ライフサイエンスコーパス: EF-1

1 EF-1 alpha co-migrates with GST-EF-3 on glutathione-Seph

2 EF-1, as in recoverin, does not bind calcium because it

3 When elongation factor 1 (EF-1) from rabbit reticulocytes was examined as substrat

4 eukaryotic elongation factor 1A-1 (eEF1A-1), EF-1-delta, eEF1gamma, 14-3-3epsilon, and 14-3-3zeta/del

5 in as translation elongation factor-1 alpha (EF-1 alpha).

6 dentified active synthetic peptides AG73 and EF-1 from the sequence of laminin alpha1 LG4 for binding

7 coimmunoprecipitation of WNV genomic RNA and EF-1 alpha, using an anti-EF-1 alpha antibody.

8 NV genomic RNA and EF-1 alpha, using an anti-EF-1 alpha antibody.

9 Mg(2+) binds constitutively to CaBP1 at EF-1 with an apparent dissociation constant (K(d)) of 30

10 osomes involves a direct interaction between EF-1 alpha and EF-3.

11 vidence suggest a direct interaction between EF-1 alpha and EF-3.

12 ciation constant for the interaction between EF-1 alpha and WNV 3' SL RNA was calculated to be 1.1 x

13 ys demonstrated that the interaction between EF-1 alpha and WNV 3' SL RNA was specific.

14 However, binding of Ca(2+) to both EF-1 and EF-3 is necessary for ALG-2 interaction with Al

15 ined with partially purified EF-1, with both EF-1 and ribosomes contributing to stimulation of elonga

16 osed patch of hydrophobic residues formed by EF-1 and EF-2 (Leu24, Trp27, Phe31, Phe45, Phe48, Phe49,

17 ndent EF-3-ATPase activity are influenced by EF-1 alpha.

18 nterference of EF-3/anti-EF-3 interaction by EF-1 alpha but not by EF-2.

19 s of the protein synthesis elongation factor EF-1.

20 he binding activity of the WNV 3' SL RNA for EF-1 alpha by approximately 60%.

21 lyclonal antibody to EF-3 immunoprecipitates EF-1 alpha along with EF-3.

22 alanine substitutions within the AG73 (M1), EF-1 (M2, M3), and alpha-dystroglycan binding sites (M4,

23 he48 that includes the EF-hand-related motif EF-1 is essential both for activation of RetGC at low Ca

24 Dephosphorylation of EF-1 alpha by calf intestinal alkaline phosphatase inhib

25 methods, shows that the N-terminal helix of EF-1 is close to the membrane interface.

26 o associates with the bacterial homologue of EF-1 for its replicase function, implicating a possible

27 e interaction indicated that one molecule of EF-1 alpha binds to each molecule of WNV 3' SL RNA.

28 Specific mutations of EF-1 residues in full-length PLC delta1 reduce enzyme ac

29 Phosphorylation of EF-1 resulted in a 2-2.6-fold stimulation of EF-1 activi

30 timulation involves coordinate regulation of EF-1 and ribosomes through phosphorylation by multipoten

31 EF-1 resulted in a 2-2.6-fold stimulation of EF-1 activity, as measured by poly(U)-directed polypheny

32 e also demonstrate that the alpha subunit of EF-1 remains tightly bound to the L protein in the inact

33 s for the beta, gamma, and delta subunits of EF-1 from rabbit were cloned, and proteins of 225, 437,

34 alyze the role of the individual subunits of EF-1 in elongation, the cDNAs for the beta, gamma, and d

35 the expression of the different subunits of EF-1 may contribute to alterations not only in protein s

36 d virions also package all three subunits of EF-1.

37 esults were obtained with partially purified EF-1, with both EF-1 and ribosomes contributing to stimu

38 s and one additional EF-hand-like structure, EF-1, that deviates form the EF-hand consensus sequence.

39 ee regions, encompassing the N-terminus, the EF-1 motif, and the EF-3 motif, were likely involved in

40 chment on LG4 through syndecans and that the EF-1 site is for cell spreading activity through integri

41 GCAP-specific amino acid residues within the EF-1 can create a contact surface for binding GCAP-2 to

42 ound that various point mutations within the EF-1 domain can specifically affect the ability of GCAP-

43 Point mutations within the EF-1 region can interfere with both the activation of th

44 2+) binding to EF-3, while Ca(2+) binding to EF-1 has little effect on surface exposure of hydrophobi

45 Ca(2+) binds to EF-1 with low affinity (K(d) >100 microM), and no Ca(2+)

46 Interaction with EF-1 alpha at the high-activity binding site was sequenc