ライフサイエンスコーパス: Dicer

1 zed mechanism by which hypoxia downregulates Dicer.

2 e mosquito Aedes aegypti by interfering with Dicer.

3 r precursors by the ribonucleases Drosha and Dicer.

4 manner independent of its interactions with dicer.

5 -stranded RNA substrates by the endonuclease Dicer.

6 icate somewhat more slowly in the absence of Dicer.

7 interference pathway by inhibiting the RNase Dicer.

8 cer deletion retained at least one allele of Dicer.

9 y Ca(2+)-dependent proteolytic activation of Dicer.

10 dent on host cell Argonaute 2 (AGO2) but not Dicer.

11 mphoma to develop with biallelic deletion of Dicer.

12 ery of helicase-dependent functions in other Dicers.

13 two additional microRNA pathway components, dicer-1 and argonaute1, indicating that they are not due

14 to the miRNA effector AGO1 was inhibited by Dicer-1 and its partner Loqs.

15 gest that metamorphosis impairment caused by Dicer-1 and miRNA depletion is due to a deregulation of

16 sely, Loqs-PB alone interacted with mosquito dicer-1 and was essential for full miRNA production.

17 in activator of PKR (PACT), while Drosophila Dicer-1 associates with Loquacious (Loqs).

18 In the present work, we have observed that Dicer-1 depletion results in an increase of mRNA levels

19 se deep sequencing to show that depletion of dicer-1 increases the diversity of small RNAs in AGO1, i

20 s, and that depletion of Kr-h1 expression in Dicer-1 knockdown individuals rescues metamorphosis.

21 In Drosophila, Dicer-1 produces approximately 22-24-nt miRNAs from pre-

22 In 2009 we reported that depletion of Dicer-1, the enzyme that catalyzes the final step of miR

23 hich impaired metamorphosis, and by treating Dicer-1-depleted individuals with an miR-2 mimic to allo

24 restricting pre-miRNA processing in flies to Dicer-1.

25 SC loading complex (RLC), which contains the Dicer-2 (Dcr-2)-R2D2 complex and recruits duplex siRNA t

26 erved that the helicase domain of Drosophila Dicer-2 (dmDcr-2) governs substrate recognition and clea

27 microscopy to solve structures of Drosophila Dicer-2 alone and in complex with blunt dsRNA.

28 ncreasing expression of either Su(var)3-9 or Dicer-2 also led to an increase in life span.

29 qs-PD enhances the rate of dsRNA cleavage by Dicer-2 and also enables processing of substrates normal

30 -independent, but we find that modulation of Dicer-2 cleavage also requires dsRNA binding by Loqs-PD.

31 sly showed that inorganic phosphate inhibits Dicer-2 cleavage of pre-miRNAs, but not long dsRNAs.

32 , two pathways are proposed, either based on Dicer-2 cleavage to generate 20-nucleotide vsRNAs or bas

33 double-stranded RNA (dsRNA), and Drosophila Dicer-2 distinguishes dsRNA substrates by their termini.

34 ding protein that functions as a cofactor of Dicer-2 in Drosophila.

35 Mutation of Dicer-2 led to an increase in DNA double-strand breaks.

36 ely 22-24-nt miRNAs from pre-miRNAs, whereas Dicer-2 makes 21-nt siRNAs from long double-stranded RNA

37 well as increased life span in TE-sensitized Dicer-2 mutants.

38 bstrate by a phosphate-binding pocket in the Dicer-2 PAZ (Piwi, Argonaute, and Zwille/Pinhead) domain

39 hored by the phosphate-binding pocket in the Dicer-2 PAZ domain and the distance between the pocket a

40 a pair of conserved arginine residues in the Dicer-2 PAZ domain blocked cleavage of short, but not lo

41 tions in the phosphate-binding pocket of the Dicer-2 PAZ domain decreased RNA silencing activity in v

42 How Dicer-2 precisely makes 21-nt siRNAs with a remarkably h

43 Further, we show that Dicer-2 predominantly targets viral dsRNA and produces 2

44 ds light on the molecular mechanism by which Dicer-2 produces 21-nt siRNAs with a remarkably high fid

45 sphate-dependent substrate discrimination by Dicer-2 reflects dsRNA substrate length.

46 directly interact with the Hel2 subdomain of Dicer-2's helicase domain.

47 ding overexpression of Sir2, Su(var)3-9, and Dicer-2, as well as decreased expression of Adar, mitiga

48 nt, to a considerable degree overlap between Dicer-2-related (19 to 21 nt) and Dicer-2-unrelated vsRN

49 ap between Dicer-2-related (19 to 21 nt) and Dicer-2-unrelated vsRNAs, suggesting a common origin for

50 cruit substrates into the helicase domain of Dicer-2.

51 Deleting miRNAs by Dicer ablation (Dicer KO) in thymocytes selectively impa

52 Phosphorylated Dicer (p-Dicer) accumulates in the nucleus and is recruited to DN

53 DNA damage-induced nuclear Dicer accumulation is conserved in mammals.

54 DICER activity assays confirmed impaired DICER activity

55 In line with this observation, enhancing DICER activity by a small molecule, enoxacin, is benefic

56 DICER activity assays confirmed impaired DICER activity following cigarette smoke exposure.

57 Complete ablation of Dicer activity in a Pten null mouse model for prostate c

58 Dicer activity in crude cell lysates is increased in the

59 Disrupting Dicer activity leads to an increase in apoptosis and sen

60 ndent functions of mutant p53 in controlling Dicer activity.

61 ut of the microRNA (miRNA)-processing enzyme Dicer (ADicerKO), as well as humans with lipodystrophy,

62 f double-stranded RNA and the recruitment of DICER, AGO1, AGO2 and the G9a histone lysine methyltrans

63 Pre-miRNAs, along with Dicer, AGO2, and TRBP, are present in exosomes of cancer

64 sion of this mutant Dicer, but not wild-type Dicer, also resulted in a partial inhibition of Influenz

65 Loss of Dicer, an RNase III-like enzyme critical in microRNA bio

66 but not NiggA led to decreased expression of Dicer and Ago 2, suggesting that NiggV interaction with

67 ess granule formation and re-organization of DICER and AGO2 protein interactions with their partners.

68 ouse model with conditional deletion of both Dicer and Bim, to determine the biologic significance of

69 ssociate with the aberrant miRNA profiles in Dicer and Drosha cKO spermatozoa.

70 Germline-specific Dicer and Drosha conditional knockout (cKO) mice produce

71 d here, in which the ASncmtRNAs are bound to Dicer and knockdown of the ASncmtRNAs reduces reporter l

72 st evidence establishing the significance of dicer and microRNA in promoting endothelial cell tumor g

73 B to repress its expression independently of Dicer and microRNAs.

74 neurodegeneration and further suggests that DICER and miRNAs affect neuronal integrity and are possi

75 nfected cells is mediated by wild-type human Dicer and potently suppressed by both NS1 of IAV as well

76 ted double-knockout human cells lacking both Dicer and protein kinase RNA-activated.

77 ted helicase 3 (DRH-3) is an ortholog of the Dicer and RIG-I family of double-strand RNA activated AT

78 However, both Dicer and the Argonaute protein family have expanded rol

79 ion of essential miRNA biogenesis components Dicer and TRBP is increased following latent KSHV infect

80 enes, including the well-characterized gene, dicer, and a probable uncharacterized cyclin dependent k

81 tent with reduced microRNA processing enzyme Dicer, and increased expression of Alu element in OIR.

82 Here, we report that Dicer- and Drosha-dependent diRNAs function as guiding m

83 B core RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding protei

84 Here, we review the evidence for Dicer as an innate antiviral across species.

85 calpain inhibitor prevented loss of platelet dicer as well as the diabetes mellitus-induced decrease

86 An in vitro Dicer assay shows that this rG4 inhibits Dicer processin

87 These results demonstrate the role of Dicer-associated RNA binding proteins in maintenance of

88 Human Dicer associates with HIV TAR RNA-binding protein (TRBP)

89 to contribute to this process, their mode of Dicer binding and their genome-wide effects on miRNA pro

90 sRBPs are reported to homodimerize, with the Dicer-binding region implicated in self-association.

91 rs in the presence of endogenously expressed Dicer but is no longer detectable when the Dicer cDNA is

92 Remarkably, overexpression of this mutant Dicer, but not wild-type Dicer, also resulted in a parti

93 processing through the partial depletion of Dicer, can promote enhanced metastatic potential.

94 cin (mTOR) signaling pathway was enhanced in Dicer(-/-) CD4(+) T cells, and its pharmacological inhib

95 ficantly enhanced the induction of iTregs in Dicer(-/-) CD4(+) T cells.

96 interfering RNAs enhanced Treg induction in Dicer(-/-) CD4(+) T cells.

97 d Dicer but is no longer detectable when the Dicer cDNA is overexpressed.

98 The most highly upregulated gene in the Dicer-CKOMG MG is the proteoglycan Brevican (Bcan) and o

99 n, we used a conditional deletion of Dicer1 (Dicer-CKOMG) in retinal Muller glia (MG).

100 s, initiates from short introns but bypasses Dicer cleavage.

101 DICER cleaves the stem-loop structure from pre-miRNAs, a

102 Dicer controls the biogenesis of microRNAs (miRNAs) and

103 tive 5p miRNAs into Argonaute proteins via a Dicer-coupled 5' monophosphate-dependent strand selectio

104 d suppressors of G0 defects in cells lacking Dicer (dcr1Delta), which mapped to genes involved in chr

105 y assembled genomes, RNaseIII (Drosha and/or Dicer) deficient samples and single cells (at both embry

106 We find that Dicer-deficient CD4 T cells fail to correctly discrimina

107 Excess IL-2 production by Dicer-deficient CD4 T cells was sufficient to override a

108 Drosha and Dicer-deficient cells, devoid of most miRNAs, are hypers

109 specifically upregulated in Drosha- but not Dicer-deficient ES cells.

110 RBP or PACT was designed and introduced into Dicer-deficient mammalian cells, revealing selective def

111 Dicer-deficient MHCI-restricted alphabeta T cells fail t

112 ecifying transcription factor Runx3, whereas Dicer-deficient MHCII-restricted alphabeta T cells show

113 significance of increased Bim expression in Dicer-deficient nephron progenitors.

114 Use of Dicer-deficient or Rab27a and Rab27b double-deficient Tr

115 did not confer protection from apoptosis, as Dicer deletion in established p53-null B-cell lymphomas

116 a clones analyzed that survived Cre-mediated Dicer deletion retained at least one allele of Dicer.

117 ver, a deficiency in p53 neither rescued the Dicer deletion-induced delay in Myc-driven B-cell lympho

118 ells, as they underwent rapid apoptosis upon Dicer deletion.

119 o a prolonged G1/S transition via decreasing DICER-dependent biogenesis of miRNA let-7, which increas

120 Here we show that Dicer-dependent loss of microRNAs in these neurons of ad

121 rigenic epithelial cells to form tumors in a Dicer-dependent manner.

122 region gene 8 (Dgcr8)/Drosha-independent but Dicer-dependent manner.

123 Our studies reveal that DICER-dependent microRNAs are essential for gonadotropin

124 Thus, DICER-dependent microRNAs confer a new layer of transcri

125 Loss of DICER-dependent microRNAs in gonadotropes resulted in pr

126 We found that many of the DICER-dependent microRNAs, predicted in silico to bind g

127 is unique to nematodes, the second involves Dicer-dependent RNA-directed DNA methylation, hitherto u

128 although HSV-1, which encodes at least eight Dicer-dependent viral miRNAs, did replicate somewhat mor

129 We show here that Dicer depletion can promote the invasive behavior of cel

130 Dicer depletion causes endogenous DNA damage and delays

131 miR-630 is a promising approach to overcome Dicer deregulation in cancer.

132 ific inactivation of key miRNA pathway genes Dicer, Dgcr8, and the entire Argonaute gene family (Ago1

133 Although loss of Dicer did not compromise the cellular response to virus

134 rm-PAZ domains have been considered the only Dicer domains that bind dsRNA termini, unexpectedly, we

135 However, we found that knockdown of DICER dramatically increased cell resistance to camptoth

136 nstrate that ERK phosphorylates and inhibits Dicer during meiosis I for oogenesis to proceed normally

137 ory effect of HBx protein on activity of the Dicer endoribonuclease.

138 icase functions, we investigated two related Dicer enzymes from the thermophilic fungus Sporotrichum

139 Dicer enzymes function at the core of RNA silencing to d

140 tinct activities in the context of different Dicer enzymes.

141 actor antibody in mice resulted in rescue of Dicer expression and significantly decreased tumor growt

142 we identify tumour hypoxia as a regulator of DICER expression in large cohorts of breast cancer patie

143 We show that DICER expression is suppressed by hypoxia through an epi

144 However, how dicer expression levels and miRNA biogenesis are regulat

145 that during zebrafish hindbrain development dicer expression levels are controlled by miR-107 to tun

146 Surprisingly, we found that knocking down Dicer expression suppresses the growth and tumorigenic c

147 d tumor growth and metastasis, and decreased Dicer expression.

148 ypoxic conditions, targets and downregulates Dicer expression.

149 ously demonstrated that the endoribonuclease DICER facilitates chromatin decondensation during lesion

150 r eukaryotes, the microRNA biogenesis enzyme Dicer forms a 1:1 association with a dsRNA-binding prote

151 ial endonucleolytic processing by Drosha and Dicer from longer RNA polymerase II (RNAP II)-transcribe

152 on of the microRNA (miRNA)-processing enzyme Dicer from nephron progenitors results in premature depl

153 calcemia and uremia is dependent upon intact dicer function and miRNAs.

154 phorylation of Dicer on either site inhibits Dicer function in the female germline and dampens small

155 us recombination repair (HRR) via decreasing DICER-generated small RNAs at the damage sites.

156 o produce pre-microRNA in the nucleus, while DICER generates not only mature microRNA, but also endog

157 Recent findings suggest that noncanonical Dicer generates small noncoding RNA to mediate the DNA d

158 PT-Dicer(-/-) glands cultured in low-calcium medium secrete

159 ng pathway, and in adipose tissue, levels of dicer have been shown to decrease with age, increase wit

160 To examine the evolutionary origins of Dicer helicase functions, we investigated two related Di

161 pecific loss of the miRNA-processing enzyme, Dicer, identified intestinal epithelial cells (IEC) and

162 TRBP and PACT show that the proteins bind to Dicer in a similar manner and by mutual exclusion.

163 By inactivating Dicer in adult quiescent hepatocytes we avoided the hepa

164 hypoxia in the downregulation of Drosha and Dicer in cancer cells that leads to dysregulation of miR

165 Additionally, KO of dicer in cultured brown preadipocytes promoted a white a

166 nadotropin beta subunits in vivo, we deleted Dicer in gonadotropes by a Cre-lox genetic approach.

167 Furthermore, mutants lacking Dicer in gonadotropes displayed severely reduced fertili

168 ctive depletion of the miR-processing enzyme Dicer in mature myeloid cells blocks angiogenesis and me

169 NA endonuclease, which functions upstream of Dicer in microRNA biogenesis, also regulates Cd4 and Cd8

170 These results uncover a novel function of DICER in regulating the cell cycle through miRNA biogene

171 ges facilitated by Drosha in the nucleus and Dicer in the cytoplasm.

172 In comparison, hemizygous loss of Dicer in the same model also reduces primary tumor burde

173 these results highlight a pleotropic role of Dicer in tumorigenesis that is not only dosage-dependent

174 al deficiency of the miRNA-processing enzyme Dicer in vitro and in vivo.

175 NA (miRNA) biogenesis, vertebrate mir-451 is Dicer independent.

176 The eIF1A-Ago2 assembly facilitates Dicer-independent biogenesis of miR-451, which mediates

177 R-10a maturation by binding pre-miR-10a in a DICER-independent manner.

178 iously that antisense transcription triggers Dicer-independent siRNA (disiRNA) production and disiRNA

179 Thus, repression via dicer-independent siRNA-mediated facultative heterochrom

180 s PTH secretion, demonstrating that they are dicer-independent.

181 iRNA binding unit connected by a linker to a Dicer inhibiting unit.

182 our studies define a previously unrecognized Dicer interaction interface and suggest that Loqs-PD is

183 The endoribonuclease Dicer is a key component of the human RNA interference p

184 The endoribonuclease dicer is a major component of the miRNA-processing pathw

185 Dicer is a multifunctional protein that is essential acr

186 Dicer is a specialized nuclease that produces RNA molecu

187 Dicer is an RNase III enzyme essential for the maturatio

188 that the post-transcriptional regulation of Dicer is controlled by the cell density-mediated localiz

189 Although Dicer is essential for general microRNA (miRNA) biogenes

190 We also demonstrated that Dicer is involved in vsRNA1 generation in infected cells

191 Here, we show that human Dicer is phosphorylated in the platform-Piwi/Argonaute/Z

192 shown that hypoxia downregulates Drosha and Dicer, key enzymes in miRNA biogenesis, causing a decrea

193 link to rRNA was almost completely lost in a DICER knock-out cell line.

194 This response to dicer knockdown was mediated by up-regulated miR 21a-3p

195 of the lymphomas that emerged in conditional Dicer knockout Emu-myc transgenic mice.

196 discovering true miRNA motifs in three mouse Dicer-knockout experiments from different tissues, two o

197 ferentiation, was found up-regulated in iNKT Dicer KO cells together with enhanced TGF-beta signaling

198 effector differentiation, displayed by iNKT Dicer KO cells.

199 were differentially expressed between WT and Dicer KO iNKT cells at different differentiation stages

200 Deleting miRNAs by Dicer ablation (Dicer KO) in thymocytes selectively impairs iNKT cell su

201 Accordingly, miR-200s are downregulated in Dicer-KO CDs, its direct target genes Zeb1, Zeb2, and Sn

202 ression, we show that a mutant form of human Dicer lacking the amino-terminal helicase domain can pro

203 Loss of miR-107 function stabilizes dicer levels and miR-9 biogenesis across the ventricular

204 We propose that miR-107 modulation of dicer levels in differentiating neuronal cells is requir

205 Dicer levels were altered in platelets from diabetic mic

206 YAP, leading to Let-7-dependent reduction in Dicer levels.

207 mediated RdDM requires PolV and DRM2 but not Dicer like-3 and other PolIV pathway components.

208 s an antiviral defense through the action of DICER-like (DCL) and ARGONAUTE (AGO) proteins.

209 ncing mechanism relying on the generation by Dicer-like (DCL) enzymes of virus-derived small RNAs of

210 These pathways rely on distinct Dicer-like (DCL) proteins that process double-stranded R

211 We identified homologs of DICER-LIKE (DCL), ARGONAUTE (AGO), and other genes invol

212 c RNA-directed RNA polymerase (RdR1) and the Dicer-like (DCL3 and DCL4) proteins are recruited during

213 A MINIMAL DICER-LIKE (mDCL) gene, which lacks the N-terminal helic

214 Arabidopsis thaliana, tRFs are processed by Dicer-like 1 and incorporated into Argonaute1 (AGO1), ak

215 tion in a mutant lacking 22- to 24-nt sRNAs (dicer-like 2, 3, 4 triple mutant).

216 ere, we demonstrate that DRB3 functions with Dicer-like 3 (DCL3) and Argonaute 4 (AGO4) in methylatio

217 to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonau

218 ), RNA-dependent RNA polymerase 2 (RDR2) and DICER-like 3 (DCL3).

219 utants from this screen, there were three at Dicer-like 3 that failed to produce both miRNAs and siRN

220 with the most C-terminal dsRBM domain of the Dicer-like 4 (DCL4) proteins.

221 is was shown previously to contribute to the DICER-LIKE 4 (DCL4)-mediated biogenesis of viral small i

222 ss of Paramecium sRNAs, produced by a unique Dicer-like enzyme, that likely provides late stage quali

223 ubsequently processed into small RNAs by the DICER-LIKE enzymes.

224 in an analysis of data derived from multiple Dicer-like mutants in Arabidopsis.

225 tors are mostly produced by Botrytis cinerea Dicer-like protein 1 (Bc-DCL1) and Bc-DCL2.

226 Moreover, disrupting Dicer-like protein 4 or RDR6, the biogenesis genes for t

227 of vsiRNA sequences reflect different plant Dicer-like proteins and Argonautes involved in vsiRNA bi

228 ate an antagonistic relationship between the Dicer-like proteins at at least some methylation loci.

229 ci in response to loss of one or more of the Dicer-like proteins that indicate an antagonistic relati

230 A processing machinery (i.e., independent of DICER-LIKE proteins).

231 -5s and tRF-3s is not primarily dependent on DICER-LIKE proteins, though they seem to be associated w

232 t family of RNA-acting prim-pol domains with DICER-like proteins.

233 RASE IV, RNA-DEPENDENT RNA POLYMERASE-2, and DICER-LIKE-4.

234 the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).

235 os mutant for a strong hypomorphic allele of DICER-LIKE1 (dcl1-15).

236 discovered that it contains a mutation in a dicer-like1 homolog, a key enzyme required for microRNA

237 sion-dependent RdDM, which functions through DICER-LIKE3 (DCL3) but bypasses the requirement of both

238 hese loci depends upon P. patens homologs of DICER-LIKE3 (DCL3), RNA-DEPENDENT RNA POLYMERASE2, and t

239 Plant DICER-LIKE4 (DCL4) performs dual functions, acting in an

240 d primarily transcriptionally >100-fold upon Dicer loss and is resistant to resilencing by complete r

241 strands and with properties consistent with Dicer-mediated cleavage of the dsRNA genome.

242 Here we show that Roquin enhances Dicer-mediated processing of pre-miR-146a.

243 mation of a pre-miR-aptamiR complex inhibits Dicer-mediated processing of the pre-miR, which is requi

244 Dicer-mediated processing of virus-specific dsRNA into s

245 Here, we report that DICER mediates the recruitment of the methyltransferase

246 cemia increased serum PTH >4-fold less in PT-Dicer(-/-) mice compared with control mice with no incre

247 Remarkably, the PT-Dicer(-/-) mice did not increase serum parathyroid hormo

248 Moreover, uremic PT-Dicer(-/-) mice increased serum PTH and FGF23 significan

249 In contrast, the PT-Dicer(-/-) mice responded normally to activation of the

250 ted parathyroid-specific Dicer1 knockout (PT-Dicer(-/-) ) mice where parathyroid miRNA maturation is

251 hy exhibited a substantial downregulation of dicer mRNA expression.

252 well as an ill-defined boundary phenotype in Dicer mutants.

253 miRNA-dependent repression, as confirmed in Dicer-null cells.

254 Phosphorylation of Dicer on either site inhibits Dicer function in the fema

255 We show that ERK phosphorylates Dicer on two conserved residues in its RNase IIIb and do

256 Inhibition of Dicer or Ago2 expression revealed that small RNAs are in

257 In addition, knockdown of DICER or AGO2 using either siRNA or MOE-gapmer chemistri

258 iral activity in the presence and absence of Dicer or Drosha, the RNase III nucleases responsible for

259 Phosphorylated Dicer (p-Dicer) accumulates in the nucleus and is recrui

260 o contributed to reversal of the loss of the dicer phenotype.

261 (2014) report that Ras signaling results in Dicer phosphorylation, which induces its nuclear localiz

262 families of crystal structures of the human Dicer "platform-PAZ-connector helix" cassette in complex

263 ells and B-cell lymphomas to survive without Dicer, presenting a potential therapeutic opportunity fo

264 cted to bind oncogenic pre-miR-155, inhibits Dicer processing under simulated physiological condition

265 tro Dicer assay shows that this rG4 inhibits Dicer processing, supporting the potential role of rG4 s

266 xpression, confirming hPMR1 acts upstream of Dicer processing.

267 The enzyme Dicer produces small silencing RNAs such as micro-RNAs (

268 ases KDM6A/B and results in silencing of the DICER promoter.

269 Recently, it was reported that knockdown of DICER reduced the ATM-dependent DNA damage response and

270 nents of the antiviral RNAi pathway, such as Dicer-related helicase 1 (DRH-1), to display hypersuscep

271 The Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) is an ortholog of the D

272 Dicer relies on the correct measurement of RNA target du

273 ditional phosphorylation of carboxy-terminal Dicer residues (S1728 and S1852).

274 This Dicer-resistant epigenetic switch confers tumorigenicity

275 Together, these Dicer-resistant genes constitute an mRNA expression sign

276 ression of their downstream targets PTEN and Dicer, respectively, suggesting that Rbfox2 indirectly r

277 that the levels and substrate selectivity of DICER result in the preferential biogenesis of specific

278 isense transcription and availability of the Dicer ribonuclease are the key determinants for primary

279 udy, we demonstrate that inactivation of the Dicer RNA endonuclease in murine thymocytes impairs init

280 idues is necessary and sufficient to trigger Dicer's nuclear translocation in worms, mice, and human

281 Tumors that formed in the dicer shRNA group were significantly smaller than tumors

282 ce injected with EOMA cells transfected with dicer shRNA.

283 cule named Targapremir-210 that binds to the Dicer site of the miR-210 hairpin precursor.

284 CD43 mediates the accumulation of Dicer specifically in cancer exosomes.

285 nical pre-miRNAs is not a general feature of Dicer substrate hairpins.

286 Core lipid nanoparticles (LNP) loaded with a Dicer substrate small interfering RNA targeting catenin

287 omprise a substantial fraction of endogenous Dicer substrates in mammalian genomes.

288 s and enters the miRNA biogenesis pathway as Dicer substrates.

289 Rictor was more abundantly expressed in Dicer(-/-) T cells as was mTOR, and their expression was

290 sed on the structure, a catalytically active Dicer that cannot bind TRBP or PACT was designed and int

291 relationship between mutant p53, TAp63, and Dicer that might contribute to the metastatic function o

292 Reduced expression of DICER, the enzyme involved in microRNA processing, is fr

293 ADARs interact with Dicer to augment the processing of pre-miR-27a to mature

294 Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and D

295 ximately 70-nucleotide pre-miRNA hairpins by Dicer to generate mature approximately 22-nucleotide miR

296 s localized processing of nuclear dsRNA by p-Dicer to promote DNA repair.

297 We solved the crystal structure of the human Dicer-TRBP interface, revealing the structural basis of

298 ription and cleavage by the DROSHA/DGCR8 and DICER/TRBP/PACT complexes.

299 It is thought that Dicer uses its multidomain architecture to calibrate RNA

300 T4/7 to prevent final maturation of let-7 by Dicer, whereas LIN28B has been suggested to preferential

301 Collectively, we place Dicer within the context of the DDR by demonstrating a D