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1                                              E. histolytica displays a relatively low level of nucleo
2                                              E. histolytica encodes a homolog of the human cytokine m
3                                              E. histolytica genes (and probably G. lamblia genes) enc
4                                              E. histolytica has been listed by the National Institute
5                                              E. histolytica HM-1:IMSS is a virulent strain, E. histol
6                                              E. histolytica infection appears to involve the initial
7                                              E. histolytica infection generally does not cause sympto
8                                              E. histolytica infection was established in human intest
9                                              E. histolytica infection was prevalent in this populatio
10                                              E. histolytica makes an unusual truncated N-glycan precu
11                                              E. histolytica may block the host inflammatory response
12                                              E. histolytica nuclear proteins exhibited sequence-speci
13                                              E. histolytica produces pore-forming proteins and protei
14                                              E. histolytica trophozoites colonize the colon, where th
15 y peaks were of higher amplitude following a E. histolytica infection compared to E. dispar (P = 0.01
16  Man(5)GlcNAc(2), which is the most abundant E. histolytica N-glycan, is aggregated into caps on the
17  for rheumatoid arthritis, as active against E. histolytica in culture.
18 optotic cells, monoclonal antibodies against E. histolytica membrane antigens were screened for inhib
19  Auranofin was ten times more potent against E. histolytica than metronidazole.
20 sized that the Q223 allele protected against E. histolytica via STAT3-mediated transcription of genes
21 icted immune responses in protection against E. histolytica infection.
22 to determine if calreticulin functions as an E. histolytica C1q receptor during phagocytosis of host
23 st one-hybrid screen was used to identify an E. histolytica cDNA encoding a protein (URE3-BP) that re
24 ment of amebic liver abscess formation in an E. histolytica infected hamster model.
25  silence multiple amebic genes, including an E. histolytica Myb gene, which is upregulated during oxi
26                    Recently, a segment of an E. histolytica gene was identified that encoded a protei
27 nd the mechanism of phagocytosis, we used an E. histolytica Affymetrix microarray chip to measure the
28                                   We used an E. histolytica DNA microarray consisting of 2,110 genes
29 (49%), Clostridium acetobutylicum (44%), and E. histolytica (43%) and lesser identities to the class
30  we named E. histolytica ILWEQ (EhILWEQ) and E. histolytica BAR (EhBAR), were chosen for localization
31 dispar, and 14 with both G. intestinalis and E. histolytica and/or E. dispar.
32 f host cell engulfment, or phagocytosis, and E. histolytica phagocytosis alters amebic gene expressio
33 ated with a reduced virulence phenotype, and E. histolytica HM-1:IMSS trophozoites infecting human in
34 M-1:IMSS, the prototype virulent strain, and E. histolytica Rahman, a strain that was reportedly less
35 om amebiasis is associated with mucosal anti-E. histolytica Gal/GalNAc lectin antibodies, suggesting
36            Nine of 12 (75%) people with anti-E. histolytica IgG also had EhMSP-1-specific IgG antibod
37                                           As E. histolytica does not readily form cysts in vitro, we
38 d with an increase in phagocytic ability, as E. histolytica cultures exposed to an initial stimulus o
39 ermine vaccine efficacy against asymptomatic E. histolytica intestinal infection.
40 ive lectin protein on the surfaces of axenic E. histolytica trophozoites or from solubilized amebae.
41 histolytica and human intestine, and between E. histolytica and hepatocytes, and discuss what these s
42 able of detecting and discriminating between E. histolytica, Entamoeba dispar, G. lamblia assemblages
43 rize recent work on the interactions between E. histolytica and human intestine, and between E. histo
44 system for studying the interactions between E. histolytica and human intestine.
45 s are found at syntenic break points between E. histolytica and E. dispar and hence, could work as re
46  this pathway may be the 97-kDa bifunctional E. histolytica alcohol dehydrogenase 2 (EhADH2), which p
47 s (95% CI, 2.02-50.6) more likely to be both E. histolytica negative and serum anti-lectin immunoglob
48 nce of a PS receptor on the surfaces of both E. histolytica and E. dispar.
49 he glycosomal ATP-PFK of Trypanosoma brucei, E. histolytica pfk1, and a second sequence from B. burgd
50 CID mouse model of amebic liver abscess, but E. histolytica trophozoites that do not express amoebapo
51 ba species while ERE2 was likely acquired by E. histolytica after its separation from E. dispar.
52 ide blocked Jurkat cell apoptosis induced by E. histolytica.
53 tor polymorphisms on intestinal infection by E. histolytica.
54 exin V prior to adherence to or ingestion by E. histolytica.
55         Prior to phagocytosis of host cells, E. histolytica induces apoptotic host cell death, using
56     In this study, we sought to characterize E. histolytica metallosurface protease 1 (EhMSP-1).
57 use of bloody diarrhea in Bolivian children; E. histolytica is uncommon.
58 2 h postchallenge, all Q223 mice had cleared E. histolytica, whereas 39% of 223R mice were infected.
59 Fv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biolo
60                               In conclusion, E. histolytica infection was confirmed in 9 (15.8%) of t
61 ed flanking regions containing the conserved E. histolytica transcription promoter elements located 5
62      Only 1 of 133 stool specimens contained E. histolytica trophozoites.
63                                 In contrast, E. histolytica possesses only single genes encoding NifS
64 amples spiked with serially diluted cultured E. histolytica trophozoites.
65 e whether the genes encoding these cytosolic E. histolytica fermentation enzymes might derive from a
66 ound that the E. histolytica II test detects E. histolytica infections more accurately.
67 ility of each assay to correctly distinguish E. histolytica from E. dispar was evaluated with DNA ext
68 ce calreticulin as a receptor for C1q during E. histolytica phagocytosis of host cells.
69  of cDNA ends were used to obtain the entire E. histolytica hsp60 coding region, which predicted a 53
70 mediated TGS in the deep-branching eukaryote E. histolytica.
71                                     Finally, E. histolytica calreticulin bound specifically to apopto
72  parvum or C. hominis, and 100% and 100% for E. histolytica, respectively.
73  domain of C1q were all chemoattractants for E. histolytica.
74 tolityca) and microarray expression data for E. histolytica.
75 ngladeshi children intensively monitored for E. histolytica infection for a 3-year period.
76  bacteria, the usual source of nutrients for E. histolytica.
77  indicate that EhADH2 enzyme is required for E. histolytica growth and survival and that the C-termin
78 trate that EhADH2 expression is required for E. histolytica growth and survival.
79 ia, 170, 95.9%, and 97.4%, respectively; for E. histolytica/E. dispar, 99, 96.0%, and 99.1%, respecti
80 ansfer, as has previously been suggested for E. histolytica genes encoding heat shock protein 60, nic
81                             The CBDs of four E. histolytica lectins (Jacob, chitinase, and Jessies 2
82 eoside diphosphate forming) were cloned from E. histolytica, and their evolutionary origins, as well
83 xcess K(+) protected diverse cell types from E. histolytica-induced death.
84 analyzed the ability of the third-generation E. histolytica Quik Chek assay developed by Techlab to d
85                    Despite its large genome, E. histolytica encodes only one rhomboid (EhROM1) with r
86                             Insight into how E. histolytica responds to oxidative stress increases ou
87                                           In E. histolytica trophozoites, EhROM1 changed localization
88 ifferent kinetics of ubiquitin activation in E. histolytica.
89 es in encysting E. invadens parasites and in E. histolytica trophozoites overexpressing chitinase und
90     The lack of a defined Golgi apparatus in E. histolytica as well as in other protists led to the h
91  well as in the phagolysosomal biogenesis in E. histolytica and thus contributes to the pathogenicity
92 tly novel role in transcriptional control in E. histolytica.
93 om a significantly higher leukocyte count in E. histolytica-positive patients than in negative patien
94 l relevance of raft-like membrane domains in E. histolytica.
95                  Overexpression of EhEBP1 in E. histolytica trophozoites resulted in a 7-fold drop in
96 ing the initiation of erythrophagocytosis in E. histolytica.
97 ate phagocytosis-related signaling events in E. histolytica have been characterized, the functions of
98 dispar and 32 genes with lower expression in E. histolytica Rahman than in E. histolytica HM-1:IMSS.
99 t mechanism of control of gene expression in E. histolytica.
100                  Motility is a key factor in E. histolytica pathogenesis, and this process relies on
101 ew stress-responsive transcription factor in E. histolytica that controls a transcriptional regulator
102 n the early stages of phagosome formation in E. histolytica.
103 idence for Golgi apparatus-like functions in E. histolytica as well as for components of glycoprotein
104  found a putative transposase-coding gene in E. histolytica and E. dispar related to the mariner tran
105  repressive histone mark to be identified in E. histolytica, dimethylation of H3K27 (H3K27Me2), and d
106 fic DNA binding activity to be identified in E. histolytica, should provide new insights into transcr
107  conserved mode of E2/RING-E3 interaction in E. histolytica.
108  allow for a unique polyubiquitin linkage in E. histolytica.
109 loped a new tool for genetic manipulation in E. histolytica with many advantages over currently avail
110 delay in erythrophagocytosis was observed in E. histolytica cells overexpressing S428A and KDDeltaC p
111 oteinase, EhCP5, which is functional only in E. histolytica.
112 ttle is known about the role of PI(4,5)P2 in E. histolytica pathogenicity.
113 ngly implicate the SREHP as a participant in E. histolytica phagocytosis and suggest that it may play
114 he potential for novel metabolic pathways in E. histolytica may allow for the development of new chem
115 -wide transcriptional regulatory patterns in E. histolytica.
116 bution during various endocytic processes in E. histolytica.
117 f PI3-kinase signaling in these processes in E. histolytica.
118 derstanding of the role of these proteins in E. histolytica virulence.
119  demonstrate that G3-based gene silencing in E. histolytica is mediated by an siRNA pathway, which ut
120 n in the nonvirulent species/strains than in E. histolytica HM-1:IMSS.
121  expression in E. histolytica Rahman than in E. histolytica HM-1:IMSS.
122           Together, our data suggest that in E. histolytica, PI(4,5)P2 may signal from lipid rafts an
123     The discovery of amoebic trogocytosis in E. histolytica may also shed light on an evolutionarily
124 the mechanism is not very well understood in E. histolytica.
125 ted overexpression of calreticulin increased E. histolytica's ability to phagocytose apoptotic lympho
126                           Despite induction, E. histolytica trophozoites were found to be resistant t
127               During the process of invasion E. histolytica ingests red blood and host cells using ph
128 ocytosis of host cells characterize invasive E. histolytica infection.
129 immunoassay for the detection of G. lamblia, E. histolytica/E. dispar, and C. parvum in fresh or fres
130  parasitic infections other than G. lamblia, E. histolytica/E. dispar, or C. parvum are suspected.
131               We have cloned the full-length E. histolytica gene encoding one such protein, chaperoni
132 imony analyses also suggested as less likely E. histolytica POR sharing more recent common ancestry w
133 ssion and shape the colonic microenvironment E. histolytica infects.
134 e, we have cloned and characterized multiple E. histolytica ubiquitination components, spanning ubiqu
135 of the five encoded proteins, which we named E. histolytica ILWEQ (EhILWEQ) and E. histolytica BAR (E
136 ecombinant protein, we have localized native E. histolytica CPN60 to a previously undescribed organel
137 ed with E. histolytica; the incidence of new E. histolytica infections in controls (as determined by
138 arkedly underestimated the occurrence of new E. histolytica infections, as 15.3% of controls seroconv
139 olytica-negative children but in only 34% of E. histolytica-positive children (overall odds ratio, 2.
140 nd homozygous genotypes were found in 55% of E. histolytica-negative children but in only 34% of E. h
141                               The ability of E. histolytica to phagocytose host cells correlates with
142 een shown previously to mediate adherence of E. histolytica to mammalian epithelial cells.
143 ition of the galactose-specific adherence of E. histolytica trophozoites to Chinese hamster ovary cel
144                  Interestingly, adherence of E. histolytica trophozoites to CHO cells was inhibited b
145 onal antibody to a 29-kDa surface antigen of E. histolytica bound to trophozoites 83.5% +/- 6.7% less
146 ects of which centre on expanding aspects of E. histolytica's metabolic repertoire.
147 olvement of PTPases during the attachment of E. histolytica to target cells.
148 nes exhibited the features characteristic of E. histolytica genes, such as transcripts with relativel
149  peaks that are associated with clearance of E. histolytica and E. dispar infection.
150 ta set demonstrating feed-forward control of E. histolytica phagocytosis.
151 rrelated with our finding that co-culture of E. histolytica trophozoites with mucin-producing T84 cel
152 a robust assay for the specific detection of E. histolytica trophozoites in unfixed frozen clinical s
153                    Core promoter elements of E. histolytica protein encoding genes include a TATA-lik
154 ildren in the cohort had a second episode of E. histolytica infection during the study period.
155                   The early establishment of E. histolytica in the colon occurs in the presence of an
156 nth that of the 60-kDa enzyme in extracts of E. histolytica trophozoites.
157 tive without prior activation in extracts of E. histolytica.
158             To identify virulence factors of E. histolytica, we first defined the phenotypes of two E
159                              The 6-kDa FD of E. histolytica and G. lamblia were most similar to those
160                     The transmissive form of E. histolytica is the cyst, with a single infected indiv
161 estinal cathelicidins is a novel function of E. histolytica cysteine proteinases in the evasion of th
162 es suggested that the adh1 and adhe genes of E. histolytica and Gram-positive eubacteria share a comm
163                Here we present the genome of E. histolytica, which reveals a variety of metabolic ada
164                         Complex N-glycans of E. histolytica are made by the addition of alpha1,2-link
165 ycan biosynthesis and the final N-glycans of E. histolytica with the following conclusions.
166 prospectively studied the natural history of E. histolytica colonization and diarrhea among infants i
167 ecently been published for identification of E. histolytica and differentiation from the morphologica
168 ved unsatisfactory for the identification of E. histolytica, E. coli, and C. mesnili.
169  vitro study demonstrated that incubation of E. histolytica trophozoites with epithelial cell lines r
170 bscesses following intrahepatic injection of E. histolytica by a combined rate of 68% in two independ
171 nate host immune response during invasion of E. histolytica, the protozoan cause of human amebiasis.
172  mABs generated against the 170-kD lectin of E. histolytica.
173 teady-state levels in the plasma membrane of E. histolytica and that these levels, unlike those in ma
174    Within the past four years, new models of E. histolytica infection have begun to illuminate how am
175 ted beads was disrupted by overexpression of E. histolytica p21(racA-V12), a ras-family protein invol
176 udies have demonstrated a high prevalence of E. histolytica.
177 extracellular neutral cysteine proteinase of E. histolytica trophozoites, one of the first amebic pro
178            A chromatographic purification of E. histolytica nuclear extracts by gel filtration, catio
179 d environmental factors in the regulation of E. histolytica virulence.
180 erse effects(3), and potential resistance of E. histolytica to the drug is an increasing concern(4,5)
181            Here, we investigated the role of E. histolytica MIF (EhMIF) during infection.
182                        The seroprevalence of E. histolytica was 33% (14/43) from the available stored
183                               All strains of E. histolytica express a 260-kDa surface Gal/GalNAc lect
184                 Several different strains of E. histolytica were found to contain multiple hgl loci i
185 n, is aggregated into caps on the surface of E. histolytica by the N-glycan-specific, anti-retroviral
186 Calreticulin was localized to the surface of E. histolytica during interaction with both Jurkat lymph
187 d to control animals bound to the surface of E. histolytica trophozoites and accelerated amebic lysis
188 th PI(4,5)P2 were enhanced upon treatment of E. histolytica cells with cholesterol.
189 thogenicity factors by which trophozoites of E. histolytica exert their remarkable cytolytic and tiss
190                             The virulence of E. histolytica correlates with the degree of host cell e
191 roteases degrade the key adherence lectin on E. histolytica trophozoites and decrease their adherence
192  false negatives by O&P (two G. lamblia, one E. histolytica/E. dispar, and one C. parvum).
193 preschool age, for infection by the parasite E. histolytica every other day over 9 years and evaluate
194 ose expression was upregulated in phagocytic E. histolytica trophozoites to determine whether these g
195                                The predicted E. histolytica POR showed fewer positional identities to
196                                      A prior E. histolytica infection was associated with the occurre
197                                    Recently, E. histolytica trophozoites that are totally deficient i
198                              The serine-rich E. histolytica protein (SREHP) is a surface-expressed tr
199 ression of the GalNAc lectin and serine-rich E. histolytica protein (SREHP) receptors.
200 antigen family that includes the serine-rich E. histolytica protein (SREHP), an amebic vaccine candid
201 ography-mass spectrometry as the serine-rich E. histolytica protein (SREHP).
202  of a liver transplant recipient with severe E. histolytica colitis who was successfully treated with
203 C. parvum or C. hominis) or trichrome stain (E. histolytica).
204 s opsonins on apoptotic cells that stimulate E. histolytica phagocytosis, an effect mediated at least
205 at the collectins are ligands that stimulate E. histolytica phagocytosis, we used a flow cytometry-ba
206  histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entam
207                                  In summary, E. histolytica N-glycans include unprocessed Man(5)GlcNA
208                       These proteins, termed E. histolytica enhancer-binding proteins 1 and 2 (EhEBP1
209                          We demonstrate that E. histolytica and E. dispar share their entire repertoi
210     Together, these studies demonstrate that E. histolytica has different vesicles that play a role i
211 nesis of amebic colitis, we demonstrate that E. histolytica trophozoites or their released proteinase
212 /BL6.lpr and C57/BL6.gld mice, we found that E. histolytica-induced apoptosis does not require the Fa
213 s to be at variance with the hypothesis that E. histolytica rather recently lost its mitochondrial or
214   The pattern of polymorphism indicates that E. histolytica reproduces sexually, or has done so in th
215                         Here, we report that E. histolytica induces apoptosis in both inflammatory ce
216 s factor receptor I gene, we have shown that E. histolytica-induced apoptosis is not mediated by tumo
217 in a mouse model of disease and suggest that E. histolytica induces cell death without using two comm
218                   These results suggest that E. histolytica-mediated host cell death occurs by a mech
219 ry of the sequenced genomes, suggesting that E. histolytica may reproduce sexually.
220                                          The E. histolytica acetyl-CoA synthetase, which converts ace
221                                          The E. histolytica genome contains two homologues to the met
222                                          The E. histolytica genome encodes several Rho family GTPases
223                                          The E. histolytica hsp60 gene protected from heat shock Esch
224                                          The E. histolytica Hsp60, which lacked characteristic carbox
225                                          The E. histolytica HSP70 is most similar to the higher eukar
226                                          The E. histolytica malic enzyme, which decarboxylates malate
227                                          The E. histolytica por gene encodes a 1,620-amino-acid pepti
228 tolytica microplate assay from Remel and the E. histolytica II enzyme-linked immunosorbent assay (ELI
229 dascreen Entamoeba test (R-Biopharm) and the E. histolytica II test (Techlab), and we found that the
230 mpared to the ProSpecT microplate assay, the E. histolytica Quik Chek (Quik Chek) assay exhibited 97.
231                              Conversely, the E. histolytica plasma membrane Gal/GalNAc lectin bound s
232 roaches we have (a) cloned and expressed the E. histolytica UDP-galactose transporter in Saccharomyce
233       The URE3-BP protein was present in the E. histolytica nucleus and cytoplasm with an apparent mo
234 al gene transfer of bacterial genes into the E. histolytica genome, the effects of which centre on ex
235 lls and inhibited neutrophil influx into the E. histolytica-infected intestinal xenografts.
236 alyses inferred a closer relationship of the E. histolytica ADHE to bacterial ADHE than to the G. lam
237  8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lecti
238 esent and significant in the function of the E. histolytica fdx gene promoter.
239 e composition bias and repetitiveness of the E. histolytica genome provide a challenge for short-read
240 tage process has been the publication of the E. histolytica genome, from which has come an explosion
241 d rafts, and the actin-rich fractions of the E. histolytica membrane.
242 under a promoter (g34) taken from one of the E. histolytica ribosomal protein (RP-L21) gene copies.
243        However, no functional studies of the E. histolytica ubiquitination enzymes have yet emerged.
244             After discrepant resolution, The E. histolytica Quik Chek assay exhibited sensitivity and
245                           In this study, the E. histolytica por gene and the adhE gene of a second am
246  assays suggested that auranofin targets the E. histolytica thioredoxin reductase, preventing the red
247 ica II test (Techlab), and we found that the E. histolytica II test detects E. histolytica infections
248 ort in Entamoeba is strong evidence that the E. histolytica organelle housing chaperonin CPN60 repres
249  sensitivity and specificity compared to the E. histolytica II ELISA of 98.0% (95% confidence interva
250             In this study, we compared three E. histolytica real-time PCR techniques published by Dec
251                                        Thus, E. histolytica infection was confirmed in 9 cases (15.8%
252  monoclonal antibody, following adherence to E. histolytica.
253 th EhILWEQ and EhBAR appear to contribute to E. histolytica virulence through their function in phago
254 tation enzymes from archaea or eubacteria to E. histolytica probably occurred early, because the sequ
255 that contributes to resistance of the gut to E. histolytica infection.
256                 Determination of immunity to E. histolytica following cure of ALA will require the us
257  discrete mechanisms of innate resistance to E. histolytica depending on the host background and, in
258 he human intestinal IgA antibody response to E. histolytica and E. dispar infection and revealed that
259 ction of IL-1 and IL-8 occurs in response to E. histolytica infection in vivo and as an approach to s
260 rophil-mediated tissue damage in response to E. histolytica infection; this inflammatory cascade can
261 ptin function may increase susceptibility to E. histolytica infection.
262 ion of this subunit were more susceptible to E. histolytica infection as measured by culture results,
263 ies-specific manner, while ERE2 is unique to E. histolytica.
264 tica, we first defined the phenotypes of two E. histolytica strains, HM-1:IMSS, the prototype virulen
265 ted were active (IC(50) < 200 microM) versus E. histolytica growth in vitro.
266                    Transfectants of virulent E. histolytica strain HM-1:IMSS, in which the expression
267 onvirulent species/strains than the virulent E. histolytica HM-1:IMSS.
268 tinal xenografts were infected with virulent E. histolytica trophozoites.
269                                         When E. histolytica trophozoites invade the lamina propria of
270 the sera of children living in an area where E. histolytica infection is endemic.
271 tica genome, raising the question of whether E. histolytica MIF (EhMIF) has proinflammatory activity
272 were diagnosed with G. intestinalis, 53 with E. histolytica and/or E. dispar, and 14 with both G. int
273  Statistically significant associations with E. histolytica and G. intestinalis were not found.
274                                 Contact with E. histolytica parasites triggered K(+) channel activati
275 oximately 80% of children were infected with E. histolytica by the age of 2 years.
276 l xenografts in SCID mice were infected with E. histolytica trophozoites expressing an antisense mess
277 grow; the xenografts were then infected with E. histolytica trophozoites.
278  from South Africans naturally infected with E. histolytica were examined by enzyme-linked immunosorb
279 eropositive subjects that were infected with E. histolytica, disease free, and asymptomatic.
280 at codon 223 were intracecally infected with E. histolytica.
281 udy, 6.3% of ALA subjects were infected with E. histolytica; the incidence of new E. histolytica infe
282                               Infection with E. histolytica increased prostaglandin E(2) (PGE2) level
283                               Infection with E. histolytica resulted in the expression of cyclooxygen
284 of isolated crypts from mice inoculated with E. histolytica.
285 re found to be resistant to reinfection with E. histolytica.
286 nd tissue damage that are normally seen with E. histolytica colonic infection.
287                 Infection of xenografts with E. histolytica trophozoites resulted in extensive tissue

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