ライフサイエンスコーパス: DTT

1 DTT activity was more strongly associated with emergency

2 DTT also restored most of the activity that was lost upo

3 DTT enhancement of the NMDAR response was dependent on C

4 DTT is feasible in term newborns and may help to charact

5 DTT likely functions in a ligand substitution reaction t

6 DTT prevents oxidation of BH4 in both isoforms, but in n

7 DTT was performed to segment bilateral pyramidal tracts

8 DTT, in contrast, induces a conversion to the extended f

9 stitution reaction that generates a [2Fe-2S]-DTT species, which can transfer the cluster to either la

10 amine synthetase from inactivation by Fe(3+)/DTT, showing that it is an active peroxiredoxin.

11 e PAM-ABP/siRNA polyplex was released by 5mM DTT and heparin.

12 e and collect the sandwich structures, and a DTT solution at elevated temperature is used to release

13 IPK1 E82C/S142C exhibited a DTT-sensitive 5-fold increase in kcat for 3,4,5,6-inosit

14 OxyR and its target genes katG and oxyS in a DTT-reversible manner.

15 xposed to thapsigargin, A23187, brefeldin A, DTT, geldanamycin, or bortezomib manifested reduced acti

16 bonds to reduction by extracellularly added DTT and Triton X-114 detergent phase partitioning.

17 ) treatment; nine Cs could be modified after DTT treatment.

18 atment, and eight Cs could be modified after DTT treatment.

19 l eight Cs could be modified before or after DTT treatment.

20 ponding to OA-NO2; however, a reducing agent DTT (50 mm) or La(3+) (50 mum) completely abolished OA-N

21 Reducing agent DTT and reactive oxygen species (ROS) scavenger tempol,

22 plication of GSH or the thiol-reducing agent DTT can rescue the root phenotype of miao, demonstrating

23 tely blocked by addition of a reducing agent DTT or N-acetyl-L-cysteine, showing that process of oxid

24 Importantly, the sulfhydryl reducing agent DTT was capable of fully restoring complex I activity im

25 ersed in the presence of the reducing agent, DTT, thus suggesting that S-nitrosylation of thiolate-zi

26 Using NEM for thiol alkylation, DTT for disulfide reduction, and mBBr for labeling the r

27 om an urban site were analyzed, and although DTT oxidation was significantly correlated with H2O2 gen

28 rB2) and MsrB3 (hMsrB3) showed that although DTT can function in vitro as the reducing agent, Trx wor

29 ential of two buffer additives (Tween 20 and DTT) to improve the solubility of proteins from shrimp s

30 lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline 1-alkyl-2-acetyl-sn-glycerol

31 the dependence of looping on salt, ATP, and DTT using full-length E2 and an E2 protein containing on

32 anisms for Pho reduction via PhoR(C303A) and DTT are different.

33 oreover, using enzymatic deglycosylation and DTT derivatization combined with mass spectrometry techn

34 -Ras-GppNHp/NOR1A complex, and PEG, DTE, and DTT stabilize the anticatalytic conformation observed in

35 The SDS- and DTT-resistant oligomerization was studied further as it

36 TCEP is 35-fold more reactive than TCEP, and DTT is essentially unreactive.

37 were able to complement the temperature and DTT sensitive phenotype, although a truncated eroA, miss

38 ycin, brefeldin A (brefA), thapsigargin, and DTT that lead to accumulation of unfolded proteins withi

39 ds, including l-homocysteine thiolactone and DTT, induced VEGF expression 7.9- and 8.8-fold.

40 tically favorable buffer containing zinc and DTT reduced the interchain disulfide bond releasing and

41 separate environmental conditions (zinc and DTT treatment) yielded transcription networks consistent

42 s were incubated with cell stressors such as DTT, IFN, and adherent-invasive E coli or control agents

43 rement is based on the dithiothreitol assay (DTT assay), uses colorimetric detection, and can be comp

44 ant enzymes, six Cs could be modified before DTT treatment, and eight Cs could be modified after DTT

45 = 0.91), no correlation was observed between DTT oxidation and (*)OH formation.

46 Our results show that measuring both DTT consumption and ROS generation in the DTT assay is i

47 Partial rescue of activity for aged 20S by DTT implies oxidation of functionally significant cystei

48 LA(2)beta inactivation that is attenuated by DTT or ATP.

49 P, and inhibition of autophosphorylation by DTT.

50 and brefA, whereas acute ER stress caused by DTT and thapsigargin leads to rapid and specific degrada

51 mum rate constant for persulfide cleavage by DTT to k(cat) suggests that persulfide cleavage is, in f

52 diminished under mild reducing conditions by DTT and enhanced by H(2)O(2) oxidation.

53 tate and was blocked rather than enhanced by DTT.

54 tion and remained sensitive to inhibition by DTT, suggesting that the mechanisms for Pho reduction vi

55 over, H(2)O(2) reversed ADAM17 inhibition by DTT.

56 esponse to acid stimulation was mitigated by DTT, suggesting that it arises from sulfhydryl oxidation

57 hydrolysis by reduced glutathione but not by DTT.

58 e-out patches, this crosslink was reduced by DTT and reoxidized by a membrane-impermeant bis-quaterna

59 in an oxidized form and was not reducible by DTT.

60 bonds and measure their rate of reduction by DTT.

61 ed this activity, which could be reversed by DTT.

62 for UA, alternative antioxidants (vitamin C, DTT, and N-acetylcysteine) also enhanced the chemotactic

63 DEP was highly redox-active, causing DTT to decay at a rate of 23-61 pmol min(-1) mug(-1) of

64 ion under three different stress conditions (DTT, H2O2, and nitrogen starvation) using the GFP-tagged

65 because oxidant-treated iPLA(2)beta contains DTT-reducible oligomers, and oligomerization occurs with

66 Dithiothreitol (DTT) is the standard reagent for reducing disulfide bond

67 Dithiothreitol (DTT) was used in all solutions from the beginning, consi

68 A dithiothreitol (DTT) assay was used to measure the ROS-generation potent

69 Adding dithiothreitol (DTT) is the standard method for liquefaction prior to pr

70 versed by the reducing agent dithiothreitol (DTT) and the specific deglutathionylation reagent glutar

71 ted using the reducing agent dithiothreitol (DTT) in an assay that allowed the time for deoxygenation

72 nversely, the reducing agent dithiothreitol (DTT) selectively enhanced NMDAR response to a greater ex

73 locked by the reducing agent dithiothreitol (DTT).

74 tion with the reducing agent dithiothreitol (DTT, 1 mM) prevented drug-induced inhibition of channel

75 by the thiol-reducing agent dithiothreitol (DTT, 10 mm) and inhibited by the oxidizing agent diamide

76 The thiol reducing agent dithiothreitol (DTT, 5.0 mm) both prevented and reversed HNO action, con

77 cts of three reducing agents-dithiothreitol (DTT), beta-mercaptoethanol (beta-MCE), and tris(2-carbox

78 river of HOOH production and dithiothreitol (DTT) loss in ambient PM extracts.

79 e relies on formaldehyde and dithiothreitol (DTT), but these active chemicals may introduce artifacts

80 esence of suspended NP's and dithiothreitol (DTT).

81 A semi-automated dithiothreitol (DTT) analytical system was used to measure daily average

82 Cs could be modified before dithiothreitol (DTT) treatment; nine Cs could be modified after DTT trea

83 equires the presence of both dithiothreitol (DTT) and 4 equiv of Fe(II) for maximum activity.

84 potential (OP) determined by dithiothreitol (DTT) consumption and intracellular reactive oxygen and n

85 ing environment generated by dithiothreitol (DTT) in vivo inhibited Pho induction in a PhoR-dependent

86 nlabeled Fdx is catalyzed by dithiothreitol (DTT), a result that was confirmed by mass spectrometry.

87 cs of disulfide reduction by dithiothreitol (DTT).

88 can be partially reversed by dithiothreitol (DTT).

89 erivatives using a cell-free dithiothreitol (DTT) assay under simulated physiological conditions (37

90 ulin disulfide reductions in dithiothreitol (DTT) over a range of heater temperatures (22-70 degrees

91 lecule of the MerB inhibitor dithiothreitol (DTT).

92 reas in the presence of 4 mm dithiothreitol (DTT) we found no significant differences in the stabilit

93 h postcolumn introduction of dithiothreitol (DTT) and ammonium hydroxide, each disulfide-containing p

94 stable isotopic variants of dithiothreitol (DTT) and MS analysis.

95 The relative efficacy of dithiothreitol (DTT) and tris(2-carboxyethyl)phosphine (TCEP) for reduci

96 The sulfhydryls of dithiothreitol (DTT) compete with thiophosphates for binding to the merc

97 ogical pH in the presence of dithiothreitol (DTT), and shows typical half-times of equilibration in t

98 n assayed in the presence of dithiothreitol (DTT), the inhibitory effect was drastically reduced.

99 n the presence or absence of dithiothreitol (DTT).

100 er dithioerythritol (DTE) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence o

101 rom the presence of residual dithiothreitol (DTT), a reagent that reduces cell viability and interfer

102 ation of the model substrate dithiothreitol (DTT).

103 measurement is based on the dithiothreitol (DTT) assay, where, after being oxidized by PM, the remai

104 ter (PM) was measured by the dithiothreitol (DTT) assay.

105 rticulate matter (PM) in the dithiothreitol (DTT) assay.

106 articles was measured by the dithiothreitol (DTT) assay.

107 ngine was examined using the dithiothreitol (DTT) assay.

108 insignificant impact on the dithiothreitol (DTT) oxidase activity of ALR.

109 imers that were sensitive to dithiothreitol (DTT), dependent on the Mip domain and on at least one cy

110 hat these are susceptible to dithiothreitol (DTT).

111 free drug was verified using dithiothreitol (DTT) and glutathione (GSH) as liberating agents.

112 hemical derivatization using dithiothreitol (DTT) of the phospho-serine/threonine-containing peptides

113 ntrated reducing agent, viz. dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP), was added

114 when cells were treated with dithiothreitol (DTT) but not Mn(2+).

115 stability when treated with dithiothreitol (DTT) compared with monothiol analogues.

116 f purified portal rings with dithiothreitol (DTT) resulted in the disruption of the rings, suggesting

117 Treatment of sera with dithiothreitol (DTT) showed that the majority of remaining lymphocytotox

118 iked sample was treated with dithiothreitol (DTT) to convert disulfide-bonded glutathione to GSH.

119 ld nanoparticle surface with dithiothreitol (DTT), which simplifies the assay and increases its quant

120 do esters, when treated with dithiothreitol (DTT)/diisopropylethylamine (DIPEA), undergo both azido g

121 Pretreatment of IPAs with dithiothreitol (DTT, 1 mm), proposed to promote the conversion of mitoch

122 NaCl, 0.5% NP-40, and 10 mM dithiothreitol [DTT].

123 D was found to be induced by dithiothrietol (DTT), through the MisR/S regulatory system.

124 mall molecules and proteins faster than does DTT.

125 across endosomal membranes by the T-domain (DTT) in response to acidification.

126 ends of the narrow optical gap copolymer DPP-DTT with PC70BM show two distinct spectrally flat region

127 levels of several inflammatory genes in EDTA/DTT when compared to enzymatically released cells.

128 el (i.e. terminal ileum) biopsies using EDTA/DTT and enzymatic release followed by magnetic bead sort

129 Elimination of this bond, by either DTT-mediated reduction or mutagenesis, enhances gating e

130 hylrhodamine-labeled rPfP2 protein exhibited DTT- and SDS-resistant oligomerization when treated with

131 /E229C)(+), only responded to 5-HT following DTT treatment in both homomeric and heteromeric receptor

132 column, and both fractions were analyzed for DTT activity and water-soluble metals.

133 nce of various reductants and oxidants (GSH, DTT, cysteine, O2, hydrogen peroxide, and superoxide), w

134 imethyl- > dimethyl- > monomethyl- > TCEP >> DTT; tmTCEP is 35-fold more reactive than TCEP, and DTT

135 ed a set of residues that likely define a Hg/DTT binding site.

136 buffering test to determine that the MerB/Hg/DTT complex acts as a substrate for the mercuric reducta

137 n fine structure spectroscopy of the MerB/Hg/DTT complex have shown that the ligands to the mercuric

138 The stability of the MerB/Hg/DTT complex, even in the presence of a large excess of c

139 ng the NMR data of free MerB and the MerB/Hg/DTT complex, we identified a set of residues that likely

140 nsive hydrophobic groove adjacent to this Hg/DTT binding site.

141 To determine if DTT treatment homogenizes the bacterial distribution wit

142 rsions of MerA and a nonphysiological Hg(II)-DTT-MerB complex qualitatively support a pathway for dir

143 Importantly, DTT abrogates NS5A-NS5A interactions but does not affect

144 as evident from a substantial attenuation in DTT response after passing PM extracts through the C-18

145 results showed no significant differences in DTT consumption rate measured by the two methods.

146 Fe is mostly inactive in DTT oxidation, but shows synergistic effect in (*)OH for

147 n HU and a tolerance to mutation at Lys63 in DTT.

148 Cu(II), a dominant metal in DTT oxidation, contributes almost negligibly to the ROS

149 nd their mixtures show different patterns in DTT oxidation versus ROS generation.

150 /MS analyses of tryptic digests and included DTT-reversible events, e.g., formation of disulfide bond

151 icles (LDGV) exhibited the highest intrinsic DTT activity, followed by biomass burning (BURN) and hea

152 donor-labeled (H)FTACs and acceptor-labeled DTT upon addition of lipid vesicles indicates that the p

153 presence of oxygen and a reducing agent like DTT.

154 posing the oocytes to a competing thiol like DTT (dithiothreitol) and 2-ME (2-mercaptoethanol).

155 uble cell fractions in the presence of 10 mM DTT, shows UDP-N-acetylglucosamine 6-dehydrogenase activ

156 gnetic resonance) and is based on monitoring DTT-dependent (13)C chemical shift changes of the human

157 interaction of surfactants with a model MP, DTT, using a variety of spectroscopic techniques.

158 The utility of this new DTT-based system is demonstrated by detecting a mock mRN

159 dation of BH4 in both isoforms, but in nNOS, DTT also inhibits oxidation of two key cysteines in nNOS

160 yields active mutant enzymes that exhibit no DTT-irreversible oxidative inactivation.

161 At neutral pH, however, >99% of DTT thiol groups are protonated and thus unreactive.

162 a more careful comparison of the ability of DTT and Trx to function as reducing agents with the vari

163 ependent and irreversible (in the absence of DTT), ultimately resulting in protein denaturation.

164 ces superoxide in the presence or absence of DTT.

165 incubation at 37 degrees C in the absence of DTT.

166 nate (p-XSC) was reversed by the addition of DTT; this suggests the formation of DTT-reducible seleni

167 Burkholderia multivorans between aliquots of DTT-treated sputum samples with and without a mechanical

168 h concentrations along with large amounts of DTT.

169 increases linearly with the concentration of DTT and exponentially with mechanical tension along the

170 s linearly dependent on the concentration of DTT, it is exponentially dependent on the applied force,

171 by adjusting the relative concentrations of DTT and Hb.

172 The linear dependence of DTT's enthalpy of unfolding on the surfactant concentrat

173 Insights into the disposition of DTT on membranes were obtained using a novel hydropathy

174 , presumably because of slow dissociation of DTT-Cu complexes.

175 n, several peptides within the CH2 domain of DTT-IYG showed differential deuterium uptake in the pept

176 st step, we have evaluated the energetics of DTT binding to lipid vesicles using three single-cystein

177 his reversibility permitted free energies of DTT interactions with vesicles to be determined for the

178 ition of DTT; this suggests the formation of DTT-reducible selenium-sulfur bonds between selenocyanat

179 pical BH4 radical in a manner independent of DTT.

180 Site-selective labeling of DTT with fluorescent dyes indicates that the surfactants

181 tential and to estimate historical levels of DTT activity for use in an epidemiologic analysis for th

182 d thioredoxin (Trx), although high levels of DTT can be used as the reductant in vitro.

183 In the presence of DTT and the NO inhibitor N(omega)-nitro-L-arginine methy

184 Unexpectedly, in the presence of DTT, doxorubicinol enhanced the rate of Ca(2+) uptake by

185 The role of DTT is also different.

186 The specificity of DTT chemical derivatization was evaluated separately und

187 surfactants does not alter the structure of DTT.

188 values that are ~1 unit lower than those of DTT and forms a disulfide with a similar E degrees ' val

189 This direct validation of DTT measurements by histological methods in monkeys was

190 nection length in the primate brain based on DTT imaging.

191 Cys651-sulfenic acid formation could be one DTT-reversible inactivation event because Cys651 modific

192 nces in aliquots that were subjected to only DTT treatment and those of the aliquots which included a

193 OP(DTT) was measured for 196 d (mean=0.32 nmol/min/m(3), in

194 Lag 0-2 OP(DTT) remained a significant predictor of asthma and isch

195 Lag 0-2 OP(DTT) was associated with ED visits for multiple cardiore

196 Lag 0-2 OP(DTT) was associated with ED visits for respiratory disea

197 Although OP(DTT) per mass (toxicity) was highest for ultrafine parti

198 Same-day OP(DTT) was not associated with ED visits for any outcome.

199 average (lag 0-2) of OP(DTT) or same-day OP(DTT).

200 1.05 per interquartile range increase in OP(DTT)), asthma (RR=1.12, 95% CI: 1.03, 1.22), and ischemi

201 the same, except that for water-insoluble OP(DTT) the compounds were absorbed on surfaces of soot and

202 In contrast, water-insoluble OP(DTT) was bimodal, with both fine and coarse modes.

203 mes, providing support for the utility of OP(DTT) as a measure of fine particle toxicity.

204 Contrasts in the phases of these forms of OP(DTT) deposited in the respiratory system may have differ

205 ither the 3-d moving average (lag 0-2) of OP(DTT) or same-day OP(DTT).

206 un to estimate the health associations of OP(DTT) while controlling for other pollutants.

207 tem was used to measure daily average OP (OP(DTT)) in water-soluble fine PM at a central monitor site

208 ions of water-insoluble and water-soluble OP(DTT) (dithiothreitol assay, measure of oxidative potenti

209 Water-soluble OP(DTT) was unimodal, peaked near 1-2.5 mum due to contribu

210 methylglyoxal and NADH, NADPH, F 420H 2, or DTT to a M. jannaschii cell extract stimulated the produ

211 d with an anticatalytic conformation (DTE or DTT).

212 aining chloride salts and no formaldehyde or DTT.

213 ch were disrupted by the addition of 2-ME or DTT, which reduced the single disulfide bond found in Pr

214 (thioredoxin/thioredoxin reductase/NADPH or DTT), the enzyme inactivation results from its covalent

215 e coupled in good yields with either TCEP or DTT as the reductant, though some byproducts are observe

216 nd bovine MsrA efficiently use either Trx or DTT as reducing agents.

217 Msr reaction in the absence of either Trx or DTT.

218 ssed ER, treated acutely with tunicamycin or DTT, either is comparable to homeostasis or significantl

219 to ER stress caused by either tunicamycin or DTT.

220 The quinones, known to oxidize DTT in the efficiency order of PQ > 5H-1,4NQ > 1,2-NQ >

221 proteins under three external perturbations (DTT, H(2)O(2) and nitrogen starvation) and two genetic m

222 ial, allowing selective detection of reduced DTT.

223 being oxidized by PM, the remaining reduced DTT is analyzed by the microfluidic sensor.

224 l groups in p65, whereas the thiol reductant DTT reversed the inhibiting effect of H2S on the p65 DNA

225 reduced and reactivated with the reductants DTT and gluthathione, whereas only the catalytic domain

226 ein treated with three different reductants (DTT, beta-mercaptoethanol, and TCEP).

227 The on-line sensor reported DTT consumption rates (oxidative activity) in good corre

228 ry cells from a solution containing residual DTT to a buffer solution.

229 ur acoustofluidic technique removes residual DTT generated in sputum liquefaction and facilitates imm

230 Aglycosylated constructs N297D/S298T (DTT)-K326I/A327Y/L328G (IYG) and N297D/S298A-IYG optimal

231 after dissolving in dithiothreitol solution (DTT 0.8M).

232 lipid bilayers rapidly (>30-fold faster than DTT), whereas the parent TCEP is impermeant.

233 ligible decay, and TCEP was more stable than DTT.

234 CaMKII activity assays established that DTT increased CaMKII activity in CA1 cytosolic extracts

235 ified between injury groups, suggesting that DTT may provide more sensitive measures.

236 The DTT activation could be indicative of the presence of a

237 The DTT activities of water and methanol extracts were corre

238 The DTT assay response was significantly higher for the meth

239 The DTT-mediated growth in the NMDAR response was not observ

240 The DTT-phosphopeptides were enriched by covalent disulfide-

241 The DTT-treated fungal cells also showed evidence for the in

242 dox activity on their own as measured by the DTT assay, but they enhanced ROS generation catalyzed by

243 71 and C151 cysteine thiols, produced by the DTT-dependent reduction of their disulfide, are two addi

244 with one hydrophobic residue eliminated the DTT effect but with two hydrophobic residues made the pe

245 dy, we found that Cys(8) is required for the DTT-sensitive mono- and diubiquitination of Pex20.

246 the complex consist of both sulfurs from the DTT molecule and one cysteine ligand, C96, from the prot

247 ine the relationship between activity in the DTT assay and toxicology measurements across particles o

248 th DTT consumption and ROS generation in the DTT assay is important to incorporate the synergistic co

249 s continued assessment of the utility of the DTT activity assay as a measure of ROS-generating potent

250 The association of the DTT activity of water-soluble PM2.5 (WS_DTT) with these

251 ase with the depth of the sigma holes of the DTT anionophores.

252 isulfides in onconase are less accessible to DTT(red).

253 Exposure of cells to DTT immediately before CO exposure also dramatically red

254 ide and enzymatic O-deglycosylation prior to DTT reaction.

255 C, whether in 5 or 50mM MgCl(2), relative to DTT and the absence of reductant.

256 ctiveness of all four phosphines relative to DTT has been determined using model disulfides, includin

257 vA is slightly down-regulated in response to DTT stress yet up-regulated in response to expression of

258 or ER-associated degradation in response to DTT-mediated ER stress.

259 These properties were sensitive to DTT, suggesting that avicins affect one or more critical

260 RN contributed the largest fraction to total DTT activity over the study period, followed by LDGV and

261 does not increase the turnover number toward DTT.

262 3 Tesla scanner for diffusion tract tracing (DTT) reconstruction of callosal bundles from different a

263 nance imaging diffusion tensor tractography (DTT).

264 assay was validated against the traditional DTT assay using 13 extracted aerosol samples including u

265 s validated off-line against the traditional DTT assay using filter samples taken from urban environm

266 ith the latter transformation dependent upon DTT and an intact Fe-S center.

267 Global profiling of gene expression upon DTT treatment revealed a network of AP2 transcription fa

268 d if lysates were pretreated with 8 M urea + DTT.

269 ere treated with denaturants (SDS, 8 M urea, DTT, or trypsin) before BNP.

270 Using DTT(red) as the reducing agent, the kinetics of the redu

271 ccomplished in a reducing environment, using DTT as an external stimulus, and the thiol constituents

272 carried out at different temperatures using DTT(ox) as the oxidizing agent, and the results were com

273 original bio-barcode assay method, utilizing DTT, has streamlined and simplified probe preparation an

274 ung metastases further confirmed the variant DTT-IYG to be the best at restoring wild-type-like prope

275 d one [4Fe-4S](2+) cluster per homodimer via DTT-induced two-electron reductive coupling of two [2Fe-

276 r electron acceptor for ALR than oxygen when DTT is the reducing substrate.

277 ected for all mutants, even at pH 7 at which DTT is believed to be in a fully folded membrane-incompe

278 With DTT, a new approximately 75 G wide radical EPR was obser

279 Treatment of 25 with DTT in neutral buffer at room temperature demonstrated t

280 0% of the activity with Trx as compared with DTT, raising the possibility that, in animal cells, Trx

281 substances, HULIS), was also correlated with DTT activity in both the water (R = 0.78) and methanol e

282 2S](2+) cluster by anaerobic incubation with DTT and Fe(2+) ion in the absence of exogenous sulfide.

283 e, we report a novel technique, Instant with DTT, EDT, And Low temperature (IDEAL)-labeling, for rapi

284 s A and C, TBE-31 may directly interact with DTT and protein targets such as Keap1 that contain react

285 es that the surfactants do not interact with DTT uniformly, instead concentrating in the most hydroph

286 nstitutive HAC1 splicing that interacts with DTT-mediated perturbation of protein folding.

287 the spectrum of tract lengths obtained with DTT closely matches that estimated from histological rec

288 tion appeared better in samples reduced with DTT in MeOH.

289 For in-gel rehydration, samples reduced with DTT were diluted with sample buffer containing 2-hydroxy

290 y could not be reactivated when reduced with DTT.

291 Reduction with DTT significantly potentiated GABA-induced currents in a

292 reductase activity that was reversible with DTT treatment, whereas graded cross-link lengthening gra

293 lished when the Wnt peptide was treated with DTT, and did not occur with a linear (non-disulfide-bond

294 al alpha-azido ester group, was treated with DTT/DIPEA.

295 enzyme can be reactivated by treatment with DTT and Fe(II).

296 ame antibody accessible after treatment with DTT, suggesting that the N termini are linked by interch

297 ral semiquinone during aerobic turnover with DTT.

298 Without DTT, EPR showed a mixture of superoxide and biopterin ra

299 The digests were treated with or without DTT and analyzed by SDS-PAGE and Western blotting.

300 t only when the digests were treated without DTT.