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1 E. histolytica displays a relatively low level of nucleo
2 E. histolytica encodes a homolog of the human cytokine m
3 E. histolytica genes (and probably G. lamblia genes) enc
4 E. histolytica has been listed by the National Institute
5 E. histolytica HM-1:IMSS is a virulent strain, E. histol
6 E. histolytica infection appears to involve the initial
7 E. histolytica infection generally does not cause sympto
8 E. histolytica infection was established in human intest
9 E. histolytica infection was prevalent in this populatio
10 E. histolytica makes an unusual truncated N-glycan precu
11 E. histolytica may block the host inflammatory response
12 E. histolytica nuclear proteins exhibited sequence-speci
13 E. histolytica produces pore-forming proteins and protei
14 E. histolytica trophozoites colonize the colon, where th
15 y peaks were of higher amplitude following a E. histolytica infection compared to E. dispar (P = 0.01
16 Man(5)GlcNAc(2), which is the most abundant E. histolytica N-glycan, is aggregated into caps on the
18 optotic cells, monoclonal antibodies against E. histolytica membrane antigens were screened for inhib
20 sized that the Q223 allele protected against E. histolytica via STAT3-mediated transcription of genes
22 to determine if calreticulin functions as an E. histolytica C1q receptor during phagocytosis of host
23 st one-hybrid screen was used to identify an E. histolytica cDNA encoding a protein (URE3-BP) that re
25 silence multiple amebic genes, including an E. histolytica Myb gene, which is upregulated during oxi
27 nd the mechanism of phagocytosis, we used an E. histolytica Affymetrix microarray chip to measure the
29 (49%), Clostridium acetobutylicum (44%), and E. histolytica (43%) and lesser identities to the class
30 we named E. histolytica ILWEQ (EhILWEQ) and E. histolytica BAR (EhBAR), were chosen for localization
32 f host cell engulfment, or phagocytosis, and E. histolytica phagocytosis alters amebic gene expressio
33 ated with a reduced virulence phenotype, and E. histolytica HM-1:IMSS trophozoites infecting human in
34 M-1:IMSS, the prototype virulent strain, and E. histolytica Rahman, a strain that was reportedly less
35 om amebiasis is associated with mucosal anti-E. histolytica Gal/GalNAc lectin antibodies, suggesting
38 d with an increase in phagocytic ability, as E. histolytica cultures exposed to an initial stimulus o
40 ive lectin protein on the surfaces of axenic E. histolytica trophozoites or from solubilized amebae.
41 histolytica and human intestine, and between E. histolytica and hepatocytes, and discuss what these s
42 able of detecting and discriminating between E. histolytica, Entamoeba dispar, G. lamblia assemblages
43 rize recent work on the interactions between E. histolytica and human intestine, and between E. histo
45 s are found at syntenic break points between E. histolytica and E. dispar and hence, could work as re
46 this pathway may be the 97-kDa bifunctional E. histolytica alcohol dehydrogenase 2 (EhADH2), which p
47 s (95% CI, 2.02-50.6) more likely to be both E. histolytica negative and serum anti-lectin immunoglob
49 he glycosomal ATP-PFK of Trypanosoma brucei, E. histolytica pfk1, and a second sequence from B. burgd
50 CID mouse model of amebic liver abscess, but E. histolytica trophozoites that do not express amoebapo
58 2 h postchallenge, all Q223 mice had cleared E. histolytica, whereas 39% of 223R mice were infected.
59 Fv to each specifically detect their cognant E. histolytica cyst antigens was demonstrated in a biolo
61 ed flanking regions containing the conserved E. histolytica transcription promoter elements located 5
65 e whether the genes encoding these cytosolic E. histolytica fermentation enzymes might derive from a
67 ility of each assay to correctly distinguish E. histolytica from E. dispar was evaluated with DNA ext
69 of cDNA ends were used to obtain the entire E. histolytica hsp60 coding region, which predicted a 53
77 indicate that EhADH2 enzyme is required for E. histolytica growth and survival and that the C-termin
79 ia, 170, 95.9%, and 97.4%, respectively; for E. histolytica/E. dispar, 99, 96.0%, and 99.1%, respecti
80 ansfer, as has previously been suggested for E. histolytica genes encoding heat shock protein 60, nic
82 eoside diphosphate forming) were cloned from E. histolytica, and their evolutionary origins, as well
84 analyzed the ability of the third-generation E. histolytica Quik Chek assay developed by Techlab to d
89 es in encysting E. invadens parasites and in E. histolytica trophozoites overexpressing chitinase und
90 The lack of a defined Golgi apparatus in E. histolytica as well as in other protists led to the h
91 well as in the phagolysosomal biogenesis in E. histolytica and thus contributes to the pathogenicity
93 om a significantly higher leukocyte count in E. histolytica-positive patients than in negative patien
97 ate phagocytosis-related signaling events in E. histolytica have been characterized, the functions of
98 dispar and 32 genes with lower expression in E. histolytica Rahman than in E. histolytica HM-1:IMSS.
101 ew stress-responsive transcription factor in E. histolytica that controls a transcriptional regulator
103 idence for Golgi apparatus-like functions in E. histolytica as well as for components of glycoprotein
104 found a putative transposase-coding gene in E. histolytica and E. dispar related to the mariner tran
105 repressive histone mark to be identified in E. histolytica, dimethylation of H3K27 (H3K27Me2), and d
106 fic DNA binding activity to be identified in E. histolytica, should provide new insights into transcr
109 loped a new tool for genetic manipulation in E. histolytica with many advantages over currently avail
110 delay in erythrophagocytosis was observed in E. histolytica cells overexpressing S428A and KDDeltaC p
113 ngly implicate the SREHP as a participant in E. histolytica phagocytosis and suggest that it may play
114 he potential for novel metabolic pathways in E. histolytica may allow for the development of new chem
119 demonstrate that G3-based gene silencing in E. histolytica is mediated by an siRNA pathway, which ut
123 The discovery of amoebic trogocytosis in E. histolytica may also shed light on an evolutionarily
125 ted overexpression of calreticulin increased E. histolytica's ability to phagocytose apoptotic lympho
129 immunoassay for the detection of G. lamblia, E. histolytica/E. dispar, and C. parvum in fresh or fres
130 parasitic infections other than G. lamblia, E. histolytica/E. dispar, or C. parvum are suspected.
132 imony analyses also suggested as less likely E. histolytica POR sharing more recent common ancestry w
134 e, we have cloned and characterized multiple E. histolytica ubiquitination components, spanning ubiqu
135 of the five encoded proteins, which we named E. histolytica ILWEQ (EhILWEQ) and E. histolytica BAR (E
136 ecombinant protein, we have localized native E. histolytica CPN60 to a previously undescribed organel
137 ed with E. histolytica; the incidence of new E. histolytica infections in controls (as determined by
138 arkedly underestimated the occurrence of new E. histolytica infections, as 15.3% of controls seroconv
139 olytica-negative children but in only 34% of E. histolytica-positive children (overall odds ratio, 2.
140 nd homozygous genotypes were found in 55% of E. histolytica-negative children but in only 34% of E. h
143 ition of the galactose-specific adherence of E. histolytica trophozoites to Chinese hamster ovary cel
145 onal antibody to a 29-kDa surface antigen of E. histolytica bound to trophozoites 83.5% +/- 6.7% less
148 nes exhibited the features characteristic of E. histolytica genes, such as transcripts with relativel
151 rrelated with our finding that co-culture of E. histolytica trophozoites with mucin-producing T84 cel
152 a robust assay for the specific detection of E. histolytica trophozoites in unfixed frozen clinical s
161 estinal cathelicidins is a novel function of E. histolytica cysteine proteinases in the evasion of th
162 es suggested that the adh1 and adhe genes of E. histolytica and Gram-positive eubacteria share a comm
166 prospectively studied the natural history of E. histolytica colonization and diarrhea among infants i
167 ecently been published for identification of E. histolytica and differentiation from the morphologica
169 vitro study demonstrated that incubation of E. histolytica trophozoites with epithelial cell lines r
170 bscesses following intrahepatic injection of E. histolytica by a combined rate of 68% in two independ
171 nate host immune response during invasion of E. histolytica, the protozoan cause of human amebiasis.
173 teady-state levels in the plasma membrane of E. histolytica and that these levels, unlike those in ma
174 Within the past four years, new models of E. histolytica infection have begun to illuminate how am
175 ted beads was disrupted by overexpression of E. histolytica p21(racA-V12), a ras-family protein invol
177 extracellular neutral cysteine proteinase of E. histolytica trophozoites, one of the first amebic pro
180 erse effects(3), and potential resistance of E. histolytica to the drug is an increasing concern(4,5)
185 n, is aggregated into caps on the surface of E. histolytica by the N-glycan-specific, anti-retroviral
186 Calreticulin was localized to the surface of E. histolytica during interaction with both Jurkat lymph
187 d to control animals bound to the surface of E. histolytica trophozoites and accelerated amebic lysis
189 thogenicity factors by which trophozoites of E. histolytica exert their remarkable cytolytic and tiss
191 roteases degrade the key adherence lectin on E. histolytica trophozoites and decrease their adherence
193 preschool age, for infection by the parasite E. histolytica every other day over 9 years and evaluate
194 ose expression was upregulated in phagocytic E. histolytica trophozoites to determine whether these g
200 antigen family that includes the serine-rich E. histolytica protein (SREHP), an amebic vaccine candid
202 of a liver transplant recipient with severe E. histolytica colitis who was successfully treated with
204 s opsonins on apoptotic cells that stimulate E. histolytica phagocytosis, an effect mediated at least
205 at the collectins are ligands that stimulate E. histolytica phagocytosis, we used a flow cytometry-ba
206 histolytica HM-1:IMSS is a virulent strain, E. histolytica Rahman is a nonvirulent strain, and Entam
210 Together, these studies demonstrate that E. histolytica has different vesicles that play a role i
211 nesis of amebic colitis, we demonstrate that E. histolytica trophozoites or their released proteinase
212 /BL6.lpr and C57/BL6.gld mice, we found that E. histolytica-induced apoptosis does not require the Fa
213 s to be at variance with the hypothesis that E. histolytica rather recently lost its mitochondrial or
214 The pattern of polymorphism indicates that E. histolytica reproduces sexually, or has done so in th
216 s factor receptor I gene, we have shown that E. histolytica-induced apoptosis is not mediated by tumo
217 in a mouse model of disease and suggest that E. histolytica induces cell death without using two comm
228 tolytica microplate assay from Remel and the E. histolytica II enzyme-linked immunosorbent assay (ELI
229 dascreen Entamoeba test (R-Biopharm) and the E. histolytica II test (Techlab), and we found that the
230 mpared to the ProSpecT microplate assay, the E. histolytica Quik Chek (Quik Chek) assay exhibited 97.
232 roaches we have (a) cloned and expressed the E. histolytica UDP-galactose transporter in Saccharomyce
234 al gene transfer of bacterial genes into the E. histolytica genome, the effects of which centre on ex
236 alyses inferred a closer relationship of the E. histolytica ADHE to bacterial ADHE than to the G. lam
237 8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lecti
239 e composition bias and repetitiveness of the E. histolytica genome provide a challenge for short-read
240 tage process has been the publication of the E. histolytica genome, from which has come an explosion
242 under a promoter (g34) taken from one of the E. histolytica ribosomal protein (RP-L21) gene copies.
246 assays suggested that auranofin targets the E. histolytica thioredoxin reductase, preventing the red
247 ica II test (Techlab), and we found that the E. histolytica II test detects E. histolytica infections
248 ort in Entamoeba is strong evidence that the E. histolytica organelle housing chaperonin CPN60 repres
249 sensitivity and specificity compared to the E. histolytica II ELISA of 98.0% (95% confidence interva
253 th EhILWEQ and EhBAR appear to contribute to E. histolytica virulence through their function in phago
254 tation enzymes from archaea or eubacteria to E. histolytica probably occurred early, because the sequ
257 discrete mechanisms of innate resistance to E. histolytica depending on the host background and, in
258 he human intestinal IgA antibody response to E. histolytica and E. dispar infection and revealed that
259 ction of IL-1 and IL-8 occurs in response to E. histolytica infection in vivo and as an approach to s
260 rophil-mediated tissue damage in response to E. histolytica infection; this inflammatory cascade can
262 ion of this subunit were more susceptible to E. histolytica infection as measured by culture results,
264 tica, we first defined the phenotypes of two E. histolytica strains, HM-1:IMSS, the prototype virulen
271 tica genome, raising the question of whether E. histolytica MIF (EhMIF) has proinflammatory activity
272 were diagnosed with G. intestinalis, 53 with E. histolytica and/or E. dispar, and 14 with both G. int
276 l xenografts in SCID mice were infected with E. histolytica trophozoites expressing an antisense mess
278 from South Africans naturally infected with E. histolytica were examined by enzyme-linked immunosorb
281 udy, 6.3% of ALA subjects were infected with E. histolytica; the incidence of new E. histolytica infe
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