and depleted H

161 ustrate a clear differential requirement for Dnmt1 in immature versus mature organoids.

162 Loss of Dnmt1 in intervillus progenitor cells causes global hypo

163 se results demonstrate an essential role for Dnmt1 in maintaining genomic stability during intestinal

164 r, our studies uncover an essential role for DNMT1 in MaSC and CSC maintenance and identify DNMT1-ISL

165 The role of DNMT1 in miR-185-induced inhibition of HCC growth was fu

166 The data suggest a role for DNMT1 in modulating the timing of differentiation and de

167 Ablation of Dnmt1 in mouse embryos causes death at the post-gastrula

168 entify functional cross-talk between KIT and DNMT1 in the development of drug resistance, implying th

169 We investigated the role of DNMT1 in the regulation of postnatal liver histogenesis

170 that CDK4 interacted with and phosphorylated DNMT1 in vitro, suggesting that CDK activity is required

171 nds were tested for their ability to inhibit DNMT1 in vitro.

172 ased expression of the DNA methyltransferase DNMT1 in WDLS/DDLS.

173 downregulation of the DNA methyltransferase DNMT1 induced by the brain microenvironment-derived clus

174 te loss of the maintenance methyltransferase Dnmt1 induces widespread DNA demethylation and transcrip

175 DNMT1 inhibition, but not Polycomb inhibition, in RUNX1-

176 DS AND To pharmacologically re-induce HbF by DNMT1 inhibition, this first-in-human clinical trial (NC

177 nhibitors and small interfering RNAs and the Dnmt1 inhibitor markedly reduced H3K9me3 (49-79%), H3K27

178 Administering Dnmt1 inhibitor to control neonates resulted in FAE-like

179 monolayer at various cell densities with the DNMT1 inhibitor zebularine (ZEB) followed by a 3D cultur

180 ncentrations of the DNA methyltransferase 1 (DNMT1) inhibitor decitabine produce p53-independent cell

181 eoxycytidine (Aza), a DNA methyltransferase (DNMT1) inhibitor, reduces high blood pressure (BP) by re

182 doxycycline-mediated repression of exogenous DNMT1* initiates rapid, global loss of DNA methylation,

183 y Microprocessor component DROSHA as a novel DNMT1-interactor.

184 The proposed catalytic mechanism of DNMT1 involves nucleophilic attack of Cys(1226) to cytos

185 transcriptional activation in vivo, and that Dnmt1 is a critical regulator of postnatal epigenetic ch

186 Because 5-methylcytosine methyltransferase DNMT1 is also a potential target of miR-342, we inhibite

187 Dnmt1 is critical for immediate postnatal intestinal dev

188 Here, we report that Dnmt1 is crucial during perinatal intestinal development

189 Here we show that DNMT1 is indispensable for MaSC maintenance.

190 Lysine 142 of DNMT1 is methylated by the SET domain containing lysine

191 ncogenic cellular transformation by Ras, and Dnmt1 is overexpressed in numerous human cancers.

192 tissues, we found that the overexpression of DNMT1 is positively correlated with the upregulation of

193 During this process the augmentation of DNMT1 is regulated through at least two post-transcripti

194 DNA methyltransferase 1 (Dnmt1) is a key enzyme involved in the somatic inheritan

195 DNA methyltransferase 1 (DNMT1) is an essential regulator maintaining both epigen

196 malian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation.

197 suppressor genes by DNA methyltransferase 1 (Dnmt1) is essential for oncogenic cellular transformatio

198 protein, the essential targeting factor for DNMT1, is reduced upon transition to 2i, and so is recru

199 MT1 in MaSC and CSC maintenance and identify DNMT1-ISL1 axis as a potential therapeutic target for br

200 to facilitate the association of UHRF1 with DNMT1, its absence did not impair the loading of DNMT1 o

201 Conditional Dnmt1 knockdown in early eye development mediated by Rx-

202 ts are phenocopied by mutation of dnmt1, and Dnmt1 knockdown in uhrf1 mutants enhances their small li

203 Further, DNMT1 knockdown sensitized PKC412(R) cells to PKC412; co

204 The impact of Dnmt1 knockdown was also analyzed in hepatic progenitor

205 ption is reduced in DNA methyltransferase I (Dnmt1) knockout embryonic stem cells as well as in fibro

206 iR-185 inhibits HCC cell growth by targeting DNMT1, leading to PTEN induction and Akt inhibition.

207 Later in differentiation, DNMT1 levels decline in an ACL-dependent manner.

208 Mechanistically, CSC expressed higher DNMT1 levels than non-CSC.

209 udy, we report that DNA methyltransferase 1 (DNMT1) levels in adipocytes are controlled in part by AC

210 UV39 histone methyltransferase, DIM-5, and a DNMT1-like cytosine methyltransferase, DIM-2.

211 Dnmt1 loss in postnatal hepatocytes caused global hypome

212 omised in vivo in a mouse model of transient DNMT1 loss in the preimplantation embryo.

213 The DNA methyltransferase Dnmt1 maintains DNA methylation patterns and genomic sta

214 Human DNA methyltransferase 1 (DNMT1) maintains the epigenetic state of DNA by replicat

215 The regulatory domains of DNMT1 mediate a network of protein-protein and protein-D

216 tion (H3K27me3) and DNA methyltransferase 1 (DNMT1)-mediated DNA methylation repress the tumour produ

217 protein expression leading to inhibition of Dnmt1-mediated anti-apoptotic activity.

218 results establish the indispensable role of DNMT1-mediated epigenetic regulation in postnatal liver

219 processed by Microprocessor and can inhibit DNMT1-mediated methylation in vitro.

220 DNMT1-mediated methyltransferase activity is also reduce

221 e two extreme phenotypic manifestations of a DNMT1 methylopathy.

222 of human DNA(cytosine-5)-methyltransferase1 (DNMT1) methyltransferase activity, enabling measurements

223 etection of both bacterial (SssI) and human (Dnmt1) methyltransferase activity.

224 Aza treatment decreased the expression of DNMT1, MMP9, TIMP1, and S-adenosyl homocysteine hydrolas

225 TCR signal suppresses miR-148a to derepress DNMT1 mRNA translation.

226 rescue line and readily obtained homozygous DNMT1-mutant lines.

227 RNA-seq analysis of uhrf1 and dnmt1 mutants revealed widespread induction of Class I r

228 ated families carrying dominant heterozygous DNMT1 mutations linked to two distinct autosomal dominan

229 hogenic mechanism for DNA methyltransferase (DNMT1) mutations.

230 There is elevated expression of DNMT1, Notch1, and the viral gene product E1insertion ma

231 ACL-mediated suppression of DNMT1 occurs at least in part by promoting expression of

232 wn by siRNA resulted in decreased amounts of DNMT1 on chromatin.

233 1, its absence did not impair the loading of DNMT1 onto heterochromatin or the methylation of pericen

234 sfection, reduced DNMT3a expression, but not DNMT1 or -3b, disrupted sarcomere assembly and decreased

235 disrupted two major DNA methyltransferases, Dnmt1 or Dnmt3a, in fetal and adult intestine.

236 ithelium is lethal, while deletion of either Dnmt1 or Dnmt3b has no effect on survival.

237 ic BP overall, associations within strata of DNMT1 or DNMT3B were observed, and the magnitude and the

238 While the deregulated activation of DNMT1 or KIT has been implicated in lung cancer pathogen

239 ro, which were attributed to the hyperactive DNMT1 or KIT, because inactivation of KIT or DNMT1 recip

240 rowth was further supported by the fact that DNMT1 overexpression prevented miR-185-induced inhibitio

241 on; these effects were partially reversed by DNMT1 overexpression.

242 Our results define the RAS/MEK/DNMT1 pathway as a determinant of sensitivity to DNA met

243 Here we show that Dnmt1 preserves DNA methylation through stage I at ICRs

244 ins silencing of IAPs, but in the absence of DNMT1, prolonged binding of NP95 to hemimethylated DNA t

245 transcriptional activators to the endogenous Dnmt1 promoter resulted in robust upregulation of this h

246 In addition, knockdown of DNMT3A or DNMT1 protected neurons against mutant Htt-induced toxic

247 response to oncogenic Ras by downregulating Dnmt1 protein expression leading to inhibition of Dnmt1-

248 ng TCR signal inactivates GSK3beta to rescue DNMT1 protein from proteasomal degradation, and strong T

249 mic or overexpression decreased the level of DNMT1 protein in HCC cells.

250 This dose decreased DNMT1 protein in peripheral blood mononuclear cells by >

251 A permanent reduction of DNMT1 protein using short hairpin RNA (shRNA)-mediated D

252 Moreover, we show that mutant DNMT1 proteins translocate to the cytoplasm and are pron

253 DNMT1 or KIT, because inactivation of KIT or DNMT1 reciprocally blocked decitabine(R) or PKC412(R) ce

254 p disrupts its binding to the ICR of H19 and Dnmt1 recruitment.

255 y, we generated a doxycycline-responsive tTA-DNMT1* rescue line and readily obtained homozygous DNMT1

256 Our results suggest mutations in DNMT1 result in imbalanced protein homeostasis through a

257 n contrast to findings in mouse, deletion of DNMT1 resulted in rapid cell death in human ESCs.

258 plex that includes the DNA methyltransferase DNMT1, resulting in DNA hypermethylation and transcripti

259 ively, these results delineate the nature of DNMT1-RNA interactions and suggest strategies for gene-s

260 ein using short hairpin RNA (shRNA)-mediated DNMT1 silencing rendered HCC cells insensitive both to c

261 striking switch to Pu.1 interaction with the Dnmt1, Sin3A, Nurd, CoRest, and B-Wich corepressor famil

262 rivatives as potent inhibitors of DNMT3A and DNMT1, some showing certain isoform selectivity.

263 of 14-3-3 in NIH3T3 cells led to decrease in DNMT1 specific activity resulting in hypomethylation of

264 Here, we determined that DNMT1-specific inhibition in arterial wall ameliorates t

265 sequence domain led to a strong reduction of DNMT1 stimulation.

266 Transient DNMT1 suppression revealed gDMD-like sequences requiring

267 s the mechanistic understanding of the UHRF1/DNMT1 tandem and the development of assays for the ident

268 lation studies, we identify ISL1 as a direct DNMT1 target, hypermethylated and downregulated in mamma

269 ampal expression of DNA methyltransferase 1 (Dnmt1) that maintains the imprinting of Dio3 and Igf2 du

270 ora kinase B, the centromeric cohesin ESCO2, DNMT1, the ubiquitin-ligase (DZIP3) and the histone meth

271 the previously determined structure of mouse DNMT1, this study also reveals a number of distinct stru

272 F1 increases the activity and specificity of DNMT1, thus exerting a multifaceted influence on the mai

273 HRF1, and thus the probability of recruiting DNMT1 to faithfully duplicate the DNA methylation profil

274 ing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is es

275 vation, TCR and TGF-beta signals converge on DNMT1 to modulate the expression of foxp3 epigenetically

276 UHRF1 has been shown to recruit DNMT1 to replicated DNA by the ability of its SET and RI

277 nding RING E3 ubiquitin ligase that recruits DNMT1 to sites of newly replicated DNA through ubiquityl

278 ndings show that apart from the targeting of DNMT1 to the replicated DNA UHRF1 increases the activity

279 ontrols the UHRF1-mediated ubiquitination of DNMT1 to timely regulate its abundance during S and G2 p

280 ilitates binding of DNA methyltransferase 1 (Dnmt1) to DNA substrates, including the ICRs of the impr

281 s, but a reduction of KIT expression ablates DNMT1 transcription by STAT3 pathway leading to in-paral

282 NR1), DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transient receptor potential vanilloid type 1 (T

283 finger protein 20-like 1 (PHF20L1) regulates DNMT1 turnover in mammalian cells.

284 inase-3beta, which resulted in inhibition of DNMT1 ubiquitination and proteosomal degradation.

285 mediated acetylation of Dnmt1 and subsequent Dnmt1 ubiquitylation and degradation.

286 an target of rapamycin (mTOR) suppressed the DNMT1 up-regulation both in vitro and in vivo.

287 SCD was safe in this study and, by targeting DNMT1, upregulated HbF in RBCs.

288 All 5-CSRTT deficits were associated with DNMT1 upregulation, whereas impulsive behavior could be

289 a 30-fold preference for inhibition of human DNMT1 versus human or murine DNMT3A or -3B, inhibited gl

290 In cultured endothelial cells, DNMT1 was enhanced by oscillatory shear stress (OS), and

291 ary expression of the DNA methylation enzyme DNMT1 was stable in CR mice but increased over time in o

292 we inhibited miR-342 in MCF7 cells and found DNMT1 was up-regulated with no change in EVL expression,

293 blation of the maintenance methyltransferase Dnmt1, we demonstrate that reducing DNA methylation caus

294 A demethylation induced by acute deletion of Dnmt1, we saw an increase in sense transcription at TEs,

295 Five novel mutations of DNMT1 were discovered; p.C353F, p.T481P, p.P491L, p.Y524

296 Surprisingly, we find that DNMT1, which is generally a transcriptional repressor, i

297 ere we report the crystal structure of human DNMT1 with all the structural domains (hDNMT1, residues

298 MicroRNAs (miRNAs) bind directly to DNMT1 with high affinity.

299 sibly owing to a compensating interaction of DNMT1 with PCNA.

300 o identify minimal doses active in depleting DNMT1 without cytotoxicity.