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1                                              E. chaffeensis binding to and subsequent infection of mo
2                                              E. chaffeensis BolA bound to the promoters of genes enco
3                                              E. chaffeensis bolA complemented a stress-sensitive E. c
4                                              E. chaffeensis CtrA bound to the promoters of late-stage
5                                              E. chaffeensis experiences temperature changes during tr
6                                              E. chaffeensis expresses a sensor kinase, PleC, and a co
7                                              E. chaffeensis has a multigene family of major outer mem
8                                              E. chaffeensis inclusions were labeled with the transfer
9                                              E. chaffeensis induced rapid tyrosine phosphorylation of
10                                              E. chaffeensis infection activated the phosphatidylinosi
11                                              E. chaffeensis infection did not result in dramatic chan
12                                              E. chaffeensis infection in a human monocyte cell line (
13                                              E. chaffeensis infects mononuclear phagocytes and is tra
14                                              E. chaffeensis strains induce strikingly variable inflam
15                                              E. chaffeensis TRP75 and E. canis TRP95 were immunopreci
16                                              E. chaffeensis was sensitive to closantel, an HK inhibit
17                                              E. chaffeensis, therefore, can recruit interacting signa
18                                              E. chaffeensis-specific cytotoxic T cells were not detec
19 responses to E. chaffeensis lipoproteins, 13 E. chaffeensis lipoprotein genes were cloned into a mamm
20                                       All 16 E. chaffeensis IFA-positive sera reacted with rP30.
21  to analyze the antibody responses of the 37 E. chaffeensis indirect fluorescent-antibody assay (IFA)
22                      Nearly the entire (99%) E. chaffeensis P120 gene (1,616 bp), the 14-repeat regio
23 129S6-Cd4(tm1Knw) mice also developed active E. chaffeensis-specific immunoglobulin G responses that
24                                   Additional E. chaffeensis surface proteins detected were OMP85, hyp
25  the white-tailed deer agent, and additional E. chaffeensis-positive samples).
26 ption of the uridine-cytidine kinase affects E. chaffeensis replication in human macrophages.
27                                        After E. chaffeensis infection, canonical and noncanonical Wnt
28    The addition of oxytetracycline 6 h after E. chaffeensis infection caused a decrease in TfR mRNA w
29 f Amblyomma americanum ticks before or after E. chaffeensis transmission to naive dogs.
30 r rise without detectable antibodies against E. chaffeensis.
31 the hypothesis that immune responses against E. chaffeensis would be different if the mice are challe
32            In the absence of MHC-II alleles, E. chaffeensis infections persisted throughout the entir
33                                     Although E. chaffeensis induces the generation of several cytokin
34                                     Although E. chaffeensis lacks entire lipopolysaccharide and most
35 ss spectrometry identified the protein as an E. chaffeensis 12.3-kDa hypothetical protein, which was
36 ted strongly with antibodies in sera from an E. chaffeensis-infected dog and human monocytotropic ehr
37 rophoretic mobility shift assays revealed an E. chaffeensis-encoded protein that specifically bound t
38 ast two-hybrid analysis demonstrated that an E. chaffeensis type 1 secretion system substrate, TRP32,
39       E. ewingii accounted for 20 (91%), and E. chaffeensis accounted for 1 (5%) of the positives.
40                           Some HGE agent and E. chaffeensis inclusions colocalized with major histoco
41 graphic regions where both the HGE agent and E. chaffeensis occur.
42                          Ehrlichia canis and E. chaffeensis are tick-borne obligatory intramonocytic
43  a 28- and a 27-kb locus in the E. canis and E. chaffeensis genomes, respectively.
44        Western blot analysis of E. canis and E. chaffeensis lysates with the anti-rMmpA serum resulte
45 ter membrane proteins (OMPs) of E. canis and E. chaffeensis.
46                            E. canis gp36 and E. chaffeensis gp47 were differentially expressed only o
47  peptide repeat units from E. canis gp36 and E. chaffeensis gp47 were substantially less immunoreacti
48  peptides are conserved between E. muris and E. chaffeensis OMP-19, and they elicited IFN-gamma produ
49 B4, and virB6) of both A. phagocytophila and E. chaffeensis were arranged downstream from a sodB gene
50 ese five genes in both A. phagocytophila and E. chaffeensis were polycistronically transcribed and co
51 d endocytosis directs A. phagocytophilum and E. chaffeensis to an intracellular compartment secluded
52 rkansas genomic library by probing with anti-E. chaffeensis hyperimmune mouse ascitic fluid.
53 n around HGE agent inclusions but not around E. chaffeensis inclusions.
54 his macrophage-tropic bacterium, we assessed E. chaffeensis infections in three mouse strains with di
55     Recently, molecular interactions between E. chaffeensis 47-kDa tandem repeat (TR) protein (TRP47)
56 sferrin receptors (TfRs) accumulated on both E. chaffeensis and E. sennetsu, but not HGE agent, inclu
57 retic mobility shift assays showed that both E. chaffeensis and E. sennetsu infection increased the b
58 which peaked at 24 h postinfection with both E. chaffeensis and E. sennetsu infection in THP-1 or HL-
59                          Infection with both E. chaffeensis and E. sennetsu, but not HGE agent, in th
60            The cytokine-inducing activity by E. chaffeensis PBP provides novel insights into pathogen
61 y indistinguishable from infection caused by E. chaffeensis or the agent of human granulocytic ehrlic
62 protein-processing enzymes were expressed by E. chaffeensis cultured in the human promyelocytic leuke
63 sate, suggesting that it is not expressed by E. chaffeensis cultured in THP-1 cells.
64 r VirB6 proteins and VirB9 were expressed by E. chaffeensis in THP-1 cells, and amounts of these five
65           Multiple lipoproteins expressed by E. chaffeensis in vitro and in vivo may play key roles i
66 e host cell-specific antigenic expression by E. chaffeensis.
67 n of both the IL-8 promoter and NF-kappaB by E. chaffeensis.
68 t canonical and noncanonical Wnt pathways by E. chaffeensis TRP effectors stimulates phagocytosis and
69                         Expression of PBP by E. chaffeensis was upregulated during its intracellular
70 n of host cytokine and chemokine profiles by E. chaffeensis strains underlies the distinct host liver
71 nslocation of bacterially encoded protein by E. chaffeensis and to identify a specific binding motif
72 e the mechanism of TfR mRNA up-regulation by E. chaffeensis and E. sennetsu infection.
73          Only one paralog was transcribed by E. chaffeensis in three developmental stages of Amblyomm
74  Anaplasma phagocytophilum, Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, R. co
75  6 of 52 (11.5%) from Rhode Island contained E. chaffeensis DNA.
76  in mice infected with the tick cell-derived E. chaffeensis compared to DH82-grown bacteria.
77 teins from macrophage- and tick cell-derived E. chaffeensis, respectively.
78  and Rhode Island for PCR analysis to detect E. chaffeensis DNA.
79 CL11, which were strongly upregulated during E. chaffeensis infection and were also upregulated by di
80              Among the 38 patients enrolled, E. chaffeensis was isolated from the blood of 7 (18%) an
81                           E. coli expressing E. chaffeensis SurE exhibited increased resistance to os
82                                       First, E. chaffeensis avoided stimulation of or repressed the t
83 ntibodies reactive to E. chaffeensis and for E. chaffeensis-specific 16S rRNA gene fragments by an in
84 ompared with previously described assays for E. chaffeensis.
85 rates that both PCR and culture of blood for E. chaffeensis have high diagnostic yields.
86 rs of tandem repeats, were characterized for E. chaffeensis from white-tailed deer (Odocoileus virgin
87  permissive and nonpermissive conditions for E. chaffeensis growth.
88  nucleotide salvage pathway is essential for E. chaffeensis replication and that it may be important
89  that D. melanogaster is a suitable host for E. chaffeensis.
90 e obtained for HL-60 cells used as hosts for E. chaffeensis and A. phagocytophilum.
91 otide variation than previously reported for E. chaffeensis from infected humans or ticks.
92 e tested whether cholesterol is required for E. chaffeensis and A. phagocytophilum.
93 ge PCR assay, but not by assays specific for E. chaffeensis or the agent of human granulocytic ehrlic
94 feensis-containing vacuoles and vacuole-free E. chaffeensis.
95 sis-containing vacuoles than in vacuole-free E. chaffeensis.
96  not amplify the 200-bp target amplicon from E. chaffeensis, the human granulocytic ehrlichiosis agen
97 esota or Wisconsin were found not to be from E. chaffeensis or E. ewingii and instead to be caused by
98 produce PCR products with DNA extracted from E. chaffeensis-, E. canis-, or E. phagocytophila-infecte
99 era from E. canis-infected dogs but not from E. chaffeensis-infected patients.
100                          Removal of PBP from E. chaffeensis lysate using penicillin affinity column a
101 e actively transcribed in cell-culture grown E. chaffeensis.
102                             Macrophage-grown E. chaffeensis was cleared in 2 weeks from the peritoneu
103 cores, identity of the primers to homologous E. chaffeensis sequences, and the availability of simila
104 med if the ELISA is positive), we identified E. chaffeensis or a serologically and antigenically simi
105                                           In E. chaffeensis we predicted three pairs of putative two-
106 (MSP-2) in A. marginale and HGE and OMP-1 in E. chaffeensis.
107 the N-terminal TR region (18 amino acids) in E. chaffeensis and the complete TR (24 amino acids) in E
108 own to be surface exposed), were detected in E. chaffeensis cultured in human monocytic leukemia THP-
109             The P43 gene was not detected in E. chaffeensis DNA by Southern blot, and antisera agains
110 e sequence polymorphisms in several genes in E. chaffeensis strains have been reported, global genomi
111 ng the protein expression and interaction in E. chaffeensis.
112  phosphorylation of Stat1, Jak1, and Jak2 in E. chaffeensis-infected THP-1 cells was examined by immu
113 y that documents that insertion mutations in E. chaffeensis that cause attenuated growth confer prote
114                        Targeted mutations in E. chaffeensis were created to disrupt two genes, and al
115 idic C terminus of E. canis TRP95 but not in E. chaffeensis TRP75.
116 ssion of penicillin-binding protein (PBP) in E. chaffeensis was analyzed by reverse-transcription pol
117 cted, which was strikingly more prevalent in E. chaffeensis-containing vacuoles than in vacuole-free
118 variable-length PCR target (VLPT) protein in E. chaffeensis.
119             Major immunoreactive proteins in E. chaffeensis (75-kDa) and E. canis (95-kD) whole-cell
120 age-specific expression of the T4S system in E. chaffeensis.
121 is the first to examine genetic variation in E. chaffeensis from a nonhuman vertebrate host.
122  inhibitor, globomycin, was found to inhibit E. chaffeensis infection and lipoprotein processing in H
123 -butyldimethysilyl)-c-di-GMP (CDGA) inhibits E. chaffeensis internalization into host cells by facili
124 egulate nutrient uptake during intracellular E. chaffeensis development at both temperatures.
125 these five proteins were similar in isolated E. chaffeensis-containing vacuoles and vacuole-free E. c
126            Here we demonstrate that isolated E. chaffeensis outer membranes have porin activities, as
127                                   The 28-kDa E. chaffeensis and 30-kDa E. canis native proteins were
128 eral cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglyca
129                                         More E. chaffeensis-infected monocytes transmigrated than uni
130         The current study identified a novel E. chaffeensis ubiquitin ligase and revealed an importan
131                                  Addition of E. chaffeensis resulted in rapid increases in the level
132                                  Analysis of E. chaffeensis and A. phagocytophilum genome sequences r
133                     Western blot analysis of E. chaffeensis membrane and soluble fractions using anti
134 first and the most comprehensive analysis of E. chaffeensis-expressed proteins.
135 s critical for conferring rapid clearance of E. chaffeensis.
136 h PCR target, is useful for PCR detection of E. chaffeensis and differentiation of isolates.
137 e to that of the nested PCR for detection of E. chaffeensis in infected DH82 cells, experimentally in
138 more sensitive than the PCR for detection of E. chaffeensis regardless of the nature of the specimens
139 Thus, this RT-PCR is useful for detection of E. chaffeensis when a high sensitivity is required.
140      During the intracellular development of E. chaffeensis, both P28 and OMP-1F were expressed mostl
141                                 Diagnosis of E. chaffeensis infection by indirect immunofluorescence
142              A type IV secretion effector of E. chaffeensis blocks mitochondrion-mediated host cell a
143 f the members of the p28 multigene family of E. chaffeensis, sera from two beagle dogs experimentally
144                  The 120-kDa protein gene of E. chaffeensis contains four identical 240-bp tandem rep
145 30-kDa major outer membrane protein genes of E. chaffeensis and E. canis, respectively.
146 ated in a 23kb DNA fragment in the genome of E. chaffeensis.
147 These data suggest that the rMAP2 homolog of E. chaffeensis may have potential as a test antigen for
148                        The identification of E. chaffeensis surface-exposed proteins provides novel i
149          Recently, molecular interactions of E. chaffeensis tandem repeat proteins 47 and 120 (TRP47
150    The P28s were divergent among isolates of E. chaffeensis also.
151 c diversity of the P28 among the isolates of E. chaffeensis suggest that P28s may be involved in immu
152 nced and compared in 12 clinical isolates of E. chaffeensis to determine allele variation.
153 res infected with nine different isolates of E. chaffeensis, blood samples from seven patients with m
154             When the three human isolates of E. chaffeensis, each belonging to a different genogroup,
155 ence of p28 among all five human isolates of E. chaffeensis.
156                   More frequent isolation of E. chaffeensis from patients with infection should furth
157 d in this study suggest that the membrane of E. chaffeensis is very complex, having many expressed pr
158 munofluorescent microscopy in the nucleus of E. chaffeensis-infected host cells and was detected in n
159 f E. canis and the corresponding ortholog of E. chaffeensis (47 kDa) were identified and the proteins
160 p30 of Ehrlichia canis (< or =71.3%), p28 of E. chaffeensis (< or =68.3%), and map1 of Cowdria rumina
161  protein with < or =69.1% identity to P28 of E. chaffeensis, < or =67.3% identity to P30 of E. canis,
162 n or closely related interacting partners of E. chaffeensis TRP32, TRP47, and TRP120 demonstrate a mo
163 iated molecular patterns and pathogenesis of E. chaffeensis infection.
164 d chromatography purified, and native PBP of E. chaffeensis were investigated for their ability to in
165  were observed primarily in the periplasm of E. chaffeensis and E. canis.
166 ts revealed distinct virulence phenotypes of E. chaffeensis strains with defined genome sequences.
167                     Although pretreatment of E. chaffeensis with CDGA did not reduce bacterial bindin
168                   Entry and proliferation of E. chaffeensis in THP-1 cells were significantly blocked
169 sponses to another outer membrane protein of E. chaffeensis (GP120) showed similar temporal and quant
170 l amino acid sequence of a 23-kDa protein of E. chaffeensis.
171  recognizing four outer membrane proteins of E. chaffeensis (Arkansas strain) including the 25-, 26-,
172  34 sera reacted with any native proteins of E. chaffeensis ranging from 44 to 110 kDa, and 30 sera r
173 gs suggest that the 30-kDa-range proteins of E. chaffeensis represent a family of antigenically relat
174       To investigate the surface proteins of E. chaffeensis, membrane-impermeable, cleavable Sulfo-NH
175 otal, membrane, and immunogenic proteomes of E. chaffeensis originating from macrophage and tick cell
176 cluding the two-repeat polypeptide region of E. chaffeensis P120.
177 ci was downregulated prior to the release of E. chaffeensis from host THP-1 cells and was upregulated
178 ral responses and the in vivo replication of E. chaffeensis suggests that D. melanogaster is a suitab
179 negative for antibodies against the rMAP2 of E. chaffeensis by using the ELISA.
180    This is the first demonstration of RNA of E. chaffeensis in infected blood and acquisition-fed mal
181 aling regulates aggregation and sessility of E. chaffeensis within the inclusion through stabilizatio
182 t immunization with the p28 of one strain of E. chaffeensis would confer cross-protection against oth
183 tigenic variants of p28 among the strains of E. chaffeensis and the presence of multiple copies of he
184 o compare the genome sequences of strains of E. chaffeensis and to examine the virulence potentials o
185 ikingly different among the three strains of E. chaffeensis: gamma interferon, CCL5, CXCL1, CXCL2, CX
186          This is the most extensive study of E. chaffeensis VLPT and 120-kDa antigen gene genetic var
187 protease HtrA was detected on the surface of E. chaffeensis, and TRP120 was degraded by treatment of
188 d macrophage activation and the synthesis of E. chaffeensis-specific Th1-type immunoglobulin G respon
189 e E. canis p120 is 30% homologous to that of E. chaffeensis p120.
190               Differential transmigration of E. chaffeensis- and A. phagocytophilum-infected leukocyt
191  in southern New England and transmission of E. chaffeensis may occur there.
192                            Heat treatment of E. chaffeensis or the addition of monodansylcadaverine,
193 sis, and TRP120 was degraded by treatment of E. chaffeensis with recombinant E. chaffeensis HtrA.
194 has been implicated as the primary vector of E. chaffeensis.
195 and the variable-length PCR target (VLPT) of E. chaffeensis.
196 lecule inhibitor had a significant impact on E. chaffeensis replication and recruitment of the TRP120
197 tigated the effects of c-di-GMP signaling on E. chaffeensis infection of the human monocytic cell lin
198 nt is unique because it is thus far the only E. chaffeensis recombinant antigen that has been shown t
199 5.8%) goats were positive by diagnostic PCR; E. chaffeensis was isolated in cell culture from one goa
200  To survive and replicate within phagocytes, E. chaffeensis exploits the host cell by modulating a nu
201 his study, we assessed two clonally purified E. chaffeensis mutants with insertions within the genes
202      Genomic DNA was extracted from purified E. chaffeensis strains Wakulla and Liberty, and comparat
203        Western immunoblotting using purified E. chaffeensis and the HGE agent as antigens suggested t
204                                  Recombinant E. chaffeensis PleD showed diguanylate cyclase activity
205 odies in patients' sera with the recombinant E. chaffeensis 120- and 28-kDa proteins as well as the 1
206  by protein immunoblotting using recombinant E. chaffeensis proteins expressed in Escherichia coli.
207 treatment of E. chaffeensis with recombinant E. chaffeensis HtrA.
208 ell knockouts, when challenged with a second E. chaffeensis infection.
209                                      Second, E. chaffeensis up-regulated NF-kappaB and apoptosis inhi
210                [(32)P]c-di-GMP bound several E. chaffeensis native proteins and two E. chaffeensis re
211 n particular, despite its small genome size, E. chaffeensis has four tandem virB6 paralogs (virB6-1,
212              We previously demonstrated that E. chaffeensis is capable of growing in Drosophila S2 ce
213 isiae) two-hybrid analysis demonstrated that E. chaffeensis-secreted tandem repeat protein 120 (TRP12
214             Recently we have determined that E. chaffeensis tandem repeat proteins (TRPs) are type 1
215     Therefore, we tested the hypothesis that E. chaffeensis can infect adult Drosophila melanogaster.
216                       The data indicate that E. chaffeensis is exposed to the extracellular milieu du
217                    We recently reported that E. chaffeensis grown in tick cells expresses different p
218                    We recently reported that E. chaffeensis utilizes a type 1 secretion (T1S) system
219             These novel findings reveal that E. chaffeensis exploits canonical and noncanonical Wnt p
220           Analyses of human sera showed that E. chaffeensis-infected patients also generated serologi
221                     Our results suggest that E. chaffeensis CtrA plays a role in co-ordinating develo
222                     Our results suggest that E. chaffeensis invasion is regulated by c-di-GMP signali
223                     The results suggest that E. chaffeensis VirB9, the quadruple VirB6 proteins, and
224                                          The E. chaffeensis strain Wakulla induces diffuse hepatitis
225                                          The E. chaffeensis strains could be divided into three genet
226                                          The E. chaffeensis T1S effector TRP120 is conjugated to SUMO
227                                          The E. chaffeensis-specific immunoglobulin G response was co
228                              We assessed the E. chaffeensis clearance from the peritoneum, spleen, an
229 e (1,620 bp), and a 2-repeat region from the E. chaffeensis P120 gene (520 bp) were expressed in Esch
230  the same chromosomal location; however, the E. chaffeensis VLPT gene (594 bp) has tandem repeats tha
231 ovel tandem repeat DNA-binding domain in the E. chaffeensis 120-kDa tandem repeat protein (TRP120) th
232 gene deletion and insertion mutations in the E. chaffeensis genome.
233 ple copies of genes homologous to p28 in the E. chaffeensis genome.
234 ed proteins provides novel insights into the E. chaffeensis surface and lays the foundation for ratio
235                                     Like the E. chaffeensis p120, the E. canis p120 contains tandem r
236 gned based on the N-terminal sequence of the E. chaffeensis 28-kDa protein and the consensus sequence
237 ve genes and that antigenic variation of the E. chaffeensis 28-kDa proteins may result from different
238                     In all, 278 genes of the E. chaffeensis genome were verified as functional genes.
239                 Bioinfomatic analysis of the E. chaffeensis genome, however, predicted genes encoding
240 rly one-fourth of all predicted genes of the E. chaffeensis genome, validating that they are function
241 ential in preventing lysosomal fusion of the E. chaffeensis inclusion compartment.
242 el treatment induced lysosomal fusion of the E. chaffeensis inclusion in a human monocytic leukemia c
243 array of 147,027 chromosome positions of the E. chaffeensis strain Arkansas genome.
244             Carbohydrate was detected on the E. chaffeensis and E. canis recombinant proteins, includ
245           Both protective Abs recognized the E. chaffeensis major outer membrane protein (OMP)-1g.
246        In this study, we determined that the E. chaffeensis effector TRP120 is posttranslationally mo
247              These results indicate that the E. chaffeensis Wakulla strain can induce inflammatory re
248 st one serum sample that also reacted to the E. chaffeensis antigen.
249 ween TRP32 and host targets localized to the E. chaffeensis morulae or in the host cell cytoplasm adj
250                                     When the E. chaffeensis strains were inoculated into severe combi
251 tly different between mice infected with the E. chaffeensis originating from tick cells or macrophage
252                                       Third, E. chaffeensis also inhibited the gene transcription of
253                                        Thus, E. chaffeensis is present in ticks in southern New Engla
254 owed a very high prevalence of antibodies to E. chaffeensis (97 of 112; 87%) and a low prevalence of
255 thought to have cross-reacting antibodies to E. chaffeensis.
256 s developed diagnostic titers of antibody to E. chaffeensis.
257 probable human monocytic ehrlichiosis due to E. chaffeensis also had antibodies to the HGE agent in a
258 that residents of Connecticut are exposed to E. chaffeensis, A. americanum ticks were collected in Co
259 uman leukemia cell line THP-1 was exposed to E. chaffeensis, significant upregulation of IL-8, IL-1be
260  seroreactive to Bartonella henselae, one to E. chaffeensis, and one to R. rickettsii antigen; howeve
261  38 (73.7%) goats had antibodies reactive to E. chaffeensis (>/=1:128), and 6 of 38 (15.8%) goats wer
262 icity were tested for antibodies reactive to E. chaffeensis and for E. chaffeensis-specific 16S rRNA
263 hia muris strain that are closely related to E. chaffeensis in C57BL/6 mice.
264 5, an ehrlichial organism closely related to E. chaffeensis isolated from Ixodes ovatus ticks in Japa
265 rlichia muris, a pathogen closely related to E. chaffeensis, resulted in anemia, thrombocytopenia, an
266  and T-cell function on murine resistance to E. chaffeensis.
267 s, are critical for conferring resistance to E. chaffeensis.
268 These data suggest that the host response to E. chaffeensis depends on the source of the bacteria and
269 lecular basis of the host immune response to E. chaffeensis.
270 tein expression and host immune responses to E. chaffeensis lipoproteins, 13 E. chaffeensis lipoprote
271 veral E. chaffeensis native proteins and two E. chaffeensis recombinant I-site proteins, and this bin
272 Ech_0379 mutant and challenge with wild-type E. chaffeensis 1 month following inoculation with the mu
273 ere tested by immunofluorescence (IFA) using E. chaffeensis antigen and by protein immunoblotting usi
274 ay for immunoglobulin M (IgM) and IgG, using E. chaffeensis antigen, identified 44 and 33% of the iso
275                                       Viable E. chaffeensis organisms blocked tyrosine phosphorylatio
276       The heat-sensitive component of viable E. chaffeensis cells was essential for these signaling e
277                             To study in vivo E. chaffeensis lipoprotein expression and host immune re
278 t could mediate bacterial clearance in vivo, E. chaffeensis-specific mAbs were generated and administ
279                                         When E. chaffeensis matures into an infectious form, morulae
280  genes suggest a possible mechanism by which E. chaffeensis might evade the host immune defenses.
281 h lysosomes or Golgi-derived vesicles, while E. chaffeensis resides in an early endosomal compartment
282 oxyl-terminal amino acid homology (59%) with E. chaffeensis VLPT and the same chromosomal location; h
283  strongly with TRP120 in HeLa cells and with E. chaffeensis dense-cored morulae and areas adjacent to
284 morular fibrillar structures associated with E. chaffeensis.
285 ; 1 patient had evidence of coinfection with E. chaffeensis and a spotted fever group rickettsia.
286 t inclusions but was weakly colocalized with E. chaffeensis inclusions.
287 a pattern different from that of humans with E. chaffeensis infection but similar to that of a dog ex
288 two beagle dogs experimentally infected with E. chaffeensis were evaluated for the presence of specif
289  naive dogs, or when dogs were infected with E. chaffeensis, the animals developed delayed-type hyper
290 cteria and that of macrophages infected with E. chaffeensis, we have identified few genes that are co
291 y are naturally exposed to and infected with E. chaffeensis.
292 man peripheral blood monocytes infected with E. chaffeensis.
293 ce of immunocompetent mice to infection with E. chaffeensis and demonstrate the utility of immunocomp
294 tely 25-fold within 30 min of infection with E. chaffeensis.
295  three sera that were IFA positive only with E. chaffeensis, and three sera that were IFA positive on
296 st recombinant P43 (rP43) did not react with E. chaffeensis as detected by indirect fluorescent antib
297 cence contained antibodies that reacted with E. chaffeensis and E. canis antigens in a pattern differ
298 gainst the recombinant proteins reacted with E. chaffeensis P120 and E. canis P140, respectively.
299 nstrated to contain antibodies reactive with E. chaffeensis by indirect immunofluorescence assays (IF
300                     Antibodies reactive with E. chaffeensis were detected in 14 (67%) of the 21 PCR-p

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