ライフサイエンスコーパス: EF hand

1 t accommodates one calcium ion in its fourth EF-hand.

2 of magnitude tighter than that of the pseudo-EF-hand.

3 lin-like domain (CamLD) with calcium-binding EF hands.

4 th calcium-dependent and calcium-independent EF hands.

5 cs by disrupting beta-sheet coupling between EF hands.

6 tion where the PH domain moves away from the EF hands.

7 are pollen-specific proteins containing two EF-hands.

8 th calcium-dependent and calcium-independent EF-hands.

9 influence the structure of the metal-binding EF-hands.

10 pression irrespective of the presence of the EF-hands.

11 mboid protease that harbours calcium-binding EF-hands.

12 hands 1 and 4 as well as the linkers before EF hand 1 and the linkers between EF hands 4 and 5 and E

13 ation in combination with mass spectrometry, EF hands 1 and 4 as well as the linkers before EF hand 1

14 h formed by the surface of non-metal-binding EF-hand 1, the loop and the exiting helix of EF-hand 2,

15 nity for calcium ions of visinin-like domain EF-hands 1 and 2 (K(d) = 200 +/- 50 nM) was appropriate

16 the carrier and form a static unit, whereas EF-hands 1-3 form a calcium-responsive mobile unit.

17 EF hand 2 is highly accessible; however, EF hand 6 was d

18 Under the experimental conditions employed, EF hands 2 and 6, which are known not to bind calcium, w

19 EF-hand 1, the loop and the exiting helix of EF-hand 2, and the entering helix of EF-hand 3.

20 FA titration experiments revealed that NCS-1 EF-hands 2-4 (EF2-4) contributed to binding with the D2R

21 um oscillations (~125-850 nM), while that of EF-hand 3 (K(d) </= 20 nM) implied occupancy at basal ca

22 elix of EF-hand 2, and the entering helix of EF-hand 3.

23 ha-helix, and the C-terminal lobe containing EF-hands 3 and 4 of VILIP-2 are sufficient to transfer i

24 ers before EF hand 1 and the linkers between EF hands 4 and 5 and EF hands 5 and 6 were identified as

25 ly reduced the affinity of Ca(2+) binding to EF-hand 4 and Ca(2+) sensitivity of the cyclase regulati

26 n the C-terminal portion of GCAP1, including EF-hand 4 and the helix connecting it with the N-termina

27 Metal binding in EF-hand 4 has no role in the primary attachment of GCAP1

28 (or "tug") between the fatty acyl group and EF-hand 4 via the C-terminal helix that attenuates the e

29 y affected the affinity of Ca(2+) binding to EF-hand 4.

30 Unexpectedly, EF-hands 4-8 are involved in dimerization of the carrier

31 and the linkers between EF hands 4 and 5 and EF hands 5 and 6 were identified as regions of conformat

32 EF hand 2 is highly accessible; however, EF hand 6 was determined not to be surface accessible in

33 atory domain containing four calcium-binding EF-hands, a linker loop domain with an amphipathic alpha

34 lcium-regulated N-terminal domain with eight EF-hands, a mitochondrial carrier domain, and a C-termin

35 Interestingly, deleting the amino-terminal EF-hands activates proteolysis prematurely, while residu

36 hemotaxis was unaffected when Ca(2)+-binding EF hands and a conserved phosphorylation site of RGEF-1b

37 Recoverin has two functional EF hands and a myristoylated N terminus.

38 penta EF-hand proteins, FCaBP lacks a fifth EF-hand and is monomeric.

39 The lumenal EF-hand and SAM domains of STIM1 are believed to initiat

40 region responsible for the inhibition to the EF-hand and SH2 domains.

41 terminal (PBR-N), located between the fourth EF-hand and the first transmembrane region, and one C-te

42 lasmic Na(V) C-terminal region including two EF-hands and an IQ motif, the Na(V) domain III-IV linker

43 Surprisingly, we find that the C-terminal EF-hands and coiled-coil domains do not contribute to PK

44 t PC2 channels unable to bind Ca(2+) via the EF-hand are inactive in single-channel planar lipid bila

45 to the kinase domain and two calcium-binding EF-hands at its N terminus.

46 al constraints imposed on the ligands by the EF-hand beta-scaffold.

47 but in evolutionarily earlier species, both EF hands bind calcium.

48 Atypically, the C-terminal EF-hand binding loop in Phl p 7 (from timothy grass) har

49 in the radius of gyration (R(g)) of the PC2 EF-hand by small angle x-ray scattering and significant

50 tions, ChREBP interacts with sorcin, a penta EF hand Ca(2+) binding protein, and is sequestered in th

51 model and analogous to crystal structures of EF hand Ca(2+)-binding proteins, two carboxylates (Asp-2

52 S100A6 is a member of the S100 subfamily of EF-hand Ca (2+) binding proteins that has been shown to

53 The BbCal1 sequence contained an EF-hand Ca(2+) binding domain and potential hydrophobic

54 diac gene delivery of parvalbumin (Parv), an EF-hand Ca(2+) buffer, has been studied as a therapeutic

55 We conclude that EF-hand Ca(2+) buffers regulate presynaptic IHC function

56 nses decreased ER luminal Ca(2+) through its EF-hand Ca(2+)-binding motif and aggregates in near-plas

57 re members of the phylogenetically conserved EF-hand Ca(2+)-binding protein superfamily.

58 is a widely expressed, 22-kDa myristoylated EF-hand Ca(2+)-binding protein that shares a high degree

59 EF-hand Ca(2+)-binding proteins are thought to shape the

60 mologous proteins (CHP) are N-myristoylated, EF-hand Ca(2+)-binding proteins that regulate multiple c

61 ber of the neuronal calcium sensor family of EF-hand Ca(2+)-binding proteins.

62 EF-hand Ca(2+)-binding sites were selectively inactivate

63 titution (ParvE101Q) at amino acid 12 of the EF-hand Ca(2+)/Mg(2+) binding loop disrupts bidentate Ca

64 of the parvalbumin family, relative to other EF-hand CaBPs in cochlear and vestibular organs in the m

65 -induced calcium flash activates DUOX via an EF hand calcium-binding motif and thus triggers the prod

66 The S100 family of EF-hand calcium (Ca(2+))-binding proteins is essential f

67 ting of NaV1.5, in part through a C-terminal EF-hand calcium binding domain.

68 Tescalcin, an EF-hand calcium binding protein that regulates the Na(+)

69 The EF-hand calcium binding protein, parvalbumin, is a major

70 Secretagogin (SCGN), a hexa EF-hand calcium binding protein, plays key roles in insu

71 In the inner ear, EF-hand calcium buffers may play a significant role in h

72 nlike other reports of genetic disruption of EF-hand calcium buffers, deletion of oncomodulin (Ocm),

73 S100A6 is a small EF-hand calcium- and zinc-binding protein involved in th

74 ein was found to be nearly identical in both EF-hand calcium-binding domains (RMSD=0.19).

75 Two functional EF-hand calcium-binding motifs mediate a calcium-enhance

76 rvalbumin, calbindin, calretinin, N-terminal EF-hand calcium-binding protein 1, cholecystokinin, reel

77 DREAM (calsenilin/KChIP3) is an EF-hand calcium-binding protein that binds to specific D

78 Here we demonstrate that antibodies for the EF-hand calcium-binding protein, secretagogin, strongly

79 ion when compared with the apo states of the EF-hand calcium-binding proteins calmodulin, S100B, and

80 r to the binding of target peptides to other EF-hand calcium-binding proteins.

81 Mts1 is a member of the S100 family of EF-hand calcium-binding proteins.

82 h about 20 members in humans, is composed of EF-hand calcium-regulated proteins and is linked to a ra

83 and functional studies showed that the first EF hand can only bind calcium and be functionally active

84 We found that the EF-hand changes its conformation upon calcium binding, t

85 Ca(2+) and Mg(2+) binding to the individual EF-hands, characterize metal-induced conformational chan

86 ions of its other three constituent domains: EF-hand, coiled coil, and leucine zipper.

87 motility-signaling connections, since EFHC1 (EF-hand containing 1), a potential PACRG interaction par

88 We characterized an inorganic soluble EF-hand containing pyrophosphatase from T. cruzi (TcVSP)

89 Leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), one

90 oupling proteins 2 and 3, the leucine zipper-EF-hand containing transmembrane protein 1 and the mitoc

91 ne protein 1-independent to a leucine zipper-EF-hand containing transmembrane protein 1- and mitochon

92 cium uniporter-dependent, but leucine zipper-EF-hand containing transmembrane protein 1-independent t

93 Its molecular components include the EF-hand-containing calcium-binding proteins mitochondria

94 LC25A24, and SLC25A25--represent a family of EF-hand-containing mitochondrial proteins that transport

95 The EF-hand-containing protein apoptosis-linked gene 2 (ALG-

96 to identify MICU1 (also known as CBARA1), an EF-hand-containing protein that serves as a putative reg

97 Nox5, an EF-hand-containing reactive oxygen species (ROS)-generat

98 oupling protein 2 (UCP2), and leucine zipper EF-hand-containing transmembrane protein 1 (LETM1).

99 l Na/Ca exchanger) and LETM1 (leucine zipper-EF-hand-containing transmembrane protein 1) were propose

100 mitochondrial protein LETM1 (leucine zipper-EF-hand-containing transmembrane protein).

101 Calmodulin-like (CML) proteins are major EF-hand-containing, calcium (Ca(2+))-binding proteins wi

102 Mutations in the second EF-hand (D61N, D63N, D65N, and E72A) of S100B were used

103 ed Mg(2+) binding in all three metal-binding EF-hands did not affect GCAP1 association with the cycla

104 a missense mutation occurs in the C-terminal EF hand domain (C2384Y).

105 tetrameric oligomeric state in which a fused EF hand domain arranges around the catalytic PPase domai

106 Here we asked which role EF hand domain containing 2 (EFhd2; Swiprosin-1) plays i

107 otion in PLC-beta by cross-linking it to the EF hand domain inhibits stimulation by Gbetagamma withou

108 Here we identified the small EF hand domain-containing protein Ca(2+) and integrin-bi

109 B1-interacting partners identified a related EF hand domain-containing protein, calcineurin B, the re

110 ail consists of three functional regions: an EF-hand domain (PC2-EF, 720-797), a flexible linker (798

111 ns in the cationic residues within the first EF-hand domain and the XY linker region dramatically red

112 the EF-hand motifs or deletion of the entire EF-hand domain did not affect the Ca(2+)-dependent activ

113 racterized the conformational changes in its EF-hand domain due to trimer formation.

114 Although the role of the EF-hand domain in RyR1 function has been studied extensi

115 functional significance of the corresponding EF-hand domain in RyR2.

116 These results indicate that, although the EF-hand domain is not required for RyR2 activation by cy

117 Downstream of the EF-hand domain lies a coiled-coil region, which is invol

118 On the other hand, deletion of the EF-hand domain markedly suppressed the luminal Ca(2+) ac

119 aracterized binding interactions between the EF-hand domain of alpha-actinin (Act-EF34) and peptides

120 Mutation of this EF-hand domain of junctate impaired its Ca(2+) binding a

121 Recombinant EF-hand domain of phospholipase C delta1 has a moderate

122 of the net positive charge within the first EF-hand domain of PLCzeta significantly alters in vivo C

123 r SOICR termination, whereas deletion of the EF-hand domain of RyR2 increased both the activation and

124 were maintained in the absence of the fourth EF-hand domain of the light chain, and were sensitive to

125 h an amino acid substitution in a functional EF-hand domain or a truncation of this motif by aberrant

126 vels, and an immediately adjacent C-terminal EF-hand domain that directly binds Ca(2+).

127 Ca(2+) binding to the cytosolic EF-hand domain triggers conformational changes coupled t

128 ned the solution structure of the C-terminal EF-hand domain using multidimensional heteronuclear NMR.

129 The binding of an IQ motif peptide to the EF-hand domain was characterized by isothermal titration

130 Recently, the PLCdelta1 EF-hand domain was shown to bind to anionic phospholipid

131 a(2+) binding to its canonical binding site (EF-hand domain) of polycystin 2, a Ca(2+)-dependent chan

132 in the PC2 Cterm, there is a calcium-binding EF-hand domain, crucial for the calcium-dependent activi

133 an additional 60 residues C-terminal to the EF-hand domain, including the IQ motif that is central t

134 eptide binds between helices I and IV in the EF-hand domain, similar to the binding of target peptide

135 2 has been shown to contain a Ca(2+)-binding EF-hand domain, the molecular basis of PC2 channel gatin

136 ure has the overall architecture of a paired EF-hand domain, the NaV1.2 C-terminal domain does not bi

137 Lys-53, and Arg-57) within the mouse PLCzeta EF-hand domain.

138 in the SUPC2 Ccore relative to the isolated EF-hand domain.

139 g site in the HPC2 Cterm, located within its EF-hand domain.

140 e intrinsic binding affinity of the isolated EF-hand domain.

141 cooperative Ca(2+)-binding sites within its EF-hand domain.

142 k calcium affinity reported for the isolated EF-hand domain; high affinity binding is observed only i

143 Ectopic expression of SLC25A23 EF-hand-domain mutants exhibits a dominant-negative phen

144 SAXS data suggest structural flexibility in EF hand domains indicative of conformational plasticity

145 With the EF hand domains of Miro1 mutated to prevent Ca2+ binding

146 n-D28k is a calcium binding protein with six EF hand domains.

147 duplication, and possess four Ca(2+)-binding EF hand domains.

148 cTnC contains two EF-hand domains (the N and C domain of cTnC, cNTnC and c

149 Deletions that disrupt core structure of the EF-hand domains abolish LMAN1 binding.

150 The EF-hand domains also mediate the interaction with FV and

151 Here we show that the C-terminal EF-hand domains are both necessary and sufficient for MC

152 fferential calcium binding affinities of the EF-hand domains compared with those of CaM suggest that

153 Calcium binding to the EF-hand domains of CP increases the Mn(II) affinity of t

154 Our results suggest that the EF-hand domains of MCFD2 contain separate binding sites

155 Furthermore, we show that the Ca(2+)-sensing EF-hand domains of Miro1 are important for regulating mi

156 ty to bind free calcium, via calcium binding EF-hand domains on the protein, or to bind calcium compl

157 We find that calcium binding to the EF-hand domains promotes autophosphorylation, which nega

158 ized to different structural elements of the EF-hand domains suggest that Ca(2+)-induced folding is i

159 terminal helix and the linker connecting two EF-hand domains).

160 ium binding and requires functionally intact EF-hand domains.

161 we show that two of the four calcium binding EF-hands dominate the FRET output of TN-XXL and that loc

162 decode Ca(2+) signals using four specialized EF-hands, each with specific, conserved traits.

163 function of the ABD and that the C-terminal EF hands (EF(34)) may bind to the linker that connects t

164 Mg(2+) binds to the first and third EF-hands (EF1 and EF3), and Ca(2+) binds to EF1, EF3, an

165 t structural differences occur in the fourth EF-hand (EF4) and adjacent helical region (residues 164-

166 S100A4 binds two Ca2+ ions with the typical EF-hand exhibiting an affinity that is nearly 1 order of

167 ecretagogin, a recently cloned member of the EF-hand family of calcium binding proteins, was localize

168 H/DX results the deuterium patterns for each EF hand for each state of the protein (apo through fully

169 HLH motif may have evolved from a canonical EF-hand found in invertebrate PC2 homologs.

170 bstitution that had been presumed to destroy EF-hand function, that of glutamine for glutamate at pos

171 minal actin-binding domain and COOH-terminal EF-hand-GAS2 domain.

172 We also found that the four EF-hands have distinguishing biophysical and structural

173 oponin C (TnC) belongs to the superfamily of EF-hand (helix-loop-helix) Ca(2+)-binding proteins and i

174 agnesium cation that binds preferentially to EF-hand I of both isoforms.

175 conserved Phe residues at the -4 position in EF-hand III (F[-4]; Tyr(81)) and at the 13th position in

176 a decrease in the Ca(2+) binding affinity of EF-hands III and IV in OsCaM61.

177 variant, Rasgrp1(Anaef), with an ENU-mutated EF hand in the Rasgrp1 Ras guanine nucleotide exchange f

178 From these results, we suggest that the EF hands in the C-lobe are flexible and can be thought o

179 xt showed that coordination of calcium by an EF-hand in ELC1 and prebinding of MLC1 to the MyoA neck

180 STIM proteins contain an EF-hand in their N-termini that faces the lumen side of

181 The four EF-hands in FCaBP each adopt a "closed conformation" sim

182 different relative binding affinities of the EF-hands in the engineered fragment compared with the in

183 d N-terminal region (residues 1-99) and four EF-hands in two separate lobes.

184 dition, the level of binding of Ca2+ to both EF-hands increases by 1 order of magnitude in the presen

185 ctive if the second (native) calcium-binding EF hand is intact.

186 We propose that the PC2 EF-hand is a Ca(2+) sensor required for channel gating.

187 e propose that after STIM1 loses Ca(2+) from EF hand, its intraluminal SAM domain may change conforma

188 (F[-4]; Tyr(81)) and at the 13th position in EF-hand IV (F[+13]; Phe(129)) of the C-terminal domain.

189 ge that is most prominent around the mutated EF-hand IV, as well as throughout the C-domain.

190 tion destabilises the native conformation of EF-hand IV, leading to a transient unfolding and dissoci

191 The MyoB-specific, single-lobe EF-hand light chain MlcB binds the sole IQ motif of MyoB

192 The structural integrity of the EF-hand like motif is essential for the 14-3-3 protein-m

193 Our data suggest that the EF-hand like motif-containing domain functions as the in

194 CaM)-binding IQ motif and the Ca(2+) sensing EF hand-like (EFL) motif in the carboxyl terminus of the

195 modified beta-propeller fold and a distinct EF-hand-like calcium-binding site conserved in pathogens

196 l growth and has been reported to possess an EF-hand-like domain.

197 cated within the second RCK domain, forms an EF-hand-like motif and is strategically positioned close

198 th1.14-3-3 complex and the importance of the EF-hand-like motif were investigated using site-directed

199 talytic domain and the region containing the EF-hand-like motif, whose role in the activation of Nth1

200 The EF-hand-like motif-containing region forms a separate do

201 This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residu

202 Mutation of predicted EF-hand loop residues in PC2 to alanine abolishes Ca2+ b

203 itions of bound Mg(2+) and Mn(2+) within the EF-hand loops are similar to those of Ca(2+); however, t

204 inase-inducible domain peptides are based on EF-hand loops in which a structurally critical Glu12 res

205 ain does not bind Ca2+ through the canonical EF-hand loops, as evidenced by monitoring 1H,15N chemica

206 cture and the absence of Ca2+ binding by the EF-hand loops.

207 among its four Ca(2+)-binding sites, called EF-hands, may contribute to CaM's functional versatility

208 exhibits low homology (30-40%) to the pseudo-EF-hand mitochondrial SCaMCs buffering/solute transport

209 It lacks the EF hand motif characteristic of classical calpains but r

210 To test whether the EF hand motif is truly a calcium sensor controlling PC2

211 A server predicts two domains as follows: an EF-hand motif (PC2-EF) connected by a linker to a previo

212 )/Mg(2+) binding affinities of parvalbumin's EF-hand motif alter function of cardiac myocytes.

213 Strategic design of new EF-hand motif domains to modulate intracellular Ca(2+) s

214 The Ca(2+)-binding EF-hand motif in Cdc24, the Cdc42 activator, was essenti

215 Furthermore, we identified a Ca(2+)-binding EF-hand motif in the ER-luminal region of junctate.

216 The Cdc24 EF-hand motif is therefore essential for polarity establ

217 ently accepted model of PC2-C consists of an EF-hand motif overlapping with a short coiled coil; howe

218 on and contains one canonical and one pseudo-EF-hand motif per monomer, each of which consists of two

219 Here, an EF-hand motif substitution that had been presumed to des

220 ation sequences (NLS) as well as a conserved EF-hand motif that binds the Wnt receptor-associated sca

221 alpha-helix (H6) and the proximal structured EF-hand motif using transition-metal ion fluorescence re

222 if packed against a canonical Ca(2+)-binding EF-hand motif.

223 n of a putative intracellular Ca(2+)-binding EF-hand motif.

224 ity, we focused here on amino acid 12 of the EF-hand motif.

225 Here, we investigate whether these EF hand motifs serve as a calcium sensor responsible for

226 The C-terminal tail of hPC2 contains 2 EF hand motifs, but only the second binds calcium.

227 Ca(2+) binding domain encompassing a pair of EF-hand motifs (EF1 and EF2) in the skeletal muscle ryan

228 ) superfamily that harbors two high affinity EF-hand motifs and a C-terminal transmembrane domain.

229 AM structure (residues 78-256) contains four EF-hand motifs arranged in a tandem linear array, simila

230 y of Ca(2+) signaling in health and disease, EF-hand motifs designed to have new biological activitie

231 We also show that the EF-hand motifs of Miro mediate Ca(2+)-dependent arrest o

232 The two non-functional EF-hand motifs of MlcC pack together to form a globular

233 e investigate the effect of mutations in the EF-hand motifs on the Ca(2+) activation of RyR2.

234 We found that mutations in the EF-hand motifs or deletion of the entire EF-hand domain

235 of 10(8) and 10(4) M(-1), respectively, both EF-hand motifs qualify as Ca(2+)/Mg(2+) sites.

236 typical structure of CaMs, with two pairs of EF-hand motifs separated by a short linker domain.

237 of Medicago truncatula CCaMK, which contains EF-hand motifs, or this domain together with the autoinh

238 toyl group on a long N-terminal arm and four EF-hand motifs, three of which bind Ca2+, assembled into

239 tionary time, focusing primarily on the four EF-hand motifs.

240 293 cell line expressing the STIM1-D76A/E87A EF-hand mutant (STIM1(EF)) deficient in Ca(2+) binding.

241 Microinjection of the PLCzeta EF-hand mutants into mouse eggs enabled their Ca(2+) osc

242 ent compared with the intact odd-even paired EF-hands (nCaM and cCaM) in terms of changes in flexibil

243 Ca(2+) and Mg(2+) binding to the individual EF-hands, observed metal-induced conformational changes,

244 mine the order of Ca(2+) binding of the four EF hands of the protein.

245 Specifically, loss of calcium from the EF-hand of STIM1 that forms the key initiation point for

246 studies revealed that Ca(2+) binding to the EF-hands of hSCGN induces significant structural changes

247 we found that in the presence of Ca(2+) the EF-hands of human plastins bound to an immediately adjac

248 tial pathway that favors the calcium binding EF-hands of the C-terminal lobe over those of the N-term

249 g in the high-affinity Ca(2+)-binding sites (EF-hands) of the GUCA1A gene encoding guanylate cyclase-

250 ions suggests binding sites that are not an EF-hand or calmodulin model.

251 ane helices interacts intimately with paired EF-hands originating from the alpha-solenoid scaffold, s

252 elate with increased molecular motion in the EF-hand, possibly due to exchange between apo and Ca(2+)

253 as a point mutation in the calcineurin-like EF hand protein 1 (Chp1) gene that resulted in the produ

254 We found that the penta-EF-hand protein ALG-2 binds to the NH-terminal cytosolic

255 n neuronal calcium sensor-1, a multispecific EF-hand protein involved in neurotransmitter release and

256 Here, we studied the function of At-MICU, an EF-hand protein of Arabidopsis thaliana with homology to

257 that F1 TnC is a typical collapsed dumbbell EF-hand protein that accommodates one calcium ion in its

258 1) is an intrinsically disordered "imitator" EF-hand protein that increases the number of calcium car

259 This work focused on S100A11, a dimeric EF-hand protein with two hydrophobic target binding site

260 he absence of Ca(2+), DREAM/KChIP3 binds the EF-hand protein, calcineurin subunit-B.

261 e of Ca(2+), DREAM/KChIP3 interacts with the EF-hand protein, calmodulin (CaM).

262 teraction of DREAM/KChIP3 with the important EF-hand protein, CaM, and show that the interaction alte

263 t conformational change into a high-affinity EF-hand protein, furnishing a mechanism by which the pro

264 Parvalbumin (Parv), the archetypal EF-hand protein, is a high-affinity Ca(2+) buffer in man

265 onistic modulator (DREAM/KChIP3), a neuronal EF-hand protein, modulates pain, potassium channel activ

266 from Entamoeba histolytica, is a two-domain EF-hand protein.

267 nctions of the members of the S100 family of EF-hand proteins are modulated by calcium and, in a numb

268 EF-hand proteins are ubiquitous in cell signaling.

269 ff of calmodulin activity and possibly other EF-hand proteins at the resting levels of Ca(2+).

270 S100B is a member of the S100 subfamily of EF-hand proteins that has been implicated in malignant m

271 Unlike the dimeric penta EF-hand proteins, FCaBP lacks a fifth EF-hand and is mon

272 important of these "calcium sensors" are the EF-hand proteins.

273 nding the conformational dynamics of similar EF-hand proteins.

274 at native sm-titin interacts with C-terminal EF hand region and central rod R2-R3 spectrin-like repea

275 with a deletion of the entire N-terminal non-EF hand region still retains the LMAN1-binding function.

276 as well as Z-repeat domains that bind to the EF hand region.

277 amino terminus, whereas the C-lobe binds an EF-hand region upstream of the IQ domain.

278 rgets CDPK2 to amylopectin stores, while the EF-hands regulate CDPK2 kinase activity in response to C

279 nal transmission from Ca(2+) to ABD2 through EF-hands remains to be established.

280 Those critical cationic EF-hand residues in PLCdelta1 are notably conserved in P

281 on of the Ca(2+)-binding residues within the EF hands resulted in a complete loss of PLS3 rescue.

282 and location of calcium-binding sites in the EF hand senses the concentration of calcium required for

283 This protein possesses a modified pseudo-EF-hand sequence at the C-terminal end which exhibits lo

284 d sequences within the putative PFMG1 pseudo-EF-hand sequence region.

285 tionic and hydrophobic residues in the first EF-hand sequence.

286 fter substitution of Ca(2+) by Mg(2+) in its EF-hands, stimulates photoreceptor guanylyl cyclase, Ret

287 affect either the binding pocket affinity or EF-hand structure of the binding domain.

288 n protein export is centrin, a member of the EF-hand superfamily of Ca(2+)-binding proteins.

289 calcium binding proteins that belong to the EF-hand superfamily with diverse biological functions.

290 Calmodulin (CaM), a member of the EF-hand superfamily, regulates many aspects of cell func

291 of grancalcin and other members of the penta EF-hand superfamily.

292 ondrial inner membrane and has two canonical EF hands that are essential for its activity, indicating

293 arge reorientation of helix 3 in the typical EF-hand.This reorganization exposes a hydrophobic cleft

294 determined the order of binding to the four EF hands to be III, IV, II, and I by extracting from the

295 abundant allergenic protein domain families (EF-hand, Tropomyosin, CAP, Profilin, Lipocalin, Trypsin-

296 dumbbell-shaped molecule that contains four EF hands (two in the N-lobe and two in the C-lobe) that

297 escence anisotropy (FA) and a panel of NCS-1 EF-hand variants to interrogate the interaction between

298 when either the Miro were depleted or their EF-hand was mutated.

299 The EF hands were essential for PLS3 rescue of smn morphant

300 ding its myristoylation site, and the second EF-hand, which is inactive in Ca(2+) binding, are the ke