ライフサイエンスコーパス: E. coli O157

1 E. coli O157 and non-O157 STEC were detected in 35 and 1

2 E. coli O157 can be detected by culture with sorbitol-Ma

3 E. coli O157 causes a wide range of clinical illness tha

4 E. coli O157 isolates clearly segregated into SNP lineag

5 E. coli O157:H7 can cause bloody diarrhea, hemolytic ure

6 E. coli O157:H7 is an enterohemorrhagic bacteria respons

7 E. coli O157:H7 strains possess two lpf loci encoding hi

8 E. coli O157:H7 survives and persists well in diverse en

9 E. coli O157:H7, suspended in 0.1% peptone, was inoculat

10 Low abundant (<100 cells mL(-1)) E. coli O157:H7 cells were isolated and enriched from en

11 using in vitro-generated populations for 10 E. coli O157:H7 strains.

12 a coli O157:H7 from cultures containing 1000 E. coli O157:H7 colony-forming units (cfu)/mL, or approx

13 de polymorphism (SNP) assay to segregate 148 E. coli O157 isolates from Australia, Argentina, and the

14 ia coli strains, CGH microarray data from 19 E. coli O157 pathogenic test strains were used to demons

15 omic analysis of a comprehensive panel of 25 E. coli O157:H7 strains associated with three nearly sim

16 forming units (cfu)/mL, or approximately 500 E. coli O157:H7 and approximately 500 E. coli K12 cfu/mL

17 tects SNPs in 96 loci and applied it to >500 E. coli O157 clinical strains.

18 Stx binding and uptake within the gut after E. coli O157:H7 infection should result in greater disea

19 cts to protect host epithelial cells against E. coli O157:H7 colonization, at least in part, by promo

20 This work demonstrates that not all E. coli O157:H7 strains belong to the same clonal group,

21 1,25(OH)2D3 altered E. coli O157:H7-induced reductions in transepithelial el

22 Although E. coli O157:H7 Sakai contains more than 1,600 genes not

23 Among E. coli O157 isolates, 57 (88%) were identified by both

24 Differences in HUS frequency among E. coli O157:H7 outbreaks have been noted, but our under

25 An E. coli O157:H7 strain with a stcE gene deletion was not

26 This study characterized an E. coli O157:H7 strain, designated PA2, that belongs to

27 al gut flora can influence the outcome of an E. coli O157 infection in mice.

28 limit for both glucose (0.08+/-0.02muM) and E. coli O157:H7 ( approximately 4 CFUmL(-1)) were compet

29 athogens (Listeria monocytogenesis 19115 and E. coli O157:H7), clinical isolates (methicillin-resista

30 Escherichia coli, Enterococcus faecalis, and E. coli O157:H7 were compared in fresh (river) water and

31 on and detection of Escherichia coli K12 and E. coli O157 targets.

32 teric human pathogens such as Salmonella and E. coli O157:H7.

33 moeba histolytica, Cryptosporidium spp., and E. coli O157:H7; 95% for Giardia lamblia; 94% for ETEC a

34 e B cell upon cellular membrane anchors anti-E. coli O157:H7 IgM.

35 Antibody (human IgG, anti-E. coli O157:H7, and anti-Salmonella) complexes on the s

36 E. coli O157:H7 have been tested using anti-E. coli O157-magnetic beads conjugate (MBs-pECAb) as a c

37 The films functionalized with anti-E. coli O157:H7 and anti-Salmonella antibodies were used

38 anocomposite platform was modified with anti-E. coli O157:H7 monoclonal antibody.

39 nic E. coli K-12 did not colonize as well as E. coli O157:H7 at the bovine terminal rectal mucosa.

40 is region showed that all spinach-associated E. coli O157:H7 isolates harbored this same G:C-->A:T su

41 ates, derived from a 2006 spinach-associated E. coli O157:H7 outbreak, were analyzed, all (194/194) d

42 king the inflammatory response to attenuated E. coli O157:H7 adhesion, mucin 2 (MUC2) expression was

43 s (SNPs) were attributable to six "atypical" E. coli O157 strains and included recombinant regions.

44 we performed competition experiments between E. coli O157:H7 and an isogenic DeltalpfA1 DeltalpfA2 do

45 ribution of flhC in the relationship between E. coli O157:H7 and cattle, we constructed a similar flh

46 gher resolution of the relationships between E. coli O157 isolates than that provided by MLVA.

47 ed in liquid cultures using a bioluminescent E. coli-O157:H7 strain.

48 e major differences between human and bovine E. coli O157 isolates were due to the relative abundance

49 at support vector machine analysis of bovine E. coli O157 isolate sequences can be applied to predict

50 nly a minor subset (less than 10%) of bovine E. coli O157 isolates analyzed in our datasets were pred

51 epithelial cells is mediated by intimin, but E. coli O157:H7 also possess several other putative adhe

52 was increased by TNF-alpha treatment and by E. coli O157:H7 infection.

53 cultivar-specific effects on colonization by E. coli O157, we used 12 different cultivars of lettuce

54 associated with salad leaves contaminated by E. coli O157.

55 resistance by consuming nutrients needed by E. coli O157:H7 to colonize, thus preventing this first

56 imit the availability of sugars preferred by E. coli O157:H7 and perhaps other pathogens.

57 ntrations of endotoxins and bacterial cells (E. coli O157:H19).

58 jority of cattle excrete less than 10(2) CFU E. coli O157/g feces, most studies, including those usin

59 significantly increased survival of E. coli, E. coli O157:H7 and Ent. faecalis in the water column.

60 Whole genome sequencing was used to compare E. coli O157 isolates from host reservoirs (cattle and s

61 contrast, lacks EIIC(Gam), and consequently, E. coli O157:H7 strains cannot utilize Gam.

62 Here, we constructed E. coli O157:H7 gal mutants which presumably have little

63 The sample containing E. coli O157:H7 was circulated through the system in the

64 In contrast, E. coli O157:H7 strains that were missing Stx or hemolys

65 While the amounts of culturable E. coli O157:H7 rapidly decline after introduction onto

66 benzoicus, B. subtilis, Streptomyces sp. D7, E. coli O157:H7, K. pneumoniae, and S. typhimurium were

67 oarray format was used to capture and detect E. coli O157:H7.

68 ay be effectively used to capture and detect E. coli O157:H7.

69 ed biosensor was able to specifically detect E. coli O157:H7 at the low concentration within 10 min i

70 dvances are exploited to successfully detect E. coli O157 in a 500-fold higher background of E. coli

71 ti-Salmonella antibodies were used to detect E. coli O157:H7 and S. enteriditis through label-free IR

72 demonstrating the assay's ability to detect E. coli O157:H7 in the presence of high levels of backgr

73 ess as low as 20 nm was capable of detecting E. coli O157:H7 and Salmonella sp. in complex hamburger

74 of only 1 Da) was sufficient to distinguish E. coli O157:H7 from a non-O157:H7, nonpathogenic E. col

75 x biomarkers, it was possible to distinguish E. coli O157:H7 from a nonpathogenic E. coli by top-down

76 Antibiotic use during E. coli O157:H7 infections is associated with a higher r

77 etter risk in response to novel and emerging E. coli O157:H7 resistance and virulence phenotypes.

78 ducing Escherichia coli (STEC), encompassing E. coli O157 and non-O157 STEC, is a significant cause o

79 coli strains, including the enterohemorragic E. coli O157:H7.

80 E. coli CFT073 and UTI89, enterohemorrhagic E. coli O157:H7, and enterotoxigenic E. coli O78:H11, co

81 precursor to the virulent enterohemorrhagic E. coli O157:H7.

82 st closely associated with enterohemorrhagic E. coli O157:H7-mediated hemorrhagic colitis that someti

83 orrhagic Escherichia coli (EHEC), especially E. coli O157:H7, is an emerging cause of food-borne illn

84 Fewer E. coli O157:H7 cells survived when applied onto plants

85 One hundred five E. coli O157 strains isolated over a 5-year period from

86 To protect consumers from deadly foodborne E. coli O157:H7 infection, it is vital to develop a simp

87 all pectic substrates were anti-adhesive for E. coli O157:H7 binding to human HT29 cells.

88 ting-flow QCM system, capture antibodies for E. coli O157:H7 were first immobilized onto the QCM chip

89 ble smartphone based fluorescence device for E. coli O157:H7 detection.

90 A major risk factor for E. coli O157:H7 infection is exposure to shallow groundw

91 tery, a competent laboratory should look for E. coli O157:H7 and Shiga toxin directly in stool.

92 this new system was as low as 2.3 CFU/mL for E. coli O157:H7 and 5 ng/mL for hepatitis B surface Ag (

93 n, and milk), the LOD was under 5 CFU/mL for E. coli O157:H7, S. typhimurium and L. monocytogenes.

94 fecal pats from 91 farms tested positive for E. coli O157.

95 elationship with a case testing positive for E. coli O157:H7 and coincident diarrheal illness.

96 All samples testing positive for E. coli O157:H7 contained deer feces, and 5 tested farm

97 15% of the heifers were culture positive for E. coli O157:H7, while 15 to 22.5% of the animals were c

98 mental samples from farm A were positive for E. coli O157:H7.

99 which of these options is most relevant for E. coli O157:H7 on leafy green produce, we developed and

100 Cattle are the main reservoir for E. coli O157, and vaccines for cattle now exist.

101 Positive selection for E. coli O157:H7 across the farms identified only one pos

102 ed fresh strawberries as a novel vehicle for E. coli O157:H7 infection, implicated deer feces as the

103 ingesting contaminated food, most frequently E. coli O157:H7 in ground beef or fresh produce.

104 e PCR (qPCR) was used to test for genes from E. coli O157:H7 (eaeO157), shiga-toxin producing E. coli

105 Finally, a homologue of lymphostatin from E. coli O157:H7 (ToxB; L7095) was also found to possess

106 A specific DNA sequence from E. coli O157:H7 having 22 mers as an amine-terminated pr

107 e food contaminant Escherichia coli O157:H7 (E. coli O157:H7) in complex food products due to the rec

108 y could prevent substantial numbers of human E. coli O157 cases.

109 ant for the clinical manifestations of human E. coli O157:H7 infection for their contribution to the

110 nly adaptation and genome diversification in E. coli O157:H7 but also contributing to the development

111 f OtpA into the extracellular environment in E. coli O157:H7 requires OtpB and that when OtpA was pro

112 We inactivated gadE in E. coli O157:H7 Sakai and compared global transcription

113 ce of infection, and therefore, reduction in E. coli O157:H7 prevalence in cattle by vaccination repr

114 ormation is complex and poorly understood in E. coli O157:H7.

115 r the formation of GlcNAc-P-P-Und by WecA in E. coli O157.

116 rotect the gut from colonization by incoming E. coli O157:H7.

117 itol 3-kinase (PI3K)/Akt signaling increased E. coli O157:H7 adherence to HT-29 cells.

118 at cultivar-specific root exudate influences E. coli O157 activity.

119 but was better, and not affected by initial E. coli O157 numbers, above this concentration.

120 their target organisms were used to isolate E. coli O157:H7 and S. typhimurium separately from a coc

121 Enterococcus faecalis, Escherichia coli K12, E. coli O157:H7, Salmonella enterica serovar Typhimurium

122 Finally, utilizing QDs to label E. coli O157:H7 in cell mixtures results in greater accu

123 and rapid method which can detect low level E. coli O157:H7 in foods at real-time.

124 . coli vs. S. enteriditis) and strain level (E. coli O157:H7 vs E. coli K12).

125 sp. D7, Bacillus subtilis, B. licheniformis, E. coli O157:H7, Klebsiella pneumoniae, Enterobacter clo

126 of cattle showed that E. coli ONT:H25, like E. coli O157:H7, colonized the bovine recto-anal junctio

127 Shigella flexneri and as a factor mediating E. coli O157:H7 adherence.

128 g samples with 10(3), 10(4) and 10(5) CFU/mL E. coli O157:H7 were 106.98, 96.52 and 102.65 (in yogurt

129 complement complex, was protective in murine E. coli O157:H7 infection.

130 ferent from other Stx-positive and -negative E. coli O157:H7 strains and were more similar to MG1655

131 30%), culturable amounts of the nontoxigenic E. coli O157:H7 strain ATCC 700728 and the virulent stra

132 ied by enzymes in the E. coli serotype O157 (E. coli O157) O-PS biosynthetic pathway.

133 Disinfection experiments showed that 73% of E. coli O157:H7 died within 2 h with a disinfection rate

134 sensor was developed based on the ability of E. coli O157:H7 proteases to change the optical response

135 The colonizing ability of E. coli O157:H7 was compared with those of nonpathogenic

136 phere, and the higher number and activity of E. coli O157 cells in the rhizosphere may be a consequen

137 Adherence of E. coli O157 : H-expressing flagella of serotype H7, H6

138 and it contributes to intimate adherence of E. coli O157:H7 to the HEp-2 cell surface.

139 rotein and resulted in decreased adhesion of E. coli O157:H7.

140 indicate that GadE is critical for the AR of E. coli O157:H7 and that it plays an important role in v

141 The binding of E. coli O157:H7 to the IgM on B cell surface activates t

142 for enteric culture (0.7%), with 65 cases of E. coli O157.

143 The cells of E. coli O157:H7 specifically captured and enriched on th

144 e used to identify sublineages and clades of E. coli O157, and when they were correlated with the cli

145 Thus, StcE may help block host clearance of E. coli O157:H7 by destruction of some classes of glycop

146 ted in intestinal-epithelial colonization of E. coli O157:H7 and may represent a useful target for va

147 sal E. coli strains prevents colonization of E. coli O157:H7 in a mouse model.

148 Extremely low concentration of E. coli O157:H7 (~10 CFU/mL) could be detected within 1h

149 Concentrations of E. coli O157:H7 from 3x10(1) to 3x10(7)cfu/mL could be d

150 inoculated with different concentrations of E. coli O157:H7 have been tested using anti-E. coli O157

151 g mixture distribution analysis on counts of E. coli O157.

152 rect plating on SMACct, the direct counts of E. coli O157:H7 were highly correlated with the estimate

153 alysis of a mid-exponential-phase culture of E. coli O157 grown in LB broth.

154 The sensitivity of detection of E. coli O157 by both direct plating and IMS was highly d

155 Sensitivity of detection of E. coli O157 in bovine feces at low initial concentratio

156 n adequate stand-alone test for detection of E. coli O157 in clinical samples.

157 Finally, the sensitive detection of E. coli O157 in the presence of K12 at a ratio of 1:1000

158 1 and stx2) (including specific detection of E. coli O157), Shigella spp./enteroinvasive E. coli, Cry

159 is study, we reported the rapid detection of E. coli O157:H7 by using calcium signaling of the B cell

160 mical immunosensor for specific detection of E. coli O157:H7 contamination with the use of sandwich a

161 que and sensitive tools for the detection of E. coli O157:H7 from food as they are host-specific and

162 r phage assay shows promise for detection of E. coli O157:H7 from food in a simple, fast and sensitiv

163 The detection of E. coli O157:H7 in foods has held the attention of many

164 ased biosensor was used for the detection of E. coli O157:H7 in ground beef samples.

165 uffer pH, and assay time on the detection of E. coli O157:H7 were investigated and optimized.

166 E. coli O157:H7, the sensitive detection of E. coli O157:H7 were realized both in standard samples a

167 d bands and thus, enable visual detection of E. coli O157:H7 without instrumentation.

168 s highly desirable for specific detection of E. coli O157:H7, one of the leading bacterial pathogens

169 PAD) has been developed for the detection of E. coli O157:H7, Salmonella Typhimurium, and L. monocyto

170 ped for sensitive and selective detection of E. coli O157:H7.

171 typhimurium interfered with the detection of E. coli O157:H7.

172 for the visual and quantitative detection of E. coli O157:H7.

173 ensive studies of the global distribution of E. coli O157 lineages and the impacts of regionally pred

174 es evidence for the geographic divergence of E. coli O157 and for a prominent role of stx2a in total

175 idization and used to study the diversity of E. coli O157 isolates from human infections and food and

176 In particular, the capture of genomic DNA of E. coli O157:H7 by a specific oligonucleotide probe coat

177 eep were treated with biweekly oral doses of E. coli O157:H7 (an STEC) or an isogenic stx mutant stra

178 proved our understanding of the evolution of E. coli O157:H7 and suggested a key revision to accommod

179 f the plant can also influence the extent of E. coli O157 colonization.

180 hages may increase the pathogenic fitness of E. coli O157:H7.

181 logues of LpxR are present in the genomes of E. coli O157:H7, Yersinia enterocolitica, Helicobacter p

182 Using comparative genomics of E. coli O157:H7 subpopulations, we have identified one s

183 n and a complete inhibition of the growth of E. coli O157:H7 in the later 24 h irradiation.

184 or more rapid and accurate identification of E. coli O157:H7 in single-cell fluorescence-based assays

185 in a specific test for the identification of E. coli O157:H7, distinguishing it from other pathogenic

186 on and subsequent disease after ingestion of E. coli O157:H7 may depend, at least in part, on individ

187 g human consumption, leaf internalization of E. coli O157 may pose more of a public health risk than

188 ypic analyses of 120 independent isolates of E. coli O157:H7 from our culture collection revealed tha

189 and rapid technique to detect low levels of E. coli O157:H7 using membrane filtration and silver int

190 entify SLT-1 from the complex cell lysate of E. coli O157:H7.

191 ter inoculation onto plants, the majority of E. coli O157:H7 cells either die or are no longer cultur

192 thereby facilitating genetic manipulation of E. coli O157:H7.

193 lie the molecular pathogenetic mechanisms of E. coli O157:H7.

194 GA also cause injury to the cell membrane of E. coli O157:H7.

195 dality, was investigated in a mouse model of E. coli O157:H7 infection.

196 c ecf operon and/or lpxM deletion mutants of E. coli O157:H7 ATCC 43894 were constructed and analyzed

197 del of the regulation of the lpfA1 operon of E. coli O157:H7 by H-NS and Ler is discussed.

198 In 2006, in an outbreak of E. coli O157:H7 caused by consumption of contaminated sp

199 09, we investigated a multistate outbreak of E. coli O157:H7 infections.

200 The pathogenicity of E. coli O157:H7 is mainly caused by the expression of Sh

201 The pathogenicity of E. coli O157:H7 was also investigated in rabbits.

202 IgG against the O-specific polysaccharide of E. coli O157:H7 may confer immunity by lysing the inocul

203 hat bulk anise oil reduced the population of E. coli O157:H7 and L. monocytogenes by 1.48 and 0.47 lo

204 t Scotland were examined for the presence of E. coli O157.

205 Irrespective of the presence of E. coli O157:H7, TNF-alpha enhanced activation of p65, t

206 ably grossly underestimate the prevalence of E. coli O157 in cattle.

207 control measures to reduce the prevalence of E. coli O157.

208 7.5% to 22.5%, compared to the prevalence of E. coli O157:H7 that ranged from 0% to 15%.

209 servation even in low concentration range of E. coli O157:H7.

210 -PCR results prompted successful recovery of E. coli O157 (n = 25) and non-O157 STEC (n = 8) isolates

211 tract of cattle is the primary reservoir of E. coli O157:H7, and thus, it is critical to eliminate o

212 (2) The retention of E. coli O157:H7 was 3.3 fold higher than that of E. coli

213 detection limit, 100 CFU g(-1) dry soil) of E. coli O157:H7 was observed in the soils from Salinas V

214 . coli strains but absent from MS spectra of E. coli O157:H7 strains was identified by top-down analy

215 magnifying the imbalance of redox status of E. coli O157:H7.

216 tion of a nalidixic acid-resistant strain of E. coli O157 in bovine feces was assessed by culture on

217 ed with a chromosomally lux-marked strain of E. coli O157:H7.

218 cspC, ybgS, yahO, and yjbJ for 11 strains of E. coli O157:H7 and 7 strains of the "near-neighbor" ser

219 detection strategy for pathogenic strains of E. coli O157:H7 serotype based on a conserved signature

220 studies, we have determined that a subset of E. coli O157 infections will not be detected if an agar-

221 etection and epidemiological surveillance of E. coli O157, and the data were used to identify discern

222 suitable for epidemiological surveillance of E. coli O157.

223 a functional GAD system and for survival of E. coli O157:H7 in a simulated gastric environment.

224 nd EC significantly affected the survival of E. coli O157:H7 in leafy green producing soils and the d

225 n indirect effect in the overall survival of E. coli O157:H7 in soils.

226 n sediments, survival of FIB but not that of E. coli O157:H7 increased in disinfected treatments, ind

227 Here, the transmission of E. coli O157 within a typical UK dairy herd is modelled

228 wt % led to a reduction in the transport of E. coli O157:H7 and K12 from 98 to 10% and from 95 to 70

229 ar into QS columns enhanced the transport of E. coli O157:H7 by 3.1 fold compared to the unoxidized c

230 e 1 trial in adults showed that a vaccine of E. coli O157:H7 O-specific polysaccharide conjugated to

231 tribution of GadE to the AR and virulence of E. coli O157:H7 remains largely unknown.

232 ic microbiota could enhance the virulence of E. coli O157:H7, particularly a subset of clade 8 strain

233 inhibitory effect of C70-TiO2 thin films on E. coli O157:H7 showed a decrease of the bacterial conce

234 ns previously tested for Stx (by EIA) and/or E. coli O157:H7 (by culture) were tested by PCR.

235 Otherwise, E. coli O157:H7 only growth 50% when PBE was added to th

236 E. coli K-12 strains, as well as pathogenic E. coli O157:H7, exhibited compromised acid resistance i

237 ique enables digital detection of pathogenic E. coli O157 cells in a high background of normal K12 ce

238 cterial cell mixtures composed of pathogenic E. coli O157:H7 and harmless E. coli DH5alpha using flow

239 immunosensor for the detection of pathogenic E. coli O157:H7.

240 he electrical properties once the pathogenic E. coli O157:H7 captured on the sensor surface.

241 as the fluorophore label for the pathogenic E. coli O157:H7 serotype: limits of 1% O157:H7 in 99% DH

242 competitive advantage in vivo to pathogenic E. coli O157:H7 and commensal E. coli K-12, whereas degr

243 ultaneous testing of the foodborne pathogens E. coli O157:H7 and Salmonella enterica, in detail a nuc

244 e host inflammatory response acts to perturb E. coli O157:H7 intestinal colonization.

245 Once infected by the PhiV10 reporter phage, E. coli O157:H7 produces a strong bioluminescent signal

246 fy viable (with PMA) and total (without PMA) E. coli O157:H7 cells on growth chamber and field-grown

247 es and the impacts of regionally predominant E. coli O157 lineages on the prevalence and severity of

248 ), and EIID(Aga) of the Aga PTS are present, E. coli O157:H7 strains normally are able to utilize Aga

249 o virulence factors of Shiga toxin-producing E. coli O157:H7.

250 thus, it is critical to eliminate or reduce E. coli O157:H7 gut colonization.

251 ul component in a systemic vaccine to reduce E. coli O157:H7 colonization in cattle.

252 dition anise oil nanoemulsion (AO75) reduced E. coli O157:H7 and L. monocytogenes count by 2.51 and 1

253 o epithelial cells and significantly reduced E. coli O157 excretion levels from sheep.

254 ere found to be related to the two sequenced E. coli O157:H7 strains, EDL933 and Sakai.

255 eas closely related sorbitol-fermenting (SF) E. coli O157:H(-) strains carry plasmid pSFO157 (>120,00

256 Similarly, E. coli O157:H7 adhesion to cattle colonic explants was

257 e method is gram negative pathogenic species E. coli O157:H7.

258 chieved in less than 30 min at both species (E. coli O157:H7 vs S. typhimurium ) and strain (E. coli

259 l of Enterococcus faecalis, Salmonella spp., E. coli O157 and porcine parvovirus in bioreduction vess

260 ETEC], Shiga toxin-producing E. coli [STEC], E. coli O157:H7, Vibrio cholerae, Yersinia enterocolitic

261 coli O157:H7 vs S. typhimurium ) and strain (E. coli O157:H7 vs E. coli K12) levels in complex food m

262 extract and diallyl compounds treatment than E. coli O157:H7.

263 d non-O157 STEC in clinical samples and that E. coli O157:H7 remains the predominant cause of HUS in

264 Given that E. coli O157:H7 produces effectors that attenuate inflam

265 and stepwise regression analysis showed that E. coli O157:H7 survival in soils was negatively correla

266 The E. coli O157:H7 bacteriophage PhiV10 was modified to exp

267 The E. coli O157:H7 best able to persist had intimin, Tir, a

268 The E. coli O157:H7 strain EDL933 carries two copies of non-

269 trinsically curved DNAs were cloned from the E. coli O157:H7 virulence plasmid (pO157), sequenced and

270 ulator of the LEE, encoded by ecs1581 in the E. coli O157:H7 strain Sakai genome and present but not

271 tudy and are now available for typing of the E. coli O157:H7 lineage.

272 -NS) binds to the regulatory sequence of the E. coli O157:H7 lpf1 operon and "silences" its transcrip

273 better understanding of the evolution of the E. coli O157:H7 pathogenome, the present study presents

274 osidase assay results, we concluded that the E. coli O157:H7 lpf operon possesses a promoter dependen

275 imit of 0.2 cfu/microL is achieved using the E. coli O157 target and an input volume of 50 microL.

276 nsification of color was correlated with the E. coli O157:H7 concentration.

277 and the specific recognition of antibody to E. coli O157:H7, the sensitive detection of E. coli O157

278 was isolated, 28 (90%) were attributable to E. coli O157 and 3 (10%) were attributable to non-O157 S

279 t microvascular endothelial cells exposed to E. coli O157:H7-derived Stx2 and LPS release chemokines

280 a differential cultivar-specific response to E. coli O157 colonization, although importantly there wa

281 h graphene based capacitors were specific to E. coli O157:H7 strain with a sensitivity as low as 10-1

282 Unfortunately, E. coli O157 cannot be genetically manipulated using the

283 Enrichment assays using E. coli O157:H7 grown in LB broth with a reporter phage

284 hough the feasibility was demonstrated using E. coli O157:H7 as a model analyte, this approach could

285 The real-time monitoring method for viable E. coli O157:H7 developed in this study can be used to e

286 of NF-kappaB inflammatory signaling, whereas E. coli O157:H7 infection suppressed this pathway by inh

287 defined confirmed cases as persons from whom E. coli O157:H7 with the outbreak PFGE pattern was cultu

288 responsible for most deaths associated with E. coli O157 infection.

289 r treatment of complications associated with E. coli O157:H7 infection.

290 capacitors that were biofunctionalized with E. coli O157:H7 specific antibodies for sensitive pathog

291 Following subsequent oral challenge with E. coli O157:H7, reduced colonization rates and delayed

292 We measured Ang-1/2 in 77 children with E. coli O157:H7 infection.

293 Fifteen percent of patients infected with E. coli O157:H7 progress to hemolytic uremic syndrome, b

294 %) or low (2%) fiber diets and infected with E. coli O157:H7 strain 86-24 (Stx2+).

295 We enrolled children infected with E. coli O157:H7 within 1 week of the onset of diarrhea i

296 Finally, in children infected with E. coli O157:H7, plasma SDF-1 levels were elevated in in

297 occurs after gastrointestinal infection with E. coli O157:H7, which produces Shiga toxins (Stx) that

298 human and experimental bovine infection with E. coli O157:H7.

299 cient C57BL/6 mice were also inoculated with E. coli O157:H7 and only 1 of 14 developed disease, wher

300 In experiments using meats spiked with E. coli O157:H7, colicins efficiently reduced the popula