ライフサイエンスコーパス: E2 protein

1 ks this ubiquitylation, thus stabilizing the E2 protein.

2 tethered to cellular chromatin by the viral E2 protein.

3 omplexes of HPV-11 DNA ori bound by purified E2 protein.

4 of the hypervariable region I within the HCV E2 protein.

5 repressed by the bovine papillomavirus (BPV) E2 protein.

6 g of Brd4 was detected in the absence of the E2 protein.

7 ain, but not the DNA binding function of the E2 protein.

8 he C-terminal domain that interacts with the E2 protein.

9 hment to mitotic chromosomes by means of the E2 protein.

10 tion, leading to targeted degradation of the E2 protein.

11 onal change that leads to degradation of the E2 protein.

12 tegy using transcriptional regulation by the E2 protein.

13 ng them to mitotic chromosomes via the viral E2 protein.

14 surrounding the phosphorylation sites of the E2 protein.

15 contacts between residues across the entire E2 protein.

16 inding site derived from studies of the BPV1 E2 protein.

17 (BPV1) E2 proteins than it is to the HPV 16 E2 protein.

18 imal DNA-binding domain (DBD) from the HPV 6 E2 protein.

19 to interferon could be mediated through the E2 protein.

20 pulsing DCs with full-length recombinant PDC-E2 protein.

21 rus that expresses the bovine papillomavirus E2 protein.

22 ression is controlled by the papilloma virus E2 protein.

23 ulates gene expression by the papillomavirus E2 protein.

24 ssette in subclones expressing the wild-type E2 protein.

25 V E2 function through phosphorylation of the E2 protein.

26 ication is TopBP1, a known interactor of the E2 protein.

27 an be boosted with a single dose of purified E2 protein.

28 , and F(442), within the same epitope of the E2 protein.

29 ed epitopes for the neutralizing MAbs on the E2 protein.

30 teracting partner of the papillomavirus (PV) E2 protein.

31 nsrepression is common to different types of E2 proteins.

32 ates with pE2, a precursor containing E3 and E2 proteins.

33 onstrating that HCV virions contain apoE and E2 proteins.

34 y be a shared property of all papillomavirus E2 proteins.

35 g affinity than those displayed by the other E2 proteins.

36 together the more divergent BPV-1 and HPV-18 E2 proteins.

37 generated with some of the same recombinant E2 proteins.

38 ift from N417 to N415 in the N417S and N417T E2 proteins.

39 bovine (BPV) and human (HPV) papillomavirus E2 proteins.

40 es consists of three steps: 1) binding of an E2 protein, 2) transfer of ubiquitin from E2 to the HECT

41 individual alanine substitutions across the E2 protein (355 positions) on antibody recognition.

42 cleocapsid and the cytoplasmic domain of the E2 protein, a component of the viral E1/E2 glycoprotein

43 studies confirm that both high- and low-risk E2 proteins adapt their structures on binding to DNA, al

44 We found that although different types of E2 proteins all exhibited transactivation and repression

45 ein is 10,000-fold more immunogenic than the E2s protein alone.

46 Expression of the E2 protein also led to posttranscriptional increase in t

47 ctive program through phosphorylation of the E2 protein although this is unlikely to occur through th

48 GBV-C E2 protein and a synthetic peptide representing the inhi

49 leomorphic and that some of them contain HCV E2 protein and apolipoprotein E on their surfaces.

50 tential binding interface formed between the E2 protein and CD81.

51 Interaction between the E2 protein and E1 helicase of human papillomaviruses (HP

52 es from its heterodimer interaction with the E2 protein and forms a target membrane-inserted E1 homot

53 sibility of structural mimicry between GBV-C E2 protein and HIV-1 particles.

54 from its heterodimeric interaction with the E2 protein and induces the formation of a stable E1 homo

55 les from GBV-C E2-expressing cells contained E2 protein and inhibited TCR signaling in bystander T ce

56 lls that were initially activated by the HCV-E2 protein and might explain the association between HCV

57 length human papillomavirus type 11 (HPV-11) E2 protein and showed that the resultant fusion, called

58 ase enzyme activity, E1 binding to the viral E2 protein and to cellular DNA polymerase alpha-primase

59 ly active subclones expressing the wild-type E2 protein and transcriptionally silent subclones expres

60 eplication requires activities of the E1 and E2 proteins and a DNA segment containing their binding s

61 s in S phase, increases the half-life of the E2 protein, and promotes chromatin binding from S phase

62 d for GBV-C RNA and antibodies against GBV-C E2 protein, and responses to HAART were evaluated.

63 on of the human papillomavirus type 8 (HPV8) E2 protein are required for this binding.

64 The E1 and E2 proteins are both required for papillomavirus DNA rep

65 The papillomavirus E1 and E2 proteins are essential for viral genome replication.

66 The HPV8 E2 proteins are highly phosphorylated, and serine 253 is

67 The papillomavirus E2 proteins are indispensable for the viral life cycle,

68 Previous studies have shown that PV E2 proteins are short lived; however, the mechanisms reg

69 The E2 proteins are transcription/replication factors from p

70 racterized human papillomavirus (HPV) type 6 E2 protein as a model system.

71 the cottontail rabbit papillomavirus (CRPV) E2 protein as a transcriptional regulator because of the

72 2 open reading frame encodes the full-length E2 protein as well as an alternatively spliced product c

73 (FGFR3) coimmunoprecipitated with the BPV-1 E2 protein, as did human papillomavirus 31 (HPV-31) E2,

74 nalysis indicated that the HCV Core, E1, and E2 proteins assembled to form HCV-like particles (HCV-LP

75 lomaviruses since a number of papillomavirus E2 proteins associate with mitotic chromosomes independe

76 e (Y) in the bovine papillomavirus 1 (BPV-1) E2 protein at amino acid 102.

77 ntly identified two antibody epitopes in the E2 protein at residues 412-426 (epitope I) and 434-446 (

78 expression of CHIKV nsP1, nsP3, capsid, and E2 proteins at a concentration as low as 2.5 muM.

79 The betapapillomavirus E2 proteins bind to pericentromeric regions of host mito

80 Thus, the papillomavirus E2 proteins bind to transcriptionally active cellular ge

81 The papillomavirus E2 proteins bind with high affinity to palindromic DNA s

82 th solutions lacking Mg(2+) is observed upon E2 protein binding to sites containing the AATT, TTAA or

83 totic cells before fixation results in alpha-E2 proteins binding to the pericentromeric region of met

84 BZLF1 protein-transactivating domain and the E2 protein-binding domain was able to reactivate lytic r

85 the mutation in CBL-b was located in the Ub-E2 protein-binding RING finger.

86 ast, the human papillomavirus type 8 (HPV-8) E2 protein binds as large speckles at the pericentromeri

87 We have reported that the papillomavirus E2 protein binds the nuclear factor AMF1 (also called G-

88 k and low-risk HPV types, the papillomavirus E2 protein binds to four sites within the viral long con

89 ecific stages of mitosis, the papillomavirus E2 protein binds to MKlp2, and infer that association wi

90 lomavirus human papillomavirus type 8 (HPV8) E2 protein binds to the repeated ribosomal DNA genes tha

91 parate the closely related HPV-16 and HPV-18 E2 proteins but classify together the more divergent BPV

92 e that this is the authentic target of these E2 proteins but that additional factors or a specialized

93 cassette in subclones expressing the mutant E2 protein, but only into the protein binding region.

94 ubiquitination and rapid degradation of the E2 protein by the proteasome pathway.

95 tivities of the bovine papillomavirus E1 and E2 proteins by modifying their DNA binding activity.

96 The papillomavirus E2 protein can silence the long control region (LCR) pro

97 We propose that sequential E2 proteins catalyze K48-linked polyubiquitination and t

98 be a general and important characteristic of E2 protein chemistry.

99 The E2 protein consists of an amino-terminal (N) trans-actin

100 In the absence of other viral proteins, an E2 protein containing alanine substitutions for phosphor

101 lt, ATP, and DTT using full-length E2 and an E2 protein containing only the carboxyl-terminal DNA bin

102 terminal residues, suggesting that the GBV-C E2 protein contains a single immunodominant antigenic si

103 The papillomavirus E2 protein controls primary transcription and replicatio

104 The HPV E2 protein controls replication, transcription, and vira

105 aromyces cerevisiae to determine whether the E2 protein could maintain plasmids containing the yeast

106 mutagenesis, to identify residues in the HCV E2 protein critical for MAb AP33 binding.

107 E2 proteins defective in the transactivation and replica

108 ey are tolerant at the CTL level against the E2 protein despite DNA immunization.

109 However, the HPV-11 E2 protein did not associate with Brd4 during mitosis.

110 and activation of the Rb family, and the BPV E2 protein did not directly affect the expression of cel

111 Using a solid-phase immunoassay, recombinant E2 protein did not interact with LDL.

112 s forming the structural basis of the E1 and E2 protein dimers.

113 , the cancer-associated human papillomavirus E2 proteins display a unique ability to detect DNA flexi

114 ing has been observed in X-ray structures of E2 protein-DNA complexes.

115 Only the alpha-papillomavirus E2 proteins do not stably associate with mitotic chromat

116 domain, that the identity of the cooperating E2 protein does not influence the chain type specificity

117 that MKlp2 specifically associates with the E2 protein during mitosis.

118 e diverse regulatory functions of the HPV-11 E2 protein during mRNA transcription and viral DNA repli

119 However, in the presence of the E2 protein, E2 and Brd4 colocalized in punctate dots tha

120 , mapped between residues 427-446 within the E2 protein, elicits antibodies that are either neutraliz

121 The E2 protein encoded by human papillomaviruses (HPVs) inhi

122 d is mediated by its direct association with E2 proteins encoded by cancer-inducing high risk HPV-16

123 To investigate whether E2 proteins encoded by high risk HPVs may function diffe

124 ctors, cofactor, RNA polymerase II, and with E2 proteins encoded by HPV-16, HPV-18, HPV-11, and bovin

125 h risk HPVs may function differentially from E2 proteins encoded by low risk HPVs and animal papillom

126 The full-length E2 protein, encoded by human papillomaviruses (HPVs), is

127 A boost with soluble E2 protein enhanced titers of neutralizing antibody agai

128 The E2 protein exhibits approximately 30% amino acid identit

129 could provide a mechanism to regulate E1 and E2 protein expression and DNA replication during differe

130 or the viral early protein E2, we found that E2 protein expression did not enhance the intracellular

131 iated by Brd4 interaction with virus-encoded E2 protein, facilitates viral genome segregation during

132 ing affinities of human papillomavirus (HPV) E2 proteins for different E2 binding sites have been pro

133 esents the first expression of a type 2 BVDV E2 protein from a mammalian virus vector and raises the

134 However, when we attempted to express the E2 protein from type 2 (890 strain) BVDV in a bovine her

135 c particles presented the full-length E1 and E2 proteins from a genotype 1a virus in an appropriate c

136 In this study, we show that E2 proteins from a wide range of papillomaviruses intera

137 Although the E2 proteins from all characterized papillomaviruses bind

138 Therefore, E2 proteins from both HPV and animal papillomavirus bind

139 The E1 and E2 proteins from bovine papillomavirus bind cooperativel

140 The E1 and E2 proteins from bovine papillomavirus bind cooperativel

141 Our previous studies of E2 proteins from different genera of papillomaviruses ha

142 Preferences of E2 proteins from different papillomavirus strains for fl

143 However, E2 proteins from different papillomaviruses interact wit

144 In an attempt to identify antibodies to E2 proteins from divergent HCV isolates, we produced HCV

145 l of 78 monoclonal antibodies (MAbs) against E2 proteins from genotype 1a and 2a HCV strains.

146 erty that might distinguish the behaviour of E2 proteins from high- and low-risk HPV subtypes.

147 During persistent infection, the E2 proteins from many papillomaviruses act as molecular

148 In summary, the E2 proteins from many papillomaviruses, including the cl

149 whereas the 3B4C-4 Fab fragment cross-links E2 proteins from neighboring spikes.

150 ocalization of 13 different animal and human E2 proteins from seven papillomavirus genera, and we sho

151 We find that when coexpressed, the E1 and E2 proteins from several papillomavirus types localize t

152 Two of these bound E2 proteins from strains representative of HCV genotypes

153 dy we analyzed how phosphorylation regulates E2 protein function.

154 The papillomavirus E2 protein functions in viral transcriptional regulation

155 function by UVB is due to a reduction of the E2 protein half-life.

156 together, these data indicate that the GBV-C E2 protein has a structural motif that elicits Abs that

157 To date, only the BPV1 E2 protein has been shown to bind to mitotic chromosomes

158 lymphoma immunoglobulin test cases bound the E2 protein in a manner identical to a bona fide human an

159 Expression of the bovine papillomavirus E2 protein in cervical carcinoma cells represses express

160 squitoes, suggesting differing roles for the E2 protein in different hosts.

161 S dipeptides in the hinge region of the HPV1 E2 protein in in vitro kinase assays and that HPV1 E1^E4

162 e data suggest a significant role of the HPV E2 protein in regulating late events in the HPV life cyc

163 creased acetylation and stabilization of the E2 protein in the absence of SIRT1.

164 played by the W(437)LAGLF(442) helix of the E2 protein in the hydrophobic interaction with the D-hel

165 biological activities of the papillomavirus E2 protein in transcription, replication, and maintenanc

166 Furthermore, E2 proteins in which the C-terminal domains were replace

167 We have discovered that E2 proteins, including Ubc1, Ubc2, Ubc4, and Ubc5, can i

168 eated immunization, the response to the rPDC-E2 protein increased with a gradual reduction in autoant

169 Therefore, HCV E2 protein indeed involved in the pathogenesis of type 2

170 with mapping data from other UEV and related E2 proteins indicates that although the different E2/UEV

171 We show that HCV E2 protein induces rapid ezrin phosphorylation and its c

172 The wild-type E2 protein inhibited expression of a luciferase gene lin

173 GBV-C E2 protein inhibits HIV-1 entry, and an antigenic peptid

174 ious reports that recombinant, truncated HCV E2 protein inhibits NK cell functions via crosslinking o

175 The E2 protein interacts with several proteins involved in t

176 Furthermore, the E2 protein interacts with the repeated ribosomal DNA gen

177 -minus E1 protein was cotransported with the E2 protein into the nucleus and supported transient vira

178 The papillomavirus E2 protein is a critical viral regulatory protein with t

179 The E2 protein is a dimeric beta-barrel and the E2-DNA inter

180 The papillomavirus (PV) E2 protein is a DNA binding, protein interaction platfor

181 HPV E2 protein is an attractive candidate for vaccine develo

182 The papillomavirus (PV) E2 protein is an important regulator of the viral life c

183 The human papillomavirus (HPV) E2 protein is an important regulator of viral E6 and E7

184 utant genomes, neither the viral DNA nor the E2 protein is detected on mitotic chromosomes, while oth

185 The transactivation domain of the E2 protein is necessary and sufficient for association o

186 The papillomavirus E2 protein is required for viral transcriptional regulat

187 The E2 protein is responsible for receptor binding and prote

188 The E2 protein is synthesized as a precursor p62, whose proc

189 We show here that the E2 protein is ubiquitinated and degraded by the proteaso

190 with exons 3 and 4 encodes MeCP2-e1 or MeCP2-e2 protein isoforms with unique amino termini.

191 criptional control of papillomavirus-encoded E2 protein, it is unclear how Brd4 regulates E2 function

192 These results suggest that the E2 protein level is regulated by phosphorylation, which

193 on of miR_26a markedly down-regulated cyclin E2 protein levels and significantly decreased proliferat

194 The bovine papillomavirus E2 protein maintains and segregates the viral extrachrom

195 ears that the higher selectivity of the HPV6 E2 protein may arise from its limited molecular adaptabi

196 Our study suggests that the function of the E2 protein may be regulated through a direct FGFR3 targe

197 These data provide evidence that the GBV-C E2 protein may contribute to the block in CD4+ T cell ex

198 r anti-E2 antibodies, suggesting that E1 and E2 proteins mediate HCV-LPs binding and, subsequently, t

199 The viral E2 protein mediates viral DNA replication and transactiv

200 Thus, the E2 protein modulates the chromatin association of Brd4 d

201 The phenotypes of E2 proteins mutated in this region indicate that phospho

202 Purified E2 protein obtained by use of PCR and an expression vect

203 The E2 protein of bovine papillomavirus type 1 is tethered t

204 he cellular binding target through which the E2 protein of bovine papillomavirus type 1 links the vir

205 e effect of C3d on the immunogenicity of the E2 protein of bovine viral diarrhea virus (BVDV).

206 binant adenovirus (Ad) vector expressing the E2 protein of cottontail rabbit papillomavirus (CRPV) wa

207 identified as a novel binding partner of the E2 protein of CSFV using yeast two-hybrid screening.

208 e region between residues 427 and 446 of the E2 protein of HCV genotype 1a, and we examined their cap

209 Antibodies to epitopes in the E2 protein of hepatitis C virus (HCV) reduce the viral i

210 X-ray crystal structure of epitope II on the E2 protein of hepatitis C virus, in complex with nonneut

211 The E2 protein of papillomavirus is the key regulator of vir

212 The E2 protein of papillomaviruses is a site-specific DNA bi

213 We previously demonstrated that the E2 proteins of diverse papillomavirus types, including b

214 Here, we show that the E2 proteins of HPV16 and HPV31 control the expression of

215 The E2 proteins of several papillomaviruses link the viral g

216 All five FAbs reacted with soluble E2 protein only in nonreducing gels, indicating that the

217 red for loading of the bovine papillomavirus E2 protein onto chromatin during DNA synthesis.

218 n is required for loading the papillomavirus E2 protein onto mitotic chromosomes and represents a kin

219 ted in reattachment of the viral DNA and the E2 protein onto mitotic chromosomes, suggesting that E1

220 sed in cells expressing either the A4 mutant E2 proteins or wild-type E2, the E2-A4 protein was much

221 articles (1.12 to 1.18 g/cm(3)), recombinant E2 protein, or control proteins, we assessed binding to

222 We showed that E2 protein partially inhibited DNA unwinding and that Hs

223 In the presence of both Brd4 and E2 proteins, plasmids with multiple E2-binding sites wer

224 it is well established that the viral E1 and E2 proteins play key roles in controlling viral transcri

225 The HPV E2 protein plays a critical role in this tethering by bi

226 The papillomavirus E2 protein plays an important role in viral transcriptio

227 provide insights on the role the alphavirus E2 protein plays on pathogenesis.

228 us particles with primary patient-derived E1-E2 proteins possessed biophysical properties comparable

229 mune responses with recombinant HCV envelope E2 protein produced in mammalian cells.

230 ate that human but not bovine papillomavirus E2 proteins recognize this sequence.

231 The papillomavirus E2 proteins regulate the transcription of all papillomav

232 The papillomavirus E2 proteins regulate viral replication, gene transcripti

233 The bovine papillomavirus E2 proteins regulate viral transcription, replication, a

234 The viral E2 protein regulates transcription from the viral genome

235 Therefore, the viral E2 protein relocalizes and/or stabilizes the association

236 MAb AP33 bound to a panel of functional E2 proteins representative of genotypes 1-6 with higher

237 Expression of the E2 protein resulted in rapid repression of HPV E6 and E7

238 host-cell genome disrupts the HPV regulatory E2 protein, resulting in a loss of negative feedback con

239 nce of the papillomavirus genome rely on the E2 protein's ability to bind that genome specifically.

240 The E2 protein's capacity to bind E2BS in vitro is inhibited

241 llowed by catalyzing Ubl transfer to cognate E2 protein(s).

242 We found that soluble HCV E2 protein (sE2) produced in insect cells is distinctly

243 The E2 protein segregates episomal bovine papillomavirus (BP

244 The E2 protein serves these functions by tethering papilloma

245 Furthermore, the A301 E2 protein shows greatly reduced ubiquitination and degr

246 substrate without the addition of exogenous E2 protein, suggesting that the ubiquitylation of some p

247 n-induced bending were bound more tightly by E2 proteins, supporting the indirect readout model.

248 We show that the HPV E2 protein targets Rad50-interacting protein 1 (Rint1) t

249 In contrast to BPV-1 E2, the HPV-8 E2 protein targets the short arms of acrocentric mitotic

250 vine papillomavirus type 1 (BPV1), where the E2 protein tethers the viral genome to mitotic chromosom

251 rsistent papillomavirus infection, the viral E2 protein tethers the viral genome to the host cell chr

252 The bovine papillomavirus E2 protein tethers the viral genomes to mitotic chromoso

253 The papillomavirus E2 protein tethers viral genomes to host mitotic chromos

254 V 18 and bovine papillomavirus type 1 (BPV1) E2 proteins than it is to the HPV 16 E2 protein.

255 ionally silent subclones expressing a mutant E2 protein that binds its target DNA but is unable to ac

256 of infectious HCV, we mapped regions of the E2 protein that influence a key virus-host interaction a

257 Correspondingly, E2 proteins that could dimerize were able to bind to mit

258 These result in truncated NF-E2 proteins that enhance wild-type (WT) NF-E2 function a

259 In the presence of the E2 protein, these activities became localized to nuclear

260 tudy, we show the localization of the HPV-11 E2 protein to be dynamic.

261 ted that H77.39 inhibited binding of soluble E2 protein to both CD81 and SR-B1, J6.36 blocked attachm

262 aining protein 4 (Brd4) interacts with viral E2 protein to mediate papillomavirus (PV) genome mainten

263 Addition of purified recombinant GBV-C E2 protein to primary human CD4+ and CD8+ T cells inhibi

264 questions, we used the bovine papillomavirus E2 protein to repress the expression of either the E6 pr

265 n of the bovine papillomavirus type 1 (BPV1) E2 protein to the catalytic domain of the FokI restricti

266 apped the major phosphorylation sites of the E2 proteins to serine residues 298 and 301 and shown tha

267 iral genome levels and, like the full-length E2 protein, to repress transcription from the viral prom

268 the correct ubiquitin-conjugating enzyme, or E2 protein, to the target substrate.

269 inal signal sequence region of cdE2 affected E2 protein transport to the plasma membrane, while nonbu

270 The E2 proteins undergo posttranslational modifications that

271 x and subsequent release of the unconjugated E2 protein upon ubiquitin transfer to a substrate or ubi

272 This study reports vaccination against E2 protein using a rabbit model of papillomavirus infect

273 ch an initial step is the association of the E2 protein via a cytoplasmic endodomain with the preasse

274 tor serine residues, the localization of the E2 protein was altered in primary human keratinocytes; w

275 The purified E2 protein was capable of inhibiting membrane-associated

276 The region on the E2 protein was characterized and revealed a highly conse

277 ding site for the human papillomavirus (HPV) E2 protein was determined from an unbiased set of degene

278 ion between the nucleocapsid protein and the E2 protein was explored in solution using NMR spectrosco

279 olocalization of HSC70 with the HCV core and E2 proteins was observed around lipid droplets.

280 imental values obtained with three different E2 proteins, we believe this to be a general and importa

281 Using a panel of mutated E2 proteins, we determined that plasmid stability requir

282 f E7 or E6/E7, p21cip1, cyclin E, and cyclin E2 proteins were all up-regulated.

283 The mutated E2 proteins were assessed for mitotic chromosome binding

284 The bovine papillomavirus (BPV) E2 protein, when phosphorylated by CK2 on two specific s

285 ssion involves the reexpression of the viral E2 protein which is usually deleted in HPV-positive canc

286 double-stranded DNA viruses that encode the E2 protein, which controls transcription, replication, a

287 motile internal puncta that colocalized with E2 protein, which may represent the transport machinery

288 omosome anchor for the bovine papillomavirus E2 protein, which tethers the viral episomes to host mit

289 1bs was flexible relative to the rest of the E2 protein, which was further confirmed by MD simulation

290 ability of E2 to interact with Brd4, but an E2 protein with a mutation that disrupted C-terminal dim

291 A, and this modulates the interaction of the E2 protein with cellular chromatin.

292 of fusion proteins of the hepatitis C virus E2 protein with glutathione S-transferase (GST-E2) or FL

293 Incubation of HCV E2 protein with human and bovine LPs (very low density,

294 essary and sufficient for association of the E2 protein with mitotic chromosomes.

295 ctional complexity of antibodies against HCV E2 protein with neutralizing potential.

296 Exclusion required the interaction of the E2 protein with the capsid protein, a critical step in v

297 n are important for chromosomal interaction, E2 proteins with amino acid substitutions in each conser

298 The interaction of papillomavirus E2 proteins with cellular Brd4 protein is important for

299 PV16 and bovine papillomavirus type 1 (BPV1) E2 proteins, with the C-terminal region of Tax1BP1 inter

300 The F5 Fab fragment cross-links E2 proteins within one trimeric spike, whereas the 3B4C-