ライフサイエンスコーパス: Dispase

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1 thelium (organoids) by using collagenase and dispase.

2 y of PVR correlated with increasing doses of dispase.

3 f trypsin were mimicked by collagenase I and Dispase (0.1%, 1 min each) but not by chymotrypsin, Pron

4 roduced by treating porcine or rat skin with Dispase and Triton X-100.

5 skeletal muscle tissue with collagenase XI, dispase and trypsin followed by plating the resultant mu

6 igestion cocktail containing collagenase and dispase, and it involves separation over discontinuous p

7 An intrinsic substrate of MTG is the dispase autolysis-inducing protein (DAIP).

8 cascade of events was probably triggered by dispase, causing native cells and factors to produce PVR

9 In addition, we identified a pH-sensitive dispase cleavage site in hexon HVR1, which depends on th

10 Dispase cleavage, which we find maps to a specific site

11 othesis was that an intraocular injection of dispase could trigger events that would cause PVR.

12 multimeric plasma VWF, and to a fragment of dispase-digested plasma VWF (residues Leu(480)/Val(481)-

13 neal pure epithelial sheets were isolated by dispase digestion from human tissues and dissociated int

14 Because the proteolytic enzyme dispase dissociates tissues, the hypothesis was that an

15 (2+) leads to a loss of adhesion measured by dispase fragmentation assay (lambda = 15.1 min).

16 prepared using RPE cell sheets isolated with Dispase from Royal College of Surgeons normal (RCS rdy+

17 on cells freshly isolated, using the enzyme dispase, from the mouse fourth ventricle choroid plexus.

18 epithelial medium (SHEM) containing 50 mg/mL dispase II and 100 mM sorbitol.

19 belted rabbits by incubation of 50 mg/mL of dispase II in supplemental hormonal epithelial medium (S

20 al cells were digested with collagenase A or Dispase II in supplemented hormonal epithelial medium (S

21 ting for 18 hours at 4 degrees C in 15 mg/mL dispase II with sorbitol in defined keratinocyte serum-f

22 by a two-step enzymatic dissociation method (dispase II/trypsin-EDTA), and cultured in low to medium

23 d rabbits was injected with 0.003 U to 1.0 U dispase in the subretinal space or vitreous cavity.

24 In the dispase-induced rabbit model of PVR, the ability of the

25 tive vitreoretinopathy (PVR) was tested in a dispase-induced rabbit PVR model.

26 Dispase initiated the development of PVR without the add

27 for a surgical retinal break at the time of dispase injection was also examined.

28 Collagenase, but not dispase, isolated subjacent mesenchymal cells, of which

29 chieved by mixing expanded single cells with dispase-isolated epithelial cells in 3D Matrigel.

30 The dispase model of PVR was technically easy to perform, pe

31 Digestion with collagenase A, but not Dispase, of the stripped Descemet's membrane generated H

32 Human limbus was isolated by dispase or collagenase.

33 re cultured on the basement membrane side of dispase-pretreated AM, with or without seeding rabbit co

34 nsitivities to SDS-mediated dissociation and dispase proteolysis.

35 n addition to validating the newly developed dispase PVR rabbit model, the results indicate that ribo

36 We used dispase-separated epidermis, followed by intercellular d

37 Dispase-Triton-treated allogenic ADM was useful as a der

38 rypsin or a dissolved mixture of collagenase/dispase was inoculated into xenografts derived from the

39 ed in response to subretinal or intravitreal dispase, with or without creation of a controlled retina

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