ライフサイエンスコーパス: Dabcyl

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1 th a fluorophore (fluorescein) and quencher (dabcyl).

2 contain a donor/acceptor FRET pair (FITC and DABCYL).

3 ents a superior alternative to the much-used dabcyl.

4 In comparison with the organic quencher Dabcyl (4,4'-dimethylaminophenyl azo benzoic acid), meta

5 peptide flanked by the FRET fluorophore pair DABCYL and EDANS.

6 nd 4-(4-dimethylaminophenylazo)benzoic acid (DABCYL) are attached to the N-terminus and the lysyl sid

7 (4-(4-dimethylaminophe nyl azo)benzoic acid (DABCYL))-Arg and (7-methoxycoumarin-4-yl)acetyl (MCA))-S

8 4-(4'-dimethylaminophenylazo) benzoic acid (DABCYL) as a quencher.

9 und to be superior to the corresponding Cy3B/DABCYL Beacon.

10 substrate DNA modified with the fluorescein/dabcyl couple allowed to measure AA concentration within

11 1-Pyreneacetamide, 1-pyrenecarboxamide, and DABCYL derivatives have been used in preparation of mole

12 ophenylazo)benzoyl and 5-carboxyfluorescein (Dabcyl/Fam) pair and are based on known cleavage sequenc

13 e nucleic acid was modified with fluorescein/dabcyl FRET pair across the cleavage site.

14 dimethylamino)phenyl)azo) benzoic acid (FITC/DABCYL) FRET pair to produce a signal upon substrate cle

15 ing tested than with more traditional (EDANS/DABCYL) FRET protease substrates.

16 Ala-Arg peptide bond separates the quencher, DABCYL, from the donor, EDANS, and results in a large in

17 l-permeable FRET substrate SM-P124 (Ac-GRRRK(Dabcyl)-GGAANC(MeOBn)RMGG-fluorescein).

18 te stimulation, depends on the presence of a DABCYL group on the peptide N terminus.

19 The CD23-based substrate, Dabcyl-His-Gly-Asp-Gln-Met-Ala-Gln-Lys-Ser-Lys(Fam)-NH2,

20 The advantage of hydrodabcyl over dabcyl is exemplarily demonstrated for the cleavage of t

21 Therefore, fluorescein-Dabcyl-labeled Foerster resonance energy transfer (FRET)

22 TPs as the IAEDANS comes into contact with a Dabcyl-labeled, one-base gapped DNA.

23 The precursor TNF-alpha-based substrate, Dabcyl-Leu-Ala-Gln-Ala-Homophe-Arg-Ser-Lys(Fam)-NH2, is

24 annealed to a complementary 18-nucleotide 5'-Dabcyl-modified DNA.

25 e selected beacons was located adjacent to a DABCYL moiety in the capture oligonucleotide; addition o

26 rene monomers on the 5' end and the quencher DABCYL on the 3' end, were engineered and employed for r

27 A fluorescent donor (Edans) and quencher (Dabcyl) pair was added to the C- and N-termini of a supp

28 poration of a bulky gamma-phosphate-O-linker-dabcyl substituent.

29 ic acid (IAEDANS) and the DNA substrate with Dabcyl, we are able to observe rapid fingers closing in

30 A very popular dark fluorescence quencher is dabcyl, which is a hydrophobic azobenzene derivative.

31 onfirmed that the best substrate, FRET-P2 [K(Dabcyl)YRVAFQKLAE(Edans)K], was approximately 100-fold m

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