ライフサイエンスコーパス: E1A protein

1 and tumorigenesis mediated by the adenovirus E1A protein.

2 arkedly diminished by expression of the 12 S E1A protein.

3 nase promoter as efficiently as the wildtype E1A protein.

4 the domain of CBP/p300 that binds adenovirus E1A protein.

5 s examined by in vitro binding to adenovirus E1A protein.

6 een for cellular proteins that interact with E1A protein.

7 activation by cellular factors and the viral E1A protein.

8 terminal binding protein binding site in the E1A protein.

9 E1 and fails to produce the immediate-early E1a protein.

10 he p160 coactivators and with the adenovirus E1A protein.

11 o either E1A-Myc chimeras, or the native 12S E1A protein.

12 not only by Myc, but also by the adenovirus E1A protein.

13 antigen, by oncogenic ras, or by adenovirus E1a protein.

14 qually sensitive to inhibition by adenovirus E1A protein.

15 n of E2F could not be reversed by adenovirus E1A protein.

16 cked in HeLa cells expressing the adenovirus E1A proteins.

17 functionally conserved in E2F and adenovirus E1A proteins.

18 performed with recombinant HPV16 E7 and Ad5 E1A proteins.

19 C terminus of adenovirus early region 1A (Ad E1A) proteins.

20 ssion of CBP/p300 function by the adenoviral E1A protein abolishes c-fos activation by EWS, indicatin

21 Human adenovirus E1A protein abrogated cotransactivation by CBP but an E1

22 The adenovirus large E1A protein activates transcription from early viral pro

23 The adenovirus E1A protein acts within the nucleus to regulate transcri

24 ted i.v. with LPD-E1A, and the expression of E1A protein and effects on tumor growth were assessed.

25 r subunit that interacts with the adenovirus E1A protein and functions in a mitogen-activated protein

26 ated to conserved region 1 of the adenovirus E1A protein and importantly contributes to cellular tran

27 in vivo by overexpression of the adenovirus E1A protein and mutant forms of E1A that lack p300-bindi

28 idenced by inhibition with overexpressed 12S E1A protein and reversal of this inhibition with exogeno

29 because it bound normally to the adenovirus E1A protein and showed nuclear localization by immunosta

30 s (p5, p19, and p40) requires the adenovirus E1a protein and the AAV nonstructural (Rep) proteins.

31 bolished by the NO donors: the appearance of E1A protein and the cleavage of cytokeratin 18 by AVP.

32 Since both the adenovirus E1A protein and transcriptional activation by E2F functi

33 y reduced by cotransfection of the wild-type E1A protein and variably reduced by the cotransfection o

34 his activation is enhanced by the adenovirus E1A protein, and evidence for interaction between E1A an

35 steroid receptor co-activator, as well as by E1A proteins, and attenuated by Smad nuclear interacting

36 ponse as a result of expression of the viral E1A proteins, and this process is suppressed by the vira

37 AP-2 and that the N-terminal amino acids of E1A protein are required for this interaction.

38 The adenoviral E1A proteins are able to promote proliferation and trans

39 The adenovirus E1A proteins are involved in the transcriptional activat

40 Adenovirus E1A proteins are known to induce the expression of p34cd

41 nal machinery is inhibited by the adenovirus E1A protein as a result of E1A's binding to the p300 coa

42 Previously, we reported that adenovirus E1a protein behaves as a tumor suppressor in human cells

43 Distinct E1A protein binding sites mediated repression of muscle-

44 ed with proteasomes and that the larger Ad12 E1A protein binds strongly to at least three components

45 force the general conclusion that adenovirus E1A proteins block IFN signaling pathways by lowering th

46 The adenovirus E1A protein both activates and represses gene expression

47 Additionally, the E1A protein bound to p202 both in vitro and in vivo and

48 repressed by E1A, these studies suggest that E1A protein can repress cellular and viral promoter/enha

49 ll established, however, that the adenovirus E1A protein can sensitize these cells to the induction o

50 Between the two major E1A proteins coded by the 12S and 13S mRNAs, the 13S pro

51 ith the use of purified wild type and mutant E1A proteins confirmed that CBP, p300, and pRB were targ

52 Furthermore, the 289R E1A protein could inhibit the BS69-mediated decrease in

53 We report here that the adenovirus E1A protein creates an S phase environment in quiescent

54 nocytes by treatment with a transducible TAT-E1A protein did not rescue the apoptotic phenotype.

55 The E1A proteins displace repressors of E2F activity (the Rb

56 Adenovirus small early region 1a (e1a) protein drives cells into S phase by binding RB fam

57 sis because it is targeted by the adenovirus E1A protein during oncogenic transformation.

58 Adenovirus type 5 E1A protein (E1A) associates with anti-tumor activities

59 Adenovirus type 12 (Ad12) E1A protein (E1A-12) contains a unique 20-amino-acid spa

60 Human adenovirus type 12 (Ad12) E1A protein (E1A-12) is the key determinant of viral tum

61 This is accomplished by the Ad12 E1A protein (E1A-12), which prevents NF-kappaB from beco

62 ntrast, the nontumorigenic adenovirus type 5 E1A protein (E1A-5) and other E1A-12 mutants lacking the

63 adenovirus-infected cells, the expression of E1A protein enhances the accumulation of p53 during earl

64 ult suggests that an inhibiting effect on wt E1A protein expression and/or E1A function might account

65 e p202 is a target of the adenovirus-encoded E1A protein for functional inactivation.

66 An E1A protein fragment consisting of only the E1A N-termin

67 ort to the idea that the p202 and adenovirus E1A protein functionally counteract each other and E1A p

68 A segment of the adenovirus E1A protein fused to a transformation-defective N-Myc pr

69 MAV-1 E1A mutants expressing no E1A protein grew to titers comparable to wild-type MAV-1

70 The adenovirus type 5 E1A protein has been demonstrated to elicit antitumor ef

71 The adenovirus E1A protein has been implicated in increasing cellular s

72 The adenoviral E1A protein has been implicated in the potentiation of a

73 The adenovirus E1A protein has been shown to bind to p300 and inhibit i

74 The adenoviral type 5 E1A protein has been shown to induce sensitization to di

75 Investigations using E1A proteins have illuminated important paradigms in cel

76 nd DP-1 proteins or microinjected adenovirus E1A protein, however, could induce S phase in cells arre

77 Adenovirus E1A proteins immortalize primary animal cells and cooper

78 t proteins have been shown to bind stably to E1A proteins in adenovirus-infected and -transformed cel

79 that transactivation of the promoter by the E1A proteins in cycling cells is mediated through the tw

80 l gene transfer to express various E2F-1 and E1A proteins in neonatal rat cardiac myocytes that are a

81 adenoviruses to express wild-type and mutant E1A proteins in primary cultures from embryonic day 20 r

82 The targets of E1A protein include the retinoblastoma tumor suppressor

83 Studies with E1A protein indicate that interaction with p300/CBP is r

84 s reduced by competition with the adenovirus E1a protein, indicating that binding occurs through the

85 Here we show that the small E1A protein induces c-Myc by interfering with the protei

86 Adenovirus E1A proteins influence cell growth and phenotype through

87 Furthermore, E1A protein inhibited IRF-3-mediated stimulation of the

88 We show that the adenovirus E1A protein inhibits NO production by decreasing express

89 Here we report that the adenovirus E1A protein inhibits the acetyltransferase activity of C

90 Since adenovirus E1A proteins interact with CBP/p300 proteins, we examine

91 ffinity chromatography studies show that the E1A protein interacts with the DNA binding/dimerization

92 E1a protein interacts with the nuclear acetylases p300,

93 tified by its ability to bind the adenovirus E1A protein, interacts both with gene-specific transcrip

94 The adenovirus E1A protein interferes with regulators of apoptosis and

95 The amino terminus of the adenovirus E1A protein is involved in E1A transforming functions, r

96 property of the adenovirus (Ad) transforming E1A protein is linked to its capacity to induce cellular

97 The adenovirus large E1A (L-E1A) protein is a prototypical transcriptional activator

98 r at 48 degrees C or with the adenovirus 13S E1a protein; (iv) this domain of Tag cannot bind concurr

99 nd p21 through the binding of the adenovirus E1A protein leads to the induction of DNA replication in

100 Immediate early E1A protein levels are unaffected by the drugs, but synt

101 These results support the conclusion that E1A protein mediates optimal transactivation of the huma

102 The adenoviral early region 1A (E1A) protein mediates sensitization to different stimulu

103 Adenovirus E1A protein mimics the effects of Twist by inhibiting th

104 ave previously shown that the adenoviral 12S E1A protein modulates the phosphorylation status of p130

105 engineering the expression of the adenoviral E1A protein, necessary for viral replication, under the

106 Despite data suggesting that the adenovirus E1A protein of 243 amino acids creates an S-phase enviro

107 The adenovirus E1A protein of 243 amino acids has been shown to affect

108 The 243-residue E1A protein of adenovirus induces cellular proliferation

109 We have recently shown that E1A protein of human adenovirus downregulates epidermal

110 amino acid motif, PLDLS, conserved among the E1A proteins of human adenoviruses.

111 The adenoviral E1A protein or a peptide encoding the C/H3 region of p30

112 Here we report that the expression of E1A protein overcame p202-mediated inhibition of cell gr

113 Adenovirus E1A proteins prepare the host cell for viral replication

114 have revealed that human adenovirus-encoded E1A protein promotes cell proliferation through the targ

115 The adenovirus early region 1A (E1A) protein promotes cell immortalization and transform

116 Furthermore, E1A protein relieved p202-mediated inhibition of the spe

117 cted cells expressing equally high levels of E1A protein remained resistant to TRAIL.

118 Adenovirus E1A proteins repress tissue-specific gene expression and

119 ovary and L-cells, HPV E7 and the adenovirus E1A protein repressed the transduced hPCNA promoter, but

120 , we demonstrated that the adenovirus type 5 E1A proteins repressed stimulation by the E-box activato

121 found that overexpression of the adenovirus E1A protein represses expression of a reporter gene dire

122 nds to Stat1 and CBP/p300, and by the mutant E1A protein RG2, which binds to Stat1 but not to CBP/p30

123 18 and the oncogenic adenovirus (Ad) type 12 E1A protein share the ability to down-regulate the expre

124 ino acids in the conserved region 2 (CR2) of E1A protein significantly reduced the binding of E1A to

125 d an active canonical NES element within the E1A protein spanning amino acids 70-80.

126 R3 activation domain of the human adenovirus E1A protein stimulates transcription by forming protein-

127 egion 1 (CR1) of the adenovirus type 5 (Ad5) E1A protein synergizes with CR3 in the transactivation o

128 otein functionally counteract each other and E1A protein targets p202 to promote cell proliferation.

129 teins, we analyzed the functional regions of E1A proteins that are required for muscle gene repressio

130 xpression of wild-type and mutant adenovirus E1a proteins that sequester p300/CBP eliminated IFN-gamm

131 /p300-binding is required for the adenovirus E1A protein to regulate transcription of many genes duri

132 protein also cooperates with the adenovirus E1A protein to transform primary baby rat kidney cells,

133 These E1A mutations preclude binding of E1A proteins to p300 and pRB.

134 E1A 243R, as shown by the ability of mutant E1A proteins to repress the promoter, and correlated wit

135 n the MDA-MB-361 breast model, expression of E1A protein was detected in the tumors after LPD-E1A tre

136 Western blot analysis showed that E1A protein was expressed in tumor tissue and immunohist

137 ted through direct binding by the adenovirus E1A protein, we overexpressed E1A in cardiac myocytes us

138 While E1A proteins were not detected in this complex, to our s

139 The adenovirus E1A protein, which also binds to both Rb and TAFII250, e

140 A interference, and expression of adenovirus E1A protein, which inactivates TBP.