ライフサイエンスコーパス: E. faecium

1 E. faecium has been categorized as belonging to three cl

2 E. faecium is able to produce hydrogen peroxide by using

3 E. faecium isolates (n=577) from diverse sources were sc

4 E. faecium was isolated from 105 patients, 65 vegetarian

5 the United Kingdom and Ireland (UK&I) and 11 E. faecium isolates from a reference collection.

6 ly, antibodies to Acm were present in all 14 E. faecium endocarditis patient sera.

7 y of 181 Enterococcus faecalis isolates, 157 E. faecium isolates, and 60 isolates of other species; h

8 ophoresis (PFGE) revealed that all of the 19 E. faecium isolates with the VanB phenotype had identica

9 ded 108 (69%) E. faecalis isolates, 46 (29%) E. faecium isolates, and 1 isolate each of E. avium, E.

10 Despite the detection of acm in 32 out of 32 E. faecium isolates, only 11 of these (all clinical isol

11 . flavescens [n = 10], E. faecalis [n = 34], E. faecium [n = 43], E. avium [n = 1], E. gallinarum [n

12 aecium populations, we have now assessed 433 E. faecium isolates, including 264 isolates from human c

13 dertook whole-genome sequencing (WGS) of 495 E. faecium bloodstream isolates from 2001-2011 in the Un

14 rococcus faecalis isolates, 30 of 58 (51.7%) E. faecium isolates, 1 of 1 E. raffinosus isolate, 0 of

15 Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm

16 ve determined the structure of the activated E. faecium LiaR protein at 3.2A resolution and, in combi

17 lus three additional C. coli, one additional E. faecium, and one C. jejuni also developed resistance

18 ,4-triazolo[1,5-a]pyrimidines active against E. faecium is reported herein.

19 rrow-spectrum antibacterial activity against E. faecium and exhibited metabolic stability with low in

20 jor (VM) errors (4/14) with enterococci (all E. faecium) but none (0/22) with staphylococci.

21 ted as resistant by 100% of laboratories; an E. faecium strain with moderate-level resistance (MIC, 6

22 in, purified from culture supernatants of an E. faecium clinical isolate, was found to match the N-te

23 fied in Enterococcus faecium by screening an E. faecium genomic expression library with sera from pat

24 , and tetracycline resistance from C68 to an E. faecium recipient strain occurs at low frequency in v

25 r polysaccharides from E. faecalis 12030 and E. faecium 838970 were purified, and chemical and struct

26 Antimicrobial-resistant E. coli and E. faecium have become significant pathogens in oncology

27 ) and 3.1 x 10(1) CFU/mL or g of E. coli and E. faecium, respectively) sprout and water samples teste

28 own to effectively kill both E. faecalis and E. faecium (including vancomycin-resistant strains), as

29 icillin-imipenem tested with E. faecalis and E. faecium by BMD was >/=94% but was </=90% for other en

30 ntify enterococci other than E. faecalis and E. faecium can be compensated for by the addition of sta

31 The E. faecalis and E. faecium isolates differed in their susceptibilities t

32 species, we found that both E. faecalis and E. faecium kill C. elegans eggs and hatchlings, although

33 isolates and (10(0) CFU/mL) E. faecalis and E. faecium strains were detected within 4 and 8 h of pre

34 icillin-imipenem tested with E. faecalis and E. faecium was >/=98% and was 92% for other enterococci;

35 between vancomycin-resistant E. faecalis and E. faecium.

36 ), ST6 (n = 3), and ST185 (n = 1), which are E. faecium sequence types belonging to clonal complex 5

37 ntage of enterococci that were identified as E. faecium increased from 12.7 to 22.2% (P < 0.001) and

38 been previously phenotypically identified as E. faecium.

39 why antibiotic-resistant hospital-associated E. faecium are often replaced by clade B strains once pa

40 solates, are part of the hospital-associated E. faecium genogroup referred to as clonal complex 17 (C

41 ion and dissemination of hospital-associated E. faecium in the UK&I and provide evidence for WGS as a

42 by alternate methods, they were found to be E. faecium.

43 ned with brain heart infusion broth for both E. faecium U8304 and E. faecalis V583.

44 of identifying patients colonized with both E. faecium and E. faecalis, a feature useful for infecti

45 caused by E. faecalis and 37% were caused by E. faecium.

46 nts), at doses that promoted colonization by E. faecium C68.

47 hat promote gastrointestinal colonization by E. faecium have not been identified.

48 s suggest that hydrogen peroxide produced by E. faecium has cytotoxic effects and highlight the utili

49 analysis revealed that CVM1869 was a canine E. faecium clone that had acquired Tn1546, perhaps from

50 d E. faecalis, E. mundtii, E. casseliflavus, E. faecium, E. hirae, E. avium, and E. durans, respectiv

51 genes were frequently present in 30 clinical E. faecium isolates studied; one of these, acm, has been

52 ene was not identified in any of 34 clinical E. faecium isolates or in 4 other less pathogenic entero

53 high-level ampicillin resistance in clinical E. faecium strains.

54 butes to intestinal colonization in clinical E. faecium strains.

55 bility of a colonization-proficient clinical E. faecium isolate (C68) to transfer colonizing ability

56 r previous studies showed that some clinical E. faecium isolates produce a cell wall-anchored collage

57 are no reports experimentally demonstrating E. faecium virulence determinants.

58 olonization levels by strains from different E. faecium lineages: clade B, part of the healthy human

59 al biochemical methods fail to differentiate E. faecium from certain newly described enterococcal spe

60 We suggest that actively dividing E. faecium cells have three zones of unique peptidoglyca

61 tive virulence gene associated with epidemic E. faecium strains.

62 In conclusion, the extracelluar E. faecium SagA protein is apparently essential for grow

63 ococcus species, 633 strains of E. faecalis, E. faecium, and other enterococci isolated from blood cu

64 gative staphylococci, Enterococcus faecalis, E. faecium, E. avium, E. durans, E. casseliflavus, and E

65 including the species Enterococcus faecalis, E. faecium, E. casseliflavus, and E. gallinarum.

66 colonize humans, only Enterococcus faecalis, E. faecium, E. raffinosus, and E. casseliflavus have bee

67 istin-dalfopristin resistance in human fecal E. faecium.

68 For penicillin, three of five E. faecium strains but none of five Enterococcus faecali

69 e resistance development in four out of five E. faecium strains; however, increased resistance was ob

70 For E. faecium and other enterococci, the combination of FS

71 For E. faecium, categorical agreement was poor between the r

72 in-imipenem was 91% for E. faecalis, 98% for E. faecium, and 87% for other enterococci.

73 Genomes were also available for E. faecium associated with bloodstream infections in 15

74 ose for the reference method, especially for E. faecium isolates.

75 ggesting that the sagA gene is essential for E. faecium growth and may be involved in cell wall metab

76 m the first factor shown to be important for E. faecium pathogenesis.

77 owever, all the Emp pilins are important for E. faecium to cause infection in the urinary tract.

78 re 63%, 63%, 63%, and 56%, respectively, for E. faecium, 87%, 83%, 98%, and 80%, respectively, for E.

79 Rectal or fecal samples for E. faecium testing were obtained from 567 newly admitted

80 from 94 of these isolates into plasmid-free E. faecium GE-1 at transfer frequencies of <10(-9) to 10

81 y affinity-purified anti-Acm antibodies from E. faecium endocarditis patient sera, suggesting that Ac

82 ied four distinct surface carbohydrates from E. faecium endocarditis isolate Tx16, shown previously t

83 quence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all ot

84 The structure of VanX from E. faecium has been solved by X-ray crystallography and

85 -dalfopristin resistance was absent in human E. faecium, but 56% of conventional poultry isolates wer

86 We identified E. faecium transposon insertion mutants with altered C.

87 ritavancin have different modes of action in E. faecium.

88 Deletion of ahyD in E. faecium resulted in increased formation of Ala-PG and

89 tin-dalfopristin resistance were assessed in E. faecium isolates, and resistance genes were identifie

90 This gene is highly enriched in E. faecium clinical isolates and absent in commensal iso

91 and their involvement has been implicated in E. faecium as well.

92 PTS permease BepA is directly implicated in E. faecium pathogenesis.

93 2 excises to form a circular intermediate in E. faecium.

94 icin resistance and 1 for nitrofurantoin, in E. faecium isolates.

95 bition of transpeptidation by oritavancin in E. faecium is the result of the large number of secondar

96 When overexpressed in E. faecium and Escherichia coli, the native and recombin

97 ), encoded immediately adjacent to rakPGS in E. faecium, which is responsible for the hydrolysis of a

98 , we demonstrated a significant reduction in E. faecium collagen adherence by affinity-purified anti-

99 tial impact on colonization and virulence in E. faecium and possibly other Gram-positive bacterial sp

100 genesis of Gram-positive bacteria, including E. faecium We previously demonstrated that a nonpiliated

101 tomycin MICs of 3-4 microg/mL in the initial E. faecium blood isolate predicted microbiological failu

102 A comparison of the E. faecalis LiaR, E. faecium LiaR, and the LiaR homolog from Staphylococcu

103 in the clinical importance of multiresistant E. faecium across different continents.

104 d highly related vanA-positive and -negative E. faecium, which implies that control of vancomycin-res

105 transfer colonizing ability to noncolonizing E. faecium recipient strains.

106 anced surveillance and control of nosocomial E. faecium transmission and infection.

107 Correspondingly, E. faecalis, but not E. faecium, degrades the glycan on RNase B during growth

108 vatE was present in 38% of E. faecium isolates from patients and none from vegetari

109 pristin resistance was found in 51 to 78% of E. faecium isolates from the food production environment

110 imary adhesin responsible for the ability of E. faecium to bind collagen.

111 s PTS, significantly impaired the ability of E. faecium to colonize the murine intestinal tract durin

112 on/acquisition has favored the adaptation of E. faecium to nosocomial environments and/or patients.

113 emonstrate that Fnm affects the adherence of E. faecium to fibronectin and is important in the pathog

114 -fold decrease (P < 0.05) in the adhesion of E. faecium TX82 to fibronectin.

115 ould significantly enhance the attachment of E. faecium cells onto the surface of silica sands and th

116 The disparity in the transport behavior of E. faecium with and without esp could limit the effectiv

117 nfluence of esp on the transport behavior of E. faecium.

118 ctional acm gene is necessary for binding of E. faecium strains to CI.

119 native Acm is sufficient for the binding of E. faecium to CI.

120 nd this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon.

121 Integration of Tn5382 in the chromosome of E. faecium C68 has occurred 113 bp downstream of the sto

122 a nonpiliated DeltaempABC::cat derivative of E. faecium TX82 was attenuated in biofilm formation and

123 that Acm has contributed to the emergence of E. faecium and CC17 in nosocomial infections.

124 The frequency of E. faecium occurrence increased from 6% in the dry perio

125 ptococcus pneumoniae pavA, in the genomes of E. faecium strain TX82 and all other sequenced E. faeciu

126 We also show that CcpA affects the growth of E. faecium, that an intact ccpA gene is important for fu

127 implicating a role for CcpA in the growth of E. faecium.

128 as developed for the rapid identification of E. faecium.

129 ltransferase from a human urinary isolate of E. faecium, has been determined as an apoenzyme and in c

130 three collagen-binding clinical isolates of E. faecium tested, but in none of the strains with a non

131 in all other tests, while the 53 isolates of E. faecium were ARA positive only.

132 rtain infection-derived clinical isolates of E. faecium, and suggest that Acm is the primary adhesin

133 apeutic alternatives because the majority of E. faecium isolates are resistant to ampicillin and vanc

134 silica sands and thus lower the mobility of E. faecium within sand packs.

135 or country, indicating frequent movement of E. faecium between regions that rarely share hospital pa

136 were isolated during a hospital outbreak of E. faecium with plasmid-mediated VanB resistance.

137 Twenty-two percent of E. faecium isolates showed reduced susceptibility to qui

138 Both the percentage of E. faecium among the enterococci and the proportion of v

139 993 to 2002, we evaluated the percentages of E. faecium among all enterococcal isolates and the perce

140 enterococcal isolates and the percentages of E. faecium isolates that were vancomycin resistant.

141 c free) were also tested for the presence of E. faecium.

142 7 to 22.2% (P < 0.001) and the proportion of E. faecium that was vancomycin resistant increased from

143 c support for the tetracycline resistance of E. faecium 664.1H1 was characterized.

144 plain the effects of esp on the retention of E. faecium.

145 issemination of a highly resistant strain of E. faecium among patients and among other extant VRE str

146 e clonal dissemination of a single strain of E. faecium VanB.

147 Interestingly, a high titer of E. faecium also accumulates in the nematode gut, but doe

148 glycopeptide resistance element, Tn1546, of E. faecium 228 was used as the basis of comparison for a

149 cannot be eradicated by prolonged feeding on E. faecium.

150 One E. faecium isolate, CVM1869, displayed high-level resist

151 urrent study, one E. faecalis strain and one E. faecium strain carried only the vanC gene.

152 ts for 45 of 46 cultures with E. faecalis or E. faecium.

153 er of VR isolates, belonging to esp-positive E. faecium, was revealed.

154 nents of a glycoconjugate vaccine to prevent E. faecium infection.

155 enic strain overproducing catalase prevented E. faecium-mediated killing.

156 ) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and

157 early US AMP isolates were clonally related, E. faecium CC17 isolates have been circulating in the Un

158 , Lc-Lys could lyse the ampicillin-resistant E. faecium mutant with 3-->3 L-Lys(3)-D-Asn-L-Lys(3) bri

159 Quinupristin-dalfopristin-resistant E. faecium contaminates a large proportion of chickens s

160 Quinupristin-dalfopristin-resistant E. faecium was isolated from 237 chicken carcasses and 3

161 uency of quinupristin-dalfopristin-resistant E. faecium, we used selective medium to culture samples

162 riage of quinupristin-dalfopristin-resistant E. faecium.

163 se the potential for streptogramin-resistant E. faecium infection in humans.

164 al [CI], 6.20-8.76) and vancomycin-resistant E. faecium (rate ratio, 2.27, 95% CI, 2.03-2.53).

165 erms of the recovery of vancomycin-resistant E. faecium and E. faecalis, the sensitivity and PPV were

166 fferentiating IVRE from vancomycin-resistant E. faecium and E. faecalis; however, a combination of LM

167 m from three CI-binding vancomycin-resistant E. faecium clinical isolates showed 100% identity, analy

168 ee clinical isolates of vancomycin-resistant E. faecium collected from 3 patients during a 6-week per

169 rog/mL, show almost all vancomycin-resistant E. faecium have AUICs <125.

170 alfopristin, a drug for vancomycin-resistant E. faecium in humans.

171 i and the proportion of vancomycin-resistant E. faecium increased significantly over this 10-year per

172 One vancomycin-resistant E. faecium isolate was highly resistant to both teicopla

173 s the first report of a vancomycin-resistant E. faecium isolated from a companion animal in the Unite

174 in this study were all vancomycin-resistant E. faecium isolates collected from 28 hospitals and thre

175 were found primarily in vancomycin-resistant E. faecium isolates in nonstool cultures obtained from p

176 Nineteen (90%) of the vancomycin-resistant E. faecium isolates were of the VanB phenotype, with van

177 aecalis strain and on a vancomycin-resistant E. faecium strain.

178 spital dissemination of vancomycin-resistant E. faecium strains over a 6-year period in southeastern

179 faecalis strains and 7 vancomycin-resistant E. faecium strains possess shared capsular polysaccharid

180 calis and 4 isolates of vancomycin-resistant E. faecium that were not recovered by BEAV.

181 6, perhaps from a human vancomycin-resistant E. faecium.

182 to treat culture-proven vancomycin-resistant E. faecium.

183 faecium strain TX82 and all other sequenced E. faecium isolates.

184 We previously showed that some E. faecium strains express a cell wall-anchored collagen

185 e 16S rRNA sequences of enterococcal species E. faecium, E. faecalis, E. gallinarum, E. casseliflavus

186 These data suggest that specific E. faecium strains may be enriched in determinants that

187 d accurate detection of Staphylococcus spp., E. faecium, and E. faecalis and its ability to ascertain

188 nsferred DNA into a second recipient strain (E. faecium GE-1), which also colonized mice in significa

189 d be used cautiously against DAP-susceptible E. faecium strains with minimum inhibitory concentration

190 ires consideration of vancomycin-susceptible E. faecium Our findings reveal the evolution and dissemi

191 th vanA and vanB], 93 vancomycin-susceptible E. faecium) and epidemiological data were collected.

192 er mobility within saturated sand packs than E. faecium strains without esp.

193 an indicator of toxicity, we determined that E. faecium produces hydrogen peroxide at levels that cau

194 These results indicate that E. faecium clinical isolates express transmissible facto

195 We previously showed that E. faecium strains with daptomycin minimum inhibitory co

196 Overall, our results suggested that E. faecium strains with esp could display lower mobility

197 The E. faecium sagA gene, like the S. mutans homologue, is l

198 ber of colony-forming units per gram) by the E. faecium clades at any time point (P > .05).

199 of a secondary binding interaction with the E. faecium peptidoglycan.

200 ed d-Ala-d-Ala binding pocket still binds to E. faecium peptidoglycan.

201 ng 53 clinical and geographically diverse US E. faecium isolates dating from 1971 to 1994, we determi

202 ansconjugants derived from matings that used E. faecium D344SRF as a recipient strain colonized mouse

203 Using E. faecium-mediated killing of the nematode worm Caenorh

204 on of an altered Tn1546 in heterologous VanA E. faecium in Michigan hospitals.

205 s study shows that in a majority of the VanA E. faecium isolates, Tn1546 is altered compared to that

206 Variations among vanA and vanB E. faecium isolates from individual long-term-colonized

207 evalence of the other 14 genes among various E. faecium populations, we have now assessed 433 E. faec

208 VR E. faecium isolates were less genetically diverse than v

209 f VR E. faecalis isolates and none of the VR E. faecium isolates.

210 istration approval for the treatment of VRE (E. faecium) infections, namely, linezolid and quinuprist

211 terococci are Enterococcus faecalis, whereas E. faecium accounts for 5 to 10% of isolates.

212 to collagen, and this protein competed with E. faecium binding to immobilized CI.

213 United Kingdom identified 342 patients with E. faecium bloodstream infection over 7 years.

214 hort study that included adult patients with E. faecium BSI for whom initial isolates, follow-up bloo

215 We postulate that patients with E. faecium BSIs exhibiting daptomycin MICs of 3-4 microg

216 Thirty-seven of 41 sera from patients with E. faecium infections showed reactivity with recombinant

217 ression library with sera from patients with E. faecium-associated endocarditis.